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Trans-Blot®Semi-Dry
Quick Reference Guide
Catalog Number 170-3940
Full instructions are provided in the manual and should be read before
using the Trans-Blot SD cell.
I. Steps
1. Prepare transfer buffer and equilibrate acrylamide gel in buffer 20–60
minutes.
2. Cut membrane and filter paper to gel size and wet in buffer 5–10
minutes.
3. Remove safety cover and prepare gel sandwich as follows:
To bottom platinum anode place:
Pre-wet filter paper
Pre-wet membrane
Equilibrated gel
Pre-wet filter paper
4. Secure top stainless steel cathode and safety cover.
5. Run blot.
Optimized conditions will be needed for different proteins.
Guide range:*
Mini-gel 10 V for 30 minutes or 15 V for 15 minutes.
Large gel 25 V for 30 minutes or 15 V for 60 minutes.
Your optimal conditions: Time: Volts:
*Do not exceed 25 volts and 3 mA/cm2current for large gels or 5.5 mA/cm2for mini gels.
6. Turn off the power supply, unplug electrodes, and remove blot.
Note: Roll out air bubbles
between layers.
Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547
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Exploded View of the Trans-Blot SD with Gel Sandwich
Safety Lid
Cathode Plate Assembly
with Latches
Extra Thick Blot Paper
Acrylamide Gel
Membrane
Extra Thick Blot Paper
Anode Plate Assembly
Base
4006066 Rev B
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