Eppendorf 44R User Manual

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Operating manual

Draft

—

Biological Shakers Innova® 44/44R

see

on p.Fig.Tab.p

erating manua

M1282-0050

New Brunswick™ Biological Shakers

Innova® 44/44R

Operating manual

Eppendorf

and the Eppendorf logo are registered trademarks of Eppendorf AG, Germany

New Brunswick™ and the New Brunswick™ logo are trademarks of Eppendorf AG, Germany.

Innova® is a registered trademark of New Brunswick Scientific Co., Inc., USA.

Windows

is a registered trademark of Microsoft Corporation in the United States and other countries.

Trademarks are not marked in all cases with ™ or ® in this manual.

M1282-0050-N/022013



Biological Shakers Innova® 44/44R — Operating manual

Table of contents

1 Table of contents

1 User instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.1 Hazard symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.3 Application limits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.3.1 Description of ATEX Guideline (94/9EC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.5 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.5.1 Personal injury and damage to device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.5.2 Incorrect handling of accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3 Product description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.1 Main illustration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.2 General overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

4 Inspection and unpacking of equipment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.1 Inspection of boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.2 Packing list verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.3 Unpacking of equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.4 Out of box concerns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.5 Warranty registration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Table of contents

5 Preparing the location. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5.1 Physical location. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5.2 Environment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5.3 Electrical requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5.4 Space requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

6 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

6.1 Tools required for installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

6.2 Install the front grille . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

6.3 Level a single shaker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

6.4 Level the optional base. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

6.5 Mount Innova 44/44R on optional base . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

6.6 Install the stacking kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

6.6.1 For two 44/44R shakers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

6.6.2 For an Innova 4400/4430 on an Innova 44/44R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

6.7 Stack two Innova 44/44R shakers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

6.8 Stack a 4400/4430 on a 44/44R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

6.9 Stack a third shaker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

7 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

7.1 Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

7.2 LCD display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Table of contents

Biological Shakers Innova® 44/44R — Operating manual

7.3 Changing screens. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

7.4 Display icons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

7.5 Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

7.5.1 Optional remote alarm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

7.6 Glide-up door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.7 Spill pan/water reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.8 Software interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.9 Interior light(s). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.10 Heater. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.11 Refrigeration (44R only) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

7.12 Service accessibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

8 Getting started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

8.1 Platform assemblies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

8.2 Installation of platform. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

8.3 Install flask clamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

8.4 Electrical connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

8.5 Optional gassing manifold kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.6 Optional UV germicidal lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.7 Optional photosynthetic lamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.8 Optional humidity monitor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.9 Fill the water reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

8.10 Drain the water reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

9 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

9.1 Safety precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

9.2 Open the door. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

9.3 Start the shaker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

9.4 Using the LCD screens. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

9.4.1 Display screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

9.4.2 Summary screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

9.4.3 Setup screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

9.4.4 Lamps screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

9.4.5 RS232 Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

9.4.6 Calibrate screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

9.4.7 Programs screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

9.5 Program the shaker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

9.5.1 Timer only . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

9.5.2 Programmed steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

9.5.3 Create a program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

9.5.4 Edit a program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

9.5.5 Run a program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

9.6 Mute the audible alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

9.7 Temperature offset calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

9.7.1 Calculate the offset value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

9.7.2 Set the offset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

9.8 Using Calspeed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

9.9 Power interruption. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

9.10 Slide-out platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

10 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

10.1 General troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

11 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

11.1 Routine maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

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Biological Shakers Innova® 44/44R — Operating manual

11.2 Cleaning external and internal surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

11.3 Biohazard decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

12 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

12.1 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

13 Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

13.1 Replacement parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

13.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

13.2.1 Slide-out platforms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

13.2.2 Flask clamps for universal platforms. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

13.2.3 Replacement clamp hardware kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

13.2.4 Test tube racks and other accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

13.2.5 Optional bases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

13.2.6 Optional stacking kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

13.2.7 Optional handles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

14 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

14.1 Transport and storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

14.2 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

15 Appendix A: Remote programming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

15.1 Setting up with HyperTerminal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

15.2 Overview of command sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

15.3 Index to command codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

15.4 Set commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

15.5 Profile control commands. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

15.6 Report request commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

15.7 Set/Get date and time. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

Table of contents

16 Appendix B: Certifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

16.1 Certifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

Biological Shakers Innova® 44/44R — Operating manual

Table of contents

Biological Shakers Innova® 44/44R — Operating manual

1 User instructions

1.1 Using this manual



Carefully read this operating manual before using the device for the first time.

 Also observe the operating manual enclosed with the accessories.

 The operating manual should be considered as part of the product and stored in a location

that is easily accessible.

 When passing the device on to third parties, be sure to include this operating manual.

 If this manual is lost, please request another one. The current version can be found on our

website www.eppendorf.com.

1.2 Danger symbols and danger levels

1.2.1 Hazard symbols

Hazard point Burns

Electric shock Material damage

User instructions

Explosion Heavy loads

Inhalation

1.2.2 Degrees of danger

The following degree levels are used in safety messages throughout this manual. Acquaint yourself with each item and the potential risk if you disregard the safety message.

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.

CAUTION May lead to light to moderate injuries.

NOTICE May lead to material damage.

1.3 Symbols used

Example Meaning

 You are requested to perform an action.

Perform these actions in the sequence described.

• List.

References useful information.

Safety

Biological Shakers Innova® 44/44R — Operating manual

2 Safety

2Safety

2.1 Intended use

This device is exclusively intended for indoor use and for uniform movement and temperature control of biological solutions and cultures in reaction vessels.

Lack of safety due to incorrect accessories or spare parts

Accessories and spare parts that are not recommended by Eppendorf compromise the safety,

CAUTION!

2.2 User profile

2.3 Application limits

2.3.1 Description of ATEX Guideline (94/9EC)

function and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of non-recommended accessories and spare parts.

 Only use accessories and original spare parts recommended by Eppendorf.

The device may only be operated by trained lab personnel who have carefully read this operating manual and are familiar with the device functions.

Explosion hazard

 Do not operate the device in areas where work is completed with explosive substances.

DANGER!

 Do not use this device to process any explosive or highly reactive substances.

 Do not use this device to process any substances which could create an explosive

atmosphere.

Due to its design and the ambient conditions in its interior, the device is not suitable for use in potentially explosive atmospheres.

The device may only be used in a safe environment, e.g., the open atmosphere of a ventilated lab.

The use of substances which may contribute to a potentially explosive atmosphere is not permitted.

The final decision regarding the risks associated with using these types of substances is the user's responsibility.

2.4 Information on product liability

In the following cases, the designated protection of the device may be compromised.

The liability for the function of the device passes to the operator if:

• The device is not used in accordance with this operating manual.

• The device is used outside of the range of application described in the succeding chapters.

• The device is used with accessories or consumables that were not approved by Eppendorf.

• Service or maintenance is completed on the device by people who are not authorized by

Eppendorf.

• The owner has made unauthorized modifications to the device.

2.5 Warnings for intended use

Before using the device, read this operating manual and observe the following general safety instructions.

Biological Shakers Innova® 44/44R — Operating manual

2.5.1 Personal injury and damage to device

Electric shock due to damage to the device or power cable

 Only switch on the device if the device and power cable are undamaged.

WARNING !

 Only use devices that have been properly installed or repaired.

Lethal voltages inside of the device

 Ensure that the housing is always closed and undamaged so the user cannot accidentally

touch the parts inside.

 Do not remove the housing of the device.

Danger due to incorrect power supply

 Only connect the device to voltage sources that meet the requirements on the name plate.

 Only use sockets with a protective earth (PE) conductor and suitable power cable.

Health risk due to contact with infectious liquids and pathogenic bacteria

 Observe the national regulations for handling these substances, the biological security level

of your laboratory, the material safety data sheets and the manufacturer's application notes.

 Wear personal protective equipment (PPE).

 Follow the instructions regarding hygiene, cleaning and decontamination.

 For complete instructions on the handling of germs or biological material in risk group II or

higher, please refer to the "Laboratory Biosafety Manual" (source: World Health Organization).

Safety

WARNING !

CAUTION!

NOTICE!

Health risk due to poisonous, radioactive or aggressive chemicals

 Observe the national regulations for handling these substances as well as the material safety

data sheets and manufacturer's application notes.

 Wear personal protective equipment (PPE).

Burns due to hot metal on the device and hot flasks

 Only touch the device and flasks when wearing protective gloves.

Risk of crushing due to missing auxiliary aid

 Lifting and transporting the shaker without suitable technical aids can result in crushing and

other injuries.

 Use a hydraulic lifting platform to install and uninstall the shaker.

Risk to health due to lifting heavy loads

 Only lift the device with another person or using a suitable aid.

 Make sure to use a transport aid for transportation over long distances.

Damage to electronic components due to condensation

Condensation can form inside the device after the device has been moved from a cool to a warmer environment.

 Wait at least three hours before connecting it to the mains/power supply.

Safety

Biological Shakers Innova® 44/44R — Operating manual

2.5.2 Incorrect handling of accessories

Lack of safety due to incorrect accessories or spare parts

Accessories and spare parts that are not recommended by Eppendorf compromise the safety,

CAUTION!

NOTICE!

function and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of non-recommended accessories and spare parts.

 Only use accessories and original spare parts recommended by Eppendorf.

Danger due to damaged tubes

Small scratches and cracks can cause severe damage to the device and accessories. Liquids may escape.

 Before use, visually check all tubes for any damage.

 Never use damaged tubes.

Biological Shakers Innova® 44/44R — Operating manual

Incuba

or Shaker Series

NEW BRUNSWICK SCIENTIFIC

7321456

11 10 9 8

3 Product description

3.1 Main illustration

Abb. 1: Front and rear view of the In nova 44/44R

Fig. 1: Front and rear view of the Innova 44/44R

1Door

With automatic stop function

2 Door handle

Product description

3 Start/Stop switch

Start or stop drive

5 Push button control knob

Set or change the parameters or Start or stop drive

7 Name plate

Model number, documentation number, serial number and electrical connection data

9 Mains power connection

Connect the power cable

11 Discharge hose

Discharge hose with clamp

4Display

Graphical user interface with display of parameters and parameter values

6 Mains power switch

Switch device on or off

8 Base stand

Align the device horizontally.

10 RS-232 interface

Read out parameter values and control operational functions using computer applications

Biological Shakers Innova® 44/44R — Operating manual

Abb. 2: Internal view

1234

Fig. 2: Internal view

1 Halogen lighting 2 Drive

Eccentric drive - orbit is model-dependent.

3 Drip pan reservoir

Catch liquids and store water

4 Platform frame

Remove the platform and platform frame.

3.2 General overview

The Innova 44/44R stackable incubator and incubator refrigerated shakers are large-capacity

Product description

orbital shakers that utilize a triple eccentric counter-balanced drive mechanism. They provide horizontal plane rotary motion in either a 2.54 cm (1 in) or a 5 cm (2 in) diameter circular orbit, depending on the model. A Proportional/Integral (PI) microprocessor controller with instantaneous digital feedback controls the speed over the entire speed range.

The Innova 44R provides temperature control from 20 °C below ambient (as low as 4 °C) to 80 °C, and the Innova 44 from 5 °C above ambient to 80 °C. Naturally, both these ranges depend on relative humidity and other ambient factors, as well as the options installed in the unit. Ambient temperature is measured at one meter from the exterior of the unit.

Erlenmeyer flasks (up to 5 liters in size) as well as a wide variety of tubes and plates can be accommodated using the New Brunswick shaker accessories described in a later section (see Accessories on p. 58). These are easily accessed on slide-out platforms.

The Innova 44/44R may be operated in the following ways:

• Continuously: at a set speed and temperature, until user intervention.

• In a timed mode: run at a set speed, time and temperature for a period of up to 99.9 hours,

after which the shaker automatically shuts off.

• Via the shaker’s programmable controller: run through multiple temperature and speed

changes for an extended period of time.

For more details on these various modes of operation, (see Operation on p. 37).

For safe operation, the Innova 44/44R shakers are designed with a safety switch that automatically stops the shaker mechanism when the door is opened.

Biological Shakers Innova® 44/44R — Operating manual

The Innova 44/44R is equipped with visual and/or audible alarms that alert the user to the following conditions:

• The end of a timed run

• Deviations from speed setpoint

• Deviations from temperature setpoint

• Power failure

• Door open

• Unbalanced load

To accommodate customer needs, a wide variety of platforms can be used with the Innova 44/ 44R:

• Universal platforms are the most flexible, providing hole patterns for flask clamps, test tube

racks and other accessories.

• Dedicated platforms are supplied with flask clamps attached; they are designed solely and

expressly for this purpose.

• Test tube racks, microplate holders and test tube rack holders are also available (a universal

platform is needed for all test tube racks and holders).

