Eppendorf 43R User Manual

2

COPYRIGHT:

Copyright © 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced
without the prior permission of the copyright owner.

The company reserves the right to change information in this document without notice. Updates
to information in this document reflect our commitment to continuing product development and
improvement.

TRADEMARKS:

Eppendorf® is a registered trademark, and New Brunswick™ and the New Brunswick
Logo™ are trademarks of Eppendorf AG, Hamburg, Germany.

Innova
USA.

Trademarks are not marked in all cases with ™ or ® in this manual.
Eppendorf has attempted to identify the ownership of all trademarks from public records. Any

omissions or errors are unintentional.

®

is a registered trademark of New Brunswick Scientific Co., Inc. of Enfield, Connecticut,

February 3, 2013
Revision F
M1320-0050

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

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CAUTION!

This equipment must be operated as described in this manual.
If operational guidelines are not followed, equipment damage
and personal injury can occur.

Please read the entire Operating manual before attempting to use

this shaker.

Do not use this equipment in a hazardous atmosphere or with
hazardous materials for which the equipment was not designed.

Eppendorf is not responsible for any
damage to this equipment that may result from the use of an
accessory not manufactured by Eppendorf.

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TABLE OF CONTENTS

1 OVERVIEW .......................................................................................................................... 7

2 INSPECTION & UNPACKING OF EQUIPMENT ......................................................... 9

2.1 INSPECTION OF BOXES ..................................................................................................... 9

2.2 PACKING LIST VERIFICATION .......................................................................................... 9

2.3 UNPACKING OF EQUIPMENT ............................................................................................. 9

2.4 OUT OF BOX CONCERNS .................................................................................................. 9

3 PREPARING THE LOCATION ....................................................................................... 10

3.1 PHYSICAL LOCATION ..................................................................................................... 10

3.2 ENVIRONMENT ............................................................................................................... 10

3.3 ELECTRICAL REQUIREMENTS ......................................................................................... 10

3.4 SPACE REQUIREMENTS .................................................................................................. 11

3.5 NARROW DOORWAYS .................................................................................................... 11

4 INSTALLATION ................................................................................................................ 13

4.1 LEVELING THE SHAKER ................................................................................................. 13

5 FEATURES ......................................................................................................................... 15

5.1 CONTROLS ..................................................................................................................... 15

5.2 LCD DISPLAY ............................................................................................................... 16

5.3 CHANGING SCREENS ...................................................................................................... 16

5.4 DISPLAY ICONS .............................................................................................................. 17

5.5 ALARMS ......................................................................................................................... 18

5.6 L

ID ................................................................................................................................. 19

5.7 SPILL PAN/WATER RESERVOIR ...................................................................................... 19

5.8 S

5.9 I

5.10 H

5.11 R

OFTWARE INTERFACES ................................................................................................. 20

NTERIOR LIGHT(S) ........................................................................................................ 20

EATER ......................................................................................................................... 20

EFRIGERATION (43R ONLY)......................................................................................... 20

5.12 SERVICE ACCESSIBILITY ................................................................................................ 21

6 GETTING STARTED ........................................................................................................ 22

6.1 PLATFORM ASSEMBLIES ................................................................................................ 22

6.2 INSTALLATION OF PLATFORM ........................................................................................ 22

6.3 I

NSTALLATION OF CLAMPS ............................................................................................ 22

6.4 ELECTRICAL CONNECTIONS ........................................................................................... 23

6.5 O

6.6 O

PTIONAL GASSING MANIFOLD KIT .............................................................................. 24

PTIONAL GERMICIDAL UV LAMP ................................................................................ 26

6.7 OPTIONAL PHOTOSYNTHETIC LAMPS ............................................................................. 26

6.8 O

PTIONAL HUMIDITY MONITOR .................................................................................... 27

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6.9 FILLING THE WATER RESERVOIR ................................................................................... 29

6.10 DRAINING THE WATER RESERVOIR ................................................................................ 30

7 OPERATION ...................................................................................................................... 31

7.1 SAFETY PRECAUTIONS ................................................................................................... 31

