Eppendorf 5075 User Manual
n
E
Register your instrument!
www.eppendorf.com/myeppendorf
ual075
N)
manual
®
epMotion
5075
Hardware manual
©
Copyright
2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior
permission of the copyright owner.
Trademarks
Eppendorf
®
, the Epppendorf logo, epMotion®, Eppendorf Tubes ®, and epT.I.P.S.® are registered
trademarks of Eppendorf AG.
®
LightCycler
and MagNA Pure® are registered trademarks of Roche Diagnostics.
epMotion VAC sealing foil U.S. Pat. No. 6,666,978
epMotion optical sensor U.S. Pat. No. 6,819,437
epMotion gripping tool U.S. Pat. No. 7,462,327
Registered trademarks are not marked in all cases with
®
or ™ in this manual.
The software of the device (firmware) contains open source software. License information is available on
request from Eppendorf AG.
Only for epMotion M5073, M5073c, 5075m and 5075mc: Limited Use Label License NOTICE TO
PURCHASER; LIMITED LICENSE FOR RESEARCH USE ONLY.
This product and its use may be covered by one or more patents owned by Gen-Probe Incorporated. The
purchase price for this product includes only limited, nontransferable rights under certain claims of certain
patents owned by Gen-Probe Incorporated to use this product for research purposes only. No other rights
are conveyed. Purchaser is not granted any rights under patents of Gen-Probe Incorporated to use this
product for any commercial use. Further information regarding purchasing a license under patents of
Gen-Probe Incorporated to use this product for any other purposes, including, without limitation, for
commercial use, may be obtained by contacting Gen-Probe Incorporated, Attn: Business Development
Department, 10210 Genetic Center Drive, San Diego, California 92121-4362, U.S.A.
5075 900.840-00/062013
Table of contents
epMotion
®
5075
English (EN)
Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
1.5 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
1.6 Revision history. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
2.1.1 epMotion 5075 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
2.1.2 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
2.1.3 MultiCon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
2.2 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
2.2.1 epMotion 5075l and epMotion 5075lg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
2.2.2 epMotion 5075t and epMotion 5075tg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
2.2.3 epMotion 5075v and epMotion 5075vg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
2.2.4 epMotion 5075vt and epMotion 5075vtg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
2.2.5 epMotion 5075m and epMotion 5075mg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
2.2.6 Accessories for all versions of the epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
2.2.7 Accessories for epMotion with epBlue ID. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
2.2.8 Accessories for epMotion with epBlue GxP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
2.3.1 Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
2.3.2 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
2.3.3 Thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
2.3.4 Thermomixer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
2.3.5 Magnetic separation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
2.3.6 Vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
2.3.7 CleanCap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
3
3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
3.1.1 Intended use of the epMotion 5075 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
3.5 Hazard symbols and safety devices on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
4.2 Installing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Table of contents
®
4
epMotion
5075
English (EN)
5 Hardware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.1 Worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.1.1 Layout of the epMotion 5075I worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.1.2 Layout of the epMotion 5075t worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.1.3 Layout of the epMotion 5075v worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
5.1.4 Layout of the epMotion 5075vt worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
5.1.5 Layout of the epMotion 5075m worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.2 Optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.3 Thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
5.3.1 Safety notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
5.3.2 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
5.3.3 Equipping the thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
5.4 Thermomixer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
5.4.1 Safety notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
5.4.2 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
5.4.3 Equipping the thermomixer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
5.5 Magnet finger module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
5.5.1 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
5.5.2 Equipping the magnet finger module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
5.6 Vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
5.6.1 Vac Frame vacuum frame . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
5.6.2 VAC Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
5.6.3 Silicone mat for VAC Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
5.6.4 Vac Thermo Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
5.6.5 Elution plate adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
5.6.6 Loading the vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
5.7 CleanCap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
5.7.1 HEPA filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
5.7.2 UV lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
5.8 Barcode reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
5.9 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
5.9.1 Dispensing Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
5.9.2 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
6 Dispensing liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.1 Basic procedure for dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.1.1 Operating principle of dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.1.2 Liquid aspiration up to the remaining volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
