Eppendorf 44R User Manual

Operating manual

Draft

—

Biological Shakers Innova® 44/44R

see

on p.Fig.Tab.p

erating manua

M1282-0050

New Brunswick™ Biological Shakers

Innova® 44/44R

Operating manual

Eppendorf

and the Eppendorf logo are registered trademarks of Eppendorf AG, Germany

New Brunswick™ and the New Brunswick™ logo are trademarks of Eppendorf AG, Germany.

Innova® is a registered trademark of New Brunswick Scientific Co., Inc., USA.

Windows

is a registered trademark of Microsoft Corporation in the United States and other countries.

Trademarks are not marked in all cases with ™ or ® in this manual.

M1282-0050-N/022013



Biological Shakers Innova® 44/44R — Operating manual

Table of contents

1 Table of contents

1 User instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.1 Hazard symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.3 Application limits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.3.1 Description of ATEX Guideline (94/9EC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.5 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.5.1 Personal injury and damage to device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.5.2 Incorrect handling of accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3 Product description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.1 Main illustration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.2 General overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

4 Inspection and unpacking of equipment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.1 Inspection of boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.2 Packing list verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.3 Unpacking of equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.4 Out of box concerns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.5 Warranty registration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Table of contents

5 Preparing the location. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5.1 Physical location. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5.2 Environment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5.3 Electrical requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5.4 Space requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

6 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

6.1 Tools required for installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

6.2 Install the front grille . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

6.3 Level a single shaker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

6.4 Level the optional base. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

6.5 Mount Innova 44/44R on optional base . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

6.6 Install the stacking kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

6.6.1 For two 44/44R shakers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

6.6.2 For an Innova 4400/4430 on an Innova 44/44R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

6.7 Stack two Innova 44/44R shakers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

6.8 Stack a 4400/4430 on a 44/44R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

6.9 Stack a third shaker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

7 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

7.1 Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

7.2 LCD display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Table of contents

Biological Shakers Innova® 44/44R — Operating manual

7.3 Changing screens. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

7.4 Display icons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7.5 Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

7.5.1 Optional remote alarm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

7.6 Glide-up door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.7 Spill pan/water reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.8 Software interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.9 Interior light(s). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.10 Heater. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.11 Refrigeration (44R only) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.12 Service accessibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

8 Getting started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

8.1 Platform assemblies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

8.2 Installation of platform. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

8.3 Install flask clamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

8.4 Electrical connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

8.5 Optional gassing manifold kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.6 Optional UV germicidal lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.7 Optional photosynthetic lamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.8 Optional humidity monitor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.9 Fill the water reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.10 Drain the water reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

9 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

9.1 Safety precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

9.2 Open the door. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

9.3 Start the shaker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

9.4 Using the LCD screens. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

9.4.1 Display screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

9.4.2 Summary screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

9.4.3 Setup screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

9.4.4 Lamps screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

9.4.5 RS232 Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

9.4.6 Calibrate screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

9.4.7 Programs screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

9.5 Program the shaker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

9.5.1 Timer only . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

9.5.2 Programmed steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

9.5.3 Create a program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

9.5.4 Edit a program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

9.5.5 Run a program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

9.6 Mute the audible alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

9.7 Temperature offset calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

9.7.1 Calculate the offset value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

9.7.2 Set the offset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

9.8 Using Calspeed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

9.9 Power interruption. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

9.10 Slide-out platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

10 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

10.1 General troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

11 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

11.1 Routine maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

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Biological Shakers Innova® 44/44R — Operating manual

11.2 Cleaning external and internal surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

11.3 Biohazard decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

12 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

12.1 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

13 Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

13.1 Replacement parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

13.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

13.2.1 Slide-out platforms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

13.2.2 Flask clamps for universal platforms. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

13.2.3 Replacement clamp hardware kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

13.2.4 Test tube racks and other accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

13.2.5 Optional bases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

13.2.6 Optional stacking kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

13.2.7 Optional handles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

14 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

14.1 Transport and storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

14.2 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

15 Appendix A: Remote programming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

15.1 Setting up with HyperTerminal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

15.2 Overview of command sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

15.3 Index to command codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

15.4 Set commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

15.5 Profile control commands. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

15.6 Report request commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

15.7 Set/Get date and time. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

Table of contents

16 Appendix B: Certifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

16.1 Certifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

Biological Shakers Innova® 44/44R — Operating manual

Table of contents

Biological Shakers Innova® 44/44R — Operating manual

1 User instructions

1.1 Using this manual



Carefully read this operating manual before using the device for the first time.

