Zeta Plus
EXT Series
Featuring Dual Zone ZA Filter Media
The Zeta Plus™ EXT filter media extends depth filtration capacity and extends protection of downstream memb ranes there by
extending filter life and lowering overall filtration costs. Zeta Plus
EXT series is a family of advanced dual zone depth filters designed
to provide optimal clarification of bioprocess, biological and pharmaceutic al fluids. Ze ta P l us EXT series media consist of two distinct
layers, or “zones” of filter media with the upstream zone more open
than the downstream zone. This structure enhances the contaminant ho lding capacity of the filter media, since larger particles are
trapped in the upper zone of the filter media and smaller particles are
trapped in the lower zone, reducing premature plugging and extending service life. The two filter “zones” can be independently
selected and combined to optimize performance.
■
Increased throughput with high turbidimetric efficiency
■
Lower processing costs
■
Smaller filter assemblies
■
Consolidation of multiple filter stages
■
Enhanced protection of downstream membrane filters
Applications
Zeta Plus EXT filters with ZA series media are optimized for clarification of mammalian cell
harvest fluids and for protection of downstream sterilizing grade membrane filters. In
addition, their strong positive charge provides enhanced reduction of negatively charged
contaminants. In solution, cells and cellular debris, endotoxins and contaminating nucleic
acids (DNA, RNA) carry a negative charge. 3M's application of positive zeta potential
to ZA filter media creates a filter matrix that enhances the capture of negatively charged
contaminants for maximum filtration performance. The strong positive charge over a
broad pH range makes these filters ideal for cell broth clarification and for protection of
downstream membrane filters.
Applications
Clarifi cation of mammalian cell culture process fl uids (cell separation)
Protection of downstream processes including membrane
(0.2, 0.1 micron) fi lters and chromatography columns
Clarifi cation of bacteria and yeast cell lysates
Endotoxin and nucleic acid reduction
Clarifi cation of colloidal and haze forming contaminants
™
Superior Performance
Zeta Plus EXT series filters with ZA series media offer unique advantages in contaminant reduction because of their strong electrokinetic properties. In addition to the mechanical exclusion of particles by its depth loading feature, the filter medium adsorbs contaminants
too small for reduction by mechanical straining. Because most particles in suspension are
negatively charged, virtually all contaminants can be reduced with proper media grade
selection.
Zeta Plus™ BC25 & Scale-Up Capsule fi lters are ideal
for feasibility, sizing, small volume, and pilot scale trials.
s
Appli
n
R
s
n
60ZA05A
120ZA08A,
A
n
60ZA05A
A
TFF
n
60ZA05A
A
End
n
90ZA05A
A
G
n
60ZA05A
A
Figure 1: EXT Series with ZA Media Throughput
120 0
600
Throughput (l/m²)
93.7%
92.1%
0
3M
Flux = 5.0 lpm /m²
Competitor A
30
15
Throughput (gal/ft²)
0
Figures 1 through 3 illustrate the superior Zeta Plus™ EXT series performance, tested
using mammalian cell culture fluids, when compared to competitive product. Zeta Plus
EXT series outperform competitive product in each scenario providing both superior
throughput and turbidimetric efficiency. To the end user, this superior performance
translates into lower processing costs, smaller filter assemblies, and enhanced protection
of downstream membrane filters.
Regulatory Support
Zeta Plus EXT series with ZA series media are documented in a Drug Master File (DMF)
on record with the US Food and Drug Administration (FDA ). Contact 3M to request the
fi le number. The product is also supported with a regulatory support fi le and certifi cate of
quality in order to ease validation and regulatory submissions.
Figure 2: EXT Series with ZA Media Throughput
120
60
Throughput (l/m²)
98.6%
99.2%
0
3M
Flux = 2.7 lpm/m²
Competitor B
3
1.5
0
% = Turbidimetric Efficiency
Turbidimetric Efficiency % =
[(Inlet Turbidity - Outlet Turbidity) / Inlet Turbidity] * 100
For example, the efficiency of the first bar
shown on the graph:
Turbidimetric Efficiency = [(37 - 2.34) / 37] * 100 = 93.7%
EXT filters with ZA series media provide up to
2.5 times the throughput with better or equivalent
turbidimetric efficiency.
