Carefully read this operating manual before using the device for the first time.
Also observe the operating manual enclosed with the accessories.
The operating manual should be considered as part of the product and stored in a location
that is easily accessible.
When passing the device on to third parties, be sure to include this operating manual.
If this manual is lost, please request another one. The current version can be found on our
website www.eppendorf.com.
1.2Danger symbols and danger levels
1.2.1Hazard symbols
Hazard pointBurns
Electric shockMaterial damage
1
User instructions
ExplosionHeavy loads
Inhalation
1.2.2Degrees of danger
The following degree levels are used in safety messages throughout this manual. Acquaint
yourself with each item and the potential risk if you disregard the safety message.
This device is exclusively intended for indoor use and for uniform movement and temperature
control of biological solutions and cultures in reaction vessels.
Lack of safety due to incorrect accessories or spare parts
Accessories and spare parts that are not recommended by Eppendorf compromise the safety,
CAUTION!
2.2User profile
2.3Application limits
2.3.1Description of ATEX Guideline (94/9EC)
function and precision of the device. Eppendorf cannot be held liable or accept any liability for
damage resulting from the use of non-recommended accessories and spare parts.
Only use accessories and original spare parts recommended by Eppendorf.
The device may only be operated by trained lab personnel who have carefully read this operating
manual and are familiar with the device functions.
Explosion hazard
Do not operate the device in areas where work is completed with explosive substances.
DANGER!
Do not use this device to process any explosive or highly reactive substances.
Do not use this device to process any substances which could create an explosive
atmosphere.
Due to its design and the ambient conditions in its interior, the device is not suitable for use in
potentially explosive atmospheres.
The device may only be used in a safe environment, e.g., the open atmosphere of a ventilated
lab.
The use of substances which may contribute to a potentially explosive atmosphere is not
permitted.
The final decision regarding the risks associated with using these types of substances is the
user's responsibility.
2.4Information on product liability
In the following cases, the designated protection of the device may be compromised.
The liability for the function of the device passes to the operator if:
• The device is not used in accordance with this operating manual.
• The device is used outside of the range of application described in the succeding chapters.
• The device is used with accessories or consumables that were not approved by Eppendorf.
• Service or maintenance is completed on the device by people who are not authorized by
Eppendorf.
• The owner has made unauthorized modifications to the device.
2.5Warnings for intended use
Before using the device, read this operating manual and observe the following general safety
instructions.
Lack of safety due to incorrect accessories or spare parts
Accessories and spare parts that are not recommended by Eppendorf compromise the safety,
CAUTION!
NOTICE!
function and precision of the device. Eppendorf cannot be held liable or accept any liability for
damage resulting from the use of non-recommended accessories and spare parts.
Only use accessories and original spare parts recommended by Eppendorf.
Danger due to damaged tubes
Small scratches and cracks can cause severe damage to the device and accessories. Liquids
may escape.
Before use, visually check all tubes for any damage.
The Innova 44/44R stackable incubator and incubator refrigerated shakers are large-capacity
Product description
orbital shakers that utilize a triple eccentric counter-balanced drive mechanism. They provide
horizontal plane rotary motion in either a 2.54 cm (1 in) or a 5 cm (2 in) diameter circular orbit,
depending on the model. A Proportional/Integral (PI) microprocessor controller with
instantaneous digital feedback controls the speed over the entire speed range.
The Innova 44R provides temperature control from 20 °C below ambient (as low as 4 °C) to 80
°C, and the Innova 44 from 5 °C above ambient to 80 °C. Naturally, both these ranges depend
on relative humidity and other ambient factors, as well as the options installed in the unit.
Ambient temperature is measured at one meter from the exterior of the unit.
Erlenmeyer flasks (up to 5 liters in size) as well as a wide variety of tubes and plates can be
accommodated using the New Brunswick shaker accessories described in a later section
(see Accessories on p. 58). These are easily accessed on slide-out platforms.
The Innova 44/44R may be operated in the following ways:
• Continuously: at a set speed and temperature, until user intervention.
• In a timed mode: run at a set speed, time and temperature for a period of up to 99.9 hours,
after which the shaker automatically shuts off.
• Via the shaker’s programmable controller: run through multiple temperature and speed
changes for an extended period of time.
For more details on these various modes of operation, (see Operation on p. 37).
For safe operation, the Innova 44/44R shakers are designed with a safety switch that
automatically stops the shaker mechanism when the door is opened.
After you receive your order from Eppendorf, inspect the boxes carefully for any damage that may
have occurred during shipping. Report any damage immediately to the carrier and to your local
Eppendorf Customer Service Department.
4.2Packing list verification
Verify against your Eppendorf packing list that you have received all of the correct materials.
4.3Unpacking of equipment
Risk of crushing due to missing auxiliary aid
Lifting and transporting the shaker without suitable technical aids can result in crushing and
WARNING !
other injuries.
