Eppendorf 43R User Manual

The company reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement.

TRADEMARKS:

Eppendorf® is a registered trademark, and New Brunswick™ and the New Brunswick Logo™ are trademarks of Eppendorf AG, Hamburg, Germany.

Innova USA.

Trademarks are not marked in all cases with ™ or ® in this manual. Eppendorf has attempted to identify the ownership of all trademarks from public records. Any

omissions or errors are unintentional.

is a registered trademark of New Brunswick Scientific Co., Inc. of Enfield, Connecticut,

February 3, 2013 Revision F M1320-0050

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

CAUTION!

This equipment must be operated as described in this manual. If operational guidelines are not followed, equipment damage and personal injury can occur.

Please read the entire Operating manual before attempting to use

this shaker.

Do not use this equipment in a hazardous atmosphere or with hazardous materials for which the equipment was not designed.

Eppendorf is not responsible for any damage to this equipment that may result from the use of an accessory not manufactured by Eppendorf.

New Brunswick Operating manual

TABLE OF CONTENTS

1 OVERVIEW .......................................................................................................................... 7

2 INSPECTION & UNPACKING OF EQUIPMENT ......................................................... 9

2.1 INSPECTION OF BOXES ..................................................................................................... 9

2.2 PACKING LIST VERIFICATION .......................................................................................... 9

2.3 UNPACKING OF EQUIPMENT ............................................................................................. 9

2.4 OUT OF BOX CONCERNS .................................................................................................. 9

3 PREPARING THE LOCATION ....................................................................................... 10

3.1 PHYSICAL LOCATION ..................................................................................................... 10

3.2 ENVIRONMENT ............................................................................................................... 10

3.3 ELECTRICAL REQUIREMENTS ......................................................................................... 10

3.4 SPACE REQUIREMENTS .................................................................................................. 11

3.5 NARROW DOORWAYS .................................................................................................... 11

4 INSTALLATION ................................................................................................................ 13

4.1 LEVELING THE SHAKER ................................................................................................. 13

5 FEATURES ......................................................................................................................... 15

5.1 CONTROLS ..................................................................................................................... 15

5.2 LCD DISPLAY ............................................................................................................... 16

5.3 CHANGING SCREENS ...................................................................................................... 16

5.4 DISPLAY ICONS .............................................................................................................. 17

5.5 ALARMS ......................................................................................................................... 18

5.6 L

ID ................................................................................................................................. 19

5.7 SPILL PAN/WATER RESERVOIR ...................................................................................... 19

5.8 S

5.9 I

5.10 H

5.11 R

OFTWARE INTERFACES ................................................................................................. 20

NTERIOR LIGHT(S) ........................................................................................................ 20

EATER ......................................................................................................................... 20

EFRIGERATION (43R ONLY)......................................................................................... 20

5.12 SERVICE ACCESSIBILITY ................................................................................................ 21

6 GETTING STARTED ........................................................................................................ 22

6.1 PLATFORM ASSEMBLIES ................................................................................................ 22

6.2 INSTALLATION OF PLATFORM ........................................................................................ 22

6.3 I

NSTALLATION OF CLAMPS ............................................................................................ 22

6.4 ELECTRICAL CONNECTIONS ........................................................................................... 23

6.5 O

6.6 O

PTIONAL GASSING MANIFOLD KIT .............................................................................. 24

PTIONAL GERMICIDAL UV LAMP ................................................................................ 26

6.7 OPTIONAL PHOTOSYNTHETIC LAMPS ............................................................................. 26

6.8 O

PTIONAL HUMIDITY MONITOR .................................................................................... 27

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

6.9 FILLING THE WATER RESERVOIR ................................................................................... 29

6.10 DRAINING THE WATER RESERVOIR ................................................................................ 30

7 OPERATION ...................................................................................................................... 31

7.1 SAFETY PRECAUTIONS ................................................................................................... 31

7.2 OPENING THE LID .......................................................................................................... 31

7.3 STARTING THE SHAKER .................................................................................................. 32

7.4 USING THE LCD SCREENS ............................................................................................. 32

7.4.1 Display Screen ...................................................................................................... 32

7.4.2 Summary Screen.................................................................................................... 35

7.4.3 Setup Screen .......................................................................................................... 36

7.4.4 Lamps Screen ........................................................................................................ 37

7.4.5 RS232 Screen ........................................................................................................ 39

7.4.6 Calibrate Screen ................................................................................................... 40

7.4.7 Programs Screen ................................................................................................... 41

7.5 PROGRAMMING THE SHAKER ......................................................................................... 42

7.5.1 Timer Only ............................................................................................................ 42

7.5.2 Programmed Steps ................................................................................................ 42

7.5.3 Creating a Program .............................................................................................. 42

7.5.4 Editing a Program ................................................................................................ 46

7.5.5 Running a Program............................................................................................... 46

7.6 MUTING THE AUDIBLE ALARM ...................................................................................... 46

7.7 TEMPERATURE OFFSET CALIBRATION ........................................................................... 47

7.7.1 Calculating the Offset Value ................................................................................. 47

7.7.2 Setting the Offset ................................................................................................... 47

7.8 USING CALSPEED ........................................................................................................... 48

7.9 POWER INTERRUPTION ................................................................................................... 49

8 MAINTENANCE ................................................................................................................ 50

8.1 ROUTINE MAINTENANCE ............................................................................................... 50

8.2 C

8.3 B

LEANING EXTERNAL & INTERNAL SURFACES .............................................................. 50

IOHAZARD DECONTAMINATION ................................................................................... 50

9 SERVICE & ACCESSORIES ........................................................................................... 52

9.1 T

9.2 P

9.3 O

ROUBLESHOOTING ....................................................................................................... 52

RODUCT RETURNS ........................................................................................................ 54

PENING THE SERVICE COMPARTMENT ......................................................................... 55

9.4 FUSE REPLACEMENT ...................................................................................................... 55

9.5 R

EPLACING CABINET LIGHTS ......................................................................................... 56

9.6 REPLACING OPTIONAL PHOTOSYNTHETIC LAMPS .......................................................... 57

9.7 A

9.8 R

9.9 B

DJUSTING LID TENSION ............................................................................................... 58

EPLACING OPTIONAL GERMICIDAL LAMP .................................................................... 59

ELT REPLACEMENT OR ADJUSTMENT .......................................................................... 60

9.10 REPLACEMENT PARTS .................................................................................................... 63

9.11 A

CCESSORIES ................................................................................................................. 63

9.11.1 Platforms ............................................................................................................... 63

New Brunswick Operating manual

9.11.2 Flask Clamps for Platforms .................................................................................. 64

9.11.3 Replacement Clamp Hardware Kits ..................................................................... 65

9.11.4 Test Tube Racks & Other Accessories .................................................................. 65

9.11.5 Optional Gas Manifold Kit ................................................................................... 66

9.11.6 Optional Photosynthetic Lamps ............................................................................ 66

9.11.7 Optional UV Germicidal Lamp ............................................................................. 66

9.11.8 Optional Humidity Monitor .................................................................................. 66

9.11.9 Optional Utility Basket ......................................................................................... 66

9.11.10 Optional Remote Alarm .................................................................................... 67

10 DRAWINGS, GRAPHS & TABLES ............................................................................ 68

10.1 SCHEMATICS .................................................................................................................. 68

10.2 LOAD & SPEED GRAPHS................................................................................................. 70

11 SPECIFICATIONS ......................................................................................................... 72

11.1 CERTIFICATIONS ............................................................................................................ 73

11.1.1 CE Declaration of Conformity .............................................................................. 74

12 APPENDIX: REMOTE PROGRAMMING ............................................................... 75

12.1 SETTING UP WITH HYPERTERMINAL .............................................................................. 75

12.2 OVERVIEW OF COMMAND SETS ..................................................................................... 76

12.3 INDEX TO COMMAND CODES ......................................................................................... 77

12.4 SET COMMANDS ............................................................................................................ 78

12.5 PROFILE CONTROL COMMANDS ..................................................................................... 78

12.6 REPORT REQUEST COMMANDS ...................................................................................... 80

12.7 SET/GET DATE & TIME .................................................................................................. 81

13 INDEX .............................................................................................................................. 82

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

The Innova 43/43R Incubator Shakers are large-capacity orbital shakers that utilize an eccentric counterbalanced drive mechanism. They provide horizontal plane rotary motion in either a 1-inch (2.54 cm) or a 2-inch (5 cm) diameter circular orbit, depending on the model. A Proportional/Integral (PI) microprocessor controller with instantaneous digital feedback controls the speed over the entire speed range.

The Innova 43R provides temperature control from 20º below ambient (as low as 4ºC) to 80C, and the Innova 43 from 5º above ambient to 80ºC. Naturally, both these ranges depend on relative humidity and other ambient factors, as well as the options installed in the unit. Ambient temperature is measured at one meter from the exterior of the unit.

Erlenmeyer flasks (up to 6 liters in size) as well as a wide variety of tubes and plates can be accommodated using the New Brunswick shaker accessories described in Section 9.11. These are easily accessed on the platform.

To facilitate moving the unit, each shaker is equipped with casters that roll freely when the feet are raised.

The Innova 43/43R may be operated in the following ways:

 Continuously: at a set speed and temperature, until user intervention.  In a timed mode: run at a set speed, time and temperature for a period of up to 99.9

hours, after which the shaker automatically shuts off.

 Via the shaker’s programmable controller: run through multiple temperature and

speed changes for an extended period of time.

 Via computer through an RS-232 interface.

See Section 7 for more details on these various modes of operation.

For safe operation, the Innova 43/43R shakers are designed with a safety switch that automatically stops the shaker mechanism when the lid is opened.

The Innova 43/43R is equipped with visual and/or audible alarms that alert the user to the following conditions:

 The end of a timed run  Deviations from speed setpoint  Deviations from temperature setpoint  Power failure  Lid open

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New Brunswick Operating manual

To accommodate customer needs, a wide variety of platforms can be used with the Innova 43/43R:

 Universal platforms are the most flexible, providing hole patterns for flask clamps,

test tube racks and other accessories.

 Dedicated platforms are supplied with flask clamps attached; they are designed solely

and expressly for this purpose.

 Test tube racks, microplate holders, and test tube rack holders are also available (a

universal platform is needed for all test tube racks and holders).

For further information on these accessories, see Section 9.11.

We also recommend that you consult the load and speed graphs provided in Section 10.2.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

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2.1 Inspection of Boxes

After you receive your order from Eppendorf, inspect the boxes carefully for any damage that may have occurred during shipping. Report any damage immediately to the carrier and to your local Eppendorf Customer Service Department.

2.2 Packing List Verification

Verify against your packing list that you have received all of the correct materials.

2.3 Unpacking of Equipment

CRUSH WARNING!

Do not attempt to lift the Innova 43/43R by hand. Always use a lifter or other suitable equipment when raising or handling the unit.

To unpack your Innova 43/43R, you will need the following tools:

 Claw hammer  Scissors to cut nylon strapping  Tool to remove 3-inch (7.6 cm) metal staples

Save all packing materials and this user’s guide/operating manual.

2.4 Out of Box Concerns

If any part of your order was damaged during shipping, is missing pieces, or fails to

operate properly, please fill out the Customer Satisfaction Form 6300, included with your

equipment, and return it by fax.

New Brunswick Operating manual

3.1 Physical Location

It is essential that the instrument be situated in an area where there is sufficient space for the shaker to clear walls and potential obstructions during operation. The surface on which the unit is placed must be smooth, level, and able to support the shaker under full load operating conditions.

CRUSH WARNING!

Do not attempt to lift the Innova 43/43R by hand. Always use a lifter or other suitable equipment when raising or handling the shaker.

NOTE:

The Innova 43/43R has casters and leveling feet. When you need to move the shaker across the floor, make sure the feet are raised enough so that the casters can roll freely without scraping the floor with the feet. At all other times, make sure the feet are down, to avoid any unintended rolling.

