Agilent Technologies 7100 User Manual
User Manual
Agilent 7100 Capillary Electrophoresis System
Agilent Technologies
Notices
CAUTION
WARNING
© Agilent Technologies, Inc. 2009
No p art o f this manu al may be re produce d in
any form or by any means (including electronic storage and retrieval or translation
into a foreign language) without prior agreement and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G7100-90000
Edition
12/2009
Printed in Germany
Agilent Technologies, Deutschland GmbH
Hewlett-Packard-Strasse 8
76337 Waldbronn
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The material contained in this document is provided “as is,” and is subject to being changed, without notice,
in future editions. Further, to the maximum extent permitted by applicable
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either express or implied, with regard
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liable for errors or for incidental or
consequential damages in connection with the furnishing, use, or performance of this document or of any
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Safety Notices
A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like
that, if not correctly performed or
adhered to, could result in damage
to the product or loss of important
data. Do not proceed beyond a
CAUTION notice until the indicated
conditions are fully understood and
met.
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly performed or adhered to, could result
in personal injury or death. Do not
proceed beyond a WARNING
notice until the indicated conditions are fully understood and
met.
Research Use Only
Agilent 7100 Capillary Electrophoresis System
In This Guide…
This guide contains information to install and operate the
Agilent 7100 Capillary Electrophoresis System.
1 Introduction
This chapter gives an introduction to the instrument,
instrument overview and internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental
requirements, physical and performance specifications.
3 Installing the Instrument
In this chapter you will find information about how to unpack
and to set up the Agilent CE and information about the
configuration of Instrument.
4 LAN Configuration
This chapter provides information on connecting the CE
instrument to the Agilent ChemStation PC.
5 Using the Agilent 7100 Capillary Electrophoresis System
This chapter provides information on how to set up the CE
instrument for an analysis and explains the basic settings.
6 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and
diagnostic features and the different user interfaces.
7 Hardware Information
This chapter describes the instrument in more detail on
hardware and electronics.
8 Maintenance
This chapter describes the maintenance of the CE instrument.
Agilent 7100 Capillary Electrophoresis System User Manual 3
9 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
10 Appendix
This chapter provides additional information on safety, legal
and web content associated with your 7100 CE instrument.
4 Agilent 7100 Capillary Electrophoresis System User Manual
Contents
1 Introduction 13
Introduction to the Agilent 7100 Capillary Electrophoresis System 14
Getting Help 14
Where to Find Information 15
What Learning Products are Available? 16
2 Site Requirements and Specifications 17
Site Requirements 18
Power Consideration 18
Power Cords 19
Bench Space 20
Environment 20
Physical Specifications 21
Performance Specifications 22
Anforderungen an den Aufstellungsort 23
Stromversorgung 23
Netzkabel 24
Platzbedarf 25
Umgebungsbedingungen 25
Abmessungen und Gewicht 26
Leistungsspezifikationen 27
Exigences d’installation 28
Remarques à propos de l’alimentation 28
Câbles d’alimentation 29
Encombrement 30
Environnement 30
Caractéristiques physiques 31
Caractéristiques de performance 32
Agilent 7100 Capillary Electrophoresis System User Manual 5
Contents
Requisiti ambientali 33
Alimentazione 33
Cavi di alimentazione 34
Spazio necessario 35
Ambiente 35
Specifiche fisiche 36
Specifiche delle prestazioni 37
Requisitos de las instalaciones 38
Consideraciones sobre la corriente 38
Cables de alimentación 39
Espacio en el banco 40
Entorno 40
Especificaciones físicas 41
Especificaciones de rendimiento 42
設置について 43
電源について 43
電源コード 44
作業台スペース 45
環境条件 45
物理的仕様 46
性能仕様 47
场地要求 48
电源要求 48
电源线 49
工作台位置 50
环境 50
物理规格 51
性能规格 52
3 Installing the Instrument 53
Unpacking the Agilent CE Instrument 54
