Eppendorf 5070 User Manual
n
E
Register your instrument!
www.eppendorf.com/myeppendorf
ual070
N)
manual
®
epMotion
5070
Hardware manual
©
Copyright
2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior
permission of the copyright owner.
Trademarks
Eppendorf
®
, the Epppendorf logo, epMotion®, Eppendorf Tubes ®, epT.I.P.S.® and are registered
trademarks of Eppendorf AG.
®
LightCycler
and MagNA Pure® are registered trademarks of Roche Diagnostics.
epMotion optical sensor U.S. Pat. No. 6,819,437
Registered trademarks are not marked in all cases with
®
or ™ in this manual.
The software of the device (firmware) contains open source software. License information is available on
request from Eppendorf AG.
5070 900.850 -00/062013
Table of contents
epMotion
®
5070
English (EN)
Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.5 Glossary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.6 Revision history. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.1.1 epMotion 5070 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.1.2 epMotion 5070f . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.1.3 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2.1.4 EasyCon. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
2.2 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
2.3.1 Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
2.3.2 Cleanbench . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3
3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.1.1 Intended use of the epMotion 5070 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.1.2 Intended use of the epMotion 5070f . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
3.5 Hazard symbols and safety devices on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.2 Installing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5 Hardware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.1 Worktable for epMotion 5070 and 5070f . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.2 Optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5.3 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.3.1 Dispensing Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Table of contents
®
4
epMotion
5070
English (EN)
6 Dispensing liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
6.1 Basic procedure for dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
6.1.1 Operating principle of dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
6.1.2 Liquid aspiration up to the remaining volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
6.1.3 Bottom tolerance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
6.1.4 Transporting and dispensing liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
6.2 Dispensing modes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
6.2.1 Dispensing mode: Pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
6.2.2 Dispensing mode: Multidispense . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
6.2.3 Mixing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
6.3 Special features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
6.3.1 Liquid aspiration from bottom of vessel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
6.3.2 Liquid aspiration from high vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
6.3.3 Liquid dispensing at the height of the vessel edge. . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
7 Labware – vessels, plates and pipette tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
7.1 Tubes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
7.2 Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
7.2.1 Deepwell plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
7.2.2 Microplates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
7.2.3 PCR plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
7.2.4 Tube plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
7.3 Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
7.4 epT.I.P.S. Motion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
7.4.1 epT.I.P.S. Motion Racks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
7.4.2 epT.I.P.S. Motion Reloads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
7.4.3 Tip holder for epT.I.P.S. Motion Reloads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
7.4.4 epT.I.P.S. Motion SafeRacks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
8 Labware accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
8.1 Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
8.1.1 Racks for 24 vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
8.1.2 Rack for 96 vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
8.1.3 Rack LC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
8.1.4 Thermorack and Rack 0.5/1.5/2.0 mL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
8.1.5 Reservoir Rack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
8.1.6 Reservoir Rack modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
8.2 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
8.2.1 Height adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
8.2.2 Thermoadapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
8.2.3 Thermoadapter LC samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
8.2.4 Thermoadapter Frosty. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
8.3 Thermoblocks and Thermoadapters for PCR plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
8.3.1 Equipping the thermoblock with 0.2 mL vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
8.4 Waste container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
8.4.1 Waste container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
8.4.2 LiquidWasteTub with lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Table of contents
®
epMotion
5070
English (EN)
9 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
9.1 First steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
9.1.1 Switching on the epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
9.1.2 Using the EasyCon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
9.1.3 Using the epMotion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
9.2 Loading the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
9.2.1 Display the loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
9.2.2 Positioning tools on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
9.2.3 Positioning the epT.I.P.S. Motion on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
9.2.4 Positioning the waste container on the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
9.2.5 Positioning labware on the worktable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
9.3 Executing an application. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
9.3.1 Performing a test run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
9.3.2 Starting the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
9.3.3 Controlling the application run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