7.2 OPENING THE LID .......................................................................................................... 31

7.3 STARTING THE SHAKER .................................................................................................. 32

7.4 USING THE LCD SCREENS ............................................................................................. 32

7.4.1 Display Screen ...................................................................................................... 32

7.4.2 Summary Screen.................................................................................................... 35

7.4.3 Setup Screen .......................................................................................................... 36

7.4.4 Lamps Screen ........................................................................................................ 37

7.4.5 RS232 Screen ........................................................................................................ 39

7.4.6 Calibrate Screen ................................................................................................... 40

7.4.7 Programs Screen ................................................................................................... 41

7.5 PROGRAMMING THE SHAKER ......................................................................................... 42

7.5.1 Timer Only ............................................................................................................ 42

7.5.2 Programmed Steps ................................................................................................ 42

7.5.3 Creating a Program .............................................................................................. 42

7.5.4 Editing a Program ................................................................................................ 46

7.5.5 Running a Program............................................................................................... 46

7.6 MUTING THE AUDIBLE ALARM ...................................................................................... 46

7.7 TEMPERATURE OFFSET CALIBRATION ........................................................................... 47

7.7.1 Calculating the Offset Value ................................................................................. 47

7.7.2 Setting the Offset ................................................................................................... 47

7.8 USING CALSPEED ........................................................................................................... 48

7.9 POWER INTERRUPTION ................................................................................................... 49

8 MAINTENANCE ................................................................................................................ 50

8.1 ROUTINE MAINTENANCE ............................................................................................... 50

8.2 C

8.3 B

LEANING EXTERNAL & INTERNAL SURFACES .............................................................. 50

IOHAZARD DECONTAMINATION ................................................................................... 50

9 SERVICE & ACCESSORIES ........................................................................................... 52

9.1 T

9.2 P

9.3 O

ROUBLESHOOTING ....................................................................................................... 52

RODUCT RETURNS ........................................................................................................ 54

PENING THE SERVICE COMPARTMENT ......................................................................... 55

9.4 FUSE REPLACEMENT ...................................................................................................... 55

9.5 R

EPLACING CABINET LIGHTS ......................................................................................... 56

9.6 REPLACING OPTIONAL PHOTOSYNTHETIC LAMPS .......................................................... 57

9.7 A

9.8 R

9.9 B

DJUSTING LID TENSION ............................................................................................... 58

EPLACING OPTIONAL GERMICIDAL LAMP .................................................................... 59

ELT REPLACEMENT OR ADJUSTMENT .......................................................................... 60

9.10 REPLACEMENT PARTS .................................................................................................... 63

9.11 A

CCESSORIES ................................................................................................................. 63

9.11.1 Platforms ............................................................................................................... 63

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9.11.2 Flask Clamps for Platforms .................................................................................. 64

9.11.3 Replacement Clamp Hardware Kits ..................................................................... 65

9.11.4 Test Tube Racks & Other Accessories .................................................................. 65

9.11.5 Optional Gas Manifold Kit ................................................................................... 66

9.11.6 Optional Photosynthetic Lamps ............................................................................ 66

9.11.7 Optional UV Germicidal Lamp ............................................................................. 66

9.11.8 Optional Humidity Monitor .................................................................................. 66

9.11.9 Optional Utility Basket ......................................................................................... 66

9.11.10 Optional Remote Alarm .................................................................................... 67

10 DRAWINGS, GRAPHS & TABLES ............................................................................ 68

10.1 SCHEMATICS .................................................................................................................. 68

10.2 LOAD & SPEED GRAPHS................................................................................................. 70

11 SPECIFICATIONS ......................................................................................................... 72

11.1 CERTIFICATIONS ............................................................................................................ 73

11.1.1 CE Declaration of Conformity .............................................................................. 74

12 APPENDIX: REMOTE PROGRAMMING ............................................................... 75

12.1 SETTING UP WITH HYPERTERMINAL .............................................................................. 75

12.2 OVERVIEW OF COMMAND SETS ..................................................................................... 76

12.3 INDEX TO COMMAND CODES ......................................................................................... 77

12.4 SET COMMANDS ............................................................................................................ 78