Damaged Packaging 54
Unpacking the CE Instrument 54
6 Agilent 7100 Capillary Electrophoresis System User Manual
Delivery Checklist 55
Installing the Instrument 57
Physical Connection 57
4 LAN Configuration 61
What You Have to Do First 62
TCP/IP Parameter Configuration 63
Configuration Switch 64
Initialization Mode Selection 65
Link Configuration Selection 69
Storing the Settings Permanently with Bootp 70
Manual Configuration 71
With Telnet 72
Agilent Bootp Service 75
Location of the Media Access Control (MAC) Address 76
5 Using the Agilent 7100 Capillary Electrophoresis System 77
System Definition 78
Controlling the Agilent CE Instrument 79
Before You Start the Agilent CE Instrument 79
Starting Up the Agilent CE Instrument 79
Starting Your Computer and the Agilent ChemStation Software 81
Starting the Agilent ChemStation Software the First Time 82
Working with the CE Diagram 84
Common Software Toolbar 84
Software Toolbar 85
Graphical User Interface Explanation 86
Menus in the CE and DAD Diagram 88
System Vialtable 90
How to Prepare your Agilent CE Instrument for Analysis 91
Capillary Electrophoresis, CE 92
How to Use Your Agilent CE Instrument for Capillary Electrophoresis
Separations 92
Contents
Agilent 7100 Capillary Electrophoresis System User Manual 7
Contents
Agilent CE Method Parameters 92
Vials 96
Cassette Temperature 97
High Voltage System 98
Posttime and Stoptime 100
Replenishment 100
Preconditioning 101
Injection 103
Timetable 105
Detector Parameters 107
Switching the Lamp On 110
Preparing the Replenishment System if Needed 111
Preparing Vials for Buffer and Sample 115
Installing a Capillary in the Alignment Interface 124
Installing a Capillary in an Empty Capillary Cassette 128
Inserting the Cassette 132
Fraction Collection 135
Capillary Electrophoresis, Plus High Pressure CE+p 136
Requirements 136
Outlet Lift Connection to the Tubing System 138
Preparing the Agilent CE Instrument for High Pressure Use 140
Operation and Function in CE+p Mode 143
Injection Options with CE+p 146
Timetable Options with CE+p 146
CE+p Control Using the CE Diagram 147
Capillary Electrochromatography, CEC 148
How to Use Your Agilent CE Instrument for Capillary
Electrochromatography 148
Preparing the Agilent CE Instrument for High Pressure Use 150
CEC-Specific GUI Explanation 153
Injection Options with CEC 156
Timetable Options with CEC 157
CEC Control Using the CE Diagram 158
8 Agilent 7100 Capillary Electrophoresis System User Manual
Running the CEC Analysis 159
6 Troubleshooting and Diagnostics 161
CE Problem Solving 162
Power On Fail 162
Leaks 163
Blocked Replenishment Needle 165
Leakage Current 165
Broken Capillary 166
Contaminated Insulation Plate 166
Empty Injection Vial 167
Dirt in the Vials 167
Air Bubble in Injection Vial or Run Buffer Vials 167
Air Bubble in Capillary 167
Problems with the Replenishment System and Buffers
Containing SDS 168
Agilent Lab Advisor Software 169
Configuration 169
Contents
7 Hardware Information 171
Identifying Instrument Components 172
7100 Capillary Electrophoresis Instrument Electronics 173
Firmware 176
Resident System 176
Main System 176
Firmware Updates 177
Diode Array Detector 179
DAD Control 180
Digital to Analog Converter 181
Changing the Fuses for the Power Supplies 182
Installing Drainage Tubing 183
External Water Bath for Tray Cooling (Optional) 184
Analog to Digital Converter 186
Agilent 7100 Capillary Electrophoresis System User Manual 9
Contents
8 Maintenance 189
Standard Operating Procedures for Maintenance of Your
Agilent CE Instrument 190
Overview of Maintenance 191
Early Maintenance Feedback (EMF) 194
EMF Counters 194
Using the EMF Counters 194
Setting the EMF Limits 195
Cleaning the Electrodes, Pre-punchers and Insulation Plate 196
Prepare the Agilent CE Instrument 197
Remove the Covers 198
Accessing the Electrodes 199
Accessing the Liquid Handling Module 201
Accessing and Removing the Pre-punchers 203
Cleaning the Electrodes 205
Cleaning the Pre-punchers 206
Cleaning the Insulation Plate 207
Reinstalling the Pre-punchers 208
Reinstalling the Electrodes and the Insulation Plate 210
Reinstall the Capillary Cassette 215
Reinstall the Front Cover 215
Maintenance of the Replenishment System 216
Changing the Buffer Composition in the Replenishment System 217
The Replenishment System Will Not Be Used for Some Time 218
Further Action 219
Operations for Replenishment Maintenance 219
Changing the Air Inlet Filter 222