9.4 Completing your work. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
9.4.1 Cleaning up the worktable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
9.4.2 Switching off the epMotion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
5
10 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
10.1 Decontamination and cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
10.1.1 Carrying out wipe decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
10.1.2 Carrying out spray decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
10.1.3 Autoclaving . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
10.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
10.3 Servicing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
10.4 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
10.5 Carrying out firmware updates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
10.6 Checking the dispensing volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
10.7 Maintaining the dispensing tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
10.7.1 Exchanging the sealing rings on the eight-channel dispensing tool . . . . . . . . . . . . . . 76
10.8 Customer service functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
11 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
11.1 Error search . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
11.2 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
11.2.1 Optical sensor errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
11.2.2 Dispensing errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
11.2.3 Software errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
12 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
12.1 Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
12.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
12.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Table of contents
®
6
epMotion
5070
English (EN)
13 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
13.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
13.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
13.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
13.4 Noise level. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
13.5 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
13.6 EasyCon. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
13.7 Additional specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
13.7.1 Optical sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
13.7.2 Carrier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
13.8 Dispensing tool errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
13.8.1 Pipetting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
13.8.2 Dispensing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
13.9 Test conditions for dispensing tool errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
14 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
14.1 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
14.2 epT.I.P.S. Motion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
14.3 Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
14.4 Reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
14.5 Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
14.6 Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
14.7 Thermoblocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
14.8 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
Operating instructions
epMotion
English (EN)
1 Operating instructions
1.1 Using this manual
Your epMotion operating manual consists of hardware instructions and software instructions. Short
instructions are available for optional software enhancements.
The operating manual is part of the product.
®
5070
7
The current version of the operating manual can be found on our webpage: www.eppendorf.com
.
Read the operating manual in full before using the device.
Store the operating manual at an easily accessible location.
The device may only be transferred with the operating manual.
If the operating manual is lost, replace it immediately. Please contact Eppendorf AG for further details.
1.2 Danger symbols and danger levels
The safety instructions in this manual appear with the following danger symbols and danger levels:
1.2.1 Danger symbols
Biohazard Explosion
Electric shock Heavy loads
Cuts Hot surface
Hazard point Material damage
1.2.2 Danger levels
DANGER Will lead to severe injuries or death.
WARN ING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
Operating instructions
epMotion
8
English (EN)
®
5070
1.3 Symbols used
Depiction Meaning
1.
2.
Actions without a specified order
• List:
Text Display or software texts
1.4 Abbreviations used
DNA
Deoxyribonucleic acid (DNA)
Actions in the specified order
Additional information
DWP
Deepwell plate
epT.I.P.S.
eppendorf Totally Integrated Pipetting System
LH
Liquid handling
MTP
Micro test plate
PCR
Polymerase chain reaction
PDF
Portable Document Format
USB
Universal Serial Bus
Operating instructions
epMotion
®
5070
English (EN)
1.5 Glossary
A
Application
Programs for a specific application. An application includes the procedure and the equipping of the
worktable.
C
Cleanbench
Class 2 safety cabinet for personal security and product protection. A laminar air flow prevents germs from
entering the cabinet from the ambience. The air flow also prevents aerosols that contain microorganisms
from escaping the cabinet.
D
9
Destination labware
Labware that liquid is dispensed into during the application. Destination labware refers to a plate or rack.
Destination position
Position that liquid is dispensed into during the application.
Destination vessel
The vessel into which liquid is dispensed during the application.
Dispensing tool
Tool that aspirates and dispenses liquid. Single-channel dispensing tools and eight-channel dispensing
tools are available for various volume ranges.
E
EasyCon
epMotion control panel. You can control your epMotion using the control panel and epBlue software.
epBlue
Software for creating and administering applications and labware. The epMotion is controlled using the
software.
Eppendorf Quality
Eppendorf Quality is an Eppendorf AG purity grade for consumables. Eppendorf Quality meets the
requirements for standard products, e.g., precision, accuracy, wetting behavior and tightness.
epT.I.P.S.® Motion
epMotion pipette tips. Only epT.I.P.S.® Motion can be used on the epMotion for all features. epT.I.P.S.®
Motions are available with or without a filter.
10
Operating instructions
®
epMotion
5070
English (EN)
F
Filling volume
Maximum liquid volume of a labware. The epMotion uses the gripper to transport the labware up to the
filling volume. The epMotion aspirates liquid from the labware up to the filling volume. The filling volume is
higher than the working volume.
H
Height adapter
Adapter for low labware. The height differences among the labware will be offset to decrease the tool
holder paths, thereby decreasing the run time of the application as well.
