Eppendorf 5075 User Manual

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epMotion

5075

Hardware manual

2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior

permission of the copyright owner.

Trademarks

Eppendorf

, the Epppendorf logo, epMotion®, Eppendorf Tubes ®, and epT.I.P.S.® are registered

trademarks of Eppendorf AG.

LightCycler

and MagNA Pure® are registered trademarks of Roche Diagnostics.

epMotion VAC sealing foil U.S. Pat. No. 6,666,978

epMotion optical sensor U.S. Pat. No. 6,819,437

epMotion gripping tool U.S. Pat. No. 7,462,327

Registered trademarks are not marked in all cases with

or ™ in this manual.

The software of the device (firmware) contains open source software. License information is available on request from Eppendorf AG.

Only for epMotion M5073, M5073c, 5075m and 5075mc: Limited Use Label License NOTICE TO PURCHASER; LIMITED LICENSE FOR RESEARCH USE ONLY.

This product and its use may be covered by one or more patents owned by Gen-Probe Incorporated. The purchase price for this product includes only limited, nontransferable rights under certain claims of certain patents owned by Gen-Probe Incorporated to use this product for research purposes only. No other rights are conveyed. Purchaser is not granted any rights under patents of Gen-Probe Incorporated to use this product for any commercial use. Further information regarding purchasing a license under patents of Gen-Probe Incorporated to use this product for any other purposes, including, without limitation, for commercial use, may be obtained by contacting Gen-Probe Incorporated, Attn: Business Development Department, 10210 Genetic Center Drive, San Diego, California 92121-4362, U.S.A.

5075 900.840-00/062013

Table of contents

epMotion

5075

English (EN)

Table of contents

1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

1.5 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

1.6 Revision history. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

2.1.1 epMotion 5075 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

2.1.2 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

2.1.3 MultiCon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

2.2 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

2.2.1 epMotion 5075l and epMotion 5075lg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

2.2.2 epMotion 5075t and epMotion 5075tg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

2.2.3 epMotion 5075v and epMotion 5075vg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

2.2.4 epMotion 5075vt and epMotion 5075vtg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

2.2.5 epMotion 5075m and epMotion 5075mg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

2.2.6 Accessories for all versions of the epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

2.2.7 Accessories for epMotion with epBlue ID. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

2.2.8 Accessories for epMotion with epBlue GxP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

2.3.1 Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

2.3.2 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

2.3.3 Thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

2.3.4 Thermomixer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

2.3.5 Magnetic separation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

2.3.6 Vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

2.3.7 CleanCap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

3.1.1 Intended use of the epMotion 5075 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

3.5 Hazard symbols and safety devices on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

4.2 Installing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Table of contents

epMotion

5075

English (EN)

5 Hardware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

5.1 Worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

5.1.1 Layout of the epMotion 5075I worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

5.1.2 Layout of the epMotion 5075t worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

5.1.3 Layout of the epMotion 5075v worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

5.1.4 Layout of the epMotion 5075vt worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

5.1.5 Layout of the epMotion 5075m worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

5.2 Optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

5.3 Thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

5.3.1 Safety notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

5.3.2 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

5.3.3 Equipping the thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

5.4 Thermomixer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

5.4.1 Safety notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

5.4.2 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

5.4.3 Equipping the thermomixer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

5.5 Magnet finger module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

5.5.1 Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

5.5.2 Equipping the magnet finger module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

5.6 Vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

5.6.1 Vac Frame vacuum frame . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

5.6.2 VAC Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

5.6.3 Silicone mat for VAC Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

5.6.4 Vac Thermo Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

5.6.5 Elution plate adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

5.6.6 Loading the vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

5.7 CleanCap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

5.7.1 HEPA filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

5.7.2 UV lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

5.8 Barcode reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

5.9 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

5.9.1 Dispensing Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

5.9.2 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

6 Dispensing liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

6.1 Basic procedure for dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

6.1.1 Operating principle of dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

6.1.2 Liquid aspiration up to the remaining volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

6.1.3 Bottom tolerance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

6.1.4 Transporting and dispensing liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

6.2 Dispensing modes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

6.2.1 Dispensing mode: Pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

6.2.2 Dispensing mode: Multidispense . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

6.2.3 Mixing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

6.3 Special features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

6.3.1 Liquid aspiration from bottom of vessel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

6.3.2 Liquid aspiration from high vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

6.3.3 Liquid dispensing at the height of the vessel edge. . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

6.3.4 Liquid aspiration from a filter plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Table of contents

epMotion

5075

English (EN)

7 Labware – vessels, plates and pipette tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

7.1 Tubes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

7.2 Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

7.2.1 Deepwell plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

7.2.2 Microplates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

7.2.3 Filter plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

7.2.4 Channeling plate 96 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

7.2.5 PCR plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

7.2.6 Tube plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

7.3 Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

7.4 epT.I.P.S. Motion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

7.4.1 epT.I.P.S. Motion Racks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

7.4.2 epT.I.P.S. Motion Reloads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

7.4.3 Tip holder for epT.I.P.S. Motion Reloads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

7.4.4 epT.I.P.S. Motion SafeRacks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

8 Labware accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

8.1 Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

8.1.1 Racks for 24 vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

8.1.2 Rack for 96 vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

8.1.3 Rack LC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

8.1.4 Thermorack and Rack 0.5/1.5/2.0 mL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

8.1.5 PrepRack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

8.1.6 ReagentRack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

8.1.7 Reservoir Rack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

8.1.8 ReservoirRack 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

8.1.9 Reservoir Rack modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

8.2 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

8.2.1 Height adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

8.2.2 Vac holder vacuum holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

8.2.3 Thermoadapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

8.2.4 Thermoadapter LC samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

8.2.5 Thermoadapter Frosty. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

8.3 Thermoblocks and Thermoadapters for PCR plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

8.3.1 Equipping the thermoblock with 0.2 mL vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

8.4 Waste container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

8.4.1 Waste container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

8.4.2 Waste funnel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

8.4.3 LiquidWasteTub with lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

Table of contents

epMotion

5075

English (EN)

9 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

9.1 First steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

9.1.1 Switching on the epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

9.1.2 Using the MultiCon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

9.1.3 Using the epMotion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

9.2 Loading the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

9.2.1 Display the loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

9.2.2 Positioning tools on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

9.2.3 Positioning the epT.I.P.S. Motion on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

9.2.4 Positioning the waste container on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

9.2.5 Positioning labware on the worktable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

9.3 Executing an application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

9.3.1 Performing a test run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

9.3.2 Starting the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

9.3.3 Controlling the application run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

9.4 Completing your work. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

9.4.1 Cleaning up the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

9.4.2 Switching off the epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

10 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97

10.1 Decontamination and cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97

10.1.1 Carrying out wipe decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

10.1.2 Carrying out spray decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

10.1.3 Carrying out UV irradiation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

10.1.4 Autoclaving . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

10.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

10.3 Servicing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

10.4 Servicing the vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

10.4.1 Replacing the seals of the vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

10.4.2 Replacing the silicone mat of the vacuum lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

10.5 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

10.6 Carrying out firmware updates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

10.7 Checking the dispensing volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

10.8 Maintaining the dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103

10.8.1 Exchanging the sealing rings on the eight-channel dispensing tool . . . . . . . . . . . . . 103

10.9 Customer service functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104

11 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105

11.1 Error search . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105

11.2 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106

11.2.1 Optical sensor errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106

11.2.2 Dispensing errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

11.2.3 Software errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

12 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109

12.1 Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109

12.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109

12.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110

Table of contents

epMotion

5075

English (EN)

13 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

13.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

13.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

13.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

13.4 Noise level. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

13.5 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

13.6 MultiCon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

13.7 Additional specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

13.7.1 Optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

13.7.2 Carrier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

13.7.3 Gripper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

13.8 Enhancements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113

13.8.1 Thermal module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113

13.8.2 Thermomixer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113

13.8.3 Magnet finger module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113

13.8.4 Vacuum unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113

13.8.5 Barcode reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114

13.8.6 HEPA filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114

13.8.7 UV lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114

13.9 Dispensing tool errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115

13.9.1 Pipetting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115

13.9.2 Dispensing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116

13.10 Test conditions for dispensing tool errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116

14 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117

14.1 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117

14.2 epT.I.P.S. Motion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117

14.3 Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119

14.4 Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119

14.5 Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120

14.6 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121

14.7 Thermoblocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121

14.8 Accessories for the vacuum chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122

14.9 MagSep kits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122

14.10 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122

14.11 Modification kits and enhancements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124

Table of contents

epMotion

English (EN)

5075

Operating instructions

epMotion

English (EN)

1 Operating instructions

1.1 Using this manual

Your epMotion operating manual consists of hardware instructions and software instructions. Short instructions are available for optional software enhancements.

The operating manual is part of the product.

5075

The current version of the operating manual can be found on our webpage: www.eppendorf.com

 Read the operating manual in full before using the device.

 Store the operating manual at an easily accessible location.

 The device may only be transferred with the operating manual.

 If the operating manual is lost, replace it immediately. Please contact Eppendorf AG for further details.

1.2 Danger symbols and danger levels

The safety instructions in this manual appear with the following danger symbols and danger levels:

1.2.1 Danger symbols

Biohazard Explosion

Strong magnetic field UV radiation

Electric shock Heavy loads

Cuts Hot surface

Hazard point Material damage

1.2.2 Danger levels

DANGER Will lead to severe injuries or death.

WARN ING May lead to severe injuries or death.

CAUTION May lead to light to moderate injuries. NOTICE May lead to material damage.

Operating instructions

epMotion English (EN)

5075

1.3 Symbols used

Depiction Meaning

Actions in the specified order

 Actions without a specified order

• List:

Text Display or software texts

Additional information

1.4 Abbreviations used

BCR

Barcode reader

DNA

Deoxyribonucleic acid (DNA)

DWP

Deepwell plate

epT.I.P.S. eppendorf Totally Integrated Pipetting System

HEPA

High-Efficiency Particulate Air (filter)

Liquid handling

MTP

Micro test plate

PCR

Polymerase chain reaction

PDF

Portable Document Format

TMX

Thermomixer

USB

Universal Serial Bus

Ultraviolet radiation

VAC

Vacuum unit

Operating instructions

epMotion

English (EN)

1.5 Glossary

Application

Programs for a specific application. An application includes the procedure and the equipping of the worktable.

Barcode reader

Device for scanning a barcode.

CleanCap

Optional epMotion equipment. The CleanCap includes a HEPA filter and UV lamp.

5075

Destination labware

Labware that liquid is dispensed into during the application. Destination labware refers to a plate or rack.

Destination position

Position that liquid is dispensed into during the application.

Destination vessel

The vessel into which liquid is dispensed during the application.

Dispensing tool

Tool that aspirates and dispenses liquid. Single-channel dispensing tools and eight-channel dispensing tools are available for various volume ranges.

epBlue

Software for creating and administering applications and labware. The epMotion is controlled using the software. epBlue is used to manage access to epMotion. epBlue can be expanded. The ID module enables barcode tracking. The GxP module enables electronic documentation in support of 21 CFR Part 11.

Eppendorf Quality

Eppendorf Quality is an Eppendorf AG purity grade for consumables. Eppendorf Quality meets the requirements for standard products, e.g., precision, accuracy, wetting behavior and tightness.

epT.I.P.S.® Motion

epMotion pipette tips. Only epT.I.P.S.® Motion can be used on the epMotion for all features. epT.I.P.S.® Motions are available with or without a filter.

Operating instructions

epMotion

5075

English (EN)

Filling volume

Maximum liquid volume of a labware. The epMotion uses the gripper to transport the labware up to the filling volume. The epMotion aspirates liquid from the labware up to the filling volume. The filling volume is higher than the working volume.

Gripper

Tool that transports labware.

Height adapter

Adapter for low labware. The height differences among the labware will be offset to decrease the tool holder paths, thereby decreasing the run time of the application as well.

HEPA filter

Air filter. The air filter prevents particles such as dust and germs from the environment entering the epMotion work room.

Intermediate labware

Labware that is used to dispense and aspirate liquids during the application.

Intermediate position

Position that is used to dispense and aspirate liquids during the application.

Intermediate vessel

Vessel that is used to dispense and aspirate liquids during the application.

Labware

Racks, plates, tips, etc. which are placed on the worktable.

Location

Area on the worktable where the labware is placed.

MagSep

System consisting of the magnetic finger module, thermomixer and PrepRack and reagents for nucleic acid preparation with the epMotion.

MultiCon

epMotion operator panel. You can control your epMotion using the control unit and the epBlue software.

Operating instructions

epMotion

English (EN)

PCR clean

PCR clean is an Eppendorf AG purity grade for consumables. PCR clean meets the requirements for standard products, e.g., precision, accuracy, wetting behavior, tightness. PCR clean also meets the requirements with regard to absence of human DNA, DNase, RNase and PCR inhibitors. Consumables with the PCR clean purity grade are controlled and certified by an external laboratory. Certificates are available for downloading from our webpage www.eppendorf.com

Procedure

Sequence of commands that are executed one after the other. Part of an application.

Rack

Mount for tubes or pipette tips.

5075

Random error

Precision. Standard deviation of the average value of the dispensed volumes.

Remaining volume

Volume that cannot be aspirated from a vessel. The distance from the pipette tip to the vessel bottom, defined in the software, and the defined immersion depth of the pipette tip in the liquid, must be observed. Therefore, the pipette tip cannot aspirate the volume. The remaining volume depends on the vessel geometry.

Reservoir

Reservoirs are used to hold reagents. Reservoirs are hung in a Reservoir Rack or placed directly on the worktable.

SafeRack

Rack with ep.T.I.P.S. Motion pipette tips. The SafeRack features a partition that prevents the contamination of adjacent tips. Use the SafeRacks if you would like to use tips several times.

Source labware

Labware that liquid is aspirated from during the application. Source labware refers to a plate or rack.

Source position

Position that liquid is aspirated from during the application.

Source vessel

The vessel from which liquid is aspirated during the application.

