BIO RAD 419 Prep Cell, Mini Prep Cell Assembly Manual
Mini Prep Cell
Assembly Guide
Catalog #170-2927
Mini Prep Cell Assembly Guide
Cylindrical
holding station
Casting stand
Gel tube assembly
Fig. 1
Cast the Gel
1. Slide the gel tube assembly through the elution chamber top and
secure it on the leveled casting stand with the three screws;
hand tightening is sufficient.
2. Prepare the resolving gel solution as described in the instruction
manual.
3. Use a small syringe with a PTFE (polytetrafluoroethylene) tube
affixed to it to slowly fill the gel tube with monomer mixture
(Figure 1). Gently tap the casting stand against the bench top to
dislodge trapped air bubbles. Visually inspect the bottom of the
gel for bubbles immediately after pouring the gel solution into the
tube.
4. Overlay the gel solution with water-saturated 2-butanol or
tert-amyl alcohol using the narrow PTFE tube on a syringe. Allow
the resolving gel to stand overnight for complete polymerization.
After 1–2 hours of polymerization, replace the alcohol overlay
with gel buffer.
5. Decant or aspirate the buffer overlay and, if appropriate, cast the
stacking gel on top of the resolving gel. Overlay the stacking gel
monomer with water-saturated 2-butanol or tert-amyl alcohol.
Allow the stacking gel to polymerize for 1–2 hours.
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Mini Prep Cell
PTFE tube
Elution manifold base
Fig. 2
Assemble and Purge the Elution Chamber
Assembly Guide
1. Soak the support frit, elution frit, and dialysis membrane in
eletrophoresis buffer.
2. Place the molded harvest ring into the lower portion of the
elution chamber then press in the gray sealing gasket around the
harvest ring to hold the assembly in place.
3. Fill a syringe with electrophoresis buffer and connect it to the
Luer fitting of the 1 mm PTFE tube exiting the base of the
elution manifold (Figure 2). Gently push elution buffer through the
elution tubing (do not pull) until buffer fills the space within the
gray sealing gasket.
4. Place the dialysis membrane on top of the support frit and then
place the elution frit on top of the dialysis membrane.
5. Decant the stacking gel overlay, rinse the surface of the stacking
gel with water, and loosen the three screws holding the column
to the casting stand.
6. Remove the gel tube from the casting stand and place the
assembly on the lower portion of the elution manifold. Hand
tighten the three screws in the threaded holes in the lower
portion of the manifold (Figure 3).
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