12.5 PROFILE CONTROL COMMANDS ..................................................................................... 78

12.6 REPORT REQUEST COMMANDS ...................................................................................... 80

12.7 SET/GET DATE & TIME .................................................................................................. 81

13 INDEX .............................................................................................................................. 82

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

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1

1

The Innova 43/43R Incubator Shakers are large-capacity orbital shakers that utilize an eccentric
counterbalanced drive mechanism. They provide horizontal plane rotary motion in either a
1-inch (2.54 cm) or a 2-inch (5 cm) diameter circular orbit, depending on the model. A
Proportional/Integral (PI) microprocessor controller with instantaneous digital feedback controls
the speed over the entire speed range.

The Innova 43R provides temperature control from 20º below ambient (as low as 4ºC) to 80C,
and the Innova 43 from 5º above ambient to 80ºC. Naturally, both these ranges depend on
relative humidity and other ambient factors, as well as the options installed in the unit. Ambient
temperature is measured at one meter from the exterior of the unit.

Erlenmeyer flasks (up to 6 liters in size) as well as a wide variety of tubes and plates can be
accommodated using the New Brunswick shaker accessories described in Section 9.11. These
are easily accessed on the platform.

To facilitate moving the unit, each shaker is equipped with casters that roll freely when the feet
are raised.

The Innova 43/43R may be operated in the following ways:

 Continuously: at a set speed and temperature, until user intervention.
 In a timed mode: run at a set speed, time and temperature for a period of up to 99.9

hours, after which the shaker automatically shuts off.

 Via the shaker’s programmable controller: run through multiple temperature and

speed changes for an extended period of time.

 Via computer through an RS-232 interface.

See Section 7 for more details on these various modes of operation.

For safe operation, the Innova 43/43R shakers are designed with a safety switch that
automatically stops the shaker mechanism when the lid is opened.

The Innova 43/43R is equipped with visual and/or audible alarms that alert the user to the
following conditions:

 The end of a timed run
 Deviations from speed setpoint
 Deviations from temperature setpoint
 Power failure
 Lid open

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To accommodate customer needs, a wide variety of platforms can be used with the Innova
43/43R:

 Universal platforms are the most flexible, providing hole patterns for flask clamps,

test tube racks and other accessories.

 Dedicated platforms are supplied with flask clamps attached; they are designed solely

and expressly for this purpose.

 Test tube racks, microplate holders, and test tube rack holders are also available (a

universal platform is needed for all test tube racks and holders).

For further information on these accessories, see Section 9.11.

We also recommend that you consult the load and speed graphs provided in Section 10.2.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

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2.1 Inspection of Boxes

After you receive your order from Eppendorf, inspect the boxes carefully for any damage
that may have occurred during shipping. Report any damage immediately to the carrier
and to your local Eppendorf Customer Service Department.

2.2 Packing List Verification

Verify against your packing list that you have received all of the correct materials.

2.3 Unpacking of Equipment

CRUSH WARNING!

Do not attempt to lift the Innova 43/43R by hand.
Always use a lifter or other suitable equipment when raising or handling
the unit.

To unpack your Innova 43/43R, you will need the following tools:

 Claw hammer
 Scissors to cut nylon strapping
 Tool to remove 3-inch (7.6 cm) metal staples

Save all packing materials and this user’s guide/operating manual.

T

2.4 Out of Box Concerns

If any part of your order was damaged during shipping, is missing pieces, or fails to

operate properly, please fill out the Customer Satisfaction Form 6300, included with your

equipment, and return it by fax.

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3

3

3.1 Physical Location

It is essential that the instrument be situated in an area where there is sufficient space for
the shaker to clear walls and potential obstructions during operation. The surface on
which the unit is placed must be smooth, level, and able to support the shaker under full
load operating conditions.

CRUSH WARNING!

Do not attempt to lift the Innova 43/43R by hand.
Always use a lifter or other suitable equipment when raising or handling
the shaker.