Sterile

Sterile is an Eppendorf AG purity grade for consumables. Sterile meets the requirements for standard products, e.g., precision, accuracy, wetting behavior, tightness. Sterile also meets the requirements with regard to sterility and freedom from pyrogens.

Operating instructions

epMotion

5075

English (EN)

Systematic error

Accuracy. Deviation of the average value of the dispensed volumes from the selected volume.

Thermal module

Thermal module for labware that is integrated in the worktable.

Thermoadapter

Heat-conductive adapter for holding plates.

Thermoblock

A thermoadapter that is permanently connected to a PCR plate or PCR tube.

Thermorack

Temperable rack for smaller vessels, e.g., Safe-Lock tubes for 0.5 mL, 1.5 mL or 2 mL.

Vacuum unit

The vacuum unit uses a vacuum to suck liquid from a filter plate. The vacuum unit consists of a vacuum manifold, vacuum pump and Vac Frame.

Vessel

Tube or single well of a plate.

Work volume

Liquid volume of a labware. The epMotion fills a vessel with low levels of contamination up to the working volume. The working volume is less than the filling volume.

Worktable

Work surface of the epMotion where labware and tools are placed. In the software, the epMotion worktable is shown as the epBlue worktable.

Operating instructions

1.6 Revision history

Revision history of the epMotion 5075 hardware operating manual, order no. 5075 900.840.

Version of the operating manual Date Changes

Version 00 June 2013 Created new

epMotion

English (EN)

5075

Operating instructions

epMotion

5075

English (EN)

Product description

epMotion

English (EN)

2 Product description

2.1 Main illustration

The epMotion 5075 is available with various features. Details of the worktable for your device (see Worktable on p. 33).

2.1.1 epMotion 5075

Abb. 2-1: Main illustrati on epMotion 5075I

5075

Fig. 2-1: Main illustration epMotion 5075I

1Carrier

The carrier moves in X-direction, Y-direction and Z-direction.

2 Tool holder

Holds dispensing tools.

epMotio n 5 07 5

6 Front hood

Safety device for protection from movable, UV radiation parts and contamination.

7 Thermal module (optional)

Plate for heating and cooling labware.

3Optical sensor

Detects levels, tips and labware.

4CleanCap (optional)

contains HEPA filter and UV lamps

5Worktable

Work surface for tools and labware.

8 Mains power switch

9Mouse

10 MultiCon

Control unit used to control the epMotion.

Product description

epMotion

5075

English (EN)

2.1.2 Interfaces

Abb. 2-2: Interfaces epMoti on 5075

Fig. 2-2: Interfaces epMotion 5075

1Ethernet

3 Mains power connection

Connection for the cable to the MultiCon

2USB

Connection for a USB storage medium for firmware updates.

Only devices which meet the requirements of IEC 950/EN 60950-1 (UL 1950) standards may be connected to the interfaces.

2.1.3 MultiCon

Information can be found in the operating manual of the MultiCon.

Only devices which meet the requirements of IEC 950/EN 60950-1 (UL 1950) standards may be connected to the interfaces.

Product description

epMotion

English (EN)

2.2 Delivery package

The epMotion is available in various versions. Your device has been equipped according to your specifications. For some software modules additional accessories are available.

2.2.1 epMotion 5075l and epMotion 5075lg

5075

Quantity Order no.

(International)

1 5075 000.271 100 V - 240 V, 50 / 60 Hz

or 1 5075 000.275 100 V - 240 V, 50 / 60 Hz

Description

Automated pipetting system epMotion 5075l

main device; with MultiCon, keyboard, mouse,  installed software epBlue

Automated pipetting system epMotion 5075lg

main device; with MultiCon, keyboard, mouse,  installed software epBlue GxP

2.2.2 epMotion 5075t and epMotion 5075tg

Quantity Order no.

(International)

1 5075 000.272 100 V - 240 V, 50 / 60 Hz

or 1 5075 000.276 100 V - 240 V, 50 / 60 Hz

Description

Automated pipetting system epMotion 5075t

main device with thermomixer; with MultiCon, keyboard, mouse, installed software epBlue

Automated pipetting system epMotion 5075tg

main device with thermomixer; with MultiCon, keyboard, mouse, installed software epBlue GxP

2.2.3 epMotion 5075v and epMotion 5075vg

Quantity Order no.

(International)

1 5075 000.273 100 V - 240 V, 50 / 60 Hz

or 1 5075 000.277 100 V - 240 V, 50 / 60 Hz 1– Reservoir 400 mL

1 5075 785.005 vacuum frame

1 5075 778.009 adapter for Vac Frame 1 5282 000.018 Gripper

Description

Automated pipetting system epMotion 5075v

main device with vacuum unit; with MultiCon, keyboard, mouse, installed software epBlue

Automated pipetting system epMotion 5075vg

main device with vacuum unit; with MultiCon, keyboard, mouse, installed software epBlue GxP

Vac Frame 2

Vac Holder

Product description

epMotion English (EN)

5075

2.2.4 epMotion 5075vt and epMotion 5075vtg

Quantity Order no.

(International)

1 5075 000.274 100 V - 240 V, 50 / 60 Hz

or 1 5075 000.278 100 V - 240 V, 50 / 60 Hz 1– Reservoir 400 mL

1 5075 785.005 vacuum frame

1 5075 778.009 adapter for Vac Frame 1 5282 000.018 Gripper

Description

Automated pipetting system epMotion 5075vt

main device with vacuum unit and thermomixer; with MultiCon, keyboard, mouse, installed software epBlue

Automated pipetting system epMotion 5075vtg

main device with vacuum item and thermomixer; with MultiCon, keyboard, mouse, installed software epBlue GxP

Vac Frame 2

Vac Holder

2.2.5 epMotion 5075m and epMotion 5075mg

Quantity Order no.

(International)

1 5075 000.270 100 V - 240 V, 50 / 60 Hz

or 1 5075 000.279 100 V - 240 V, 50 / 60 Hz

Description

Automated pipetting system epMotion 5075m

main device with magnet finger module and thermomixer; with MultiCon, keyboard, mouse, installed software epBlue

Automated pipetting system epMotion 5075mg

main device with magnet finger module and thermomixer; with MultiCon, keyboard, mouse, installed software epBlue GxP

2.2.6 Accessories for all versions of the epMotion

Product description

epMotion

5075

English (EN)

Quantity Order no.

(International)

1 - With USB connection cable



1 - Compatible with the country where the order was placed or defined

1 - For connecting the MultiCon to a network 1- Tool for transport securing device 1 5075 753.006 Waste box

1 5075 900.840 epMotion 5075 1 5073 900.866 software operating manual epBlue with MultiCon

optional 1 5075 751.607 with HEPA air filter and UV-lamp



optional 2-



Description

Mouse

Keyboard

Cable

For connecting the MultiCon to the epMotion

Mains power cable

USB-Ethernet adapter

Operating Manual

CleanCap

UV tube

For epMotion with CleanCap

2.2.7 Accessories for epMotion with epBlue ID

Quantity Order no.

(International)



–

 Barcode reader

Description

USB dongle eLicenser

with hardware key code card

With USB connection cable and table stand

2.2.8 Accessories for epMotion with epBlue GxP

Quantity Order no.

(International)



1 5075 900.874 epBlue GxP



–



–

Description

USB dongle eLicenser

with hardware key code card

Documentation folder

Folder for certificates and documentation

Operating Manual

Product description

epMotion English (EN)

5075

2.3 Features

The epMotion 5075 is available with different features. All available features are described in this manual.

2.3.1 Function

The epMotion 5075 is an automatic system for dispensing liquids using dispensing tools and ep.T.I.P.S. Motion pipette tips.

The epMotion 5075 dispenses liquids in the volume range 1 µL – 1 000 µL.

The dispensing tool aspirates liquid from source vessels and dispenses this liquid in destination vessels. The dispensing tool works according to the piston-stroke principle. For the epMotion, dispensing tools are available for 3 different volume ranges.

The epMotion features an optical sensor. The sensor checks:

• Type and location of labware

• Quantity and position of pipette tips in the rack

• Filling level of vessels

You control the epMotion 5075 via the MultiCon control unit with the epBlue software.

You can use the epBlue software to define dispensing processes and compile them into an application. To do so, you select source vessels and destination vessels, define the procedure and define the transfer pattern.

2.3.2 Gripper

A gripper is available for the epMotion. The gripper stacks and transports labware.

2.3.3 Thermal module

The epMotion can be equipped with a maximum of 3 thermal modules.

2.3.4 Thermomixer

The epMotion can be equipped with a thermomixer.

Product description

epMotion

5075

English (EN)

2.3.5 Magnetic separation

The epMotion can be equipped with a thermomixer and a magnet finger module for separating magnetic particles.

This epMotion is a system for preparing nucleic acids. MagSep reagent kits are available for this system.

2.3.6 Vacuum unit

The epMotion can be equipped with a vacuum unit.

With this unit, the epMotion can perform a fully automated nucleic acid purification.

2.3.7 CleanCap

As an option, the epMotion can be equipped with a CleanCap.

The CleanCap contains a UV lamp and a HEPA filter.

Additional information on your epMotion is available at: www.eppendorf.com/automation

Product description

epMotion

5075

English (EN)

Safety

epMotion

English (EN)

5075

3Safety

3.1 Intended use

3.1.1 Intended use of the epMotion 5075

The device is intended for use in laboratories for research, development, and industrial and routine work, as well as for training purposes. The areas of applications include – but are not limited to – the fields of life sciences, biotechnology, chemistry and clinical research.

All epMotion 5075 (l, t, v, vt, m) pipetting systems are designed for the monitoring of contamination-free, precise and correct dispensing and transferring of liquids and to automatically check the combining of liquids.

The autoclavable dispensing tools work in a volume range from 1 L to 1000 L.

The epMotion5075v with integrated vacuum station, for instance, performs fully automated nucleic acid purification.

The epMotion 5075m with an integrated thermomixer is used for applications which allow mixing and incubating with open tubes and it performs fully automated nucleic acid purification with magnetic particles.

The epMotion 5075t with an integrated thermomixer is used for applications which allow mixing and incubating with open tubes.

The device meets the basic, essential requirements of the EC Directives and Standards that are listed in the declaration of conformity, but the product is not registered with the FDA.

These automated pipetting systems are exclusively intended for use indoors and may only be used by skilled personnel who have received adequate training.

3.2 User profile

This device may only be operated by skilled personnel.

The skilled personnel must have received training on this device. The training must have been conducted by Eppendorf AG or an authorized partner of Eppendorf AG.

The skilled personnel must have read the operating manual carefully. The skilled personnel must have read the operating manuals of all software components carefully.

Safety

epMotion English (EN)

5075

3.3 Information on product liability

In the following cases, the designated protection of the device may be compromised. Liability for any resulting property damage or personal injury is then transferred to the operator:

• The device is not used in accordance with the operating manual.

• The device is used outside of its intended use.

• The device is used with accessories or consumables which are not recommended by Eppendorf.

• The device is maintained or repaired by people not authorized by Eppendorf.

• The user makes unauthorized changes to the device.

3.4 Warnings for intended use

DANGER! Risk of explosion.

 Do not operate the device in areas where work is completed with explosive substances.  Do not use this device to process any explosive or highly reactive substances.  Do not use this device to process any substances which may generate an explosive

atmosphere.

DANGER! Damage to health due to escaping aerosols.

When working with substances that can be hazardous to health, harmful aerosols may form and escape from the vacuum pump.

 Contact Eppendorf AG before using this type of substances.

WARNING! Lethal voltages inside the device.

 Ensure that the housing is always closed and undamaged so that no parts inside the device

can be contacted by accident.

 Do not remove the housing of the device.  Do not allow any liquids to penetrate the inside of the housing.  Do not allow the device to be opened by anyone except service personnel who have been

specifically authorized by Eppendorf.

WARNING! Electric shock due to damage to device or mains cable.

 Only switch on the device if the device and mains cable are undamaged.  Only use devices that have been properly installed or repaired.  In case of danger, disconnect the device from the mains supply by pulling the power plug

from the device or the mains socket or, by using the isolating device intended for this purpose (e.g. emergency stop switch in the laboratory).

Safety

epMotion

English (EN)

WARNING! Risk from incorrect supply voltage

5075

 Only connect the device to voltage sources which correspond to the electrical

requirements on the name plate.

 Only use sockets with a protective earth (PE) conductor and suitable power cable.

WARNING! Damage to health due to infectious liquids and pathogenic germs.

 When handling infectious liquids and pathogenic germs, observe the national regulations,

the biological security level of your laboratory, the material safety data sheets, and the manufacturer's application notes.

 Wear personal protective equipment.  For full instructions regarding the handling of germs or biological material of risk group II

or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health Organization, current edition of the Laboratory Biosafety Manual).

WARNING! Health hazard from skin contact with infectious substances.

 Wear protective gloves.

WARNING! Danger due to flammable and infectious liquids in the waste container.

Pipette tips in the waste container may contain flammable or infectious liquids.

 Wear personal protective equipment.  Handle the pipette tips and sample materials from the waste container in accordance with

the material safety data sheets, safety regulations and laboratory guidelines.

WARNING! Damage to health due to contaminated device and accessories.

 Decontaminate the device and the accessories before storage and shipping.

CAUTION! Risk of injury due to carrier movement.

The carrier may still be moving when the front hood of the epMotion is opened.

 Wait until the carrier stops moving before reaching into the device.

CAUTION! Cuts due to broken glass.

A damaged touchscreen leads to cuts on the hands.

 Only use the MultiCon if it is not damaged.

CAUTION! Poor safety due to incorrect accessories and spare parts.

The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non-recommended accessories and spare parts, or from the improper use of such equipment.

 Only use accessories and original spare parts recommended by Eppendorf.

Safety

epMotion English (EN)

5075

NOTICE! Spilled liquid can cause damage to the device.

 Switch off the device.  Disconnect the power plug.  Collect the spilled liquid. Observe the specifications for the liquid in the material safety

data sheets.