 Also observe the operating manual enclosed with the accessories.

 The operating manual should be considered as part of the product and stored in a location

that is easily accessible.

 When passing the device on to third parties, be sure to include this operating manual.

 If this manual is lost, please request another one. The current version can be found on our

website www.eppendorf.com.

1.2 Danger symbols and danger levels

1.2.1 Hazard symbols

Hazard point Burns

Electric shock Material damage

User instructions

Explosion Heavy loads

Inhalation

1.2.2 Degrees of danger

The following degree levels are used in safety messages throughout this manual. Acquaint yourself with each item and the potential risk if you disregard the safety message.

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.

CAUTION May lead to light to moderate injuries.

NOTICE May lead to material damage.

1.3 Symbols used

Example Meaning

 You are requested to perform an action.

Perform these actions in the sequence described.

• List.

References useful information.

Safety

Biological Shakers Innova® 44/44R — Operating manual

2 Safety

2Safety

2.1 Intended use

This device is exclusively intended for indoor use and for uniform movement and temperature control of biological solutions and cultures in reaction vessels.

Lack of safety due to incorrect accessories or spare parts

Accessories and spare parts that are not recommended by Eppendorf compromise the safety,

CAUTION!

2.2 User profile

2.3 Application limits

2.3.1 Description of ATEX Guideline (94/9EC)

function and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of non-recommended accessories and spare parts.

 Only use accessories and original spare parts recommended by Eppendorf.

The device may only be operated by trained lab personnel who have carefully read this operating manual and are familiar with the device functions.

Explosion hazard

 Do not operate the device in areas where work is completed with explosive substances.

DANGER!

 Do not use this device to process any explosive or highly reactive substances.

 Do not use this device to process any substances which could create an explosive

atmosphere.

Due to its design and the ambient conditions in its interior, the device is not suitable for use in potentially explosive atmospheres.

The device may only be used in a safe environment, e.g., the open atmosphere of a ventilated lab.

The use of substances which may contribute to a potentially explosive atmosphere is not permitted.

The final decision regarding the risks associated with using these types of substances is the user's responsibility.

2.4 Information on product liability

In the following cases, the designated protection of the device may be compromised.

The liability for the function of the device passes to the operator if:

• The device is not used in accordance with this operating manual.

• The device is used outside of the range of application described in the succeding chapters.

• The device is used with accessories or consumables that were not approved by Eppendorf.

• Service or maintenance is completed on the device by people who are not authorized by

Eppendorf.

• The owner has made unauthorized modifications to the device.

2.5 Warnings for intended use

Before using the device, read this operating manual and observe the following general safety instructions.

Biological Shakers Innova® 44/44R — Operating manual

2.5.1 Personal injury and damage to device

Electric shock due to damage to the device or power cable

 Only switch on the device if the device and power cable are undamaged.

WARNING !

 Only use devices that have been properly installed or repaired.

Lethal voltages inside of the device

 Ensure that the housing is always closed and undamaged so the user cannot accidentally

touch the parts inside.

 Do not remove the housing of the device.

Danger due to incorrect power supply

 Only connect the device to voltage sources that meet the requirements on the name plate.

 Only use sockets with a protective earth (PE) conductor and suitable power cable.

Health risk due to contact with infectious liquids and pathogenic bacteria

 Observe the national regulations for handling these substances, the biological security level

of your laboratory, the material safety data sheets and the manufacturer's application notes.

 Wear personal protective equipment (PPE).

 Follow the instructions regarding hygiene, cleaning and decontamination.

 For complete instructions on the handling of germs or biological material in risk group II or

higher, please refer to the "Laboratory Biosafety Manual" (source: World Health Organization).

Safety

WARNING !

CAUTION!

NOTICE!

Health risk due to poisonous, radioactive or aggressive chemicals

 Observe the national regulations for handling these substances as well as the material safety

data sheets and manufacturer's application notes.