6.1.3 Bottom tolerance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
6.1.4 Transporting and dispensing liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
6.2 Dispensing modes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
6.2.1 Dispensing mode: Pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
6.2.2 Dispensing mode: Multidispense . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
6.2.3 Mixing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
6.3 Special features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
6.3.1 Liquid aspiration from bottom of vessel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
6.3.2 Liquid aspiration from high vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
6.3.3 Liquid dispensing at the height of the vessel edge. . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
6.3.4 Liquid aspiration from a filter plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Table of contents
®
epMotion
5075
English (EN)
7 Labware – vessels, plates and pipette tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
7.1 Tubes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
7.2 Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
7.2.1 Deepwell plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
7.2.2 Microplates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
7.2.3 Filter plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
7.2.4 Channeling plate 96 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
7.2.5 PCR plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
7.2.6 Tube plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
7.3 Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
7.4 epT.I.P.S. Motion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
7.4.1 epT.I.P.S. Motion Racks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
7.4.2 epT.I.P.S. Motion Reloads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
7.4.3 Tip holder for epT.I.P.S. Motion Reloads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
7.4.4 epT.I.P.S. Motion SafeRacks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
8 Labware accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
8.1 Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
8.1.1 Racks for 24 vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
8.1.2 Rack for 96 vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
8.1.3 Rack LC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
8.1.4 Thermorack and Rack 0.5/1.5/2.0 mL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
8.1.5 PrepRack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
8.1.6 ReagentRack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
8.1.7 Reservoir Rack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
8.1.8 ReservoirRack 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
8.1.9 Reservoir Rack modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
8.2 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
8.2.1 Height adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
8.2.2 Vac holder vacuum holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
8.2.3 Thermoadapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
8.2.4 Thermoadapter LC samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
8.2.5 Thermoadapter Frosty. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
8.3 Thermoblocks and Thermoadapters for PCR plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
8.3.1 Equipping the thermoblock with 0.2 mL vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
8.4 Waste container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
8.4.1 Waste container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
8.4.2 Waste funnel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
8.4.3 LiquidWasteTub with lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
5
Table of contents
®
6
epMotion
5075
English (EN)
9 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
9.1 First steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
9.1.1 Switching on the epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
9.1.2 Using the MultiCon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
9.1.3 Using the epMotion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
9.2 Loading the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
9.2.1 Display the loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
9.2.2 Positioning tools on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
9.2.3 Positioning the epT.I.P.S. Motion on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
9.2.4 Positioning the waste container on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
9.2.5 Positioning labware on the worktable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
9.3 Executing an application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
9.3.1 Performing a test run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
9.3.2 Starting the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
9.3.3 Controlling the application run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
9.4 Completing your work. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
9.4.1 Cleaning up the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
9.4.2 Switching off the epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
10 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
10.1 Decontamination and cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
10.1.1 Carrying out wipe decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
10.1.2 Carrying out spray decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
10.1.3 Carrying out UV irradiation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
10.1.4 Autoclaving . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
10.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
10.3 Servicing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
10.4 Servicing the vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
10.4.1 Replacing the seals of the vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
10.4.2 Replacing the silicone mat of the vacuum lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
10.5 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
10.6 Carrying out firmware updates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
10.7 Checking the dispensing volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
10.8 Maintaining the dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