NOTICE! Size of disposables can change through autoclaving.

 Do not use autoclaved disposable products in automated applications.

NOTICE! Data loss and malfunction due to malicious software.

Malicious software (e.g., a computer virus) on the USB storage medium can be transferred to the MultiCon.

 Before connecting the USB storage medium to the MultiCon, check the USB storage

medium using a current antivirus software.

NOTICE! Data loss due to missing data backup or incorrect storage of storage media.

User accounts, applications, labware and protocols are saved in a database. If this database becomes damaged (e.g., due to a hardware defect), this information will be lost. Eppendorf is not liable for data loss or any associated damages.

 Regularly backup your database using the backup function in the software.  Save the secured data on an external storage medium.  Store the external storage media according to the manufacturer’s specifications.

NOTICE! Malfunction due to third-party software.

Third-party software can impair the functioning of the epBlue software.

 Only install software approved by Eppendorf.

epMotion

epMotio n U 50 75epMotio n 5 07 5

English (EN)

3.5 Hazard symbols and safety devices on the device

This section explains the warning symbols on the epMotion and the location of the safety devices.

Abb. 3-1: Hazard symbols on the epMotion 5075

Safety

5075

Fig. 3-1: Hazard symbols on the epMotion 5075

Tab. 3-1: Hazard symbols

1 WARNING

Observe the operating manual.

2 WARNING

The carrier may continue moving after the front panel has been opened.

 Wait until the carrier stops moving before reaching into

the device.

Safety

epMotion

5075

English (EN)

3DANGER

Risk of burns from the hot surface.

 Wait until the surface has cooled before touching it.

5NOTICE

Danger due to UV radiation.

 Wear personal protective equipment.

6 The label is located on the magnetic finger module and is

only visible to the service technician.

WARN ING

Danger due to strong magnetic field.

 Do not work on the device if you wear a pacemaker.

Tab. 3-2: Safety device

4 The housing protects the user from UV radiation, magnetic

fields, contamination and movable parts.

Installation

epMotion

English (EN)

5075

4 Installation

4.1 Selecting the location

Information on ambient conditions, dimensions and weights can be found in the technical data (see Weight/ dimensions on p. 111).

Select the location for the device according to the following criteria:

Electrical connections

• Mains connection in accordance with the name plate.

• The device mains/power switch and cutting unit of the power system circuit (e.g., FI protective switch) can be accessed during operation.

Location

• The ambient conditions match the specifications in the technical data.

• The location is well ventilated.

• The location is protected from direct sunlight.

• The location is not next to heat sources, such as heaters or drier compartments.

• There is an adequate amount of space available for the device. The minimum distances to other devices and walls is 6 cm.

• The device can be safely and easily operated at this location.

Workplace

• The lab bench is permanently mounted.

• The lab bench is designed for the weight of the device.

• The lab bench has a horizontal, plane work surface.

• The lab bench has a non-slip surface.

• The lab bench is vibration-free.

• There are no vibrating devices on the lab bench.

4.2 Installing the device

The epMotion may only be installed and commissioned by skilled personnel authorized by Eppendorf.

Information on transport (see Transport on p. 109).

Installation

epMotion

English (EN)

5075

5Hardware

TM 1

TM 2

TM 3

Waste

T0 T1

A3 A4 A5

B3 B4 B5B2B1B0

C1 C2 C3 C4 C5

TM 1

TM 2

TMX

Waste

T0 T1

A4 A5

B3 B4 B5B2B1B0

C1 C2 C3 C4 C5

5.1 Worktable

5.1.1 Layout of the epMotion 5075I worktable

Abb. 5-1: Worktable for epMotion 5075I

Hardware

epMotion

English (EN)

5075

Fig. 5-1: Worktable for epMotion 5075I

A2-C5

Locations for labware

C1-C3

Waste

With up to 3 thermal modules as an optional feature

T1-T4

Locations for dispensing tools

5.1.2 Layout of the epMotion 5075t worktable

Abb. 5-2: Worktable for epMotion 5075t

With a gripper as an optional feature

Location for waste container

Fig. 5-2: Worktable for epMotion 5075t

A2-C5

Locations for labware

C1-C2

With 2 thermal modules as an optional feature

Thermomixer

T1-T4

Locations for dispensing tools

With a gripper as an optional feature

Waste

Location for waste container

TM 1

TM 2

TM 3

VAC

Waste

T0 T1

A3 A4

B3B2B1B0

C1 C2 C3 C4

TM 1

TM 2

TMX

Waste

T0 T1

B3B2B1B0

C1 C2 C3 C4

VAC

Hardware

epMotion

5075

English (EN)

5.1.3 Layout of the epMotion 5075v worktable

Abb. 5-3: Worktable for epMotion 5075v

Fig. 5-3: Worktable for epMotion 5075v

A2-C5

T1-T4

Locations for labware

C1-C3

With 3 thermal modules as an optional feature

Waste

Vacuum unit

5.1.4 Layout of the epMotion 5075vt worktable

Abb. 5-4: Worktable for epMotion 5075vt

Fig. 5-4: Worktable for epMotion 5075vt

Locations for dispensing tools

Location for gripper

Location for waste container

A2-C5

Locations for labware

C1-C2

With up to 2 thermal modules as an optional feature

Thermomixer

Vacuum unit

T1-T4

Locations for dispensing tools

Location for gripper

Waste

Location for waste container

5.1.5 Layout of the epMotion 5075m worktable

TM 1

TM 2

Waste

T0 T1

A4 A5

B3 B4 B5B2B1B0

C1 C2 C3 C4 C5

TMX

MagSep

Abb. 5-5: Worktable for epMotion 5075m

Fig. 5-5: Worktable for epMotion 5075m

Hardware

epMotion

English (EN)

5075

A2-C5

Locations for labware

C1-C2

With up to 2 thermal modules as an optional feature

Thermomixer

MagSep

Magnet finger module

T1-T4

Locations for dispensing tools

With a gripper as an optional feature

Waste

Location for waste container

Detector Diaphragm

Imaging optics

Light source

Semi-transparent mirror

Diaphragm

Hardware

epMotion

5075

English (EN)

5.2 Optical sensor

Abb. 5-6: Optical sensor principle

Fig. 5-6: Optical sensor principle

On the carrier, the optical sensor is located to the right of the tool holder. The optical sensor records the intensity of the reflected light using a lateral infrared light source,

semi-transparent mirror, fixed lens and the procedure in z-direction. Height z

is defined as the point with

the highest intensity which enables the detection of the level in a vessel or the presence of labware. The optical sensor can detect horizontal, plane surfaces. The surface of the liquid must be at an angle of

90° ± 3° to the optical axis.

The surfaces of liquids can be strongly bent by the vessel geometry or the physical properties of the liquid or vessel. The optical sensor may not detect the level on strongly bent surfaces. If this is the case, the user must enter the liquid volume.

The optical sensor has a detection limit for vessel levels. The detection limit depends on the vessel geometry. Generally, levels of 3 mm or higher can be detected.

The following functions of the optical sensor can be activated:

• Liquid detection Determines the filling level for positions of labware, which has the Liquid detection option activated.

• Tip detection Checks to see if the pipette tips defined in the application are available. Determines the quantity and position of tips in the rack. Works only with epT.I.P.S. Motion in TipHolders and Racks from Eppendorf.

• Location detection Detects the labware coding. Checks to see if the labware on the epMotion worktable matches the labware on the epBlue worktable.

Information on setting the optical sensor, and a detailed description of all functions, can be found in the software operating manual.

5.3 Thermal module

Based on the model, your epMotion can be equipped with one or more thermal modules.

5.3.1 Safety notes

WARNING! Danger due to flammable or explosive liquids.

 Do not use explosive substances.  Do not heat any highly flammable substances.  Heat highly flammable substances in small quantities below boiling point only.  Do not exceed the boiling point of solutions.

CAUTION! Burns from hot metal surfaces.

The metal surface heats up during operation. The software displays the current temperature of the metal surface.

Hardware

epMotion

English (EN)

5075

 Wait until the metal surface has cooled down completely.

CAUTION! Burns from hot labware.

Labware will be tempered during operation.

 Do not touch temperature-controlled labware.  Wait until the labware has cooled down completely.

NOTICE! Material damage due to non-heat-resistant labware.

Samples and labware will become damaged if you do not use heat-resistant labware.

 Observe the labware specifications.  At set temperatures in excess of 100 °C, only use labware made of metal, polypropylene

(PP) or polycarbonate (PC).

5.3.2 Configuration

Abb. 5-7: Thermal module

Fig. 5-7: Thermal module

Thermal modules temper liquids in a temperature range of 0 °C – 110 °C.

Hardware

epMotion

5075

English (EN)

Tab. 5-1: Temperature control duration for labware with thermal module

Labware on the  thermal module

Water filling volume per well

Cooling from  25°C to 4°C

Heating from  25°C to 37°C

in µL

Temperature setting in °C

Thermal module 

min Temperature

setting in °C

528

without labware

Thermoadapter with

50 3 7 38 5 104 11 96-well twin.tec  PCR plate skirted

Thermoblock with 96-well

50 3 6 37 4 101 10 twin.tec PCR plate skirted

Thermoadapter with

10 3 5 37 5 103 8 384-well twin.tec  PCR plate skirted

Thermoblock with

10 3 5 37 5 98 7 384-well twin.tec  PCR plate skirted

24-unit thermorack with

350 3 24 37 16 101 35

0.5 mL Safe-Lock tubes

24-unit thermorack with

1200 3 30 38 18 104 44

1.5 mL Safe-Lock tubes

24-unit thermorack with

1700 3 25 37 17 100 40

2.0 mL Safe-Lock tubes

Heating from  25°C to 95°C

min Temperature

setting in °C

min

This information applies at an ambient temperature of 25 °C for the border positions in the labware. The thermal modules were tempered at the target temperature before the labware was positioned.

Information on setting the thermal module and a detailed description of all functions can be found in the "Operation" chapter of the software instructions.

5.3.3 Equipping the thermal module

When you use a thermoblock or thermoadapter, plates with uneven bases will be optimally tempered, e.g., PCR plates.

Equip the thermal module as follows:

1. Place the plate on the thermoadapter or thermoblock.

2. Place the plate, with thermoadapter or thermoblock, on the thermal module.

5.4 Thermomixer

Based on the model, your epMotion can be equipped with a thermomixer.

5.4.1 Safety notes

WARNING! Danger due to flammable or explosive liquids.

CAUTION! Burns from hot metal surfaces.

The metal surface heats up during operation. The software displays the current temperature of the metal surface.

Hardware

epMotion

English (EN)

5075

 Wait until the metal surface has cooled down completely.

CAUTION! Burns from hot labware.

Labware will be tempered during operation.

 Do not touch temperature-controlled labware.  Wait until the labware has cooled down completely.

NOTICE! Contamination of samples and the device due to high mixing frequency.

If the speed is set too high, liquid will spray out of the vessels. Labware may become loose from the holder and fly around.

 Observe the maximum speeds of the labware in the operating instructions.

5.4.2 Configuration

Abb. 5-8: Thermomixer

Fig. 5-8: Thermomixer

The thermomixer tempers and mixes liquids and suspensions.

Hardware

epMotion

5075

English (EN)

The thermomixer works in a temperature range of 4 °C – 95 °C. The lowest temperature the thermomixer reaches is 15 °C below ambient temperature. If the ambient temperature is 20 °C, the thermomixer reaches at least 5 °C.

Tab. 5-2: Maximum speed for labware on the thermomixer

Labware Maximum speed in rpm

Tubes 1000

DWP 96 1200

DWP 384 1200

MTP 6 1000

MTP 96 2000

MTP 384 2000

PCR 96 2000

PCR 384 2000

Reservoir Rack Cannot be mixed

Thermorack/Thermoblock 1000

Thermoadapter 96 1000

Thermoadapter 384 1000

Thermorack TMX 1300

PrepRack 1300

Information on setting the thermomixer, and a detailed description of all functions, can be found in the software instructions.

5.4.3 Equipping the thermomixer

When you use a thermoblock or thermoadapter, plates with uneven bases will be optimally tempered, e.g., PCR plates.

Equip the thermomixer as follows:

1. Place the plate on the thermoadapter or thermoblock.

2. Place the plate, with thermoadapter or thermoblock, on the thermomixer.

The plate will be fastened to the thermomixer during the application.

5.5 Magnet finger module

Based on the model, your epMotion can be equipped with a magnet finger module.

5.5.1 Configuration

Abb. 5-9: Magnet finger module

Hardware

epMotion

English (EN)

5075

Fig. 5-9: Magnet finger module

The magnet finger module is located behind the thermomixer.

The magnet finger module is used to separate suspended magnetic particles.

The magnet finger module has 3 fingers that can be moved horizontally. Strong permanent magnets are located on each finger. The 3 magnet fingers move toward the vessels via the lateral openings in the PrepRack. The magnetic field separates the magnetic particles on the tube inner wall. The dispensing tool can remove liquid from the vessels, or dispenses liquid in the vessels, while the magnetic particles are held in place.

The PrepRack is required to perform a separation (see p. 74).

The separation period can be defined using the epMotion software.

For a full nucleic acid purification, you can use the MagSep kits supplied by Eppendorf AG.

5.5.2 Equipping the magnet finger module

The geometry of the used vessels is important for the quality of the separation. Use 2.0 mL Safe-Lock tubes.

Equip the magnet finger module as follows:

1. Equip the PrepRack with vessels.

2. Place the equipped PrepRack on the thermomixer.

The PrepRack will be fastened on the thermomixer when the application is started.

Hardware

epMotion

5075

English (EN)

5.6 Vacuum unit

Abb. 5-10: Vacuum unit with vacuum frame

Fig. 5-10: Vacuum unit with vacuum frame

The vacuum unit uses a vacuum to suck liquid from a filter plate into a collection vessel. The vacuum is set via the software and generated by a vacuum pump. The vacuum unit consists of a vacuum chamber, vacuum pump and vacuum frame and a lid.