Use a hydraulic lifting platform to install and uninstall the shaker.
To unpack your Innova 44/44R, you will need the following tools:
• Claw hammer
• Forklift or other lifting equipment to lift more than 259 kg (570 lb)
• Shears to cut 19 mm (3/4 in) wide steel strapping
• Tool to remove 7.6 cm (3 in) metal staples
Save all packing materials and this operating manual.
Inspection and unpacking of equipment
4.4Out of box concerns
If any part of your order was damaged during shipping, is missing pieces, or fails to operate
properly, please contact your Eppendorf sales representative.
4.5Warranty registration
Please contact your Eppendorf Regional Sales Office.
It is essential that the instrument be situated in an area where there is sufficient space for the
shaker and platform to clear walls and potential obstructions during operation. The surface on
which the unit is placed must be smooth, level, and able to support the shaker under full load
operating conditions.
Risk of crushing due to missing auxiliary aid
Lifting and transporting the shaker without suitable technical aids can result in crushing and
WARNING !
other injuries.
Use a hydraulic lifting platform to install and uninstall the shaker.
5.2Environment
The shaker is designed to operate optimally in the following ambient conditions:
• 10 °C to 35 °C
• 20 % to 80 % Relative Humidity (non-condensing)
5.3Electrical requirements
The Innova 44/44R can be equipped to run on:
• 100 Volts, 50 Hz, 1500 VA maximum
• 100 Volts, 60 Hz, 1500 VA maximum
• 120 Volts, 60 Hz, 1500 VA maximum
• 230 Volts, 50 Hz, 1500 VA maximum
Check your shaker’s Electrical Specification Plate (located on the back of the unit) to be sure the
electrical requirements of your unit match the output of your electric supply. If they do not match,
contact your Eppendorf representative.
Preparing the location
5.4Space requirements
Allow at least 10 cm (4 in) around the shaker for proper ventilation and for access to Power
Switch and RS-232 port accessibility on the right side. Be sure to keep the power plug and power
outlet easily accessible to facilitate unplugging the unit, as needed.
Lifting and transporting the shaker without suitable technical aids can result in crushing and
WARNING !
Hint!
6.1Tools required for installation
other injuries.
Use a hydraulic lifting platform to install and uninstall the shaker.
Feet are used on the shaker ONLY when a single unit is operated without a base. At all other
times (with two or three shakers stacked, or when a base is added), all feet must be removed.
To install the Innova 44/44R on an optional base and/or to stack Innova 44/44R units, the
following tools will be needed:
• Number 2 Phillips head screwdriver
• Blade screwdriver
• 3 mm (1/8 in) Allen key
• 3/8 in Allen key (provided)
• Level, >25.4 cm (10 in)
• Two adjustable wrenches or 7/16 in socket wrench
• Forklift or other lifting equipment able to lift more than 259 kg (570 lb)
• Metal leveling shims (provided in stacking kit)
• Optional: set of screw-in lifting handles, for small lifts of short duration (adding leveling shims,
for example) or for minor location adjustments
• Optional: stacking kit(s)
6
Installation
6.2Install the front grille
1. Remove and discard the plastic retaining clip that holds the drain hose in place for shipping.
2. Temporarily remove the thumbscrews (with their washers) installed at the bottom of each side
panel, in the corner closest to the front of the shaker.
3. Hold the front grille in place (see Fig. 4 on p. 17) and reinstall the thumbscrews (and
Abb. 4:Install the Front Grille
Fig. 4:Install the Front Grille
1 Front Grille2 Thumbscrews and Washers
washers).
6.3Level a single shaker
These instructions are for a single shaker without an added base.
Make sure that the shaker is placed on a level surface and that all four feet are solidly on the
surface. If the shaker is not level, adjust the feet as needed to achieve leveling:
1. Immobilize the top locknut against the unit with one wrench whenever you adjust the foot, to
Abb. 5:Adjustable Foot (as shipped)
keep the threaded stud from falling out (see Fig. 5 on p. 18).
6
Installation
1
2
3.65 cm
3
4
Fig. 5:Adjustable Foot (as shipped)
1Bottom of Unit2Locknut
3Flats for Wrench4Foot
2. With a second wrench against the flats of the threaded stud, just above the foot, rotate
clockwise to lower the foot or counter-clockwise to raise the foot.
3. Place a level on the top of the unit. If necessary, make further adjustments by repeating all
steps until the unit is level.
4. After installing a platform (see Getting started on p. 32), fully load the shaker and do a test run
at normal speed (see Operation on p. 37). Make additional leveling adjustments if necessary.
The maximum shaker lowering adjustment is 12.7 mm (1/2 in). The shaker cannot be raised
Hint!
above the height at which it was shipped. If more adjustment is required, you will need to add
metal shims.
