Eppendorf 48R User Manual
Guide to Operations
Galaxy R Series
48 R CO
MANUAL No: CO48R-0050
Incubator
2
Revision B
February 16, 2010
New Brunswick Scientific PO Box 4005 44 Talmadge Rd. Edison, 08818-4005 USA
1.800.631.5417 1.732.287.1200 bioinfo@nbsc.com
www.nbsc.com
2
INTERNATIONAL OFFICES:
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Tel: +32 (0)16 56 28 31
Fax: +32 (0)16 57 27 53
E-mail: sales@nbsnv-sa.be
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Tel: +86 21 6484 5955 or 5966
Fax: +86 21 6484 5933
E-mail: nbschc@online.sh.cn
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78230 Le Pecq
France
Tel: +33 (0)1 30 15 67 40
Fax: +33 (0)1 30 15 67 45
E-mail: eppendorf@eppendorf.fr
GERMANY
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New Brunswick Produkte
Peter-Henlein-Strasse 2
D-50389 Wesseling-Berzdorf
Deutschland
Tel: +49 (0)2232 418 0
Fax: +49 (0)2232 418 155
E-mail: vertrieb@eppendorf.de
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Nederland
Tel: +31 (0)24 3717 600
Fax: +31 (0)24 3717 640
E-mail: sales@nbsbv.nl
UNITED KINGDOM
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St. Albans, Herts. AL4 0JJ
United Kingdom
Tel: +44 (0)1727 853 855
Fax: +44 (0)1727 835 666
E-mail: bioinfo@nbsuk.co.uk
CO48R-0050 Galaxy R Series 48 R Personal Size CO2 Incubator User’s Guide
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CAUTION!
This equipment must be operated as described in this manual.
If operational guidelines are not followed, equipment damage
and personal injury can occur.
Please read the entire User’s Guide before attempting to use this
incubator.
Do not use this equipment in a hazardous atmosphere or with
hazardous materials for which the equipment was not designed.
New Brunswick Scientific (NBS) is not responsible for any
damage to this equipment that may result from the use of an
accessory not manufactured by NBS.
New Brunswick Scientific User’s Guide
4
,
Copyright Notice
New Brunswick Scientific
Box 4005 44 Talmadge Road
Edison, New Jersey 08818-4005
© Copyright 2010 New Brunswick Scientific
All Rights Reserved.
Reproduction, adaptation, or translation without prior written permission from New
Brunswick Scientific is prohibited.
Disclaimer Notice
New Brunswick Scientific reserves the right to change information in this
document without notice. Updates to information in this document reflect our
commitment to continuing product development and improvement.
Manual Conventions
NOTE:
CAUTION!
WARNING!
WARNING!
CRUSH
WARNING!
Notes contain essential information that deserves
special attention.
Caution messages appear before procedures which, if
caution is not observed, could result in damage to the
equipment.
Warning messages alert you to specific procedures or
practices which, if not followed correctly, could result
in serious personal injury.
This particular Warning message represents a potential
electrical hazard.
This particular Warning message, whether found in the
manual or on the incubator means HOT SURFACE–and
therefore represents a potential danger to touch.
Crush Warning messages alert you to specific
procedures or practices regarding heavy objects
not followed correctly, could result in serious personal
injury .
This symbol on the incubator is a reminder that it is of
essential importance to read the user manual.
which
CO48R-0050 Galaxy R Series 48 R Personal Size CO2 Incubator User’s Guide
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W A R R A N T Y
Every Instrument manufactured by
New Brunswick Scientific (NBS) is warranted to be free
from defects in material and workmanship. This apparatus, with
the exception of glassware, lamps and electrodes (where supplied),
is warranted for 2 years against faulty components & assembly and
our obligation under this warranty is limited to repairing or replacing
the instrument or part thereof which shall within 2 years following
date of shipment prove to be defective after our examination.
Incubator accessories are warranted for 1 year. This warranty does
not extend to any NBS products which have been subjected
to misuse, neglect, accident or improper installation or
application; nor shall it extend to products which
have been repaired or altered outside the NBS
factory without prior authorization from the
New Brunswick Scientific.