Accessing the Air Filter 222
Changing the Lamp 224
Removing the Existing Lamp 225
Inserting a New Lamp 227
Further Action 227
Cleaning the Instrument 228
10 Agilent 7100 Capillary Electrophoresis System User Manual
9 Parts and Materials for Maintenance 229
Overview of Maintenance Parts 230
10 Appendix 231
General Safety Information 232
Operation 232
Allgemeine Sicherheitsinformation 235
Informations générales de sécurité 238
Informazioni generali sulla sicurezza 241
Información de seguridad 244
安全に関する一般的な情報 247
一般安全信息 250
Setting Up a Test Sample Run 252
Method Parameter CE (Default Settings) 253
Method Parameter DAD 255
Chemical and Biological Safety 256
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 257
Radio Interference 258
Sound Emission 259
Legal Notice 260
Agilent Technologies on Internet 261
Contents
Index 263
Agilent 7100 Capillary Electrophoresis System User Manual 11
Contents
12 Agilent 7100 Capillary Electrophoresis System User Manual
Agilent 7100 Capillary Electrophoresis System
User Manual
1
Introduction
Introduction to the Agilent 7100 Capillary Electrophoresis System 14
Where to Find Information 15
What Learning Products are Available? 16
This chapter gives an introduction to the Agilent 7100 Capillary
Electrophoresis System and where to find specific information or help.
Agilent Technologies
13
1 Introduction
Introduction to the Agilent 7100 Capillary Electrophoresis System
Introduction to the Agilent 7100 Capillary Electrophoresis
System
This handbook gives an overview of your Agilent 7100 Capillary
Electrophoresis System. It is designed to get you started working with the
system. We strongly advise you to make extensive use of the online help when
working with the system. There you find detailed reference and task
information which complements the overview given in this guide. Refer to
“Getting Help” later in this section for information on using the online help of
the ChemStation. Refer to “Where to Find Information” for help in finding
information on certain tasks.
Getting Help
You can get help by choosing the Help item from the top menu. This puts you
into the help index. From there you can browse through the information by
selecting the appropriate jumps (underlined words). To access specific
information in the help:
When you are in the Agilent ChemStation or Lab Advisor software:
• select a context-sensitive element and press F1.
When you are in the online help:
• search for a specific keyword using the search command,
• select a jump - a jump is an underlined word you can select to go to a topic
related to the word, or
• select a word or phrase with a dotted underline to see its definition. The
online help also contains online information on how to use help.
14 Agilent 7100 Capillary Electrophoresis System User Manual
Where to Find Information
Tabl e 1 Where to find information
Task Refer to
Introduction 1
Where to Find Information
• Installation of the Agilent CE System,
installing the external water bath,
installing the high sensitivity cell
• Setting up the Agilent CE System for
CE analysis, CE+p
• How to use the Agilent CE System in
the CE+p mode
• How to use the Agilent CE System for
capillary electrochromatography
• How to maintain the Agilent CE
System
• Setting up a sequence Online help "Understanding Your ChemStation"
• Data analysis Online help "Understanding Your ChemStation"
• Setting up a report Online help "Understanding Your ChemStation"
• Spectral library search “ChemStation Applications” handbook and online help
• Setting up customized reports “ChemStation Applications” handbook
• Safety information Safety information in “Appendix” on page 231
• Learning ChemStation concepts “Understanding Your ChemStation” handbook
• Learning the theory of the technique High Performance Capillary Electrophoresis: An
"The Core Agilent CE Instrument" “Installing the
Instrument” on page 53
Chapter “Using the Agilent 7100 Capillary
Electrophoresis System” on page 77"
“Capillary Electrophoresis, Plus High Pressure
CE+p” on page 136
“Capillary Electrochromatography, CEC” on page 148
Chapter “Maintenance” on page 189
Introduction”
The CE Partner CD-ROM: “The comprehensive,
interactive tool for beginners and advanced users of
CE”
• Application Agilent web page (http://www.agilent.com), search for
Application notes
Agilent 7100 Capillary Electrophoresis System User Manual 15
1 Introduction
What Learning Products are Available?