10.8.1 Exchanging the sealing rings on the eight-channel dispensing tool . . . . . . . . . . . . . 103
10.9 Customer service functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
11 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
11.1 Error search . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
11.2 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
11.2.1 Optical sensor errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
11.2.2 Dispensing errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
11.2.3 Software errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
12 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
12.1 Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
12.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
12.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
Table of contents
®
epMotion
5075
English (EN)
13 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
13.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
13.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
13.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
13.4 Noise level. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
13.5 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
13.6 MultiCon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
13.7 Additional specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
13.7.1 Optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
13.7.2 Carrier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
13.7.3 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
13.8 Enhancements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
13.8.1 Thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
13.8.2 Thermomixer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
13.8.3 Magnet finger module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
13.8.4 Vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
13.8.5 Barcode reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
13.8.6 HEPA filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
13.8.7 UV lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
13.9 Dispensing tool errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
13.9.1 Pipetting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
13.9.2 Dispensing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
13.10 Test conditions for dispensing tool errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
7
14 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
14.1 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
14.2 epT.I.P.S. Motion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
14.3 Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
14.4 Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
14.5 Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120
14.6 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
14.7 Thermoblocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
14.8 Accessories for the vacuum chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
14.9 MagSep kits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
14.10 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
14.11 Modification kits and enhancements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
Table of contents
epMotion
8
English (EN)
®
5075
Operating instructions
epMotion
English (EN)
1 Operating instructions
1.1 Using this manual
Your epMotion operating manual consists of hardware instructions and software instructions. Short
instructions are available for optional software enhancements.
The operating manual is part of the product.
®
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9
The current version of the operating manual can be found on our webpage: www.eppendorf.com
.
Read the operating manual in full before using the device.
Store the operating manual at an easily accessible location.
The device may only be transferred with the operating manual.
If the operating manual is lost, replace it immediately. Please contact Eppendorf AG for further details.
1.2 Danger symbols and danger levels
The safety instructions in this manual appear with the following danger symbols and danger levels:
1.2.1 Danger symbols
Biohazard Explosion
Strong magnetic field UV radiation
Electric shock Heavy loads
Cuts Hot surface
Hazard point Material damage
1.2.2 Danger levels
DANGER Will lead to severe injuries or death.
WARN ING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
10
Operating instructions
epMotion
English (EN)
®
5075
1.3 Symbols used
Depiction Meaning
1.
2.
Actions in the specified order
Actions without a specified order
• List:
Text Display or software texts
Additional information
1.4 Abbreviations used
BCR
Barcode reader
DNA
Deoxyribonucleic acid (DNA)
DWP
Deepwell plate
epT.I.P.S.
eppendorf Totally Integrated Pipetting System
HEPA
High-Efficiency Particulate Air (filter)
LH
Liquid handling
MTP
Micro test plate
PCR
Polymerase chain reaction
PDF
Portable Document Format
TMX
Thermomixer
USB
Universal Serial Bus
UV
Ultraviolet radiation
VAC
Vacuum unit
Operating instructions
epMotion
English (EN)
1.5 Glossary
A
Application
Programs for a specific application. An application includes the procedure and the equipping of the
worktable.
B
Barcode reader
Device for scanning a barcode.
C
CleanCap
Optional epMotion equipment. The CleanCap includes a HEPA filter and UV lamp.
®
5075
11
D
Destination labware
Labware that liquid is dispensed into during the application. Destination labware refers to a plate or rack.
Destination position
Position that liquid is dispensed into during the application.
Destination vessel
The vessel into which liquid is dispensed during the application.
Dispensing tool
Tool that aspirates and dispenses liquid. Single-channel dispensing tools and eight-channel dispensing
tools are available for various volume ranges.
E
epBlue
Software for creating and administering applications and labware. The epMotion is controlled using the
software. epBlue is used to manage access to epMotion. epBlue can be expanded. The ID module enables
barcode tracking. The GxP module enables electronic documentation in support of 21 CFR Part 11.
Eppendorf Quality
Eppendorf Quality is an Eppendorf AG purity grade for consumables. Eppendorf Quality meets the
requirements for standard products, e.g., precision, accuracy, wetting behavior and tightness.
epT.I.P.S.® Motion
epMotion pipette tips. Only epT.I.P.S.® Motion can be used on the epMotion for all features. epT.I.P.S.®
Motions are available with or without a filter.