New Brunswick Scientific User’s Guide
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CO48R-0050 Galaxy R Series 48 R Personal Size CO2 Incubator User’s Guide
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TABLE OF CONTENTS
1 INTRODUCTION............................................................................................................11
2 UNPACKING & INSTALLATION ...............................................................................13
2.1 INSPECTION OF BOXES.................................................................................................13
2.2 UNPACKING................................................................................................................. 13
2.3 UTILITIES ....................................................................................................................14
2.4 LOCATION ...................................................................................................................14
2.5 INSTALLING THE FEET .................................................................................................15
2.6 SETTING UP................................................................................................................. 15
3 OPERATION....................................................................................................................19
3.1 CONTROL PANEL .........................................................................................................19
3.2 PREPARING FOR OPERATION........................................................................................19
3.3 USING THE HUMIDITY TRAY & HUMIDITY CONTROL..................................................20
3.4 PROGRAMMING............................................................................................................21
3.4.1 Temperature and CO2 Level...............................................................................21
3.4.2 User Access Code............................................................................................... 22
3.5 REFERENCING CO
3.6 USER SETTINGS.......................................................................................................... 23
3.6.1 DATE/TIME ADJUST ........................................................................................24
3.6.2 AUDIO VOLUME ADJUST ............................................................................... 24
3.6.3 PROGRAMMABLE CO2 AUTOZERO...............................................................24
3.6.4 DATALOGGER ..................................................................................................25
3.6.5 POWER FREQUENCY ......................................................................................25
3.6.6 DISABLE ............................................................................................................ 25
3.6.7 DISINFECTION (Model Option)....................................................................... 26
3.7 DATALOGGER
3.7.1 ALARM EVENTS................................................................................................26
3.7.2 TEMPERATURE GRAPH + DOOR OPEN BAR CHART.................................27
3.7.3 CO2 GRAPH + DOOR OPEN BAR CHART...................................................... 28
3.7.4 DIAGNOSTIC CHAMBER ELEMENT GRAPH ................................................ 28
3.7.5 DIAGNOSTIC DOOR GRAPH........................................................................... 28
3.7.6 DIAGNOSTIC DOOR ELEMENT GRAPH........................................................29
3.7.7 RESTART GRAPHIC RECORD.........................................................................29
3.8 CHAMBER
ALARMS MENU SCREEN....................................................................... 29
3.8.1 Chamber Alarm System Function....................................................................... 30
3.9 D
IAGNOSTIC MENU SCREEN........................................................................................ 32
3.10 HELP MENU SCREEN ................................................................................................33
SENSOR WITH AUTOZERO............................................................. 23
2
SCREEN ...........................................................................................26
4 ROUTINE MAINTENANCE..........................................................................................34
4.1 G
4.2 D
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ENERAL NOTES.........................................................................................................34
AILY CHECKS............................................................................................................34
8
4.3 MONTHLY CHECK .......................................................................................................34
5 CLEANING & DISINFECTING.................................................................................... 36
5.1 C
5.2 D
LEANING ...................................................................................................................36
ISINFECTING.............................................................................................................. 36
5.3 HIGH TEMPERATURE DISINFECTION............................................................................ 38
6 SPECIFICATIONS..........................................................................................................39
7 OPTIONS & ACCESSORIES.........................................................................................41
7.1 O
7.2 A
PTIONS ......................................................................................................................41
VAILABLE ACCESSORIES........................................................................................... 41
8 APPENDIX A: OPTIONS..............................................................................................42
8.1 O
8.2 H
PTIONS ......................................................................................................................42
IGH TEMPERATURE DISINFECTION............................................................................42
8.3 HIGH TEMPERATURE DISINFECTION OPTION WITH OXYGEN CONTROL....................... 45
8.3.1 Oxygen Sensor Removal..................................................................................... 45
8.3.2 Oxygen Sensor Replacement .............................................................................. 45
8.4 BMS RELAY CONTACT ALARM OPTION...................................................................... 46
8.5 O
CONTROL (1-19%) .................................................................................................47
2
8.5.1 Setting Up the N2 Tank.......................................................................................47