Figure 3: EXT Series with ZA Media Throughput
2.67 NTU
B
20
10
0
800
400
2.27 NTU
Throughput (l/m²)
0
3M
Competitor
High Turbidity
96 NTU
4.27 NTU
2.88 NTU
Competitor
3M
A
Low Turbidity
17.5 N TU
Even with the varying extremes of inlet turbidity, the
EXT filter media maintains superior throughput and
effluent turbidity when compared to competitive product.
2
Cartridge Construction
Individual cells of Zeta Plus EXT with ZA series media are assembled together with
polypropylene separators under predetermined compression and unitized by three, 316
stainless steel bands.* Each cell is constructed using thermoplastic elastomer molded edge
seals and polypropylene separators for high performance. F ilter cartridges are available
in 8", 1 2", and 1 6 " diameters, with surface area ranging from 0.1 6 m² (1 .8 ft²) to 2. 7 7 m²
(29.8 ft²) per cartridge.
Throughput (gal/ft²)
Capsule Construction
Zeta Plus EXT series is available in autoclavabl e and fully disposable BC25 and Zeta Plus
Encapsulated System capsules. With 25 cm² ( 3 . 9 in²) of fi ltration area, the ready to use
BC fi lter capsule is ideal for feasibility and sizing trials. The Zeta Plus Encapsulated
System includes capsule filters with effective filtration areas at 170 cm² (0.18 ft²), 340 cm²
(0.37 ft²), 1020 cm² (1.10 ft²), 0.23 m² (2.4 ft²), and 1.6 m² (17.1 ft²). They are ideal for both
the scale-up studies as well as large scale production. F or more information on the Zeta
Plus Encapsulated System, please refer to 3M literature 70-0202- 3967 -2.
Pilot Scale and Small Volume
For pilot scale and small volume trials, the Zeta Plus EXT series is available in 8" diameter cartridges or the Zeta Plus Scale-Up Capsule filters. Other units available are 1 - and
2-cell 16" diameter cartridges and the 0.23 m² Zeta Plus Encapsulated filters. Small scale
pilot runs are recommended to confirm grade selection prior to scale-up.
Grade Selection
The Zeta Plus EXT series with ZA filter media are available in six grades. Figure 4
provide a guide to proper grade selection based on nominal retention ratings and can be
used in conjunction with the recommendations in Tab le 1 to determine the appropriate
filter grade for your application. Operating conditions and the fluid being filtered impact
Throughput (gal/ft²)
retention performance. 3M recommends small-scale pilot runs to confirm grade selection
prior to scale-up. 3M's Scientific Applications Support Services ( SASS ) staff can assist in
grade selection as well as assist in on-site evaluatio ns . F i lt er sy st em op timiza ti on c an a ls o
be conducted at 3M Purification's laboratory facilities.
Table 1: Application Recommendation
catio
Mammalian cell broth clarificatio
Bacterial, insect, yeast cell/cell lysate
clarificatio
, membrane protectio
otoxin, nucleic acid reductio
eneral clarificatio
Table 1 is intended as a guide. Grade selection and per formance should be confirmed with small-scale pilot trials.
*Except Z08E05, 8" plug-in style cartridge, which is unitized by a polypropylene post.
ecommended Grade
, 90ZA05A, 90ZA08A,12 0ZA05A,
120ZA10
, 90ZA05A,12 0ZA05A,12 0ZA08
, 90ZA08A,12 0ZA05A,12 0ZA08A, 120ZA10
, 90ZA08A,12 0ZA05A,12 0ZA08A, 120ZA10
, 90ZA05A,12 0ZA05A,12 0ZA08
a
C
n
Surface
ea
05
(
)
0.16
(1.8 ft
)
06
(
)
0.20
(2.1 ft
)
)
0.23
(2.4 ft
)
)
0.23
(2.4 ft
)
)
0
(5.0 ft
)
08
(
)
1
(
1
t
)
2
)
*
a
n
Surface
ea
e
25 c
n
)
0.10 c
f
t
)
Filter
s
M
P
e
BC
-
Plus
s
.
