Bio-Rad 422 Electro-Eluter User Manual
Model 422
Electro-Eluter
Instruction
Manual
Catalog Numbers
165-2976 and 165-2977
For Technical Service
Call Your Local Bio-Rad Office or
in the U.S. Call 1-800-4BIORAD
(1-800-424-6723)
Note
To insure best performance from the Model 422 Electro-Eluter, become fully acquainted with these operating instructions before using the cell to separate samples. Bio-Rad recommends that you first read these instructions carefully. Then assemble and disassemble the
cell completely without casting a gel. After these preliminary steps, you should be ready to cast
and run a gel.
Bio-Rad also recommends that all Model 422 Electro-Eluter components and accessories
be cleaned with a suitable laboratory cleaner (such as Bio-Rad Cleaning Concentrate, catalog
number 161-0722) and rinsed thoroughly with distilled water, before use.
Warranty
Bio-Rad Laboratories warrants the Model 422 Electro-Eluter against defects in materials
and workmanship for 1 year. If any defects occur in the instrument during this warranty period, Bio-Rad Laboratories will repair or replace the defective parts free. The following defects,
however, are specifically excluded:
1. Defects caused by improper operation.
2. Repair or modification done by anyone other than Bio-Rad Laboratories or an authorized
agent.
3. Use of fittings or other spare parts supplied by anyone other than Bio-Rad Laboratories.
4. Damage caused by accident or misuse.
5. Damage caused by disaster.
6. Corrosion due to use of improper solvent or sample.
For any inquiry or request for repair service, contact Bio-Rad Laboratories after confirming the model and serial number of your instrument.
Model
Catalog No.
Date of Delivery
Warranty Period
Serial No.
Invoice No.
Purchase Order No.
Table of Contents
Section 1 Introduction..................................................................................................1
1.1 Specifications.............................................................................................................1
Section 2 Equipment and Reagents............................................................................2
2.1 Equipment..................................................................................................................2
2.2 Modular Mini Electrophoresis System .....................................................................2
Section 3 Safety Instructions.......................................................................................3
Section 4 Assembly.......................................................................................................4
Section 5 Visualizing Proteins in SDS-Polyacrylamide Gels...................................5
5.1 Visualizing Proteins Stained with Coomassie Blue R-250 ......................................5
5.2 Visualizing Proteins Using Copper Chloride ...........................................................6
5.3 Visualizing Proteins Using Autoradiography ..........................................................6
Section 6 Protein Elution ............................................................................................6
6.1 Elution from SDS-PAGE Gels .................................................................................6
6.2 Elution of Fixed and Stained Proteins ......................................................................7
6.3 Concentration of Eluted Proteins Using Volatile Buffers.........................................7
6.4 Elution of Proteins from Native Gels .......................................................................7
6.5 Removal of SDS from Samples Subsequent to Elution............................................8
Section 7 Visualizing DNA.......................................................................................... 9
7.1 Visualizing DNA in Agarose Gels............................................................................9
7.2 Visualizing DNA in Acrylamide Gels.......................................................................9
Section 8 DNA Elution.................................................................................................9
8.1 Elution from Gel........................................................................................................9
8.2 DNA Purification.....................................................................................................10
Section 9 Recipies.......................................................................................................11
Section 10 Troubleshooting Guide..............................................................................11
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Section 1
Introduction
Elution of proteins and nucleic acids from gel slices is often a difficult and unreliable
procedure. The Model 422 Electro-Eluter solves many of the problems of conventional eluters
by providing easy assembly and eliminating sample loss due to leakage. The Model 422
Electro-Eluter consistently yields between 70% and 100% of the starting material, depending
on the type of elution. The samples are collected in 400-600 µl volumes. The small volume
of eluted material makes subsequent manipulations easy. The Model 422 Electro-Eluter can
run up to six samples at once, with no increase in running time or decrease in yield.
1.1 Specifications
Construction
Electrode module Precision machined acrylic
Buffer chamber and lid Molded polycarbonate
Glass tubes Borosilicate glass
Frits Porous polypropylene
Silicone adaptors Molded silicone
Grommets and stoppers Molded silicone
Membrane caps Molded polyethylene with dialysis membrane
Overall size 16 cm (L) x 12 cm (W) x 18 cm (H)
Elution capacity 1 to 6 samples
Glass tube dimensions 1 cm ID x 6 cm long
Collection volume 400-600 µl
Buffer volume 700 ml
Shipping weight 1.1 kg
Chemical compatibility The Model 422 Electro-Eluter is not
compatible with chlorinated hydrocarbons
(e.g. chloroform), aromatic hydrocarbons
(e.g. toluene, benzene), or acetone. Use of
organic solvents voids all warranties.
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Section 2
Equipment and Reagents
2.1 Equipment
Catalog
Number Product Description
165-2976 Model 422 Electro-Eluter, includes electro-eluter module, 12 Membrane
Caps with a molecular weight cut off (MWCO) of 12,000-15,000 daltons,
6 Glass Tubes with Frits, 6 Silicone Adaptors, 6 Grommets and Stoppers,
buffer tank with lid and cables, and instruction manual
165-2977 Model 422 Electro-Eluter Module, same as 165-2976 without buffer
tank and lid
Accessories
165-2985 Clear Membrane Caps (12), MWCO of 12,000-15,000 daltons
165-2986 Green Membrane Caps (12), MWCO of 3,500 daltons
165-2978 Glass Tubes (6)
165-2981 Silicone Adaptors (6)
165-1988 Grommets and Stoppers (8)
165-2987 Frits (12)
Related Accessories
161-0719 Tris, 1 kg
161-0718 Glycine, 1 kg
161-0400 Coomassie Blue R-250, 10 g
165-4710 Model 1000/500 Power Supply, 120 V, 50/60 Hz
165-4711 Model 1000/500 Power Supply, 220 V, 50/60 Hz
2.2 Modular Mini Electrophoresis System
165-2940 Mini-PROTEAN®II Cell, includes inner cooling core with gaskets,
lower buffer chamber, lid with power cables, Glass Plates (3 sets), clamp
assemblies (2), 10-well, 0.75 mm thick combs (2), casting stand with gaskets, leveling bubble, and instruction manual
170-3930 Mini Trans-Blot®Electrophoretic Transfer Cell, includes 2 gel hold-
ers, electrode assembly, fiber pads, Bio-Ice®cooling unit, and instruction
manual
170-3935 Mini Trans-Blot Module, for use with the Mini-PROTEAN II cell,
same as 170-6530 without buffer tank and lid
165-2960 Mini-PROTEAN II 2-D Cell, includes tube adaptor, sample reservoirs
and stoppers, sample reservoir/capillary tube connectors, Capillary Tubes
with Casting Tube, Tube Gel Ejector, Mini-PROTEAN II slab cell with
inner cooling core and gaskets, lower buffer chamber, lid with power
cables, 2-D combs with 1 standard well (2), 1.0 mm thick spacers (4),
Casting Stand with Gaskets, leveling bubble, and 2-D instruction manual.
165-2965 Mini Tube Cell Module, includes tube adaptor, sample reservoirs and
stoppers, sample reservoir/capillary tube connectors, Capillary Tubes
with Casting Tube, Tube Gel Ejector, and instructions.
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