I
Intermediate labware
Labware that is used to dispense and aspirate liquids during the application.
Intermediate position
Position that is used to dispense and aspirate liquids during the application.
Intermediate vessel
Vessel that is used to dispense and aspirate liquids during the application.
L
Labware
Racks, plates, tips, etc. which are placed on the worktable.
Location
Area on the worktable where the labware is placed.
P
PCR clean
PCR clean is an Eppendorf AG purity grade for consumables. PCR clean meets the requirements for
standard products, e.g., precision, accuracy, wetting behavior, tightness. PCR clean also meets the
requirements with regard to absence of human DNA, DNase, RNase and PCR inhibitors.
Consumables with the PCR clean purity grade are controlled and certified by an external laboratory.
Certificates are available for downloading from our webpage www.eppendorf.com
.
Procedure
Sequence of commands that are executed one after the other. Part of an application.
R
Rack
Mount for tubes or pipette tips.
Operating instructions
®
epMotion
5070
English (EN)
Random error
Precision. Standard deviation of the average value of the dispensed volumes.
Remaining volume
Volume that cannot be aspirated from a vessel. The distance from the pipette tip to the vessel bottom,
defined in the software, and the defined immersion depth of the pipette tip in the liquid, must be observed.
Therefore, the pipette tip cannot aspirate the volume. The remaining volume depends on the vessel
geometry.
Reservoir
Reservoirs are used to hold reagents. Reservoirs are hung in a Reservoir Rack or placed directly on the
worktable.
S
SafeRack
Rack with ep.T.I.P.S. Motion pipette tips. The SafeRack features a partition that prevents the contamination
of adjacent tips. Use the SafeRacks if you would like to use tips several times.
11
Source labware
Labware that liquid is aspirated from during the application. Source labware refers to a plate or rack.
Source position
Position that liquid is aspirated from during the application.
Source vessel
The vessel from which liquid is aspirated during the application.
Sterile
Sterile is an Eppendorf AG purity grade for consumables. Sterile meets the requirements for standard
products, e.g., precision, accuracy, wetting behavior, tightness. Sterile also meets the requirements with
regard to sterility and freedom from pyrogens.
Systematic error
Accuracy. Deviation of the average value of the dispensed volumes from the selected volume.
T
Thermoadapter
Heat-conductive adapter for holding plates.
Thermoblock
A thermoadapter that is permanently connected to a PCR plate or PCR tube.
Thermorack
Temperable rack for smaller vessels, e.g., Safe-Lock tubes for 0.5 mL, 1.5 mL or 2 mL.
12
Operating instructions
epMotion
English (EN)
V
Vessel
Tube or single well of a plate.
W
Work volume
Liquid volume of a labware. The epMotion fills a vessel with low levels of contamination up to the working
volume. The working volume is less than the filling volume.
Worktable
Work surface of the epMotion where labware and tools are placed. In the software, the epMotion worktable
is shown as the epBlue worktable.
®
5070
1.6 Revision history
Revision history for the hardware operating manuals for the epMotion 5070, order number 5070 900.850.
Version of the operating manual Date Changes
Version 00 June 2013 Created new
2 Product description
epMotion 5070
x
y
z
4
6
7
9
8
1
23
5
2.1 Main illustration
2.1.1 epMotion 5070
Abb. 2-1: Main illustrati on epMotion 5070
Product description
®
epMotion
5070
English (EN)
13
Fig. 2-1: Main illustration epMotion 5070
1Carrier
The carrier moves in X-direction, Y-direction and
Z-direction.
2 Tool holder
Holds dispensing tools.
3Optical sensor
Detects levels, tips and labware.
4 Mains power connection
5Interfaces
6Worktable
Work surface for tools and labware.
7EasyCon
Control panel used to control the epMotion.
8 Front hood
Safety device for protection from movable parts
and contamination.
9 Mains power switch
14
x
y
z
epMotion 5070
4
6
7
8
1
23
5
9
Product description
®
epMotion
5070
English (EN)
2.1.2 epMotion 5070f
Abb. 2-2: Main illustrati on epMotion 5070f
Fig. 2-2: Main illustration epMotion 5070f
1Carrier
The carrier moves in X-direction, Y-direction and
Z-direction.