For further information on these accessories, (see Accessories on p. 58).

Product description

Biological Shakers Innova® 44/44R — Operating manual

4 Inspection and unpacking of equipment

4 Inspection and unpacking of equipm ent

4.1 Inspection of boxes

After you receive your order from Eppendorf, inspect the boxes carefully for any damage that may have occurred during shipping. Report any damage immediately to the carrier and to your local Eppendorf Customer Service Department.

4.2 Packing list verification

Verify against your Eppendorf packing list that you have received all of the correct materials.

4.3 Unpacking of equipment

Risk of crushing due to missing auxiliary aid

 Lifting and transporting the shaker without suitable technical aids can result in crushing and

WARNING !

other injuries.

 Use a hydraulic lifting platform to install and uninstall the shaker.

To unpack your Innova 44/44R, you will need the following tools:

• Claw hammer

• Forklift or other lifting equipment to lift more than 259 kg (570 lb)

• Shears to cut 19 mm (3/4 in) wide steel strapping

• Tool to remove 7.6 cm (3 in) metal staples

Save all packing materials and this operating manual.

Inspection and unpacking of equipment

4.4 Out of box concerns

If any part of your order was damaged during shipping, is missing pieces, or fails to operate properly, please contact your Eppendorf sales representative.

4.5 Warranty registration

Please contact your Eppendorf Regional Sales Office.

Biological Shakers Innova® 44/44R — Operating manual

5 Preparing the location

5.1 Physical location

It is essential that the instrument be situated in an area where there is sufficient space for the shaker and platform to clear walls and potential obstructions during operation. The surface on which the unit is placed must be smooth, level, and able to support the shaker under full load operating conditions.

Risk of crushing due to missing auxiliary aid

 Lifting and transporting the shaker without suitable technical aids can result in crushing and

WARNING !

other injuries.

 Use a hydraulic lifting platform to install and uninstall the shaker.

5.2 Environment

The shaker is designed to operate optimally in the following ambient conditions:

• 10 °C to 35 °C

• 20 % to 80 % Relative Humidity (non-condensing)

5.3 Electrical requirements

The Innova 44/44R can be equipped to run on:

• 100 Volts, 50 Hz, 1500 VA maximum

• 100 Volts, 60 Hz, 1500 VA maximum

• 120 Volts, 60 Hz, 1500 VA maximum

• 230 Volts, 50 Hz, 1500 VA maximum

Check your shaker’s Electrical Specification Plate (located on the back of the unit) to be sure the electrical requirements of your unit match the output of your electric supply. If they do not match, contact your Eppendorf representative.

Preparing the location

5.4 Space requirements

Allow at least 10 cm (4 in) around the shaker for proper ventilation and for access to Power Switch and RS-232 port accessibility on the right side. Be sure to keep the power plug and power outlet easily accessible to facilitate unplugging the unit, as needed.

Biological Shakers Innova® 44/44R — Operating manual

Incuba

or Sha

ker Se

ries

Incubator Sha ker Se ries

NEW B

K SCIENTIFIC

NEW B

K SCIENTIFIC

Abb. 3: Innova 44/44R Front View Two Shakers Stacked on 12 in Base

Preparing the location

Fig. 3: Innova 44/44R Front View Two Shakers Stacked on 12 in Base

1 Width: 135 cm (53 in) 2 Depth: 84 cm (33 in) including front panel

protrusions

3 Medium Base Height: 30.5 cm (12 in) 4 Height: 66 cm (26 in) without feet

5 Height: 66 cm (26 in) without feet; Note: open door

adds 53 cm (21 in)

Be sure to allow at least 10 cm (4 in) around shaker for ventilation, access to power cord (rear

Hint!

panel), and access to power switch and RS-232 port (right side).

If you are stacking three shakers, use a 10 cm (4 in) base.

Biological Shakers Innova® 44/44R — Operating manual

6 Installation

Risk of crushing due to missing auxiliary aid

 Lifting and transporting the shaker without suitable technical aids can result in crushing and

WARNING !

Hint!

6.1 Tools required for installation

other injuries.

 Use a hydraulic lifting platform to install and uninstall the shaker.

Feet are used on the shaker ONLY when a single unit is operated without a base. At all other times (with two or three shakers stacked, or when a base is added), all feet must be removed.

To install the Innova 44/44R on an optional base and/or to stack Innova 44/44R units, the following tools will be needed:

• Number 2 Phillips head screwdriver

• Blade screwdriver

• 3 mm (1/8 in) Allen key

• 3/8 in Allen key (provided)

• Level, >25.4 cm (10 in)

• Two adjustable wrenches or 7/16 in socket wrench

• Forklift or other lifting equipment able to lift more than 259 kg (570 lb)

• Metal leveling shims (provided in stacking kit)

• Optional: set of screw-in lifting handles, for small lifts of short duration (adding leveling shims,

for example) or for minor location adjustments

• Optional: stacking kit(s)

Installation

6.2 Install the front grille

1. Remove and discard the plastic retaining clip that holds the drain hose in place for shipping.

2. Temporarily remove the thumbscrews (with their washers) installed at the bottom of each side panel, in the corner closest to the front of the shaker.

3. Hold the front grille in place (see Fig. 4 on p. 17) and reinstall the thumbscrews (and

Abb. 4: Install the Front Grille

Fig. 4: Install the Front Grille

1 Front Grille 2 Thumbscrews and Washers

washers).

6.3 Level a single shaker

These instructions are for a single shaker without an added base.

Make sure that the shaker is placed on a level surface and that all four feet are solidly on the surface. If the shaker is not level, adjust the feet as needed to achieve leveling:

Biological Shakers Innova® 44/44R — Operating manual

1. Immobilize the top locknut against the unit with one wrench whenever you adjust the foot, to

Abb. 5: Adjustable Foot (as shipped)

keep the threaded stud from falling out (see Fig. 5 on p. 18).

Installation

3.65 cm

Fig. 5: Adjustable Foot (as shipped)

1Bottom of Unit 2Locknut

3Flats for Wrench 4Foot

2. With a second wrench against the flats of the threaded stud, just above the foot, rotate clockwise to lower the foot or counter-clockwise to raise the foot.

3. Place a level on the top of the unit. If necessary, make further adjustments by repeating all steps until the unit is level.

4. After installing a platform (see Getting started on p. 32), fully load the shaker and do a test run at normal speed (see Operation on p. 37). Make additional leveling adjustments if necessary.

The maximum shaker lowering adjustment is 12.7 mm (1/2 in). The shaker cannot be raised

Hint!

above the height at which it was shipped. If more adjustment is required, you will need to add metal shims.

6.4 Level the optional base

Place the base on a sturdy (capable of bearing the weight of the combined base shakers and shaker contents), level surface, making sure that all four corners are solidly on the surface. If the base is not level, place metal shims as needed under the base until it is level.

6.5 Mount Innova 44/44R on optional base

1. Using a forklift or lifter, raise the Innova 44/44R so that its back end is tilted toward the rear of the base.

2. Remove all four feet from the bottom of the shaker. They will not be needed for stacking, but you may want to keep them for future use.

3. With two assistants guiding the shaker from opposite sides, lower the unit onto the base, back end first. Slowly and gently remove the forklift or lifter, lowering the front of the unit onto the base by hand.

4. Remove the set screws from the bottom sides of the shaker that correspond to the mounting holes for the rear corner braces (see Fig. 6 on p. 19).

Biological Shakers Innova® 44/44R — Operating manual

Abb. 6: Mount Shaker on Base

Installation

Fig. 6: Mount Shaker on Base

1 Innova 44/44R 2 Remove 1/2 – 13 Set Screws from holes that match

corner braces on both sides of unit.

3 1/2 – 13 x 1 1/4 in screw 4 Note: Feet removed

5. Using the 1/2 – 13 x 1 1/4 in screws, lock washers and washers provided, mount the rear braces on the base. Do not fully tighten the screws yet.

6. Mount the rear of the Innova 44/44R to the corner braces on the base, using the 1/2 – 13 x 1 1/4 in screws, lock washers and washers provided. Do not fully tighten the screws yet.

7. Mount the front corner braces to the base using two sets of the 1/2 – 13 x 1 1/4 in screws, lock washers and washers provided. Do not fully tighten the screws yet.

8. With the remaining 1/2 – 13 x 1 1/4 in screws, lockwashers and washers, attach the front corner braces to the unit.

9. Tighten all screws equally. With the corner braces securely in place, the shaker mounted on its base will look as shown (see Fig. 7 on p. 20):

Installation

Biological Shakers Innova® 44/44R — Operating manual

Abb. 7: Shaker Installed on Base

Fig. 7: Shaker Installed on Base

10. Make sure the shaker is level; add metal shims under the base as needed to level the unit. Shims are provided with the stacking kit.

11. After installing a platform (see Getting started on p. 32), fully load the shaker and do a test run at normal speed (see Start the shaker on p. 37). Make additional leveling adjustments if necessary.

6.6 Install the stacking kit

To stack two Innova 44/44R shakers, (see For two 44/44R shakers on p. 20). To stack an Innova 4400/4300 on an Innova 44/44R, (see For an Innova 4400/4430 on an Innova 44/44R on p. 21).

6.6.1 For two 44/44R shakers

1. Remove all feet from the units (put them aside for possible future use).

2. If you do not plan to use an optional base, skip to Step 3. If you are using one, level the base (see Level the optional base on p. 18), then mount the bottom unit on the base (see Mount Innova 44/44R on optional base on p. 18). If you are stacking three units, use a 4 in base.

3. With reference to the figure below (see Fig. 8 on p. 21), remove the set screws from the top of the bottom unit, on the two side edges, and remove the set screws (from the sides of the unit to be stacked) that correspond to the mounting brackets.

Biological Shakers Innova® 44/44R — Operating manual

Abb. 8: Install Innova 44/44R Stac king Kit

Installation

Fig. 8: Install Innova 44/44R Stacking Kit

1 Innova 44/44R 2 Remove 1/4 – 20 Set Screws before installing

stacking kit.

3 Remove set screws 4 Six sets of 1/4 – 20 x 3/4 in screws, lockwashers

and washers on each side.

4. Using the screws and washers provided, attach the stacking kit mounting brackets to the top of the bottom unit. Secure the brackets in place as shown (see Fig. 8 on p. 21).

6.6.2 For an Innova 4400/4430 on an Innova 44/44R

1. Remove all feet from the units (put them aside for possible future use).

2. If you do not plan to use an optional base, skip to Step 3. If you are using one, level the base (see Level the optional base on p. 18), then mount the bottom unit on the base as explained in Section above (see Mount Innova 44/44R on optional base on p. 18). If you are stacking three units, use a 4 in base.

3. With reference to the figure below (see Fig. 9 on p. 22), remove the screws installed at the top of the bottom unit, on the two side edges.

Installation

Biological Shakers Innova® 44/44R — Operating manual

Abb. 9: Install 4400/4430 to 44/4 4R Stacking Kit

Fig. 9: Install 4400/4430 to 44/44R Stacking Kit

1 Innova 4400/4430 2 Install right bracket with three 1/4 – 20 x 3/4 in

flathead socket screws, with two drops of Loctite on the threads.

3 Remove screws before installing mounting

bracket.

5 Install left bracket with two sets of 10 – 32 x 1/2 in

screws, lockwashers and washers and three sets of 1/4 – 20 x 3/4 in screws, lockwashers and washers. Allow some play until top unit is secure against right bracket, then tighten.

4. Use the countersunk flathead screws provided, with two drops of Loctite on their threads, to attach the righthand (facing the door) mounting bracket to the top of the unit installed on the base. Secure the bracket in place.

5. Use the other screws and washers provided to attach the lefthand mounting bracket to the top of the unit installed on the base. Loosely tighten these screws, to allow some play.

6.7 Stack two Innova 44/44R shakers

1. Using a forklift or lifter, raise the Innova 44/44R to be stacked so that its back end is tilted toward the rear of the mounting brackets.

2. Remove the feet from the unit. They will not be needed for stacking, but you may want to keep them for future use.

4 Optional Base

Biological Shakers Innova® 44/44R — Operating manual

Incuba

or Sha

ker Se

ries

Incubator Sha ker Se ries

NEW B

K SCIENTIFIC

NEW B

K SCIENTIFIC

3. With two assistants guiding the unit from opposite sides, lower the shaker onto the mounting brackets, back end first. Slowly and gently remove the forklift or lifter, lowering the front of the unit onto the mounting brackets by hand.

4. As shown (see Fig. 10 on p. 23), secure the bottom of the upper shaker to the mounting brackets (already fastened to the shaker below) with the screws, lock washers and washers provided.

Abb. 10: Stacked Sha kers

Installation

Fig. 10: Stacked Shakers

5. Make sure the shakers are level; add metal shims under the bottom unit (or base) if needed.

6. After installing a platform (see Getting started on p. 32), fully load the shaker and do a test run

If you are stacking a third shaker, (see Stack a third shaker on p. 24).

at normal speed (see Start the shaker on p. 37). Make additional leveling adjustments if necessary.