You can use the following labware as collection vessels:

•Micro test plate (MTP)

• Deepwell plate (DWP)

•Tube plate

• 400 mL reservoir

• 300 mL reservoir

The gripper places filter plates, VAC Lid and Vac Thermo Lid on the vacuum frame. The gripper transports the vacuum frame with the attached filter plate.

5.6.1 Vac Frame vacuum frame

Abb. 5-11: VAC Frame 1

Fig. 5-11: VAC Frame 1

The vacuum frame is placed on the vacuum unit. The filter plate is positioned on the vacuum frame.

The frame of the plate must sit on top of the VAC Frame rubber. Otherwise, a vacuum cannot be created. The Vac Frame is available in 2 different heights for this.

The vacuum frame is positioned on the vacuum unit or on the vacuum holder. The vacuum frame cannot be positioned directly on the worktable.

The vacuum frame is transported with the gripper.

5.6.2 VAC Lid

Abb. 5-12: VAC Lid

Fig. 5-12: VAC Lid

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1VAC Lid

3Pins

2Silicone mat

Use the VAC Lid if do not want to apply the vacuum to the entire plate. Place the VAC Lid on the vacuum unit and cover any bores that are not required with the silicone mat. The VAC Lid contains 96 bores. The bores correspond to the positions of the wells on the filter plate.

The VAC Lid is positioned on the vacuum unit or on the gripper holder.

The VAC Lid is transported with the gripper.

5.6.3 Silicone mat for VAC Lid

Abb. 5-13: Bores in the VAC Lid covered with silicone mat

Fig. 5-13: Bores in the VAC Lid covered with silicone mat

If you are not using some of the filter plate wells you can cover the corresponding bores in the VAC Lid with a silicone mat. The air does not flow through the covered bores. The vacuum only affects the wells that are in use.

Silicone mats are available in different sizes. You can cover 16, 32, 48, 64 or 80 bores with the silicone mat. The silicone mat has the number of bores it covers punched into it.

 To attach a silicone mat on the VAC Lid, place the silicone mat on the pins of the VAC Lid.

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5.6.4 Vac Thermo Lid

Abb. 5-14: VAC Thermo Lid

Fig. 5-14: VAC Thermo Lid

The Vac Thermo Lid has a block with a high heat capacity. The Vac Thermo Lid is heated on a thermal module. On the vacuum unit, air is sucked in through the slits in the Vac Thermo Lid. The air heats up on the block and flows through the filter plates.

With the Vac Thermo Lid, you can optimize automated nucleic acid preparation. You can minimize the alcohol in filter plates, the risk of contamination from alcohol is reduced.

The Vac Thermo Lid can be placed on a thermal module and on the vacuum unit.

The gripper transports the Vac Thermo Lid.

The Vac Thermo Lid cannot be placed on the thermomixer.

5.6.4.1 Using the Vac Thermo Lid

CAUTION! Burns from the hot base of the Vac Thermo Lid.

When you temper the Vac Thermo Lid, its base becomes hot.

 Wait until the base of the Vac Thermo Lid has cooled down completely.

1. Place the Vac Thermo Lid on a thermal module.

Do not place a reservoir rack with reservoirs in the transport route of the Vac Thermo Lid. Reservoir racks with reservoirs collide with the VAC Thermo Lid on the gripper.

2. Heat the Vac Thermo Lid to the required temperature before starting the application.

Heating the Vac Thermo Lid to 110 °C takes approx. 30 min.

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5.6.5 Elution plate adapter

Abb. 5-15: Elution plate adapter

Fig. 5-15: Elution plate adapter

If you are using tube plates as collection vessels you need to use the elution plate adapter. The elution plate adapter ensures that the tube plates sit firmly in one position.

The eluation adapter is made from stainless steel.

5.6.5.1 Using the elution plate adapter

1. Place the elution plate adapter on the edge of the tube plate.

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5.6.6 Loading the vacuum unit

1VAC Lid

2Filter plate

4 Collection vessel

5 Vacuum unit

3 Vacuum frame

Load the vacuum unit as follows:

1. Place the collection vessel on the vacuum unit.

2. If applicable, place channeling plate 96 on the collection vessel.

3. Place the vacuum frame on the collection vessel.

The weight of the vacuum frame presses the collection vessel into the vacuum unit.

If the vacuum frame is incorrectly positioned, the vacuum will not be generated.

4. Place the filter plate in the vacuum frame.

5. If applicable, place the VAC Lid or the VAC Thermo Lid on the filter plate.

The label on the VAC Lid and the Vac Thermo Lid must face the front

6. The VAC Lid and Vac Thermo Lid can be transported during the application. For the transport, place the VAC Lid on the gripper holder and the VAC Thermo Lid on a thermal module.

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5.7 CleanCap

The CleanCap is optional equipment for the epMotion and can only be ordered with a new device. The CleanCap includes a HEPA filter and a UV lamp.

5.7.1 HEPA filter

WARNING! Risk of infection due to aerosols.

If you work with the HEPA filter, a continuous air flow will be blown from the epMotion workspace to the ambience. The air flow can transport aerosols and contaminate the ambience.

 Do not use the HEPA filter when working with biohazardous substances.  Disinfect contaminated surfaces in the epMotion workspace before switching on the HEPA

filter.

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The HEPA filter will become dirty over time. Have the HEPA filter replaced by Eppendorf Service once per year.

The HEPA filter cleans the air in the epMotion workspace. The HEPA filter reduces the amount of particles such as dust or germs.

The fan continuously draws air out of the ambience and channels it to the HEPA filter. The filtered air flow will be routed to the epMotion workspace.

5.7.1.1 Using HEPA filters

Use the HEPA filter as follows:

Prerequisites

• The epMotion is ready for operation.

1. Decontaminate the surfaces in the epMotion workspace.

2. Decontaminate labware and dispensing tools.

3. Close the front hood.

4. Switch on the HEPA filter using the software. (refer to the epMotion software operating manual).

5. Let the HEPA filter run for 5 min.

All of the air in the epMotion workspace has been filtered.

6. Equip the epMotion.

7. Close the front hood.

8. Start the application.

The HEPA filter remains switched on after the end of the application.

9. Start another application, or switch off the HEPA filter using the operating software.

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5.7.2 UV lamp

CAUTION! Risk to health due to UV radiation.

UV radiation causes eye and skin damage. UV radiation may escape outward through the ventilation slits in the rear panel of the epMotion.

 Wear your personal protective equipment when you are near the ventilation slits.

NOTICE! Damage to plastic due to UV radiation.

Plastics become brittle due to exposure to UV radiation.

 Remove the dispensing tools, labware and waste container from the worktable before

switching on the UV lamp.

The radiated power of the UV lamp decreases with age. Have the UV lamp replaced by Eppendorf Service once every 2 years.

The UV lamp decontaminates the worktable. An irradiation cycle lasts 15 min. The UV lamp is controlled by the software.

5.7.2.1 Using UV lamps

Prerequisites

• The epMotion is ready for operation.

1. Remove the labware, waste container and dispensing tools from the worktable.

2. Close the front hood.

3. Switch on the UV lamp using the software (refer to the epMotion software operating manual).

When you open the front hood, the UV-lamp switches off.

Information on decontaminating labware and dispensing tools is available (see

Decontamination and cleaning on p. 97)

5.8 Barcode reader

Abb. 5-16: Model of a barcode reader

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Fig. 5-16: Model of a barcode reader

1Window 2Key

With the barcode reader you can track and document your samples via barcode IDs.

The barcode reader is connected to a USB port on the MultiCon.

Plates have a barcode ID. The barcode ID of a well is generated automatically from the barcode ID of the plate and the identification of the well. Racks do not have a barcode ID. Each tube in a rack has a barcode ID.

The barcode reader has 2 settings:

• Manual: To read a barcode ID, press a key on the barcode reader.

• Automatic: To read a barcode ID, hold the barcode reader to the barcode. The barcode reader detects the barcode IDs automatically.

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5.9 Tools

NOTICE! Damage to the gold contacts from handling.

The tool is damaged if the gold contacts on the tool become damaged or contaminated.

 Do not touch the gold contacts.

The tool holder picks up a dispensing tool and puts it down again. The tool holder recognizes the tool from its gold contacts.

The tool holder also picks up the gripper.

5.9.1 Dispensing Tools

Abb. 5-17: Single-channel dispensing tool and eight-channel dispensing tool

Fig. 5-17: Single-channel dispensing tool and eight-channel dispensing tool

The dispensing tools are piston-stroke pipettes. The function of the dispensing tools is described in the chapter (see Dispensing liquids on p. 53).

Single-channel dispensing tools and eight-channel dispensing tools, with 3 volume ranges each, are available for the epMotion.

Tab. 5-3: Volume range of the dispensing tools

Single-channel dispensing tool Multi-channel dispensing tool Volume range

TS 50 TM 50 1 µL – 50 µL

TS 300 TM 300 20 µL – 300 µL

TS 10000 TM 1000 40 µL – 1000 µL

The dispensing tool errors can be found in the technical data (see p. 115).

Help on dispensing errors can be found in the "Maintenance" chapter (see Dispensing errors on p. 107)

5.9.2 Gripper

CAUTION! Risk of injury from mandrels on the gripper arms.

Each gripper arm has 2 sharp mandrels on the inside.

 Handle the gripper from above.  Do not touch the gripper arms on the inside.

Abb. 5-18: Gripper with gripper holder

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Fig. 5-18: Gripper with gripper holder

The gripper transports labware. The gripper uses the mandrel and peg to hold the labware on the gripper arms. The gripper is placed on the gripper holder.

The gripper transports the following labware: The gripper does not transport the following

labware:

•Plate All plates will be transported. Plates will be placed on a worktable, stack, height adapter or thermoadapter.

•Thermoblock Will be transported with the positioned plate. The positioned plate will not be picked up by the gripper without the thermoblock.

•Thermorack Will be transported with the inserted reaction vessels. The inserted reaction vessels will not be picked up by the gripper without the thermorack.

• Individual vessels

•Plate stack The top plate will be transported from a plate stack.

• epT.I.P.S. Motion Racks

• Racks

• Reservoir Racks

• Reservoirs and Reservoir Rack modules

• Height adapter

• Thermoadapter A plate will be transported if it is inserted in a thermoadapter.

•ReagentRack

•PrepRack Will be transported with the inserted reaction vessels. The inserted reaction vessels will not be picked up by the gripper without the thermorack.

• 300 mL reservoir

• 400 mL reservoir

• Vacuum frame Is transported on its own or with the filter plate attached.

• VAC Lid and Vac Thermo Lid

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6 Dispensing liquids

upper end position

basic position

end of blow-out

AB C

6.1 Basic procedure for dispensing

6.1.1 Operating principle of dispensing tools

Abb. 6-1: Piston-stroke pipette principle

Fig. 6-1: Piston-stroke pipette principle

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AAspirate

The piston moves upward from the home position and aspirates liquid.

BDispense

Liquid dispensing.

CBlow-out

Dispenses the residual liquid Blow-out in the Pipette dispensing mode Remaining stroke in the Multidispense dispensing mode

Multidispense: Piston travels to the home position

in separate steps.

Pipette: Piston travels to the home position in one

step.

The dispensing tools are piston-stroke pipettes that function according to the air cushion principle.

If the piston moves upward in the dispensing tool, liquid will be aspirated into the pipette tip. If the piston moves downward in the dispensing tool, liquid will be dispensed from the pipette tip. The pistons will be moved by a motor in the tool holder.

On eight-channel dispensing tools, all 8 channels move simultaneously.

1 mm

0,7 mm

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6.1.2 Liquid aspiration up to the remaining volume

Abb. 6-2: Liquid aspiration up to the remaining volume with a constant immersion depth

Fig. 6-2: Liquid aspiration up to the remaining volume with a constant immersion depth

The pipette tip is immersed 3 mm into the liquid before liquid aspiration. The pipette tip moves downward during liquid aspiration. The immersion depth of 3 mm remains constant while the liquid level decreases.

If the pipette tip reaches a distance of 1 mm to the base of the vessel, the pipette tip reduces its immersion depth to 0.7 mm. If the liquid level continues to sink, the pipette tip will not aspirate any additional liquid. The remaining volume remains in the vessel.

A remaining volume is present because the pipette tip has a minimum immersion depth in the liquid and a minimum distance to the base of the vessel. Under normal conditions, the remaining volume is calculated using a filling level of 1.7 mm.

The distance of the pipette tip to the base of the vessel can be changed in the software (refer to the epMotion software operating manual).

If the liquid is aspirated from the remaining volume, the bend of the liquid level can lead to dispensing errors.

6.1.3 Bottom tolerance

The bottom tolerance is the distance between the vessel base and the lower end of the pipette tip. The default setting for the bottom tolerance is 1 mm. For 30 mL and 100 mL reservoirs, the default setting for the bottom tolerance is 2.5 mm.

Decreasing the bottom tolerance also reduces the remaining volume.

1. Removing liquid above a pellet increases the bottom tolerance.

2. Do not decrease the bottom tolerance on uneven plates.

3. Check the set bottom tolerance when exchanging pipette tips, plates or vessels.

6.1.4 Transporting and dispensing liquid

~ 3 - 4 mm

~ 6 - 8 mm

Abb. 6-3: Transporting and dispensing liquid

Fig. 6-3: Transporting and dispensing liquid

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A Dispensing liquid B Retraction of liquid after liquid aspiration

6.1.4.1 Dispensing liquid

The liquid from the pipette tip will be dispensed in the free jet. The liquid will be dispensed into the vessel 3 mm – 4 mm above the surface of the liquid. The pipette tip moves upward during dispensing. The distance from the pipette tip to the surface of the liquid remains constant.

For liquid type ProteinC, a distance of 5 mm from the pipette tip to the surface of the liquid is set in the software. This setting takes into account the tendency of concentrated protein solutions to form foam.

6.1.4.2 Transporting liquids

The liquid is drawn into the pipette tip before transport. An area filled with air is created in the lower part of the pipette tip. The liquid does not drip during transport.