NOTE:

The Innova 43/43R has casters and leveling feet. When you need to move
the shaker across the floor, make sure the feet are raised enough so that the
casters can roll freely without scraping the floor with the feet. At all other
times, make sure the feet are down, to avoid any unintended rolling.

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3.2 Environment

The shaker is designed to operate optimally in the following ambient conditions:

 10º to 35°C
 20 to 80% Relative Humidity (non-condensing)

3.3 Electrical Requirements

The Innova 43/43R can be equipped to run on:

 100 Volts, 50 or 60 Hz, 1500 VA maximum
 120 Volts, 60 Hz, 1500 VA maximum
 230 Volts, 50 Hz, 1500 VA maximum

Check your shaker’s Electrical Specification Plate (located on the back of the shaker) to
be sure the electrical requirements of your shaker match the output of your electric
supply. If they do not match, contact your Eppendorf representative.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

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3.4 Space Requirements

Allow at least four inches (10 cm) around the shaker for proper ventilation and for access
to Power Switch and RS-232 port accessibility on the right side. Also allow enough

room above the shaker for the lid to be fully open (see Figure 1).

Be sure to keep the power plug and power outlet easily accessible to facilitate unplugging
the shaker, as needed.

Figure 1: Innova 43/43R Front View

1

5

2

3

4

1 Depth: 32 inches (81 cm) including front panel protrusions 4 Pedal to open lid
2 Width: 45.5 inches (115.6 cm) 5 Handle to open lid
3 Height: 40.6 inches (103 cm) with feet

NOTE: Open lid adds 23.5 inches (59.7 cm)

NOTE:

Be sure to allow at least four inches (10.5 cm) around shaker for ventilation,
access to power cord (rear panel), and access to power switch and RS-232
port (right side).

3.5 Narrow Doorways

If you need to move the Innova 43/43R through a doorway that is less than 32.5 inches
(82.5 cm) wide, remove the front window cover to reduce the depth of the shaker to 30.3
inches (77 cm):

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1. Carefully remove the five screws on the underside of the plastic window cover,

retaining them for reuse.

2. Being very careful not to hit the control knob or button, slide the window cover

upward to remove it from the spring clips on the front of the shaker.

When the shaker is in the desired location, reinstall the window cover by reversing the
procedure.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

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4

4

CRUSH WARNING!

Do not attempt to lift the Innova 43/43R by hand.
Always use a lifter or other suitable equilment when raising or handling
the shaker.

4.1 Leveling the Shaker

NOTE:

The Innova 43/43R has casters and leveling feet. When you need to move
the shaker across the floor, make sure the feet are raised enough so that the
casters can roll freely without scraping the floor with the feet. At all other
times, make sure the feet are down, to avoid any unintended rolling.

Make sure that the shaker is placed on a level surface, in its intended location.
Lower all four adjustable feet as described below until they are solidly on the surface. If
the shaker is not level, adjust the feet as needed to achieve leveling:

1. Immobilize the top lock nut against the shaker with one wrench whenever you

adjust the foot, to keep the threaded stud from falling out (see Figure 2).

Figure 2: Adjustable Foot (as shipped)

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3

4

5

1 Bottom of shaker 3 1.75 inch (4.45 cm) 5 Foot
2 Lock nut 4 Flats for wrench

2. With a second wrench against the flats of the threaded stud, just above the foot.

Rotate clockwise to lower the foot or counter-clockwise to raise the foot.

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3. Place a level on the top of the shaker. If necessary, make further adjustments by

repeating all steps until the shaker is level.

4. After installing a platform (see Section 6), fully load the shaker and do a test run at

normal speed (see Section 7). Make additional leveling adjustments if necessary.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

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5.1 Controls

1

1 Shaking Start/Stop switch 3 RS-232 port 5 Control knob
2 Display 4 Power switch

 START/STOP SWITCH

 CONTROL KNOB

 RS-232 PORT



POWER SWITCH

5

5

Figure 3: Front Panel (detail)

2

This switch is used to start or stop the shaker. It will also
activate the timer when a timed run is desired. If the
shaker is stopped and restarted, the timer automatically
returns to the beginning of a run.