8.5.2 Setting Up Oxygen Control ................................................................................48
8.5.3 Operating Guidelines ......................................................................................... 50
8.5.4 Referencing to Atmosphere ................................................................................50
8.5.5 Replace Sensor Soon .......................................................................................... 51
8.5.6 Replace Sensor Now........................................................................................... 51
8.5.7 Removing & Replacing O2 Sensor......................................................................52
8.5.8 Replacing the Filter Disc ...................................................................................53
8.5.9 Troubleshooting the Oxygen Sensor...................................................................55
8.5.10 Oxygen Control Specifications........................................................................... 55
8.6 O
8.6.1 Setting Up the N
8.6.2 Setting Up O
CONTROL (0.1-19%) ..............................................................................................56
2
Tank.......................................................................................56
2
Control ........................................................................................56
2
8.6.3 Enabling or Disabling O2 Control .....................................................................57
8.6.4 Alarms.................................................................................................................58
8.6.5 Referencing to Atmosphere ................................................................................58
8.6.6 Programming Desired O2 Level.........................................................................58
8.6.7 Precautions......................................................................................................... 59
8.7 O
CONTROL (1-95%) .................................................................................................59
2
8.7.1 Setting Up the N2 Tank.......................................................................................60
8.7.2 Setting Up O2 Control ........................................................................................60
8.7.3 Enabling or Disabling O
8.7.4 Important Notes for O
Control .....................................................................61
2
Levels >80%.................................................................62
2
8.7.5 Alarms.................................................................................................................62
8.7.6 Referencing to Atmosphere ................................................................................63
8.7.7 Programming Desired O
Level.........................................................................63
2
CO48R-0050 Galaxy R Series 48 R Personal Size CO2 Incubator User’s Guide
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8.7.8 Other Precautions ..............................................................................................63
8.7.9 Specifications......................................................................................................63
8.8 O2 SENSOR REPLACEMENT ..........................................................................................63
9 CE CERTIFICATION..................................................................................................... 64
10 DRAWINGS & TABLES................................................................................................65
10.1 INDEX OF DRAWINGS................................................................................................... 65
10.2 INDEX OF TABLES........................................................................................................65
11 INDEX..............................................................................................................................66
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CO48R-0050 Galaxy R Series 48 R Personal Size CO2 Incubator User’s Guide
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The Galaxy 48 R CO2 incubator is microprocessor controlled and designed to ensure accurate
and reliable operation. The incubator incorporates a large, back-lit LCD display, touchsensitive keypad and sophisticated control system that allows for easy programming, control
and monitoring of the chamber conditions. The display also provides for graphical viewing of
system data, on-screen messaging and help files to simplify operation and maintenance.
A direct heating system, utilizing a thermal heating element, completely surrounds the
incubator, providing an even temperature within the seamless chamber. The independently and
directly heated outer door is designed to ensure an even distribution of heat, thereby
eliminating condensation on the inner door. This system ensures a rapid, controlled return to
optimum chamber conditions after a door opening while also preventing any overshoot. The
incubator’s direct heat system provides for optimal use of laboratory space by allowing the
most efficient internal volume for the footprint of the instrument. A viewing window within
the door allows for sample viewing without disturbing chamber conditions.
A solid-state infrared sensor is used to control the level of CO2, providing excellent reliability
and remaining unaffected by humidity. The CO2 system has a programmable automatic zero
system (AutoZero) to re-reference the sensor baseline to atmospheric CO2 levels at regular
intervals. A small pump supplies HEPA-filtered atmospheric gas to the sensor. The chamber
atmosphere within the sensor is completely displaced, allowing the control system to
automatically reference the sensor, after which the pump is switched off, allowing the chamber
atmosphere to homogenize back into the sensor. This provides for accurate CO2 control
without disturbing the chamber environment.
An independently controlled water tray at the bottom of the incubator allows a high, uniform
relative humidity while preventing condensation in other parts of the chamber. Perforated
shelves are provided as standard to facilitate a much faster recovery of RH conditions in the
chamber than with unperforated shelves.
The seamless 48-liter chamber and all internal components are manufactured from polished
stainless steel. The shelves (which are non-tip), shelf racks and humidity tray are easily
removed without tools for thorough cleaning and are capable of being sterilized. Air
circulation is achieved without the use of a fan, eliminating ductwork (a potential source of
contamination), simplifying cleaning, eliminating vibration, and reducing small sample
evaporation within the chamber. The outer shell of the incubator is manufactured from zincplated stove enamelled steel to give a durable corrosion-resistant finish.