Maxi
O
p
B
)
Zeta Plus
C
)
R
e
)
S
Parameters
BC25
1
(
)
.
Standard Zeta
Plus
s
In situ
stea
ste
(
)
Media
Gasket
Retainer
Gasket
Ring Seal
Stainless
Steel Band
Left: Individual cells of Zeta Plus™ EXT series with ZA
fi lter media are constructed using thermoplastic elastomer molded edge seals and polypropylene separators
for high performance.
Zeta Plus
EXT Series
Featuring Dual Zone ZA Filter Media
™
Internal
Separator
Edge
Seal
Edge
Tab s
Protected
Flow Channels
Cell
Separator
Table 3: Recommended Operating Parameter
aximum
Table 2: Effective Cartridge Surface Are
artridge Confi guratio
Z08 E
Z08 E
8 diameter cartridge plug-in, 5-cell
8 diameter cartridge DOE, 6-cell
Z08 E07 (8 diameter cartridge DOE, 7-cell
Z16 E01 (16 diameter cartridge, 1-cell
Z16 E02 (16 diameter cartridge, 2-cell
Z16 E
16 diameter cartridge, 8-cell
Z16 E12 (16 diameter cartridge, 12-cell)*
Z16E12 (16" diameter car tridge, 12-cell) wet weight (29 kg [65 lb]) may require lifting assistance.
Ar
m
m
m
m
.46 m
.8 m²
9.8 f
.8 m² (29.8 ft
Operating
ressur
mum
perating
Tem
erature
ecommended Pre-use Rins
terilization
Table 3: Effective Capsule Surface Are
Capsule Confi guratio
BC25 Capsul
BCE03 Capsule
Ar
m²(3.9 i
m² (1.10
²
Sanitary Housings
3M provides a wide array of standard and specially designed sanitary filter housings to accommodate Zeta Plus EXT series filter cartridges. All housings are designed with the
pharmaceutical and bioprocessing industries in mind and feature 31 6L polished surfaces
and easy-to-clean components, along with accessories such as CIP spray -balls and the
innovative Zeta Plus Lifting Device. Custom design skid systems incorporating Zeta Plus
and membrane cartridge filter housings, together with process piping, flow control and
complete validation documentation (IQ, OQ), are also available.
2.8 bar (40 psig) maximum
25 Capsules
inlet pressure, 2.4 bar (35
psid) maximum capsule pres
sure drop.
Standard Zeta
Cartridge
2.4 bar (35 psid) maximum
cartridge pressure drop
C25 Capsules 40°C (104°F
82°
Cartridges
(180°F
54 L/m² (1.33 gal/ft²
Autoclave 30 min. at
250°F
(1 cycle)
Autoclave or
Cartridge
rilize 30 minutes @ 126°C
259°F) (3 cycles
Figure 4: Nominal Retention Ratings
60ZA05A
90ZA05A
120ZA05A
90ZA08A
120ZA08A
120ZA10A
4 2 1 0.10.5
Micron
21°C
m
Zeta Plus cartridges can be retrofitted into 3 M’s existing standard Zeta Plus housing offering. T o accommodate both 1- and 2-cell 16" diameter EXT cartridges, 3M has s pecially
designed two sanitary short dome housings that minimize working and hold-up volumes.
An ASME code certified housing is available as well as a light-wei ght band-clamp version. Please call your local 3M Distributor for assistance.
Figure 5: Cartridge Flow vs. Differential Pressure
2
120 ZA08 A
90ZA08A
2
)
2.0
1.5
90ZA05A
1.0
60ZA05A
.5
0
Differential Pressure (bar)
)
08070605040302010
30
25
20
15
10
5
0
Differential Pressure (psid)
02.01.51.00.5
Flux (lpm/m
120Z A10A
Flux (gpm/ft
3
Zeta Plus™ EXT Series Featuring Dual Zone ZA Filter Media Ordering Guide
8" Diameter Cartridges
Catalog Number Configuration Number of Cells Cartridge Construction Gasket Material Grade
Z08 E - EXT 05 - 5 cell
07 - 7 cell
Part Number Example: Z08E05PA60Z A05A. All 8" cartridges are packaged two units per box.
