Eppendorf 48S User Manual

Guide to Operations

Galaxy S Series

48 S CO

MANUAL No: CO48S-0050

Incubator

2

Revision A

February 16, 2010

New Brunswick Scientific PO Box 4005 44 Talmadge Rd. Edison, 08818-4005 USA

1.800.631.5417 1.732.287.1200 [e-mail]

www.nbsc.com

2

INTERNATIONAL OFFICES:

BELGIUM

New Brunswick Scientific NV-SA
Stationsstraat 180/4
3110 Rotselaar
België/Belgique
Tel: [phone]
Fax: [phone]
E-mail: [e-mail]

CHINA

New Brunswick Scientific
A903 Yin Hai Building
No. 250, Cao Xi Road
Shanghai 200235, P.R. China
Tel: [phone] or 5966
Fax: [phone]
E-mail: [e-mail]

FRANCE

Eppendorf France SARL
60, route de Sartrouville
78230 Le Pecq
France
Tel: [phone]
Fax: [phone]
E-mail: [e-mail]

GERMANY

Eppendorf Vertrieb Deutschland GmbH
New Brunswick Produkte
Peter-Henlein-Strasse 2
D-50389 Wesseling-Berzdorf
Deutschland
Tel: [phone]
Fax: [phone]
E-mail: [e-mail]

THE NETHERLANDS

New Brunswick Scientific BV
Kerkenbos 1101, 6546 BC Nijmegen
Nederland
Tel: [phone]
Fax: [phone]
E-mail: [e-mail]

UNITED KINGDOM

New Brunswick Scientific (UK) Ltd.
17 Alban Park, Hatfield Road
St. Albans, Herts. AL4 0JJ
United Kingdom
Tel: [phone]
Fax: [phone]
E-mail: [e-mail]

CO48S-0050  Galaxy S Series 48 S Personal Size CO2 Incubator User’s Guide

3

CAUTION!

This equipment must be operated as described in this manual.
If operational guidelines are not followed, equipment damage
and personal injury can occur.

Please read the entire User’s Guide before attempting to use this
incubator.

Do not use this equipment in a hazardous atmosphere or with
hazardous materials for which the equipment was not designed.

New Brunswick Scientific (NBS) is not responsible for any
damage to this equipment that may result from the use of an
accessory not manufactured by NBS.

New Brunswick Scientific User’s Guide

4

,

Copyright Notice

New Brunswick Scientific
Box 4005 44 Talmadge Road
Edison, New Jersey 08818-4005

© Copyright 2010 New Brunswick Scientific
All Rights Reserved.

Reproduction, adaptation, or translation without prior written permission from New
Brunswick Scientific is prohibited.

Disclaimer Notice

New Brunswick Scientific reserves the right to change information in this
document without notice. Updates to information in this document reflect our
commitment to continuing product development and improvement.

Manual Conventions

NOTE:

CAUTION!

WARNING!

WARNING!

CRUSH

WARNING!

Notes contain essential information that deserves

special attention.
Caution messages appear before procedures which, if

caution is not observed, could result in damage to the
equipment.

Warning messages alert you to specific procedures or
practices which, if not followed correctly, could result
in serious personal injury.

This particular Warning message represents a potential
electrical hazard.

This particular Warning message, whether found in the
manual or on the incubator means HOT SURFACE–and
therefore represents a potential danger to touch.

Crush Warning messages alert you to specific
procedures or practices regarding heavy objects
not followed correctly, could result in serious personal
injury.

This symbol on the incubator is a reminder that it is of
essential importance to read the user manual.

which

CO48S-0050  Galaxy S Series 48 S Personal Size CO2 Incubator User’s Guide

5

W A R R A N T Y

Every Instrument manufactured by

New Brunswick Scientific (NBS) is warranted to be free

from defects in material an d workmanship. This ap paratus, with

the exception of glassware, lamps and electrodes (where sup plied ),

is warranted for 2 years against faulty components & assembly and

our obligation under this warranty is limited to repairing or replacing

the instrument or part thereof which shall within 2 years following

date of shipment prove to be defective after our exam in ation.

Incubator accessories are warranted for 1 year. This warranty does

not extend to any NBS products which have been subjected

to misuse, neglect, accident or improper installation or

application; nor shall it extend to products which

have been repaired or altered outside the NBS

factory without prior authorization from

New Brunswick Scientific.