Installation

Biological Shakers Innova® 44/44R — Operating manual

6.8 Stack a 4400/4430 on a 44/44R

1. Using a forklift or lifter, raise the Innova 4400/4430 to be stacked so that its back end is tilted toward the rear of the mounting brackets.

2. Remove the feet from the unit. They will not be needed for stacking, but you may want to keep them for future use.

4. As indicated (see Fig. 9 on p. 22), secure the bottom of the upper shaker to the mounting brackets with the screws, lockwashers and washers provided. Be sure to tighten the right side first, then secure the left side.

5. Make sure the shakers are level; add metal shims under the base if needed.

6.9 Stack a third shaker

Equipment damage

 When stacking three shakers, do not use a base taller than 10.5 cm (4 in).

CAUTION!

 When stacking three shakers, all shaker loads must be balanced. These shakers operate

best at maximum speed with a load of 15.5 ( ± 1.4) kg or 34 (± 3) lb, which includes all platforms, clamps, and filled glassware.

 For three stacked 2 in stroke shakers, maximum operating speed must be limited to 250 rpm.

To stack a third shaker, repeat the appropriate procedures provided above to install the stacking kit, to stack the shaker, and to level the entire assembly.

Biological Shakers Innova® 44/44R — Operating manual

7Features

7.1 Controls

Abb. 11: Front Pane l (detail)

Fig. 11: Front Panel (detail)

1 Control Knob 2 Display

3 Start/Stop Switch (for shaking)

Features

• START/STOP SWITCH: This switch is used to start or stop the shaker. It will also activate the

timer when a timed run is desired. If the unit is stopped and restarted, the timer automatically returns to the beginning of a run.

• CONTROL KNOB: This knob is multifunctional. It is used to change screens and to select

and change operating conditions.

• RS-232 PORT: For details (see Software interfaces on p. 31).

• POWER SWITCH: This rocker switch is a circuit breaker that turns power on and off to the

entire Innova 44/44R.

In addition to the Power Switch, the Power Cord is also used to conduct power or to break the

Hint!

power circuit to the shaker. Whenever power to the shaker may be a hazard (during cleaning, maintenance or service work), be sure to disconnect the power cord from the electrical outlet.

Biological Shakers Innova® 44/44R — Operating manual

Abb. 12: Contr ol Panel (Right Side)

Features

Fig. 12: Control Panel (Right Side)

1 Power Switch 2 RS-232 Port

3 Front of Shaker 4 Control Knob

7.2 LCD display

When the unit is powered up, using the Power Switch located on the lower right side of the machine (see Fig. 12 on p. 26), the initial display screen will remain for a short time while the system boots up. Then the main screen, called DISP for Display, appears (see Fig. 13 on p. 27). This screen will indicate the same parameters that were in effect when the power was turned off.

Biological Shakers Innova® 44/44R — Operating manual

ºC 23

RPM 120

DISP We 13 : 44

Abb. 13: Display Scr een

Features

Fig. 13: Display Screen

1 Actual Parameter values 2 Day and 24-Hour Time

3 Program Running 4 Heater On

5 Door Open 6 Parameters Locked

7 Audible Alarm Muted 8 Screen Name

9 Parameters 10 Temperature Offset

• For an explanation of the icons on the display screen, (see Display icons on p. 29).

• Turning the Control Knob will highlight functions and/or values that can be changed.

• Whenever you turn the Control Knob, the chamber light will go on (see Interior light(s) on

p. 31).

For more information on working in the Display Screen, (see Using the LCD screens on p. 37).

7.3 Changing screens

You can change screens displayed by highlighting the screen name field in the lower left corner, pressing the Control Knob in until it clicks, rotating the Knob left or right (which also makes clicking sounds) to the desired screen and clicking the Control Knob in again. The table below describes the various screens:

Biological Shakers Innova® 44/44R — Operating manual

Tab. 1: Screens

Screen Name Meaning Features/Modes

Features

DISP Display

SUMM Summary

SET Set-Up

LAMP Lamps

COMM Communication (RS-232)

CAL Calibration

PROG Program Allows user to set up 1 – 4 programs, each with 1 – 15 steps.

See table below, Display Screen Parameters; 2 Optional; 3 Not installed

Shows two user-selectable parameters1 and actual values.

Shows all parameters1, setpoints and actual values.

Set day of week, set time, enable or mute alarm, lock or unlock operating parameters

Internal chamber light: ON (always on); OFF (always off); AUTO (default mode), light goes on and stays on when door is open, shuts off 15 seconds after door is closed, and goes on for 15 seconds when Control Knob is moved. Photosynthetic lights

: ON, OFF, NONE3, UV Light (UV)2: ON, OFF, NONE3

(GRO)

SET: set baud rate OFF disables RS-232 MONITOR: PC commands shaker to read setpoints and actual values on a schedule determined by PC software. Parameters are unlocked and can be changed by program or manually. SLAVE: PC controls shaker and logs data. TALK: Shaker sends setpoint and actual data to PC at one-minute intervals.

Allows user to enter a temperature offset. Allows user to calibrate speed.

Biological Shakers Innova® 44/44R — Operating manual

Tab. 2: Display Screen Parameters

Parameter Name Meaning

RPM Shaking speed, in revolutions/minute

°C Chamber temperature, in degrees Celsius

HRS Programmed time remaining, in hours

%RH

GRO

Optional

Relative Humidity, in percent.

Status of Ultraviolet germicidal lamp

Status of Photosynthetic growth lamps

7.4 Display icons

Features

There are six icons that help identify operating conditions (see table below, Display Icons). Five of these icons are located at the bottom of the display, and they are visible, when applicable, in any screen you are viewing, except the Program subscreens. The sixth appears, when applicable, next to °C whenever the temperature is onscreen.

Biological Shakers Innova® 44/44R — Operating manual

Tab. 3: Display Icons

Icon Explanation

This icon appears when audible alarms are muted.

This icon appears when the possibility to make manual or programmed parameter changes is disabled (locked). This is controlled by settings on the SET screen (see Setup screen on p. 40).

Features

7.5 Alarms

Indication Description

POWER

TEMP

RPM

This icon appears when the shaker door is open.

This icon appears when the Heater is on (see Heater on p. 31).

This icon appears when a user-defined Program is running (see Program the shaker on p. 45).

This icon appears to the right of °C if the Temperature Offset feature is being used (see Temperature offset calibration on p. 49).

If an alarm condition exists, the field in the lower right corner will alternate the Day and Time with characters indicating the nature of the alarm condition, accompanied by an audible alarm (unless muted) (see Table below):

The temperature deviates more than ±1 °C from setpoint after achieving control temperature range. After door is opened, alarm will be disabled for 5 minutes while chamber recovers to setpoint.

The speed deviates more than ±5 RPM from setpoint after achieving operating speed setpoint. After door is opened, alarm will be disabled for 5 minutes while chamber recovers to setpoint.

Indicates unit is powering up (both at normal power-up and after power interruption); will flash until the Control Knob is moved.

HRS Indicates when timed run is completed.

TILT

7.5.1 Optional remote alarm

Indicates an unbalanced shaking condition. After load is evenly distributed and/or shaker is leveled, restart by pressing START/STOP.

The Innova 44/44R can be equipped with a factory-installed remote alarm component (part number M1320-8029). When it is hooked up to your relay and receiving equipment, this device will send notification of an alarm condition to the remote location you choose.

Biological Shakers Innova® 44/44R — Operating manual

7.6 Glide-up door

The Innova 44/44R is equipped with a space-saving glide-up door.

When the door opens, the following will happen:

• Heater turns off

• Shaker stops

• Interior light goes on and, when it is in AUTO mode, will remain on for 15 seconds after the

door is closed

• UV germicidal lamp (if so equipped) turns off

• Photosynthetic lights (if so equipped) turn off.

7.7 Spill pan/water reservoir

The Innova 44/44R is equipped with a spill pan to protect the drive mechanism in the case of accidental spills and/or broken glassware. This pan can also be used as a water reservoir to humidify the chamber and to reduce evaporation. An optional factory-installed humidity monitor is also available.

7.8 Software interfaces

The RS-232 port is located below the Power Switch on the right side of the control panel (see Fig. 12 on p. 26). It can be used to interface a PC to the shaker for control of operating conditions or data logging applications (see Appendix A: Remote programming on p. 64).

The customer is responsible for securing the proper driver to interface with the RS-232.

Features

7.9 Interior light(s)

When the LAMP screen is in its default AUTO mode, the interior (“chamber”) light is activated for 15 seconds whenever you turn the Control Knob. It will automatically shut off after 15 seconds of Control Knob inactivity.

The chamber light will also go on when the door is open.

In addition, you can set the chamber light to be continuously ON or OFF by selecting either mode in the LAMP screen.

There are two additional light options for refrigerated units only: interior photosynthetic growth lamps (see Optional photosynthetic lamps on p. 35) and a UV germicidal lamp located outside the chamber but in the airflow path (see Optional UV germicidal lamp on p. 35).

7.10 Heater

The chamber temperature is sensed by a 1000 ohm platinum RTD. The heater is controlled using pulse width modulation on a 2.5 s duty cycle. This cycle time is fast enough to prevent noticeable changes in air temperature due to the cycling.

Whenever the heater is on, the Heater On icon will appear in the display. The heater automatically stops running when the door is opened.

7.11 Refrigeration (44R only)

The refrigeration system in the Innova 44R is a fixed-capacity system carefully designed to maintain the setpoint, to balance pressure within the system, and to prevent freezing on the evaporator surface.

When the shaker is powered up, there is a four-minute time delay prior to compressor start-up.

7.12 Service accessibility

In the unlikely event that your Innova 44/44R should need service, all electronic boards, refrigeration and heating components are mounted on a pull-out drawer mechanism which is easily accessed, by an authorized service technician, from the front of the shaker.

Biological Shakers Innova® 44/44R — Operating manual

8 Getting started

8.1 Platform assemblies

The Innova 44/44R can be used with a variety of Eppendorf platforms that will accept a wide range of clamps for flasks, test tubes, etc. A platform, which is required for operation, is a separate item, not included with the shaker assembly. For details on available platforms and platform accessories, (see Accessories on p. 58).

8.2 Installation of platform

In transit, there are two small plastic straps on the side of the bearing housing to secure the

Hint!

slide-out mechanism and two small plastic straps that immobilize the bearing housing; all straps must be removed.

Getting started

Hint!

Abb. 14: Install Sub platform

When you cut the bearing housing straps, be particularly careful not to cut the wiring that is in close proximity to the straps.

Prior to use, a subplatform and platform must be installed on the unit. The shaker is shipped with four Allen head platform screws installed in the bearing housing, see Figure below which also shows the subplatform that you must install (see Fig. 14 on p. 32).

Fig. 14: Install Subplatform

1 Platform Screws 2 Subplatform

3Bearing Housing

1. Remove the Allen head platform screws, setting them aside.

2. Place the subplatform onto the bearing housing, taking care to orient it as shown (see Fig. 14 on p. 32), with the notches and the lever toward the front of the shaker, lever facing up.

3. Align the subplatform holes with the tapped holes in the bearing housing, then secure the subplatform with the Allen head platform screws you previously removed.

To install the slide-out platform you have purchased:

Biological Shakers Innova® 44/44R — Operating manual

1. With reference to figure below (see Fig. 15 on p. 33), place the platform on the subplatform, making sure the two slots in the back of the platform are inserted under the subplatform’s blocks in the back, and the slide-out tray handles are at the front edge, facing up.

Steps 2 – 4 are called out in figure below (see Fig. 15 on p. 33):

Abb. 15: Install Slide-Out Platform

Getting started

Fig. 15: Install Slide-Out Platform

1 Rotate subplatform cam lever 180 °

counterclockwise (away from you), until it points to the right. This locks the platform in place.

3 Push both slide-out tray handles away from you

until the platform hits its stops at the back of the chamber.

2 Simultaneously push both side cam levers up

(away from you and toward the back of the chamber).

8.3 Install flask clamps

Flask clamps purchased for use with universal platforms (see Flask clamps for universal platforms on p. 61) require installation. Clamps are installed by securing the base of the clamp to

the platform with the correct type and number of screws. All clamps are shipped complete with hardware.

Clamps for 2, 2.8 and 4 liter flasks are shipped with an additional girdle to keep the flasks in place. The girdle is an assembly of springs and sections of rubber tubing. One girdle is already in place on the clamp, the other is packed separately. To install these double girdle clamps:

1. Place the clamp on the platform, aligning its mounting holes with holes on the platform. Secure the clamp in place using the flat Phillips head screws provided (# S2116-3051, 10 – 24 x 5/16 in). Use figure below (see Fig. 17 on p. 34) to help you identify the proper screws, as three different types of screws are shipped with the clamps.