What Learning Products are Available?
The Agilent CE System is supplied with the following:
• User's Guide
• High Performance Capillary Electrophoresis: An Introduction
The Agilent ChemStation and Lab Advisor/Instrument Utilities software DVD
is supplied with the following electronic documents:
• Installing Your Agilent ChemStation for LC and CE Systems
• Installing and Understanding Your Spectra Module
• Understanding Your Agilent ChemStation
• Online help
• Understanding Your Agilent ChemStation for CE Systems
16 Agilent 7100 Capillary Electrophoresis System User Manual
Agilent 7100 Capillary Electrophoresis System
User Manual
2
Site Requirements and Specifications
Site Requirements 18
Physical Specifications 21
Performance Specifications 22
Anforderungen an den Aufstellungsort 23
Abmessungen und Gewicht 26
Leistungsspezifikationen 27
Exigences d’installation 28
Caractéristiques physiques 31
Caractéristiques de performance 32
Requisiti ambientali 33
Specifiche fisiche 36
Specifiche delle prestazioni 37
Requisitos de las instalaciones 38
Especificaciones físicas 41
Especificaciones de rendimiento 42
設置について 43
物理的仕様 46
性能仕様 47
场地要求 48
物理规格 51
性能规格 52
This chapter provides information on the environmental requirements, as well
as physical and performance specifications.
Agilent Technologies
17
2 Site Requirements and Specifications
WARNING
WARNING
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
7100 Capillary Electrophoresis instrument.
Before you begin installation, check carefully that the place you have chosen
meets the requirements below.
Power Consideration
The instrument power supply has wide ranging capabilities and accepts any
standard line voltage in the range mentioned in Table 2 on page 21.
Consequently, there is no voltage selector on the back of the detector.
The module is partially energized when switched off, as long as the power cord is
plugged in.
18 Agilent 7100 Capillary Electrophoresis System User Manual
Repair or maintenance work on the module can lead to personal injuries, for
example a shock hazard, when the cover is opened and the module is connected to
the power.
• Make sure that it is always possible to access the power plug.
• Remove the power cable from the instrument before opening the cover.
• Do not connect the power cable to the instrument while the electronic box in the
back of the instrument is opened or side panels are removed.
Incorrect line voltage on the instrument
Shock hazard or damage to your instrument can result if the device is connected to a
line voltage that is higher than specified.
• Connect your instrument to the specified line voltage.
CAUTION
Inaccessible power plug.
WARNING
WARNING
In the case of an emergency it must be possible to disconnect the instrument from the
power line at any time.
• Make sure the power connector of the instrument can be easily reached and
unplugged.
• Provide sufficient space behind the power socket of the instrument to unplug the
cable.
Power Cords
Different power cords are delivered with the instrument. The female end of all
power cords is identical. It plugs into the power-input socket at the rear. The
male end of each power cord is different and designed to match the wall socket
of a particular country or region.
Absence of a ground connection or use of an unspecified power cord
Site Requirements and Specifications 2
Site Requirements
The absence of a ground connection or the use of an unspecified power cord can
lead to an electric shock or short circuit.
• Never operate your instrument from a power outlet without a ground connection.
• Never use a power cord other than the Agilent Technologies power cord designed
for your region.
Use of unsupplied cables
Using cables not supplied by Agilent Technologies can lead to damage of the
electronic components or personal injury.
• Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.
Agilent 7100 Capillary Electrophoresis System User Manual 19
2 Site Requirements and Specifications
CAUTION
Site Requirements
Bench Space
The instrument dimensions and weight mean that the instrument can be
placed on almost any desk or laboratory bench. It needs an additional 2.5 cm
(1.0 inch) of space on either side and approximately 8 cm (3.1 inches) in the
rear for air circulation and electric connections.