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6.2 Dispensing modes

The epMotion can be used to dispense liquids in the Pipette and Multidispense modes.

6.2.1 Dispensing mode: Pipette

In the Pipette mode, a defined volume of liquid is aspirated and fully dispensed.

Dispensing errors are smaller than in the Multidispense (see Dispensing tool errors on p. 115) mode.

The epMotion executes a blow-out in the Pipette mode. Residual liquid from the pipette tip will be dispensed in the destination vessel. The blow-out is part of the dispensing volume. The blow-out can be set in the software (refer to the epMotion software operating manual).

In the Pipette mode, the smallest dispensing volume for water is 1 L.

6.2.2 Dispensing mode: Multidispense

In the Multidispense mode, liquid is aspirated and dispensed in the destination vessels in defined partial quantities.

Dispensing errors are larger than in the Pipette (see Dispensing tool errors on p. 115) mode.

The epMotion carries out a remaining stroke in the Multidispense mode. Residual liquid from the pipette tip will be dispensed in the waste vessel or source vessel. The residual liquid is not part of the dispensing volume.

In the Multidispense mode, the smallest dispensing volume for water is 3 L.

6.2.2.1 Aspiration volume at Multidispense

To create the same physical conditions for all dispensing steps, more liquid must be aspirated in the

Multidispense mode than the sum of the dispensing volumes. This is the result of the reverse stroke and the

remaining stroke.

Reverse stroke

• The reverse stroke occurs after liquid aspiration. Part of the aspirated volume is immediately returned to the source vessel. The reverse stroke is the same size for all liquids.

Remaining stroke

• After the reverse stroke, there is more liquid in the pipette tip than the amount required for the dispensing steps. This additional aspiration volume will be dispensed as a remaining stroke after dispensing. The additional aspiration volume will be dispensed to the source vessel if the tips are not replaced before each liquid aspiration. The additional aspiration volume will be dispensed to the waste container if the tips are not replaced before each liquid aspiration.

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The volume for the additional aspriation depends on the dispensing tool; the software will take it into account when calculating the required aspiration volume.

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Dispensing tool Additional volume per

channel for reverse stroke

Single-channel dispensing tool TS 50 Eight-channel dispensing tool TM 50

Single-channel dispensing tool TS 300 16.7 µL 3.7 µL

Eight-channel dispensing tool TM 300 45.2 µL 5.0 µL

Single-channel dispensing tool TS 1000 Eight-channel dispensing tool TM 1000

Example for aspiration volume for multi-dispensing

A 96-well plate should be filled with 10 L water in each well via multi-dispensing. The eight-channel dispensing tool TM 50 is used. Aspiration is performed from one reservoir. The tips are not changed before a new liquid aspiration.

Sum of the aspiration volumes for multi-dispensing:

• 96 x 10 L for 96-well plate: 960 L

• 8 x 5.8 L reverse stroke: 46.4 L

• 8 x 2.5 L additional aspiration: 20 L

•Sum: 1026.4 L

5.8 µL 2.5 µL

50.3 µL 35.2 µL

Additional volume per channel for remaining stroke

6.2.3 Mixing

Liquids can be mixed via multiple pipetting.

A mixing cycle consists of one upward movement and one downward movement of the piston in the dispensing tool.

The aspiration level and dispensing level of the liquid can be defined during mixing. A defined height should only be used for levels below the working volume as the vessel may overflow with larger levels.

During the mixing process, the pipette tip is in the liquid. The blow-out will be executed above the surface of the liquid after the mixing procedure.

Defined mixing speeds are defined in the liquid types. When using your own settings, determine the optimal mixing speed via experiments.

NOTICE! Contamination of the dispensing tool due to high mixing frequency.

If the mixing frequency is too high, liquid may enter the dispensing tool.

 If the liquid has a low viscosity or tends to foam, set a low mixing frequency.  Use demineralized water to test the mixing settings.  Use pipette tips with filter.

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6.2.3.1 Recommended mixing speeds

You can set mixing speeds of 0.2 mm/s – 110 mm/s.

Dispensing tool Mixing speed of lower

volume range in mm/s

Mixing speed of medium volume range

Mixing speed of upper volume range in mm/s

in mm/s

TS 50 15 – 88 15 – 44 10 – 40

TM 50 15 – 88 15 – 44 10 – 40

TS 300 5 – 15 6 – 16 6 – 16

TM 300 2 – 11 2 – 11 2 – 11

TS 1000 4 – 15 4 – 15 4 – 15

TM 1000 4 – 15 4 – 15 4 – 15

6.3 Special features

6.3.1 Liquid aspiration from bottom of vessel

The pipette tip can also be positioned above the bottom of the vessel at the height of the bottom tolerance. This prevents the pipette tip from changing its position during liquid aspiration.

The level in the vessels may not exceed the working volume. If the level exceeds the working volume, liquid may overflow.

Liquid aspiration from the bottom of the vessel is recommended in the following cases:

• For vessels ≤0.5 mL, 96-well plates and 384-well plates

• For plates with various filling volumes in the wells

•For vessels with low filling volumes

Liquid aspiration from the bottom of the vessel can be activated in the software using the Aspirate from

bottom option (refer to the epMotion software operating manual). The bottom tolerance, which can be set in

the software, depends on the labware.

Abb. 6-4: Liquid displacement in vessels

Fig. 6-4: Liquid displacement in vessels

A Vessel filled up to the working volume B Vessel with maximum immersed tip before

liquid aspiration

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6.3.2 Liquid aspiration from high vessels

Observe the following special features when using high vessels, e.g., 15 mL centrifuge tubes.

6.3.2.1 Liquid aspiration from bottom of vessel

Abb. 6-5: Large vessel with high liquid level

Fig. 6-5: Large vessel with high liquid level

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If you use vessels > 3 mL with high levels, do not aspirate liquids from the bottom of the vessel. This will result in the outside of the pipette tip being wetted, which increases the risk of contamination and distorts the dispensing result. For very large vessels with high levels, the entire pipette tip and the tip cone of the dispensing tool will be wetted.

6.3.2.2 High remaining volume

Abb. 6-6: Liquid aspiration from high vessels with 1000 µL, 50 µL and 300 µL pipette t ips

Fig. 6-6: Liquid aspiration from high vessels with 1000 µL, 50 µL and 300 µL pipette tips

50 L tips and 300 L tips do not extend as far into the vessels as 1000 L tips. In high vessels, 50 L and 300 L tips do not reach the bottom of the vessels. If you use 50 L and 300 L tips, the remaining volume will be larger than with 1000 L tips.

Work with the 1000 µL dispensing tool when you use high vessels. If you use the 50 µL dispensing tool or the 300 µL dispensing tool, and would like to reduce the remaining volume, select the lower vessels.

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6.3.3 Liquid dispensing at the height of the vessel edge

In the software, the pipette tip can be positioned at a height of 3 mm – 4 mm below the edge of the vessel using the Dispense from top option. The height of the pipette tip does not change.

Use the option for:

•Quick dispensing

• Dispensing in vessels with small diameters, e.g., 384-well plates, capillaries The tips remains in the upper area of the vessel and do not travel further into the vessel. This virtually eliminates the possibility of the pipette tip becoming contaminated.

• Dispensing in plates with vastly different levels in the wells

6.3.4 Liquid aspiration from a filter plate

In the software, liquid can be suctioned from the supernatant of filter plates and filter vessels via the Elution

from filter option.

The Elution from filter option refers to the source vessel and almost fully aspirates liquid from the filter plate. The pipette tip touches the filter. The piston movement in the dispensing tool for liquid aspiration starts when the pipette tip moves in the vessel. Maximum stroke is used on each dispensing tool. This also applies to dispensing.

When the liquid is being transported, air bubbles may be present at several locations in the pipette tip. The size of the air bubble on the lower end of the pipette tip may vary.

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7 Labware – vessels, plates and pipette tips

The term labware refers to epMotion consumables and accessories. Consumables are vessels, plates and pipette tips. Accessories are adapters, blocks and racks. epBlue includes a definition for labware that is placed on the epMotion worktable. The definition includes data on the geometry, tempering ability, volume and bottom tolerance of the labware.

The epMotion can be used with a variety of labware from various manufacturers. Information on how to expand the labware library can be found in the software operating manual.

Labware may not exceed an overall height of 126 mm. The software generates an error

Abb. 7-1: Reaction vessel with lid

message if this overall height is exceeded.

1.5

1.0

0.5

0.1

3 4

Fig. 7-1: Reaction vessel with lid

1 Filling volume

2 Working volume

3 Detection limit of optical sensor

4 Remaining volume

7.1 Tubes

The vessels will be inserted in racks. All reaction vessels which fit in an epMotion rack can be used.

Examples of reaction vessels that can be used:

•Safe-Lock tubes

• Standard reaction vessels 3810X

• PCR vessels

• Conical tubes/centrifuge tubes

•Cryotubes

Individual reaction vessels are not transported with the gripper.

Level measurement using the optical sensor is more difficult with 0.2 mL and 0.5 mL tubes.

Use Thermoracks or Reservoir Rack modules to temper vessels.

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7.2 Plates

Abb. 7-2: Plates: left 96-well MTP, middle 24-well MTP, right 96-well DWP

Fig. 7-2: Plates: left 96-well MTP, middle 24-well MTP, right 96-well DWP

The following plates are available for the epMotion:

• DWP plates with 24, 96 or 384 wells

• MTP plates with 6, 24, 48, 96 or 384 wells

• Filter plates for use with the vacuum unit

• PCR plates with 96 or 384 wells

• Vessel plates with 96 individual vessels (tube plates)

Skirted plates can be placed directly at a worktable location. Plates can be placed on adapters.

Plates are transported with the gripper. Stacked plates will not be transported together.

Stacking plates

If they are manufactured by Eppendorf, the same type of plates can be stacked on the worktable. If you would like to stack plates, use Eppendorf plates. Plates from other manufacturers may have deviating dimensions, which will prevent them from being stacked.

7.2.1 Deepwell plates

Abb. 7-3: Deepwell plate with 96 wells

Fig. 7-3: Deepwell plate with 96 wells

A maximum of 2 Eppendorf DWP plates can be stacked at one location.

A thermoadapter is available for the Eppendorf 1.0 mL deepwell plate.

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7.2.2 Microplates

Abb. 7-4: Microplate wi th 96 wells

Fig. 7-4: Microplate with 96 wells

A maximum of 5 Eppendorf MTP plates can be stacked at one location.

A level measurement with optical sensor cannot be completed for an MTP with 384 wells. A level measurement with optical sensor is not recommended for an MTP with 96 wells.

7.2.3 Filter plates

Abb. 7-5: Filter plate with 96 wells

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Fig. 7-5: Filter plate with 96 wells

Filter plates are available for nucleic acid purification with a vacuum unit.

Each well of a filter plate includes a filter and an opening at the bottom.

7.2.4 Channeling plate 96

Abb. 7-6: Channeling plate 96

Fig. 7-6: Channeling plate 96

The channeling plate 96 is used in the vacuum unit. The Channeling plate 96 protects the filter plate against contamination.

The channeling plate 96 is positioned on the collection vessel in the vacuum unit. If you insert the Channeling Platte 96 you can only use the 400 mL reservoir as the collection vessel.

Due to the geometry of the filter plates, the Channeling Plate 96 is recommended for Qiagen applications (QIAamp 96 DNA Blood and QIA_Rneasy 96).

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7.2.5 PCR plates

Abb. 7-7: PCR plate with 96 wells

Fig. 7-7: PCR plate with 96 wells

PCR plates are available with 96 and 384 wells.

96-well PCR plates are available with a border (skirted), a semi-border (semi-skirted) or without a border (unskirted).

96-well PCR plates with a semi-border or without a border can only be positioned and transported with the Thermoblock PCR 96.

Tab. 7-1: Placing PCR plates on the worktable

Plate With thermoblock With thermoadapter Direct

96-well PCR plates skirted x x x

96-well PCR plates semi-skirted x

96-well PCR plates unskirted x

7.2.6 Tube plates

Tube plates are plates with individual vessels. Tube plates are available in various vessel layouts. Tube plates are treated as normal plates.

7.3 Reservoirs

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Abb. 7-8: 100 mL and 30 mL reservoirs

Fig. 7-8: 100 mL and 30 mL reservoirs

Abb. 7-9: Reservoir 400 mL

Fig. 7-9: Reservoir 400 mL

Reservoirs make liquid available. Reservoirs are available in 30 mL, 100 mL and 400 mL sizes. Reservoirs for 30 mL and 100 mL are placed in a Reservoir Rack.

Reservoirs are made of polypropylene (PP).

Properties of 30 mL and 100 mL reservoirs

• 30 mL reservoirs can be used with all tips.

• The 30 mL reservoirs are very tight at the bottom. If the bottom tolerance is reduced, reservoirs for 30 mL can also be lifted using pipette tips.

• Reservoirs for 30 mL and 100 mL have a ribbed base. If the bottom tolerance has been reduced, the information on the remaining volume is not precise.

• Reservoirs for 30 mL and 100 mL are particularly well-suited for eight-channel dispensing tools.

• The eight-channel dispensing tools TM 50 and TM 300 cannot be immersed all the way to the bottom in the 100 mL reservoir. A higher remaining volume remains in the reservoir.

Properties of 400 mL reservoirs

• The remaining volume of the 400 mL reservoir is approx. 10 mL.

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7.4 epT.I.P.S. Motion

NOTICE! Dispensing error due to incorrect handling of pipette tips.

Tips become deformed and change size during autoclaving.

 Do not autoclave the pipette tips. Use tips with the sterile specification, if required.  Do not stack any racks that contain pipette tips.

Observe the instructions for use for the epT.I.P.S. Motion Racks and epT.I.P.S. Motion Reloads.

Abb. 7-10: epT.I.P.S. Motion 1 000 µL, 50µL, 300 µL

Fig. 7-10: epT.I.P.S. Motion 1 000 µL, 50µL, 300 µL

epT.I.P.S. Motion are special pipette tips for the epMotion.

epT.I.P.S. Motion pipette tips, racks and trays are made from polypropylene (PP). The filter is made of polyethylene (PE).