2 Tool holder
Holds dispensing tools.
3Optical sensor
Detects levels, tips and labware.
4 Mains power connection
5Interfaces
6Worktable
Work surface for tools and labware.
7EasyCon
Control panel used to control the epMotion.
8 Mains power switch
9 Light barrier
The light reflectors are located on the inside of
the front screen of the Cleanbench.
2.1.3 Interfaces
Ethernet
USB
Abb. 2-3: Interfaces epMot ion 5070 and 5070f
Fig. 2-3: Interfaces epMotion 5070 and 5070f
Product description
®
epMotion
5070
English (EN)
15
Ethernet
Connection for the cable to the EasyCon
USB
Connection for a USB storage medium for
firmware updates.
Only devices which meet the requirements of IEC 950/EN 60950-1 (UL 1950) standards may be connected
to the interfaces.
16
Product description
®
epMotion
5070
English (EN)
2.1.4 EasyCon
Abb. 2-4: EasyCon
Fig. 2-4: EasyCon
The EasyCon is the control panel of the epMotion. The EasyCon has a touch-sensitive color display
(touchscreen). A key pad will be shown on the display for data entry.
Abb. 2-5: InterfacesEasyCon
1
2
Fig. 2-5: InterfacesEasyCon
1USB
Connections for mouse with a USB connection
2Ethernet
Connection for the epMotion cable
cable or USB storage medium
Only devices which meet the requirements of IEC 950/EN 60950-1 (UL 1950) standards may be connected
to the interfaces.
2.2 Delivery package
Product description
epMotion
®
5070
English (EN)
17
QuantityOrder no.
(International)
1 5070 000.263 100 V - 240 V, 50 / 60 Hz
or 1 5070 000.264 100 V - 240 V, 50 / 60 Hz
1
1 - With USB connection cable
1
1 - Compatible with the country where the order was placed or defined
1
1- Tool for transport securing device
1 5075 753.006 Waste box
1 5070 900.850 epMotion 5070
1 5075 901.250 software operating manual epBlue with EasyCon
5073 005.002
-
-
Description
Automated pipetting system epMotion 5070
main device, with EasyCon, accessories, installed software epBlue
Automated pipetting system epMotion 5070f
main device for cleanbench, with EasyCon, accessories,
installed software epBlue
EasyCon
Control panel
Mouse
Cable
For connecting the EasyCon to the epMotion
Mains power cable
USB cable
For connecting USB devices to the EasyCon
Operating Manual
Operating Manual
18
Product description
epMotion
English (EN)
®
5070
2.3 Features
The epMotion 5070 is available with different features. All available features are described in this manual.
2.3.1 Function
The epMotion 5070 is an automatic system for dispensing liquids using dispensing tools and ep.T.I.P.S.
Motion pipette tips.
The epMotion 5070 dispenses liquids in the volume range 1 µL – 1 000 µL.
The dispensing tool aspirates liquid from source vessels and dispenses this liquid in destination vessels.
The dispensing tool works according to the piston-stroke principle. For the epMotion, dispensing tools are
available for 3 different volume ranges.
The epMotion features an optical sensor. The sensor checks:
• Type and location of labware
• Quantity and position of pipette tips in the rack
• Filling level of vessels
You control the epMotion 5070 via the EasyCon control unit with the epBlue software.
You can use the epBlue software to define dispensing processes and compile them into an application. To
do so, you select source vessels and destination vessels, define the procedure and define the transfer
pattern.
2.3.2 Cleanbench
The epMotion 5070f must only be operated in a Cleanbench. The function corresponds to a epMotion 5070
without a Cleanbench.
Additional information on your epMotion is available at: www.eppendorf.com/automation
.
Safety
®
epMotion
English (EN)
5070
3Safety
3.1 Intended use
3.1.1 Intended use of the epMotion 5070
The device is intended for use in laboratories for research, development, and industrial and routine work,
as well as for training purposes. The areas of applications include – but are not limited to – the fields of life
sciences, biotechnology, chemistry and clinical research.
The automated pipetting system epMotion 5070 is designed for the monitoring of contamination-free,
precise and correct dispensing and transferring of liquids and to automatically check the combining of
liquids.
The autoclavable dispensing tools work in a volume range from 1 L to 1000 L.