12
Operating instructions
®
epMotion
5075
English (EN)
F
Filling volume
Maximum liquid volume of a labware. The epMotion uses the gripper to transport the labware up to the
filling volume. The epMotion aspirates liquid from the labware up to the filling volume. The filling volume is
higher than the working volume.
G
Gripper
Tool that transports labware.
H
Height adapter
Adapter for low labware. The height differences among the labware will be offset to decrease the tool
holder paths, thereby decreasing the run time of the application as well.
HEPA filter
Air filter. The air filter prevents particles such as dust and germs from the environment entering the
epMotion work room.
I
Intermediate labware
Labware that is used to dispense and aspirate liquids during the application.
Intermediate position
Position that is used to dispense and aspirate liquids during the application.
Intermediate vessel
Vessel that is used to dispense and aspirate liquids during the application.
L
Labware
Racks, plates, tips, etc. which are placed on the worktable.
Location
Area on the worktable where the labware is placed.
M
MagSep
System consisting of the magnetic finger module, thermomixer and PrepRack and reagents for nucleic acid
preparation with the epMotion.
MultiCon
epMotion operator panel. You can control your epMotion using the control unit and the epBlue software.
Operating instructions
epMotion
English (EN)
P
PCR clean
PCR clean is an Eppendorf AG purity grade for consumables. PCR clean meets the requirements for
standard products, e.g., precision, accuracy, wetting behavior, tightness. PCR clean also meets the
requirements with regard to absence of human DNA, DNase, RNase and PCR inhibitors.
Consumables with the PCR clean purity grade are controlled and certified by an external laboratory.
Certificates are available for downloading from our webpage www.eppendorf.com
.
Procedure
Sequence of commands that are executed one after the other. Part of an application.
R
Rack
Mount for tubes or pipette tips.
®
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13
Random error
Precision. Standard deviation of the average value of the dispensed volumes.
Remaining volume
Volume that cannot be aspirated from a vessel. The distance from the pipette tip to the vessel bottom,
defined in the software, and the defined immersion depth of the pipette tip in the liquid, must be observed.
Therefore, the pipette tip cannot aspirate the volume. The remaining volume depends on the vessel
geometry.
Reservoir
Reservoirs are used to hold reagents. Reservoirs are hung in a Reservoir Rack or placed directly on the
worktable.
S
SafeRack
Rack with ep.T.I.P.S. Motion pipette tips. The SafeRack features a partition that prevents the contamination
of adjacent tips. Use the SafeRacks if you would like to use tips several times.
Source labware
Labware that liquid is aspirated from during the application. Source labware refers to a plate or rack.
Source position
Position that liquid is aspirated from during the application.
Source vessel
The vessel from which liquid is aspirated during the application.
Sterile
Sterile is an Eppendorf AG purity grade for consumables. Sterile meets the requirements for standard
products, e.g., precision, accuracy, wetting behavior, tightness. Sterile also meets the requirements with
regard to sterility and freedom from pyrogens.
14
Operating instructions
®
epMotion
5075
English (EN)
Systematic error
Accuracy. Deviation of the average value of the dispensed volumes from the selected volume.
T
Thermal module
Thermal module for labware that is integrated in the worktable.
Thermoadapter
Heat-conductive adapter for holding plates.
Thermoblock
A thermoadapter that is permanently connected to a PCR plate or PCR tube.
Thermorack
Temperable rack for smaller vessels, e.g., Safe-Lock tubes for 0.5 mL, 1.5 mL or 2 mL.
V
Vacuum unit
The vacuum unit uses a vacuum to suck liquid from a filter plate. The vacuum unit consists of a vacuum
manifold, vacuum pump and Vac Frame.
Vessel
Tube or single well of a plate.