New Brunswick Scientific User’s Guide

6

CO48S-0050  Galaxy S Series 48 S Personal Size CO2 Incubator User’s Guide

7

TABLE OF CONTENTS

1 INTRODUCTION..............................................................................................................9

2 UNPACKING & INSTALLATION ...............................................................................11

2.1 INSPECTION OF BOXES.................................................................................................11

2.2 UNPACKING................................................................................................................. 11

2.3 UTILITIES ....................................................................................................................12

2.4 LOCATION ...................................................................................................................13

2.5 INSTALLING THE FEET .................................................................................................13

2.6 SETTING UP................................................................................................................. 14

3 OPERATION....................................................................................................................17

3.1 CONTROL PANEL .........................................................................................................17

3.2 PREPARING FOR OPERATION........................................................................................18

3.3 USING THE HUMIDITY TRAY & HUMIDITY CONTROL..................................................18

3.4 SETTING TEMPERATURE & CO2..................................................................................19

3.5 REFERENCING CO

3.6 CO

RECOVERY...........................................................................................................21

2

3.7 PROGRAMMING THE ALARM SYSTEM..........................................................................22

3.7.1 Setting High & Low Temperature Alarms.......................................................... 22

3.7.2 Setting the CO2 High & Low Alarms.................................................................. 22

3.7.3 Door Open Alarm............................................................................................... 22

3.7.4 Alarm Duration ..................................................................................................23

3.7.5 Alarm Arming Delay...........................................................................................23

3.8 CHAMBER ALARM SYSTEM .........................................................................................23

3.8.1 Temperature Sensor System Alarms...................................................................25

3.8.2 Over-Temperature Cut-out & Alarm.................................................................. 26

3.8.3 CO2 Control System Alarm................................................................................. 26

3.8.4 Review of Alarm Messages.................................................................................27

WITH AUTOZERO ..........................................................................20

2

4 ROUTINE MAINTENANCE..........................................................................................28

4.1 GENERAL NOTES.........................................................................................................28

4.2 D

AILY CHECKS............................................................................................................28

4.3 MONTHLY CHECK .......................................................................................................28

5 CLEANING & DISINFECTING.................................................................................... 30

5.1 CLEANING ...................................................................................................................30

5.2 D

ISINFECTING.............................................................................................................. 30

6 SPECIFICATIONS..........................................................................................................33

7 OPTIONS & ACCESSORIES.........................................................................................35

7.1 OPTIONS ......................................................................................................................35

7.2 AVAILABLE ACCESSORIES........................................................................................... 35

New Brunswick Scientific User’s Guide

8

8 DRAWINGS & TABLES.................................................................................................36

8.1 L

8.2 L

IST OF DRAWINGS...................................................................................................... 36

IST OF TABLES...........................................................................................................36

9 CE CERTIFICATION..................................................................................................... 37

10 INDEX..............................................................................................................................38

CO48S-0050  Galaxy S Series 48 S Personal Size CO2 Incubator User’s Guide

9

1

1

The Galaxy 48 S CO2 incubator is microprocessor controlled and designed to ensure accurate
and reliable operation. The incubator incorporates a simple, door-mounted touch-sensitive
keypad with two individual three-digit LED displays that allow for easy programming and
monitoring of the chamber conditions.

A direct heating system, utilizing a thermal heating element, completely surrounds the
incubator, providing an even temperature within the seamless chamber. The independently and
directly heated outer door is designed to ensure an even distribution of heat. This system
ensures a rapid, controlled return to optimum chamber conditions after a door opening while
also preventing any overshoot. The incubator’s direct heat system provides for optimal use of
laboratory space by allowing the most efficient internal volume for the footprint of the
instrument. A viewing window within the door allows for sample viewing without disturbing
chamber conditions.

A solid-state infrared sensor is used to control the level of CO2, providing excellent reliability
and remaining unaffected by humidity. The CO2 system has a programmable automatic zero
system (AutoZero) to re-reference the sensor baseline to atmospheric CO2 levels at regular
intervals. A small pump supplies HEPA-filtered atmospheric gas to the sensor. The chamber
atmosphere within the sensor is completely displaced, allowing the control system to
automatically reference the sensor, after which the pump is switched off, allowing the chamber
atmosphere to homogenize back into the sensor. This provides for accurate CO2 control
without disturbing the chamber environment.

An independently controlled water tray at the bottom of the incubator allows a high, uniform
relative humidity while preventing condensation in other parts of the chamber. Perforated
shelves are provided as standard to facilitate recovery of RH conditions in the chamber.