I
NNTTRROODDUUCCTTIIOON
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The Galaxy 48 R model contains many standard features usually seen as options. It has a
sealing inner door with a cam action lock to allow visualization of the cultures without
compromising the internal atmosphere. This is also available as a split 4- or 8-inner-door
option (to coordinate with shelves), which is ideal for critical hypoxic studies. In addition,
there is a 25mm access port now standard to allow for seamless integration of independent
probes or other equipment through the chamber. Lastly, NBS has included an RS232 port as
standard on all Galaxy R series incubators. This port will communicate with any computer
through a hyperlink access or can be used to externally datalog the incubator through NBS
software.
The incubator incorporates a two-level alarm system. The chamber-monitoring alarms are
programmable and will alert you if temperature or CO2 have not recovered within a preset time
after the door has been opened. If it is not required, this system can be disarmed. The system
alarms occur only if a system component problem has developed that requires user intervention
to rectify. The incubator also incorporates an over-temperature safety system that operates
independently from the main control system.
The incubator features multiple options that can be installed to simplify maintenance and
provide superior control over experimental conditions. For example, high-temperature
disinfection quickly and conveniently disinfects the incubator’s chamber at 120oC, without the
need to remove interior components or the CO2 sensor. A humidity tray warning system
warns the user before the humidity tray runs out of water, preventing dehydration of samples.
Oxygen control provides for conditions that require above- or below-ambient oxygen levels.
These and other options and accessories provide for a uniquely flexible CO2 incubator capable
of meeting the most demanding requirements.
CO48R-0050 Galaxy R Series 48 R Personal Size CO2 Incubator User’s Guide
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22
2.1 Inspection of Boxes
After you have received your order from New Brunswick Scientific, inspect the boxes
carefully for any damage that may have occurred during shipping. Report any damage
to the carrier and to your local NBS Sales Order Department or distributor immediately.
2.2 Unpacking
CRUSH WARNING!
At least two people are required to safely lift your 48 R.
Disassemble the wooden shipping crate and remove the protective packing. Save the
packing materials for possible future use, and be sure to save this User’s Guide for
instruction and reference.
To simplify lifting the incubator, leave it on the pallet. You must have at least one
person at either side to safely lift the incubator. Supported by the base only, remove the
incubator by lifting it from the delivery pallet.
Locate and remove the parts stored in the Humidity Tray.
If any part of your order was damaged during shipping, is missing, or fails to operate,
please fill out Customer Satisfaction Form 6300 (packed in the envelope with your
warranty card) and return it by fax or mail. You can also call New Brunswick
Scientific’s or your distributor’s service department.
Using your NBS packing list, verify that you have received the correct materials and
that nothing is missing.
Table 1 on the following page outlines the accessory items that are supplied with your
new incubator:
U
NNPPAACCKKIINNGG
U
&
&
I
NNSSTTAALLLLAATTIIOON
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Table 1: Accessories Provided
Quantity Item Notes
3 Non-tip Shelves Packed Separately
2 Wire Shelf Racks Packed Separately
1 Humidity Tray Packed Separately
14 Silicone Rubber Suction Feet Installed
1 White porous CO2 Sensor Cover Installed
1 Black Sensor Cover* Installed
1 Power Cord Packed in accessories bag
3 meters,
9.8 feet
2 Hose Clips Packed in accessories bag
1 AutoZero HEPA Filter Packed in accessories bag
4 Adjustable Feet Packed in accessories bag
4 Anti-slip Pads for adjustable feet Packed in accessories bag
1 User Manual Provided
PVC Tubing, ~1/4-inch or 6mm bore,
with an inline CO
HEPA-filter
2
Packed in accessories bag
connected, ready for use
* There is a holder at the rear of the incubator to store the black sensor cover.
WARNING!
Anytime you touch or handle the white CO2 sensor cover, be sure to
wear gloves, and do not later touch those gloves to your face. Discard
or wash the gloves.
2.3 Utilities
In order to use the incubator, you will need:
Table 2: Utilities
Utility Requirement
Electricity
CO2 Gas Cylinder with 100% CO2 vapor withdrawal, together with a two-stage regulator
2.4 Location
The incubator is designed to operate at a chamber temperature of 4.0°C above ambient,
and at an absolute minimum ambient temperature of 15°C if the incubator is being used
at 37°C. Care should be taken to avoid placing the incubator in a position that may
affect its performance, such as those listed below.
