Agilent Technologies 7100 User Manual

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User Manual

Agilent 7100 Capillary Electrophoresis System

Agilent Technologies

Notices

CAUTION

WARNING

any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.

Manual Part Number

G7100-90000

Edition

12/2009 Printed in Germany Agilent Technologies, Deutschland GmbH

Hewlett-Packard-Strasse 8 76337 Waldbronn

Warranty

The material contained in this document is provided “as is,” and is subject to being changed, without notice, in future editions. Further, to the maximum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a particular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or performance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the separate agreement shall control.

Technology Licenses

The hardware and/or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license.

Restricted Rights Legend

If software is for use in the performance of a U.S. Government prime contract or subcontract, Software is delivered and licensed as “Commercial computer software” as defined in DFAR 252.227-7014 (June 1995), or as a “commercial item” as defined in FAR

2.101(a) or as “Restricted computer software” as defined in FAR 52.227-19 (June

1987) or any equivalent agency regulation or contract clause. Use, duplication or disclosure of Software is subject to Agilent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will receive no greater than Restricted Rights as

defined in FAR 52.227-19(c)(1-2) (June

1987). U.S. Government users will receive no greater than Limited Rights as defined in FAR 52.227-14 (June 1987) or DFAR

252.227-7015 (b)(2) (November 1995), as applicable in any technical data.

Safety Notices

A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.

A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met.

Research Use Only

Agilent 7100 Capillary Electrophoresis System

In This Guide…

This guide contains information to install and operate the Agilent 7100 Capillary Electrophoresis System.

1 Introduction

This chapter gives an introduction to the instrument, instrument overview and internal connectors.

2 Site Requirements and Specifications

This chapter provides information on environmental requirements, physical and performance specifications.

3 Installing the Instrument

In this chapter you will find information about how to unpack and to set up the Agilent CE and information about the configuration of Instrument.

4 LAN Configuration

This chapter provides information on connecting the CE instrument to the Agilent ChemStation PC.

5 Using the Agilent 7100 Capillary Electrophoresis System

This chapter provides information on how to set up the CE instrument for an analysis and explains the basic settings.

6 Troubleshooting and Diagnostics

This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces.

7 Hardware Information

This chapter describes the instrument in more detail on hardware and electronics.

8 Maintenance

This chapter describes the maintenance of the CE instrument.

Agilent 7100 Capillary Electrophoresis System User Manual 3

9 Parts and Materials for Maintenance

This chapter provides information on parts for maintenance.

10 Appendix

This chapter provides additional information on safety, legal and web content associated with your 7100 CE instrument.

4 Agilent 7100 Capillary Electrophoresis System User Manual

1 Introduction 13

Introduction to the Agilent 7100 Capillary Electrophoresis System 14

Getting Help 14 Where to Find Information 15 What Learning Products are Available? 16

2 Site Requirements and Specifications 17

Site Requirements 18

Power Consideration 18

Power Cords 19

Bench Space 20

Environment 20 Physical Specifications 21 Performance Specifications 22 Anforderungen an den Aufstellungsort 23

Stromversorgung 23

Netzkabel 24

Platzbedarf 25

Umgebungsbedingungen 25 Abmessungen und Gewicht 26 Leistungsspezifikationen 27 Exigences d’installation 28

Remarques à propos de l’alimentation 28

Câbles d’alimentation 29

Encombrement 30

Environnement 30 Caractéristiques physiques 31 Caractéristiques de performance 32

Agilent 7100 Capillary Electrophoresis System User Manual 5

Contents

Requisiti ambientali 33

Alimentazione 33

Cavi di alimentazione 34

Spazio necessario 35

Ambiente 35 Specifiche fisiche 36 Specifiche delle prestazioni 37 Requisitos de las instalaciones 38

Consideraciones sobre la corriente 38

Cables de alimentación 39

Espacio en el banco 40

Entorno 40 Especificaciones físicas 41 Especificaciones de rendimiento 42

設置について 43

電源について 43

電源コード 44

作業台スペース 45

環境条件 45 物理的仕様 46 性能仕様 47

场地要求 48

电源要求 48

电源线 49

工作台位置 50

环境 50 物理规格 51 性能规格 52

3 Installing the Instrument 53

Unpacking the Agilent CE Instrument 54

Damaged Packaging 54

Unpacking the CE Instrument 54

6 Agilent 7100 Capillary Electrophoresis System User Manual

Delivery Checklist 55 Installing the Instrument 57

Physical Connection 57

4 LAN Configuration 61

What You Have to Do First 62 TCP/IP Parameter Configuration 63 Configuration Switch 64 Initialization Mode Selection 65 Link Configuration Selection 69 Storing the Settings Permanently with Bootp 70 Manual Configuration 71

With Telnet 72 Agilent Bootp Service 75 Location of the Media Access Control (MAC) Address 76

5 Using the Agilent 7100 Capillary Electrophoresis System 77

System Definition 78 Controlling the Agilent CE Instrument 79

Before You Start the Agilent CE Instrument 79

Starting Up the Agilent CE Instrument 79

Starting Your Computer and the Agilent ChemStation Software 81

Starting the Agilent ChemStation Software the First Time 82 Working with the CE Diagram 84

Common Software Toolbar 84

Software Toolbar 85

Graphical User Interface Explanation 86

Menus in the CE and DAD Diagram 88

System Vialtable 90 How to Prepare your Agilent CE Instrument for Analysis 91 Capillary Electrophoresis, CE 92

How to Use Your Agilent CE Instrument for Capillary Electrophoresis

Separations 92

Contents

Agilent 7100 Capillary Electrophoresis System User Manual 7

Contents

Agilent CE Method Parameters 92

Vials 96

Cassette Temperature 97

High Voltage System 98

Posttime and Stoptime 100

Replenishment 100

Preconditioning 101

Injection 103

Timetable 105

Detector Parameters 107

Switching the Lamp On 110

Preparing the Replenishment System if Needed 111

Preparing Vials for Buffer and Sample 115

Installing a Capillary in the Alignment Interface 124

Installing a Capillary in an Empty Capillary Cassette 128

Inserting the Cassette 132

Fraction Collection 135 Capillary Electrophoresis, Plus High Pressure CE+p 136

Requirements 136

Outlet Lift Connection to the Tubing System 138

Preparing the Agilent CE Instrument for High Pressure Use 140

Operation and Function in CE+p Mode 143

Injection Options with CE+p 146

Timetable Options with CE+p 146

CE+p Control Using the CE Diagram 147 Capillary Electrochromatography, CEC 148

How to Use Your Agilent CE Instrument for Capillary

Electrochromatography 148 Preparing the Agilent CE Instrument for High Pressure Use 150 CEC-Specific GUI Explanation 153 Injection Options with CEC 156 Timetable Options with CEC 157 CEC Control Using the CE Diagram 158

8 Agilent 7100 Capillary Electrophoresis System User Manual

Running the CEC Analysis 159

6 Troubleshooting and Diagnostics 161

CE Problem Solving 162

Power On Fail 162 Leaks 163 Blocked Replenishment Needle 165 Leakage Current 165 Broken Capillary 166 Contaminated Insulation Plate 166 Empty Injection Vial 167 Dirt in the Vials 167 Air Bubble in Injection Vial or Run Buffer Vials 167 Air Bubble in Capillary 167 Problems with the Replenishment System and Buffers

Containing SDS 168

Agilent Lab Advisor Software 169

Configuration 169

Contents

7 Hardware Information 171

Identifying Instrument Components 172 7100 Capillary Electrophoresis Instrument Electronics 173 Firmware 176

Resident System 176 Main System 176 Firmware Updates 177

Diode Array Detector 179

DAD Control 180 Digital to Analog Converter 181

Changing the Fuses for the Power Supplies 182 Installing Drainage Tubing 183 External Water Bath for Tray Cooling (Optional) 184 Analog to Digital Converter 186

Agilent 7100 Capillary Electrophoresis System User Manual 9

Contents

8 Maintenance 189

Standard Operating Procedures for Maintenance of Your

Agilent CE Instrument 190

Overview of Maintenance 191 Early Maintenance Feedback (EMF) 194

EMF Counters 194 Using the EMF Counters 194 Setting the EMF Limits 195

Cleaning the Electrodes, Pre-punchers and Insulation Plate 196

Prepare the Agilent CE Instrument 197 Remove the Covers 198 Accessing the Electrodes 199 Accessing the Liquid Handling Module 201 Accessing and Removing the Pre-punchers 203 Cleaning the Electrodes 205 Cleaning the Pre-punchers 206 Cleaning the Insulation Plate 207 Reinstalling the Pre-punchers 208 Reinstalling the Electrodes and the Insulation Plate 210 Reinstall the Capillary Cassette 215 Reinstall the Front Cover 215

Maintenance of the Replenishment System 216

Changing the Buffer Composition in the Replenishment System 217 The Replenishment System Will Not Be Used for Some Time 218 Further Action 219 Operations for Replenishment Maintenance 219

Changing the Air Inlet Filter 222

Accessing the Air Filter 222

Changing the Lamp 224

Removing the Existing Lamp 225 Inserting a New Lamp 227 Further Action 227

Cleaning the Instrument 228

10 Agilent 7100 Capillary Electrophoresis System User Manual

9 Parts and Materials for Maintenance 229

Overview of Maintenance Parts 230

10 Appendix 231

General Safety Information 232

Operation 232

Allgemeine Sicherheitsinformation 235 Informations générales de sécurité 238 Informazioni generali sulla sicurezza 241 Información de seguridad 244

安全に関する一般的な情報 247

一般安全信息 250

Setting Up a Test Sample Run 252

Method Parameter CE (Default Settings) 253 Method Parameter DAD 255

Chemical and Biological Safety 256 The Waste Electrical and Electronic Equipment (WEEE) Directive

(2002/96/EC) 257

Radio Interference 258 Sound Emission 259 Legal Notice 260 Agilent Technologies on Internet 261

Contents

Index 263

Agilent 7100 Capillary Electrophoresis System User Manual 11

Contents

12 Agilent 7100 Capillary Electrophoresis System User Manual

Agilent 7100 Capillary Electrophoresis System User Manual

1 Introduction

Introduction to the Agilent 7100 Capillary Electrophoresis System 14 Where to Find Information 15 What Learning Products are Available? 16

This chapter gives an introduction to the Agilent 7100 Capillary Electrophoresis System and where to find specific information or help.

Agilent Technologies

1 Introduction

Introduction to the Agilent 7100 Capillary Electrophoresis System

This handbook gives an overview of your Agilent 7100 Capillary Electrophoresis System. It is designed to get you started working with the system. We strongly advise you to make extensive use of the online help when working with the system. There you find detailed reference and task information which complements the overview given in this guide. Refer to

“Getting Help” later in this section for information on using the online help of

the ChemStation. Refer to “Where to Find Information” for help in finding information on certain tasks.

Getting Help

You can get help by choosing the Help item from the top menu. This puts you into the help index. From there you can browse through the information by selecting the appropriate jumps (underlined words). To access specific information in the help:

When you are in the Agilent ChemStation or Lab Advisor software:

• select a context-sensitive element and press F1.

When you are in the online help:

• search for a specific keyword using the search command,

• select a jump - a jump is an underlined word you can select to go to a topic

related to the word, or

• select a word or phrase with a dotted underline to see its definition. The online help also contains online information on how to use help.

14 Agilent 7100 Capillary Electrophoresis System User Manual

Where to Find Information

Tabl e 1 Where to find information

Task Refer to

Introduction 1

Where to Find Information

• Installation of the Agilent CE System,

installing the external water bath, installing the high sensitivity cell

• Setting up the Agilent CE System for

CE analysis, CE+p

• How to use the Agilent CE System in

the CE+p mode

• How to use the Agilent CE System for

capillary electrochromatography

• How to maintain the Agilent CE

System

• Setting up a sequence Online help "Understanding Your ChemStation"

• Data analysis Online help "Understanding Your ChemStation"

• Setting up a report Online help "Understanding Your ChemStation"

• Spectral library search “ChemStation Applications” handbook and online help

• Setting up customized reports “ChemStation Applications” handbook

• Safety information Safety information in “Appendix” on page 231

• Learning ChemStation concepts “Understanding Your ChemStation” handbook

• Learning the theory of the technique High Performance Capillary Electrophoresis: An

"The Core Agilent CE Instrument" “Installing the

Instrument” on page 53

Chapter “Using the Agilent 7100 Capillary

Electrophoresis System” on page 77"

“Capillary Electrophoresis, Plus High Pressure

CE+p” on page 136

“Capillary Electrochromatography, CEC” on page 148

Chapter “Maintenance” on page 189

Introduction” The CE Partner CD-ROM: “The comprehensive, interactive tool for beginners and advanced users of CE”

• Application Agilent web page (http://www.agilent.com), search for

Application notes

Agilent 7100 Capillary Electrophoresis System User Manual 15

1 Introduction

What Learning Products are Available?