 Wear personal protective equipment (PPE).

Burns due to hot metal on the device and hot flasks

 Only touch the device and flasks when wearing protective gloves.

Risk of crushing due to missing auxiliary aid

 Lifting and transporting the shaker without suitable technical aids can result in crushing and

other injuries.

 Use a hydraulic lifting platform to install and uninstall the shaker.

Risk to health due to lifting heavy loads

 Only lift the device with another person or using a suitable aid.

 Make sure to use a transport aid for transportation over long distances.

Damage to electronic components due to condensation

Condensation can form inside the device after the device has been moved from a cool to a warmer environment.

 Wait at least three hours before connecting it to the mains/power supply.

Safety

Biological Shakers Innova® 44/44R — Operating manual

2.5.2 Incorrect handling of accessories

Lack of safety due to incorrect accessories or spare parts

Accessories and spare parts that are not recommended by Eppendorf compromise the safety,

CAUTION!

NOTICE!

function and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of non-recommended accessories and spare parts.

 Only use accessories and original spare parts recommended by Eppendorf.

Danger due to damaged tubes

Small scratches and cracks can cause severe damage to the device and accessories. Liquids may escape.

 Before use, visually check all tubes for any damage.

 Never use damaged tubes.

Biological Shakers Innova® 44/44R — Operating manual

Incuba

or Shaker Series

NEW BRUNSWICK SCIENTIFIC

7321456

11 10 9 8

3 Product description

3.1 Main illustration

Abb. 1: Front and rear view of the In nova 44/44R

Fig. 1: Front and rear view of the Innova 44/44R

1Door

With automatic stop function

2 Door handle

Product description

3 Start/Stop switch

Start or stop drive

5 Push button control knob

Set or change the parameters or Start or stop drive

7 Name plate

Model number, documentation number, serial number and electrical connection data

9 Mains power connection

Connect the power cable

11 Discharge hose

Discharge hose with clamp

4Display

Graphical user interface with display of parameters and parameter values

6 Mains power switch

Switch device on or off

8 Base stand

Align the device horizontally.

10 RS-232 interface

Read out parameter values and control operational functions using computer applications

Biological Shakers Innova® 44/44R — Operating manual

Abb. 2: Internal view

1234

Fig. 2: Internal view

1 Halogen lighting 2 Drive

Eccentric drive - orbit is model-dependent.

3 Drip pan reservoir

Catch liquids and store water

4 Platform frame

Remove the platform and platform frame.

3.2 General overview

The Innova 44/44R stackable incubator and incubator refrigerated shakers are large-capacity

Product description

orbital shakers that utilize a triple eccentric counter-balanced drive mechanism. They provide horizontal plane rotary motion in either a 2.54 cm (1 in) or a 5 cm (2 in) diameter circular orbit, depending on the model. A Proportional/Integral (PI) microprocessor controller with instantaneous digital feedback controls the speed over the entire speed range.

The Innova 44R provides temperature control from 20 °C below ambient (as low as 4 °C) to 80 °C, and the Innova 44 from 5 °C above ambient to 80 °C. Naturally, both these ranges depend on relative humidity and other ambient factors, as well as the options installed in the unit. Ambient temperature is measured at one meter from the exterior of the unit.

Erlenmeyer flasks (up to 5 liters in size) as well as a wide variety of tubes and plates can be accommodated using the New Brunswick shaker accessories described in a later section (see Accessories on p. 58). These are easily accessed on slide-out platforms.

The Innova 44/44R may be operated in the following ways:

• Continuously: at a set speed and temperature, until user intervention.

• In a timed mode: run at a set speed, time and temperature for a period of up to 99.9 hours,

after which the shaker automatically shuts off.

• Via the shaker’s programmable controller: run through multiple temperature and speed

changes for an extended period of time.

For more details on these various modes of operation, (see Operation on p. 37).

For safe operation, the Innova 44/44R shakers are designed with a safety switch that automatically stops the shaker mechanism when the door is opened.

Biological Shakers Innova® 44/44R — Operating manual

The Innova 44/44R is equipped with visual and/or audible alarms that alert the user to the following conditions:

• The end of a timed run

• Deviations from speed setpoint

• Deviations from temperature setpoint

• Power failure

• Door open

• Unbalanced load

To accommodate customer needs, a wide variety of platforms can be used with the Innova 44/ 44R:

• Universal platforms are the most flexible, providing hole patterns for flask clamps, test tube

racks and other accessories.