This knob is multifunctional. It is used to change
screens, and to select and change operating conditions.

See Section 5.8 for details.

This rocker switch is a circuit breaker that turns power on
and off to the entire Innova 43/43R.

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NOTE:

In addition to the power switch, the power cord is also used to conduct
power or to break the power circuit to the shaker. Whenever power to the
shaker may be a hazard (during cleaning, maintenance or service work), be
sure to disconnect the power cord from the electrical outlet.

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5.2 LCD Display

When the shaker is powered up, using the Power Switch located on the right side of the

machine (see Figure 3), the initial display screen will remain for a short time while the

system boots up. Then the main screen, called
This screen will indicate the same parameters that were in effect when the power was
turned off.

DISP for Display, appears (see Figure 4).

Figure 4: Display Screen

2

1

ºC 23

1

RPM 120

10

 For an explanation of the icons on the display screen, see Section 5.4.
 Turning the Control Knob will highlight functions and/or values that can be changed.
 Whenever you turn the Control Knob, the chamber light will go on (for details, see

For more information on working in the Display Screen, see Section 7.4.1.

DISP We 13 : 44

9

1 Parameters 5 Program running
2 Temperature offset 6 Heater on
3 Actual parameter values 7 Lid open
4 Day & 24-hour time 8 Parameters locked
9 Audible alarm muted 10 Screen name

Section 5.9).

8

7

6

5

4

3

3

5.3 Changing Screens

You can change screens displayed by highlighting the screen name field in the lower left
corner, pressing the Control Knob in until it clicks, rotating the Knob left or right (which
also makes clicking sounds) to the desired screen and clicking the Control Knob in again.
Table 1 describes the various screens:

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

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Table 1: Screens

Screen

Meaning Features/Modes

Name

DISP Display Shows two user-selectable parameters* and actual values.

SUMM Summary Shows all parameters*, setpoints and actual values.

SET Set-Up Set day of week, set time, enable or mute alarm, lock or

unlock operating parameters

LAMP Lamps

COMM Communication

(RS-232)

CAL Calibration Allows user to enter a temperature offset.

PROG Program Allows user to set up 1- 4 programs, each with 1-15 steps.

* see Table 2, Display Screen Parameters  Optional Not installed

Internal chamber light: ON (always on); OFF (always
off); AUTO (default mode), light goes on and stays on
when lid is open, shuts off 15 seconds after lid is closed,
and goes on for 15 seconds when Control Knob is moved.

Photosynthetic lights (GRO): ON, OFF,
UV Light (UV): ON, OFF,

SET: set baud rate
OFF disables RS-232
MONITOR: PC commands shaker to read setpoints and
actual values on a schedule determined by PC software.
Parameters are unlocked and can be changed by program
or manually.
SLAVE: PC controls shaker and logs data.
TALK: Shaker sends setpoint and actual data to PC at
one-minute intervals.

Self-calibrates the speed sensor.



NONE



NONE

Table 2: Display Screen Parameters

Parameter Name Meaning

RPM Shaking speed, in revolutions/minute

ºC Chamber temperature, in degrees Celsius

HRS Programmed time remaining, in hours

 %RH Relative Humidity, in percent.
 UV Status of Ultraviolet germicidal lamp
 GRO Status of Photosynthetic growth lamps

 Optional

5.4 Display Icons

There are six icons that help identify operating conditions. Five of these icons are
located at the bottom of the display, and they are visible, when applicable, in any screen
you are viewing, except the Program subscreens. The sixth appears, when applicable,
next to ºC whenever the temperature is onscreen.

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Table 3: Display Icons

Icon Explanation

This icon appears when audible alarms are muted.

This icon appears when the possibility to make manual or
programmed parameter changes is disabled (locked). This is
controlled by settings on the SET screen. See Section 7.4.3

This icon appears when the shaker lid is open.

This icon appears when the Heater is on. See Section 5.10.

This icon appears when a user-defined Program is running. See
Section 7.5.5.