Racks and trays are not transported with the gripper.

The optical sensor detects the size of the pipette tips via the rack coding. The optical sensor also detects if the pipette tips have a filter. However, the optical sensor does not use the coding to detect if the pipette tips are in racks, SafeRacks or in the Tip Holder.

Labware – vessels, plates and pipette tips

7.4.1 epT.I.P.S. Motion Racks

Abb. 7-11: epT.I.P.S. Motion Ra ck

Fig. 7-11: epT.I.P.S. Motion Rack

epT.I.P.S. Motion racks are intended for single use.

epT.I.P.S. Motion racks are specially designed for applications that require sterile work.

epMotion

English (EN)

5075

Tab. 7-2: epT.I.P.S. Motion racks are available in the following sizes:

Size of the pipette tips Dispensing tool Color of the racks

50 µL TS 50, TM 50 Light gray

300 µL TS 300, TM 300 Yellow

1000 µL TS 1000, TM 1000 Dark blue

Tab. 7-3: epT.I.P.S. Motion racks are available in the following purity grades:

epT.I.P.S. Motion Racks Purity grade

Pipette tips with filter PCR clean

PCR clean and Sterile

Pipette tips without filter Eppendorf Quality

Sterile

Labware – vessels, plates and pipette tips

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7.4.2 epT.I.P.S. Motion Reloads

Abb. 7-12: epT.I.P.S. Motion Reloads in individual packaging

Fig. 7-12: epT.I.P.S. Motion Reloads in individual

Abb. 7-13: epT.I.P.S. Motion Reloads an d tip holder

Fig. 7-13: epT.I.P.S. Motion Reloads and tip holder

packaging

epT.I.P.S. Motion Reloads are intended for single use.

The pipette tips are in trays. The trays will be placed in reusable tip holders, which reduces waste because no racks are required.

Tab. 7-4: epT.I.P.S. Motion Reloads are available in the following sizes:

Size of the pipette tips Dispensing tool Tray color

50 µL TS 50, TM 50 Light gray

300 µL TS 300, TM 300 Yellow

1000 µL TS 1000, TM 1000 Dark blue

Tab. 7-5: epT.I.P.S. Motion Reloads are available in the following purity grades:

epT.I.P.S. Motion Reloads Purity grade

Pipette tips with filter PCR clean

Pipette tips without filter Eppendorf Quality

Labware – vessels, plates and pipette tips

7.4.3 Tip holder for epT.I.P.S. Motion Reloads

Abb. 7-14: Tip Holder

Fig. 7-14: Tip Holder

The tip holder is an adapter that holds the epT.I.P.S. Motion Reloads.

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7.4.3.1 epT.I.P.S. Motion Reloads on the tip holder

Abb. 7-15: Placing the tray on the tip holder

Fig. 7-15: Placing the tray on the tip holder

1Tips

2Tray

1. Open the Reload packaging.

2. Remove the tray with the tips.

3. Place the tray on the tip holder. The notch in the tray must be on the labeled side of the tip holder.

3Tip Holder

Labware – vessels, plates and pipette tips

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7.4.4 epT.I.P.S. Motion SafeRacks

Abb. 7-16: epT.I.P.S. Motion SafeRack

Fig. 7-16: epT.I.P.S. Motion SafeRack

NOTICE! Contamination due to using incorrect pipette tips.

The optical sensor does not detect if the pipette tips on the worktable are intended for single use (epT.I.P.S. Motion Rack, epT.I.P.S. Motion Reload) or multiple use (epT.I.P.S. Motion SafeRack).

 If the pipette tips will be used several times, equip the entire worktable with epT.I.P.S.

Motion SafeRacks.

The epT.I.P.S. Motion in SafeRacks are intended for multiple use. If you use the pipette tips in an application, use epT.I.P.S. Motion SafeRacks, for example, to remove buffers in several washing steps.

epT.I.P.S. Motion SafeRacks have a partition to separate pipette tips. During an application, used pipette tip are returned to the SafeRack. A partition prevents residual liquid from contaminating adjacent pipette tips. Contamination of samples due to repeated use is prevented because the software permanently allocates the pipette tips within a specific application to a source vessel.

In the software, you can set whether pipette tips should be used multiple times (refer to the epMotion software operating manual).

Use epT.I.P.S. Motion SafeRacks for a maximum of 6 usage cycles. A usage cycle consists of aspiration, dispensing and ejecting the tip.

Tab. 7-6: epT.I.P.S. Motion SafeRacks are available in the following sizes:

Size of the pipette tips Dispensing tool Rack color

50 µL TS 50, TM 50 Light gray

1000 µL TS 1000, TM 1000 Dark blue

Tab. 7-7: epT.I.P.S. Motion SafeRacks are available in the following purity grades:

epT.I.P.S. Motion SafeRacks Purity grade

Pipette tips with filter PCR clean

Pipette tips without filter Eppendorf Quality

8 Labware accessories

Vessels and accessories may not exceed an overall height of 126 mm. If this overall height is exceeded, the software generates an error message.

8.1 Racks

Racks are holders for mounting the same type of individual vessels.

Racks are not transported with the gripper.

8.1.1 Racks for 24 vessels

Abb. 8-1: Rack for 24 vessels

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Fig. 8-1: Rack for 24 vessels

Various racks are available for vessels 12 mm – 17 mm in diameter. Racks are available in 2 heights. The bores for the vessels are numbered.

The racks cover the same area as plates. Racks can be positioned at any location.

Racks are coded. The optical sensor uses the code to detect if the rack is correctly positioned.

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8.1.2 Rack for 96 vessels

Abb. 8-2: Rack 96

Fig. 8-2: Rack 96

The Rack 96 takes up 2 locations on the worktable. The rack holds 96 tubes without lids, with a volume of

1.5 mL or 2 mL.

8.1.2.1 Equipping Rack 96

The Rack 96 can be equipped with vessels with fastened lids, e.g., Safe-Lock tubes.

 If you equip the Rack 96 with Safe-Lock tubes, leave the second row of the rack open.

 Ensure that the lids do not cover the openings of adjacent vessels.

8.1.2.2 Placing the Rack 96 on the worktable

1. Place the Rack 96 on the epMotion workable so the opening in the lower panel points toward the user.

2. Place the Rack 96 on the positioning pins of the two locations.

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8.1.3 Rack LC

Abb. 8-3: Rack LC 100 µL

Fig. 8-3: Rack LC 100 µL

The Rack LC can hold 96 LightCycler capillaries with a 20 L filling volume or 96 capillaries with a 100 L filling volume. The bores for both sizes of capillary are arranged in an alternating pattern.

The Rack LC is labeled on both sides. The label on one side indicates the capillary size of 20 µL. The label on the other side indicates the capillary size of 100 µL.

8.1.3.1 Equipping the Rack LC and positioning it on the worktable

 Equip the Rack LC. Only use capillaries of the same size for a single application.

 Place the Rack LC on the epMotion worktable.

The label that lists the size of the inserted capillary must point toward the user.

8.1.4 Thermorack and Rack 0.5/1.5/2.0 mL

Abb. 8-4: Thermorack 0.5 mL; 1.5 mL; 2 mL

Fig. 8-4: Thermorack 0.5 mL; 1.5 mL; 2 mL

Thermoracks and the 0.5/1.5/2.0 mL racks have 24 bores for tubes with a volume of 1.5 mL and 2 mL. Vessels with a volume of 0.5 mL can be inserted with adapter sleeves.

Abb. 8-5: Rack 0.5 mL; 1.5 mL; 2 mL

Fig. 8-5: Rack 0.5 mL; 1.5 mL; 2 mL

Thermoracks and 0.5/1.5/2.0 mL racks have lid holders. The lid holders are on the right, next to the bore for the vessel. The lid holder vertically clamps the tube lids.

Thermoracks and the 0.5/1.5/2.0 mL racks are transported using the gripper. Thermoracks are suitable for temperature control and designed for use on thermal modules.

Thermoracks can be cooled in the lab refrigerator.

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Thermorack TMX

Thermoracks TMX are designed for use with the thermomixer. The Thermorack TMX is lighter than a standard thermorack and enables higher speeds during mixing. The Thermorack TMX has a low thermal capacity, but a fast heat transfer. The Thermorack TMX quickly reaches the target temperature, but cannot maintain this temperature for a long time after temperature control.

The Thermorack TMX is available with installed adapters for vessels with a volume of 0.5 mL.

Tab. 8-1: Test values for temperature control of thermoracks and thermoadapters

Thermorack/thermoadapter Equipping Filling volume 

per vessel

Heating time  from 0 °C – 10 °C

Thermorack 1.5/2.0 mL 1.5 mL Safe-Lock tube 1000 µL ~ 30 min

Thermoadapter PCR 96 96-well twin.tec PCR plate 150 µL ~ 14 min

Thermoadapter PCR 384 384-well twin.tec PCR plate 25 µL ~ 10 min

8.1.5 PrepRack

Abb. 8-6: PrepRack

Fig. 8-6: PrepRack

The PrepRack is specially designed for use with the magnet finger module. You can also use the PrepRack as the standard rack.

The PrepRack has 24 vessel bores which are numbered by row-by-row. The corresponding lid holder is at the right of the vessel bore. The lid holder vertically clamps the tube lids.

You can equip the PrepRack in the same way as the thermorack and the 0.5/1.5/2.0 mL rack (see p. 73).

The PrepRack is designed to be used on the thermomixer and can be tempered.

The optical sensor detects the PrepRack using its code.

The PrepRack is transported with the gripper.

8.1.6 ReagentRack

Abb. 8-7: ReagentRack

Fig. 8-7: ReagentRack

The ReagentRack holds a tray with reagents from the Eppendorf MagSep kits.

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The ReagentRack cannot be placed on the TMX.

The optical sensor detects the tray with reagents using its code.

The ReagentRack is not transported with the gripper.

8.1.6.1 Positioning the ReagentRack on the worktable

 The ReagentRack cannot be placed at location B0.

 Place the ReagentRack on the worktable so that the Reservoir Rack tabs are positioned between the

locating pins for the location.

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8.1.7 Reservoir Rack

Abb. 8-8: Equipped Reservoir Rack

Fig. 8-8: Equipped Reservoir Rack

The Reservoir Rack (4) is used to hold reservoirs (1) and Reservoir Rack modules (2). The Reservoir Rack columns are numbered (3).

The Reservoir Rack can hold a maximum of 7 reservoirs (1) or 7 different Reservoir Rack modules (2). Reservoir Racks can be loaded in any order.

The ReservoirRack cannot be placed on the thermomixer.

8.1.7.1 Loading Reservoir Racks

The temperature control of the Reservoir Rack modules in the outer positions is not as good.

 Insert Reservoir Rack modules and reservoirs in the Reservoir Rack with the code facing the rear.

8.1.7.2 Positioning the Reservoir Rack on the worktable

 The Reservoir Rack cannot be placed at A locations or location B0.

 Place the Reservoir Rack on the worktable so the Reservoir Rack tabs are positioned between the

positioning pins for the location.

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8.1.8 ReservoirRack 3

Abb. 8-9: ReservoirRack 3

Fig. 8-9: ReservoirRack 3

The ReservoirRack 3 can be used as a waste container for liquid waste. The ReservoirRack 3 can be equipped with up to 3 reservoirs for 30 mL or 100 mL. The ReservoirRack 3 cannot be equipped with Reservoir Rack modules.

8.1.8.1 Positioning the Reservoir Rack 3 on the worktable

You can only position the ReservoirRack 3 in location A5 of the epMotion 5075m and 5075t.

 Place the ReservoirRack 3 on the worktable in such a way that the Reservoir Rack tabs are positioned

between the positioning pins for the location.

8.1.9 Reservoir Rack modules

Abb. 8-10: Versions of Reservoir Rack modules

Fig. 8-10: Versions of Reservoir Rack modules

1 Reservoir Rack module TC Ø 29 mm

2 Reservoir Rack module TC Ø 17 mm

3 Reservoir Rack module TC reservoir 100 mL

4 Reservoir Rack module TC reservoir 30 mL

5 Reservoir Rack module TC Ø 16 mm

6 Reservoir Rack module TC Ø 12 mm

7 Reservoir Rack module TC Safe-Lock

8 Reservoir Rack module TC PCR 0.5 mL

9 Reservoir Rack module TC PCR 0.2 mL

10 Reservoir Rack module TC Eppendorf tubes 5 mL

Reservoir Rack modules are equipped with vessels and inserted into a Reservoir Rack. All of the vessels equipped in a Reservoir Rack module must be of the same type.

The Reservoir Rack modules TC are suitable for temperature control.

Labware accessories

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8.1.9.1 Connecting 30 mL/100 mL TC Reservoir Rack modules to an adjacent module

Abb. 8-11: Connecting the 30 mL/100 mL TC Reservoir Rack module to an adjacent mod ule

Fig. 8-11: Connecting the 30 mL/100 mL TC Reservoir Rack module to an adjacent module

1 Reservoir

2 30 mL/100 mL TC Reservoir Rack modules

3 Connecting web

4 Adjacent module

The 30 mL/100 mL TC Reservoir Rack modules must be mounted to the next Reservoir Rack module using 2 connecting bars.

 Insert the 2 connecting bars for the TC Reservoir Rack modules in the next Reservoir Rack modules.

8.1.9.2 Setting the Reservoir Rack module to vessel height

Abb. 8-12: Setting the Reservoir Rack module to vessel height

Fig. 8-12: Setting the Reservoir Rack module to vessel height

1 Positioning screw

3 Reservoir Rack module

In storage position

2 Positioning screw

At 50 mm height setting

The Reservoir Rack modules have bores 12 mm, 16 mm and 17 mm in diameter at the front and rear. The bores are mounted at 50 mm, 60 mm, 70 mm, 80 mm and 90 mm. Use the 2 positioning screws to position the vessels at these heights.

 Screw the 2 positioning screws into the bores at the required height.