• Dedicated platforms are supplied with flask clamps attached; they are designed solely and

expressly for this purpose.

• Test tube racks, microplate holders and test tube rack holders are also available (a universal

platform is needed for all test tube racks and holders).

For further information on these accessories, (see Accessories on p. 58).

Product description

Biological Shakers Innova® 44/44R — Operating manual

4 Inspection and unpacking of equipment

4 Inspection and unpacking of equipm ent

4.1 Inspection of boxes

After you receive your order from Eppendorf, inspect the boxes carefully for any damage that may have occurred during shipping. Report any damage immediately to the carrier and to your local Eppendorf Customer Service Department.

4.2 Packing list verification

Verify against your Eppendorf packing list that you have received all of the correct materials.

4.3 Unpacking of equipment

Risk of crushing due to missing auxiliary aid

 Lifting and transporting the shaker without suitable technical aids can result in crushing and

WARNING !

other injuries.

 Use a hydraulic lifting platform to install and uninstall the shaker.

To unpack your Innova 44/44R, you will need the following tools:

• Claw hammer

• Forklift or other lifting equipment to lift more than 259 kg (570 lb)

• Shears to cut 19 mm (3/4 in) wide steel strapping

• Tool to remove 7.6 cm (3 in) metal staples

Save all packing materials and this operating manual.

Inspection and unpacking of equipment

4.4 Out of box concerns

If any part of your order was damaged during shipping, is missing pieces, or fails to operate properly, please contact your Eppendorf sales representative.

4.5 Warranty registration

Please contact your Eppendorf Regional Sales Office.

Biological Shakers Innova® 44/44R — Operating manual

5 Preparing the location

5.1 Physical location

It is essential that the instrument be situated in an area where there is sufficient space for the shaker and platform to clear walls and potential obstructions during operation. The surface on which the unit is placed must be smooth, level, and able to support the shaker under full load operating conditions.

Risk of crushing due to missing auxiliary aid

 Lifting and transporting the shaker without suitable technical aids can result in crushing and

WARNING !

other injuries.

 Use a hydraulic lifting platform to install and uninstall the shaker.

5.2 Environment

The shaker is designed to operate optimally in the following ambient conditions:

• 10 °C to 35 °C

• 20 % to 80 % Relative Humidity (non-condensing)

5.3 Electrical requirements

The Innova 44/44R can be equipped to run on:

• 100 Volts, 50 Hz, 1500 VA maximum

• 100 Volts, 60 Hz, 1500 VA maximum

• 120 Volts, 60 Hz, 1500 VA maximum

• 230 Volts, 50 Hz, 1500 VA maximum

Check your shaker’s Electrical Specification Plate (located on the back of the unit) to be sure the electrical requirements of your unit match the output of your electric supply. If they do not match, contact your Eppendorf representative.

Preparing the location

5.4 Space requirements

Allow at least 10 cm (4 in) around the shaker for proper ventilation and for access to Power Switch and RS-232 port accessibility on the right side. Be sure to keep the power plug and power outlet easily accessible to facilitate unplugging the unit, as needed.

Biological Shakers Innova® 44/44R — Operating manual

Incuba

or Sha

ker Se

ries

Incubator Sha ker Se ries

NEW B

K SCIENTIFIC

NEW B

K SCIENTIFIC

Abb. 3: Innova 44/44R Front View Two Shakers Stacked on 12 in Base

Preparing the location

Fig. 3: Innova 44/44R Front View Two Shakers Stacked on 12 in Base

1 Width: 135 cm (53 in) 2 Depth: 84 cm (33 in) including front panel

protrusions

3 Medium Base Height: 30.5 cm (12 in) 4 Height: 66 cm (26 in) without feet

5 Height: 66 cm (26 in) without feet; Note: open door

adds 53 cm (21 in)

Be sure to allow at least 10 cm (4 in) around shaker for ventilation, access to power cord (rear

Hint!

panel), and access to power switch and RS-232 port (right side).

If you are stacking three shakers, use a 10 cm (4 in) base.