The seamless 48-liter chamber and all internal components are manufactured from polished
stainless steel. The shelves (which are non-tip), shelf racks and humidity tray are easily
removed without tools for thorough cleaning and are capable of being sterilized. Air
circulation is achieved without the use of a fan, eliminating ductwork (a potential source of
contamination), simplifying cleaning, eliminating vibration, and reducing small sample
evaporation within the chamber. The outer shell of the incubator is manufactured from
powder-painted steel for a durable finish.

I

NNTTRROODDUUCCTTIIOON

I

N

New Brunswick Scientific User’s Guide

10

The Galaxy 48 S model contains many standard features usually seen as options. In addition to
its viewing window to allow visualization of the cultures without compromising the internal
atmosphere, there is a 25mm access port now standard to allow for seamless integration of
independent probes or other equipment through the chamber. NBS has also included an RS232
port as standard on all Galaxy S series incubators. This port will communicate with any
computer through a hyperlink access or can be used to externally datalog the incubator through
NBS software.

The incubator incorporates a two-level alarm system. The chamber-monitoring alarms are
programmable and will alert you if temperature or CO2 have not recovered within a preset time
after the door has been opened. If it is not required, this system can be disarmed. The system
alarms occur only if a system component problem has developed that requires user intervention
to rectify. The incubator also incorporates an over-temperature safety system that operates
independently from the main control system.

CO48S-0050  Galaxy S Series 48 S Personal Size CO2 Incubator User’s Guide

11

2

2

2.1 Inspection of Boxes

After you have received your order from New Brunswick Scientific, inspect the boxes
carefully for any damage that may have occurred during shipping. Report any damage
to the carrier and to your local NBS Sales Order Department or distributor immediately.

2.2 Unpacking

CRUSH WARNING!

At least two people are required to safely lift your 48 S.

Disassemble the wooden shipping crate and remove the protective packing. Save the
packing materials for possible future use, and be sure to save this User’s Guide for
instruction and reference.

U

NNPPAACCKKIINNGG

U

&

&

I

NNSSTTAALLLLAATTIIOON

I

N

CAUTION!

NEVER try to lift the incubator by its door; this would cause permanent
damage to the incubator.
NEVER lean on or place objects on the open door.

To simplify lifting the incubator, leave it on the pallet. You must have at least one
person at either side to safely lift the incubator. Supported by the base only, remove the
incubator by lifting it from the delivery pallet.

Locate and remove the parts stored in the Humidity Tray.

If any part of your order was damaged during shipping, is missing, or fails to operate,
please fill out Customer Satisfaction Form 6300 (packed in the envelope with your
warranty card) and return it by fax or mail. You can also call New Brunswick
Scientific’s or your distributor’s service department.

Using your NBS packing list, verify that you have received the correct materials and
that nothing is missing.

Table 1 outlines the accessory items that are supplied with your new incubator:

New Brunswick Scientific User’s Guide

12

Table 1: Accessories Provided

Quantity Item Notes

3 Non-tip Shelves Packed Separately
2 Wire Shelf Racks Packed Separately
1 Humidity Tray Packed Separately

14 Silicone Rubber Suction Feet Installed

1 White porous CO2 Sensor Cover Installed
1 Black Sensor Cover* Installed
1 Power Cord Packed in accessories bag

3 meters,

9.8 feet
2 Hose Clips Packed in accessories bag
1 AutoZero HEPA Filter Packed in accessories bag
4 Adjustable Feet Packed in accessories bag
4 Anti-slip Pads for adjustable feet Packed in accessories bag
1 User Manual Provided

* There is a holder at the rear of the incubator to store the black sensor cover.

PVC Tubing, ~1/4-inch or 6mm bore, with an
inline CO

HEPA-filter connected, ready for use

2

Packed in accessories bag

WARNING!

Anytime you touch or handle the white CO2 sensor cover, be sure to
wear gloves, and do not later touch those gloves to your face. Discard
or wash the gloves.

2.3 Utilities

In order to use the incubator, you will need:

Table 2: Utilities

Utility Requirement

Electricity

CO2 Gas Cylinder with 100% CO2 vapor withdrawal, together with a two-stage regulator

100/120V, 50/60 Hz grounded electrical supply with minimum capacity of 6
amps (or 8 amps for High Temperature Disinfection Models)

OR
220/240V, 50 Hz grounded electrical supply with minimum capacity of 3 amps
(or 5 amps for High Temperature Disinfection Models)

for pressure control to 5 psi or 0.35 bar

WARNING!

For proper incubator operation, CO2 gas pressure must not exceed 5 psi.

CO48S-0050  Galaxy S Series 48 S Personal Size CO2 Incubator User’s Guide