This icon appears to the right of ºC if the Temperature Offset
feature is being used. See Section 7.7.

5.5 Alarms

If an alarm condition exists, the field in the lower right corner will alternate the Day and
Time with characters indicating the nature of the alarm condition, accompanied by an
audible alarm (unless muted):

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

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Table 4: Alarms

Indication Description

TEMP

SPEED

POWER

HRS Indicates when timed run is completed.

The temperature deviates more than 1ºC from setpoint after
achieving control temperature range. After lid is opened,
alarm will be disabled for 5 minutes while chamber recovers
to setpoint.
The speed deviates more than 5 RPM from setpoint after
achieving operating speed setpoint. After lid is opened,
alarm will be disabled for 5 minutes while chamber recovers
to setpoint.
Indicates shaker is powering up (both at normal power-up
and after power interruption); will flash until the Control Knob
is moved.

5.6 Lid

Access to the chamber of the Innova 43/43R is through the top-opening hinged lid. To

open the lid, firmly press the pedal or lift up on the front handle (see Figure 1). The lid

stands on its hinges until you choose to close it.

When the lid opens, the following will happen:

 Heater turns off
 Shaker stops
 Interior light goes on and, when it is in AUTO mode, will remain on for 15

seconds after the lid is closed

 UV germicidal lamp (if so equipped) turns off

5.7 Spill Pan/Water Reservoir

The Innova 43/43R is equipped with a spill pan and spill cover to protect the drive
mechanism in the case of accidental spills and/or broken glassware. This pan can also be
used as a water reservoir to humidify the chamber and to reduce evaporation. An
optional factory-installed humidity monitor is also available.

The reservoir can be drained through the quick-connect valve on the front of the shaker.

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5.8 Software Interfaces

The RS-232 port is next to the Power Switch on the right side of the control panel (see
Figure 3). It can be used to interface a computer to the shaker for control of operating

conditions or data logging applications (see Section 12).

The customer is responsible for securing the proper driver to interface with the RS-232.

5.9 Interior Light(s)

When the

LAMP screen is in its default AUTO mode, the interior (“chamber”) light is

activated for 15 seconds whenever you turn the Control Knob. It will automatically shut
off after 15 seconds of Control Knob inactivity.

The chamber light will also go on when the lid is open.

In addition, you can set the chamber light to be continuously
either mode in the LAMP screen.

There are two additional light options for refrigerated shakers only: interior

photosynthetic growth lamps (see Section 6.7) and a germicidal UV lamp located outside
the chamber but in the airflow path (see Section 6.6).

ON or OFF by selecting

5.10 Heater

The chamber temperature is sensed by a 1000 ohm platinum RTD. A 650W heater is
controlled using pulse width modulation on a 2.5-second duty cycle. This cycle time is
fast enough to prevent noticeable changes in air temperature due to the cycling.

Whenever the heater is on, the Heater On icon

will appear in the display. The

heater automatically stops running when the lid is opened.

NOTE:

Operating the shaker for sustained periods at temperatures above 60°C may
result in a shorter fan lifespan.

5.11 Refrigeration (43R Only)

The refrigeration system in the Innova 43R is a variable-capacity system carefully
designed with self-checks to maintain the setpoint, to balance pressure within the system,
and to prevent freezing on the evaporator surface.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

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When the shaker is powered up, there is a four-minute time delay prior to compressor
start-up.

5.12 Service Accessibility

In the unlikely event that your Innova 43/43R should need service, all electronic boards,

refrigeration, heating components are easily accessed, by an authorized service
technician, behind the right side panel of the shaker.

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6.1 Platform Assemblies

The Innova 43/43R can be used with a variety of New Brunswick platforms that will

accept a wide range of clamps for flasks, test tubes, etc. A platform, which is required
for operation, is a separate item, not included with the shaker assembly. Refer to

Section 9.11 for details on available platforms and platform accessories.

6.2 Installation of Platform

Prior to use, a spill cover and platform must be installed on the shaker. The shaker
is shipped with four Allen head platform screws installed in the bearing housing (see
Figure 5 below, which also shows the spill cover that you must install).