The device meets the basic, essential requirements of the EC Directives and Standards that are listed in the
declaration of conformity, but the product is not registered with the FDA.
19
These automated pipetting systems are exclusively intended for use indoors and may only be used by
skilled personnel who have received adequate training.
3.1.2 Intended use of the epMotion 5070f
The device is intended for use in laboratories for research, development, and industrial and routine work,
as well as for training purposes. The areas of applications include – but are not limited to – the fields of life
sciences, biotechnology, chemistry and clinical research.
The automated pipetting system epMotion 5070f is designed for the monitoring of contamination-free,
precise and correct dispensing and transferring of liquids and to check the combining of liquids
automatically.
The autoclavable dispensing tools work in a volume range from 1 L to 1000 L.
The epMotion 5070f must only be operated in a Cleanbench.
The device meets the basic, essential requirements of the EC Directives and Standards that are listed in the
declaration of conformity, but the product is not registered with the FDA.
These automated pipetting systems are exclusively intended for use indoors and may only be used by
skilled personnel who have received adequate training.
3.2 User profile
This device may only be operated by skilled personnel.
The skilled personnel must have received training on this device. The training must have been conducted
by Eppendorf AG or an authorized partner of Eppendorf AG.
The skilled personnel must have read the operating manual carefully. The skilled personnel must have read
the operating manuals of all software components carefully.
20
Safety
epMotion
English (EN)
®
5070
3.3 Information on product liability
In the following cases, the designated protection of the device may be compromised. Liability for any
resulting property damage or personal injury is then transferred to the operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables which are not recommended by Eppendorf.
• The device is maintained or repaired by people not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
3.4 Warnings for intended use
DANGER! Risk of explosion.
Do not operate the device in areas where work is completed with explosive substances.
Do not use this device to process any explosive or highly reactive substances.
Do not use this device to process any substances which may generate an explosive
atmosphere.
WARNING! Lethal voltages inside the device.
Ensure that the housing is always closed and undamaged so that no parts inside the device
can be contacted by accident.
Do not remove the housing of the device.
Do not allow any liquids to penetrate the inside of the housing.
Do not allow the device to be opened by anyone except service personnel who have been
specifically authorized by Eppendorf.
WARNING! Electric shock due to damage to device or mains cable.
Only switch on the device if the device and mains cable are undamaged.
Only use devices that have been properly installed or repaired.
In case of danger, disconnect the device from the mains supply by pulling the power plug
from the device or the mains socket or, by using the isolating device intended for this
purpose (e.g. emergency stop switch in the laboratory).
WARNING! Risk from incorrect supply voltage
Only connect the device to voltage sources which correspond to the electrical
requirements on the name plate.
Only use sockets with a protective earth (PE) conductor and suitable power cable.
Safety
®
epMotion
English (EN)
WARNING! Damage to health due to infectious liquids and pathogenic germs.
5070
When handling infectious liquids and pathogenic germs, observe the national regulations,
the biological security level of your laboratory, the material safety data sheets, and the
manufacturer's application notes.
Wear personal protective equipment.
For full instructions regarding the handling of germs or biological material of risk group II
or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health
Organization, current edition of the Laboratory Biosafety Manual).
WARNING! Health hazard from skin contact with infectious substances.
Wear protective gloves.
WARNING! Danger due to flammable and infectious liquids in the waste container.
Pipette tips in the waste container may contain flammable or infectious liquids.
Wear personal protective equipment.
Handle the pipette tips and sample materials from the waste container in accordance with
the material safety data sheets, safety regulations and laboratory guidelines.
21
WARNING! Damage to health due to contaminated device and accessories.
Decontaminate the device and the accessories before storage and shipping.
CAUTION! Risk of injury due to carrier movement.
The carrier may still be moving when the front hood of the epMotion is opened.
Wait until the carrier stops moving before reaching into the device.
CAUTION! Cuts due to broken glass.
A damaged touchscreen leads to cuts on the hands.
Only use the EasyCon if it is not damaged.
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf may
impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or
accept any liability for damage resulting from the use of incorrect or non-recommended
accessories and spare parts, or from the improper use of such equipment.
Only use accessories and original spare parts recommended by Eppendorf.