Biological Shakers Innova® 44/44R — Operating manual

6 Installation

Risk of crushing due to missing auxiliary aid

 Lifting and transporting the shaker without suitable technical aids can result in crushing and

WARNING !

Hint!

6.1 Tools required for installation

other injuries.

 Use a hydraulic lifting platform to install and uninstall the shaker.

Feet are used on the shaker ONLY when a single unit is operated without a base. At all other times (with two or three shakers stacked, or when a base is added), all feet must be removed.

To install the Innova 44/44R on an optional base and/or to stack Innova 44/44R units, the following tools will be needed:

• Number 2 Phillips head screwdriver

• Blade screwdriver

• 3 mm (1/8 in) Allen key

• 3/8 in Allen key (provided)

• Level, >25.4 cm (10 in)

• Two adjustable wrenches or 7/16 in socket wrench

• Forklift or other lifting equipment able to lift more than 259 kg (570 lb)

• Metal leveling shims (provided in stacking kit)

• Optional: set of screw-in lifting handles, for small lifts of short duration (adding leveling shims,

for example) or for minor location adjustments

• Optional: stacking kit(s)

Installation

6.2 Install the front grille

1. Remove and discard the plastic retaining clip that holds the drain hose in place for shipping.

2. Temporarily remove the thumbscrews (with their washers) installed at the bottom of each side panel, in the corner closest to the front of the shaker.

3. Hold the front grille in place (see Fig. 4 on p. 17) and reinstall the thumbscrews (and

Abb. 4: Install the Front Grille

Fig. 4: Install the Front Grille

1 Front Grille 2 Thumbscrews and Washers

washers).

6.3 Level a single shaker

These instructions are for a single shaker without an added base.

Make sure that the shaker is placed on a level surface and that all four feet are solidly on the surface. If the shaker is not level, adjust the feet as needed to achieve leveling:

Biological Shakers Innova® 44/44R — Operating manual

1. Immobilize the top locknut against the unit with one wrench whenever you adjust the foot, to

Abb. 5: Adjustable Foot (as shipped)

keep the threaded stud from falling out (see Fig. 5 on p. 18).

Installation

3.65 cm

Fig. 5: Adjustable Foot (as shipped)

1Bottom of Unit 2Locknut

3Flats for Wrench 4Foot

2. With a second wrench against the flats of the threaded stud, just above the foot, rotate clockwise to lower the foot or counter-clockwise to raise the foot.

3. Place a level on the top of the unit. If necessary, make further adjustments by repeating all steps until the unit is level.

4. After installing a platform (see Getting started on p. 32), fully load the shaker and do a test run at normal speed (see Operation on p. 37). Make additional leveling adjustments if necessary.

The maximum shaker lowering adjustment is 12.7 mm (1/2 in). The shaker cannot be raised

Hint!

above the height at which it was shipped. If more adjustment is required, you will need to add metal shims.

6.4 Level the optional base

Place the base on a sturdy (capable of bearing the weight of the combined base shakers and shaker contents), level surface, making sure that all four corners are solidly on the surface. If the base is not level, place metal shims as needed under the base until it is level.

6.5 Mount Innova 44/44R on optional base

1. Using a forklift or lifter, raise the Innova 44/44R so that its back end is tilted toward the rear of the base.

2. Remove all four feet from the bottom of the shaker. They will not be needed for stacking, but you may want to keep them for future use.

3. With two assistants guiding the shaker from opposite sides, lower the unit onto the base, back end first. Slowly and gently remove the forklift or lifter, lowering the front of the unit onto the base by hand.

4. Remove the set screws from the bottom sides of the shaker that correspond to the mounting holes for the rear corner braces (see Fig. 6 on p. 19).

Biological Shakers Innova® 44/44R — Operating manual

Abb. 6: Mount Shaker on Base

Installation

Fig. 6: Mount Shaker on Base

1 Innova 44/44R 2 Remove 1/2 – 13 Set Screws from holes that match

corner braces on both sides of unit.

3 1/2 – 13 x 1 1/4 in screw 4 Note: Feet removed

5. Using the 1/2 – 13 x 1 1/4 in screws, lock washers and washers provided, mount the rear braces on the base. Do not fully tighten the screws yet.