2. With the first girdle in place, as delivered, on the upper part of the clamp body (see Fig. 16 on p. 34), insert an empty flask into the clamp.

3. After making sure the sections of tubing are located between the clamp legs, roll the first girdle down the legs of the clamp as far as it can go. The tubing sections will rest against the platform, and the springs will be under the clamp base.



Biological Shakers Innova® 44/44R — Operating manual

4. Place the second girdle around the upper portion of clamp body (just as the first girdle was initially). Make sure that its spring sections rest against the clamp legs, while its rubber tubing sections sit against the flask, in between the clamp legs.

Abb. 16: Double Girdle Clamp Installation

Fig. 16: Double Girdle Clamp Installation

Getting started

Abb. 17: Clamp Fastener

Fig. 17: Clamp Fastener

8.4 Electrical connections

1 Upper Girdle with Girdle Tubes 2 Clamp Body (Legs and Base)

3 Lower Girdle with Girdle Tubes 4 Platform

5 Clamp Mounting Holes (5)

The upper girdle secures the flask within the clamp, and the bottom girdle keeps the flask from

Hint!

spinning.

Before making electrical connections, verify that the power source voltage matches the voltage on the electrical specification plate and that the ON/OFF switch is in the OFF position. The electrical specification plate is located on the rear panel of the unit near the power connector.

Connect the power cord to the power connector, then connect the other end to a suitable, grounded receptacle. Make sure there is enough clearance to disconnect the plug whenever necessary.

Biological Shakers Innova® 44/44R — Operating manual

Abb. 18: Rear Panel

Fig. 18: Rear Panel

1 Adjustable Foot 2 Air Intake Vents (Do not block)

3Power Connector

8.5 Optional gassing manifold kit

This option is factory-installed. The manifold delivers gas into the chamber and ultimately into the flasks themselves via twelve ports. You may elect to use splitters after the manifold to increase the number of ports for your application.

8.6 Optional UV germicidal lamp

Personnel injury and equipment damage!

 Never try to operate the UV Germicidal Lamp while the shaker door is open.

WARNING !

This option, a germicidal ultraviolet lamp, is placed inside the Service Drawer, outside the chamber to help reduce the risk of contamination. The lamp is identified on the display screen as UV.

The UV germicidal lamp is factory-installed and available on refrigerated units only.

8.7 Optional photosynthetic lamps

This factory-installed option (for refrigerated units only) provides nine photosynthetic growth lamps inside the chamber. They can be turned on and off manually, by the easily set programmable timer, or by computer. These lamps are identified on the display screen as GRO. The replacement bulb part number is P0300-0221.

The recommended operating temperature when using this option is 15 °C – 37 °C.

The maximum operating temperature when using photosynthetic lamps is 70 °C.

Getting started

8.8 Optional humidity monitor

This optional factory-installed accessory allows you to monitor actual humidity levels in the chamber throughout your run. With humidity monitor, the maximum temperature is 60 °C.

8.9 Fill the water reservoir

If you choose to use the spill pan/reservoir as a water reservoir to reduce evaporation:

1. Temporarily remove the four thumbscrews (with their washers) that secure the front grille in place (Fig. 19 on p. 36).

Biological Shakers Innova® 44/44R — Operating manual

Abb. 19: Front Grille

Getting started

Fig. 19: Front Grille

8.10 Drain the water reservoir

1 Front Grille 2 Drain Hose - extends through this cutout

2. Check the drain hose to be sure it is clamped tightly.

3. Replace the grille and secure it with the four thumbscrews and the washers that you previously removed.

Never pour water directly under the subplatform. Pour water very slowly into the shallow area

Hint!

beyond the edge of the adapter plate, to protect the bearing housing.

4. Accessing the pan/reservoir from the left, the right or in front of the subplatform, slowly fill the reservoir with no more than 3 liters of distilled water.

To drain water from the water reservoir/spill pan:

1. Temporarily remove the four thumbscrews that secure the front grille (see Fig. 19 on p. 36).

2. Disengage the drain hose, direct it to a container or drain, then unclamp it, allowing the water to gravity drain.

3. Reclamp the hose, tuck it back in place, and replace the grille, securing it with the thumbscrews you previously removed.

The spill pan/water reservoir drain is located in the front, on the left, under the slide-out tray.

Hint!

Keep it clamped even if there is no water in the pan.

The drain hose should be tightly clamped when it is not being used for draining the reservoir.

Biological Shakers Innova® 44/44R — Operating manual

9 Operation

9.1 Safety precautions

Before operating the shaker, verify that anyone involved with its operation has been instructed in both general safety practices for laboratories and specific safety practices for this apparatus.

• The user is also responsible for following local guidelines for handling hazardous waste and

biohazardous materials that may be generated from the use of this equipment.

• If service should be required on a unit that is going to be returned to an Eppendorf facility, it

must be completely decontaminated and cleaned prior to its return, and a Returned Material Safety Sheet must be filled out to certify that you have complied.

• It is the responsibility of the user to carry out appropriate decontamination procedures if

hazardous material is spilled on or inside the equipment. Before using any cleaning or decontamination method other than those suggested by the manufacturer, users should check with Eppendorf that the proposed method would not damage the equipment.

Personnel injury and equipment damage!

 This equipment is not explosion-proof and should never be used with flammable substances

WARNING !

or used to grow organisms that produce flammable by-products.

Equipment damage!

 To prevent damage to the shaker and its contents, never run the shaker without a platform.

CAUTION!

9.2 Open the door

Open the door by firmly pulling the handle straight out to release the latching mechanism. You can now manually move the door up to the open position, or down to the closed position (make sure it latches closed).

9.3 Start the shaker

To initially start the shaker, close the door and turn the power switch (located on the righthand side of the control panel) to the ON position. The display will come on (first showing only New Brunswick Scientific, then briefly displaying the model number, 44 or 44R, and the stroke, 1 inch or 2 inch, and then quickly moving into the Display screen), and the audible alarm will sound. To mute it, (see Mute the audible alarm on p. 49).

When the shaker begins to operate, the LCD display will track the speed as it accelerates to the last entered setpoint. The shaking action may be started or stopped by pressing the Start/Stop button on the front panel.

The shaker will not operate if the door is open. This is indicated by the “door open” symbol

Hint!

appearing in the bottom line of the display (see Fig. 13 on p. 27).

9.4 Using the LCD screens

Operation

9.4.1 Display screen

When you turn the power on, this is the first screen to appear after the company title screen. The default display parameters are temperature (°C) and shaking speed (RPM).

You can change the displayed parameters. To replace a parameter:

1. Using the Control Knob, highlight the parameter that you wish to replace. For this example, we will replace RPM (see Fig. 20 on p. 38).

Biological Shakers Innova® 44/44R — Operating manual

ºC 23

RPM 120

DISP We 13:44

ºC 23

HRS

0.0

DISP We 13:44

Abb. 20: Chang ing Display Parameter

Fig. 20: Changing Display Parameter

2. Click the Control Knob in. RPM will flash.

3. Turn the Knob until the desired parameter appears in the highlighted field. For this example, we will select HRS.

Abb. 21: Changed Display Parameter

4. Click the Knob in to set and save the parameter (see Fig. 21 on p. 38).

Operation

Fig. 21: Changed Display Parameter

If you highlight an item, change it, but do not save your selection, after a few seconds the screen

Hint!

will revert to its previous setting.

“UV” (UV Germicidal lamp) and “GRO” (photosynthetic growth lamps) will appear in this screen with the word “NONE” if your shaker is not equipped with these optional features.

You can also use this screen to verify a setpoint, even though the values displayed here are actual (current) values. To view a setpoint:

1. Use the Control Knob to highlight the value (in this example, we will view the temperature setpoint, so we will highlight the current °C, which is 23).

2. Click the Knob in to display the current setpoint, which will flash.

At this point you can modify the setpoint or click the Knob in again to return to the normal display, which will be the actual temperature.

To modify a setpoint in this screen:

1. Use the Control Knob to highlight the current value (we will continue to use the temperature as our example, so we will select 23).

2. Click the Knob in to display the current setpoint (in this example, 38.5 (see Fig. 22 on p. 39), which will flash.

Biological Shakers Innova® 44/44R — Operating manual

ºC 38.5

RPM 120

DISP We 13:44

Abb. 22: Changing Setpoint

Fig. 22: Changing Setpoint

3. Turn or spin the Knob to reset the setpoint (in this example, turn the Knob left to decrease the setpoint to 37.0).

If you turn the Control Knob slowly, one click left or right will change the setpoint by an increment

Hint!

of one tenth of a degree Celsius (0.1 °C). If you spin the Knob quickly, the value will change by larger increments.

4. Click the Knob in to set and save this new setpoint.

Hint!

9.4.2 Summary screen

If you highlight an item, change it, but do not save your selection, after a few seconds the screen will revert to its previous setting.

5. The display will automatically return to the actual value.

To move out of this screen and into the next:

1. Use the Control Knob to highlight DISP, then click the Knob in.

DISP begins to flash.

2. Turn the Knob to the right until the next screen, Summary (SUMM), appears. If you turn too far and enter another screen, just turn the Knob back to the left to recapture the SUMM screen.

3. Click the Knob in to select the screen and to work in it.

In this screen (see Fig. 23 on p. 40), you can see both the current ACTUAL readings and the SET points for shaking speed (RPM), chamber temperature (°C), elapsed time in a programmed run (HRS) and, if you are using the optional Humidity Monitor, the percentage of relative humidity (%RH).

Operation

PARAM ACTUAL SET

RPM 100 100 ºC 45.1 45.0 HRS 0.0 0.0 %RH 50.0 N/A

SUMM Th 16:18

Biological Shakers Innova® 44/44R — Operating manual

Abb. 23: Summar y Screen

Fig. 23: Summary Screen

The current day (Su, Mo, Tu, We, Th, Fr or Sa) and time always remains visible in the lower

Hint!

Operation

righthand corner.

The only elements you can modify in this screen are setpoints. To change setpoints in this screen:

1. Turn the Knob until the desired setpoint is highlighted, then click the Knob in.

The setpoint will begin to flash.

2. Turn the Knob to the right to increase the number, or to the left to decrease it. One click left or right will increase the setpoint by an increment of one (one whole unit or one tenth unit, depending on the parameter). Move the Knob more rapidly (you can spin it) to change the value by larger increments.

3. Click the Knob in to set and save the new value.

If you highlight an item, change it, but do not save your selection, after a few seconds the screen

Hint!

9.4.3 Setup screen

will revert to its previous setting.

4. Repeat the above steps to change any or all of the other setpoints.

To move out of this screen and into the next:

1. Use the Control Knob to highlight SUMM, then click the Knob in.

SUMM begins to flash.

2. Turn the Knob to the right until the next screen, Setup (SET), appears. If you turn too far and enter another screen, just turn the Knob back to the left to recapture the SET screen.

3. Click the Knob in to select the screen and to work in it.

Here you can set the day of the week and the time (on a 24-hour clock). This screen also allows you to lock all of your settings from further changes, and to mute or enable the audible alarm.

Biological Shakers Innova® 44/44R — Operating manual

SETUP

Day : Thu Hour/Min : 16:19 Lock : Off Mute : Off

SET Th 16:19

Abb. 24: Setup Scree n

Fig. 24: Setup Screen

To change the day:

1. Turn the Knob to highlight the day (Thu in the sample screen above), then click inward once.

The day will flash.

2. Turn the knob left or right to select the day of choice: Sun, Mon, Tue, Wed, Thu, Fri or Sat.

3. Click the knob in to set and save your choice.

If you highlight an item, change it, but do not save your selection, after a few seconds the screen

Hint!

will revert to its previous setting.

To change the time (Hour/Min):

1. Turn the knob to highlight the time (16:19 in the sample screen above), then click inward once.

The time will flash.

2. Turn the knob left or right to change the time. Left moves backward, right moves forward in time. One click right or left changes by one minute; spin the knob quickly to move more rapidly.

3. Click the knob once inward to set and save your choice.

To lock the settings:

1. Turn the knob to highlight Lock, then click inward once.

The current status (Off in the sample screen above) will flash.

2. Turn the knob in either direction; the only other choice is On. Click once inward to select and save On, or continue turning to return to Off.

When you set Lock to On, a padlock icon (see Display icons on p. 29) will appear at the bottom of the screen. This icon will remain on display through all main display screens until you turn the locking function off.

To mute the audible alarm:

1. Turn the knob to highlight Mute, then click inward once.

The current status (Off in the sample screen above) will flash.

Operation

2. Turn the knob in either direction; the only other choice is On. Click once inward to select and save On, or continue turning to return to Off.

When you set Mute to On, the crossed-out speaker icon will appear at the bottom of the screen.

Hint!

This icon will remain on display through all screens until you turn the muting function off.