The Agilent CE System is supplied with the following:

• User's Guide

• High Performance Capillary Electrophoresis: An Introduction

The Agilent ChemStation and Lab Advisor/Instrument Utilities software DVD is supplied with the following electronic documents:

• Installing Your Agilent ChemStation for LC and CE Systems

• Installing and Understanding Your Spectra Module

• Understanding Your Agilent ChemStation

• Online help

• Understanding Your Agilent ChemStation for CE Systems

16 Agilent 7100 Capillary Electrophoresis System User Manual

Agilent 7100 Capillary Electrophoresis System User Manual

2 Site Requirements and Specifications

Site Requirements 18 Physical Specifications 21 Performance Specifications 22 Anforderungen an den Aufstellungsort 23 Abmessungen und Gewicht 26 Leistungsspezifikationen 27 Exigences d’installation 28 Caractéristiques physiques 31 Caractéristiques de performance 32 Requisiti ambientali 33 Specifiche fisiche 36 Specifiche delle prestazioni 37 Requisitos de las instalaciones 38 Especificaciones físicas 41 Especificaciones de rendimiento 42

設置について 43 物理的仕様 46 性能仕様 47

场地要求 48 物理规格 51 性能规格 52

This chapter provides information on the environmental requirements, as well as physical and performance specifications.

Agilent Technologies

2 Site Requirements and Specifications

WARNING

Site Requirements

A suitable environment is important to ensure optimal performance of the 7100 Capillary Electrophoresis instrument.

Before you begin installation, check carefully that the place you have chosen meets the requirements below.

Power Consideration

The instrument power supply has wide ranging capabilities and accepts any standard line voltage in the range mentioned in Table 2 on page 21. Consequently, there is no voltage selector on the back of the detector.

The module is partially energized when switched off, as long as the power cord is plugged in.

18 Agilent 7100 Capillary Electrophoresis System User Manual

Repair or maintenance work on the module can lead to personal injuries, for example a shock hazard, when the cover is opened and the module is connected to the power.

• Make sure that it is always possible to access the power plug.

• Remove the power cable from the instrument before opening the cover.

• Do not connect the power cable to the instrument while the electronic box in the

back of the instrument is opened or side panels are removed.

Incorrect line voltage on the instrument Shock hazard or damage to your instrument can result if the device is connected to a

line voltage that is higher than specified.

• Connect your instrument to the specified line voltage.

CAUTION

Inaccessible power plug.

WARNING

In the case of an emergency it must be possible to disconnect the instrument from the power line at any time.

• Make sure the power connector of the instrument can be easily reached and unplugged.

• Provide sufficient space behind the power socket of the instrument to unplug the cable.

Power Cords

Different power cords are delivered with the instrument. The female end of all power cords is identical. It plugs into the power-input socket at the rear. The male end of each power cord is different and designed to match the wall socket of a particular country or region.

Absence of a ground connection or use of an unspecified power cord

Site Requirements and Specifications 2

Site Requirements

The absence of a ground connection or the use of an unspecified power cord can lead to an electric shock or short circuit.

• Never operate your instrument from a power outlet without a ground connection.

• Never use a power cord other than the Agilent Technologies power cord designed

for your region.

Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the

electronic components or personal injury.

• Never use cables other than the ones supplied by Agilent Technologies to ensure

proper functionality and compliance with safety or EMC regulations.

Agilent 7100 Capillary Electrophoresis System User Manual 19

2 Site Requirements and Specifications

CAUTION

Site Requirements

Bench Space

The instrument dimensions and weight mean that the instrument can be placed on almost any desk or laboratory bench. It needs an additional 2.5 cm (1.0 inch) of space on either side and approximately 8 cm (3.1 inches) in the rear for air circulation and electric connections.

If the Agilent 7100 Capillary Electrophoresis instrument is to be placed on a bench, make sure that the bench is designed to bear the weight of the system.

The instrument must be operated in a upright position.

Environment

Your instrument will work within the specifications for ambient temperatures and relative humidity described in Table 2 on page 21.

Condensation within the module Condensation will damage the system electronics.

20 Agilent 7100 Capillary Electrophoresis System User Manual

• Do not store, ship or use your module under conditions where temperature

fluctuations could cause condensation within the module.

• If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation.

Physical Specifications

NOTE

Tabl e 2 Physical specifications

Type Specification Comments

Weight 35 kg (77.2 lbs)

Site Requirements and Specifications 2

Physical Specifications

Dimensions (width × depth × height)

Line voltage 100–240 VAC ± 10% Wide-ranging capability

Line frequency 50 or 60 Hz ± 5%

Power consumption 350 VA / 300 W / 1024 BTU/h Maximum

Ambient operating temperature

Ambient non-operating temperature

Humidity below 80% at 31 °C (87.8 °F) Non-condensing

Operating altitude Up to 2000 m (6500 ft)

External cooling max. 0.5 bar (7.2 psi),

External pressure 2-12 bar (29-174 psi) Oil-free air or nitrogen

Safety standards: IEC, CSA, UL

Housing

350 x 510 x 590 mm (13.8 x 20.1 x 23.2 inches)

5–40 °C (41–104 °F)

-40 to 70°C (-40 to 158 °F)

max. 50 °C (122 °F)

Installation category II, Pollution degree 2

All materials are recyclable

Waterbath

For indoor use only

For applications run in CEC mode (Capillary Electrochromatography) or CE+p mode (Capillary Electrophoresis mode with optional usage of higher pressure), an external pressure supply of oil-free air or nitrogen with a maximum pressure of 15 bar (218 psi) can be used. A respective male adapter that fits into the instrument’s female adapter and PTFE tubing are part of the shipment.

The Agilent 7100 Capillary Electrophoresis System is designed to operate in a typical electromagnetic environment where RF transmitters, such as mobile phones, should not be used in close proximity.

Agilent 7100 Capillary Electrophoresis System User Manual 21

2 Site Requirements and Specifications

Performance Specifications

Tabl e 3 Performance specifications for the Agilent 7100 Capillary Electrophoresis System

Type Specification Comments

Safety features Extensive diagnostics, error detection and display,

current leak detection; low current limit Liquid leak sensor; safety sensors on door and cover disabling high voltage; vial sensor;

Control and data evaluation Agilent ChemStation under Windows® XP (SP3)

Communication LAN, controller-area network (CAN), RS-232C,

APG remote: ready, start, stop and shut-down signals. USB (future use). Analog out and in.

GLP features Early maintenance feedback (EMF), electronic

records of maintenance and errors

0 to1 V

22 Agilent 7100 Capillary Electrophoresis System User Manual

Anforderungen an den Aufstellungsort

WARNUNG

Eine geeignete Umgebung ist wichtig für die optimale Leistungsfähigkeit des 7100 Kapillarelektrophorese-Geräts.

Prüfen Sie sorgfältig, ob der von Ihnen gewählte Aufstellort die nachfolgenden Anforderungen erfüllt, bevor Sie mit der Installation beginnen.

Stromversorgung

Das Weitbereichsnetzteil des Geräts arbeitet mit Standard-Netzspannungen gemäß den Angaben in Tabelle 4 auf Seite 26. Daher ist an der Rückseite des Detektors kein Spannungswahlschalter vorhanden.

Auch im ausgeschalteten Zustand fließt im Modul Strom, solange das Netzkabel eingesteckt ist.

Site Requirements and Specifications 2

Anforderungen an den Aufstellungsort

Agilent 7100 Capillary Electrophoresis System User Manual 23

Die Durchführung von Reparatur- oder Wartungsarbeiten am Modul kann zu Personenschäden wie z. B. durch Stromschlag führen, wenn das Modulgehäuse geöffnet wird, während das Modul an die Netzspannung angeschlossen ist.

• Stellen Sie den freien Zugang zu den Netzkabeln sicher.

• Trennen Sie das Netzkabel vom Gerät, bevor Sie das Gehäuse öffnen.

• Schließen Sie das Netzkabel nicht an das Gerät an, während der Elektronikkasten

an der Geräterückseite geöffnet oder Seitenteile entfernt sind.

Falsche Netzspannung am Gerät Wenn das Gerät an eine höhere Spannung als spezifiziert angeschlossen wird, kann

dies zu Überspannungsschäden oder einer völligen Zerstörung des Geräts führen.

• Schließen Sie das Gerät an die angegebene Netzspannung an.

2 Site Requirements and Specifications

VORSICHT

WARNUNG

Anforderungen an den Aufstellungsort

Unzugänglicher Netzstecker. In einem Notfall muss es jederzeit möglich sein, das Gerät vom Stromnetz zu trennen.

• Stellen Sie sicher, dass der Netzstecker des Geräts leicht zugänglich ist.

• Lassen Sie hinter der Netzbuchse des Geräts genügend Platz zum Herausziehen

des Kabels.

Netzkabel

Das Gerät wird mit unterschiedlichen Netzkabeln ausgeliefert. Der weibliche Stecker ist bei jedem Netzkabel identisch. Er wird an die Netzanschlussbuchse an der Rückseite angeschlossen. Die Stecker am anderen Ende der Netzkabel sind unterschiedlich und erfüllen die Normen unterschiedlicher Länder oder Regionen.

Nicht vorhandene Erdung oder Verwendung eines nicht spezifizierten Netzkabels Bei der Verwendung des Geräts ohne Erdung oder mit einem nicht spezifizierten

Netzkabel können Stromschläge und Kurzschlüsse auftreten.

24 Agilent 7100 Capillary Electrophoresis System User Manual

• Betreiben Sie Ihr Gerät niemals an einer Spannungsquelle ohne Erdung.

• Verwenden Sie niemals ein anderes als das von Agilent zum Einsatz im jeweiligen

Land bereitgestellte Kabel.

Verwendung nicht im Lieferumfang enthaltener Kabel Die Verwendung von Kabeln, die nicht von Agilent Technologies geliefert

wurden, kann zu einer Beschädigung der elektronischen Komponenten oder zu Personenschäden führen.

• Verwenden Sie ausschließlich Originalkabel von Agilent Technologies, um eine

einwandfreie Funktion und die Einhaltung der Sicherheits- und EML-Bestimmungen zu gewährleisten.

Platzbedarf

VORSICHT

Die Abmessungen und das Gewicht des Geräts ermöglichen seine Aufstellung auf praktisch jedem Schreibtisch oder Laborarbeitstisch. Das Gerät benötigt an jeder Seite zusätzlich 2,5 cm Platz und ungefähr 8 cm an der Rückseite für die elektrischen Anschlüsse und für eine ausreichende Luftzirkulation.

Wenn das Agilent 7100 Kapillarelektrophorese System auf einem Labortisch aufgestellt werden soll, stellen Sie sicher, dass der Tisch ausreichende Tragfähigkeit für das System aufweist.

Das Gerät ist stehend zu betreiben!

Umgebungsbedingungen

Ihr Gerät arbeitet bei der Umgebungstemperatur und der relativen Luftfeuchtigkeit, die in den Spezifikationen in Tabelle 4 auf Seite 26 angegeben sind.

Kondensation im Inneren des Moduls

Site Requirements and Specifications 2

Anforderungen an den Aufstellungsort

Agilent 7100 Capillary Electrophoresis System User Manual 25

Kondensation führt zur Beschädigung der Systemelektronik.

• Vermeiden Sie die Lagerung, den Versand oder den Betrieb der Pumpe unter Bedingungen, die zu einer Kondensation in der Pumpe führen können.

• Nach einem Transport bei niedrigen Temperaturen muss das Gerät zur Vermeidung von Kondensation in der Verpackung verbleiben, bis es sich auf Raumtemperatur erwärmt hat.