If the Agilent 7100 Capillary Electrophoresis instrument is to be placed on a
bench, make sure that the bench is designed to bear the weight of the system.
The instrument must be operated in a upright position.
Environment
Your instrument will work within the specifications for ambient temperatures
and relative humidity described in Table 2 on page 21.
Condensation within the module
Condensation will damage the system electronics.
20 Agilent 7100 Capillary Electrophoresis System User Manual
• Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
• If your module was shipped in cold weather, leave it in its box and allow it to warm
slowly to room temperature to avoid condensation.
Physical Specifications
NOTE
Tabl e 2 Physical specifications
Type Specification Comments
Weight 35 kg (77.2 lbs)
Site Requirements and Specifications 2
Physical Specifications
Dimensions
(width × depth × height)
Line voltage 100–240 VAC ± 10% Wide-ranging capability
Line frequency 50 or 60 Hz ± 5%
Power consumption 350 VA / 300 W / 1024 BTU/h Maximum
Ambient operating
temperature
Ambient non-operating
temperature
Humidity below 80% at 31 °C (87.8 °F) Non-condensing
Operating altitude Up to 2000 m (6500 ft)
External cooling max. 0.5 bar (7.2 psi),
External pressure 2-12 bar (29-174 psi) Oil-free air or nitrogen
Safety standards: IEC,
CSA, UL
Housing
350 x 510 x 590 mm
(13.8 x 20.1 x 23.2 inches)
5–40 °C (41–104 °F)
-40 to 70°C (-40 to 158 °F)
max. 50 °C (122 °F)
Installation category II,
Pollution degree 2
All materials are recyclable
Waterbath
For indoor use only
For applications run in CEC mode (Capillary Electrochromatography) or CE+p
mode (Capillary Electrophoresis mode with optional usage of higher pressure), an
external pressure supply of oil-free air or nitrogen with a maximum pressure of 15
bar (218 psi) can be used. A respective male adapter that fits into the instrument’s
female adapter and PTFE tubing are part of the shipment.
The Agilent 7100 Capillary Electrophoresis System is designed to operate in a typical
electromagnetic environment where RF transmitters, such as mobile phones, should not be
used in close proximity.
Agilent 7100 Capillary Electrophoresis System User Manual 21
2 Site Requirements and Specifications
Performance Specifications
Performance Specifications
Tabl e 3 Performance specifications for the Agilent 7100 Capillary Electrophoresis System
Type Specification Comments
Safety features Extensive diagnostics, error detection and display,
current leak detection; low current limit
Liquid leak sensor; safety sensors on door and
cover disabling high voltage; vial sensor;
Control and data evaluation Agilent ChemStation under Windows® XP (SP3)
Communication LAN, controller-area network (CAN), RS-232C,
APG remote: ready, start, stop and shut-down
signals. USB (future use).
Analog out and in.
GLP features Early maintenance feedback (EMF), electronic
records of maintenance and errors
0 to1 V
22 Agilent 7100 Capillary Electrophoresis System User Manual
Anforderungen an den Aufstellungsort
WARNUNG
WARNUNG
Eine geeignete Umgebung ist wichtig für die optimale Leistungsfähigkeit
des 7100 Kapillarelektrophorese-Geräts.
Prüfen Sie sorgfältig, ob der von Ihnen gewählte Aufstellort die nachfolgenden
Anforderungen erfüllt, bevor Sie mit der Installation beginnen.
Stromversorgung
Das Weitbereichsnetzteil des Geräts arbeitet mit Standard-Netzspannungen
gemäß den Angaben in Tabelle 4 auf Seite 26. Daher ist an der Rückseite
des Detektors kein Spannungswahlschalter vorhanden.
Auch im ausgeschalteten Zustand fließt im Modul Strom, solange das Netzkabel
eingesteckt ist.