Biological Shakers Innova® 44/44R — Operating manual

LAMPS

Chamber : Auto UV Decont : Off Growth : Off

LAMP Th 16:20

To move out of this screen and into the next:

1. Use the control Knob to highlight SET, then click the Knob in.

SET begins to flash.

2. Turn the Knob to the right until the next screen, Lamps (LAMP), appears. If you turn too far and enter another screen, just turn the Knob back to the left to recapture the LAMP screen.

3. Click the Knob in to select the screen and to work in it.

9.4.4 Lamps screen

In this screen (see Fig. 25 on p. 42), you can turn the chamber light (Chamber), the optional UV germicidal (“decontamination”) lamp (UV Decont) and the optional photosynthetic growth lamps (Growth) on and off. On means the light is always on, and Off means the light is always off, unless you add additional programming (see Program the shaker on p. 45).

There is an additional mode for the chamber light: Auto. In Auto mode, the light will go on every time you activate the control knob or open the door. This is the default mode.

The Lamps screen, shown below, will always indicate Chamber light mode. If the shaker is not

Hint!

Abb. 25: Lamps Sc reen

equipped with the optional UV germicidal lamp and/or photosynthetic growth lamps, UV Decont and/or Growth will say None.

Operation

Fig. 25: Lamps Screen

To change the mode setting for any of the lamps:

1. Turn the Control Knob to highlight the setting for the lamp of choice, then click the knob in.

The current setting will flash (in the sample screen, we will use the Chamber light as an example.

2. Turn the Knob left or right until the desired mode setting appears (Auto in this example).

3. Click the Knob in to save the new setting.

If you highlight an item, change it, but do not save your selection, after a few seconds the screen

Hint!

will revert to its previous setting.

To move out of this screen and into the next:

1. Use the Control Knob to highlight LAMP, then click the Knob in.

LAMP begins to flash.

2. Turn the Knob to the right until the next screen, RS232 (COMM), appears. If you turn too far and enter another screen, just turn the Knob back to the left to recapture the COMM screen.

3. Click the Knob in to select the screen and to work in it.

Biological Shakers Innova® 44/44R — Operating manual

RS232

Mode : Off Baud Rate : 19200

F/W Rev : D/H

COMM Th 16:21

9.4.5 RS232 Screen

This screen (see Fig. 26 on p. 43) is used only if you have connected a personal computer to the RS-232 port (see Software interfaces on p. 31). Here you can select the RS-232 port’s Mode and

Abb. 26: RS232 Scre en

Fig. 26: RS232 Screen

the Baud Rate appropriate to your PC.

1 In this sample screen, the Display is at Revision D

and the Firmware Control Board at Revision H.

To change the Communication Mode:

1. Turn the Control Knob to highlight the current setting (Off in the sample screen above), then click the Knob in.

The current setting will flash.

2. Turn the Control Knob left or right until the desired mode setting appears (see Table below: Communication Mode):

3. Click the control knob inward once to save the new setting.

If you highlight an item, change it, but do not save your selection, after a few seconds the screen

Hint!

will revert to its previous setting.

2 Firmware Revision level (this line is for

information only)

Operation

Biological Shakers Innova® 44/44R — Operating manual

CALIBRATE

°C Offset : 0.0 Calspeed : 250

CAL Th 16:22

Tab. 4: Communication Mode

Mode Application

Off The RS-232 port is not open to communication in either direction.

Slave The shaker can be fully controlled from the computer.

Talk The shaker sends current value reports to the computer once per minute.

Monit [Monitor] The shaker responds only to “Report Requests”.

To change the Baud Rate:

1. Turn the control knob to highlight the current setting (19200 in the sample screen above), then click the knob inward once.

The current setting will flash.

2. Turn the control knob left or right until the desired setting appears: 9600, 19200 or 38400. The setting you choose should match the baud rate of your computer.

3. Click the control knob inward once to save the new setting.

To move out of this screen and into the next:

1. Use the Control Knob to highlight COMM, then click the Knob in.

COMM begins to flash.

9.4.6 Calibrate screen

Operation

Abb. 27: Calibra te Screen

Fig. 27: Calibrate Screen

1 This is a sample of the indicated RPM value.

9.4.7 Programs screen

2. Turn the Knob to the right until the next screen, Calibrate (CAL), appears. If you turn too far and enter another screen, just turn the Knob back to the left to recapture the CAL screen.

3. Click the Knob in to select the screen and to work in it.

Use this screen (see Fig. 27 on p. 44) to create a temperature offset and to calibrate the shaking speed. For details, (see Temperature offset calibration on p. 49) and (see Using Calspeed on p. 50).

Use this screen (see Fig. 28 on p. 45) to set as many as four operating programs for the shaker. Each program can have as many as 15 steps. For complete details, (see Program the shaker on p. 45).

Biological Shakers Innova® 44/44R — Operating manual

PROGRAMS

Program 1 : Off Program 2 : Off Program 3 : Off Program 4 : Off

PROG Th 16:23

PROGRAMS

Program 1 : Off Program 2 : Off Program 3 : Off Program 4 : Off

PROG Th 16:23

Abb. 28: Program s Screen

Fig. 28: Programs Screen

9.5 Program the shaker

9.5.1 Timer only

By setting an HRS setpoint in the DISP or SUMM screen, the shaker may be set to automatically stop after a preset time period of 0.1 to 99.9 hours.

If the time is set to 0.0, the shaker will operate continuously until either the door is opened or the Start/Stop button is pushed.

9.5.2 Programmed steps

The resident software for the Innova 44/44R can store up to four programs, each having as many as 15 steps. Each step can be programmed in one-minute increments, for total periods of one minute up to 99 hours 59 minutes each.

To enter the programming mode, use the Control Knob to select the PROG screen (see Fig. 29 on p. 45). At this point, you can Run a program, Edit a program, generate a New program or turn

Abb. 29: Program s Screen

Fig. 29: Programs Screen

9.5.3 Create a program

a program Off. Off is the default mode.

To write a New program:

1. Use the Control Knob to highlight the mode of Program 1 (in our sample screen, (see Fig. 29 on p. 45), it is Off), then click the Knob in.

The selected field begins to flash.

Operation

Biological Shakers Innova® 44/44R — Operating manual

PRG1 - STEP -- 01

Time 00:00 ºC 20.0 RPM Off UV Off GRO Off

 Save Cancel

2. Turn the Knob until the field says New. Click the Knob in to select this mode. The screen for

Abb. 30: Progra m 1, Step 1

Program 1 - Step 1 will open (see Fig. 30 on p. 46):

Fig. 30: Program 1, Step 1

1 Step Number 2 Use to leave programming mode without saving

3 Do not use until programming is complete. 4 Use these arrows to scroll through steps.

Operation

If your shaker is not equipped with these optional features, “UV” and “GRO” will appear with the

Hint!

word “Off” in this screen but will not be programmable.

3. Turn the Knob to highlight the Time setting (00:00 in the sample screen above), then click the Knob in. The field will flash.

4. Turn the Knob until the desired running duration for this step (from 00:01, which means one minute, to 99:59) appears, then click the Knob in to save the setting. We will set Step 1 time to eight hours for this example (see Fig. 31 on p. 47).

5. Turn the Knob to highlight the °C temperature setting (20.0 in the sample screen above), then click the Knob in. The field will flash.

6. To set the temperature desired (°C from 4.0 to 80.0) for the time period you have set, turn the Knob (left to decrease, right to increase). When the desired value appears, click the Knob in to save the setting. We will set Step 1 temperature to 37.0 °C (see Fig. 31 on p. 47).

7. Turn the Knob to highlight the RPM setting (Off in the sample screen above), then click the Knob in. The field will flash.

8. Turn the Knob to select the desired shaking speed (25 to 400 RPM) for this time period, then click the Knob in to save the setting. We will set Step 1 speed to 150 RPM (see Fig. 31 on p. 47).

9. If you do not have the optional UV lamp, skip this step. If you have the UV lamp (default setting is Off) and you wish to have it on during the time period you have set, use the Control Knob to select the field, click the Knob to select the field (it will flash), turn the Knob to select On, then click the Knob in to save the setting. In our sample Step 1 (see Fig. 31 on p. 47), however, UV remains Off.

10. If you do not have the optional GRO lamps, skip this step. If you have the photosynthetic lamps (default setting is Off) and you wish to have them on during the time period you have

any new settings.

Biological Shakers Innova® 44/44R — Operating manual

set, use the Control Knob to select the field, click the Knob to select the field (it will flash), turn the Knob to select On, then click the Knob in to save the setting. In our sample Step 1 (see Fig. 31 on p. 47), however, GRO remains Off.

DO NOT select “Save” yet!

Hint!

11. To program Step 2 (see Fig. 31 on p. 47): use the Control Knob to highlight the arrows at the bottom left of the screen. Click the Knob to make the arrows flash, then turn the Knob to the right until Step 2 appears. Click the Knob in to work in this screen, and repeat Steps 3 – 10. Whatever step is shown on the right-hand side of the display is settable ((see Fig. 31 on p. 47), it is Step 2). To scroll between steps, select the arrows (bottom left), then turn the

Abb. 31: Progra m 1, Step 2

Control Knob clockwise or counterclockwise, then select the desired step.

PRG1 - STEP 01 02

Time 08:00 03:00 ºC 37.0 40.0 RPM 150 225 UV Off Off GRO Off Off

 Save Cancel

Fig. 31: Program 1, Step 2

1Step Number

The time entered for each step is for that step only; it is not cumulative (i.e. the elapsed time from

Hint!

Abb. 32: Progra m 1, Step 3

the start of the program).

12. Continue to program as many as 15 steps in the same manner. For our sample program, we

Operation

have only three steps (see Fig. 32 on p. 47).

PRG1 - STEP 02 03

Time 03:00 12:00 ºC 40.0 4.0 RPM 225 0 UV Off Off GRO Off Off

 Save Cancel

Fig. 32: Program 1, Step 3

1Step Number

PROGRAMS

Program 1 : Off Program 2 : Run Program 3 : Off Program 4 : Off

PROG Fr 08:31

Biological Shakers Innova® 44/44R — Operating manual

The three-step program we have illustrated is designed to start incubating cultures at 37 °C and hold that temperature for eight hours, shaking at 150 RPM. After eight hours, Step 2 engages, increasing the temperature setpoint to 40 °C for a temperature induction, holding that temperature for three hours and increasing the shaking speed to 225 RPM. After this interval, the temperature will be cooled to 4 °C and held at that temperature for twelve hours; no shaking will occur during this period, as the speed is set to 0 RPM.

Please note that temperature reductions, even at temperatures above ambient (going from 40 °C to 30 °C, for example), require refrigeration to be effective.

When the last step of a program is completed, the Innova 44/44R will continue operating

Hint!

9.5.4 Edit a program

according to the parameters of the final step programmed, until the user intervenes.

If you wish the shaker contents to experience different conditions (e.g., a different shaking speed or no shaking at all, and/or return to ambient temperature), set those parameters as the last step in your program.

To save the entire program:

1. Once all your steps are set, use the Control Knob to highlight Save at the bottom of the screen, then click the Knob in. The field will flash.

2. Click the Knob in again to save the program. The display will read Process Running – Saving Profile for a few seconds, then return to the main Programs (PROG) screen.

If you wish, you can set Programs 2, 3 and 4 and save them in the same manner.

Use the Edit function to open a program you have already created and saved, in order to modify your settings following the same procedures.

9.5.5 Run a program

Use the Run fuction to turn a specific program on. Naturally, only one program can run at a time.

Operation

Abb. 33: Program s Screen, Running

When you change the mode to Run, the screen will show the Run icon:

Fig. 33: Programs Screen, Running

1Run Icon

Biological Shakers Innova® 44/44R — Operating manual

To stop a program: You can abort a run any time by changing the program’s function to Off.

9.6 Mute the audible alarm

The Innova 44/44R shakers have an audible alarm that is activated under predetermined conditions. It may be muted in the following way:

1. Turn the Control Knob until the SET screen is highlighted on the display. Click the Knob in to work in this screen.

2. Turn the Knob to highlight the Mute mode (On), then click the Knob in. The field will flash.

3. Turn the Knob to change the setting to Off, then click the Knob to save this selection.

To reactivate the audible alarm at any time, repeat steps 1 – 3, reversing “off” and “on”.

9.7 Temperature offset calibration

The temperature probe and the temperature controller are calibrated together at the factory. The temperature probe measures the temperature of the air at the probe’s location, near the return vent. The controller uses the probe input to adjust air temperature, up or down, to match the temperature setpoint.

Depending on various conditions within the chamber, such as flask placement and size, the heat produced by growing organisms, heat losses due to liquid evaporation from flasks, etc., the display temperature may differ from temperatures within the flasks themselves. You can calculate the correction value for this offset and program the shaker to display a corrected temperature.