P - Polypropylene
Plug In
A - Stainless Steel Bands
B - Hastelloy® Bands
S - Silicone
60ZA05A 120ZA05A
90ZA05A 120ZA08A
90ZA08A 120ZA10A
12" Diameter Cartridges
Catalog Number Configuration Number of Cells Cartridge Construction Gasket Material Grade
Z12 E - EXT 11 - 11 cell
Part Number Example: Z12E11AA60ZA05A. All 12" cartridges are packaged two units per box.
16" Diameter Cartridges
Catalog
Number
Z16 E - EXT
Part Number Example: Z16E08AA120ZA08A. 1-cell and 2-cell cartridges are each packaged two
units per box. 8-cell cartridges are packaged one unit per box. *Not available in 12 cell.
Configuration
Number
of Cells
01 - 1 c ell
02- 2 cell
08 - 8 cell
12 - 12 cell
Cartridge Construction
A - Stainless Steel Bands
B - Hastelloy® Bands
Gasket
Material
S - Silicon
A - Stainless Steel Bands
B - Hastelloy® Bands
Grade
60ZA05A 120ZA05A
90ZA05A 120ZA08A
90ZA08A 120ZA10A*
S - Silicone
60ZA05A 120ZA05A
90ZA05A 120ZA08A
90ZA08A 120ZA10A
BC Series for Feasibility & Sizing Trials
Catalog Number Grade
BC0025L (Luer)
BC0025S (Sanitary)
BCE03S (Sanitary)
Part Number Example:
BC0025L60ZA05A.
60ZA05A
90ZA05A
90ZA08A
120 ZA0 5A
120 ZA0 8A
120 ZA10A
Important Notice
The information described in this literature is accurate to the best of our knowledge. A variety of factors, however, can affect the performance of the Product(s) in a particular
application, some of which are uniquely within your knowledge and control. INFORMATION IS SUPPLIED UPON THE CONDITION THAT THE PERSONS RECEIVING THE
SAME WILL MAKE THEIR OWN DETERMINATION AS TO ITS SUITABILITY FOR THEIR USE. IN NO EVENT WILL 3M PURIFICATION INC. BE RESPONSIBLE FOR DAMAGES
OF ANY NATURE WHATSOEVER RESULTING FROM THE USE OF OR RELIANCE UPON INFORMATION.
It is your responsibility to determine if additional testing or information is required and if this product is fi t for a particular purpose and suitable in your specifi c application.
3M PURIFICATION INC. MAKES NO REPRESENTATIONS OR WARRANTIES, EITHER EXPRESS OR IMPLIED INCLUDING WITHOUT LIMITATION ANY WARRANTIES OF
MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE OR OF ANY OTHER NATURE HEREUNDER WITH RESPECT TO INFORMATION OR THE PRODUCT TO WHICH
INFORMATION REFERS.
Limitation of Liability
3M Purifi cation Inc. will not be liable for any loss or damage arising from the use of the Product(s), whether direct, indirect, special, incidental, or consequential, regardless of
the legal theory asserted, including warranty, contract, negligence or strict liability. Some states do not allow the exclusion or limitation of incidental or consequential damages,
so the above limitation may not apply to you.
Your Local Distributor:
3M Purifi cation Inc.
400 Research Parkway
Meriden, CT 06450
U.S.A.
(800) 243-6894
(203) 237-5541
Fax (203) 630-4530
www.3Mpurifi cation.com
3M is a trademark of 3M Company.
Zeta Plus is a trademark of 3M Company used under license.
All other trademarks are the property of their respective owners.
© 2012 3M Company. All rights reserved.
70-0201-8862-2
REV 0312
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