550 Ultramark
Ultramark™ Microplate Reader
User Manual
410-0164 Rev B
Ultramark Catalog Number 170-9500
Ultramark User Manual Catalog Number 170-9502
Bio-Rad Technical Services Department
Open Monday–Friday, 8:00 a.m. to 4:00 p.m., Pacific Standard Time.
Phone: (800) 424-6723, option 2, option 3
(510) 741-6576
Fax: (510) 741-5802
E-mail: LSG.TechServ.US@Bio-Rad.com (U.S.)
LSG.TechServ.Intl@Bio-Rad.com (International)
Notice:
No part of this publication may be reproduced or transmitted in any form or by any means, electronic
or mechanical, including photocopy, recording, or any information storage or retrieval system,
without permission in writing from Bio-Rad.
Ultramark is a trademark of Bio-Rad Laboratories. The Bio-Rad logo is a registered trademark of
Bio-Rad Laboratories. All other trademarks and registered trademarks are of their respective
companies.
Warranty and Regulatory Notices
Warranty Statement
This warranty may vary outside of the continental United States. Contact your local Bio-Rad office for
the exact terms of your warranty.
Bio-Rad Laboratories warrants that the Ultramark system will be free from defeats in material and
workmanship, and will meet all performance specifications for the period of 1 year from the date of
shipment. This warranty covers all parts and labor.
In the event that the instrument must be returned to the factory for repair under warranty, the
instrument must be packed for return in the original packaging.
Bio-Rad shall not be liable for any incidental, special, or consequential loss, damage, or expense
directly or indirectly arising from the use of the Ultramark system. Bio-Rad makes no warranty
whatsoever in regards to products or parts furnished by third parties, such being subject to the
warranty of their respective manufacturers. Service under this warranty shall be requested by the
contacting your nearest Bio-Rad office.
This warranty does not extend to any instruments or parts thereof that have been subject to misuse,
neglect, or accident, or that have been modified by anyone other than Bio-Rad or that have been used
in violation of Bio-Rad instruction.
The foregoing obligations are in lieu of all other obligation and liabilities including negligence and all
warranties, of merchantability, fitness for a particular purpose or otherwise, expressed or implied in
fact or by law, and state Bio-Rad’s entire and exclusive remedy for any claims or damages in
connection with the furnishing of goods or parts, their design, suitability for use, installation, or
operation. Bio-Rad will in no event be liable for any special, incidental, or consequential damages
whatsoever, and Bio-Rad’s liability under no circumstances will exceed the contract price for the
goods for which liability is claimed.
Regulatory Notices
Important: This Bio-Rad instrument is designed and certified to meet EN55011, EN50082-1, and IEC
1010-1 requirements, which are internationally accepted electrical safety standards. Certified
products are safe to use when operated in accordance with the instruction manual. This instrument
should not be modified or altered in any way. Alteration will:
• Void the manufacture’s warranty.
• Void the regulatory certification.
• Create a potential safety hazard.
Note: This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to Part 15 of the FCC rules. These limits are designed to provide reasonable
protection against harmful interference when the equipment is operated in a commercial
environment. This equipment generates, uses, and can radiate radio frequency energy and, if not
installed and used in accordance with the instruction manual, may cause harmful interference to
radio communications. Operation of this equipment in a residential area is likely to cause harmful
interference in which case the user will be required to correct the interference at his/her own
expense.
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Table of Contents
1. General Information..................................................................................................1
1.1 Introduction........................................................................................................................ 1
1.2 Ultramark Specifications....................................................................................................1
2. Equipment Set-up and Installation..........................................................................3
2.1 Unpacking the Ultramark Microplate Reader .................................................................... 3
2.2 SCSI II Cable Connection ................................................................................................. 3
2.3 Power Connection ............................................................................................................. 3
2.4 Location of the Instrument................................................................................................. 4
3. Using the Ultramark..................................................................................................5
3.1 Loading a Plate.................................................................................................................. 5
3.2 Reading a Plate................................................................................................................. 5
3.3 Incubator Controls ............................................................................................................. 5
4. Changing Filters in the Ultramark ...........................................................................7
5. Recovering from a Filter Error.................................................................................9
6. Recovering a Dropped Filter..................................................................................11
7. Disinfection Procedure ..........................................................................................13
8. Error Codes .............................................................................................................15
9. Product Information ...............................................................................................19
Order Form—Emission Filter .......................................................................................21
Disinfection Certificate .................................................................................................23
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1. General Information
1.1 Introduction
The Ultramark microplate reader provides all the flexibility you need for virtually any absorbance
assay. You can use it to perform standard colormetric ELISA and kinetic readings for general cell
screening with 6, 12, 24, 96, 384, and 1536-well microplate formats. The Ultramark has a 10-magazine
filter holder with a working wavelength range of 260–750 nm and an incubation temperature range
up to 42 degrees Celsius. The optical design provides verifiable results up to 4.5 O.D. with an optical
resolution 500 microns.