6. Mount the rear of the Innova 44/44R to the corner braces on the base, using the 1/2 – 13 x 1 1/4 in screws, lock washers and washers provided. Do not fully tighten the screws yet.

7. Mount the front corner braces to the base using two sets of the 1/2 – 13 x 1 1/4 in screws, lock washers and washers provided. Do not fully tighten the screws yet.

8. With the remaining 1/2 – 13 x 1 1/4 in screws, lockwashers and washers, attach the front corner braces to the unit.

9. Tighten all screws equally. With the corner braces securely in place, the shaker mounted on its base will look as shown (see Fig. 7 on p. 20):

Installation

Biological Shakers Innova® 44/44R — Operating manual

Abb. 7: Shaker Installed on Base

Fig. 7: Shaker Installed on Base

10. Make sure the shaker is level; add metal shims under the base as needed to level the unit. Shims are provided with the stacking kit.

11. After installing a platform (see Getting started on p. 32), fully load the shaker and do a test run at normal speed (see Start the shaker on p. 37). Make additional leveling adjustments if necessary.

6.6 Install the stacking kit

To stack two Innova 44/44R shakers, (see For two 44/44R shakers on p. 20). To stack an Innova 4400/4300 on an Innova 44/44R, (see For an Innova 4400/4430 on an Innova 44/44R on p. 21).

6.6.1 For two 44/44R shakers

1. Remove all feet from the units (put them aside for possible future use).

2. If you do not plan to use an optional base, skip to Step 3. If you are using one, level the base (see Level the optional base on p. 18), then mount the bottom unit on the base (see Mount Innova 44/44R on optional base on p. 18). If you are stacking three units, use a 4 in base.

3. With reference to the figure below (see Fig. 8 on p. 21), remove the set screws from the top of the bottom unit, on the two side edges, and remove the set screws (from the sides of the unit to be stacked) that correspond to the mounting brackets.

Biological Shakers Innova® 44/44R — Operating manual

Abb. 8: Install Innova 44/44R Stac king Kit

Installation

Fig. 8: Install Innova 44/44R Stacking Kit

1 Innova 44/44R 2 Remove 1/4 – 20 Set Screws before installing

stacking kit.

3 Remove set screws 4 Six sets of 1/4 – 20 x 3/4 in screws, lockwashers

and washers on each side.

4. Using the screws and washers provided, attach the stacking kit mounting brackets to the top of the bottom unit. Secure the brackets in place as shown (see Fig. 8 on p. 21).

6.6.2 For an Innova 4400/4430 on an Innova 44/44R

1. Remove all feet from the units (put them aside for possible future use).

2. If you do not plan to use an optional base, skip to Step 3. If you are using one, level the base (see Level the optional base on p. 18), then mount the bottom unit on the base as explained in Section above (see Mount Innova 44/44R on optional base on p. 18). If you are stacking three units, use a 4 in base.

3. With reference to the figure below (see Fig. 9 on p. 22), remove the screws installed at the top of the bottom unit, on the two side edges.

Installation

Biological Shakers Innova® 44/44R — Operating manual

Abb. 9: Install 4400/4430 to 44/4 4R Stacking Kit

Fig. 9: Install 4400/4430 to 44/44R Stacking Kit

1 Innova 4400/4430 2 Install right bracket with three 1/4 – 20 x 3/4 in

flathead socket screws, with two drops of Loctite on the threads.

3 Remove screws before installing mounting

bracket.

5 Install left bracket with two sets of 10 – 32 x 1/2 in

screws, lockwashers and washers and three sets of 1/4 – 20 x 3/4 in screws, lockwashers and washers. Allow some play until top unit is secure against right bracket, then tighten.

4. Use the countersunk flathead screws provided, with two drops of Loctite on their threads, to attach the righthand (facing the door) mounting bracket to the top of the unit installed on the base. Secure the bracket in place.

5. Use the other screws and washers provided to attach the lefthand mounting bracket to the top of the unit installed on the base. Loosely tighten these screws, to allow some play.

6.7 Stack two Innova 44/44R shakers

1. Using a forklift or lifter, raise the Innova 44/44R to be stacked so that its back end is tilted toward the rear of the mounting brackets.

2. Remove the feet from the unit. They will not be needed for stacking, but you may want to keep them for future use.

4 Optional Base

+ 50 hidden pages