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3.2 Environment

The shaker is designed to operate optimally in the following ambient conditions:

 10º to 35°C  20 to 80% Relative Humidity (non-condensing)

3.3 Electrical Requirements

The Innova 43/43R can be equipped to run on:

 100 Volts, 50 or 60 Hz, 1500 VA maximum  120 Volts, 60 Hz, 1500 VA maximum  230 Volts, 50 Hz, 1500 VA maximum

Check your shaker’s Electrical Specification Plate (located on the back of the shaker) to be sure the electrical requirements of your shaker match the output of your electric supply. If they do not match, contact your Eppendorf representative.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

3.4 Space Requirements

Allow at least four inches (10 cm) around the shaker for proper ventilation and for access to Power Switch and RS-232 port accessibility on the right side. Also allow enough

room above the shaker for the lid to be fully open (see Figure 1).

Be sure to keep the power plug and power outlet easily accessible to facilitate unplugging the shaker, as needed.

Figure 1: Innova 43/43R Front View

1 Depth: 32 inches (81 cm) including front panel protrusions 4 Pedal to open lid 2 Width: 45.5 inches (115.6 cm) 5 Handle to open lid 3 Height: 40.6 inches (103 cm) with feet

NOTE: Open lid adds 23.5 inches (59.7 cm)

NOTE:

Be sure to allow at least four inches (10.5 cm) around shaker for ventilation, access to power cord (rear panel), and access to power switch and RS-232 port (right side).

3.5 Narrow Doorways

If you need to move the Innova 43/43R through a doorway that is less than 32.5 inches (82.5 cm) wide, remove the front window cover to reduce the depth of the shaker to 30.3 inches (77 cm):

New Brunswick Operating manual

1. Carefully remove the five screws on the underside of the plastic window cover,

retaining them for reuse.

2. Being very careful not to hit the control knob or button, slide the window cover

upward to remove it from the spring clips on the front of the shaker.

When the shaker is in the desired location, reinstall the window cover by reversing the procedure.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

CRUSH WARNING!

Do not attempt to lift the Innova 43/43R by hand. Always use a lifter or other suitable equilment when raising or handling the shaker.

4.1 Leveling the Shaker

NOTE:

Make sure that the shaker is placed on a level surface, in its intended location. Lower all four adjustable feet as described below until they are solidly on the surface. If the shaker is not level, adjust the feet as needed to achieve leveling:

1. Immobilize the top lock nut against the shaker with one wrench whenever you

adjust the foot, to keep the threaded stud from falling out (see Figure 2).

Figure 2: Adjustable Foot (as shipped)

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1 Bottom of shaker 3 1.75 inch (4.45 cm) 5 Foot 2 Lock nut 4 Flats for wrench

2. With a second wrench against the flats of the threaded stud, just above the foot.

Rotate clockwise to lower the foot or counter-clockwise to raise the foot.

New Brunswick Operating manual

3. Place a level on the top of the shaker. If necessary, make further adjustments by

repeating all steps until the shaker is level.

4. After installing a platform (see Section 6), fully load the shaker and do a test run at

normal speed (see Section 7). Make additional leveling adjustments if necessary.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

5.1 Controls

1 Shaking Start/Stop switch 3 RS-232 port 5 Control knob 2 Display 4 Power switch

 START/STOP SWITCH

 CONTROL KNOB

 RS-232 PORT



POWER SWITCH

Figure 3: Front Panel (detail)

This switch is used to start or stop the shaker. It will also activate the timer when a timed run is desired. If the shaker is stopped and restarted, the timer automatically returns to the beginning of a run.

This knob is multifunctional. It is used to change screens, and to select and change operating conditions.

See Section 5.8 for details.

This rocker switch is a circuit breaker that turns power on and off to the entire Innova 43/43R.

EEAATTUURREES

NOTE:

In addition to the power switch, the power cord is also used to conduct power or to break the power circuit to the shaker. Whenever power to the shaker may be a hazard (during cleaning, maintenance or service work), be sure to disconnect the power cord from the electrical outlet.

New Brunswick Operating manual

5.2 LCD Display

When the shaker is powered up, using the Power Switch located on the right side of the

machine (see Figure 3), the initial display screen will remain for a short time while the

system boots up. Then the main screen, called This screen will indicate the same parameters that were in effect when the power was turned off.

DISP for Display, appears (see Figure 4).

Figure 4: Display Screen

ºC 23

RPM 120

 For an explanation of the icons on the display screen, see Section 5.4.  Turning the Control Knob will highlight functions and/or values that can be changed.  Whenever you turn the Control Knob, the chamber light will go on (for details, see

For more information on working in the Display Screen, see Section 7.4.1.

DISP We 13 : 44

1 Parameters 5 Program running 2 Temperature offset 6 Heater on 3 Actual parameter values 7 Lid open 4 Day & 24-hour time 8 Parameters locked 9 Audible alarm muted 10 Screen name

Section 5.9).

5.3 Changing Screens

You can change screens displayed by highlighting the screen name field in the lower left corner, pressing the Control Knob in until it clicks, rotating the Knob left or right (which also makes clicking sounds) to the desired screen and clicking the Control Knob in again. Table 1 describes the various screens:

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Table 1: Screens

Screen

Meaning Features/Modes

Name

DISP Display Shows two user-selectable parameters* and actual values.

SUMM Summary Shows all parameters*, setpoints and actual values.

SET Set-Up Set day of week, set time, enable or mute alarm, lock or

unlock operating parameters

LAMP Lamps

COMM Communication

(RS-232)

CAL Calibration Allows user to enter a temperature offset.

PROG Program Allows user to set up 1- 4 programs, each with 1-15 steps.

* see Table 2, Display Screen Parameters  Optional Not installed

Internal chamber light: ON (always on); OFF (always off); AUTO (default mode), light goes on and stays on when lid is open, shuts off 15 seconds after lid is closed, and goes on for 15 seconds when Control Knob is moved.

Photosynthetic lights (GRO): ON, OFF, UV Light (UV): ON, OFF,

SET: set baud rate OFF disables RS-232 MONITOR: PC commands shaker to read setpoints and actual values on a schedule determined by PC software. Parameters are unlocked and can be changed by program or manually. SLAVE: PC controls shaker and logs data. TALK: Shaker sends setpoint and actual data to PC at one-minute intervals.

Self-calibrates the speed sensor.



NONE



NONE

Table 2: Display Screen Parameters

Parameter Name Meaning

RPM Shaking speed, in revolutions/minute

ºC Chamber temperature, in degrees Celsius

HRS Programmed time remaining, in hours

 %RH Relative Humidity, in percent.  UV Status of Ultraviolet germicidal lamp  GRO Status of Photosynthetic growth lamps

 Optional

5.4 Display Icons

There are six icons that help identify operating conditions. Five of these icons are located at the bottom of the display, and they are visible, when applicable, in any screen you are viewing, except the Program subscreens. The sixth appears, when applicable, next to ºC whenever the temperature is onscreen.

New Brunswick Operating manual

Table 3: Display Icons

Icon Explanation

This icon appears when audible alarms are muted.

This icon appears when the possibility to make manual or programmed parameter changes is disabled (locked). This is controlled by settings on the SET screen. See Section 7.4.3

This icon appears when the shaker lid is open.

This icon appears when the Heater is on. See Section 5.10.

This icon appears when a user-defined Program is running. See Section 7.5.5.

This icon appears to the right of ºC if the Temperature Offset feature is being used. See Section 7.7.

5.5 Alarms

If an alarm condition exists, the field in the lower right corner will alternate the Day and Time with characters indicating the nature of the alarm condition, accompanied by an audible alarm (unless muted):

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Table 4: Alarms

Indication Description

TEMP

SPEED

POWER

HRS Indicates when timed run is completed.

The temperature deviates more than 1ºC from setpoint after achieving control temperature range. After lid is opened, alarm will be disabled for 5 minutes while chamber recovers to setpoint. The speed deviates more than 5 RPM from setpoint after achieving operating speed setpoint. After lid is opened, alarm will be disabled for 5 minutes while chamber recovers to setpoint. Indicates shaker is powering up (both at normal power-up and after power interruption); will flash until the Control Knob is moved.

5.6 Lid

Access to the chamber of the Innova 43/43R is through the top-opening hinged lid. To

open the lid, firmly press the pedal or lift up on the front handle (see Figure 1). The lid

stands on its hinges until you choose to close it.

When the lid opens, the following will happen:

 Heater turns off  Shaker stops  Interior light goes on and, when it is in AUTO mode, will remain on for 15

seconds after the lid is closed

 UV germicidal lamp (if so equipped) turns off

5.7 Spill Pan/Water Reservoir

The Innova 43/43R is equipped with a spill pan and spill cover to protect the drive mechanism in the case of accidental spills and/or broken glassware. This pan can also be used as a water reservoir to humidify the chamber and to reduce evaporation. An optional factory-installed humidity monitor is also available.

The reservoir can be drained through the quick-connect valve on the front of the shaker.

New Brunswick Operating manual

5.8 Software Interfaces

The RS-232 port is next to the Power Switch on the right side of the control panel (see Figure 3). It can be used to interface a computer to the shaker for control of operating

conditions or data logging applications (see Section 12).

The customer is responsible for securing the proper driver to interface with the RS-232.

5.9 Interior Light(s)

When the

LAMP screen is in its default AUTO mode, the interior (“chamber”) light is

activated for 15 seconds whenever you turn the Control Knob. It will automatically shut off after 15 seconds of Control Knob inactivity.

The chamber light will also go on when the lid is open.

In addition, you can set the chamber light to be continuously either mode in the LAMP screen.

There are two additional light options for refrigerated shakers only: interior

photosynthetic growth lamps (see Section 6.7) and a germicidal UV lamp located outside the chamber but in the airflow path (see Section 6.6).

ON or OFF by selecting

5.10 Heater

The chamber temperature is sensed by a 1000 ohm platinum RTD. A 650W heater is controlled using pulse width modulation on a 2.5-second duty cycle. This cycle time is fast enough to prevent noticeable changes in air temperature due to the cycling.

Whenever the heater is on, the Heater On icon

will appear in the display. The

heater automatically stops running when the lid is opened.

NOTE:

Operating the shaker for sustained periods at temperatures above 60°C may result in a shorter fan lifespan.

5.11 Refrigeration (43R Only)

The refrigeration system in the Innova 43R is a variable-capacity system carefully designed with self-checks to maintain the setpoint, to balance pressure within the system, and to prevent freezing on the evaporator surface.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

When the shaker is powered up, there is a four-minute time delay prior to compressor start-up.

5.12 Service Accessibility

In the unlikely event that your Innova 43/43R should need service, all electronic boards,

refrigeration, heating components are easily accessed, by an authorized service technician, behind the right side panel of the shaker.

New Brunswick Operating manual

6.1 Platform Assemblies

The Innova 43/43R can be used with a variety of New Brunswick platforms that will

accept a wide range of clamps for flasks, test tubes, etc. A platform, which is required for operation, is a separate item, not included with the shaker assembly. Refer to

Section 9.11 for details on available platforms and platform accessories.

6.2 Installation of Platform

Prior to use, a spill cover and platform must be installed on the shaker. The shaker is shipped with four Allen head platform screws installed in the bearing housing (see Figure 5 below, which also shows the spill cover that you must install).

Figure 5: Installing Platform & Spill Cover

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1 Platform screws 3 Spill cover 2 Platform 4 Bearing housing

6.3 Installation of Clamps

Flask clamps purchased for use with universal platforms (see Section 9.11.2) require

installation. Clamps are installed by securing the base of the clamp to the platform with the correct type and number of screws. All clamps are shipped complete with hardware.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Clamps for 2-, 2.8-, 4- and 6-liter flasks are shipped with an additional girdle to keep the

flasks in place (see Figure 6). To install these double girdle clamps:

1. Place clamp on platform, secure in place with correct type of screws: #S2116-3051,

10-24 x 5/16-inch flat Phillips head, provided with the clamps (see Figure 6a).