Site Requirements and Specifications 2
Anforderungen an den Aufstellungsort
Agilent 7100 Capillary Electrophoresis System User Manual 23
Die Durchführung von Reparatur- oder Wartungsarbeiten am Modul kann zu
Personenschäden wie z. B. durch Stromschlag führen, wenn das Modulgehäuse
geöffnet wird, während das Modul an die Netzspannung angeschlossen ist.
• Stellen Sie den freien Zugang zu den Netzkabeln sicher.
• Trennen Sie das Netzkabel vom Gerät, bevor Sie das Gehäuse öffnen.
• Schließen Sie das Netzkabel nicht an das Gerät an, während der Elektronikkasten
an der Geräterückseite geöffnet oder Seitenteile entfernt sind.
Falsche Netzspannung am Gerät
Wenn das Gerät an eine höhere Spannung als spezifiziert angeschlossen wird, kann
dies zu Überspannungsschäden oder einer völligen Zerstörung des Geräts führen.
• Schließen Sie das Gerät an die angegebene Netzspannung an.
2 Site Requirements and Specifications
VORSICHT
WARNUNG
WARNUNG
Anforderungen an den Aufstellungsort
Unzugänglicher Netzstecker.
In einem Notfall muss es jederzeit möglich sein, das Gerät vom Stromnetz zu trennen.
• Stellen Sie sicher, dass der Netzstecker des Geräts leicht zugänglich ist.
• Lassen Sie hinter der Netzbuchse des Geräts genügend Platz zum Herausziehen
des Kabels.
Netzkabel
Das Gerät wird mit unterschiedlichen Netzkabeln ausgeliefert. Der weibliche
Stecker ist bei jedem Netzkabel identisch. Er wird an die Netzanschlussbuchse
an der Rückseite angeschlossen. Die Stecker am anderen Ende der Netzkabel
sind unterschiedlich und erfüllen die Normen unterschiedlicher Länder oder
Regionen.
Nicht vorhandene Erdung oder Verwendung eines nicht spezifizierten Netzkabels
Bei der Verwendung des Geräts ohne Erdung oder mit einem nicht spezifizierten
Netzkabel können Stromschläge und Kurzschlüsse auftreten.
24 Agilent 7100 Capillary Electrophoresis System User Manual
• Betreiben Sie Ihr Gerät niemals an einer Spannungsquelle ohne Erdung.
• Verwenden Sie niemals ein anderes als das von Agilent zum Einsatz im jeweiligen
Land bereitgestellte Kabel.
Verwendung nicht im Lieferumfang enthaltener Kabel
Die Verwendung von Kabeln, die nicht von Agilent Technologies geliefert
wurden, kann zu einer Beschädigung der elektronischen Komponenten oder
zu Personenschäden führen.
• Verwenden Sie ausschließlich Originalkabel von Agilent Technologies, um eine
einwandfreie Funktion und die Einhaltung der Sicherheits- und EML-Bestimmungen
zu gewährleisten.
Platzbedarf
VORSICHT
Die Abmessungen und das Gewicht des Geräts ermöglichen seine Aufstellung
auf praktisch jedem Schreibtisch oder Laborarbeitstisch. Das Gerät benötigt
an jeder Seite zusätzlich 2,5 cm Platz und ungefähr 8 cm an der Rückseite
für die elektrischen Anschlüsse und für eine ausreichende Luftzirkulation.
Wenn das Agilent 7100 Kapillarelektrophorese System auf einem Labortisch
aufgestellt werden soll, stellen Sie sicher, dass der Tisch ausreichende
Tragfähigkeit für das System aufweist.
Das Gerät ist stehend zu betreiben!
Umgebungsbedingungen
Ihr Gerät arbeitet bei der Umgebungstemperatur und der relativen
Luftfeuchtigkeit, die in den Spezifikationen in Tabelle 4 auf Seite 26
angegeben sind.
Kondensation im Inneren des Moduls
Site Requirements and Specifications 2
Anforderungen an den Aufstellungsort
Agilent 7100 Capillary Electrophoresis System User Manual 25
Kondensation führt zur Beschädigung der Systemelektronik.
• Vermeiden Sie die Lagerung, den Versand oder den Betrieb der Pumpe unter
Bedingungen, die zu einer Kondensation in der Pumpe führen können.