100/120V, 50/60 Hz grounded electrical supply with minimum capacity of 6
amps (or 8 amps for High Temperature Disinfection Models)
OR
220/240V, 50 Hz grounded electrical supply with minimum capacity of 3 amps
(or 5 amps for High Temperature Disinfection Models)
for pressure control to 5 psi or 0.35 bar
CO48R-0050 Galaxy R Series 48 R Personal Size CO2 Incubator User’s Guide
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DO NOT place the incubator:
• Directly under, beside or within the air-flow of heating or air-conditioning
ducts, or other drafts;
• Directly beside heat-generating equipment such as a heater, an autoclave or
an oven;
• Near the exhaust of heat- or cold-generating equipment (like a –86oC
freezer);
• Near a window exposed to direct, unshaded sunlight.
Place the incubator in the working position, on a level surface capable of bearing its
weight of approximately 70.5 lbs/32 kg (actual use weight will be heavier and will
depend on the options installed and the material stored in the incubator).
The incubator is not designed to be directly stackable. A second incubator may be
safely stacked on top of another identical incubator by using the custom-designed
stacking stand available as an accessory for the Galaxy 48 R. It is not possible to put
any other type of incubator or heavy apparatus on top, as the top cover and stacking
stand were not designed to support any other device.
2.5 Installing the Feet
To ensure adequate airflow for correct operation of the relative humidity control system
the incubator feet must be installed. More than one person is required to perform this
operation.
To insert the adjustable feet:
1. If they are not already installed, install the locking nuts onto each of the four feet
provided.
2. Beginning with the front pair: tilt the incubator toward the back, and screw the feet
in, to the required depth.
3. Tilt the incubator forward to install the rear pair of feet.
4. Put an anti-slip pad (provided) on each foot.
2.6 Setting Up
Install the power cord:
1. Insert the power cord into its receptacle on the back on the incubator.
2. Press the cord firmly into its socket.
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Install the shelf racks and shelves, and level the incubator:
1. Each wire shelf rack has silicone suction cups that hold the rack in place. Install the
silicone suction cups onto the wire rack supports (7 per rack, each marked with an
asterisk in Figure 1a below).
2. Note that there are lefthand and righthand racks. The suction cup marked R
(“Rear”) goes to the rear of the chamber as shown in Figure 1b below. The suction
cups will adhere to the chamber walls even if they are dry; but if you feel it is
necessary, you can dampen them with distilled water to increase adhesion.
3. Ensure the shelf racks are installed squarely in the chamber so the shelves will sit
on a level plane (see Figure 1c below).
Figure 1: Installing Shelf Racks
R
a b c
4. Install the three shelves, making sure that each shelf’s anti-tip tab is properly
inserted onto each of the wire shelf rack guides (see Figure 2 below).
Figure 2: Installing Shelves
1
1 Anti-tip tab
CO48R-0050 Galaxy R Series 48 R Personal Size CO2 Incubator User’s Guide
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5. Level the incubator by adjusting the feet. Place a small level on the second shelf of
the incubator. Adjust the leveling feet until the incubator is level and stable. Lock
the leveling legs in place by tightening the locking nuts on each leg.
Install the humidity tray:
1. Install the humidity tray in its location beneath the lowest shelf rack position: insert
the tray diagonally (see Figure 3a), then turn the tray 45° as shown, until you can
set it in position, resting on the chamber floor, with its front and rear edges resting
just above the shelf rack supports (see Figure 3b).
Figure 3: Installing/Removing the Humidity Tray
1
a b
1 Center the tray, making sure it sits within the rack supports.
Connect the CO2 gas supply:
1. Connect the incubator to the CO2 supply using the ~1/4-inch or 6mm plastic tubing
(with installed HEPA filter) by attaching the tubing from the two-stage regulator (or
in-line regulator) to the matching CO2 inlet on the rear of the incubator.
NOTE:
It is highly recommended that an in-line regulator be used at the
incubator’s gas inlet(s).
2. Use the tubing clips provided to eliminate CO
leaks.
2
WARNING!
Slightly increased levels of CO2 may be found in and around the operating
area of the CO2 incubator. Over time, this can have adverse effects on
those exposed to such an environment. Users working in environments
with elevated levels of CO2 should take all appropriate precautions to
protect their breathing.
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Install the HEPA AutoZero filter:
1. Press the AutoZero HEPA filter gently into the white plastic filter socket at the top
of the rear panel of the incubator (see Figure 4).