NOTICE! Spilled liquid can cause damage to the device.
Switch off the device.
Disconnect the power plug.
Collect the spilled liquid. Observe the specifications for the liquid in the material safety
data sheets.
NOTICE! Size of disposables can change through autoclaving.
Do not use autoclaved disposable products in automated applications.
22
epMotio n 5 0 70
epMotio n 5 0 70
12
12
4
epMotion 5070 epMotion 5070f
Safety
®
epMotion
5070
English (EN)
3.5 Hazard symbols and safety devices on the device
This section explains the warning symbols on the epMotion and the location of the safety devices.
Abb. 3-1: Hazard symbols and safety devices on the epMotion 5070
Fig. 3-1: Hazard symbols and safety devices on the epMotion 5070
Tab. 3-1: Hazard symbols
1 WARNING
Observe the operating manual.
2 WARNING
The carrier may continue moving after the front panel has
been opened.
Wait until the carrier stops moving before reaching into
the device.
Tab. 3-2: Safety devices
4 The housing or the cleanbench protects the user from
contamination and moving parts.
Installation
®
epMotion
English (EN)
5070
4 Installation
4.1 Selecting the location
Information on ambient conditions, dimensions and weights can be found in the technical data (see Weight/
dimensions on p. 85).
Select the location for the device according to the following criteria:
Electrical connections
• Mains connection in accordance with the name plate.
• The device mains/power switch and cutting unit of the power system circuit (e.g., FI protective switch)
can be accessed during operation.
Location
• The ambient conditions match the specifications in the technical data.
• The location is well ventilated.
• The location is protected from direct sunlight.
• The location is not next to heat sources, such as heaters or drier compartments.
• There is an adequate amount of space available for the device. The minimum distances to other devices
and walls is 6 cm.
• The device can be safely and easily operated at this location.
23
Workplace
• The lab bench is permanently mounted.
• The lab bench is designed for the weight of the device.
• The lab bench has a horizontal, plane work surface.
• The lab bench has a non-slip surface.
• The lab bench is vibration-free.
• There are no vibrating devices on the lab bench.
Cleanbench
• The Cleanbench is 60 cm deep.
• The Cleanbench cables are routed along its side.
• The laminar airflow in the Cleanbench is not affected by the device.
• There are light reflectors on the front screen of the Cleanbench.
4.2 Installing the device
The epMotion may only be installed and commissioned by skilled personnel authorized by
Eppendorf.
Information on transport (see Transport on p. 83).
24
Installation
epMotion
®
English (EN)
5070
5Hardware
5.1 Worktable for epMotion 5070 and 5070f
Abb. 5-1: Worktable for epMotion
Fig. 5-1: Worktable for epMotion
Hardware
epMotion
English (EN)
®
5070
25
A1-B2
Locations for labware
T1-T2
Locations for dispensing tools
Waste
Location for waste container
26
Detector
Diaphragm
Z
Z
0
Imaging optics
Light source
Semi-transparent
mirror
Diaphragm
Hardware
®
epMotion
5070
English (EN)
5.2 Optical sensor
Abb. 5-2: Optical sensor principle
Fig. 5-2: Optical sensor principle
On the carrier, the optical sensor is located to the right of the tool holder.
The optical sensor records the intensity of the reflected light using a lateral infrared light source,
semi-transparent mirror, fixed lens and the procedure in z-direction. Height z
is defined as the point with
0
the highest intensity which enables the detection of the level in a vessel or the presence of labware.
The optical sensor can detect horizontal, plane surfaces. The surface of the liquid must be at an angle of
90° ± 3° to the optical axis.
The surfaces of liquids can be strongly bent by the vessel geometry or the physical properties of the liquid
or vessel. The optical sensor may not detect the level on strongly bent surfaces. If this is the case, the user
must enter the liquid volume.
The optical sensor has a detection limit for vessel levels. The detection limit depends on the vessel
geometry. Generally, levels of 3 mm or higher can be detected.
The following functions of the optical sensor can be activated:
• Liquid detection
Determines the filling level for positions of labware, which has the Liquid detection option activated.
• Tip detection
Checks to see if the pipette tips defined in the application are available.
Determines the quantity and position of tips in the rack.
Works only with epT.I.P.S. Motion in TipHolders and Racks from Eppendorf.