1.2 Ultramark Specifications
Light Source Xenon Flash Lamp
Spectral Range 250–750 nm
Filter Capacity 10-position magazine
Shaking Yes
Incubation
Power 100–240 V, 60/50 Hz, 22 Amps
Dimensions 20 cm (H) x 43 cm (W) x 51 cm (D)
Compatible Plates Any microplate or suitable medium with dimensions 138 mm
Resolution 500 microns
Linearity Less than 0.5% CV from 0–4.0 O.D. at 405 nm
Precision Less than 0.5% CV from 0–4.0 O.D. at 405 nm
Software Compatibility A System 486 or higher, 32 Mb RAM, Windows 95 OS or higher
Read Times 96-well plate: 6 seconds single wavelength
Compatible Plate types Any format from 1 well to 1536 wells, up to a plate height of 15
Environmental Operating
Range
Up to 42°C , programmable in 0.2° C increments
(length) x 98 mm (width). Plate height cannot exceed 15 mm.
24 seconds dual wavelength
384-well plate: 12 seconds single wavelength
32 seconds dual wavelength
1536-well plate: 34 seconds single wavelength
78 seconds dual wavelength
mm.
5–40 degrees C; max. relative humidity 80% non-condensing
1
2. Equipment Set-up and Installation
2.1 Unpacking the Ultramark Microplate Reader
Before opening the box, check for any outer damage. Document any damages, if present.
Place the box upright with the TOP label facing up and open the box. Remove the instrument from
the box in this position only.
Warning!
it.
Check the instrument for damaged or broken parts. Document any damages in detail.
Each Ultramark instrument includes the following accessories. Verify that they are present:
• 1 power cord.
• 1 plastic container with filter holder and 10 filters: 260 nm, 280 nm, 340 nm, 405 nm, 415 nm,
• 1 user manual.
Verify that the serial number on the back of the instrument is the same as that on the outside of the
box.
Remove the side cover on the right side of the unit. Inside there are two docking brackets that need to
be removed by unscrewing 3 screws as shown on the unpacking sheet attached to the unit. After
removing the two docking brackets, install the Filter Magazine as described in the unpacking
instructions.
Grasp the instrument firmly under the right and left sides before attempting to lift or carry
490 nm, 550 nm, 630 nm, 655 nm, and 750 nm.
2.2 SCSI II Cable Connection
The SCSI II port is located on the back of the instrument. There are two connectors, which allows the
user to connect more than one SCSI instrument in a chain. If the Ultramark is the last instrument in
the chain, make sure that the termination switch (next to the SCSI connectors) is in the “ON” position.
If the Ultramark is an intermediate unit in the chain, make sure that the termination switch is in the
“OFF” position. If the Ultramark is the only unit connected to the SCSI cable, make sure that the
switch is in the “ON” position.
There is a small dial next to the SCSI connectors on the back of the unit. This dial selects the SCSI ID
number for the unit at boot-up. Be sure to select an ID number that is not being used by any other
SCSI device in the chain.
2.3 Power Connection
Before switching the power on for the first time, allow the Ultramark to warm to room temperature
for 6 hours after unpacking to prevent any condensation problems.
Plug the included power cord into the main power connector port. Make sure that the instrument is
connected to a grounded conductor of the main power outlet.
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2.4 Location of the Instrument
The Ultramark microplate reader is a precise optical measuring instrument. The following conditions
must be met to ensure maximum sensitivity:
• Place the instrument on a flat surface that is free of vibration.
• Do not place the instrument in direct sunlight.
• Make sure that the instrument is being operated in a dust-free environment.
Warning!
attached to ensure proper data sampling and protect users from moving parts.
Do not operate the instrument with the filter cover off. Always have the filter cover
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