Figure 4: AutoZero HEPA Filter & Fitting
CO48R-0050 Galaxy R Series 48 R Personal Size CO2 Incubator User’s Guide
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3.1 Control Panel
The control panel consists of an LCD display, five function keys & four direction keys
(see Figure 5):
2
1 Direction keys 2 Function keys
The purpose of each Function Key is identified at the bottom of the display (above each
key); the function may change from screen to screen.
The cluster of four arrowed Direction Keys will move the cursor around the screen and
adjust values.
The HELP file contains most of the information in this User Manual, together with more
detailed troubleshooting information.
33
Figure 5: Control Panel
O
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1
3.2 Preparing for Operation
1. Remove the black protective cover from the CO2 sensor (located on the back
surface of the internal chamber), and store it for use when you clean the incubator.
There is a storage holder for the cover on the back on the incubator.
2. Ensure that the white porous sensor cover remains in place.
3. Using the power cord provided, connect the incubator to a grounded power supply.
4. Switch the incubator ON using the on/off switch at the rear of the cabinet. The
display will illuminate immediately.
5. Turn on the CO2 gas supply with the pressure regulator set to 5 psi or 0.35 bar.
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NOTE:
Be certain to check for leaks in the CO2 connections to avoid depleting
your CO2 gas supply. This can be accomplished using a solution of
soapy water applied to each fitting and checking for bubbles. If any
bubbles are noted, readjust the fitting.
6. The chamber setpoints are pre-programmed at 37.0°C and 5% CO
incubator on until the programmed chamber temperature and CO
. Leave the
2
concentration has
2
been reached (please see NOTE below).
NOTE:
The incubator’s CO2 valve is disabled until the incubator reaches the
temperature setpoint. After reaching the temperature setpoint, the CO
valve is activated, allowing the incubator to reach the CO
setpoint. If
2
power is interrupted to the incubator long enough for the temperature to
drop below setpoint, the CO
valve will be deactivated until setpoint is
2
again achieved. (This serves to avoid spurious CO2 readings while the
incubator is reaching its temperature setpoint)
7. Leave the incubator running for at least two hours (preferably overnight) to allow
conditions to stabilize.
2
3.3 Using the Humidity Tray & Humidity Control
If humidification is required, the humidity tray should be filled with 0.5 liters of warm
(∼ 37.0ºC) distilled water at this time.
For cell culture work, we recommend the use of copper sulphate (or a recognized
biocide) in the humidity tray. Tests have shown that, in addition to inhibiting bacterial
growth in the tray, this can reduce contamination on the chamber walls. Add one small
teaspoonful (~0.11 oz or 3.2 g) of copper sulphate each time you fill the tray with fresh
water.
For IVF and other sensitive work, we do not recommend the use of any biocide in the
humidity tray. To reduce the possibility of contamination, every 10 to 14 days, empty
the tray, clean it with a solution of 70% isopropyl alcohol and 30% distilled water, and
then refill it with 0.5 liters of warm distilled water.
To remove the humidity tray, turn it 45º to clear the shelf rack supports (see Figure 3a).
CO48R-0050 Galaxy R Series 48 R Personal Size CO2 Incubator User’s Guide
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CAUTION!
Never leave water in the humidity tray while the incubator is switched
off, or when a high temperature disinfection cycle is initiated, to avoid
possible damage to the CO2 sensor.
The humidity level within the chamber can be manually adjusted from ~95% to ~97%.
The controller to adjust the humidity level is found on the lower, front right side of the
instrument. The black adjustment knob can be rotated clockwise for standard (~95%)
humidity levels, and counter-clockwise to increase the humidity level.
NOTE:
The humidity tray should always be left in place, even if the incubator is
not being humidified.
3.4 Programming
3.4.1 Temperature and CO2 Level
To set the desired operating temperature and CO2 level:
1. Press the PROG function key (far left key):
2. In the PROG screen that appears, press the desired function key, TEMP or
CO2, then use the W & X direction keys to adjust the value.
NOTE:
If the incubator is supplied with the option of oxygen control, the setpoint
for the oxygen level can be selected and changed like the temperature
and CO2 setpoints.
3. When the desired setpoint is displayed, press the ENTER function key.
4. If you have made adjustments, allow the incubator to stabilize at the
setpoints before continuing. (See important NOTE on the following page.)
New Brunswick Scientific User’s Guide
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