• Location detection
Detects the labware coding.
Checks to see if the labware on the epMotion worktable matches the labware on the epBlue worktable.
Information on setting the optical sensor, and a detailed description of all functions, can be
found in the software operating manual.
Hardware
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®
epMotion
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English (EN)
5.3 Tools
NOTICE! Damage to the gold contacts from handling.
The tool is damaged if the gold contacts on the tool become damaged or contaminated.
Do not touch the gold contacts.
The tool holder picks up a dispensing tool and puts it down again. The tool holder recognizes the tool from
its gold contacts.
5.3.1 Dispensing Tools
Abb. 5-3: Single-channel dispensing tool and eight-channel dispensing tool
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50
Fig. 5-3: Single-channel dispensing tool and eight-channel dispensing tool
The dispensing tools are piston-stroke pipettes. The function of the dispensing tools is described in the
chapter (see Dispensing liquids on p. 29).
Single-channel dispensing tools and eight-channel dispensing tools, with 3 volume ranges each, are
available for the epMotion.
Tab. 5-1: Volume range of the dispensing tools
Single-channel dispensing tool Multi-channel dispensing tool Volume range
TS 50 TM 50 1 µL – 50 µL
TS 300 TM 300 20 µL – 300 µL
TS 10000 TM 1000 40 µL – 1000 µL
The dispensing tool errors can be found in the technical data (see p. 87).
Help on dispensing errors can be found in the "Maintenance" chapter (see Dispensing errors on p. 81)
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5070
6 Dispensing liquids
upper end position
basic position
end of blow-out
AB C
6.1 Basic procedure for dispensing
6.1.1 Operating principle of dispensing tools
Abb. 6-1: Piston-stroke pipette principle
Fig. 6-1: Piston-stroke pipette principle
Dispensing liquids
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29
AAspirate
The piston moves upward from the home position and
aspirates liquid.
BDispense
Liquid dispensing.
CBlow-out
Dispenses the residual liquid
Blow-out in the Pipette dispensing mode
Remaining stroke in the Multidispense
dispensing mode
Multidispense: Piston travels to the home position in
separate steps.
Pipette: Piston travels to the home position in one
step.
The dispensing tools are piston-stroke pipettes that function according to the air cushion principle.
If the piston moves upward in the dispensing tool, liquid will be aspirated into the pipette tip. If the piston
moves downward in the dispensing tool, liquid will be dispensed from the pipette tip. The pistons will be
moved by a motor in the tool holder.
On eight-channel dispensing tools, all 8 channels move simultaneously.
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1 mm
0,7 mm
Dispensing liquids
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6.1.2 Liquid aspiration up to the remaining volume
Abb. 6-2: Liquid aspiration up to the remaining volume with a constant immersion depth
Fig. 6-2: Liquid aspiration up to the remaining volume with a constant immersion depth
The pipette tip is immersed 3 mm into the liquid before liquid aspiration. The pipette tip moves downward
during liquid aspiration. The immersion depth of 3 mm remains constant while the liquid level decreases.
If the pipette tip reaches a distance of 1 mm to the base of the vessel, the pipette tip reduces its immersion
depth to 0.7 mm. If the liquid level continues to sink, the pipette tip will not aspirate any additional liquid.
The remaining volume remains in the vessel.
A remaining volume is present because the pipette tip has a minimum immersion depth in the liquid and a
minimum distance to the base of the vessel. Under normal conditions, the remaining volume is calculated
using a filling level of 1.7 mm.
The distance of the pipette tip to the base of the vessel can be changed in the software (refer to epMotion
software operating manual).
If the liquid is aspirated from the remaining volume, the bend of the liquid level can lead to
dispensing errors.
6.1.3 Bottom tolerance
The bottom tolerance is the distance between the vessel base and the lower end of the pipette tip. The
default setting for the bottom tolerance is 1 mm. For 30 mL and 100 mL reservoirs, the default setting for
the bottom tolerance is 2.5 mm.
Decreasing the bottom tolerance also reduces the remaining volume.
1. Removing liquid above a pellet increases the bottom tolerance.
2. Do not decrease the bottom tolerance on uneven plates.
3. Check the set bottom tolerance when exchanging pipette tips, plates or vessels.
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