9.7.1 Calculate the offset value

If you wish to have the temperature display (“Indicated Temperature”) match the temperature at a given point, or match the average of a series of points within the chamber (“Actual Temperature”), proceed as follows:

1. Let the unit equilibrate at or near the desired temperature, then record the Indicated Temperature.

2. Now record the Actual Temperature.

3. Calculate the temperature correction value using this formula: Actual Temperature – Indicated Temperature = Temperature Offset Value.

4. To set the Temperature Calibration Offset, follow the procedure outlined in the next paragraph below.

9.7.2 Set the offset

To set the temperature calibration Offset:

1. Use the Control Knob to enter the CAL screen (see Fig. 34 on p. 50).

2. Turn the Control Knob to highlight the current setting (0.0 in the sample screen above), then click the Knob in. The current setting will flash.

3. Turn the Knob (left for negative settings or right for positive settings) to display the desired setting. As you turn the Knob, each click represents one tenth of a degree Celsius (0.1 ºC).

4. When you reach the desired setting, click the Control Knob in to save the new value.

5. When the Temperature Offset is set to any value other than 0, the asterisk icon will appear next to ºC in the DISP and SUMM screens.

Operation

If you highlight an item, change it, but do not save your selection, after a few seconds the screen

Hint!

will revert to its previous setting.

CALIBRATE

°C Offset : 0.0 Calspeed : 250

CAL Th 16:22

Biological Shakers Innova® 44/44R — Operating manual

Abb. 34: Calibra te Screen

Fig. 34: Calibrate Screen

1 This is a sample of the indicated RPM value.

9.8 Using Calspeed

The Calspeed function, which is set in the CAL screen (see Fig. 34 on p. 50), is used to calibrate the speed of the shaking mechanism. Calibrated at the factory, speed does not need to be recalibrated until a major operating component (e.g. drive belt) is changed.

Prior to engaging the Calspeed function, make sure that the platform is properly secured to the

Hint!

Operation

9.9 Power interruption

9.10 Slide-out platform

subplatform, and any flasks present are secured. Run the unit at approximately 250 RPM to ensure stable operation prior to calibration.

When the shaker is running, the CAL screen shows the indicated RPM value. If you wish to calibrate the speed, set the speed to a value that can be measured, a setpoint of 250 RPM works well. The use of a strobe is recommended for accuracy. If, after measuring the actual speed, you wish to adjust the indicated value:

1. Click the Control Knob in.

2. Set the new value.

3. Click the Knob in again to save the setting.

4. Turn the shaker OFF, wait a few seconds, then turn the shaker back ON.

In the event of a power failure, the Innova 44/44R Shakers are equipped with an automatic restart function. The shaker’s non-volatile memory retains all stored information.

If the shaker was in operation prior to the power interruption, the shaker will begin to operate at its last entered setpoints. The alarm/POWER display will flash, indicating that a power interruption has occurred. Turn the Control Knob in any direction to acknowledge the visual alarm. The flashing will stop.

The Innova 44/44R is equipped with a slide out platform mechanism as a standard feature. This allows you to easily slide the platform out of the shaker for easy access to the platform without the need for tools.

To operate the platform slide-out mechanism:

1. Make sure platform has stopped moving.

2. Rotate cam lever located on the subplatform (see Fig. 35 on p. 51) 180 ° clockwise. It should be pointing to the left.

Biological Shakers Innova® 44/44R — Operating manual

3. Pull both side levers (see Fig. 35 on p. 51) down. This will disengage the platform from the subplatform.

4. Pull both handles on the slide-out mechanism (see Fig. 35 on p. 51) toward you. This will

Abb. 35: Disengag ing Slide-Out Platform

move the platform to an easily accessible position.

Fig. 35: Disengaging Slide-Out Platform

1 Rotate subplatform cam lever 180 ° clockwise

(toward you), until it points to the left.

3 Pull both slide-out tray handles toward you.

5. To re-insert the platform, reverse this process. Make sure the two slots in the back of the platform are inserted under blocks of subplatform in the back.

Make sure that you have rotated the front cam lever counter-clockwise to lock the platform in

Hint!

position.

Operation

2 Pull both side levers down (toward you)

simultaneously.

Biological Shakers Innova® 44/44R — Operating manual

10 Troubleshooting

10.1 General troubleshooting

Troubleshooting

If any problems occur with your shaker, do not attempt to perform any service on the unit. Unauthorized servicing may void the warranty. Please contact your local Eppendorf Customer Service Department.

In any correspondence with Eppendorf, please refer to the model number and serial number of your unit. This information is on the electrical specification plate, located on the rear panel of the unit, above the power connector. The serial number is also located in the lower right corner panel, below the chamber door seal.

There are some problems, however, that you can investigate and correct yourself. Refer to the following Troubleshooting Guide:

Symptoms Probable Causes and Solutions

No power; display is not on; power cord is not plugged in and/or power switch is off: plug in power cord (to working electric outlet), and turn on power switch.

Door is open—look for Open Door icon on display: close door firmly, making sure latch is engaged.

Door is closed but Open Door icon is on display: door magnet is not adjusted correctly; call for service.

On/Off switch is not working: call for service.

Shaker does not run.

Shaker runs slowly and/or no

speed indication.

Tilt Switch has been triggered. Alarm condition Tilt will be indicated and RPM will be OFF. Check to ensure the load is evenly distributed and that the shaker is level on a solid surface. Restart by pressing the START/STOP button.

Fuse(s) burned out: check and replace as needed.

If you recently opened the Service Drawer to replace a fuse, it may not have been seated properly: reopen and reclose carefully.

Shaking speed has been set to Zero by program running (look for Run icon in display) or by computer interface: reset shaking speed.

Defective main board: call for service

Defective display controller board: call for service.

Jammed shaking mechanism: call for service

Defective motor: call for service

Drive belt out of alignment or worn: call for service.

If you recently opened the Service Drawer to replace a fuse, it may not have been seated properly: reopen and reclose carefully.

Fuse(s) burned out: check and replace as needed.

Incorrect speed calibration: recalibrate shaking speed.

Defective main board: call for service.

Defective motor: call for service.

Drive belt out of alignment or worn: call for service.

Biological Shakers Innova® 44/44R — Operating manual

Shaker is running in Program mode; Look for Run icon in display.

Shaker speed has been changed by RS-232 command/ computer interface.

Shaker does not run at set speed.

Operating noise

Shaker is overloaded: remove some contents and balance load.

Defective motor: call for service.

Drive belt out of alignment or worn: call for service.

Load out of balance: pull out Slide-Out Platform, unload all contents, reload.

Loose component(s) in Slide-Out Platform, subplatform, and/or drive assembly: call for service.

Shaker running in Program mode: Look for Run icon in display.

Temperature setpoint changed by RS-232/computer interface command.

Heater fuse blown: replace.

Compressor fuse blown: replace.

Compressor over-pressure switch activated: call for service.

Chamber fans not working: call for service

Ambient temperature too high or too low: cool or heat the room as needed.

Troubleshooting

Incubator does not reach set

temperature.

Incorrect temperature indication.

Incorrect relative humidity % Defective humidity probe or broken contact: call for service.

Door is not completely closed (even though Open Door icon may not be on display): open it and reclose it firmly.

Sealing gasket between the Service Drawer and the Inner Cabinet is damaged: call for service to replace it.

Line voltage is too low.

Frequency on line voltage if set incorrectly: reset.

Firmware revision does not match the Service Drawer revision: ask for update.

Defective heater: call for service.

Defective refrigeration system: call for service.

Incorrect temperature indication (see below).

Temperature Offset has been programmed; Look for * adjacent to C on display.

Defective RTD assembly: call for service.

Defective main board: call for service.

Biological Shakers Innova® 44/44R — Operating manual

Photosynthetic GRO lamp does

Troubleshooting

UV germicidal lamp does not work.

Shaker is running in Program mode; Look for Run icon in display and program calls for GRO lamp to be off.

GRO lamp mode has been changed by RS-232 command/ computer interface.

Bulb is burned out; replace

Fuse is burned out; replace

not work.

Check ballast voltage.

Check cable connections.

Check switch settings.

GRO lamp mode has been changed by RS-232 command/computer interface.

Shaker is running in Program mode; Look for Run icon in display, and program calls for UV lamp to be off.

UV lamp is burned out; replace

Fuse is burned out; replace

Check ballast voltage.

Check cable connections.

Check switch settings.

UV lamp mode has been changed by RS-232 command/ computer interface.

Door does not stay in the fully open

position.

Adjust the door tension

Biological Shakers Innova® 44/44R — Operating manual

11 Maintenance

11.1 Routine maintenance

No routine maintenance schedule is required for the Innova 44 and 44R.

Personnel injury and equipment damage!

 When cleaning the unit, always turn off the shaker and disconnect the power cord from the

power supply.

WARNING !

To ensure that your shaker retains its attractive appearance, an occasional cleaning, using a cloth with conventional household (non-abrasive) cleaner is recommended (see Cleaning external and internal surfaces on p. 55).

We recommend you occasionlly wipe down the shaker with a non-abrasive household cleaner.

We also suggest that the area around the shaker be vacuumed or swept to remove dust and other debris, ensuring proper air flow in and around the shaker.

11.2 Cleaning external and internal surfaces

Personnel injury and equipment damage!

 When cleaning the unit, always turn off the shaker and disconnect the power cord from the

power supply.

WARNING !

The unit may be cleaned using a damp cloth or any standard household or laboratory cleaner to wipe down its outer surfaces. Do not use abrasive or corrosive compounds to clean this instrument, as they may damage the unit.

11.3 Biohazard decontamination

Maintenance

WARNING !

It is the responsibility of the user to carry out appropriate decontamination procedures if hazardous material is spilled on or inside the equipment. Before using any cleaning or decontamination method other than those suggested by the manufacturer, users should check with Eppendorf that the proposed method would not damage the equipment.

Commercially available household bleach solutions, when diluted at a 1:10 ratio, are effective in routine decontamination of the instrument. The method for decontaminating a spill depends upon the nature of the spill.

Spills involving fresh cultures or samples known to have low concentrations of biomass should be flooded with decontamination solution and soaked for 5 min before cleanup. Spills involving samples with high concentrations of biomass, or involving organic matter, or occurring in areas warmer than room ambient temperature, should be exposed to decontamination solution for at least one hour before cleanup.

Personnel injury!

 Personnel involved in the cleanup of any spill should wear gloves, safety glasses, and a

laboratory coat or gown during the cleanup process. Respiratory protection should be considered for spills where aerosolization is suspected.

Biological Shakers Innova® 44/44R — Operating manual

12 Technical data

12.1 Specifications

These specifications assume a maximum load of 15.5 kg (34 lb), including platform, clamps, glassware and contents.

Innova 44 and 44R Incubator Shaker

Technical data

SHAKING

TEMPERATURE

Speed

Control Accuracy ±1 rpm

Indication Displayed in 1 rpm increments

Stroke/Orbit 2.5 cm (1 in) or 5.1 cm (2 in)

Range (44)

Range (44R)

Control Accuracy ±0.1 °C from 30 – 40 °C, ±0.5 °C over the remaining range.

Indication Displayed in 0.1 °C increments

Heater

ALARMS

LCD DISPLAY

RS-232

SETPOINT

RETENTION

AUTOMATIC RESTART

25 – 400 rpm with 2.5 cm (1 in) orbit or 25 – 300 rpm with 5.1 cm (2 in) orbit or with 2 units stacked; 25 – 250 rpm for 3 units stacked

5 °C above ambient temperature to 80 °C

20 °C below ambient temperature (minimum 4 °C) to 80 °C

Long-life, low-watt density resistance-type heater with high temperature thermostat

Visible and audible warning indication when speed deviates more than 5 rpm or temperature more than 1 °C from setpoints, and when timer has expired. Audible alarm can be muted.

240 x 128 backlit

Remote control, remote monitoring, remote data logging

All setpoints and operating status are retained in non-volatile memory

Automatic restart after power is restored, indicated by flashing display

STACKING

DRIVE

DRIVE MOTOR

SAFETY

ELECTRICAL

REQUIREMENTS

ETL REGULATORY

STANDARDS

Up to three units may be stacked; the second and third units require stacking kit(s).

Triple-eccentric counterbalanced drive with nine permanently lubricated ball bearings

Solid-state brushless DC motor.

Drive Interrupt shuts off power to Shaker when door opens. Acceleration/deceleration circuit prevents sudden starts and stops, minimizing both splashing and mechanical damage. Independent mechanical sensing switch also shuts the motor off in unbalanced condition.