Figure 5: Installing Platform & Spill Cover

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1 Platform screws 3 Spill cover
2 Platform 4 Bearing housing

6.3 Installation of Clamps

Flask clamps purchased for use with universal platforms (see Section 9.11.2) require

installation. Clamps are installed by securing the base of the clamp to the platform with
the correct type and number of screws. All clamps are shipped complete with hardware.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

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2

Clamps for 2-, 2.8-, 4- and 6-liter flasks are shipped with an additional girdle to keep the

flasks in place (see Figure 6). To install these double girdle clamps:

1. Place clamp on platform, secure in place with correct type of screws: #S2116-3051,

10-24 x 5/16-inch flat Phillips head, provided with the clamps (see Figure 6a).

2. Place the loose girdle around the upper portion of clamp body so that it is held in

place by the legs of the clamp.

3. Insert the flask into the clamp, and push the girdle down so the rubber tubes are in

contact with the platform and the flask.

Figure 6: Double Girdle Clamp Installation

3

1

1 Platform 4 Clamp body (legs & base)
2 Clamp mounting holes (5) 5 Lower girdle with girdle tubes
3 Upper girdle with girdle tubes

Figure 6a: Clamp Fastener

6.4 Electrical Connections

Before making electrical connections, verify that the power source voltage matches the
voltage on the electrical specification plate and that the
position. The electrical specification plate is located on the rear panel of the shaker near
the power connector.

5

4



ON/OFF switch is in the OFF

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Connect the power cord to the power connector, then connect the other end to a suitable,
grounded receptacle. Make sure there is enough clearance to disconnect the plug
whenever necessary.

Figure 7: Rear Panel

1

2

3

1 Power connector
2 Exhaust vents: do not block!!
3 Adjustable foot

6.5 Optional Gassing Manifold Kit

This option is factory-installed. The manifold delivers gas into the chamber via as
many as 12 ports (see Figures 8 & 9 on the following page). The manifold can be

adapted to the desired tubing configuration by adding or subtracting ports or by
temporarily clamping off unused tubes. You may elect to use splitters (barbed Y­connectors) after the manifold to increase the number of flasks you can serve.

You determine the appropriate gas flow rate using a pressure regulator (which you
supply) on the gas supply.

NOTE:

Gas supply should be regulated and it must never exceed 15 PSIG.

50 feet of 1/16-inch (ID) sterilizable silicone tubing is supplied with the kit. Filters may
be needed: 0.22μ syringe filters (which you supply) can be fitted to the individual
manifold ports to maintain a sterile barrier.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual

25

Figure 8: Gas Monitor (optional)

Figure 9: Gas Fitting (with optional Gas Manifold)

1

1 Gas manifold connection (1/4 FNPT)

SAFETY PRECAUTIONS!

 Never use the gas manifold with flammable gases.
 Never exceed 15 PSIG inlet pressure to the manifold.
 Make sure free lengths of tubing are secured by the hook and loop straps
to keep them from contact with flasks or other moving parts.

New Brunswick Operating manual

26

6.6 Optional Germicidal UV Lamp

WARNING!

Never try to operate the UV Germicidal Lamp while the shaker lid is open.

This option, a germicidal ultraviolet lamp, is placed inside the Service Compartment (see
Figure 10), outside the chamber to help reduce the risk of contamination. The lamp is

identified on the display screen as UV.

Figure 10: Germicidal UV Lamps (optional)

1

1 Front of shaker

The UV germicidal lamp is factory-installed and available on refrigerated shakers only.
Operation instructions are provided with this option (see Section 9.11.7).

6.7 Optional Photosynthetic Lamps

This factory-installed option, available on refrigerated shakers only, provides nine

photosynthetic growth lamps inside the chamber (see Figure 11 on the following page).

They can be turned on and off manually, by the easily set programmable timer, or by
computer. These lamps are identified on the display screen as GRO. The replacement
bulb part number is P0300-0221.

Innova 43 & 43R Incubator Shakers  M1320-0050 Operating manual