• Nach einem Transport bei niedrigen Temperaturen muss das Gerät zur Vermeidung
von Kondensation in der Verpackung verbleiben, bis es sich auf Raumtemperatur
erwärmt hat.
2 Site Requirements and Specifications
HINWEIS
Abmessungen und Gewicht
Abmessungen und Gewicht
Tabelle 4 Abmessungen und Gewicht
Typ Spezifikationen Kommentare
Gewicht 35 kg
Abmessungen
(Breite × Tiefe × Höhe)
Netzspannung 100–240 V Wechselstrom ± 10 % Weitbereichsnetzteil
Netzfrequenz 50 oder 60 Hz ± 5 %
Leistungsaufnahme 350 VA / 300 W / 1024 BTU/h Maximal
zulässige
Umgebungstemperatur
bei Lagerung
Umgebungstemperatur
bei Lagerung
Luftfeuchtigkeit unter 80 % bei 31 °C Nicht kondensierend
Betriebshöhe bis zu 2000 m
350 x 510 x 590 mm
(23,2 x 13,8 x 20,1 Zoll)
5–40 °C
-40 bis 70 °C
26 Agilent 7100 Capillary Electrophoresis System User Manual
Externe Kühlung max. 0,5 bar (7,2 psi), max. 50 °C Wasserbad
Externer Druck 2-12 bar (29-174 psi) Ölfreie Luft oder Stickstoff
Sicherheitsstandards: IEC,
CSA, UL
Gehäuse
Installationskategorie II,
Verschmutzungsgrad 2.
Alle Materialien sind recyclebar.
Nur für den Einsatz im
Innenbereich geeignet
Für Applikationen im CEC-Modus (Capillary Electrochromatography) oder
CE+p-Modus (Kapillarelektrophorese-Modus mit optionaler Verwendung höheren
Druckes) kann eine externe Druckversorgung mit ölfreier Luft oder Stickstoff und
einem Druckmaximum von 15 bar (218 psi) verwendet werden. Ein
entsprechender männlicher Adapter für den weiblichen Adapter des Geräts und
PTFE-Schläuche werden mitgeliefert.
Das Agilent 7100 Kapillarelektrophorese-System ist auf den Betrieb in einer typischen
elektromagnetischen Umgebung ausgelegt, in deren unmittelbarer Nähe keine HF-Sender
wie z. B. Mobiltelefone verwendet werden dürfen.
Site Requirements and Specifications 2
Leistungsspezifikationen
Leistungsspezifikationen
Tabelle 5 Leistungsspezifikationen für das Agilent 7100 Kapillarelektrophorese-System
Typ Spezifikationen Kommentare
Sicherheitsvorkehrungen Umfangreiche Diagnose, Fehlererkennung und
-anzeige, Kriechstromerkennung; Grenzwert für
zu niedrigen Strom
Flüssigkeitslecksensor; Sicherheitssensoren
an Tür und Abdeckung zur Trennung der
Hochspannung; Probenflaschensensor;
Steuerung und
Datenauswertung
Kommunikation LAN, CAN (Controller-Area Network), RS-232C,
GLP-Eigenschaften Frühwarnsystem für fällige Wartungen (EMF, Early
Agilent ChemStation unter Windows® XP (SP3)
APG Remote: Ready-, Start-, Stop- und
Shut-down-Signale. USB (zukünftige
Ver wen dung) .
Analoger Ein-/Ausgang.
Maintenance Feedback), elektronische
Aufzeichnung von Wartungsarbeiten und
Fehlermeldungen
0 bis 1 V
Agilent 7100 Capillary Electrophoresis System User Manual 27
2 Site Requirements and Specifications
AVERTISSEMENT
AVERTISSEMENT
Exigences d’installation
Exigences d’installation
Un environnement adéquat est indispensable pour obtenir des performances
optimales de l’instrument d’électrophorèse capillaire 7100.
Avant de commencer l’installation, vérifiez soigneusement que l’emplacement
choisi est conforme aux exigences ci-dessous.
Remarques à propos de l’alimentation
Le module d’alimentation de l’instrument présente une tolérance importante.