2 Site Requirements and Specifications

HINWEIS

Abmessungen und Gewicht

Tabelle 4 Abmessungen und Gewicht

Typ Spezifikationen Kommentare

Gewicht 35 kg

Abmessungen (Breite × Tiefe × Höhe)

Netzspannung 100–240 V Wechselstrom ± 10 % Weitbereichsnetzteil

Netzfrequenz 50 oder 60 Hz ± 5 %

Leistungsaufnahme 350 VA / 300 W / 1024 BTU/h Maximal

zulässige Umgebungstemperatur bei Lagerung

Umgebungstemperatur bei Lagerung

Luftfeuchtigkeit unter 80 % bei 31 °C Nicht kondensierend

Betriebshöhe bis zu 2000 m

350 x 510 x 590 mm (23,2 x 13,8 x 20,1 Zoll)

5–40 °C

-40 bis 70 °C

26 Agilent 7100 Capillary Electrophoresis System User Manual

Externe Kühlung max. 0,5 bar (7,2 psi), max. 50 °C Wasserbad

Externer Druck 2-12 bar (29-174 psi) Ölfreie Luft oder Stickstoff

Sicherheitsstandards: IEC, CSA, UL

Gehäuse

Installationskategorie II, Verschmutzungsgrad 2.

Alle Materialien sind recyclebar.

Nur für den Einsatz im Innenbereich geeignet

Für Applikationen im CEC-Modus (Capillary Electrochromatography) oder CE+p-Modus (Kapillarelektrophorese-Modus mit optionaler Verwendung höheren Druckes) kann eine externe Druckversorgung mit ölfreier Luft oder Stickstoff und einem Druckmaximum von 15 bar (218 psi) verwendet werden. Ein entsprechender männlicher Adapter für den weiblichen Adapter des Geräts und PTFE-Schläuche werden mitgeliefert.

Das Agilent 7100 Kapillarelektrophorese-System ist auf den Betrieb in einer typischen elektromagnetischen Umgebung ausgelegt, in deren unmittelbarer Nähe keine HF-Sender wie z. B. Mobiltelefone verwendet werden dürfen.

Site Requirements and Specifications 2

Leistungsspezifikationen

Tabelle 5 Leistungsspezifikationen für das Agilent 7100 Kapillarelektrophorese-System

Typ Spezifikationen Kommentare

Sicherheitsvorkehrungen Umfangreiche Diagnose, Fehlererkennung und

-anzeige, Kriechstromerkennung; Grenzwert für zu niedrigen Strom Flüssigkeitslecksensor; Sicherheitssensoren an Tür und Abdeckung zur Trennung der Hochspannung; Probenflaschensensor;

Steuerung und Datenauswertung

Kommunikation LAN, CAN (Controller-Area Network), RS-232C,

GLP-Eigenschaften Frühwarnsystem für fällige Wartungen (EMF, Early

Agilent ChemStation unter Windows® XP (SP3)

APG Remote: Ready-, Start-, Stop- und Shut-down-Signale. USB (zukünftige Ver wen dung) . Analoger Ein-/Ausgang.

Maintenance Feedback), elektronische Aufzeichnung von Wartungsarbeiten und Fehlermeldungen

0 bis 1 V

Agilent 7100 Capillary Electrophoresis System User Manual 27

2 Site Requirements and Specifications

AVERTISSEMENT

Exigences d’installation

Un environnement adéquat est indispensable pour obtenir des performances optimales de l’instrument d’électrophorèse capillaire 7100.

Avant de commencer l’installation, vérifiez soigneusement que l’emplacement choisi est conforme aux exigences ci-dessous.

Remarques à propos de l’alimentation

Le module d’alimentation de l’instrument présente une tolérance importante. Il accepte ainsi n’importe quelle tension de secteur se situant dans la plage de tolérance précisée dans le Tableau 6, page 31. Ceci explique l’absence de sélecteur de tension à l’arrière du détecteur.

Le module reste partiellement activé lorsqu’il est éteint, tant que le cordon d’alimentation est branché.

Certaines opérations de réparation ou d’entretien sur le module peuvent occasionner des blessures, par exemple l’électrocution, si le capot est ouvert et que le module est branché.

•

Veillez à ce que la prise d’alimentation électrique reste à tout moment accessible.

• Débranchez le câble d’alimentation de l’instrument avant d’ouvrir le capot.

• Ne branchez pas le câble d’alimentation sur l’instrument si le boîtier

électronique à l’arrière est ouvert ou si les panneaux latéraux sont enlevés.

Tension de secteur incorrecte au niveau de l’instrument Il existe un risque d’électrocution ou de dommages à l’instrument en cas de

raccordement à une ligne d’alimentation de tension supérieure à celle spécifiée.

• Connectez l’instrument à la tension indiquée.

28 Agilent 7100 Capillary Electrophoresis System User Manual

ATTENTION

Prise d’alimentation inaccessible.

AVERTISSEMENT

En cas d’urgence, il doit être possible de débrancher à tout moment l’instrument du secteur.

• Veillez à faciliter l’accès au connecteur d’alimentation de l’instrument et à permettre de le débrancher facilement.

• Maintenez un espace suffisant derrière la prise d’alimentation de l’instrument pour pouvoir débrancher le câble.

Câbles d’alimentation

Différents câbles d’alimentation sont livrés avec l’instrument. L’extrémité femelle est la même pour tous les câbles. Elle s’enfiche dans l’embase d’alimentation située à l’arrière. L’extrémité mâle, qui se branche sur la prise de courant murale, varie selon le pays ou la région.

Absence de raccordement à la terre et utilisation d’un câble d’alimentation non adapté

Site Requirements and Specifications 2

Exigences d’installation

Agilent 7100 Capillary Electrophoresis System User Manual 29

L’absence de raccordement à la terre et l’utilisation d’un câble d’alimentation non adapté peuvent entraîner une électrocution ou un court-circuit.

• N’utilisez jamais une prise de courant sans mise à la terre.

• N’utilisez jamais de câble d’alimentation autre que le modèle Agilent

Technologies destiné à votre pays.

Utilisation de câbles non fournis L’utilisation de câbles non fournis par Agilent Technologies pourrait

endommager les composants électroniques ou provoquer des blessures.

• Pour garantir un bon fonctionnement et le respect des règles de sécurité ou de

compatibilité électromagnétique, n’utilisez jamais d’autres câbles que ceux fournis par Agilent Technologies.

2 Site Requirements and Specifications

ATTENTION

Exigences d’installation

Encombrement

Les dimensions et le poids de l’instrument permettent un placement sur quasiment n’importe quel bureau ou paillasse de laboratoire. Veillez à prévoir un dégagement de 2,5 cm de part et d’autre et d’environ 8 cm à l’arrière pour assurer la circulation de l’air et le passage des raccordements électriques

Si le système d’électrophorèse capillaire Agilent 7100 doit être placé sur une paillasse, assurez-vous que celle-ci est conçue pour supporter le poids du système.

En fonctionnement, l’instrument doit être en position verticale.

Environnement

Votre instrument fonctionnera conformément aux spécifications dans les conditions de température ambiante et d’humidité relative précisées dans le

Tableau 6, page 31.

Condensation à l’intérieur du module

30 Agilent 7100 Capillary Electrophoresis System User Manual

La condensation endommage les circuits électroniques du système.

• N’entreposez pas, ne transportez pas et n’utilisez pas votre module dans des conditions où les fluctuations de température risqueraient de provoquer de la condensation à l’intérieur du module.

• Si le module a été transporté par temps froid, ne le sortez directement pas de son emballage : laissez-le atteindre progressivement la température ambiante pour éviter toute condensation.

Caractéristiques physiques

REMARQUE

Tabl eau 6 Caractéristiques physiques

Type Caractéristique Commentaires

Poids 35 kg

Dimensions (largeur × profondeur × hauteur)

Tension secteur 100–240 V CA ± 10 % Plage de tensions étendue

Fréquence secteur 50 ou 60 Hz ± 5 %

Puissance consommée 350 VA / 300 W /

Température ambiante hors fonctionnement

Humidité Inférieure à 80 % à 31 °C Sans condensation

Altitude de fonctionnement Jusqu’à 2 000 m

Refroidissement extérieur Max. 0,5 bar, max. 50 °C Bain-marie

Pression extérieure 2-12 bar Air ou azote sans hydrocarbures

Normes de sécurité : CEI, CSA, UL

Site Requirements and Specifications 2

350 x 510 x 590 mm

1 024 BTU/h

5–40 °C

-40 à 70 °C

Catégorie d’installation II, degré de pollution 2.

Caractéristiques physiques

Maximum

Utilisation intérieure uniquement

Agilent 7100 Capillary Electrophoresis System User Manual 31

Boîtier

Tous les matériaux sont recyclables

Pour les applications fonctionnant en mode ECC (électrochromatographie capillaire) ou en mode EC+p (mode d’électrophorèse capillaire avec utilisation optionnelle de pression supérieure), une alimentation en pression extérieure d’air ou d’azote sans hydrocarbures à une pression maximale de 15 bar peut être utilisée. Un adaptateur mâle respectif adapté à l’adaptateur femelle de l’instrument et des tuyaux PTFE sont livrés avec l’instrument.

Le système d’électrophorèse capillaire Agilent 7100 est conçu pour fonctionner dans un environnement électromagnétique usuel, dans lequel des émetteurs de radiofréquences, tels que les téléphones mobiles, ne doivent pas être utilisés à proximité.

2 Site Requirements and Specifications

Caractéristiques de performance

Tabl eau 7 Caractéristiques de performance du système d’électrophorèse capillaire Agilent 7100

Ty pe C ar a c té r i st i que Commentaires

Fonctions de sécurité Diagnostic étendu, détection et affichage des

erreurs, détection du courant de fuite ; limite de courant faible Détecteur de fuites ; capteurs de sécurité sur la porte et le couvercle, avec fonction de désactivation de haute tension ; capteur de flacons ;

Commande et traitement des données

Communication Réseau local, bus CAN, RS-232C, commande

Caractéristiques BPL Maintenance prédictive (EMF), enregistrement

Agilent ChemStation sous Windows® XP (SP3)

à distance CAG : signaux ready, start, stop et shut-down. USB (utilisation ultérieure). Sortie et entrée analogiques

électronique des opérations de maintenance et des erreurs

0 à 1 V

32 Agilent 7100 Capillary Electrophoresis System User Manual

Requisiti ambientali

ATTENZIONE

Un ambiente adatto è importante per garantire le prestazioni ottimali del Sistema per elettroforesi capillare Agilent 7100.

Prima di effettuare l'installazione, controllare attentamente che il luogo scelto per l'installazione soddisfi i requisiti indicati di seguito.

Alimentazione

Lo strumento può essere utilizzato in un ampio intervallo di valori di tensione ed è in grado di accettare tensioni di linea compresi negli intervalli indicati nella Tavola 8 a pagina 36. Pertanto, nella parte posteriore del rivelatore non è presente alcun selettore di frequenza.

Quando è spento, se il cavo di alimentazione è collegato, il modulo riceve parzialmente energia.

Site Requirements and Specifications 2

Requisiti ambientali

Agilent 7100 Capillary Electrophoresis System User Manual 33

Gli interventi di riparazione o manutenzione del modulo possono provocare lesioni personali, come scosse elettriche, nel caso in cui il coperchio sia aperto e il modulo sia collegato all'alimentazione.

• Verificare che sia sempre possibile accedere alla presa di alimentazione.

• Scollegare il cavo di alimentazione dallo strumento prima di aprire il coperchio.

• Non collegare il cavo di alimentazione dello strumento se l'alloggiamento

dell'elettronica sul retro dello strumento è aperto o se i pannelli laterali sono stati rimossi.

Tensione di linea non corretta nello strumento Se gli strumenti vengono collegati a una tensione più elevata di quella prevista,

esiste il rischio di danneggiarli.

• Collegare lo strumento alla tensione di linea specificata.

2 Site Requirements and Specifications

AVVERTENZA

ATTENZIONE

Requisiti ambientali

Presa di alimentazione inaccessibile. In caso di emergenza, deve essere possibile scollegare lo strumento dalla rete elettrica

in qualsiasi momento.

• Accertarsi che il connettore di alimentazione dello strumento sia accessibile e possa essere scollegato facilmente.

• Verificare che lo spazio dietro la presa di alimentazione dello strumento sia sufficiente per consentire lo scollegamento del cavo.

Cavi di alimentazione

Lo strumento viene fornito con cavi di alimentazione diversi. L'estremità femmina è sempre uguale e deve essere introdotta nell'apposita presa di alimentazione che si trova nella parte posteriore. L'estremità maschio di ciascun cavo di alimentazione è diversa ed è progettata per adattarsi alle prese utilizzate nei vari paesi.

Assenza di messa a terra o uso di un cavo di alimentazione non specificato

34 Agilent 7100 Capillary Electrophoresis System User Manual

L'assenza del collegamento a terra o l'uso di un cavo di alimentazione non specificato può provocare scosse elettriche o cortocircuiti.

