Bio-Rad Plasmid Mini Purification Module User Manual

Bio-Rad Laboratories, Inc.

2000 Alfred Nobel Dr.
Hercules, CA 94547 USA
[phone]

1-800-424-6723

4110111 Rev C

Aurum

™

Plasmid Mini Kit

Instruction Manual

For technical service, call your local Bio-Rad office, or
in the US, call 1-800-424-6723

Table of Contents

Section 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Section 2 Kit Components . . . . . . . . . . . . . . . . . . . . . . . . .1

Section 3 Storage Conditions . . . . . . . . . . . . . . . . . . . . . . .2

Section 4 Necessary Supplies . . . . . . . . . . . . . . . . . . . . . .2

Section 5 Guidelines for Using the

Aurum Plasmid Mini Kit . . . . . . . . . . . . . . . . . . . . .3

Section 6 Protocol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5

Section 7 Troubleshooting Guide . . . . . . . . . . . . . . . . . . .11

Section 8 Ordering Information . . . . . . . . . . . . . . . . . . . .13

Section 1
Introduction

The new Aurum plasmid mini kit is optimized for the purification of up to 20 µg
of plasmid DNA, rapidly and inexpensively, without the use of toxic reagents or
alcohol precipitations. The use of membranes to bind and purify plasmid DNA,
in combination with an optimized column design, minimizes handling and
allows plasmid purification to be carried out in either a vacuum or spin format.
In either format, the final elution is carried out in a microcentrifuge. Plasmid
DNA purified with the Aurum plasmid mini kit is eluted into a small volume of
aqueous buffer and is free of salts, bacterial chromosomal DNA, and RNA.
The exceptional purity of the plasmid produced by this system makes it ideal
for use in automated fluorescent sequencing and in any other molecular
biology application.

Section 2
Kit Components

The Aurum plasmid mini kit contains the following components:

resuspension solution 25 ml

lysis solution 25 ml

neutralization solution 40 ml

wash solution 25 ml (5x concentrate)

elution solution 16 ml

plasmid mini columns 100

2 ml capless wash tubes 100

The solutions are specifically formulated for the Aurum plasmid mini kit. They
are NOT interchangeable with solutions used in other kits or protocols.

1

Section 3
Storage Conditions

Solutions and columns should be stored at room temperature. If
precipitation is observed in any solution, warm solution to 37ºC to
redissolve, and allow to return to room temperature before use. Do not
expose any of the solutions to temperatures above 37ºC. If the kit is
used infrequently, storage of the resuspension solution at 4ºC is
recommended to preserve the RNase.

Section 4
Necessary Supplies

Equipment and supplies to be provided by the customer:

• 1.5–2.0 ml capped microcentrifuge tubes 200

• 95–100% ethanol, or reagent-grade (denatured) ethanol, 100 ml

• Microcentrifuge (12,000 x g)

Additional equipment required for vacuum format:

• Bio-Rad Aurum vacuum manifold with vacuum regulator and
column adaptor plate (Cat. #732-6470), or other vacuum manifold
with luer fittings (Figure 1).

Note: Please read Section 7, Instrument Setup and Use for the
Column Adaptor Plate in the Aurum vacuum manifold instruction

manual for proper vacuum setup conditions.

• Vacuum source (capability of -20 to -23" Hg required)

2