Il accepte ainsi n’importe quelle tension de secteur se situant dans la plage
de tolérance précisée dans le Tableau 6, page 31. Ceci explique l’absence
de sélecteur de tension à l’arrière du détecteur.
Le module reste partiellement activé lorsqu’il est éteint, tant que le cordon
d’alimentation est branché.
Certaines opérations de réparation ou d’entretien sur le module peuvent
occasionner des blessures, par exemple l’électrocution, si le capot est
ouvert et que le module est branché.
•
Veillez à ce que la prise d’alimentation électrique reste à tout moment accessible.
• Débranchez le câble d’alimentation de l’instrument avant d’ouvrir le capot.
• Ne branchez pas le câble d’alimentation sur l’instrument si le boîtier
électronique à l’arrière est ouvert ou si les panneaux latéraux sont enlevés.
Tension de secteur incorrecte au niveau de l’instrument
Il existe un risque d’électrocution ou de dommages à l’instrument en cas de
raccordement à une ligne d’alimentation de tension supérieure à celle
spécifiée.
• Connectez l’instrument à la tension indiquée.
28 Agilent 7100 Capillary Electrophoresis System User Manual
ATTENTION
Prise d’alimentation inaccessible.
AVERTISSEMENT
AVERTISSEMENT
En cas d’urgence, il doit être possible de débrancher à tout moment l’instrument
du secteur.
• Veillez à faciliter l’accès au connecteur d’alimentation de l’instrument
et à permettre de le débrancher facilement.
• Maintenez un espace suffisant derrière la prise d’alimentation de l’instrument
pour pouvoir débrancher le câble.
Câbles d’alimentation
Différents câbles d’alimentation sont livrés avec l’instrument. L’extrémité
femelle est la même pour tous les câbles. Elle s’enfiche dans l’embase
d’alimentation située à l’arrière. L’extrémité mâle, qui se branche sur la prise
de courant murale, varie selon le pays ou la région.
Absence de raccordement à la terre et utilisation d’un câble d’alimentation
non adapté
Site Requirements and Specifications 2
Exigences d’installation
Agilent 7100 Capillary Electrophoresis System User Manual 29
L’absence de raccordement à la terre et l’utilisation d’un câble d’alimentation
non adapté peuvent entraîner une électrocution ou un court-circuit.
• N’utilisez jamais une prise de courant sans mise à la terre.
• N’utilisez jamais de câble d’alimentation autre que le modèle Agilent
Technologies destiné à votre pays.
Utilisation de câbles non fournis
L’utilisation de câbles non fournis par Agilent Technologies pourrait
endommager les composants électroniques ou provoquer des blessures.
• Pour garantir un bon fonctionnement et le respect des règles de sécurité ou de
compatibilité électromagnétique, n’utilisez jamais d’autres câbles que ceux
fournis par Agilent Technologies.
2 Site Requirements and Specifications
ATTENTION
Exigences d’installation
Encombrement
Les dimensions et le poids de l’instrument permettent un placement sur
quasiment n’importe quel bureau ou paillasse de laboratoire. Veillez à prévoir
un dégagement de 2,5 cm de part et d’autre et d’environ 8 cm à l’arrière pour
assurer la circulation de l’air et le passage des raccordements électriques
Si le système d’électrophorèse capillaire Agilent 7100 doit être placé sur une
paillasse, assurez-vous que celle-ci est conçue pour supporter le poids du
système.
En fonctionnement, l’instrument doit être en position verticale.
Environnement
Votre instrument fonctionnera conformément aux spécifications dans les
conditions de température ambiante et d’humidité relative précisées dans le
Tableau 6, page 31.
Condensation à l’intérieur du module
30 Agilent 7100 Capillary Electrophoresis System User Manual
La condensation endommage les circuits électroniques du système.
• N’entreposez pas, ne transportez pas et n’utilisez pas votre module dans des
conditions où les fluctuations de température risqueraient de provoquer de
la condensation à l’intérieur du module.
• Si le module a été transporté par temps froid, ne le sortez directement pas de
son emballage : laissez-le atteindre progressivement la température ambiante
pour éviter toute condensation.
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