W
Work volume
Liquid volume of a labware. The epMotion fills a vessel with low levels of contamination up to the working
volume. The working volume is less than the filling volume.
Worktable
Work surface of the epMotion where labware and tools are placed. In the software, the epMotion worktable
is shown as the epBlue worktable.
Operating instructions
1.6 Revision history
Revision history of the epMotion 5075 hardware operating manual, order no. 5075 900.840.
Version of the operating manual Date Changes
Version 00 June 2013 Created new
epMotion
English (EN)
®
5075
15
16
Operating instructions
®
epMotion
5075
English (EN)
Product description
epMotion
English (EN)
2 Product description
2.1 Main illustration
The epMotion 5075 is available with various features. Details of the worktable for your device (see
Worktable on p. 33).
2.1.1 epMotion 5075
Abb. 2-1: Main illustrati on epMotion 5075I
z
y
x
2
1
3
4
®
5075
17
8
10
9
Fig. 2-1: Main illustration epMotion 5075I
1Carrier
The carrier moves in X-direction, Y-direction and
Z-direction.
2 Tool holder
Holds dispensing tools.
epMotio n 5 07 5
7
6
5
6 Front hood
Safety device for protection from movable, UV
radiation parts and contamination.
7 Thermal module (optional)
Plate for heating and cooling labware.
3Optical sensor
Detects levels, tips and labware.
4CleanCap (optional)
contains HEPA filter and UV lamps
5Worktable
Work surface for tools and labware.
8 Mains power switch
9Mouse
10 MultiCon
Control unit used to control the epMotion.
18
1
2
3
Product description
®
epMotion
5075
English (EN)
2.1.2 Interfaces
Abb. 2-2: Interfaces epMoti on 5075
Fig. 2-2: Interfaces epMotion 5075
1Ethernet
3 Mains power connection
Connection for the cable to the MultiCon
2USB
Connection for a USB storage medium for
firmware updates.
Only devices which meet the requirements of IEC 950/EN 60950-1 (UL 1950) standards may be connected
to the interfaces.
2.1.3 MultiCon
Information can be found in the operating manual of the MultiCon.
Only devices which meet the requirements of IEC 950/EN 60950-1 (UL 1950) standards may be connected
to the interfaces.
Product description
epMotion
English (EN)
2.2 Delivery package
The epMotion is available in various versions. Your device has been equipped according to your
specifications. For some software modules additional accessories are available.
2.2.1 epMotion 5075l and epMotion 5075lg
®
5075
19
Quantity Order no.
(International)
1 5075 000.271 100 V - 240 V, 50 / 60 Hz
or 1 5075 000.275 100 V - 240 V, 50 / 60 Hz
Description
Automated pipetting system epMotion 5075l
main device; with MultiCon, keyboard, mouse,
installed software epBlue
Automated pipetting system epMotion 5075lg
main device; with MultiCon, keyboard, mouse,
installed software epBlue GxP
2.2.2 epMotion 5075t and epMotion 5075tg
Quantity Order no.
(International)
1 5075 000.272 100 V - 240 V, 50 / 60 Hz
or 1 5075 000.276 100 V - 240 V, 50 / 60 Hz
Description
Automated pipetting system epMotion 5075t
main device with thermomixer; with MultiCon, keyboard, mouse,
installed software epBlue
Automated pipetting system epMotion 5075tg
main device with thermomixer; with MultiCon, keyboard, mouse,
installed software epBlue GxP
2.2.3 epMotion 5075v and epMotion 5075vg
Quantity Order no.