• Non utilizzare mai lo strumento con prese prive di messa a terra.

• Non utilizzare cavi di alimentazione diversi da quelli predisposti da Agilent

Technologies per i singoli paesi.

Uso di cavi non forniti L'uso di cavi non forniti da Agilent Technologies può provocare danni ai componenti

elettronici o lesioni personali.

• Utilizzare solo cavi forniti da Agilent Technologies, in modo da assicurare

il funzionamento corretto e la conformità alle norme di sicurezza o alle normative EMC.

Spazio necessario

AVVERTENZA

Le dimensioni e il peso dello strumento ne consentono il posizionamento sulla maggior parte dei banchi o tavoli di laboratorio. È necessario lasciare un ulteriore spazio di 2,5 cm su entrambi i lati e di circa 8 cm nella parte posteriore per la circolazione dell'aria.

Se si posiziona sul banco l'intero Sistema per elettroforesi capillare Agilent 7100, assicurarsi che il banco sia in grado di sostenere il peso complessivo del sistema.

Lo strumento deve essere usato in posizione verticale.

Ambiente

Lo strumento è progettato per essere usato nelle condizioni di temperatura ambientale e umidità specifiche descritte nella Tavola 8 a pagina 36.

Condensa all'interno del modulo

Site Requirements and Specifications 2

Requisiti ambientali

La condensa danneggia i componenti elettronici del sistema.

• Non immagazzinare, trasportare o utilizzare il modulo in condizioni in cui eventuali variazioni di temperatura potrebbero causare la formazione di condensa al suo interno.

• Se il modulo è stato spedito in condizioni di bassa temperatura, lasciarlo nel contenitore di imballaggio per consentirgli di raggiungere lentamente la temperatura ambiente ed evitare la formazione di condensa.

Agilent 7100 Capillary Electrophoresis System User Manual 35

2 Site Requirements and Specifications

NOTA

Specifiche fisiche

Tavo la 8 Specifiche fisiche

Tipo Specifica Commenti

Peso 35 kg

Dimensioni (larghezza × profondità × altezza)

Tensione di rete 100–240 VCA ± 10% Diversi valori di tensione accettati

Frequenza di rete 50 o 60 Hz ± 5%

Consumo elettrico 350 VA / 300 W / 1024 BTU/h Massimo

Temperatura ambiente non operativa

Umidità Inferiore all'80% a una

350 x 510 x 590 mm

5–40 °C

Da -40 a 70°C

Assenza di condensa

temperatura di 31 °C

Altitudine operativa Fino a 2.000 m

Raffreddamento esterno Max 0,5 bar , max 50 °C Bagno d'acqua

Pressione esterna 2-12 bar Aria priva di olio o azoto

Standard di sicurezza: IEC, CSA, UL

Involucri

Categoria di installazione II, grado di inquinamento 2.

Tutti i materiali sono riciclabili

Solo per uso all'interno

Per le applicazioni in modalità CEC (elettrocromatografia capillare) o CE+p (elettrocromatografia capillare con utilizzo opzionale di alta pressione), è possibile usare un sistema esterno per alimentare aria priva di olii o azoto a una pressione massima di 15 bar. Insieme al prodotto viene fornito un adattatore maschio che si inserisce nell'adattatore femmina dello strumento e tubi in PTFE.

Il Sistema per elettroforesi capillare Agilent 7100 è concepito per operare in un tipico ambiente elettromagnetico nelle cui vicinanze non devono essere presenti trasmettitori RF, come telefoni cellulari.

36 Agilent 7100 Capillary Electrophoresis System User Manual

Site Requirements and Specifications 2

Specifiche delle prestazioni

Tavo la 9 Specifiche delle prestazioni del Sistema per elettroforesi capillare Agilent 7100

Tipo Specifica Commenti

Dispositivi di sicurezza Ampia scelta di funzionalità diagnostiche,

identificazione e visualizzazione degli errori, rilevamento di perdite di corrente, limite di corrente basso Sensore per il rilevamento di perdite di liquidi, sensori di sicurezza sullo sportello e il coperchio per disabilitare l'alta tensione, sensore per vial

Controllo e valutazione dei dati

Comunicazioni Controller LAN (rete area controllore), RS-232C,

Funzioni GLP Avviso di manutenzione preventiva (Early

Agilent ChemStation per Windows® XP (SP3)

APG remoto: segnali di pronto, avvio, arresto e interruzione (ready, start, stop e shut-down) USB (per un uso futuro). Uscita e ingresso analogici

maintenance feedback, EMF), registro elettronico della manutenzione e degli errori

Da 0 a 1 V

Agilent 7100 Capillary Electrophoresis System User Manual 37

2 Site Requirements and Specifications

ADVERTENCIA

Requisitos de las instalaciones

Es importante disponer de un entorno adecuado para lograr un óptimo rendimiento del sistema de electroforesis capilar 7100.

Antes de comenzar la instalación, compruebe minuciosamente que el lugar elegido cumple los requisitos citados a continuación.

Consideraciones sobre la corriente

La fuente de alimentación del instrumento tiene un amplio rango de capacidades y admite cualquier voltaje de línea estándar en el rango especificado en la Tabla 10 de la página 41. Por lo tanto, no hay ningún selector de voltaje en la parte posterior del detector.

Mientras el cable de alimentación esté conectado, el módulo sigue recibiendo corriente aunque esté apagado.

38 Agilent 7100 Capillary Electrophoresis System User Manual

Las tareas de reparación o mantenimiento del módulo entrañan riesgos de lesiones personales, como descargas, si se abre la cubierta del instrumento mientras éste permanece conectado a la corriente.

• Asegúrese de poder acceder siempre al enchufe de corriente.

• Retire el cable de corriente del instrumento antes de abrir la cubierta del módulo.

• No conecte el cable de alimentación al instrumento si la caja de la electrónica

de la parte trasera está abierta o si se han retirado los paneles laterales.

Voltaje de línea incorrecto en el instrumento Si el instrumento se conecta a un voltaje superior al especificado, existe peligro

de electrocución o de daños en los instrumentos.

• Conecte el instrumento al voltaje de línea especificado.

PRECAUCIÓN

Enchufe de alimentación inaccesible.

ADVERTENCIA

En caso de emergencia, debe ser posible desconectar el instrumento de la red inmediatamente.

• Asegúrese de que es posible alcanzar y desconectar fácilmente el enchufe del instrumento.

• Deje espacio suficiente por detrás de la toma de corriente del instrumento para desconectar el cable.

Cables de alimentación

El instrumento se entrega con diferentes cables de alimentación. Los terminales hembra de todos los cables de alimentación son idénticos. Se introducen en el conector de entrada de corriente de la parte posterior. El terminal macho de cada cable de alimentación es diferente y está diseñado acorde con los enchufes de cada país o región.

Conexión a tierra inexistente o uso de un cable de alimentación no especificado

Site Requirements and Specifications 2

Requisitos de las instalaciones

Agilent 7100 Capillary Electrophoresis System User Manual 39

La falta de toma de tierra y el uso de un cable de alimentación no especificado pueden provocar electrocución o cortocircuitos.

• No utilice nunca el instrumento con una toma de corriente sin conexión a tierra.

• No utilice nunca un cable de alimentación distinto del suministrado por Agilent

Technologies diseñado para su región.

Uso de cables no suministrados El uso de cables no suministrados por Agilent Technologies puede producir lesiones

personales o daños en los componentes electrónicos.

• Para asegurar un funcionamiento correcto y el cumplimiento de los reglamentos de

seguridad o de compatibilidad electromagnética, no utilice nunca cables distintos de los suministrados por Agilent Technologies.

2 Site Requirements and Specifications

PRECAUCIÓN

Requisitos de las instalaciones

Espacio en el banco

Las dimensiones y el peso del instrumento facilitan su colocación sobre prácticamente cualquier mesa de laboratorio. Deben dejarse unos 2,5 cm libres a cada lado y unos 8 cm en la parte posterior para la circulación del aire y las conexiones eléctricas.

En caso de colocar el Instrumento de Electroforesis Capilar Agilent 7100 en un banco, confirme que éste pueda soportar el peso del sistema.

El instrumento se debe utilizar en posición vertical.

Entorno

El instrumento funcionará dentro de las especificaciones de temperatura ambiente y humedad relativa descritas en la Tabla 10 de la página 41.

Condensación dentro del módulo La condensación dañará la electrónica del sistema.

40 Agilent 7100 Capillary Electrophoresis System User Manual

• No guarde, traslade ni utilice el módulo bajo condiciones en las que las

fluctuaciones de temperatura pudieran provocar condensación dentro del módulo.

• Si el traslado del módulo se realizó bajo condiciones ambientales frías, manténgalo en su caja hasta que alcance lentamente la temperatura ambiente, para evitar problemas de condensación.

Especificaciones físicas

NOTA

Tabl a 10 Especificaciones físicas

Tipo Especificaciones Comentarios

Peso 35 kg

Site Requirements and Specifications 2

Especificaciones físicas

Dimensiones (anchura × profundidad × altura)

Voltaje de línea 100–240 V CA ± 10% Capacidad de amplio rango

Frecuencia de línea 50 o 60 Hz ± 5%

Consumo de corriente 350 VA / 300 W / 1024 BTU/h Máximo

Temperatura ambiente operativa

Temperatura ambiente no operativa

Humedad Por debajo del 80% a 31 °C Sin condensación

Altitud operativa Hasta 2000 m

Refrigeración externa máx. 0,5 bares, máx. 50 °C Baño María

Presión externa 2-12 bares Aire o nitrógeno sin aceite

Estándares de seguridad: IEC, CSA, UL

Carcasa

350 x 510 x 590 mm

5–40 °C

De -40 a 70 °C

Categoría de instalación II, grado de contaminación 2.

Todos los materiales son reciclables

Sólo para uso dentro de edificios

Para aplicaciones en modo CEC (cromatografía electrocapilar) o CE+p (electroforesis capilar con uso optativo de alta presión), puede emplearse una fuente externa de presión, a base de aire o nitrógeno sin aceite, a una presión máxima de 15 bares. A la entrega se suministra también el correspondiente adaptador macho que encaja con el hembra y los tubos de PTFE del instrumento.

El sistema de electroforesis capilar Agilent 7100 está diseñado para funcionar en un entorno electromagnético normal, en cuya proximidad no deben usarse transmisores de RF como, por ejemplo, teléfonos móviles.

Agilent 7100 Capillary Electrophoresis System User Manual 41

2 Site Requirements and Specifications

Especificaciones de rendimiento

Tabl a 11 Especificaciones de rendimiento del sistema de electroforesis capilar Agilent 7100

Tipo Especificaciones Comentarios

Características de seguridad Diagnósticos completos, detección y presentación

de errores, detección de fugas, límite inferior de corriente. Sensor de fuga de líquidos, sensores de seguridad en la puerta y la tapa para desactivar el alto voltaje, sensor de viales.

Control y evaluación de datos Agilent ChemStation en Windows® XP (SP3)

Comunicaciones LAN, red de área de controlador (CAN), RS-232C,

mando a distancia APG: señales de "ready", "start", "stop" y "shut-down". USB (uso futuro). Entrada y salida analógicas.