Tab. 8-2: Temperature control duration of Reservoir Rack modules and reaction vessels

Temperature change

from 23°C to 4°C

Reservoir Rack module TC Tube Set

temperature

Temperature control time

Tem pera ture chang e from 23°C to 37°C

Set temperature

RR module TC PCR 0.2 mL PCR reaction vessel

0.2 mL

3°C

Approx.  15 min

38 °C

RR module TC PCR 0.5 mL PCR reaction vessel

0.5 mL

Safe-Lock tube

0.5 mL 3°C

RR module TC Safe-Lock

Safe-Lock tube

1.5 mL

Safe-Lock tube

2 °C

3°C

Approx.  20 min

38 °C

2.0 mL

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Temperature control time

Approx.  8 min

Approx.  12 min

RR module TC Ø 12 mm Reaction vessel 

3°C

Ø 12 mm

RR module TC Ø 16 mm Reaction vessel 

Ø 16 mm

RR module TC 

Tube 5 mL 3°C

3°C

Approx.  30 min

38 °C

Approx.  17 min

Eppendorf tubes 5 mL

RR module TC Ø 17 mm Conical tube 15 mL 2 °C

RR module TC Ø 29 mm Conical tube 50 mL

RR Module TC 

Reservoir 30 mL

3°C

1°C

Approx.  39 min

Approx.  21 min

39 °C

Approx.  23 min

Approx.  15 min

Reservoir 30 mL

RR Module TC 

Reservoir 100 mL

1°C

Approx.  46 min

40 °C

Approx.  28 min

Reservoir 100 mL

The table shows the time it takes to reach the set temperature with specific Reservoir Rack modules and reaction vessels, if the user has set the set temperature in the software.

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8.2 Adapters

The following adapters are available for the epMotion:

• Height adapter

•Thermoadapter

8.2.1 Height adapter

Abb. 8-13: Height adapter

Fig. 8-13: Height adapter

The height adapters are used to offset the various heights of the labware. The tool holder does not need to offset these height differences and remains at one height. The shorter paths of this tool holder allows the application to run more quickly.

The height of the adapter is listed on the adapter. The combined height of the labware and the adapter may not exceed 126 mm.

Height adapters are not transported with the gripper.

Tab. 8-3: Available height adapters

Adapters Use with

40 mm • epT.I.P.S. Motion racks 50 µL and 300 µL in size

55 mm • DWP plates

• 400 mL reservoirs

• Thermoblock with PCR plates

85 mm • Almost all MTP plates with 6 – 384 wells

• Almost all PCR plates with 96 and 384 wells

• PCR thermoblock with PCR plates

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8.2.2 Vac holder vacuum holder

Abb. 8-14: Vac holder vacuum holder

Fig. 8-14: Vac holder vacuum holder

The vacuum holder is an adapter. The vacuum holder is used to store the vacuum frame on the worktable.

The vacuum holder is placed on location C4 of the worktable.

8.2.3 Thermoadapter

Abb. 8-15: Thermoadapter DWP 96

Fig. 8-15: Thermoadapter DWP 96

Thermoadapters are adapters for plates that can be tempered. A thermoadapter can be tempered on the thermal module. Thermoadapters can be placed on the worktable with or without plates.

Thermoadapters will not be transported using the gripper. The gripper can remove a plate from the thermoadapter or place it on the thermoadapter.

Thermoadapters are available for deepwell plates and PCR plates.

Labware accessories

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8.2.4 Thermoadapter LC samples

Abb. 8-16: Thermoadapter LC samples

Fig. 8-16: Thermoadapter LC samples

The Thermoadapter LC samples is a tube holder for automatically filling MagNA Pure LC Sample Cartridges. Thermoadapters and cartridges are a fixed combination for the epMotion.

The Thermoadapter LC Samples can be tempered up to 70 °C.

8.2.5 Thermoadapter Frosty

Abb. 8-17: Thermoadapter Frosty

Fig. 8-17: Thermoadapter Frosty

The Thermoadapter Frosty cools samples.

The Thermoadapter Frosty is a modified height adapter which includes a PCR-Cooler. A PCR plate is placed on the Thermoadapter Frosty. The PCR-Cooler cools the samples in this plate. The Thermoadapter Frosty can only be used with skirted PCR plates.

Preparing and equipping the Thermoadapter Frosty

1. Cool the PCR-Cooler by placing the PCR-Cooler in the lab refrigerator with the upper side down.

2. Insert the PCR-Cooler in the Thermoadapter Frosty.

3. Place a PCR plate on the Thermoadapter Frosty.

The PCR-Cooler changes color if a temperature of 7 °C is exceeded. The color changes from purple to pink or from dark blue to light blue. The color value in the recesses of the PCR-Cooler is a decisive factor for cooling samples.

8.3 Thermoblocks and Thermoadapters for PCR plates

PCRPCR

ThermoadapterThermoadapter

384384

PCRPCR

ThermoblockThermoblock

384384

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Abb. 8-18: PCR thermoblock 384

Fig. 8-18: PCR thermoblock 384

Abb. 8-19: Thermoblock PCR 96

Fig. 8-19: Thermoblock PCR 96

A thermoblock consists of a thermoadapter and a removable plate. For the epMotion, the Thermoblock and the removable plate form a unit.

Thermoblocks can be tempered on a thermal unit.

Store the thermoblock in the laboratory refrigerator to cool it.

The Thermoblock is transported by the gripper with the plate. The Thermoblock has recesses for the gripper.

Abb. 8-20: Thermoblock and thermoadapter comparison

Fig. 8-20: Thermoblock and thermoadapter comparison

The thermoadapter and thermoblock have different bar lengths.

Thermoblock Equipping Filling volume per vessel Heating period from 

0°C to - 10°C

PCR 96 96-well twin.tec PCR plate 150 µL ~ 14 min

PCR 384 384-well twin.tec PCR plate 25 µL ~ 10 min

8.3.1 Equipping the thermoblock with 0.2 mL vessels

Abb. 8-21: Tube lid rotated 45° to the surface of the thermoblock

Fig. 8-21: Tube lid rotated 45° to the surface of the thermoblock

 Arrange the vessels in columns. Leave every other column open for the tube lid.

A maximum of 48 vessels with attached lids can be positioned in the thermoblock.

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8.4 Waste container

8.4.1 Waste container

Abb. 8-22: Waste container

Fig. 8-22: Waste container

The waste container can hold up to 300 pipette tips up to 1000 µL in size.

8.4.1.1 Placing the disposal bag in the waste container

Use a disposal bag to protect the waste container from contamination.

Prerequisites

• Disposal bag

•Clamping ring

1. Place the disposal bag in the waste container

2. Fasten the disposal bag with the clamping ring.

3. Tightly pull the edge of the disposal bag downward.

The dispensing tool and rack access will not be impeded.

8.4.2 Waste funnel

The waste funnel holds large quantities of pipette tips.

If you would like to use a waste funnel, the epMotion must stand on work surface adapters. Contact Eppendorf AG before using a waste funnel.

8.4.3 LiquidWasteTub with lid

WARNING! Danger due to contamination of the LiquidWasteTub.

The outer surface of the LiquidWasteTub may become contaminated by pipette tips in the waste container.

 Wear personal protective equipment.  Decontaminate the outer surface of the LiquidWasteTub.

Abb. 8-23: LiquidWasteTub with lid

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1 2

Fig. 8-23: LiquidWasteTub with lid

1 Opening for dispensing tool

2 Opening for level detection

3Lid

4LiquidWasteTub

The LiquidWasteTub is a container for liquid waste and is hung in the waste container. The LiquidWasteTub has a working volume of 115 mL.

The optical sensor detects if the LiquidWasteTub is present. The optical sensor also detects the filling level of the LiquidWasteTub.

The lid of the LiquidWasteTub prevents contamination from liquid waste splashes. The lid can only be used with single-channel dispensing tools. If you are working with multi-channel dispensing tools, remove the lid from the LiquidWasteTub.

The LiquidWasteTub can be reused. Decontamination and cleaning (see Decontamination and cleaning on p. 97).

The equipping of the waste container with the LiquidWasteTub must be defined in the software.

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8.4.3.1 Using the LiquidWasteTub

NOTICE! Risk of collisions due to loose LiquidWasteTub.

If the LiquidWasteTub is not properly seated in the waste container, the dispensing tool may collide with the LiquidWasteTub when the tips are ejected.

 Always use the waste container with the clamping ring.

1. Place the disposal bag on the edge of the waste container (see p. 84).

2. Place the LiquidWasteTub on the edge of the waste container so that the tab of the LiquidWasteTub protrudes over the waste container clamping ring.

3. Place the lid on the LiquidWasteTub, if required.

4. Insert the waste container in the epMotion in such a way that the LiquidWasteTub is on the right-hand side.

Operation

epMotion

English (EN)

9 Operation

9.1 First steps

This chapter describes how to operate the epMotion and how to execute applications.

 Information on how to create applications is available in the software operating manual.

9.1.1 Switching on the epMotion

Prerequisites

• The epMotion has been installed and commissioned by skilled personnel authorized by Eppendorf.

1. Close the front hood.

2. Switch on the device at the mains power switch.

3. Switch on the MultiCon.

4. Start epBlue.

• The carrier travels to the home position.

• The epMotion is ready for operation.

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9.1.2 Using the MultiCon

You can find a detailed description of the MultiCon in the software operating manual.

9.1.2.1 Touch-sensitive screen (touch panel)

 Touch the screen with your finger.

You can also wear thin rubber gloves when operating the MultiCon.

9.1.2.2 Mouse

You can either use a mouse only or use it in addition to the touch-sensitive MultiCon screen.

 Connect the USB mouse to one of the USB ports.

The mouse arrow will appear on the screen as soon as the MultiCon has recognized the mouse.

9.1.2.3 Keyboard

You can either use a keyboard only or use it in addition to the touch-sensitive MultiCon screen.

 Connect the USB keyboard to one of the USB ports.

Operation

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9.1.3 Using the epMotion

The epMotion workflow consists of the following steps.

1. Create an application (refer to the epMotion software operating manual).

2. Equip the worktable (see p. 88).

3. Execute the application (see p. 93).

4. Complete your work (see p. 96).

Step 1 is described in the software operating manual. Steps 2 to 4 are described in the following sections.

9.2 Loading the worktable

To prepare the epMotion for an application, perform the following steps:

• Display the loading in the software

• Positioning tools

• epT.I.P.S. Motion

• Position the waste container

•Position labware

9.2.1 Display the loading

NOTICE! Tool collisions with incorrectly positioned labware.

Labware must be positioned on the epMotion worktable as defined in the application. Tools and labware become damaged in a collision. A collision can lead to sample loss.

 Check the positioning of the labware before starting the application.

To display the loading of the worktable, proceed as follows.

1. Open the application.

2. Select the area or the Run (refer to the epMotion software operating manual) step.

Operation

epMotion 5075

5075 v, vt

epMotion 5075

5075 t, vt, m

max. 2× TM1, TM2

epMotion 5075

5075 l, v

max. 3× TM1, TM2, TM3

epMotion 5075

5075 t, vt, m

TM 1

TM 2

TM 3

Waste

T0 T1

A3 A4 A5

B3 B4 B5B2B1B0

VAC

epT.I.P.S.

Adapter

epMotion 5075

5075 m

PrepRack

ReservoirRack

epMotion 5075

5075 v, vt

Vac Holder

epT.I.P.S.

Rack 3

epMotion 5075

5075 t, m

C1 C2 C3 C4 C5

TMX

Vac Lid

300mL/400mL

Liquid Waste Tub

5050

MagSep

epMotion

English (EN)

5075

The epBlue worktable displays the required equipping of the epMotion worktable graphically.

3. Equip the epMotion worktable as displayed.

Details on equipping can be found in the following chapters.

9.2.2 Positioning tools on the worktable

NOTICE! Damage to the gold contacts from handling.

The tool is damaged if the gold contacts on the tool become damaged or contaminated.

 Do not touch the gold contacts.

9.2.2.1 Dispensing tools

4 dispensing tools can be positioned on the worktable.

When the application is started, epMotion checks if the required tools are available. You can choose the order of the dispensing tools freely.

 Place the dispensing tool with the gold contact at the rear right of one of the T1 to T4 holders.

Operation

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9.2.2.2 Gripper

The gripper is required for applications in which labware is transported.

CAUTION! Risk of injury from mandrels on the gripper arms.

Each gripper arm has 2 sharp mandrels on the inside.

 Handle the gripper from above.  Do not touch the gripper arms on the inside.

Mounting the gripper on the holder

Abb. 9-1: Open (1) and close (2) the gripper arms.

1 2

Fig. 9-1: Open (1) and close (2) the gripper arms.

1Gripper arms

2 Pin for holding the gripper arms

3Holder

4Pins

1. Position the holder so that the pin is on the right-hand side.

2. Set the gripper down.

The gripper mandrels must engage in the bores of the holder. The pin must engage in the finger hole on the inside of the right gripper arm.

Positioning the gripper on the worktable

3. Place the gripper and holder at the T0 location.

4. Position the gripper and holder with the gold contacts at the rear.

9.2.3 Positioning the epT.I.P.S. Motion on the worktable

Observe the instructions for use for the epT.I.P.S. Motion Racks and epT.I.P.S. Motion Reloads.

NOTICE! Dispensing error due to incorrect handling of pipette tips.

Tips become deformed and change size during autoclaving.

 Do not autoclave the pipette tips. Use tips with the sterile specification, if required.  Do not stack any racks that contain pipette tips.

NOTICE! Incorrect stock detection due to missing pipette tips.

The optical sensor checks the start position and end position of the pipette tips in the rack. The optical sensor does not check if pipette tips are missing in the center.

 Do not remove any pipette tips from the rack.

NOTICE! Contamination due to using incorrect pipette tips.

The optical sensor does not detect if the pipette tips on the worktable are intended for single use (epT.I.P.S. Motion Rack, epT.I.P.S. Motion Reload) or multiple use (epT.I.P.S. Motion SafeRack).

Operation

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 If the pipette tips will be used several times, equip the entire worktable with epT.I.P.S.