100 V, 50Hz

100 V, 60 Hz

120 V, 60 Hz

230 V, 50 Hz

UL 61010A-1, CAN/CSA-C22.2 No. 1010.1

UL 61010A-2-010, CAN/CSA-C22.2 No. 1010.2.010

44: 800 VA per shaker

44R: 1500 VA per shaker

Biological Shakers Innova® 44/44R — Operating manual

DIMENSIONS

GROSS WEIGHT

CE REGULATORY

STANDARDS

Width 135 cm (53 in) 135 cm (53 in) 135 cm (53 in)

Depth (Front to Back) 84 cm (33 in) 84 cm (33 in) 84 cm (33 in)

Height 66 cm (26 in) 132 cm (52 in) 198 cm (78 in)

CHAMBER

DIMENSIONS

CHAMBER DOOR

PLATFORM

44 238 kg (525 lb)

44R 259 kg (570 lb)

Short Base 16.4 kg (36 lb)

Medium Base 40.9 kg (90 lb)

Tall Base 57.3 kg (126 lb)

Altitude Limit 2000 Meters

See Appendix B

Single Unit Two Units Three Units

66 cm deep X 91 cm wide X 46 cm high from top of platform

(26 in deep X 36 in wide X 18 in high from top of platform)

Door glides out and up.

Aluminum, 46 X 76 cm (18 X 30 in). Select universal or dedicated styles PLEASE NOTE: Innova 44 and 44R platforms are NOT interchangeable with platforms from other New Brunswick shakers.

Technical data

Use of baffled flasks will significantly reduce maximum speed for any shaker.

depending on ambient factors, such as relative humidity, and options installed

Add 53 cm (21 in) to height and 15.2 cm (6 in) to depth for door clearance.

Biological Shakers Innova® 44/44R — Operating manual

13 Ordering information

13.1 Replacement parts

In the table below you will find commonly needed replacement parts for your Innova 44/44R shaker:

Part Description Quantity Part No.

Spare Parts Kit (includes 1 motor drive poly-V belt, and two each 10 A, 6.3 A and 3.15 A fuses)

Flask Clamp screws, 10 – 24 x 5/16 in flat Phillips head (+) 25 per package S2116-3051P

Test Tube Rack screws, 10 – 24 x 1/2 in pan head, and washers (#10 plain and #10 lockwashers)

Motor Drive Poly-V Belt, 28 inches long 1 P0700-7070

Fuse, 10 A 1 P0380-3162

Fuse, 8.0 A 1 P0380-3790

Fuse, 3.15 A 1 P0380-3460

Chamber Light Bulbs 2 P0300-0470

UV Germicidal Bulb (if so equipped) 1 P0300-0351

Photosynthetic Bulb (if so equipped) 1 P0300-0221

UV Germicidal Light Ballast (if so equipped) 1 P0420-5113

Photosynthetic Light Ballast (if so equipped) 1 P0420-5192

13.2 Accessories

Ordering information

1 M1282-6000

10 each M1289-0800

When ordering accessories, you may be asked to provide the model number and serial number of your shaker. This information is on the electrical specification plate, located on the rear panel of the unit.

The serial number is also labeled in the lower right corner of the front panel, below the chamber door seal.

13.2.1 Slide-out platforms

The Slide-Out Platform allows you to extend the platform in front of the shaker for easy access to all flasks. Here are the various platforms available:

Description Part No.

Universal Platform M1282-9904

125 mL Erlenmeyer flask Dedicated Platform M1282-9905

250 mL Erlenmeyer flask Dedicated Platform M1282-9906

500 mL Erlenmeyer flask Dedicated Platform M1282-9907

1 L Erlenmeyer flask Dedicated Platform M1282-9908

2 L Erlenmeyer flask Dedicated Platform M1282-9909

2.8 L Fernbach flask Dedicated Platform M1282-9910

4 L Erlenmeyer flask Dedicated Platform M1282-9911

Should you decide in favor of the Universal Platform, following is a list of that particular platform’s flask capacity, according to flask size:

Biological Shakers Innova® 44/44R — Operating manual

Flask Type

50 ml Erlenmeyer Flasks 91

125 ml Erlenmeyer Flasks 39

250 ml Erlenmeyer Flasks 30

500 ml Erlenmeyer Flasks 24

1 L Erlenmeyer Flasks 14

2 L Erlenmeyer Flasks 8

2.8 L Erlenmeyer Flasks 6

4 L Erlenmeyer Flasks 6

5 L Erlenmeyer Flasks 6

Clamps for Universal Platform are sold separately.

Capacity

Ordering information

Biological Shakers Innova® 44/44R — Operating manual

Tab. 5: Platform Capacity Chart

Innova Model 44/44R

Platform

(in and cm)

Dedicated Platform (D)

Universal Platform (U)

10 mL – 187

25 mL – 93

50 mL – 91

125 mL 60 39

250 mL 40 30

500 mL 24 24

1 L 15 14

2 L 12 8

2.8 L

4 L

5 L –

30 in x 18 in

76 cm x 46 cm

1,2

Ordering information

Microplate Rack (1 layer) – 4

6 L – –

Large TT Rack – 7

Medium TT Rack – 9

Small TT Rack – 9

Microplate Rack (stack) – 16

Maximum flask size when using Photosynthetic Light Bank is 4 Liters

Using Sticky Tape - less capacity with clamps

Biological Shakers Innova® 44/44R — Operating manual

13.2.2 Flask clamps for universal platforms

The following clamps, according to flask size, are available for use with the Universal Platform:

Clamp Size Part No.

10 ml Erlenmeyer Flask ACE-10S

25 ml Erlenmeyer Flask M1190-9004

50 ml Erlenmeyer Flask M1190-9000

125 ml Erlenmeyer Flask M1190-9001

250 ml Erlenmeyer Flask M1190-9002

500 ml Erlenmeyer Flask M1190-9003

1 L Erlenmeyer Flask ACE-1000S

2 L Erlenmeyer Flask ACE-2000S

2.8 L Fernbach Flask ACSB-2800S

4 L Erlenmeyer Flask ACE-4000S

5 L Erlenmeyer Flask ACE-5000S

13.2.3 Replacement clamp hardware kits

Eppendorf flask clamps come complete with mounting screws. Additional screws are available separately in packs of 25 (Part number S2116-3051P).

13.2.4 Test tube racks and other accessories

Accessory Description Part Number Platform Capacity

Adjustable angle Test Tube Rack for tubes 8mm – 11mm diameter

Adjustable angle Test Tube Rack for tubes 12mm – 15mm diameter

Adjustable angle Test Tube Rack for tubes 15mm – 18mm diameter

80 tube capacity M1289-0100 7

60 tube capacity M1289-0010 9

48 tube capacity M1289-0001 9

60 tube capacity M1289-0200 7

44 tube capacity M1289-0020 9

34 tube capacity M1289-0002 9

42 tube capacity M1289-0300 7

31 tube capacity M1289-0030 9

24 tube capacity M1289-0003 9

Ordering information

Adjustable angle Test Tube Rack for tubes 18mm – 21mm diameter

30 tube capacity M1289-0400 7

23 tube capacity M1289-0040 9

18 tube capacity M1289-0004 9

Biological Shakers Innova® 44/44R — Operating manual

Adjustable angle Test Tube Rack for tubes 22mm – 26mm diameter

Adjustable angle Test Tube Rack for tubes 26mm – 30mm diameter

Microplate holder rack (stacked)

Microplate holder rack (single layer)

Angled Test Tube Rack Holder racks that are 10mm – 13mm (4in – 5in) wide and up to 38mm (15in) long.

Angled Test Tube Rack Spacer Bar to accommodate test tubes racks that are less than 13mm (5in) wide.

13.2.5 Optional bases

22 tube capacity M1289-0500 7

16 tube capacity M1289-0050 9

13 tube capacity M1289-0005 9

20 tube capacity M1289-0600 7

16 tube capacity M1289-0060 9

12 tube capacity M1289-0006 9

3 deep well or 9 standard M1289-0700 16

5 deep well or standard TTR-221 4

for user-supplied test tube

for use with TTR-210

Universal Platform Required

TTR-210 4

TTR-215 NA

Short Base, raises bottom unit 10 cm (4 in) M1282-0600

Medium Base, raises bottom unit 30.5 cm (12 in) M1282-0800

Ordering information

Tall Base, raises bottom unit 40.6 cm (16 in) M1282-0700

13.2.6 Optional stacking kit

Stacking hardware (to stack two Innova 44 shakers) M1282-0500

Stacking hardware (to stack an Innova 4400 on an Innova

44)

13.2.7 Optional handles

Screw-In Handles (6 pieces in kit) M1282-5042

Description Part Number

M1282-0501

Description Part Number

Biological Shakers Innova® 44/44R — Operating manual

14 Transport, storage and disposal

14.1 Transport and storage

When transporting or storing the device, always use the original packaging material.

14.2 Disposal

In case the product is to be disposed of, the relevant legal regulations are to be observed.

Information on the disposal of electrical and electronic devices in the European Community:

Within the European Community, the disposal of electrical devices is regulated by national regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic equipment (WEEE).

According to these regulations, any devices supplied after August 13, 2005, in the business-to-business sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste. To document this, they have been marked with the following identification:

Because disposal regulations may differ from one country to another within the EU, please contact your supplier if necessary.

In Germany, this is mandatory from March 23, 2006. From this date, the manufacturer has to offer a suitable method of return for all devices supplied after August 13, 2005. For all devices supplied before August 13, 2005, the last user is responsible for the correct disposal.

Transport, storage and disposal

Biological Shakers Innova® 44/44R — Operating manual

15 Appendix A: Remote programming

15.1 Setting up with HyperTerminal

Hint!

Appendix A: Remote programming

HyperTerminal is only present in Microsoft® Windows XP and older.

You can use the Microsoft HyperTerminal program on your personal computer to communicate with your shaker:

1. Before you set up HyperTerminal, be sure to check the Mode and Baud Rate parameter settings (see RS232 Screen on p. 43).

2. While you are in the RS232 screen (see Fig. 26 on p. 43), set the Mode parameter to Slave, and select the appropriate Baud Rate. The default setting, as you see in your screen, is 19200; your other choices are 9600 or 38400.

3. Connect the RS-232 cable between your computer and the shaker’s RS-232 port (see Fig. 12 on p. 26).

4. Start HyperTerminal, and follow the steps for Setting a New Connection. When prompted, select the COM port you will be using, then set the COM parameters (see table below).

5. To be sure that you have established a connection with your shaker, turn the shaker OFF, open the HyperTerminal connection, then turn the shaker back ON. Once a connection has been made, the shaker will send an OK to HyperTerminal.

6. You may wish to send a trial command to the shaker (see Sample Test below). We suggest that you enable Caps Lock on your computer, because all commands must be sent as capital letters.

Biological Shakers Innova® 44/44R — Operating manual

Tab. 6: COM Parameters for HyperTerminal

Baud Rate Enter the setting on your shaker (see Step 2 above)

Data Bits 8

Parity None

Stop Bits 1

Flow Control None

Sample Test:

1. To request the current setpoint, type RS at the terminal. When you type R, you should see the R on the computer display. This is an echo character, sent back by the Innova 44/44R. When

you type the S, you should see the echo S displayed at the computer.

2. Use the Enter key to send the carriage return control character. This will move the cursor back over the first character.

3. RS is a Request command, so your shaker will send back the data corresponding to the command.

15.2 Overview of command sets

Using an optional RS-232 interface, the user has the capability to control various functions of the Innova 44/44R shakers by computer. A detailed command set follows this introduction.

The serial interface uses an echo character protocol setting that is implemented to indicate that a valid character has been received by the shaker. For every character sent by the user computer, the Innova 44/44R will return a matching character. This echo character should be read by the computer and compared to the character that was sent.

Appendix A: Remote programming

When you are sending a command, do not allow the pause between any two characters of your

Hint!

CS<Space>150<CR> CS<Space>150<CR>

RV<CR> First the echo: RV<CR>

Hint!

message to last longer than 10 seconds. If it takes any longer, the shaker’s serial port will reset itself.

In the following example, the Master (your computer) will command (the C in CS) the shaker (Slave) to set its speed to 150 RPM:

Master sends: Innova 44/44R responds:

In the next example, the Master (your computer) will request that the shaker (Slave) report (the R in RV) the actual parameters:

Master sends: Innova 44/44R responds:

Then the report:

On power-up, even if the shaker is not set in Slave mode at the time, the shaker will always send these four characters: OK<CR><LF>. Before starting any communications, be sure to clear the computer’s receive buffer.

This is the command set format:

Most commands are defined as two capital characters using standard ASCII format (see the command sets provided below).

Biological Shakers Innova® 44/44R — Operating manual

If a command has additional arguments, the ASCII space control character separates each argument. The command is terminated using the ASCII carriage return control character; an optional line feed control character may also be included but must follow the carriage return character.

When a command is sent that returns data, the characters returned are in ASCII format. Each additional argument returned will be separated by the ASCII space or tab control character; refer to the selected command for the actual format. Data returned is terminated with a carriage return line feed control character sequence.

The next section provides an Index to the 2 character command codes.

The next four sections provide command sets for your use for Set Commands, Profile Control Commands, Report Request Commands and Set/Get Date & Time.