Características de GLP Mantenimiento preventivo asistido (EMF),

registros electrónicos de mantenimiento y errores

De 0 a 1 V

42 Agilent 7100 Capillary Electrophoresis System User Manual

設置について

警告

7100 キャピラリ電気泳動装置が最適な性能で動作するためには、適切な環境に設置する必要があります。

設置作業を開始する前に、設置場所が以下の要件を満たしているかどうかを十分に確認してください。

電源について

本機器の電源は広範囲にわたる入力電圧に対応しており、46 ページ図  表 12 に記載された範囲の標準的な入力電圧で使用できます。したがって、装置背面に電圧スイッチはありません。

電源コードが差し込まれている限り、電源を切っていても、モジュールは部分的に通電しています。

Site Requirements and Specifications 2

設置について

モジュールの修理作業時や保守作業時に人身障害に至る恐れがあります。たとえば、カバーが開いていてモジュールが電源に接続されている場合の感電などです。

• 電源コネクタに常にアクセスすることが可能か確認します。

• カバーを開ける前に、装置から電源ケーブルを取り外します。

• 装置背面の電子ボックスが開いているときやサイド・パネルが取り外されているときに、電源ケーブルを装置に接続しないでください。

装置に対する不適正な入力電圧

仕様より高い入力電圧に接続すると、装置が衝撃を受けたり損傷したりすることがあります。

• 使用する装置は、指定された入力電圧に接続してください。

Agilent 7100 Capillary Electrophoresis System User Manual 43

2 Site Requirements and Specifications

注意

警告

設置について

電源コネクタに手が届かない緊急時には、いつでも電源から装置を切り離せるようにする必要がありま

す。

• 装置の電源コネクタに簡単に手が届き、抜くことができるかどうか確認します。

• ケーブルを抜くことができるように、装置電源ソケットの後ろには十分な空間を確保してください。

電源コード

本装置には各種の電源コードが付属しています。すべての電源コードのメス型側は同一の形です。電源コードのメス型側は、装置背面の電源ケーブルコネクタに差し込みます。電源コードのオス型側はコードによって異なり、各使用国または各地域のコンセントに合わせて設計されています。

接地していない場合または指定外の電源コードの使用

接地していない場合や指定外の電源コードを使用した場合、感電や回路の短絡に至ることがあります。

• 接地していない電源を使用して本装置を動作させないでください。

• また、使用する地域に合わせて設計された電源コード以外は、決して使用しないでください。

指定外ケーブルの使用

アジレントによって供給されたケーブル以外を使用すると、電子部品の損傷や人体に危害を及ぼすことがあります。

• 安全基準または EMC 規格への準拠を保証できるよう、アジレント製以外のケーブルは使用しないでください。

44 Agilent 7100 Capillary Electrophoresis System User Manual

作業台スペース

注意

本装置の寸法と重量は、ほぼすべての机や作業台に設置できるように設計されています。空気の循環と電源ケーブル接続のために、本装置の両側に 2.5 cm、背面に約 8 cm の空間が必要です。

作業台に Agilent 7100 装置を設置する場合、作業台が装置の重量に耐えるように設計されているか確認します。

本装置は必ず垂直に設置してください。

環境条件

本装置は、46ページ図表12に記載されている周囲温度および相対湿度の仕様の範囲内で動作します。

モジュール内の結露結露によってシステムの電気回路が損傷することがあります。

• 温度変化によってモジュール内に結露が発生する可能性がある環境条件では、モジュールの保管、輸送、または使用を行わないでください。

Site Requirements and Specifications 2

設置について

• 寒冷な天候下でモジュールが出荷された場合は、結露が発生しないように、オートサンプラを梱包箱に入れたままゆっくり室温まで上げてください。

Agilent 7100 Capillary Electrophoresis System User Manual 45

2 Site Requirements and Specifications

注記

物理的仕様

表 12 物理的仕様

タイプ仕様説明

重量 35 kg

寸法 ( 幅 × 奥行き × 高さ )

入力電圧 100240 VAC ± 10% 広範囲の電圧に対応

電源周波数 50 または 60 Hz ± 5 %

消費電力 350 VA / 300W/1024B

動作周囲温度 540 ℃

保管周囲温度 -40 ～ 70 ℃

湿度 31 ℃で 80 % 以下結露なし

使用高度最大 2,000 m

外部冷却最大 0.5 bar、最高 50 ℃水槽

外部圧力 2-12 bar オイルフリーの空気または窒素

安全規格： IEC、 CSA、UL

ハウジング

350 x 510 x 590 mm

TU/ 時

設置クラス II、汚染度 2

全材料リサイクル可

最大

室内使用専用

CEC ( キャピラリ電気クロマトグラフィ ) モードまたは CE+p ( キャピラリ電気泳動、高圧使用オプション適用 ) モードでアプリケーションを使用する場合、最大 15 bar のオイルフリーの空気または窒素を外部装置から供給できます。本装置のメス型アダプタおよび PTFE チューブに合わせたオス型アダプタが付属しています。

Agilent 7100 電気泳動システムは、標準的な電磁環境で動作するように設計されているため、近くで携帯電話などの無線送信機を使用しないでください。

46 Agilent 7100 Capillary Electrophoresis System User Manual

Site Requirements and Specifications 2

性能仕様

表 13 Agilent 7100 システムの性能仕様

タイプ仕様説明

安全性機能豊富な診断機能、エラーの検出と表示、漏

電検出、電流の下限値漏水センサ、高電圧を無効化する安全センサ ( 扉およびカバーに搭載 )、バイアル・センサ

性能仕様

コントロールおよびデータの評価

通信 LAN、コントローラ・エリア・ネットワーク

GLP 機能アーリーメンテナンス・フィードバック

Agilent ChemStation (Windows® XP SP3 対応 )

(CAN)、RS-232C、APG リモート : Ready、 Start、Stop、Shut-down の各シグナル。 USB ( 将来使用 )。アナログ出力およびアナログ入力。

(EMF) 機能、メンテナンスとエラーの電子記録

0 ～ 1 V

Agilent 7100 Capillary Electrophoresis System User Manual 47

2 Site Requirements and Specifications

警告

场地要求

为了确保达到 7100 毛细管电泳仪的最佳性能，务必选择适合的环境。

开始安装之前，请仔细检查所选场地是否满足下列要求。

电源要求

仪器电源适合较宽的电压范围，可承受第 51 页的表 14 中所列范围内的任何标准线路电压。因此，检测器的背面没有电压选择器。

只要未拔掉电源线，即使在关闭部件后，部件仍会部分带电。

在部件盖打开且部件处于通电状态时，维修或维护部件可能会造成人身伤害

（如电击危险）。

• 请确保始终可以拔插电源插头。

• 在打开部件盖之前，请拔掉仪器的电源电缆。

• 当仪器背面的电子盒打开或侧板被拆下时，请勿连接仪器的电源电缆。

连接至仪器的线路电压不正确

如果设备连接到的线路电压超过了规定电压，则会造成电击危险或仪器损坏。

• 请将仪器连接至规定的线路电压。

48 Agilent 7100 Capillary Electrophoresis System User Manual

电源线

小心

警告

Site Requirements and Specifications 2

场地要求

电源插头不可插拔。

遇到紧急情况时，必须能够随时断开仪器与电源线的连接。

• 请确保仪器的电源插头易于插入和拔出。

• 仪器电源插座后面要有足够空间，以便拔出电缆。

仪器随附有多种电源线。所有电源线的母接头均相同。母接头插入到后部的电源插座中。每根电源线的公接头均不同，是为了与特定国家或地区的墙上插座相匹配而专门设计的。

没有接地连接或没有使用规定的电源线

没有接地连接和没有使用规定的电源线可能导致电击或短路。

• 切勿将仪器连接到没有接地连接的电源插座中。

• 除安捷伦科技为使用地区设计的电源线外，切勿使用其他电源线。

使用非随附的电缆

使用非安捷伦科技提供的电缆会导致电子元件受损或人员受伤。

• 为确保功能正常并符合安全规定或 EMC 规定，切勿使用不是由安捷伦科技提供的电缆。

Agilent 7100 Capillary Electrophoresis System User Manual 49

2 Site Requirements and Specifications

小心

场地要求

工作台位置

就仪器的尺寸和重量而言，仪器几乎可置于任何桌子或实验室工作台上。为了便于空气流通和电路连接，需要在仪器左右两侧各留出 2.5 cm 的空间，并在背面留出大约 8cm的空间。

如果要将 Agilent 7100 仪器放置在工作台上，请确保此工作台能够承受该系统的重量。

仪器必须竖直放置。

环境

仪器的工作环境应符合第 51 页的表 14 中所规定的环境温度和相对湿度要求。

部件内的冷凝

如发生冷凝，将导致系统电子元件受损。

• 温度波动可能会导致部件内发生冷凝，请不要在这种条件下贮存、运输或使用部件。

• 如果在寒冷天气条件下运输部件，不要马上开箱，应等到部件在运输箱内缓慢升至室温后再开箱，以此避免发生冷凝。

50 Agilent 7100 Capillary Electrophoresis System User Manual

物理规格

注意

Site Requirements and Specifications 2

表 14 物理规格

类型规格注释

重量

尺寸

（宽 × 长 × 高）

线路电压

线路频率 50 Hz 或 60 Hz ± 5%

耗电量

操作环境温度

35 kg

350 x 510 x 590 mm

–240 VAC ± 10%

100

350 VA / 300 W / 1024 BT U/h

–40 °C

较宽的适用范围

最大

物理规格

非操作环境温度

湿度 31 °C 时低于 80% 不冷凝

操作海拔高度最高 2000 m

外部冷却最大 0.5 bar，最高 50 °C 水浴

外部压力

安全标准：IEC、 CSA、 UL

外壳

对于在

CEC

模式（毛细管电色谱法）或压力）下的应用，则可以使用可提供最大压力设备。

随附适合于仪器母接头的相应公接头以及

-40 °C 至 70 °C

（-40 °F 至 158 °F）

2-12 bar

安装类别 II，污染程度 2。

所有材料均可回收利用

CE+p

模式（毛细管电泳模式且可选用较高

15 bar

无油空气或氮气

仅限于室内使用

的无油空气或氮气的外部压力

PTFE

管。

Agilent 7100 毛细管电泳系统可以在典型电磁环境下使用，在这种环境下不应近距离使用射频发射器，例如移动电话。

Agilent 7100 Capillary Electrophoresis System User Manual 51

2 Site Requirements and Specifications

性能规格

表 15 Agilent 7100 系统的性能规格

类型规格注释

安全功能全面诊断、故障检测和显示、漏电检测；电

流下限液体泄漏传感器；安全传感器（位于门和盖上，用于禁用高电压）；样品瓶传感器；

控制和数据评估在 Windows XP (SP3) 下运行的 Agilent

ChemStation

通讯 LAN，控制器局域网 (CAN)， RS-232C，

APG 遥控：就绪、启动、停止和关机信号。 USB （将来使用）。

模拟输出和输入。

GLP 功能维护信息预报 (EMF)，维护和故障的电子记

录

0 V 至 1V

52 Agilent 7100 Capillary Electrophoresis System User Manual

Agilent 7100 Capillary Electrophoresis System User Manual

3 Installing the Instrument

Unpacking the Agilent CE Instrument 54 Installing the Instrument 57

In this chapter you will find information on

• how to unpack the Agilent CE instrument

• how to set up the Agilent CE instrument

Information on how to install the Agilent ChemStation software or on how to install the Lab Advisor/Instrument Utilities software can be found in separate documentation located on the respective software DVD.

Agilent Technologies

3 Installing the Instrument

CAUTION

Unpacking the Agilent CE Instrument

Damaged Packaging

If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment.

"Defective on arrival" problems If there are signs of damage, please do not attempt to install the module. Inspection by

Agilent is required to evaluate if the instrument is in good condition or damaged.

• Notify your Agilent sales and service office about the damage.

• An Agilent service representative will inspect the instrument at your site and

initiate appropriate actions.

54 Agilent 7100 Capillary Electrophoresis System User Manual

Unpacking the CE Instrument

The Agilent CE instrument is heavy and should be carried by two people.

1 Use the recessed handles on both sides to carry the Agilent CE instrument. 2 Open the box in which the Agilent CE instrument was shipped. 3 Pull the Agilent CE instrument out of the box and position it in a suitable

location.

Unpacking the Agilent CE Instrument

Shipment container

7100 instrument

Accessory boxes

Figure 1 Unpacking the Agilent CE instrument

Installing the Instrument 3

Agilent 7100 Capillary Electrophoresis System User Manual 55

Delivery Checklist

Ensure all parts and materials have been delivered with the instrument. Please report missing or damaged parts to your local Agilent Technologies sales and service office.

Accessories

The accessories shipped with the Agilent CE instrument are in additional boxes on right and left in the instrument box. Check that you have received everything using Table 16.

3 Installing the Instrument

Unpacking the Agilent CE Instrument

Tabl e 16 Accessories

Description Part number Quantity

HPCE startup capillary kit G7100-68700 1

Detector interface, green, 50 µm G7100-60210 1

Detector interface, red, 50 µm, BF3 G7100-60230 1

Accessory kit G7100-68705 1

– Coupler 0100-1633 2

– O-ring 25.12-mm-id 1.78-mm-XSECT-DIA 0905-1163 1

– Container 1540-0307 1

– Fuse T6.3AH, 250 VAC 2110-1018 2

– Fuse T6.3AL, 250 VAC 2110-0623 2

– Fuse T3.15AL, 250 VAC 2110-1417 4

– Air filter 3150-0619 1

– Tubing assembly 5063-6527 1

– Screwdriver, slot shaft 8710-2607 1

– Screwdriver, Pozidriv shaft 8710-0899 1

– 12 mm socket wrench 8710-2076 1

Lift tubing assy G1600-67003 1

Vial crimp/snap polypropylene 1 mL 100/PK 5182-0567 1

Snap caps, polyurethane 100/PK 5181-1512 1

Cable, remote 5061-3378 1

CE catalog 5988-6616EN 1

LAN cable (crossover) 5023-0203 1

Bottle, 500 mL, electrolyte 9300-1748 2

HPCE-Primer G7100-90001 1

CE System User’s Guide G7100-90000 1

Glass filter, solvent inlet 5041-2168 1

Electrode alignment assy G7100-60107 1

56 Agilent 7100 Capillary Electrophoresis System User Manual

Installing the Instrument

WARNING

Physical Connection

Module is partially energized when switched off, as long as the power cord is plugged in.