2. Place the loose girdle around the upper portion of clamp body so that it is held in

place by the legs of the clamp.

3. Insert the flask into the clamp, and push the girdle down so the rubber tubes are in

contact with the platform and the flask.

Figure 6: Double Girdle Clamp Installation

1 Platform 4 Clamp body (legs & base) 2 Clamp mounting holes (5) 5 Lower girdle with girdle tubes 3 Upper girdle with girdle tubes

Figure 6a: Clamp Fastener

6.4 Electrical Connections

Before making electrical connections, verify that the power source voltage matches the voltage on the electrical specification plate and that the position. The electrical specification plate is located on the rear panel of the shaker near the power connector.



ON/OFF switch is in the OFF

New Brunswick Operating manual

Connect the power cord to the power connector, then connect the other end to a suitable, grounded receptacle. Make sure there is enough clearance to disconnect the plug whenever necessary.

Figure 7: Rear Panel

1 Power connector 2 Exhaust vents: do not block!! 3 Adjustable foot

6.5 Optional Gassing Manifold Kit

This option is factory-installed. The manifold delivers gas into the chamber via as many as 12 ports (see Figures 8 & 9 on the following page). The manifold can be

adapted to the desired tubing configuration by adding or subtracting ports or by temporarily clamping off unused tubes. You may elect to use splitters (barbed Yconnectors) after the manifold to increase the number of flasks you can serve.

You determine the appropriate gas flow rate using a pressure regulator (which you supply) on the gas supply.

NOTE:

Gas supply should be regulated and it must never exceed 15 PSIG.

50 feet of 1/16-inch (ID) sterilizable silicone tubing is supplied with the kit. Filters may be needed: 0.22μ syringe filters (which you supply) can be fitted to the individual manifold ports to maintain a sterile barrier.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Figure 8: Gas Monitor (optional)

Figure 9: Gas Fitting (with optional Gas Manifold)

1 Gas manifold connection (1/4 FNPT)

SAFETY PRECAUTIONS!

 Never use the gas manifold with flammable gases.  Never exceed 15 PSIG inlet pressure to the manifold.  Make sure free lengths of tubing are secured by the hook and loop straps to keep them from contact with flasks or other moving parts.

New Brunswick Operating manual

6.6 Optional Germicidal UV Lamp

WARNING!

Never try to operate the UV Germicidal Lamp while the shaker lid is open.

This option, a germicidal ultraviolet lamp, is placed inside the Service Compartment (see Figure 10), outside the chamber to help reduce the risk of contamination. The lamp is

identified on the display screen as UV.

Figure 10: Germicidal UV Lamps (optional)

1 Front of shaker

The UV germicidal lamp is factory-installed and available on refrigerated shakers only. Operation instructions are provided with this option (see Section 9.11.7).

6.7 Optional Photosynthetic Lamps

This factory-installed option, available on refrigerated shakers only, provides nine

photosynthetic growth lamps inside the chamber (see Figure 11 on the following page).

They can be turned on and off manually, by the easily set programmable timer, or by computer. These lamps are identified on the display screen as GRO. The replacement bulb part number is P0300-0221.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

The recommended operating temperature when using this option is 15 - 37ºC; the maximum operating temperature is 70ºC.

Figure 11: Photosynthetic Lamps (optional)

1 Photosynthetic lamp assembly

6.8 Optional Humidity Monitor

This optional factory-installed accessory allows you to monitor relative humidity levels in the chamber throughout your run.

New Brunswick Operating manual

Figure 12a: Humidity Monitor (optional)

1 Humidity monitor

When the humidity sensor is present in the chamber, maximum shaker temperature is automatically limited to 60°C.

Specifications:

Humidity operating range: 0 to 100% RH Accuracy: See Figure 12b Temperature operating range: 4 to 60ºC Hysteresis: ± 1.5% RH Recovery time after saturation: 10 seconds Long term stability: 0.5% RH per year

Figure 12b: RH Sensor Operating Range & Humidity Error Graph

100

+/- 5 % E rro r

INTERMITTENT

OPERATION

+/- 3 % E rro r

+/- 5 % E rro r

0 102030405060

Temperature (C)

% RH

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Figure 12c: RH Sensor Connection

M1282-7002

M1282-7012

1 Board inside the service compartment

Figure 12d: Humidity Sensor Mounting

1 Inside wall of cabinet

CAUTION!

Operating the Innova 43/43R at temperatures above 60ºC can result in permanent damage to the sensor.

Water can be added to the spill pan in order to elevate the humidity level in the chamber.

See Section 6.9 for instructions.

6.9 Filling the Water Reservoir

If you choose to use the spill pan as a water reservoir to reduce evaporation and to raise the humidity level in the chamber:

New Brunswick Operating manual

1. Open the lid and temporarily remove the platform.

2. Make sure that the drain check valve is closed.

NOTE:

As you add water, do not allow the water to splash or flow into the recessed middle of the pan, where the bearing housing assembly is mounted. Pour water very slowly into the shallow area beyond the edge of the spill cover to protect the bearing housing.

3. Accessing the pan/reservoir from the left, the right or in front of the spill cover,

slowly fill the reservoir with no more than 3 liters of distilled water. A long, narrow

watering can or a flexible hose will make it easier to access the pan while protecting the bearing housing from accidental overflow.

At a 37°C setpoint, the chamber loses approximately 500 mL/hr from the pan. At a 25°C setpoint, and placed in a 25°C room, the chamber achieves a relative humidity equilibrium that is approximately 15% above the ambient humidity.

6.10 Draining the Water Reservoir

To drain water from the water reservoir/spill pan:

1. Attach the quick-connect drain fitting, direct it to a container or drain, and allow the

water to gravity drain.

2. When the reservoir is empty, detach the fitting.

The spill pan/water reservoir drain is located in the front, on the left, under the humidity tray.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

7.1 Safety Precautions

Before operating the shaker, verify that anyone involved with its operation has been instructed in both general safety practices for laboratories and specific safety practices for this apparatus.

The user is also responsible for following local guidelines for handling hazardous waste and biohazardous materials that may be generated from the use of this equipment.

If service should be required on a shaker that is going to be returned to an Eppendorf facility, it must be completely decontaminated and cleaned prior to its return.

It is the responsibility of the user to carry out appropriate decontamination procedures if hazardous material is spilled on or inside the equipment. Before using any cleaning or decontamination method other than those suggested by the manufacturer, users should check with Eppendorf that the proposed method would not damage the equipment.

This equipment is not “explosion-proof” and should never be used with flammable

substances or used to grow organisms that produce flammable by-products.

PPEERRAATTIIOON

CAUTION!

To prevent damage to the shaker and its contents, never run the shaker without a platform.

7.2 Opening the Lid

Open the lid by firmly pressing the foot pedal or lifting up on the front handle. The lid will stand open on its hinges until you swing it down to the closed position.

New Brunswick Operating manual

7.3 Starting the Shaker

To initially start the shaker, close the lid and turn the power switch (located on the righthand side of the control panel) to the ON position. The display will come on (first showing only or

43R, and the stroke, 1 inch or 2 inch, and then quickly moving into the Display

New Brunswick Scientific, then briefly displaying the model number, 43

screen), and the audible alarm will sound. To mute it, see Section 7.6.

When the shaker begins to operate, the LCD display will track the speed as it accelerates to the last entered setpoint. The shaking action may be started or stopped by pressing the Start/Stop button on the front panel.

NOTE:

The shaker will not operate if the lid is open. This is indicated by the “lid open” symbol appearing in the bottom line of the display (see Figure 4 or Table 3).

7.4 Using the LCD Screens

7.4.1 Display Screen

When you turn the power on, this is the first screen to appear after the company title screen. The default display parameters are temperature (ºC) and shaking speed (RPM).

You can change the displayed parameters. To replace a parameter:

1. Using the Control Knob, highlight the parameter that you wish to replace. For

this example, we will replace RPM (see Figure 13).

NOTE:

If you highlight an item, change it, but do not save your selection, after a few seconds the screen will revert to its previous setting.

NOTE:

“UV” (UV Germicidal lamp) and “GRO” (photosynthetic growth lamps) will appear in this screen with the word “NONE” if your shaker is not equipped with these optional features.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Figure 13: Changing Display Parameter

ºC 23

RPM 120

DISP We 13:44

2. Click the Control Knob in.

3. Turn the Knob until the desired parameter appears in the highlighted field.

For this example, we will select

4. Click the Knob in, to set and save the parameter (see Figure 14).

Figure 14: Changed Display Parameter

RPM will flash.

HRS.

ºC 23

HRS 0.0

DISP We 13:44

You can also use this screen to verify a setpoint, even though the values displayed

here are actual (current) values. To view a setpoint:

1. Use the Control Knob to highlight the value (in this example, we will view the

temperature setpoint, so we will highlight the current

2. Click the Knob in to display the current setpoint, which will flash.

At this point you can modify the setpoint or click the Knob in again to return to the normal display, which will be the actual temperature.

ºC, which is 23).

New Brunswick Operating manual

To modify a setpoint in this screen:

1. Use the Control Knob to highlight the current value (we will continue to use

the temperature as our example, so we will select 23).

2. Click the Knob in to display the current setpoint (in this example, 38.5—see

Figure 15), which will flash.

Figure 15: Changing Setpoint

ºC 38.5

RPM 120

DISP We 13:44

3. Turn or spin the Knob to reset the setpoint (in this example, turn the Knob left

to decrease the setpoint to 37.0).

NOTE:

If you turn the Control Knob slowly, one click left or right will change the setpoint by an increment of one tenth of a degree Celsius (0.1ºC). If you spin the Knob, the value will change by larger increments.

4. Click the Knob in to set and save this new setpoint.

NOTE:

If you highlight an item, change it, but do not save your selection, after a few seconds the screen will revert to its previous setting.

5. The display will automatically return to the actual value.

To move out of this screen and into the next:

1. Use the Control Knob to highlight DISP, then click the Knob in. DISP begins

to flash.

2. Turn the Knob to the right until the next screen, Summary (

If you turn too far and enter another screen, just turn the Knob back to the left to recapture the

3. Click the Knob in to select the screen and to work in it.

SUMM screen.

SUMM), appears.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

7.4.2 Summary Screen

In this screen (see Figure 16), you can see both the current ACTUAL readings and

the SETpoints for shaking speed (RPM), chamber temperature (ºC), elapsed time in a programmed run (

(see Section 9.11.8), the percentage of relative humidity (

PARAM ACTUAL SET

HRS) and, if you are using the optional Humidity Monitor

%RH).

Figure 16: Summary Screen

RPM 100 100 ºC 45.1 45.0 HRS 0.0 0.0 %RH 50.0 N/A

SUMM Th 16:18

NOTE:

The current day (Su, Mo, Tu, We, Th, Fr or Sa) and time always remains visible in the lower righthand corner.

The only elements you can modify in this screen are setpoints. To change setpoints in this screen:

1. Turn the Knob until the desired setpoint is highlighted, then click the Knob in.

The setpoint will begin to flash.

2. Turn the Knob to the right to increase the number, or to the left to decrease it.

One click left or right will increase the setpoint by an increment of one (one whole unit or one tenth unit, depending on the parameter). Move the Knob more rapidly (you can spin it) to change the value by larger increments.

3. Click the Knob in to set and save the new value.

NOTE:

If you highlight an item, change it, but do not save your selection, after a few seconds the screen will revert to its previous setting.

4. Repeat the above steps to change any or all of the other setpoints.

New Brunswick Operating manual

To move out of this screen and into the next:

1. Use the Control Knob to highlight SUMM, then click the Knob in. SUMM

begins to flash.

2. Turn the Knob to the right until the next screen, Setup (SET), appears. If you

turn too far and enter another screen, just turn the Knob back to the left to recapture the SET screen.