15.3 Index to command codes

Command Code Meaning

CL Command Grow Lamp

CS Command Speed

Appendix A: Remote programming

CT Command Temperature

CU Command UV Lamp

PC Clear Profile

PM Profile Mode

PR Read Profile Step in Memory

PS Profile Stop/Start

PW Write Profile Step to Memory

RI Report Software Version

RP Report Parameter List

RS Report Setpoint Values

RV Report Measured Values

=D Set Date and Time

?D Get Date and Time

15.4 Set commands

Code Meaning Format

Command Grow Lamp CL<Space><Arg1><CR> where Arg1=Set Grow

Lamp state (0 = off, 1 = on)

Command Speed CS<Space><Arg1><CR> where Arg1= Agitation

setpoint (####)

Command Temperature CT<Space><Arg1><CR> where Arg1= Temperature

setpoint (###.#)

Command UV Lamp CU<Space><Arg1><CR> where Arg1= Set UV Lamp

state (0 = off, 1 = on)

Biological Shakers Innova® 44/44R — Operating manual

15.5 Profile control commands

Code Meaning Format

PC Clear Profile (clears profile

step)

PM Profile Mode (returns status of

current profile run/hold mode)

PR Read Profile step in memory PR<Space><Arg1><Space><Arg2><CR> where Arg1=

PS Profile Stop/Start (stops or

starts a program profile)

PW Write Profile step to memory PW<Space><Arg1><Space><Arg2><Space><Arg3><Spac

PC<Space><Arg1><Space><Arg2><CR>

where Arg1= Profile number (range 1 to 4) and Arg2=Step number (range 1 to 15)

NOTE: If Arg2 is not included, all 15 steps of the selected profile will be cleared.

PM<CR> There are no parameters to this command.

Return data format: <Arg1><Space><Arg2><Space><Arg3><CR>

where Arg1=Run/hold status (0 = end, 1 = run), Arg2=Profile number and Arg3=Step number

NOTE: If a profile has not been selected to run, a 0 will be returned for Profile number and Step number.

Profile number (range 1 to 4) and Arg2=Step number (range 1 to 15)

Return data format: <Arg1><Space><Arg2><Space><Arg3><Space>...<CR><L F>

where Arg1=Profile number (range 1 to 4), Arg2=Step number, Arg3=Temperature setpoint, Arg4=Agitation setpoint, Arg5=CO2 setpoint (future option, reads 0.0), Arg6=Step hours, Arg7=Step minutes, Arg8=UV lamp, and Arg9=Grow lamp

PS<CR> With no other parameters, any profile currently running will be stopped.

PS<Space><Arg1><Space><Arg2><CR>

where Arg1=Profile number (range 1 to 4), and Arg2=Step number (range 1 to 15).

NOTE: If Arg2 is not included, the profile will start in Step 1.

e>...<CR>

where Arg1=Profile number (range 1 to 4), Arg2=Step number (range 1 to 15), Arg3=Temperature setpoint, Arg4=Agitation setpoint, Arg5=CO2 setpoint (future option); Enter 0.0, Arg6=Step hours (range 0 to 99), Arg7=Step minutes (range 0 to 59), Arg8=UV lamp (0 = off, 1 = on), and Arg9=Grow lamp (0 = off, 1 = on)

Appendix A: Remote programming

For example, the following PW command:

PW<Space>25.0<Space>150<Space>0.0<Space>2<Space>30<Space>0<Space>1<CR>

will set Program 1, Step 1 to the following conditions:

• Temperature setpoint 25.0 °C

• Agitation setpoint 150 rpm

• CO2 setpoint 0.0 %

• Step Time 2 hours 30 minutes

• UV Lamp OFF

• GRO Lamp ON

Biological Shakers Innova® 44/44R — Operating manual

15.6 Report request commands

Code Meaning Format

RI Report Software Version RI<CR>

Return data format: <Arg1><Tab><Arg2><CR><LF>

where Arg1=Display module software version, and Arg2=Control module software version

RP Report Parameter List (ASCII

text header)

RV Report Measured Values RV<CR>

Appendix A: Remote programming

RS Report Setpoint Values

15.7 Set/Get date and time

Code Meaning Format

=D Set Date and Time =D<Space><Arg1><Space><Arg2><Space><Arg3><Spac

?D Get Date and Time

RP<CR>

Return data format: <Arg1><Tab><Arg2><Tab><Arg3>...<Arg6><CR><LF>

where Arg1=”Shaker Speed”, Arg2=”Temperature”, Arg3=”% Relative Humidity”, Arg4=”% CO2 Concentration”, Arg5=”Grow Lamp Status”, and Arg6=”UV Lamp Status”

RS<CR>

Return data format: <Arg1><Tab><Arg2><Tab><Arg3>...<Arg6><CR><LF>

where Arg1= Shaker Speed, Arg2=Temperature, Arg3=% Relative Humidity, Arg4=% CO2 Concentration (future option, reads 0.0), Arg5=Grow Lamp Status, and Arg6=UV Lamp Status

e>...<Arg7><CR>

?D<CR>

Return data format: <Arg1><Tab><Arg2><Tab><Arg3><Tab>...<Arg7><CR><L F>

where Arg1=Hours (range 0-23), Arg2=Minutes (range 0-59), Arg3=Seconds (range 0-59), Arg4=Year (range 00-99), Arg5=Month (range 01-12), Arg6=Date (range 1-31), Arg7=Day (range 1-7; 1=Monday, 2=Tuesday...7=Sunday)

For example, this =D command:

=D<Space><01><Space><30><Space><00><Space><04><Space><07><Space><04><Space ><07><CR>

will set the real date and time on your shaker to: 1:30:00 am, 2004, July 4, Sunday.

Biological Shakers Innova® 44/44R — Operating manual

16 Appendix B: Certifications

16.1 Certifications

The Innova 44 and 44R have been tested to ETL standards, to comply with UL and CAN/CSA electrical safety standards (see “ETL Regulatory Standards” in the specifications table).

As attested in the CE Declaration of Conformity reproduced below, the Innova 44 and 44R also conform to the appropriate CE standards.

Appendix B: Certifications

Biological Shakers Innova® 44/44R — Operating manual

Index

Accessories .....................................................................58

Alarms..............................................................................30

Appendix A ......................................................................64

Appendix B ......................................................................69

ATEX Guideline..................................................................8

Biohazard decontamination .............................................55

Calculate the offset value.................................................49

Calibrate screen...............................................................44

Caution, explanation of ......................................................7

Certifications ....................................................................69

Changing screens............................................................27

Cleaning...........................................................................55

Control Knob....................................................................25

Controls ...........................................................................25

Create a program.............................................................45

Danger, explanation of .......................................................7

Device overview

Front view...................................................................11

Internal view ...............................................................12

Rear view ...................................................................11

Display icons....................................................................29

Display screen .................................................................37

Disposal ...........................................................................63

Drain the water reservoir..................................................36

Edit a program .................................................................48

Electrical connections ......................................................34

Electrical requirements ....................................................15

Electrical Specification Plate............................................15

Environment.....................................................................15

Features...........................................................................25

Fill the water reservoir......................................................35

General overview.............................................................12

General troubleshooting ..................................................52

Getting started .................................................................32

Glide-up door ...................................................................31

Hazard symbols .................................................................7

Heater.............................................................................. 31

Inspection and unpacking of equipment.......................... 14

Inspection of boxes.......................................................... 14

Install flask clamps .......................................................... 33

Install the front grille ........................................................17

Install the stacking kit ......................................................20

Installation ....................................................................... 17

Installation of platform .....................................................32

Intended use ..................................................................... 8

Interior light(s)..................................................................31

Lamps screen.................................................................. 42

LCD display ..................................................................... 26

Level a single shaker ....................................................... 17

Level the optional base.................................................... 18

Manual conventions........................................................... 7

Mount Innova 44/44R on optional base........................... 18

Mute the audible alarm....................................................49

Name plate ...................................................................... 11

Notice, explanation of ........................................................7

Open the door.................................................................. 37

Optional gassing port kit..................................................35

Optional humidity monitor................................................35

Optional photosynthetic lamps ........................................35

Optional remote alarm..................................................... 30

Optional UV germicidal lamp ........................................... 35

Out of box concerns ........................................................14

Packing list ...................................................................... 14

Physical location.............................................................. 15

Platform assemblies ........................................................ 32

Power interruption............................................................ 50

Power Switch................................................................... 25

Preparing the location...................................................... 15

Product liability ..................................................................8

Program the shaker......................................................... 45

Programmed steps .......................................................... 45

Programs screen ............................................................. 44

Refrigeration.................................................................... 31

Remote programming ...................................................... 64

Biological Shakers Innova® 44/44R — Operating manual

Replacement parts...........................................................58

Routine maintenance.......................................................55

RS232 Screen .................................................................43

RTD .................................................................................31

Run a program.................................................................48

Safety precautions...........................................................37

Service.............................................................................31

Set the offset....................................................................49

Setup screen....................................................................40

Slide-out platform.............................................................50

Software interfaces..........................................................31

Space requirements.........................................................15

Specifications...................................................................56

Spill pan/water reservoir ..................................................31

Stack a 4400/4430 on a 44/44R ......................................24

Stack a third shaker .........................................................24

Stack two Innova 44/44R shakers ...................................22

Start the shaker ...............................................................37

Start/Stop switch..............................................................25

Summary screen..............................................................39

Symbols used ....................................................................7

Index

Temperature offset calibration .........................................49

Tools ................................................................................17

Transport and storage......................................................63

Unpacking of equipment ..................................................14

User requirement ...............................................................8

Using Calspeed ...............................................................50

Using the LCD screens....................................................37

Using this manual ..............................................................7

Warning, explanation of .....................................................7

Warranty registration........................................................14

New Brunswick Scientiﬁc • www.nbsc.com • bioinfo@nbsc.com • For your local sales ofﬁce or distributor contact:

Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 538 01-0 · Fax: +49 40 538 01-556 · E-mail: eppendorf@eppendorf.com

New Brunswick Scientiﬁc Europe B.V. · Nijmegen · The Netherlands · Tel: +31 (0) 24 3717 600 · Email: europe@nbsbv.nl

Eppendorf North America, Inc. · Hauppauge, N.Y. USA · Tel: +1 516 334 7500 · +1 800 645 3050 · E-mail: info@eppendorf.com

Application Support Europe, International: Tel: +49 1803 666 789 · E-mail: support@eppendorf.com

North America: Tel: +1 800 645 3050 menu option 2 · E-mail: techserv@eppendorf.com

Asia Paciﬁc: Tel: +603 8023 6869 · E-mail: support_asiapaciﬁc@eppendorf.com

Eppendorf 44R User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

1 User instructions

1.1 Using this manual

1.2 Danger symbols and danger levels

1.2.1 Hazard symbols

1.2.2 Degrees of danger

1.3 Symbols used

2 Safety

2.1 Intended use

2.2 User profile

2.3 Application limits

2.3.1 Description of ATEX Guideline (94/9EC)

2.4 Information on product liability

2.5 Warnings for intended use

2.5.1 Personal injury and damage to device

2.5.2 Incorrect handling of accessories

3 Product description

3.1 Main illustration

3.2 General overview

4 Inspection and unpacking of equipment

4.1 Inspection of boxes

4.2 Packing list verification

4.3 Unpacking of equipment

4.4 Out of box concerns

4.5 Warranty registration

5 Preparing the location

5.1 Physical location

5.2 Environment

5.3 Electrical requirements

5.4 Space requirements

6 Installation

6.1 Tools required for installation

6.2 Install the front grille

6.3 Level a single shaker

6.4 Level the optional base

6.5 Mount Innova 44/44R on optional base

6.6 Install the stacking kit

6.6.1 For two 44/44R shakers

6.6.2 For an Innova 4400/4430 on an Innova 44/44R

6.7 Stack two Innova 44/44R shakers

6.8 Stack a 4400/4430 on a 44/44R

6.9 Stack a third shaker

7Features

7.1 Controls

7.2 LCD display

7.3 Changing screens

7.4 Display icons

7.5 Alarms

7.5.1 Optional remote alarm

7.6 Glide-up door

7.7 Spill pan/water reservoir

7.8 Software interfaces

7.9 Interior light(s)

7.10 Heater

7.11 Refrigeration (44R only)

7.12 Service accessibility

8 Getting started

8.1 Platform assemblies

8.2 Installation of platform

8.3 Install flask clamps

8.4 Electrical connections

8.5 Optional gassing manifold kit

8.6 Optional UV germicidal lamp

8.7 Optional photosynthetic lamps

8.8 Optional humidity monitor

8.9 Fill the water reservoir

8.10 Drain the water reservoir

9 Operation

9.1 Safety precautions

9.2 Open the door

9.3 Start the shaker

9.4 Using the LCD screens

9.4.1 Display screen

9.4.2 Summary screen

9.4.3 Setup screen

9.4.4 Lamps screen

9.4.5 RS232 Screen