Repair or maintenance work on the module can lead to personal injuries, for example a shock hazard, when the cover is opened and the module is connected to the power.

• Ensure that line power cord is disconnected before setting up your Agilent CE

instrument.

In general you have to go through the following steps. 1 Unpack the Agilent CE instrument and position it in a suitable location on

your bench, see “Unpacking the Agilent CE Instrument” on page 54.

2 Connect the cables at the rear of the Agilent CE instrument. 3 Install the bottles in the replenishment system, see “Preparing the

Replenishment System if Needed” on page 111.

Installing the Instrument 3

Installing the Instrument

Agilent 7100 Capillary Electrophoresis System User Manual 57

3 Installing the Instrument

Installing the Instrument

Connecting Cables

The Agilent CE instrument has the following connectors on the rear.

• All electrical connectors, such as line power, LAN connector or fuses, are

located on the right-hand side of the electronic box on the back of the instrument.

• All connectors for liquids and external pressure supply are located on the

left-hand side of the electronic box.

1 Ensure that the line power switch on the front of the instrument is OFF. 2 Connect the following cables as shown in Table 2 and in Figure 2.

a One power cable b LAN cable, which connects the Agilent CE instrument directly to the

computer with the Agilent ChemStation installed or to the local network.

For a description of remaining items refer to the Hardware section.

• For more information on cable connection to 3

out, please refer to “Hardware Information” on page 171.

party detectors or analog

58 Agilent 7100 Capillary Electrophoresis System User Manual

Electrical connectors

Connectors for liquids and external pressure

LAN connector

Line power

Liquid cooling

External pressure connector

Drainage

in connector

out connector

supply

Installing the Instrument 3

Installing the Instrument

Agilent 7100 Capillary Electrophoresis System User Manual 59

Figure 2 Connections on the rear of the instrument

3 Installing the Instrument

Installing the Instrument

60 Agilent 7100 Capillary Electrophoresis System User Manual

Agilent 7100 Capillary Electrophoresis System User Manual

4 LAN Configuration

This chapter provides information on connecting the CE instrument to the Agilent ChemStation PC.

Agilent Technologies

4 LAN Configuration

What You Have to Do First

The instrument has an on-board LAN communication interface. Please review the following options:

1 The instrument will be connected to the LAN: Ask your administrator to

provide you with an IP address, a subnet mask address and a gateway address. Proceed with manual configuration (see “Manual

Configuration” on page 71). You will have to initially establish a LAN

connection using the default settings of the instrument and a crossover LAN cable (see Table 19 on page 68).

2 The instrument and PC will be set up locally via a crossover cable or a hub:

No addresses are needed. Proceed with establishing a LAN connection using the default settings of the instrument (see Table 19 on page 68).

3 Identification of the instrument in your LAN by MAC address: If you plan to

let a Bootp service (see “Agilent Bootp Service” on page 75) assign an IP address please continue with locating the Media Access (MAC) address and proceed with the respective initialization modes in “Initialization Mode

Selection” on page 65 and the installation instruction in the "Installing your

ChemStation for LC and CE" manual. Ask your administrator to provide you with an IP address, a subnet mask address and a gateway address.

In addition to LAN communication, the instrument allows serial communication access via the RS 232 cable to the COM port of the PC. However, the data transfer rate would be too low for running the instrument at full performance. This alternative data connection is only meaningful if the Lab Advisor software is for example used for troubleshooting reasons (see

“Agilent Lab Advisor Software” on page 169).

Please see “Starting Your Computer and the Agilent ChemStation

Software” on page 81 for information on configuration of the connection

settings of the ChemStation software.

62 Agilent 7100 Capillary Electrophoresis System User Manual

TCP/IP Parameter Configuration

To operate properly in a network environment, the LAN interface must be configured with valid TCP/IP network parameters. These parameters are:

• IP address

• Subnet mask

• Default gateway

The TCP/IP parameters can be configured by the following methods:

• by automatically requesting the parameters from a network-based BOOTP

service (using the so-called bootstrap protocol), see “Agilent Bootp

Service” on page 75

• by manually setting the parameters using Telnet

The LAN interface differentiates between several initialization modes. The initialization mode (abbreviation: 'init mode') defines how to determine the active TCP/IP parameters after power-on. The parameters may be derived from a Bootp cycle, non-volatile memory or initialized with known default values. The initialization mode is selected by the configuration switch, see

Table 17 on page 64.

LAN Configuration 4

TCP/IP Parameter Configuration

Agilent 7100 Capillary Electrophoresis System User Manual 63

4 LAN Configuration

NOTE

Configuration Switch

The configuration switch can be accessed on the right rear side of the instrument. The 7100 CE is shipped with switches 7 and 8 set to ON. This means that the instrument is set to a fixed default IP: 192.168.254.11

To perform any LAN configuration, SW1 and SW2 must be set to OFF.

Tabl e 17 Factory default settings

Initialization ('init') mode Using default, switches 7 and 8 set to ON.

Link configuration Speed and duplex mode determined by

auto-negotiation

64 Agilent 7100 Capillary Electrophoresis System User Manual

Initialization Mode Selection

The following initialization (init) modes are selectable:

Tabl e 18 Initialization mode switches

Bootp

When the initialization mode "Bootp" is selected, the 7100 CE tries to download the parameters from a Bootp server. The parameters obtained become the active parameters immediately. They are not stored in the non-volatile memory of the 7100 CE. Therefore, the parameters are lost with the next power cycle of the 7100 CE.

LAN Configuration 4

Initialization Mode Selection

SW6 SW7 SW8 Init mode

OFF OFF OFF Bootp

OFF OFF ON Bootp & Store

OFF ON OFF Using Stored

OFF ON ON Using Default

Figure 3 Bootp (principle)

Agilent 7100 Capillary Electrophoresis System User Manual 65

4 LAN Configuration

NOTE

Initialization Mode Selection

Bootp & Store

When "Bootp & Store" is selected, the parameters obtained from a Bootp server become the active parameters immediately. In addition, they are stored in the non-volatile memory of the 7100 CE. Thus, after a power cycle they are still available. This enables a kind of "Bootp once" configuration of the 7100 CE.

Example: The user may not want to have a Bootp server be active in his network all the time. But on the other hand, he may not have any other configuration method than Bootp. In this case he starts the Bootp server temporarily, powers on the 7100 CE using the initialization mode "Bootp & Store", waits for the Bootp cycle to be completed, closes the Bootp server and powers down the 7100 CE. Then, he selects the initialization mode "Using Stored" and powers on the 7100 CE again. From now on, he is able to establish the TCP/IP connection to the 7100 CE with the parameters obtained in that single Bootp cycle.

Figure 4 Bootp & Store (principle)

Use the initialization mode "Bootp & Store" carefully, because writing to the non-volatile memory takes time. Therefore, if the 7100 CE is to obtain its parameters from a Bootp server every time it is powered on, the recommended initialization mode is "Bootp"!

66 Agilent 7100 Capillary Electrophoresis System User Manual

LAN Configuration 4

NOTE

Initialization Mode Selection

Using Stored

When initialization mode "Using Stored" is selected, the parameters are taken from the non-volatile memory of the instrument. The TCP/IP connection will be established using these parameters. The parameters were configured previously by one of the described methods.

Figure 5 Using Stored (principle)

Using Default

When “Using Default” is selected, the factory default parameters are used instead. These parameters enable a TCP/IP connection to the LAN interface without further configuration, see Table 19 on page 68.

A direct connection of a computer and the 7100 instrument is the entry point to change the stored parameters of the instrument. The default parameters of the 7100 instrument are permanently stored and cannot be changed. Using them, this allows you to establish a LAN connection at any time when the init mode “Using Default” is configured.

Figure 6 Using Default (principle)

Using the default address in your local area network may result in network problems. Take care and change it to a valid address immediately unless you plan a direct LAN connection to stand-alone PC.

Agilent 7100 Capillary Electrophoresis System User Manual 67

4 LAN Configuration

NOTE

Initialization Mode Selection

Tabl e 19 Using Default parameters

IP address: 192.168.254.11

Subnet mask: 255.255.255.0

Default gateway Not specified

Since the default IP address is a so-called local address, it will not be routed by any network device. Thus, the PC and the CE instrument must be in the same subnet.

The user may open a Telnet session using the default IP address and change the parameters stored in the non-volatile memory of the CE instrument. He may then close the session, select the initialization mode "Using Stored", power-on again and establish the TCP/IP connection using the new parameters.

When the 7100 CE instrument is wired to the PC directly (for example using a cross-over cable or a local hub), that is separate from the local area network, the user may simply keep the default parameters to establish the TCP/IP connection.

In the "Using Default" mode, the parameters stored in the memory of the 7100 Capillary Electrophoresis instrument are not cleared automatically. If not changed by the user, they are still available when you switch back to the mode "Using Stored".

68 Agilent 7100 Capillary Electrophoresis System User Manual

Link Configuration Selection

The LAN interface supports 10 or 100 Mbps operation in full or half-duplex modes. In most cases, full-duplex is supported when the connecting network device - such as a network switch or hub - supports IEEE 802.3u auto-negotiation specifications.

When connecting to network devices that do not support auto-negotiation, the LAN interface will configure itself for 10 or 100-Mbps half-duplex operation.

For example, when connected to a non-negotiating 10-Mbps hub, the LAN interface will be automatically set to operate at 10-Mbps half-duplex.

If the CE instrument is not able to connect to the network through auto-negotiation, you can manually set the link operating mode using link configuration switches on the CE instrument.

Tabl e 20 Link configuration switches

LAN Configuration 4

Link Configuration Selection

SW3 SW4 SW5 Link configuration

OFF - - Speed and duplex mode determined by

auto-negotiation

ON OFF OFF Manually set to 10 Mbps, half-duplex

ON OFF ON Manually set to 10 Mbps, full-duplex

ON ON OFF Manually set to 100 Mbps, half-duplex

ON ON ON Manually set to 100 Mbps, full-duplex

Agilent 7100 Capillary Electrophoresis System User Manual 69

4 LAN Configuration

Storing the Settings Permanently with Bootp

If you want to change parameters of the CE instrument using Bootp follow the instructions below.

1 Turn off the CE instrument. 2 Change the CE instrument's settings for the configuration switch to “Bootp

& Store” mode, see Table 18 on page 65.

3 Start the Agilent Bootp Service (see “Agilent Bootp Service” on page 75)

and open its window.

4 If required, modify the parameters for the CE instrument according to your

needs using the existing configuration.

5 Press OK to exit the Bootp manager. 6 Now turn on the CE instrument and view the Bootp server window. After

some time the Agilent Bootp Service will display the request from the LAN interface. The parameters are now stored permanently in the non-volatile memory of the CE instrument.

7 Close the Agilent Bootp Service and turn off the CE instrument. 8 Change the settings of the CE instrument’s configuration switch to “Using

Stored” mode, see Table 18 on page 65.

9 Power cycle the CE instrument. The CE instrument can be accessed now via

LAN without the Agilent Bootp Service, refer to “Agilent Bootp Service” on page 75.

70 Agilent 7100 Capillary Electrophoresis System User Manual

Manual Configuration

Manual configuration only alters the set of parameters stored in the non-volatile memory of the CE instrument. It never affects the currently active parameters. Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, if the initialization mode selection switches permit this.

LAN Configuration 4

Manual Configuration

Figure 7 Manual configuration (principle)

Agilent 7100 Capillary Electrophoresis System User Manual 71

4 LAN Configuration

Manual Configuration

With Telnet

Whenever a TCP/IP connection to the 7100 CE instrument is possible (TCP/IP parameters set by any method), the parameters may be altered by opening a Telnet session. Please refer to “Using Default” on page 67 for establishing an initial LAN connection.

1 Open the system (DOS) prompt window by clicking the Windows START

2 Type the following after the system (DOS) prompt:

button and select "Run...". Type "cmd" and press OK.