3. Click the Knob in to select the screen and to work in it.

7.4.3 Setup Screen

Here you can set the day of the week and the time (on a 24-hour clock). This screen also allows you to lock all of your settings from further changes, and to mute or enable the audible alarm.

Figure 17: Setup Screen

SETUP

Day : Thu Hour/Min : 16:19 Lock : Off Mute : Off

SET Th 16:19

To change the day:

1. Turn the knob to highlight the day (Thu in the sample screen above), then

click inward once. The day will flash.

2. Turn the knob left or right to select the day of choice: Sun, Mon, Tue, Wed,

Thu, Fri or Sat.

3. Click the knob in to set and save your choice.

NOTE:

If you highlight an item, change it, but do not save your selection, after a few seconds the screen will revert to its previous setting.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

To change the time (Hour/Min):

1. Turn the knob to highlight the time (16:19 in the sample screen above), then

click inward once. The time will flash.

2. Turn the knob left or right to change the time. Left moves backward, right

moves forward in time. One click right or left changes by one minute; spin the knob to move more rapidly.

3. Click the knob once inward to set and save your choice.

To lock the settings:

1. Turn the knob to highlight Lock, then click inward once. The current status

(Off in the sample screen above) will flash.

2. Turn the knob in either direction; the only other choice is

inward to select and save

On, or continue turning to return to Off.

On. Click once

3. When you set Lock to On, a padlock icon (see Section 5.4) will appear at the

bottom of the screen. This icon will remain on display through all main display screens until you turn the locking function off.

To mute the audible alarm:

1. Turn the knob to highlight Mute, then click inward once. The current status

(Off in the sample screen above) will flash.

2. Turn the knob in either direction; the only other choice is On. Click once

inward to select and save On, or continue turning to return to Off.

3. When you set Mute to On, the icon will appear at the bottom of the

screen. This icon will remain on display through all screens until you turn the muting function off.

To move out of this screen and into the next:

1. Use the Control Knob to highlight

SET, then click the Knob in. SET begins to

flash.

2. Turn the Knob to the right until the next screen, Lamps (

you turn too far and enter another screen, just turn the Knob back to the left to recapture the

LAMP screen.

3. Click the Knob in to select the screen and to work in it.

7.4.4 Lamps Screen

In this screen (see Figure 18), you can turn the chamber light (Chamber), the

optional UV germicidal (“decontamination”) lamp ( photosynthetic growth lamps (

Growth) on and off.

LAMP), appears. If

UV Decont) and the optional

New Brunswick Operating manual

On means the light is always on, and Off means the light is always off, unless you

add additional programming.

There is an additional mode for the chamber light: Auto. In Auto mode, the light will go on every time you activate the control knob or open the lid. This is the default mode.

NOTE:

The Lamps screen, shown below, will always indicate Chamber light mode. If the shaker is not equipped with the optional UV germicidal lamp and/or photosynthetic growth lamps, UV Decont and/or Growth will say None.

Figure 18: Lamps Screen

LAMPS

Chamber : Auto UV Decont : Off Growth : Off

LAMP Th 16:20

To change the mode setting for any of the lamps:

1. Turn the Control Knob to highlight the setting for the lamp of choice, then

click the knob in. The current setting will flash (in the sample screen, we will use the

2. Turn the Knob left or right until the desired mode setting appears (Auto in this

example).

3. Click the Knob in to save the new setting.

NOTE:

If you highlight an item, change it, but do not save your selection, after a few seconds the screen will revert to its previous setting.

To move out of this screen and into the next:

1. Use the Control Knob to highlight

begins to flash.

Chamber light as an example.

LAMP, then click the Knob in. LAMP

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

2. Turn the Knob to the right until the next screen, RS232 (COMM), appears. If

you turn too far and enter another screen, just turn the Knob back to the left to recapture the COMM screen.

3. Click the Knob in to select the screen and to work in it.

7.4.5 RS232 Screen

This screen (see Figure 19) is used only if you have connected a personal computer to the RS-232 port (see Section 5.8). Here you can select the RS-232

port’s Mode and the Baud Rate appropriate to your PC.

Figure 19: RS232 Screen

RS232

Mode : Off Baud Rate : 19200

F/W Rev : D/H

COMM Th 16:21

1 FirmWare Revision level (this line is for information only) 2 In this sample screen, the Display is at Revision D and the FirmWare Control Board is at

Revision H.

To change the Communication Mode:

1. Turn the Control Knob to highlight the current setting (

screen above), then click the Knob in. The current setting will flash.

2. Turn the Control Knob left or right until the desired mode setting appears (see

Table 5: Communication Mode, on the following page).

Off in the sample

New Brunswick Operating manual

Table 5: Communication Mode

Mode Application

Off The RS-232 port is not open to communication in

either direction.

Slave The shaker can be fully controlled from the

computer.

Talk The shaker sends current value reports to the

computer once per minute.

Monit [Monitor] The shaker responds only to “Report Requests”.

3. Click the control knob inward once to save the new setting.

NOTE:

If you highlight an item, change it, but do not save your selection, after a few seconds the screen will revert to its previous setting.

To change the Baud Rate:

1. Turn the control knob to highlight the current setting (19200 in the sample

screen above), then click the knob inward once. The current setting will flash.

2. Turn the control knob left or right until the desired setting appears: 9600,

19200 or 38400. The setting you choose should match the baud rate of your computer.

3. Click the control knob inward once to save the new setting.

To move out of this screen and into the next:

1. Use the Control Knob to highlight COMM, then click the Knob in. COMM

begins to flash.

2. Turn the Knob to the right until the next screen, Calibrate (

CAL), appears. If

you turn too far and enter another screen, just turn the Knob back to the left to recapture the CAL screen.

3. Click the Knob in to select the screen and to work in it.

7.4.6 Calibrate Screen

Use this screen (see Figure 20 on the following page) to create a temperature offset and to calibrate the shaking speed (for details, see Sections 7.7, Temperature Offset Calibration, and 0, Using Calspeed).

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Figure 20: Calibrate Screen

CALIBRATE

°C Offset : 0.0 Calspeed : 250

CAL Th 16:22

1 This is a sample of the Indicated RPM Value.

7.4.7 Programs Screen

Use this screen (see Figure 21) to set as many as four operating programs for the

shaker. Each program can have as many as 15 steps. See Section 7.5 below for complete details.

Figure 21: Programs Screen

PROGRAMS

Program 1 : Off Program 2 : Off Program 3 : Off Program 4 : Off

PROG Th 16:23

New Brunswick Operating manual

7.5 Programming the Shaker

7.5.1 Timer Only

By setting an HRS setpoint in the DISP or SUMM screen, the shaker may be set to automatically stop after a preset time period of 0.1 to 99.9 hours. If the time is set to 0.0, the shaker will operate continuously until either the lid is opened or the Start/Stop button is pushed.

7.5.2 Programmed Steps

The resident software for the Innova 43/43R can store up to four programs, each

having as many as 15 steps. Each step can be programmed in one-minute

increments, for total periods of one minute up to 99 hours 59 minutes each.

To enter the programming mode, use the Control Knob to select the PROG screen

(see Figure 21, repeated below). At this point, you can Run a program, Edit a

program, generate a New program or turn a program Off. Off is the default mode.

Figure 21: Programs Screen

PROGRAMS

Program 1 : Off Program 2 : Off Program 3 : Off Program 4 : Off

PROG Th 16:23

7.5.3 Creating a Program

To write a New program:

1. Use the Control Knob to highlight the mode of Program 1 (in our sample

screen, Figure 21, it is to flash.

Off), then click the Knob in. The selected field begins

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

2. Turn the Knob until the field says New. Click the Knob in to select this

mode. The screen for Program 1 - Step 1 will open (see Figure 22):

Figure 22: Program 1 – Step 1

PRG1 - STEP -- 01

Time 00:00 ºC 20.0 RPM Off UV Off GRO Off

 Save Cancel

1 Step number 2 Use “Cancel” to leave the programming mode without saving any new settings. 3 Use “Save” to leave the programming mode and save all new settings. Do NOT use

“Save” until the programming is complete.

4 Use these arrows to scroll through the programming steps.

NOTE:

If your shaker is not equipped with these optional features, “UV” and “GRO” will appear with the word “Off” in this screen but will not be programmable.

3. Turn the Knob to highlight the Time setting (00:00 in the sample screen

above), then click the Knob in. The field will flash.

4. Turn the Knob until the desired running duration for this step (from 00:01,

which means one minute, to 99:59) appears, then click the Knob in to save the

setting. We will set Step 1 time to eight hours for this example (see Figure 23).

5. Turn the Knob to highlight the

screen above), then click the Knob in. The field will flash.

6. To set the temperature desired (ºC from 4.0 to 80.0) for the time period you

have set, turn the Knob (left to decrease, right to increase). When the desired value appears, click the Knob in to save the setting. We will set Step 1

temperature to 37.0ºC (see Figure 23).

ºC temperature setting (20.0 in the sample

New Brunswick Operating manual

7. Turn the Knob to highlight the RPM setting (Off in the sample screen above),

then click the Knob in. The field will flash.

8. Turn the Knob to select the desired shaking speed (25 to 400 RPM) for this

time period, then click the Knob in to save the setting. We will set Step 1

speed to 150 RPM (see Figure 23).

9. If you do not have the optional UV lamp, skip this step. If you have the UV lamp

(default setting is Off) and you wish to have it on during the time period you have set, use the Control Knob to select the field, click the Knob to select the field (it will flash), turn the Knob to select On, then click the Knob in to save

the setting. In our sample Step 1 (see Figure 23), however, UV remains Off.

10. If you do not have the optional GRO lamps, skip this step. If you have the

photosynthetic lamps (default setting is Off) and you wish to have them on during the time period you have set, use the Control Knob to select the field, click the Knob to select the field (it will flash), turn the Knob to select On,

then click the Knob in to save the setting. In our sample Step 1 (see Figure

18), however, GRO remains Off.

NOTE:

DO NOT select “Save” yet!

11. To program Step 2 (see sample screen in Figure 23): use the Control Knob to

highlight the arrows at the bottom left of the screen. Click the Knob to make the arrows flash, then turn the Knob to the right until Step 2 appears. Click the Knob in to work in this screen, and repeat Steps 3-10.

Figure 23: Program 1 – Step 2

PRG1 - STEP 01 02

Time 08:00 03:00 ºC 37.0 40.0 RPM 150 225 UV Off Off GRO Off Off

 Save Cancel

1 Current step number

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Whatever step is shown on the right-hand side of the display is settable (in Figure 23, it is Step 2). To scroll between steps, select the arrows (bottom left), then turn the Control Knob clockwise or counterclockwise, then select the desired step.

NOTE:

The time entered for each step is for that step only; it is not cumulative (i.e., the elapsed time from the start of the program).

12. Continue to program as many as 15 steps in the same manner. For our sample

program, we have only three steps (see Figure 24).

Figure 24: Program 1 – Step 3

PRG1 - STEP 02 03

Time 03:00 12:00 ºC 40.0 4.0 RPM 225 0 UV Off Off GRO Off Off

 Save Cancel

The three-step program we have illustrated is designed to start incubating cultures at 37ºC and hold that temperature for eight hours, shaking at 150 RPM. After eight hours, Step 2 engages, increasing the temperature setpoint to 40ºC for a temperature induction, holding that temperature for three hours and increasing the shaking speed to 225 RPM. After this interval, the temperature will be cooled to 4ºC and held at that temperature for twelve hours; no shaking will occur during this period, as the speed is set to 0 RPM.

Please note that temperature reductions, even at temperatures above ambient (going from 40º to 30ºC, for example), require refrigeration to be effective.

To save the entire program:

1. Once all your steps are set, use the Control Knob to highlight Save at the

bottom of the screen, then click the Knob in. The field will flash.