(International)
1 5075 000.273 100 V - 240 V, 50 / 60 Hz
or 1 5075 000.277 100 V - 240 V, 50 / 60 Hz
1– Reservoir 400 mL
1 5075 785.005 vacuum frame
1 5075 778.009 adapter for Vac Frame
1 5282 000.018 Gripper
Description
Automated pipetting system epMotion 5075v
main device with vacuum unit; with MultiCon, keyboard, mouse,
installed software epBlue
Automated pipetting system epMotion 5075vg
main device with vacuum unit; with MultiCon, keyboard, mouse,
installed software epBlue GxP
Vac Frame 2
Vac Holder
20
Product description
epMotion
English (EN)
®
5075
2.2.4 epMotion 5075vt and epMotion 5075vtg
Quantity Order no.
(International)
1 5075 000.274 100 V - 240 V, 50 / 60 Hz
or 1 5075 000.278 100 V - 240 V, 50 / 60 Hz
1– Reservoir 400 mL
1 5075 785.005 vacuum frame
1 5075 778.009 adapter for Vac Frame
1 5282 000.018 Gripper
Description
Automated pipetting system epMotion 5075vt
main device with vacuum unit and thermomixer; with MultiCon,
keyboard, mouse, installed software epBlue
Automated pipetting system epMotion 5075vtg
main device with vacuum item and thermomixer; with MultiCon,
keyboard, mouse, installed software epBlue GxP
Vac Frame 2
Vac Holder
2.2.5 epMotion 5075m and epMotion 5075mg
Quantity Order no.
(International)
1 5075 000.270 100 V - 240 V, 50 / 60 Hz
or 1 5075 000.279 100 V - 240 V, 50 / 60 Hz
Description
Automated pipetting system epMotion 5075m
main device with magnet finger module and thermomixer; with
MultiCon, keyboard, mouse, installed software epBlue
Automated pipetting system epMotion 5075mg
main device with magnet finger module and thermomixer; with
MultiCon, keyboard, mouse, installed software epBlue GxP
2.2.6 Accessories for all versions of the epMotion
Product description
epMotion
®
5075
English (EN)
21
Quantity Order no.
(International)
1 - With USB connection cable
1 - With USB connection cable
1
1 - Compatible with the country where the order was placed or defined
1 - For connecting the MultiCon to a network
1- Tool for transport securing device
1 5075 753.006 Waste box
1 5075 900.840 epMotion 5075
1 5073 900.866 software operating manual epBlue with MultiCon
optional 1 5075 751.607 with HEPA air filter and UV-lamp
optional 2-
-
Description
Mouse
Keyboard
Cable
For connecting the MultiCon to the epMotion
Mains power cable
USB-Ethernet adapter
Operating Manual
CleanCap
UV tube
For epMotion with CleanCap
2.2.7 Accessories for epMotion with epBlue ID
Quantity Order no.
(International)
1
1
–
Barcode reader
Description
USB dongle eLicenser
with hardware key code card
With USB connection cable and table stand
2.2.8 Accessories for epMotion with epBlue GxP
Quantity Order no.
(International)
1
1
1 5075 900.874 epBlue GxP
–
–
Description
USB dongle eLicenser
with hardware key code card
Documentation folder
Folder for certificates and documentation
Operating Manual
22
Product description
epMotion
English (EN)
®
5075
2.3 Features
The epMotion 5075 is available with different features. All available features are described in this manual.
2.3.1 Function
The epMotion 5075 is an automatic system for dispensing liquids using dispensing tools and ep.T.I.P.S.
Motion pipette tips.
The epMotion 5075 dispenses liquids in the volume range 1 µL – 1 000 µL.
The dispensing tool aspirates liquid from source vessels and dispenses this liquid in destination vessels.
The dispensing tool works according to the piston-stroke principle. For the epMotion, dispensing tools are
available for 3 different volume ranges.
The epMotion features an optical sensor. The sensor checks:
• Type and location of labware
• Quantity and position of pipette tips in the rack
• Filling level of vessels
You control the epMotion 5075 via the MultiCon control unit with the epBlue software.
You can use the epBlue software to define dispensing processes and compile them into an application. To
do so, you select source vessels and destination vessels, define the procedure and define the transfer
pattern.