•

c:\>telnet <IP address>

•

c:\>telnet <host name>

Figure 8 Telnet - starting a session

where <IP address> may contain the assigned address from a Bootp cycle, or the default IP address (see “Configuration Switch” on page 64).

If the connection is established successfully, the instrument responds with the following:

Figure 9 A connection to the instrument has been made

72 Agilent 7100 Capillary Electrophoresis System User Manual

3 Type

and press [Enter] to see the available commands.

Figure 10 Telnet commands

Tabl e 21 Telnet commands

Val ue De scrip tion

LAN Configuration 4

Manual Configuration

? Displays syntax and descriptions of commands

/ Displays current LAN settings

ip <x.x.x.x> Sets new IP address

sm <x.x.x.x> Sets new subnet mask

gw <x.x.x.x> Sets new default gateway

exit Exits shell and saves all changes

4 To change a parameter follows the style:

• parameter value, for example:

ip 134.40.27.230

Then press [Enter]; parameter refers to the configuration parameter you are defining, and value refers to the definitions you are assigning to that parameter. Each parameter entry is followed by a carriage return.

Agilent 7100 Capillary Electrophoresis System User Manual 73

4 LAN Configuration

NOTE

Manual Configuration

5 Use “/” and press [Enter] to list the current settings.

Figure 11 Telnet - current settings in "Using Stored" mode

6 Change the IP address (in this example 134.40.27.99) and type “/” to list the

Information about the LAN interface MAC address, initialization mode Initialization mode is Using Stored active TCP/IP settings

TCP/IP status - here ready connected to a PC with controller software

(for example Agilent ChemStation), here not connected

current settings.

If the initialization mode switch is changed now to “Using Stored” mode, the instrument will take over the stored settings when the instrument is re-booted. In the example above, it would be 134.40.27.99.

74 Agilent 7100 Capillary Electrophoresis System User Manual

Agilent Bootp Service

Agilent Bootp Service provides central administration of IP addresses for Agilent CE instruments in a LAN. The service runs on the instrument LAN PC, which must be running a TCP/IP network protocol and cannot run a DHCP server.

When an instrument is powered on, a LAN interface in the instrument sends a request for an IP address or host name and provides its hardware address as an identifier. The request may continue for up to 5 minutes. The Agilent Bootp Service answers this request and passes a previously defined IP address and host name associated with the hardware address to the requesting instrument.

When the instrument receives its IP address and host name, it stops sending the request. It maintains the IP address as long as it is powered on. Powering down the instrument causes it to lose its IP address, so the Agilent Bootp Service must be started. Since the Agilent Bootp Service runs in the background, the instrument will receive its IP address once it is powered up.

LAN Configuration 4

Agilent Bootp Service

Detailed documentation on installation and the usage of the Agilent Bootp Service can be found on your ChemStation installation DVD under \Support\ Manuals\Installation.

Agilent 7100 Capillary Electrophoresis System User Manual 75

4 LAN Configuration

Location of the Media Access Control (MAC) Address

The MAC or hardware address of the LAN interfaces is a world-wide, unique identifier. No other network device will have the same hardware address. The MAC address is physically located on the electronic main board in the rear box of the instrument. It is not accessible by the user.

The MAC can be reviewed from a connected instrument by accessing it via a Telnet session as described earlier. Please follow the instructions on Manual Configuration, Using Telnet (“With Telnet” on page 72) and see Figure 11 on page 74 for the location of the MAC address as the second line in the LAN status page.

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System Definition 78 Controlling the Agilent CE Instrument 79 Working with the CE Diagram 84 How to Prepare your Agilent CE Instrument for Analysis 91 Capillary Electrophoresis, CE 92 Capillary Electrophoresis, Plus High Pressure CE+p 136 Capillary Electrochromatography, CEC 148

This chapter provides information on how to set up the CE instrument for an analysis and explains the basic settings.

Agilent Technologies

5 Using the Agilent 7100 Capillary Electrophoresis System

System Definition

After your Agilent 7100 Capillary Electrophoresis System has been properly installed (see “Installing the Instrument” on page 53) you can begin exploring the system. Your Agilent 7100 Capillary Electrophoresis System comprises the CE instrument, the ChemStation and the Lab Advisor/Instrument Utilities software. The CE instrument doesn't have a keyboard itself. It is completely controlled by the ChemStation through the CE and DAD diagram.

For detailed description of data evaluation see the manuals “Understanding Your ChemStation" and “Agilent ChemStation for CE System, Installing and Understanding"

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Controlling the Agilent CE Instrument

Before You Start the Agilent CE Instrument

Use the following as a checklist before you start the Agilent CE instrument.

Make sure that:

• the replenishment bottles are installed and properly tightened,

• the cassette (with a capillary installed) is inserted in the system,

• the cassette door and the tray door are closed, and

• you do not need to put the vials into the tray before you start the

Agilent CE instrument

Starting Up the Agilent CE Instrument

Controlling the Agilent CE Instrument

The power switch of the instrument is located on the left front corner of the instrument.

Press this switch to turn on the line power.

After the instrument is started, the fans and the pump start working. The instrument starts to build-up internal pressure (approximately 950 mbar) first, followed by a vacuum. The pump becomes idle and switches on only occasionally once the pressure and vacuum setpoints are reached.

During initialization the instrument tests different functions and adjustments. The tray as well as all three lift stations are moved.

The UV lamp will switch on during instrument startup based on parameters that can be defined in the DAD control section, see “Diode Array Detector” on page 179. Otherwise, the lamp can be switched on from the DAD diagram of the ChemStation.

The meaning of the LED colors on the front cover are explained in the following table.

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NOTE

Controlling the Agilent CE Instrument

Tabl e 22 Status LED color

Color Meaning

No color Instrument is ready

Green Analysis running, do not use instrument with the exception of manual

operation of the tray. Some fields in DAD and CE diagram are shaded gray.

Yellow Not-ready condition. The run cannot be started yet. When you start, the

LED will come on after a certain time. Click the Agilent CE System status bar in the ChemStation to see what is not ready (for example temperature).

Red Error condition. There was or is currently a problem with the instrument

or communication to the PC, which requires your action or an acknowledgement. To get more information, connect to the instrument with the Lab Advisor or ChemStation software. Check the logbook.

A blinking red LED indicates the instrument is in a resident mode. This means only a core of the firmware is active and the instrument has limited functionality. The Lab Advisor software will be able to connect to the instrument under these circumstances. An instrument restart by switching the power off and on again boots with complete firmware loaded. During a main firmware download (see “Firmware” on page 176), the instrument shows a red blinking LED.

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NOTE

Controlling the Agilent CE Instrument

Starting Your Computer and the Agilent ChemStation Software

After you have switched on your computer and it has booted up, start the Agilent ChemStation by double clicking the Instrument Online icon in the Agilent ChemStation menu.

In order to control the 7100 Capillary Electrophoresis instrument, the ChemStation revision must be B.04.02 or higher.

We recommend you install the Lab Advisor software revision B.01.03 or higher.

If you have problems starting the Agilent ChemStation, check that the software has been properly installed. During startup the ChemStation might ask you to update the connection settings, see Figure 12. You might suppress this message in the future. If you want to update the instrument configuration, select Ye s. Typically, an instrument configuration must be updated for first time use of the software, changes in the IP address or changes in the mode of usage.

Please refer to the ChemStation documentation and online help for details on installation and configuration of the 7100 CE instrument.

Figure 12 Instrument configuration during ChemStation startup

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Controlling the Agilent CE Instrument

Starting the Agilent ChemStation Software the First Time

Starting the Agilent ChemStation software the first time will lead to the following message:

Figure 13 Configuration at first start of ChemStation

Please select Ye s. The following dialog will allow you to configure the instrument. Please provide an IP address or host name in the subsequent dialog. Check the respective radio button and type in either the IP address or the host name of your instrument.

Figure 14 Request of IP address or host name

The auto-configuration process will detect an instrument at the IP address or host name you provide. You can confirm the instruments identity with the serial number.

Please select "Configure..." if you would like to connect to a different instrument.

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Controlling the Agilent CE Instrument

Figure 15 Configuration menu

Please refer to “LAN Configuration” on page 61 for the default IP address or manual configuration of an individual IP address.

After you have double clicked the Instrument Online icon, the software is loaded with the last method used. The software boots in the exact last status.

Do not use the instrument or the graphical user interface during initialization. Wait until the system has reached the ready state.

If there is a vial in tray position 50 (reserved position), the system asks you to remove that vial before you proceed.

Duplicate vials are put in the tray and the respective position is brought to the front. Duplicate vials may occur if vials from the tray are loaded in a lifter and later on another vial is loaded in the same tray position.

If there are no bottles (electrolyte or waste) in the replenishment system or they are not properly tightened, the error LED (red) will come on because no pressure can be built up. Check the logbook for more information.

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Working with the CE Diagram

When you start the Agilent ChemStation, the CE and DAD diagram appears by default as the main screen. The CE diagram displays the Agilent CE instrument showing the current status of the system as a whole. Each icon represents one component in the system.

Common Software Toolbar

The software tool bars in the Agilent ChemStation offer a group of commonly used functions. The software tools bars visible in the Method and Run Control View are shown in Figure 17 on page 86. To change values for a component, you must choose the appropriate icon.

This will bring up the menu. The menu may comprise sets of items; for changing parameters in your method, for control of the instrument, and for help.

The tool bars associated with the Data Analysis View and Report Layout view look similar and are described in the online help and the ChemStation documentation.

For more assistance, click Help in the menu bar.

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Software Toolbar

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Working with the CE Diagram

Figure 16 Components of the common software toolbar of the ChemStation.

1 Work with sequences 8 Change the layout to show two online

plots

2 Work with single vial analysis 9 Change the layout to show two online

plots and the current log book

3 Load method 10 Display the current logbook 17 Display sample information for

4 Store method 11 Display the current sequence logbook 18 Access integration, calibration and

5 Store sequence 12 Open the Preferences dialog 19 Access sequence templates 6 Load sequence 13 Name of current sequence 20 Access methods 7 Change the layout to show the

sampling area and diagram

14 Name of current method

15 Display of elapsed and overall run

time

16 Status display

sequences or single vials

reporting settings

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Working with the CE Diagram

Graphical User Interface Explanation

Components of the Agilent CE instrument in the CE diagram (synonym dashboard) are shown in Figure 17. Each component in the instrument is represented by an icon in the CE diagram or DAD diagram.

Figure 17 The CE and DAD diagram represents each component in the instrument

1 CE control diagram 8 High voltage power supply 15 Method steps and progress 2 DAD control diagram 9 Internal pressure 16 DAD status 3 CE status 10 Replenishment station 17 Lamp 4 Tray / tray temperature 11 Electrolyte bottle / pressure storage 18 Detector wavelengths/spectra 5 Inlet lift / loaded vial 12 Waste bottle / vacuum storage 19 Instrument identifier 6 Outlet lift / loaded vial 13 Current electrical values 20 Overall instrument status 7 Cassette temperature 14 Electrical parameter from method 21 Instrument mode

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Cassette

Detector

Outlet lift

Inlet lift

Replenishment lift

Replenishment bottles

Lid latch

Sample tray

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Working with the CE Diagram

The current analysis mode is displayed in the top line of the CE diagram. The analysis modes that can be selected under instrument configuration are CE, CE+p, CEC or CE-MS.

For configuration without a DAD detector, for example with external detectors, the DAD diagram will not be displayed. For configuration with an additional pump, for example CE-MS, an additional pump diagram will be displayed.

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Figure 18 Corresponding hardware components

5 Using the Agilent 7100 Capillary Electrophoresis System

DAD CE

Working with the CE Diagram

Menus in the CE and DAD Diagram

The CE and DAD diagram offers access to directly control several instrument components, as well as access to method parameters. Right-clicking in the background of each diagram provides access to the context menus (Figure 19).

Figure 19 Menus in the DAD and CE diagram

To trigger actions for an instrument component, select the appropriate icon in the diagram. A context menu including possible actions, such as switching on the lamp, appears. Any instrument action triggered through such a menu does not change the current method.

To change values for a component, select the appropriate icon. A menu with three general items and parameter specific for the component appears.

Method

A click here requests the current method from the instrument and opens the CE Method Setup dialog box for the CE part of the current method or the respective DAD part. To set up a complete method, refer to “How to Use Your

Agilent CE Instrument for Capillary Electrophoresis Separations” on page 92.

If closed with OK the (possibly) modified method is downloaded to the device. If changes have been made an asterisk is added to the method status. The

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method status is represented by a book icon in the top line of the dashboard.