2. Click the Knob in again to save the program. The display will read

Running – Saving Profile

PROG) screen.

(

If you wish, you can set Programs 2, 3 and 4 and save them in the same manner.

for a few seconds, then return to the main Programs

Process

New Brunswick Operating manual

7.5.4 Editing a Program

Use the Edit function to open a program you have already created and saved, in

order to modify your settings following the same procedures.

7.5.5 Running a Program

Use the Run fuction to turn a specific program on. Naturally, only one program

can run at a time. When you change the mode to Run, the screen will show the

Run icon ( ):

Figure 25: Programs Screen – Running

PROGRAMS

Program 1 : Off Program 2 : Run Program 3 : Off Program 4 : Off

PROG Fr 08:31

To stop a program: You can abort a run any time by changing the program’s

function to Off.

7.6 Muting the Audible Alarm

The Innova 43/43R shakers have an audible alarm that is activated under predetermined

conditions (see Table 4). It may be muted in the following way:

1. Turn the Control Knob until the

Knob in to work in this screen.

2. Turn the Knob to highlight the

the alarm is audible). To mute the alarm, click the Knob in. The field will flash.

3. Turn the Knob to change the setting to

4. To reactivate the audible alarm at any time, repeat steps 1-3, reversing

SET screen is highlighted on the display. Click the

Mute mode (the default setting is Off, which means

On, then click the Knob to save this selection.

Off and On.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

7.7 Temperature Offset Calibration

The temperature probe and the temperature controller are calibrated together at the factory. The temperature probe measures the temperature of the air at the probe’s location, near the return vent. The controller uses the probe input to adjust air temperature, up or down, to match the temperature setpoint. Depending on various conditions within the chamber, such as flask placement and size, the heat produced by growing organisms, heat losses due to liquid evaporation from flasks, etc., the display temperature may differ from temperatures within the flasks themselves. You can calculate the correction value for this offset and program the shaker to display a corrected temperature.

7.7.1 Calculating the Offset Value

If you wish to have the temperature display (“Indicated Temperature”) match the temperature at a given point, or match the average of a series of points within the chamber (“Actual Temperature”), proceed as follows:

1. Let the shaker equilibrate at or near the desired temperature, then record the

Indicated Temperature.

2. Now record the Actual Temperature.

3. Calculate the temperature correction value using this formula:

Actual Temperature – Indicated Temperature = Temperature Offset Value.

4. To set the Temperature Calibration Offset, follow the procedure outlined in

Section 7.7.2 below.

7.7.2 Setting the Offset

To set the temperature calibration

Offset:

1. Use the Control Knob to enter the CAL screen (see Figure 20, repeated

below).

2. Turn the Control Knob to highlight the current setting (

0.0 in the sample

screen above), then click the Knob in. The current setting will flash.

3. Turn the Knob (left for negative settings or right for positive settings) to

display the desired setting. As you turn the Knob, each click represents one tenth of a degree Celsius (0.1ºC).

4. When you reach the desired setting, click the Control Knob in to save the new

value.

New Brunswick Operating manual

5. When the Temperature Offset is set to any value other than 0, the icon

will appear next to ºC in the DISP and SUMM screens.

NOTE:

If you highlight an item, change it, but do not save your selection, after a few seconds the screen will revert to its previous setting.

Figure 20: Calibrate Screen

CALIBRATE

°C Offset : 0.0 Calspeed : 250

CAL Th 16:22

7.8 Using Calspeed

The Calspeed function, which is set in the CAL screen (see Figure 20 above), is used to

calibrate the speed of the shaking mechanism. Calibrated at the factory, speed does not need to be recalibrated until a major operating component (e.g., drive belt) is changed.

NOTE:

Prior to calibrating the speed, make sure that the platform is properly secured to the subplatform, and any flasks present are secured.

When the shaker is running, the to calibrate the speed, set the speed to a value that can be measured— a setpoint of 250 RPM works well. The use of a strobe is recommended for accuracy. If, after measuring the actual speed, you wish to adjust the indicated value:

1. Click the Control Knob in.

2. Set the new value.

3. Click the Knob in again to save the setting.

4. Turn the shaker

OFF, wait a few seconds, then turn the shaker back ON.

CAL screen shows the indicated RPM value. If you wish

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

7.9 Power Interruption

In the event of a power failure, the Innova 43/43R Shakers are equipped with an

automatic restart function. The shaker’s non-volatile memory retains all stored

information.

If the shaker was in operation prior to the power interruption, the shaker will begin to operate at its last entered setpoints. The alarm/POWER display will flash, indicating that a power interruption has occurred. Turn the Control Knob in any direction to acknowledge the visual alarm. The flashing will stop.

New Brunswick Operating manual

WARNING!

When cleaning the shaker, always turn off the shaker and disconnect the power cord from the power supply.

8.1 Routine Maintenance

No routine maintenance schedule is required for the Innova 43 and 43R.

To ensure that your shaker retains its attractive appearance, an occasional cleaning, using

a cloth with conventional household (non-abrasive) cleaner is recommended (see Section

8.2 below for more details).

We also suggest that the area around the shaker be vacuumed or swept to remove dust and other debris, ensuring proper air flow in and around the shaker.

AAIINNTTEENNAANNCCE

8.2 Cleaning External & Internal Surfaces

The shaker may be cleaned using a damp cloth or any standard household or laboratory cleaner to wipe down its outer surfaces. Do not use abrasive or corrosive compounds to clean this instrument, as they may damage the shaker and void the warranty.

If Biohazard decontamination is required, see Section 8.3 below.

8.3 Biohazard Decontamination

not damage the equipment.

Commercially available household bleach solutions, when diluted at a 1:10 ratio, are effective in routine decontamination of the instrument. The method for decontaminating a spill depends upon the nature of the spill.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Spills involving fresh cultures or samples known to have low concentrations of biomass should be flooded with decontamination solution and soaked for 5 min before cleanup. Spills involving samples with high concentrations of biomass, or involving organic matter, or occurring in areas warmer than room ambient temperature, should be exposed

to decontamination solution for at least one hour before cleanup.

WARNING!

Personnel involved in the cleanup of any spill should wear gloves, safety glasses, and a laboratory coat or gown during the cleanup process. Respiratory protection should be considered for spills where aerosolization is suspected.

New Brunswick Operating manual

9.1 Troubleshooting

If any problems occur with your shaker, do not attempt to perform any service on the shaker other than specified in this manual. Unauthorized servicing may void the warranty. Please contact your local Eppendorf Customer Service Department

In any correspondence with Eppendorf, please refer to the model number and serial number of your shaker. This information is on the electrical specification plate, located on the rear panel of the shaker, above the power connector.

There are some problems, however, that you can investigate and correct yourself. Refer to the following Troubleshooting Guide:

Symptom(s) Probable Cause(s) & Solution(s)

Shaker does not run.

Shaker runs slowly and/or no speed indication.

No power; display is not on; power cord is not plugged in and/or power switch is off: plug in power cord (to working electric outlet), and turn on power switch. Lid is open—look for Open Lid icon on display: close the lid firmly. Lid is closed but Open Lid icon is on display: lid switch is broken; call for service. On/Off switch is not working: call for service. Fuse(s) burned out: check and replace as needed. Shaking speed has been set to Zero by program running (look for Run icon in display) or by computer interface: reset shaking speed. Defective main board: call for service Defective display controller board: call for service. Jammed shaking mechanism: call for service Defective motor: call for service Drive belt out of alignment or worn: call for service. Fuse(s) burned out: check and replace as needed. Incorrect speed calibration: recalibrate shaking speed. Defective main board: call for service. Defective motor: call for service. Drive belt out of alignment or worn: call for service.

EERRVVIICCEE

...continued...

CCCCEESSSSOORRIIEES

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Symptom(s) Probable Cause(s) & Solution(s)

Shaker does not run at set speed.

Operating noise

Incubator does not reach set temperature.

Incorrect temperature indication.

Incorrect relative humidity %

Shaker is running in Program mode (look for Run icon in display). Shaker speed has been changed by RS-232 command/ computer interface. Shaker is overloaded: remove some contents & balance load. Defective motor: call for service. Drive belt out of alignment or worn: call for service. Load out of balance: unload all contents from platform, then reload. Loose component(s) in Platform, subplatform, and/or drive assembly: call for service. Shaker running in Program mode: Run icon in display. Temperature setpoint changed by RS-232/computer interface command. Heater fuse blown: replace. Compressor fuse blown: replace. Compressor over-pressure switch activated: call for service. Chamber fans not working: call for service Ambient temperature too high or too low: cool or heat the room as needed. Lid is not completely closed (even though Open Lid icon may not be on display): open it and reclose it firmly. Line voltage is too low. Frequency on line voltage if set incorrectly: reset. Defective heater: call for service. Defective refrigeration system: call for service. Incorrect temperature indication (see below).

Temperature Offset has been programmed (look for  adjacent to C on display). Defective RTD assembly: call for service. Defective main board: call for service. Defective humidity probe or broken contact: call for service.

...continued...

New Brunswick Operating manual

Symptom(s) Probable Cause(s) & Solution(s)

Photosynthetic GRO lamp does not work.

UV germicidal lamp does not work.

Shaker is running in Program mode (look for Run icon in display), and program calls for GRO lamp to be off. GRO lamp mode has been changed by RS-232 command/ computer interface. Bulb is burned out; replace (see Section 9.6). Fuse is burned out: replace (see Section 9.4) Check ballast voltage. Check cable connections. Check switch settings. GRO lamp mode has been changed by RS-232 command/computer interface. Shaker is running in Program mode (look for Run icon in display), and program calls for UV lamp to be off. UV lamp is burned out; replace (see Section 0) Fuse is burned out: replace (see Section 9.4) Check ballast voltage. Check cable connections. Check switch settings. UV lamp mode has been changed by RS-232 command/ computer interface.

9.2 Product Returns

Should you need to return your Innova 43/43R to Eppendorf for any reason. Contact Eppendorf Customer for return instructions.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

9.3 Opening the Service Compartment

WARNING!

Before opening the Service Compartment, always turn off the shaker and disconnect the power cord from the power supply.

The Service Compartment (see Figure 26) contains the shaker’s electronic and

temperature control components. The user should access the Service Compartment only

to replace a fuse—see Section 9.4 for Fuse Replacement instructions). All other service intervention are to be performed by authorized service technicians only.

9.4 Fuse Replacement

WARNING!

When replacing fuses, always turn off the shaker and disconnect the power cord from the power supply.

There are four fuses that can be replaced by the user. These are located inside the

Service Compartment. To access the fuses (see Figure 26):

1. Turn the power off and unplug the shaker.

2. Remove the four screws and washers that hold the right side service panel grille in

place. Set them aside for reuse.

3. Fuses are numbered 1 through 4 (see Table 6); access the fuse you wish to replace by

using a coin or a blade screwdriver to turn and release the spring-loaded cap.

4. Replace the fuse with a new one of the same type and rating (see Table 6 below):

Table 6: Fuses

Fuse No. Function Type Rating

F1 Heater F2 GRO Lamps (photosynthetic) 3.15 A F3 UV Lamp (germicidal) 3.15 A F4 Compressor 10 A

 optional equipment

Slo Blo

8 A

New Brunswick Operating manual

Figure 26: Fuse Replacement

1 Fuses (F1-F4) 2 Service Compartment

9.5 Replacing Cabinet Lights

WARNING!

Before installing or removing the quartz bulbs: (1) Make sure power is disconnected from the shaker, (2) Wear plastic gloves to protect the bulb from skin oils and to protect your hands just in case the bulb should break, (3) Before removal, make sure the bulb has fully cooled.

NOTE:

Cabinet lights are connected in series. When one burns out, both lights will be off. It is therefore prudent to replace both bulbs at the same time.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Be sure to follow all the WARNINGS and the NOTE above as well as the procedure

outlined below when you replace the cabinet bulbs with reference to Figure 27 below:

Figure 27: Replacing Cabinet Lights

1. Make sure power is disconnected from the shaker.

2. Open the lid.

3. Wearing plastic gloves, remove the first used bulb, then carefully insert the new

bulb’s prongs fully into the connector base.