Motion SafeRacks.

The optical sensor detects the type of pipette tips using the coding on the tray. The epMotion removes the pipette tips from the rack column by column. Additional information is available on the epT.I.P.S (see p. 66)

1. When using epT.I.P.S. Motion Reloads, place the tray on the tip holder.

2. Place the rack or tip holder on the worktable location with the label pointing toward the front.

3. Remove the lid.

9.2.4 Positioning the waste container on the worktable

1. Place the disposal bag in the waste container (see p. 84).

2. If required, insert the Liquid Waste Tub in the waste container (see p. 86).

3. Place the waste container in the epMotion.

Operation

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9.2.5 Positioning labware on the worktable

NOTICE! Collision or dispensing error due to uneven and tilted labware.

Labware must be positioned evenly on the worktable.

 Do not tilt the labware.  Place warped PCR plates on a thermoadapter or thermoblock.

NOTICE! Collision with tube lids.

If the lid blocks the tube opening, it may collide with the dispensing tool.

 Position the tube lid so the tube opening is not covered.

You can find information on special positioning features in the Labware chapter (see Labware accessories on p. 71).

9.2.5.1 Tubes

1. Open the tubes.

2. Place the tubes vertically in the racks.

Place tubes with lids in racks with lid fixture only.

3. Lock the attached lids in the lid fixtures on the rack.

9.2.5.2 Reservoir Rack modules

 Place the ReservoirRack Module in the ReservoirRack (see p. 76).

9.2.5.3 Plates, racks and adapters

The labware may not have any play at the epMotion worktable location.

1. Align the labware.

• Align the racks and adapters on the worktable in such a way that the writing faces the user.

• Align the plates on the worktable in such a way that position A1 of the plate is located at the rear on

the left.

Plates, racks, epT.I.P.S Motion racks and Thermoadapters

2. Position the labware in such a way that the spring clip holder pushes the labware against the positioning pins. The labware must touch the positioning pins of the location.

3. If required, tension the spring clip holder by hand.

Height adapter, Tip Holder und Reservoir Rack

4. Place the height adapter, Tip Holder and Reservoir Rack over the positioning pins.

9.3 Executing an application

Operation

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Details on starting and controlling applications can be found in the software operating manual.

9.3.1 Performing a test run

If you are executing an application for the first time, perform a test run.

Perform a test run with a liquid which has physical properties that are similar to the liquid for the application.

Check the application as follows:

1. Fill the labware with the correct dispensing quantity of demineralized water or a different test liquid.

2. Equip the epMotion worktable with labware and tools.

3. Activate the optical sensor.

4. Fully execute the application.

5. Check to see if the application was executed without errors.

• The dispensing is precise and correct.

• No liquid sprays out of the reaction vessels. Contamination is unlikely.

Additional information on optimizing the dispensing can be found in the software operating manual.

Operation

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9.3.2 Starting the application

NOTICE! Faulty level detection due to air bubbles.

If there are air bubbles in the vessels, the optical sensor reads the level incorrectly.

 Check to see if there are air bubbles in the vessels before starting the application.  To remove air bubbles, carefully tap the labware on the work surface.

Prerequisites

• Application is selected.

• Worktable is equipped (see p. 88).

1. Start the application.

2. Select the optical sensor settings for this run.

The following functions of the optical sensor can be activated:

• Liquid detection

Determines the filling level for positions of labware, which has the Liquid detection option activated.

• Tip detection

Checks to see if the pipette tips defined in the application are available. Determines the quantity and position of tips in the rack. Works only with epT.I.P.S. Motion in TipHolders and Racks from Eppendorf.

• Location detection

Detects the labware coding. Checks to see if the labware on the epMotion worktable matches the labware on the epBlue worktable.

A detailed description of the optical sensor and its functions can be found in the software operating manual.

3. For applications with a variable sample quantity, enter the number of samples.

4. If the level detection is deactivated, enter the filling volume of the vessels in µL.

• The epMotion checks if the required tools are available.

• If necessary, the optical sensor checks the labware and levels.

• The application is executed. The software displays the status of the application.

9.3.3 Controlling the application run

CAUTION! Burns from hot metal surfaces.

The metal surface heats up during operation. The software displays the current temperature of the metal surface.

 Wait until the metal surface has cooled down completely.

CAUTION! Risk of injury due to carrier movement.

The carrier may still be moving when the front hood of the epMotion is opened.

 Wait until the carrier stops moving before reaching into the device.

Operation

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9.3.3.1 Stopping the application immediately

To stop the application immediately, e.g., at risk of collision, proceed as follows:

 Open the front hood.

• The application will be stopped immediately.

 You can continue or abort the application.

9.3.3.2 Interrupting the application

 Stop the application using the Stop application button (refer to the epMotion software operating

manual).

The current movement will be ended. Then the application stops.

 You can continue or abort the application.

9.3.3.3 Continuing the application

5075

Prerequisites

• The equipping of the worktable has not been changed.

• The levels of the vessels has not been changed.

• The carrier has not been moved by hand.

1. Close the safety screen.

2. Continue the application using the Continue application button (refer to the epMotion software operating manual).

9.3.3.4 Aborting the application

NOTICE! Contamination due to multiple use of pipette tips.

When you abort an application with multiple use of pipette tips, the epT.I.P.S. Motion SafeRacks contain used pipette tips. The epMotion does not detect which pipette tips have already been used.

 Dispose of epT.I.P.S. Motion SafeRacks immediately after the application has been

aborted.

An application cannot be continued after it has been aborted.

Prerequisites

• The application was stopped or interrupted.

 Abort the application using the Abort application button (refer to the epMotion software operating

manual).

• The application will be aborted.

• The tool holder returns the tool to its original location.

• The carrier travels to the home position.

• The epMotion is ready for operation.

 You can start a new application.

Operation

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9.4 Completing your work

9.4.1 Cleaning up the worktable

WARNING! Danger due to flammable and infectious liquids in the waste container.

Pipette tips in the waste container may contain flammable or infectious liquids.

 Wear personal protective equipment.  Handle the pipette tips and sample materials from the waste container in accordance with

the material safety data sheets, safety regulations and laboratory guidelines.

WARNING! Danger due to contamination of the LiquidWasteTub.

The outer surface of the LiquidWasteTub may become contaminated by pipette tips in the waste container.

 Wear personal protective equipment.  Decontaminate the outer surface of the LiquidWasteTub.

Complete the following steps after the end of the application.

1. Switch off the HEPA filter, if applicable.

2. Seal the vessels.

3. Remove the labware from the worktable.

4. Empty the waste container.

5. If necessary, decontaminate the device and labware (see Decontamination and cleaning on p. 97).

6. Use a paper towel to soak up the condensation water on the thermal module or thermomixer.

9.4.2 Switching off the epMotion

Proceed as follows.

1. Exit epBlue (refer to the epMotion software operating manual).

2. Switch off the MultiCon.

3. Switch off the epMotion using the mains power switch.

10 Maintenance

10.1 Decontamination and cleaning

WARNING! Danger due to flammable and infectious liquids in the waste container.

Pipette tips in the waste container may contain flammable or infectious liquids.

 Wear personal protective equipment.  Handle the pipette tips and sample materials from the waste container in accordance with

the material safety data sheets, safety regulations and laboratory guidelines.

WARNING! Danger due to contamination of the LiquidWasteTub.

The outer surface of the LiquidWasteTub may become contaminated by pipette tips in the waste container.

 Wear personal protective equipment.  Decontaminate the outer surface of the LiquidWasteTub.

Maintenance

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CAUTION! Risk of injury from mandrels on the gripper arms.

Each gripper arm has 2 sharp mandrels on the inside.

 Handle the gripper from above.  Do not touch the gripper arms on the inside.

NOTICE! Material damage from corroding cleaning agents.

Cleaning and decontamination agents may contain corroding substances. Metal surfaces on the epMotion become damaged by corrosion.

 Use the cleaning agents and disinfectants specified in the operating manual.

The device is cleaned as described in the Decontamination section.

Maintenance

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English (EN)

Tab. 10-1: Decontamination methods and decontamination agents

Decontamination method Decontamination agent For use on

Wipe decontamination 70% (v/v) ethanol Worktable

Waste container LiquidWasteTub Housing Work surface adapter Barcode reader Vacuum unit Lid, thermo lid and sealing mat of the vacuum unit Labware Tools

3%–4% sodium hypochlorite solution

Waste container LiquidWasteTub Dispensing Tools Gripper Vacuum unit Lid, thermo lid and sealing mat of the vacuum unit Elution plate adapter Racks Thermoblocks Thermoadapter Height adapter TipHolder

Spray decontamination outside the device

70% (v/v) ethanol Waste box

LiquidWasteTub Racks Thermoblocks Thermoadapter Height adapter TipHolder

UV irradiation UV lamp of the CleanCap

1 irradiation cycle lasts 15 min

Worktable Thermomixer Thermal module

Autoclaving 121 °C

100 kPa overpressure 20 min

Waste box LiquidWasteTub Dispensing Tools Gripper Racks Thermoblocks Thermoadapter Height adapter TipHolder Lid, thermo lid and sealing mat of the vacuum unit Elution plate adapter

10.1.1 Carrying out wipe decontamination

Prerequisites

• Decontamination agent

• Deionized water

• Lint-free cloths

• Cotton swabs

Cleaning the device

1. Switch off the device and disconnect it from the mains/power line.

2. Moisten the lint-free cloth and cotton swab with decontamination agent.

3. Clean the surface with a lint-free cloth. Clean hard to access surfaces with cotton swabs.

The surfaces are dampened with a decontamination agent.

4. Allow the decontamination agent to take effect.

5. Wipe the decontamination agent with deionized water.

6. Allow the surfaces to dry.

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Cleaning the dispensing tools

7. Pull of the ejector from the single-channel dispensing tool.

Abb. 10-1: Removing the single-channel dispensing tool ejector

Fig. 10-1: Removing the single-channel dispensing tool ejector

8. Clean and decontaminate the dispensing tools.

The wipe decontamination of the dispensing tools is identical to the wipe decontamination of the device.

100

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10.1.2 Carrying out spray decontamination

NOTICE! Damage on the device due to ingress of liquids by spray decontamination.

 Do not perform spray decontamination in and on the device. Use the decontamination

methods provided in the operating manual.

 When you labware and tools, remove labware and tools from the device beforehand.

Prerequisites

• Decontamination agent in spray bottle

• Deionized water

1. Remove Labware and tools from the device.

2. Spray the surfaces with decontamination agent.

3. Allow the decontamination agent to take effect.

4. Wipe the decontamination agent with deionized water.

5. Allow the surfaces to dry.

10.1.3 Carrying out UV irradiation

A UV irradiation may only be carried out for an epMotion with CleanCap.

 Prepare the device and carry out the irradiation as described (see UV lamp on p. 48).

10.1.4 Autoclaving

NOTICE! Damage on the tool due to autoclaving.

The tool will be damaged if the temperature in the autoclave is too high.

 Make sure the temperature in the autoclave is 121 °C – 126 °C.  Make sure the tools do not touch the wall of the autoclave.

Prerequisites

• The temperature in the autoclave is 121 °C.

• 100 kPa overpressure is present in the autoclave.

• Chemical decontamination agents are removed completely.

1. Autoclave tools and labware for 20 min.

2. Rinse the tools and labware with deionized water.

3. Let the tools and labware dry.

Eppendorf 5075 User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

1 Operating instructions

1.1 Using this manual

1.2 Danger symbols and danger levels

1.2.1 Danger symbols

1.2.2 Danger levels

1.3 Symbols used

1.4 Abbreviations used

1.5 Glossary

1.6 Revision history

2 Product description

2.1 Main illustration

2.1.1 epMotion 5075

2.1.2 Interfaces

2.1.3 MultiCon

2.2 Delivery package

2.2.1 epMotion 5075l and epMotion 5075lg

2.2.2 epMotion 5075t and epMotion 5075tg

2.2.3 epMotion 5075v and epMotion 5075vg

2.2.4 epMotion 5075vt and epMotion 5075vtg

2.2.5 epMotion 5075m and epMotion 5075mg

2.2.6 Accessories for all versions of the epMotion

2.2.7 Accessories for epMotion with epBlue ID

2.2.8 Accessories for epMotion with epBlue GxP

2.3 Features

2.3.1 Function

2.3.2 Gripper

2.3.3 Thermal module

2.3.4 Thermomixer

2.3.5 Magnetic separation

2.3.6 Vacuum unit

2.3.7 CleanCap

3Safety

3.1 Intended use

3.1.1 Intended use of the epMotion 5075

3.2 User profile

3.3 Information on product liability

3.4 Warnings for intended use

3.5 Hazard symbols and safety devices on the device

4 Installation

4.1 Selecting the location

4.2 Installing the device

5Hardware

5.1 Worktable

5.1.1 Layout of the epMotion 5075I worktable

5.1.2 Layout of the epMotion 5075t worktable

5.1.3 Layout of the epMotion 5075v worktable

5.1.4 Layout of the epMotion 5075vt worktable

5.1.5 Layout of the epMotion 5075m worktable

5.2 Optical sensor

5.3 Thermal module

5.3.1 Safety notes

5.3.2 Configuration

5.3.3 Equipping the thermal module

5.4 Thermomixer

5.4.1 Safety notes

5.4.2 Configuration

5.4.3 Equipping the thermomixer

5.5 Magnet finger module

5.5.1 Configuration

5.5.2 Equipping the magnet finger module

5.6 Vacuum unit

5.6.1 Vac Frame vacuum frame

5.6.2 VAC Lid

5.6.3 Silicone mat for VAC Lid

5.6.4 Vac Thermo Lid

5.6.5 Elution plate adapter

5.6.6 Loading the vacuum unit

5.7 CleanCap

5.7.1 HEPA filter

5.7.2 UV lamp

5.8 Barcode reader

5.9 Tools

5.9.1 Dispensing Tools

5.9.2 Gripper

6 Dispensing liquids

6.1 Basic procedure for dispensing