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Working with the CE Diagram

Direct Control of Instrument Components

The actions are performed immediately and the changes are reflected in the CE diagram. Such actions include, for example:

• Switching the lamp on.

• Controlling the sample tray.

• Lowering the lift stations to remove the capillary cassette.

• Releasing the pressure to change the replenishment bottles.

Working with these items does not change the method currently loaded.

Set Error Method

Set Error Method enables you to store the current parameters. These parameters

are applied to the instrument when an error occurs. It ensures that the instrument shuts down in a controlled manner if ChemStation control is discontinued for any reason.

Identify Device

The command Identify Device causes the LED on the front cover to blink alternating in green and yellow for 4 seconds.

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Working with the CE Diagram

System Vialtable

The vialtable is a graphical tool which facilitates the management of vials in the tray. It can be used as an intelligent notepad, which exchanges its information with methods and sequences.

Some features are:

• If a vial is defined in the vialtable this definition (name of vial) will be used

whenever the vial is referenced (method editing, method print).

• Consistency checks can be done in order to verify if there are vial conflicts

between the method and sequence usage of vials.

• Documentation of vial positions and contents.

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How to Prepare your Agilent CE Instrument for Analysis

To prepare the Agilent CE instrument for the analysis you need to do the following tasks:

• Start the Agilent CE instrument.

• Start the computer.

• Start the Agilent ChemStation software/CE user interface.

• Allow the lamp to warm up (approximately 1 hour).

• Prepare vials for sample and buffer.

• Insert a capillary into the alignment interface.

• Insert the capillary into the capillary cassette.

• Insert the capillary cassette into the system.

• Prepare the replenishment system (optional).

After you have completed all these tasks, you can start an Agilent CE method.

A CE method can be created in an online (CE instrument is on and connected) or offline installation of the Agilent ChemStation.

The detector lamp can be switched on automatically and unattended by scheduling this from the detector control menu. Please see the options for the detector under “Diode Array Detector” on page 179.

The detector lamp can be switched on automatically with each instrument start. Select the respective setting from the detector control menu (see “Diode

Array Detector” on page 179).

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Capillary Electrophoresis, CE

How to Use Your Agilent CE Instrument for Capillary Electrophoresis Separations

This chapter introduces method parameters for applications running in CE mode. This mode is the most suitable for applications in the field of capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC) and capillary isoelectric focussing (CIEF). The current CE mode for your instrument can be selected under Instrument < more CE < Instrument

configuration... . The current mode is visible in the second line of the CE control

diagram. For detailed information, see “Working with the CE Diagram” on page 84 and “Graphical User Interface Explanation” on page 86.

Agilent CE Method Parameters

This section describes the parameters of an Agilent CE method for data acquisition and their meanings. Example sets of parameters for a method to analyze the test sample are in “Setting Up a Test Sample Run” on page 252. After you have completely set up the Agilent CE method and supplied the sample information, you can run your method. Choose Run Method from the Run control menu.

Overview

A convenient way to access the parameters of an Agilent CE method is the item Setup Instrument Method in the Instrument menu. This displays the CE Method Setup dialog box. Tabs with parameters for the DAD and CE part of a method can be edited on this screen. The left-hand side shows the most important parameters. The right-hand side offers several pages that can be expanded subsequently for editing.

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Capillary Electrophoresis, CE

Figure 20 CE Method Setup

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Capillary Electrophoresis, CE

Figure 21 DAD Method Setup

Selecting OK transfers the parameters to the instruments and closes the dialog. Choosing Apply only transfers the current parameter to the instrument. Save the edited method, if appropriate.

If you do not save the method to the hard disk, this changed method is executed when choosing Run Method. When you refer to a method in a sequence, the method is always loaded from the disk. So if you did not save your changes using Save Method, the original method from the hard disk is used.

Method parameters are also available if Method/Edit Entire Method... is chosen. This dialog guides you through further parameters associated with a method such as

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Capillary Electrophoresis, CE

• Method Information

• Instrument / Acquisition

• Data Analysis

• Run Time Checklist

The book icon in the software toolbar is a further alternate entry point for method editing. When you click the icon, you can access menu items to set method parameters.

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Capillary Electrophoresis, CE

Vials

You can program a method to use certain vials as the inlet or outlet home vial. During electrophoresis, the electrodes and the capillary are in these vials. Vial numbers 1 to 48 or user vials (User 1 to User 10) can be selected. The selection can be done from the pull-down menu or by typing in the number with the keyboard. Clicking Add Method Vial allows you to enter vial names which will be visible in the vialtable.

Figure 22 Add Method Vial

User vials are numbered from 1 to 10. User vials are placeholders in the method which are resolved by the sequence table at a later point in time. Usage of user vials is helpful for method optimization. Various buffer compositions can be tested with one common method. Creation of methods with variations of Inlet or outlet home vials can be omitted. Usage of user vials is also helpful to change buffers in long sequences to avoid over utilization of the buffer.

Vial numbers below 10 must be typed in with a preceding 0, for example 02 for a vial in tray location number 2, since the vials are numbered with two digits.

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Cassette Temperature

NOTE

The cassette temperature can either be set to not be controlled or to a certain setpoint. This setpoint must be within the instrument’s specified range (see

“Physical Specifications” on page 21).

The lowest applicable temperature is 10 °C below ambient temperature. The highest temperature is 60 °C.

The instrument is ready after the cassette temperature reaches the setpoint and stabilizes. A run can be started

The home values are activated at five points in your analysis:

• When a method is loaded.

• When you activate Run Method or Run Sequence from the RunControl menu.

• After injection is completed.

• After the stoptime has elapsed.

• After the entire analysis (including postconditioning) is completed.

Keep the cassette temperature above the dew point or decrease the ambient humidity.

Using the Agilent 7100 Capillary Electrophoresis System 5

Capillary Electrophoresis, CE

The instrument is specified to be operated at non-condensing conditions which is at humidity below 80% at 31 °C (87.8 °F). Please be aware: if the temperature of the cassette is below the dew point condensation might take place. Subsequently water condensate from the ambient might lead to arcing and damage of the cassette or instrument.

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High Voltage System

The following parameters for the power supply can be controlled using the CE Method Setup screen in the Agilent ChemStation software:

• Enable High Voltage

• Voltage

• Current

• Power

In addition you can set a Lower Alarm Limit for Current.

In order to directly apply HV from the dialog associated with the HV icon (a battery) in the CE dashboard, the active instrument method must have Enable High Voltage check marked.

The maximum stored energy in the CE power-supply is below 200 mJ at 30 kV. Due to this and the fixed current limit of 300 µA the high voltage applied is not defined as hazardous voltage.

Positive voltage settings will configure the electrode at the inlet vial with positive values (positive polarity). Positive polarity is regarded as the standard setting.

You can reverse the polarity to negative by entering negative values for high voltage. This means that a negative voltage is applied at the inlet electrode.

The outlet electrode is always grounded to ensure that the potential at the point of detection is close to ground. Even with negative polarity, the inlet electrode is the point where the voltage is applied and the outlet electrode is close to ground.

Although the outlet electrode is always at the ground potential, you should make sure that the electrode is not short-circuited to ground. The electrode is connected to the power supply to measure the current flowing through the capillary. If the ground electrode is short-circuited to ground, all current flowing through the capillary is signaled as leakage current.

It is good CE practice to ramp up voltage instead of switching voltage suddenly to high values. This is regarded as gentle treatment for samples which might otherwise be affected by the field strength, such as proteins. A linear voltage ramp from 0 to 30 kV can be programed by starting with 0 kV and setting the voltage in the timetable to for example

0.2 minute.

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Voltage, Current and Power

Voltage, current and power are related to each other by the resistance of your capillary/buffer system. You can set values for each of these three parameters. The individual values are treated as limits. The limit that is reached first applies. You can only enter absolute values for current and power.

If you only want to control the voltage and not the current and power, you can set those two to the system limit (typically: 300 µA, 6 W) and the system will permit the maximum value.

Lower Alarm Limit for Current

When using constant voltage mode, the current is normally the indicator of the stability of the system. A rapid current decrease or drop can indicate instability in the system. By setting a lower alarm limit you can define when you want to be warned of such instable conditions during the run.

Instable conditions can be caused by:

• No buffer in the capillary

• Air bubble in the capillary

• Clogged capillary

• Broken capillary

Current values are dependent on the buffer concentration, the type of buffer used, the inner diameter and length of the capillary, and the temperature. When running the Example Method for the Test Sample (see “Setting Up a Test

Sample Run” on page 252) normal current values are below 50 µA.

If you set the lower alarm limit and the it is not exceeded 30 seconds after start of the run, the run is stopped. When the lower alarm limit is reached during the run part of the analysis (when the HV is applied), your current run is stopped. During a sequence the system continues with the next analysis.

Do not use the lower alarm limit or program the time if changing the inlet or outlet vial during the run (for example for fraction collection). Otherwise, the system will stop when changing the vial because the lower alarm limit is reached.

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Posttime and Stoptime

The stoptime defines the time for your separation, also referred to as the run part of the analysis. It does not include replenishment, preconditioning, injection and postconditioning. After the stoptime has elapsed, the system changes from the run state to postconditioning or postrun (if defined) and then to the ready state.

The posttime is normally used for equilibration. It defines the time after the analysis, that is after the stoptime has elapsed and data analysis is completed. During the posttime the instrument is in the postrun state. After both stoptime and posttime have elapsed the instrument completes postconditioning and is then ready for the next analysis.

Replenishment

During multiple analyses, the composition of the buffer can change, for example the pH due to electrolysis. A simple way to refresh the buffer is to access different buffer vials in the tray which might be “user vials” in the method that are assigned to a certain tray position in the sequence table. This is more useful, for example, during method development.

Another way to change the buffer is replenishment. Replenishment can be programmed via the topmost table in the right side of the CE Method Setup screen. It is done either in parallel or serial to the subsequent conditioning operation. The associated timetable can be used to empty, fill or replenish vials to a certain height.

The replenishment system automatically empties and refills the buffer vials in the tray with new buffer from the electrolyte bottle. Therefore, the number of tray positions occupied by home vials is reduced and a maximum of sample vials can be loaded. Typically replenishment is done after every 3 or 4 runs. Depending on the stability of the running buffer, it is sometimes necessary to do it before each run. If you want to use the replenishment system, prepare it as described in “Preparing the Replenishment System if Needed” on page 111.

Using parallel replenishment, you can decrease the total time needed for your analysis, as replenishment and preconditioning are done at the same time. When using this feature, you have to make sure that vials used in conditioning are not replenished at the same time, otherwise you will have a vial conflict.

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Agilent Technologies 7100 User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

User Manual

Agilent 7100 Capillary Electrophoresis System

Contents

Introduction to the Agilent 7100 Capillary Electrophoresis System

Getting Help

Where to Find Information

What Learning Products are Available?

Site Requirements

Power Consideration

Power Cords

Bench Space

Environment

Physical Specifications

Performance Specifications

Anforderungen an den Aufstellungsort

Stromversorgung

Netzkabel

Platzbedarf

Umgebungsbedingungen

Abmessungen und Gewicht

Leistungsspezifikationen

Exigences d’installation

Remarques à propos de l’alimentation

Câbles d’alimentation

Encombrement

Environnement

Caractéristiques physiques

Caractéristiques de performance

Requisiti ambientali

Alimentazione

Cavi di alimentazione

Spazio necessario

Ambiente

Specifiche fisiche

Specifiche delle prestazioni

Requisitos de las instalaciones

Consideraciones sobre la corriente

Cables de alimentación

Espacio en el banco

Entorno

Especificaciones físicas

Especificaciones de rendimiento

設置について

電源について

電源コード

作業台スペース

環境条件

物理的仕様

性能仕様

场地要求

电源要求

工作台位置

物理规格

性能规格

Unpacking the Agilent CE Instrument 54 Installing the Instrument 57

Unpacking the Agilent CE Instrument

Damaged Packaging

Unpacking the CE Instrument

Delivery Checklist

Installing the Instrument

Physical Connection

What You Have to Do First

TCP/IP Parameter Configuration

Configuration Switch

Initialization Mode Selection

Link Configuration Selection

Storing the Settings Permanently with Bootp

Manual Configuration

With Telnet

Agilent Bootp Service

Location of the Media Access Control (MAC) Address

System Definition

Controlling the Agilent CE Instrument

Before You Start the Agilent CE Instrument

Starting Up the Agilent CE Instrument

Starting Your Computer and the Agilent ChemStation Software