4. Repeat step 3 for the second bulb.

9.6 Replacing Optional Photosynthetic Lamps

WARNING!

Before removing or installing the photosynthetic “GRO” lamp, make sure power is disconnected from the shaker.

To replace the photosynthetic GRO lamp bulbs, with reference to Figure 28:

1. Make sure power is disconnected from the shaker.

2. Open the lid.

New Brunswick Operating manual

3. Remove the two thumbscrews from the diffuser holder.

Figure 28: Replacing Photosynthetic Lamps

4. Remove the diffuser by placing a finger underneath and rotating the diffuser in the

slot at the back of the photosynthetic light assembly. All 9 lamps should now be accessible to you.

5. To remove the lamp for replacement, rotate the lamp 90º, taking care to support the

lamp before it drops down.

6. To replace the lamp, orient the pins of the new lamp vertically. Simultaneously,

gently press both sides of the lamp into the slots.

7. Rotate the new lamp 90º. The lamp should catch in the holders on both ends. Make

sure both ends are secure.

8. Put the diffuser back in place, reinstalling the diffuser holder with its two

thumbscrews.

9.7 Adjusting Lid Tension

WARNING!

The following procedures are provided for your information only. Do not attempt to perform these service interventions yourself unless you are an authorized service technician.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

In the event the lid does not stay in the fully open position, you may want to adjust the lid tension: tighten the 5/16-inch nuts on the hinges to increase spring tension.

9.8 Replacing Optional Germicidal Lamp

WARNING!

Before installing or removing the germicidal “UV” lamp, the authorized technician will: (1) Make sure power is completely disconnected from the shaker, (2) Wear plastic gloves to protect the lamp from skin oils and to protect your hands just in case the lamp should break, (3) Before removal, make sure the lamp has fully cooled.

To replace the UV lamp, your service technician will follow all the WARNINGS above and the procedure and WARNINGS outlined below, with reference to Figure 29:

1. Make sure power is disconnected from the shaker.

2. Open the Service Compartment by removing the side panel.

3. Free the heater cable harness and the temperature probe from their tie wraps and

Permagum®.

Figure 29: Germicidal (UV) Lamp Replacement

NOTE that wires, tie wraps and Permagum® are not shown above.

4. Remove the evaporator cover from the Service Compartment, paying careful attention

to the drain tubes.

New Brunswick Operating manual

5. Wearing plastic gloves, remove the germicidal lamp by pressing it toward the spring-

loaded tombstone. The lamp has a single pin at each end.

6. Still wearing plastic gloves, install a new lamp.

7. Pull the heater cable harness and temperature probe back through the holes in the

Service Compartment.

8. Replace the fan cover, making sure the heater cable harness wires and temperature

probe have been pulled through. Adjust the temperature probe position to ensure that the probe tip can protrude 3 – 3.25 inches (7.6 – 8.2 cm) from the fan cover face.

9. Secure the heater cable harness and temperature probe with tie wraps, and add

Permagum® to fill the hole.

10. Reinstall the side panel that closes off the Service Compartment.

11. Use the plastic fitting to hold the temperature probe, ensuring that its tip is 3 – 3.25

inches (7.6 – 8.2 cm) from the fan cover face.

9.9 Belt Replacement or Adjustment

To gain access to the drive belt, your service technician will follow these steps with

reference to Figures 30 & 31 below:

1. Make sure that the power is switched off and the shaker is unplugged.

2. Open the lid and remove the platform.

3. Using a 7/32-inch Allen key (hex wrench), remove the four bolts that hold the

subplatform in place, and remove the subplatform.

4. Disconnect the motor wire by unplugging its connector.

5. With a wrench, remove the nuts that hold the bearing housing to the bottom

weldment.

6. Position the counterweight so it is closest to you, and tie it in place with tie wraps or

strong cord, in order to prevent it from dropping against your fingers or causing you to lose your grip.

7. Remove the bearing housing with care: it weighs 47 lbs. / 21.3 kg.

8. Turn the bearing housing over to access the belt, motor and pulley.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Figure 30: Drive Assembly

1 Spill deflector 2 Drive assembly 3 Bearing housing

To replace the drive belt, follow these steps with reference to Figures 30 & 31:

1. Loosen the four hex nuts that hold the motor assembly in place and remove the old belt.

2. Guide the belt onto the two pulley grooves.

3. Tighten the motor nuts.

4. Check the belt adjustment by applying finger pressure to the belt midway between the

two pulleys. The belt should deflect approximately 3/8-inch (9-10 mm).

5. If the deflection is not right, loosen the nuts, readjust the belt, then retighten the

motor nuts.

New Brunswick Operating manual

Figure 31: Belt Replacement & Adjustment

1 Drive belt 4 Four motor fixing bolts (loosen for belt tensioning) 2 Pulley 5 Motor 3 Bearing housing

If an adjustment is required:

1. Loosen the four hex nuts on the motor assembly.

2. Move the motor assembly until the belt is tight.

3. Tighten the hex nuts and recheck the belt tension by exerting pressure on the belt.

The belt should deflect approximately 3/8-inch (9.5 mm).

Reassembly:

1. In reverse order, reinstall the bearing housing. Once it is securely in place, remove

the tie wraps/cord.

2. Secure the wiring to ensure no interference during rotation.

3. Reinstall the subplatform and platform.

4. Recalibrate speed (see Section 0).

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

9.10 Replacement Parts

In Table 7 below you will find commonly needed replacement parts for your Innova 43/43R shaker:

Table 7: Replacement Parts

Part Description Quantity Part No.

Spare Parts Kit (includes 1 motor drive poly-V belt, two each hinge assemblies, halogen bulbs, and 10A, 8A, & 3.15A fuses) Spare Parts Kit (includes 1 motor drive poly-V belt, and two each 10A, 8A, & 3.15A fuses) Flask Clamp screws, 10-24 x 5/16-inch flat Phillips head (+) Test Tube Rack screws, 10-24 x ½-inch pan head, and washers (#10 plain and #10 lock washers) Motor Drive Poly-V Belt, 28 inches long 1 P0700-7070 Fuse, 10A 1 P0380-3162 Fuse, 8A 1 P0380-3630 Fuse, 3.15A 1 P0380-3460 Chamber Light Bulbs 2 P0300-0470 UV Germicidal Bulb (if so equipped) 1 P0300-0351 Photosynthetic Bulb (if so equipped) 1 P0300-0221 UV Germicidal Light Ballast (if so equipped) 1 P0420-5113 Photosynthetic Light Ballast (if so equipped) 1 P0420-5192

1 M1320-6000

1 M1282-6000

25 S2116-3051

10 each M1289-0800

9.11 Accessories

When ordering accessories, you may be asked to provide the model number and serial number of your shaker. This information is on the electrical specification plate, located on the rear panel of the shaker. The serial number is also labeled in the lower right corner of the front panel, below the chamber lid seal.

9.11.1 Platforms

Table 8 lists the various solid 18- x 30-inch (45.7 x 76 cm) platforms available for your Innova 43/43R. Consult Section 10.2 for load and speed graphs.

Table 8: Available Platforms

Description Part No.

Universal Platform M1250-9920 50 mL Erlenmeyer flask Dedicated Platform M1191-9908 125 mL Erlenmeyer flask Dedicated Platform M1191-9909 250 mL Erlenmeyer flask Dedicated Platform M1191-9910

...continued...

New Brunswick Operating manual

Description Part No.

500 mL Erlenmeyer flask Dedicated Platform M1191-9911 1L Erlenmeyer flask Dedicated Platform AG-1 2L Erlenmeyer flask Dedicated Platform AG-2

2.8L Fernbach flask Dedicated Platform AG-28 4L Erlenmeyer flask Dedicated Platform AG-4 6L Erlenmeyer flask Dedicated Platform AG-6

To add your choice, following is a list of flask capacities for the Universal Platform and for the dedicated platforms designed solely for one flask size:

Table 9: Platform Flask Capacities

Flask Type Universal Platform

Dedicated Platform

Capacity*

10 ml Erlenmeyer Flasks 183 N/A 25 ml Erlenmeyer Flasks 92 N/A

50 ml Erlenmeyer Flasks 92 108 125 ml Erlenmeyer Flasks 39 60 250 ml Erlenmeyer Flasks 30 40 500 ml Erlenmeyer Flasks 18 24

1 L Erlenmeyer Flasks 12 15 2 L Erlenmeyer Flasks 8 12

2.8 L Erlenmeyer Flasks 6 6 4 L Erlenmeyer Flasks 6 6 5 L Erlenmeyer Flasks 6 6 6 L Erlenmeyer Flasks 4 4

*Clamps are sold separately.

Capacity*

9.11.2 Flask Clamps for Platforms

The following clamps are available for the matching Dedicated Platform; all may be used on the Universal Platform:

Table 10: Flask Clamps

Clamp Size Part Number

10 ml Erlenmeyer Flask ACE-10S 25 ml Erlenmeyer Flask M1190-9004 50 ml Erlenmeyer Flask M1190-9000 125 ml Erlenmeyer Flask M1190-9001 250 ml Erlenmeyer Flask M1190-9002 500 ml Erlenmeyer Flask M1190-9003 1 L Erlenmeyer Flask ACE-1000S 2 L Erlenmeyer Flask ACE-2000S

...continued...

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Clamp Size Part Number

2.8 L Fernbach Flask ACFE-2800S 4 L Erlenmeyer Flask ACE-4000S 5 L Erlenmeyer Flask ACE-5000S 6 L Erlenmeyer Flask ACE-6000S

9.11.3 Replacement Clamp Hardware Kits

New Brunswick flask clamps come complete with mounting screws. Additional

screws are available separately in packs of 25 (see Section 9.10).

9.11.4 Test Tube Racks & Other Accessories

Table 11: Racks & Trays

Accessory Description Part

Adjustable angle Test Tube Rack for tubes 8 – 11 mm diameter

Adjustable angle Test Tube Rack for tubes 12 - 15 mm diameter

Adjustable angle Test Tube Rack for tubes 15 –18 mm diameter

Adjustable angle Test Tube Rack for tubes 18 – 21 mm diameter

Adjustable angle Test Tube Rack for tubes 22 – 26 mm diameter

Adjustable angle Test Tube Rack for tubes 26 - 30 mm diameter

Microplate holder rack (stacked) Microplate holder rack (single layer) Angled Test Tube Rack Holder* for user-supplied test tube racks that are 4-5 in. (10-13 mm) wide and up to 15 in. (38 mm) long. Angled Test Tube Rack Spacer Bar* for use with TTR-210 to accommodate test tubes racks that are less than 5 in. (13 mm) wide.

* Universal Platform Required

80 tube capacity M1289-0100 7 60 tube capacity M1289-0010 9 48 tube capacity M1289-0001 9 60 tube capacity M1289-0200 7 44 tube capacity M1289-0020 9 34 tube capacity M1289-0002 9 42 tube capacity M1289-0300 7 31 tube capacity M1289-0030 9 24 tube capacity M1289-0003 9 30 tube capacity M1289-0400 7 23 tube capacity M1289-0040 9 18 tube capacity M1289-0004 9 22 tube capacity M1289-0500 7 16 tube capacity M1289-0050 9 13 tube capacity M1289-0005 9 20 tube capacity M1289-0600 7 16 tube capacity M1289-0060 9 12 tube capacity M1289-0006 9 3 deep well or 9 standard M1289-0700 16

5 deep well or standard TTR-221 4

Number

TTR-210

TTR-215

Platform Capacity

New Brunswick Operating manual

9.11.5 Optional Gas Manifold Kit

Table 12: Gas Manifold Kit (Factory-Installed)

Description Part Number

Gas Manifold Kit M1320-0500

9.11.6 Optional Photosynthetic Lamps Table 13: Photosynthetic Growth Lamps (Factory-Installed)

Description Part Number

Photosynthetic Growth Lamp Assembly (9 lamps) M1320-0300

9.11.7 Optional UV Germicidal Lamp

Table 14: Germicidal Lamp (Factory-Installed)

Description Part Number

UV Germicidal Lamp Assembly M1320-0400

9.11.8 Optional Humidity Monitor

Table 15: Humidity Monitor (Factory-Installed)

Description Part Number

Humidity Monitor (for use with spill pan as water reservoir)

9.11.9 Optional Utility Basket

Table 16: Utility Basket

Description Part Number

Utility Basket M1320-0700

M1320-0600

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Figure 32: Utility Basket (optional)

9.11.10 Optional Remote Alarm

The Innova 43/43R can be equipped with a factory-installed remote alarm component (part number M1320-8029). When it is hooked up to your relay and receiving requipment, this device will send notification of an alarm condition to the remote location you choose.