2.3.2 Gripper
A gripper is available for the epMotion. The gripper stacks and transports labware.
2.3.3 Thermal module
The epMotion can be equipped with a maximum of 3 thermal modules.
2.3.4 Thermomixer
The epMotion can be equipped with a thermomixer.
Product description
epMotion
®
5075
English (EN)
2.3.5 Magnetic separation
The epMotion can be equipped with a thermomixer and a magnet finger module for separating magnetic
particles.
This epMotion is a system for preparing nucleic acids. MagSep reagent kits are available for this system.
2.3.6 Vacuum unit
The epMotion can be equipped with a vacuum unit.
With this unit, the epMotion can perform a fully automated nucleic acid purification.
2.3.7 CleanCap
As an option, the epMotion can be equipped with a CleanCap.
23
The CleanCap contains a UV lamp and a HEPA filter.
Additional information on your epMotion is available at: www.eppendorf.com/automation
.
24
Product description
®
epMotion
5075
English (EN)
Safety
®
epMotion
English (EN)
5075
3Safety
3.1 Intended use
3.1.1 Intended use of the epMotion 5075
The device is intended for use in laboratories for research, development, and industrial and routine work,
as well as for training purposes. The areas of applications include – but are not limited to – the fields of life
sciences, biotechnology, chemistry and clinical research.
All epMotion 5075 (l, t, v, vt, m) pipetting systems are designed for the monitoring of contamination-free,
precise and correct dispensing and transferring of liquids and to automatically check the combining of
liquids.
The autoclavable dispensing tools work in a volume range from 1 L to 1000 L.
The epMotion5075v with integrated vacuum station, for instance, performs fully automated nucleic acid
purification.
25
The epMotion 5075m with an integrated thermomixer is used for applications which allow mixing and
incubating with open tubes and it performs fully automated nucleic acid purification with magnetic
particles.
The epMotion 5075t with an integrated thermomixer is used for applications which allow mixing and
incubating with open tubes.
The device meets the basic, essential requirements of the EC Directives and Standards that are listed in the
declaration of conformity, but the product is not registered with the FDA.
These automated pipetting systems are exclusively intended for use indoors and may only be used by
skilled personnel who have received adequate training.
3.2 User profile
This device may only be operated by skilled personnel.
The skilled personnel must have received training on this device. The training must have been conducted
by Eppendorf AG or an authorized partner of Eppendorf AG.
The skilled personnel must have read the operating manual carefully. The skilled personnel must have read
the operating manuals of all software components carefully.
26
Safety
epMotion
English (EN)
®
5075
3.3 Information on product liability
In the following cases, the designated protection of the device may be compromised. Liability for any
resulting property damage or personal injury is then transferred to the operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables which are not recommended by Eppendorf.
• The device is maintained or repaired by people not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
3.4 Warnings for intended use
DANGER! Risk of explosion.
Do not operate the device in areas where work is completed with explosive substances.
Do not use this device to process any explosive or highly reactive substances.
Do not use this device to process any substances which may generate an explosive
atmosphere.
DANGER! Damage to health due to escaping aerosols.
When working with substances that can be hazardous to health, harmful aerosols may form
and escape from the vacuum pump.
Contact Eppendorf AG before using this type of substances.
WARNING! Lethal voltages inside the device.
Ensure that the housing is always closed and undamaged so that no parts inside the device
can be contacted by accident.
Do not remove the housing of the device.
Do not allow any liquids to penetrate the inside of the housing.
Do not allow the device to be opened by anyone except service personnel who have been
specifically authorized by Eppendorf.
WARNING! Electric shock due to damage to device or mains cable.
Only switch on the device if the device and mains cable are undamaged.
Only use devices that have been properly installed or repaired.
In case of danger, disconnect the device from the mains supply by pulling the power plug
from the device or the mains socket or, by using the isolating device intended for this
purpose (e.g. emergency stop switch in the laboratory).