6.4Level the optional base
Place the base on a sturdy (capable of bearing the weight of the combined base shakers and
shaker contents), level surface, making sure that all four corners are solidly on the surface. If the
base is not level, place metal shims as needed under the base until it is level.
6.5Mount Innova 44/44R on optional base
1. Using a forklift or lifter, raise the Innova 44/44R so that its back end is tilted toward the rear of
the base.
2. Remove all four feet from the bottom of the shaker. They will not be needed for stacking, but
you may want to keep them for future use.
3. With two assistants guiding the shaker from opposite sides, lower the unit onto the base, back
end first. Slowly and gently remove the forklift or lifter, lowering the front of the unit onto the
base by hand.
4. Remove the set screws from the bottom sides of the shaker that correspond to the mounting
holes for the rear corner braces (see Fig. 6 on p. 19).
1 Innova 44/44R2 Remove 1/2 – 13 Set Screws from holes that match
corner braces on both sides of unit.
3 1/2 – 13 x 1 1/4 in screw4 Note: Feet removed
5. Using the 1/2 – 13 x 1 1/4 in screws, lock washers and washers provided, mount the rear
braces on the base. Do not fully tighten the screws yet.
6. Mount the rear of the Innova 44/44R to the corner braces on the base, using the 1/2 – 13 x 1
1/4 in screws, lock washers and washers provided. Do not fully tighten the screws yet.
7. Mount the front corner braces to the base using two sets of the 1/2 – 13 x 1 1/4 in screws,
lock washers and washers provided. Do not fully tighten the screws yet.
8. With the remaining 1/2 – 13 x 1 1/4 in screws, lockwashers and washers, attach the front
corner braces to the unit.
9. Tighten all screws equally. With the corner braces securely in place, the shaker mounted on
its base will look as shown (see Fig. 7 on p. 20):
10. Make sure the shaker is level; add metal shims under the base as needed to level the unit.
Shims are provided with the stacking kit.
11. After installing a platform (see Getting started on p. 32), fully load the shaker and do a test run
at normal speed (see Start the shaker on p. 37). Make additional leveling adjustments if
necessary.
6.6Install the stacking kit
To stack two Innova 44/44R shakers, (see For two 44/44R shakers on p. 20). To stack an Innova
4400/4300 on an Innova 44/44R, (see For an Innova 4400/4430 on an Innova 44/44R on p. 21).
6.6.1For two 44/44R shakers
1. Remove all feet from the units (put them aside for possible future use).
2. If you do not plan to use an optional base, skip to Step 3. If you are using one, level the base
(see Level the optional base on p. 18), then mount the bottom unit on the base (see Mount Innova 44/44R on optional base on p. 18). If you are stacking three units, use a 4 in base.
3. With reference to the figure below (see Fig. 8 on p. 21), remove the set screws from the top of
the bottom unit, on the two side edges, and remove the set screws (from the sides of the unit
to be stacked) that correspond to the mounting brackets.
1 Innova 44/44R2 Remove 1/4 – 20 Set Screws before installing
stacking kit.
3 Remove set screws4 Six sets of 1/4 – 20 x 3/4 in screws, lockwashers
and washers on each side.
4. Using the screws and washers provided, attach the stacking kit mounting brackets to the top
of the bottom unit. Secure the brackets in place as shown (see Fig. 8 on p. 21).
6.6.2For an Innova 4400/4430 on an Innova 44/44R
1. Remove all feet from the units (put them aside for possible future use).
2. If you do not plan to use an optional base, skip to Step 3. If you are using one, level the base
(see Level the optional base on p. 18), then mount the bottom unit on the base as explained
in Section above (see Mount Innova 44/44R on optional base on p. 18). If you are stacking
three units, use a 4 in base.
3. With reference to the figure below (see Fig. 9 on p. 22), remove the screws installed at the top
of the bottom unit, on the two side edges.
1 Innova 4400/44302 Install right bracket with three 1/4 – 20 x 3/4 in
flathead socket screws, with two drops of Loctite
on the threads.
3 Remove screws before installing mounting
bracket.
5 Install left bracket with two sets of 10 – 32 x 1/2 in
screws, lockwashers and washers and three sets
of 1/4 – 20 x 3/4 in screws, lockwashers and
washers. Allow some play until top unit is secure
against right bracket, then tighten.
4. Use the countersunk flathead screws provided, with two drops of Loctite on their threads, to
attach the righthand (facing the door) mounting bracket to the top of the unit installed on the
base. Secure the bracket in place.
5. Use the other screws and washers provided to attach the lefthand mounting bracket to the top
of the unit installed on the base. Loosely tighten these screws, to allow some play.
6.7Stack two Innova 44/44R shakers
1. Using a forklift or lifter, raise the Innova 44/44R to be stacked so that its back end is tilted
toward the rear of the mounting brackets.
2. Remove the feet from the unit. They will not be needed for stacking, but you may want to keep
them for future use.
4 Optional Base
22
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