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110

10.1 Schematics

Figure 33a: Control Schematics (left side)

load

Filter

AC POWER

ENTRY

100-230 VAC

FROM REFRIG

GND STUD

HEATER 230Vac OPERATION

HEATER 120Vac OPERATION

LF1 line

MAIN GND STUD

M1320-8013

M1320-8016

RED

BLACK

WHITE

YELLOW

RED

BLACK

WHITE

YELLOW

WIINNGGSS

RRAAW

CB001 15 AMP

11 12

M1335-8000

POWER

TRANSFORMER

ONLY FOR

230V & 100V

INPUT LINE

I43R REFRIGERATION UNIT

(OPTIONAL)

TB001

M1320-8013

RED

WHITE

LINE INPUT

JUMPER

230V

120V

100V

RED

M1320-8004

WHITE



INSTALL

P0380-5980

5 3 1

M1335-8002

RRAAPPHHSS

LINE INPUT

VOLTAGE SELECT

230V

J25

J22

J20

POWER DISTRIBUTION

J17

120V 2

J21

BOARD

M1282-7011

50/60 SELECT

60HZ

(100V)

(120V)

100V

J13

50HZ

(100V)

(230V)

J12

J11

J18

J14

J1-12

AABBLLEES

2 3

HEATER 100Vac OPERATION

I43R REFRIGERATION UNIT

(OPTIONAL)

COND. FAN

OVER PRESSURE SWITCH

Comp.Unit

REFRIGERATIO N GND STUD

RED

BLACK

WHITE

YELLOW

TO MAIN GND STUD

BLU BLK ORG BLK

TB001

RED

WHITE

J7-2 (+30V)

J21

LOW PWR.

M1335-8007

FAN

EXT.

A C

SSR

+5V

M1320-8021

GND

TB002

BLU

ORG

BLK

VIO

BLK

VIO

3 4

1 3

J19

M1320-8027

Alarm Option



Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Figure 33b: Control Schematics (right side)



POWER DISTRIBUTION

BOARD

M1282-7011

J12

J11

J14

J7-2 (+30V)

J1-12

M1320-8000

BRN

PNK

2 3

M1320-8022

120V

GND

M1320-8033

5 ohm, 25W

BLK

WHT

5 3

CN1

POWER SUPPLY

P0460-3330

Temp.

Sensor,RTD

50 Cond. Ribbon Cable

M1320-8020

Door

Switch

TB001

M1320-8011

M1320-8010

BALLAST

(OPTIONAL)

CN2

LAMP

M1320-8024

LAMP

BLUE

RED

5 ohm, 25W

1 2

3 4

LAMP

J5J18

CONTROL BOARD

BRN

J6A J6B

M1282-7012

7.5 ohm, 25W

(ONLY NON-REFIG.)

(M1320-8032)

PNK

M1282-8019

PNK

BRN

TB001

M1320-8031

3 4 5 6 7

8 9

M1320-8006

10 Cond.

Ribbon Cable

M1320-8007

14 Cond.

Ribbon Cable

P1-1 P1-2 P1-3 P1-4 P1-5 P1-6 P1-7 P1-8

RS232

Port

PHASE C PHASE B PHASE A

GND VREF

SA SB SC

M1282-5010

PINK

FAN 1

BRN

PINK

FAN 2

BRN

PINK

FAN 3

BRN

MTR

M001

ENCODER

P0400-1911

SWITCH ROTARY

DISPLAY BOARD

M1282-7010

M1320-8017

SWITCH ASSY

PUSH BUTTON

START

STOP

+5V

GND

Humidity

Sensor

(OPTIONAL)

M1320-8006

CN1

Display

LCD

M1320-8025

M1282-8016

1 2

J19

M1320-8027

GND

M1282-5046

Alarm Option

PHOTOSYNTHETIC

LIGHT BANK M1282-5046

(OPTIONAL)



New Brunswick Operating manual

Figure 34: Refrigeration Schematic

10.2 Load & Speed Graphs

The following graphs explain the maximum recommended shaking speed according to the load you put on your shaker’s platform and the diameter of the shaker’s orbit (1-inch or 2-inch).

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Figure 35: Load & Speed for 1-Inch Orbit Shakers

(20% Full)

500 450 400 350 300 250 200 150

Maximum Recommended RPM

100

300

250

200

50, 125, 250, 50

1L,

2.8L

2L, 4L, 6L

<20 20 to 30 30 to 36 36 to 40 40 to 50 50 to 60 >60

Platform and Load (lb)

Figure 36: Load & Speed for 2-Inch Orbit Shakers

(20% Full)

150

50, 125, 250,

500 mL

1L,

2.8L

2L, 4L, 6L

100

Maximum Recommended RPM

<20 20 to 30 30 to 36 36 to 40 40 to 50 50 to 60 >60

Platform and Load (lb)

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111

PPEECCIIFFIICCAATTIIOONNS

These specifications assume a maximum load of 34 pounds (15.5 kg), including platforms,

clamps, glassware and contents.

Innova 43 & 43R Incubator Shaker

SHAKING Speed

Control Accuracy Indication Stroke/Orbit TEMPERATURE Range (43) Range (43R) Control Accuracy Uniformity Indication Heater

ALARMS

LCD DISPLAY RS-232 SETPOINT RETENTION AUTOMATIC RESTART DRIVE

DRIVE MOTOR SAFETY

ELECTRICAL REQUIREMENTS

ETL REGULATORY STANDARDS

CE REGULATORY STANDARDS

DIMENSIONS3 Width Depth (Front to Back) Height

see first NOTE on following page

depending on ambient factors, such as relative humidity, and options installed

see second NOTE on following page

20-500 rpm with 1-inch (2.5 cm) stroke or 20-300 rpm with 2-inch (5.1 cm) stroke  1 rpm Displayed in 1 rpm increments 1-inch (2.5 cm) or 2-inch (5.1 cm)

5ºC above ambient temperature to 80°C 20ºC below ambient temperature (minimum 4ºC) to 80°C

 0.1°C from 30-40 °C,  0.5°C over the remaining range.  0.25°C in flask across platform at 37ºC, and  0. 5°C (or less) at 60ºC

Displayed in 0.1°C increments Long-life, low-watt density resistance-type heater with high temperature thermostat Visible and audible warning indication when speed deviates more than 5 rpm or temperature more than 1°C from setpoints, and when timer has expired. Audible alarm can be muted. 240 x 128 backlit Remote control, remote monitoring, remote data logging All setpoints and operating status are retained in non-volatile memory Automatic restart after power is restored, indicated by flashing display Triple-eccentric counterbalanced drive with nine permanently lubricated ball bearings and poly-V belt. Self-calibrating. Solid-state brushless DC motor. Drive Interrupt shuts off power to Shaker when lid opens. Acceleration/deceleration circuit prevents sudden starts and stops, minimizing both splashing and mechanical damage.

 100V, 50Hz  100V, 60 Hz  120 Volts, 60 Hz  230 Volts, 50 Hz

UL 61010A-1 CAN/CSA-C22.2 No. 1010.1 UL 61010A-2-010 CAN/CSA-C22.2 No. 1010.2.010

IEC 61010-1: 2001 2nd ed. EN 61010-2-081 (2002) EN 61000-3-2 : 1995 EN 61010-1: 2001 2nd ed. EN 61326 : 1997 + A1 : 1998 EN 61000-3-3 : 1995 EN 61010-2-010 (1992) + A1: 1996

53 inches (135 cm)

32 inches (81 cm)

40.6 inches (103 cm)

43: 800 VA per shaker

43R: 1500 VA per shaker

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Innova 43 & 43R Incubator Shaker

CHAMBER DIMENSIONS CHAMBER LID PLATFORM WEIGHT 43 43R

24 inches deep X 34.5 inches wide X 19 inches high from top of platform (61 cm deep X 87.6 cm wide X 48 cm high from top of platform)

Foot-activated lid opening. Aluminum, 18" X 30" (46 X 76 cm). Select universal or dedicated styles

460 lbs (209 kg) 510 lbs (231 kg)

NOTE:

Use of baffled flasks will significantly reduce maximum speed for any shaker.

11.1 Certifications

The Innova 43 and 43R have been tested to ETL standards, to comply with UL and CAN/CSA electrical safety standards (see “ETL Regulatory Standards” in the specifications table).

As attested in the CE Declaration of Conformity reproduced on the following page, the

Innova 43 and 43R also conform to the appropriate CE standards (see also “CE Regulatory Standards” in the specifications table).

New Brunswick Operating manual

11.1.1 CE Declaration of Conformity

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

112

12.1 Setting Up with HyperTerminal

You can use the Microsoft® HyperTerminal program on your personal computer to communicate with your shaker:

1. Before you set up HyperTerminal, be sure to check the Mode and Baud Rate

parameter settings (see Section 7.4.5).

2. While you are in the RS232 screen (see Figure 15), set the

and select the appropriate is 19200; your other choices are 9600 or 38400.

3. Connect the RS-232 cable between your computer and the shaker’s RS-232 port (see

Figure 4).

4. Start HyperTerminal, and follow the steps for Setting a New Connection. When

prompted, select the COM port you will be using, then set the COM parameters (see Table 17 below):

5. To be sure that you have established a connection with your shaker, turn the shaker

OFF, open the HyperTerminal connection, then turn the shaker back ON. Once a

connection has been made, the shaker will send an OK to HyperTerminal.

6. You may wish to send a trial command to the shaker (see Sample Test below). We

suggest that you enable Caps Lock on your computer, because all commands must be

sent as capital letters.

Sample Test:

1. To request the current setpoint, type RS at the terminal. When you type R, you

should see the Innova 43/43R. When you type the S, you should see the echo S displayed at the computer.

2. Use the

cursor back over the first character.

RS is a Request command, so your shaker will send back the data corresponding to

the command.

PPPPEENNDDIIX

Table 17: COM Parameters for HyperTerminal

Baud Rate Data Bits

Parity Stop Bits Flow Control

R on the computer display. This is an echo character, sent back by the

 Enter key to send the carriage return control character. This will move the

Baud Rate. The default setting, as you see in your screen,

Enter the setting on your shaker (see Step 2 above) 8 None 1 None

EEMMOOTTEE

RROOGGRRAAMMMMIINNG

Mode parameter to Slave,

New Brunswick Operating manual

12.2 Overview of Command Sets

Using an optional RS-232 interface, the user has the capability to control various functions of the Innova 43/43R shakers by computer. A detailed command set follows this introduction.

The serial interface uses an echo character protocol that is implemented to indicate that a valid character has been received by the shaker. For every character sent by the user computer, the Innova 43/43R will return a matching character. This echo character should be read by the computer and compared to the character that was sent.

NOTE:

When you are sending a command, do not allow the pause between any two characters of your message to last longer than 10 seconds. If it takes any longer, the shaker’s serial port will reset itself.