Safety
®
epMotion
English (EN)
WARNING! Risk from incorrect supply voltage
5075
Only connect the device to voltage sources which correspond to the electrical
requirements on the name plate.
Only use sockets with a protective earth (PE) conductor and suitable power cable.
WARNING! Damage to health due to infectious liquids and pathogenic germs.
When handling infectious liquids and pathogenic germs, observe the national regulations,
the biological security level of your laboratory, the material safety data sheets, and the
manufacturer's application notes.
Wear personal protective equipment.
For full instructions regarding the handling of germs or biological material of risk group II
or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health
Organization, current edition of the Laboratory Biosafety Manual).
WARNING! Health hazard from skin contact with infectious substances.
27
Wear protective gloves.
WARNING! Danger due to flammable and infectious liquids in the waste container.
Pipette tips in the waste container may contain flammable or infectious liquids.
Wear personal protective equipment.
Handle the pipette tips and sample materials from the waste container in accordance with
the material safety data sheets, safety regulations and laboratory guidelines.
WARNING! Damage to health due to contaminated device and accessories.
Decontaminate the device and the accessories before storage and shipping.
CAUTION! Risk of injury due to carrier movement.
The carrier may still be moving when the front hood of the epMotion is opened.
Wait until the carrier stops moving before reaching into the device.
CAUTION! Cuts due to broken glass.
A damaged touchscreen leads to cuts on the hands.
Only use the MultiCon if it is not damaged.
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf may
impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or
accept any liability for damage resulting from the use of incorrect or non-recommended
accessories and spare parts, or from the improper use of such equipment.
Only use accessories and original spare parts recommended by Eppendorf.
28
Safety
epMotion
English (EN)
®
5075
NOTICE! Spilled liquid can cause damage to the device.
Switch off the device.
Disconnect the power plug.
Collect the spilled liquid. Observe the specifications for the liquid in the material safety
data sheets.
NOTICE! Size of disposables can change through autoclaving.
Do not use autoclaved disposable products in automated applications.
NOTICE! Data loss and malfunction due to malicious software.
Malicious software (e.g., a computer virus) on the USB storage medium can be transferred to
the MultiCon.
Before connecting the USB storage medium to the MultiCon, check the USB storage
medium using a current antivirus software.
NOTICE! Data loss due to missing data backup or incorrect storage of storage media.
User accounts, applications, labware and protocols are saved in a database. If this database
becomes damaged (e.g., due to a hardware defect), this information will be lost.
Eppendorf is not liable for data loss or any associated damages.
Regularly backup your database using the backup function in the software.
Save the secured data on an external storage medium.
Store the external storage media according to the manufacturer’s specifications.
NOTICE! Malfunction due to third-party software.
Third-party software can impair the functioning of the epBlue software.
Only install software approved by Eppendorf.
epMotion
epMotio n U 50 75epMotio n 5 07 5
6
5
1
2
3
4
English (EN)
3.5 Hazard symbols and safety devices on the device
This section explains the warning symbols on the epMotion and the location of the safety devices.
Abb. 3-1: Hazard symbols on the epMotion 5075
Safety
®
5075
29
Fig. 3-1: Hazard symbols on the epMotion 5075
Tab. 3-1: Hazard symbols
1 WARNING
Observe the operating manual.
2 WARNING
The carrier may continue moving after the front panel has
been opened.
Wait until the carrier stops moving before reaching into
the device.
30
Safety
®
epMotion
5075
English (EN)
3DANGER
Risk of burns from the hot surface.
Wait until the surface has cooled before touching it.
5NOTICE
Danger due to UV radiation.
Wear personal protective equipment.
6 The label is located on the magnetic finger module and is
only visible to the service technician.
WARN ING
Danger due to strong magnetic field.
Do not work on the device if you wear a pacemaker.
Tab. 3-2: Safety device
4 The housing protects the user from UV radiation, magnetic
fields, contamination and movable parts.
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