In the following example, the Master (your computer) will command (the C in CS) the

shaker (Slave) to set its speed to 150 RPM:

Master sends: Innova 43/43R responds:

CS<Space>150<CR> CS<Space>150<CR>

In the next example, the Master (your computer) will request that the shaker (Slave) report (the R in RV) the actual parameters:

Master sends: Innova 43/43R responds:

RV<CR> First the echo: RV<CR>

Then the report: <Arg1><Tab><Arg2><Tab><Arg3>...<Arg7><CR><LF>

NOTE:

On power-up, even if the shaker is not set in shaker will always send these four characters:

Slave mode at the time, the

OK<CR><LF>. Before

starting any communications, be sure to clear the computer’s receive buffer.

This is the command set format:

Most Commands are defined as two capital characters using standard ASCII format (see the command sets provided below).

If a command has additional arguments, the ASCII space control character separates each argument. The command is terminated using the ASCII carriage return control character;

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

an optional line feed control character may also be included but must follow the carriage return character.

When a command is sent that returns data, the characters returned are in ASCII format. Each additional argument returned will be separated by the ASCII space or tab control character; refer to the selected command for the actual format. Data returned is terminated with a carriage return line feed control character sequence.

Section 12.3 provides an Index to the 2-character command codes.

Sections 12.4-12.7 provide command sets for your use for Set Commands, Profile Control Commands, Report Request Commands and Set/Get Date & Time.

12.3 Index to Command Codes

Command Code Meaning

CL Command Grow Lamp

CS Command Speed

CT Command Temperature

CU Command UV Lamp PC Clear Profile

PM Profile Mode PR Read Profile Step in Memory

PS Profile Stop/Start

PW Write Profile Step to Memory

RI Report Software Version RP Report Parameter List RS Report Setpoint Values RV Report Measured Values

=D Set Date and Time ?D Get Date and Time

New Brunswick Operating manual

12.4 Set Commands

Code Meaning Format

CL Command Grow

Lamp

CS Command Speed CT Command

Temperature

CU Command UV

Lamp

12.5 Profile Control Commands

Code Meaning Format

PC Clear Profile

(clears profile step)

PM Profile Mode

(returns status of current profile run/hold mode)

PC<Space><Arg1><Space><Arg2><CR> where Arg1= Profile number (range 1 to 4) and

Arg2=Step number (range 1 to 15) NOTE: If Arg2 is not included, all 15 steps of the

selected profile will be cleared. PM<CR> There are no parameters to this command.

Return data format: <Arg1><Space><Arg2><Space><Arg3><CR> where Arg1=Run/hold status (0 = end, 1 = run),

Arg2=Profile number and Arg3=Step number

NOTE: If a profile has not been selected to run, a 0

will be returned for Profile number and Step number.

...continued...

CL<Space><Arg1><CR> where Arg1= Set Grow Lamp state (0 = off, 1 = on) CS<Space><Arg1><CR> where Arg1= Agitation setpoint (####) CT<Space><Arg1><CR> where Arg1= Temperature setpoint (###.#) CU<Space><Arg1><CR> where Arg1= Set UV Lamp state (0 = off, 1 = on)

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Code Meaning Format

PR Read

Profile step in memory

PS Profile

Stop/ Start (stops or starts a program profile)

PW Write

Profile step to memory

PR<Space><Arg1><Space><Arg2><CR> where Arg1= Profile number (range 1 to 4) and Arg2=Step number (range 1 to 15)

Return data format: <Arg1><Space><Arg2><Space><Arg3><Space>...<CR><LF> where Arg1=Profile number (range 1 to 4),

Arg2=Step number, Arg3=Temperature setpoint, Arg4=Agitation setpoint, Arg5=(Reserved, reads 0.0), Arg6=Step hours, Arg7=Step minutes, Arg8=UV lamp, and Arg9=Grow lamp PS<CR>

With no other parameters, any profile currently running will be stopped.

PS<Space><Arg1><Space><Arg2><CR> where Arg1=Profile number (range 1 to 4), and

Arg2=Step number (range 1 to 15). NOTE: If Arg2 is not included, the profile will start in Step 1.

PW<Space><Arg1><Space><Arg2><Space><Arg3><Space>

...<CR> where Arg1=Profile number (range 1 to 4),

Arg2=Step number (range 1 to 15), Arg3=Temperature setpoint, Arg4=Agitation setpoint, Arg5=(Reserved); Enter 0.0, Arg6=Step hours (range 0 to 99), Arg7=Step minutes (range 0 to 59), Arg8=UV lamp (0 = off, 1 = on), and Arg9=Grow lamp (0 = off, 1 = on)

For example, the following PW command:

PW<Space>25.0<Space>150<Space>0.0<Space>2<Space>30<Space>0 <Space>1<CR>

will set Program 1, Step 1 to the following conditions:

 Temperature setpoint 25.0º  Step Time 2 hours 30 minutes  Agitation setpoint 150 rpm  UV Lamp OFF  GRO Lamp ON

New Brunswick Operating manual

12.6 Report Request Commands

Code Meaning Format

RI Report

Software Version

RP Report

Parameter List (ASCII text header)

Report Measured Values

Report Setpoint

Values

RI<CR> Return data format:

<Arg1><Tab><Arg2><CR><LF> where Arg1=Display module software version, and

Arg2=Control module software version RP<CR>

Return data format: <Arg1><Tab><Arg2><Tab><Arg3>...<Arg6><CR><LF> where Arg1=”Shaker Speed”,

Arg2=”Temperature”, Arg3=”% Relative Humidity”, Arg4=”Reserved”, Arg5=”Grow Lamp Status”, and Arg6=”UV Lamp Status” RV<CR> RS<CR>

Return data format: <Arg1><Tab><Arg2><Tab><Arg3>...<Arg6><CR><LF> where Arg1= Shaker Speed,

Arg2=Temperature, Arg3=% Relative Humidity, Arg4=(Reserved, reads 0.0), Arg5=Grow Lamp Status, and Arg6=UV Lamp Status

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

12.7 Set/Get Date & Time

Code Meaning Format

Set Date & Time

Get Date & Time

=D<Space><Arg1><Space><Arg2><Space><Arg3><Space>... <Arg7><CR>

?D<CR> Return data format: <Arg1><Tab><Arg2><Tab><Arg3><Tab>...<Arg7><CR><LF> where Arg1=Hours (range 0-23),

Arg2=Minutes (range 0-59), Arg3=Seconds (range 0-59), Arg4=Year (range 00-99), Arg5=Month (range 01-12), Arg6=Date (range 1-31), Arg7=Day (range 1-7; 1=Monday, 2=Tuesday...7=Sunday)

For example, this =D command:

=D<Space><01><Space><30><Space><00><Space><04><Space><07>

will set the real date and time on your shaker to:

1:30:00 am, 2004, July 4, Sunday.

New Brunswick Operating manual

Accessories, 63

Racks & Trays, 65 Actual Temperature, 47 Adjusting Lid Tension, 58 Alarms, 7, 18, 72 Arrows Icon, 17 ASCII Format, 76 ASCII Text Headers, 80 Audible Alarm

Disabling, 46 Audible Alarm Muted Icon, 16 Audible Alarms, 18 Automatic Restart, 49, 72

Baud Rate

Setting the, 39 Belt Replacement, 60 Biohazards, 50

Calibrate Screen, 40 Calibrating RPM, 48 Calspeed, 48 Chamber Light

Auto, 38

On/Off, 37 Changing a Setpoint, 35 Changing Baud Rate, 40 Changing Communication Mode, 39 Changing Screens, 34 Changing the Day, 36 Changing the Time, 37 Clamp Hardware Kits, 65 Clearance, 11 COM Parameters, 75 COMM Screen, 39

113

Command Codes, 76 Command Format, 76 Communication Mode, 40 Continuous Operation, 7 Control Knob, 16 Creating a Program, 42 Customer Satisfaction Form 6300, 9

Day, 36 Declaration of Conformity, 74 Decontamination, 50 Dimensions

Chamber, 72

Overall, 72 Display Icons, 17 Display Parameters, 16 Display Screen, 32

Changing Parameters, 32

Modifying Setpoints, 33 Display Screens

Changing the, 16 Door

Opening the, 31 Door Open Icon, 16, 17, 32 Drive, 72 Drive Belt

Removing the, 60

Replacing the, 60

Edit Program, 46 Electrical Requirements, 10, 72 Environment, 10

Firmware

Operation via, 7 Flask Capacities, 64

NNDDEEX

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

Flask Clamps, 64

Installation of, 22 Front View, 11 Fuses, 55

Gas Manifold Kit, 24, 66 Germicidal Lamp, 66 Glide-Up Door, 19 Growth

On/Off, 37 Growth Lamps, 66

Heater, 20, 72 Heater On Icon, 16, 17 HRS Alarm, 19 Humidity Monitor, 19, 66 HyperTerminal, 75

Icons, 17 Incomplete Order, 9 Indicated Temperature, 47 Inspection

Of Boxes, 9 Installing Flask Clamps, 22 Interior Light, 20

LAMP, 20 Lamps Screen, 37 LCD Display, 16, 72 Leveling

One Shaker, No Base, 13 Lid Tension

Adjusting the, 58 Load & Speed Graphs, 70 Locked Icon, 17 Locking Settings, 37 Loudspeaker Icon, 17, 37

Missing Parts, 9

Modifying a Setpoint, 33, 35 Monitor Mode, 40 Motor, 72 Moving Screen to Screen, 34 Mute, 37, 46 Mute Icon, 16, 17 Muting the Alarm, 37

Narrow Doorway, 11 New Program, 42

Operation Modes, 7 Overview, 7

Packing List

Verification of, 9 Padlock Icon, 17, 37 Parameter Locked Icon, 16 Photosynthetic Lamps, 20, 26, 27, 66 Platform

Available Types, 63 Platform Assemblies, 22 Platform Screw Locations, 22 Platforms, 8 POWER Alarm, 19 Power Failure, 49 Power Interruption, 49 Problems, 52 Product Returns, 54 Program Running Icon, 17 Programming, 42 Programs Screen, 41

Refrigeration, 20 Refrigeration Schematic, 70 Remote Alarm Option, 67 Remote Programming, 76 Replacement Parts, 63 Replacing UV Light, 59 Report Request Commands, 80 Resetting the Day, 36

New Brunswick Operating manual

Resetting the Time, 37 Returned Material Authorization Number,

54 Returned Material Safety Sheet, 54 RMA, 54 RS-232, 20, 72, 76 RS-232 Interface

Operation via, 7 RS232 Screen, 39 Run Icon, 16, 17, 46

Safety, 72 Safety Precautions, 31 Safety Switch, 7 Sample Program Screen, 45 Save Program, 45 Screen Parameters, 17 Service Accessibility, 21 Set Commands, 78 Setpoint Retention, 72 Setting the Baud Rate, 39 Setup Screen, 36

Changing the Day, 36

Changing the Time, 37

Locking Settings, 37

Muting the Alarm, 37 Shaking Speed, 72 Shipping Damage, 9 Slave Mode, 40 Software Interface, 20 Space Requirements, 11 Specifications, 72 SPEED Alarm, 19 Spill Pan, 19

Filling the, 29 Spills, 50

Summary Screen, 35

Changing Setpoints, 35

Symptoms, 52

Talk Mode, 40 TEMP Alarm, 19 Temperature Offset, 41, 47, 48

Setting the, 47 Temperature Offset Calibration, 47 Temperature Offset Value, 47 Temperature Range, 72 Test Tube Racks, 65 Time, 37 Timed Functions, 42 Timed Run, 7 Troubleshooting, 52

Unpacking

Tools for, 9 Utility Basket, 66 UV Decont

On/Off, 37 UV Lamp, 20, 66

Visible Alarms, 18

Warranty, 3 Water Reservoir, 19

Filling the, 29 Wavy Arrows Icon, 17

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

New Brunswick Operating manual

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Eppendorf 43R User Manual

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