21. Appendix H Calibration Record Information ...................................................127
21.1Viewing the Calibration Record (.glp) File ...................................................127
21.2Uploading the Calibration Record (.glp) File................................................127
21.3Understanding the Calibration Record (.glp) File..........................................127
YSI Incorporated YSI 556 MPS
Page iii
1. Safety
1.1 General Information
Read all safety information in this manual carefully before using the YSI
556 Multi-Probe System (MPS). Reagents that are used to calibrate and
check this instrument may be hazardous to your health. Take a moment to
review Appendix D Health and Safety.
WARNING
Warnings are used in this manual when misuse of the instrument could
result in death or serious injury to a person.
CAUTION
Cautions are used in this manual when misuse of the instrument could result
in mild or serious injury to a person and/or damage to equipment.
IMPORTANT SAFETY INSTRUCTIONS!
In essence, the most important safety rule for use of the YSI 556 MPS is to
utilize the instrument ONLY for purposes documented in this manual. This
is particularly true of the YSI 6117 rechargeable battery pack that contains
nickel metal hydride (NiMH) batteries. The user should be certain to read all
of the safety precautions outlined below before using the instrument.
Batteries
This instrument is powered by alkaline or optional nickel-metal hydride
batteries, which the user must remove and dispose of when the batteries no
longer power the instrument. Disposal requirements vary by country and
region, and users are expected to understand and follow the battery disposal
requirements for their specific locale.
The circuit board in this instrument contains a manganese dioxide lithium
"coin cell" battery that must be in place for continuity of power to memory
devices on the board. This battery is not user serviceable or replaceable.
YSI 556 MPS YSI Incorporated
Page 1
SAVE THESE INSTRUCTIONS!
Safety Section 1
When appropriate, an authorized YSI service center will remove this battery
and properly dispose of it, per service and repair policies.
YSI Rechargeable Battery Pack Safety Information
Restrictions on Usage
1. Never dispose of the battery pack in a fire.
2. Do not attempt to disassemble the YSI 6117 battery pack
3. Do not tamper with any of the electronic components or the
batteries within the battery pack. Tampering with either the
electronic circuitry or the batteries will result in the voiding of the
warranty and the compromising of the system performance, but,
more importantly, can cause safety hazards which result from
overcharging such as overheating, venting of gas, and loss of
corrosive electrolyte.
4. Do not charge the battery pack outside the 0–40°C temperature
range.
5. Do not use or store the battery at high temperature, such as in strong
direct sunlight, in cars during hot weather, or directly in front of
heaters.
6. Do not expose the battery pack to water or allow the terminals to
become damp.
7. Avoid striking or dropping the battery pack. If the pack appears to
have sustained damage from these actions or malfunctions after an
impact or drop, the user should not attempt to repair the unit.
Instead, contact YSI Customer Service. Refer to Appendix E
Customer Service.
8. If the battery pack is removed from the YSI 556 MPS, do not store
it in pockets or packaging where metallic objects such as keys can
short between the positive and negative terminals.
Precautions for Users with Small Children.
Keep the battery pack out of reach of babies and small children.
Danger Notifications – Misuse creates a STRONG possibility of death or
serious injury.
YSI 556 MPS YSI Incorporated
Page 2
Safety Section 1
FAILURE TO CAREFULLY OBSERVE THE FOLLOWING
PROCEDURES AND PRECAUTIONS CAN RESULT IN LEAKAGE
OF BATTERY FLUID, HEAT GENERATION, BURSTING, AND
SERIOUS PERSONAL INJURY.
1. Never dispose of the battery pack in a fire or in heat.
2. Never allow the positive and negative terminals of the battery pack to
become shorted or connected with electrically conductive materials.
When the battery pack has been removed from the YSI 556 MPS, store
it in a heavy plastic bag to prevent accidental shorting of the terminals.
3. Never disassemble the battery pack and do not tamper with any of the
electronic components or the batteries within the battery pack. The
battery pack is equipped with a variety of safety features. Accidental
deactivation of any of these safety features can cause a serious hazard to
the user.
4. The NiMH batteries in the battery pack contain a strong alkaline
solution (electrolyte). The alkaline solution is extremely corrosive and
will cause damage to skin or other tissues. If any fluid from the battery
pack comes in contact with a user’s eyes, immediately flush with clean
water and consult a physician immediately. The alkaline solution can
damage eyes and lead to permanent loss of eyesight.
Warning Notifications – Misuse creates a possibility of death or serious
injury
1. Do not allow the battery pack to contact freshwater, seawater, or other
oxidizing reagents that might cause rust and result in heat generation. If
a battery becomes rusted, the gas release vent may no longer operate and
this failure can result in bursting.
2. If electrolyte from the battery pack contacts the skin or clothing,
thoroughly wash the area immediately with clean water. The battery
fluid can irritate the skin.
Caution Notifications – Misuse creates a possibility of mild or serious
injury or damage to the equipment.
1. Do not strike or drop the battery pack. If any impact damage to the
battery pack is suspected, contact YSI Customer Service. Refer to
Appendix E Customer Service.
YSI Incorporated YSI 556 MPS
Page 3
Safety Section 1
2. Store the battery pack out of reach of babies and small children.
3. Store the battery pack between the temperatures of -20 and 30°C.
4. Before using the battery pack, be sure to read the operation manual and
all precautions carefully. Then store this information carefully to use as
a reference when the need arises.
YSI 616 Cigarette Lighter Charger Safety Information
1. This section contains important safety and operating instructions for the
YSI 556 MPS cigarette lighter battery charger (YSI 616; RadioShack
Number 270-1533E). BE SURE TO SAVE THESE INSTRUCTIONS.
2. Before using the YSI 616 cigarette lighter charger, read all instructions
and cautionary markings on battery charger, battery pack, and YSI 556
MPS.
3. Charge the YSI 6117 battery pack with the YSI 616 cigarette lighter
charger ONLY when the YSI 6117 is installed in the YSI 556 MPS.
4. Do not expose charger to rain, moisture, or snow.
5. Use of an attachment not recommended or sold by the battery charger
manufacturer may result in a risk of fire, electric shock, or injury to
persons.
6. To reduce risk of damage to cigarette lighter and cord, pull by cigarette
lighter rather than cord when disconnecting charger.
7. Make sure that the cord is located so that it will not be stepped on,
tripped over, or otherwise subjected to damage or stress.
8. Do not operate charger with damaged cord or cigarette lighter connector
– replace it immediately.
9. Do not operate charger if it has received a sharp blow, been dropped, or
otherwise damaged in any way; contact YSI Customer Service. Refer to
Appendix E Customer Service.
10. Do not disassemble charger other than to change the fuse as instructed.
Replace the part or send it to YSI Product Service if repair is required
(refer to Appendix E Customer Service). Incorrect reassembly may result
in a risk of electric shock or fire.
YSI 556 MPS YSI Incorporated
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Safety Section 1
11. To reduce risk of electric shock, unplug charger before attempting any
maintenance or cleaning. Turning off controls will not reduce this risk.
YSI 556 MPS Water Leakage Safety Information
The YSI 556 MPS has been tested and shown to comply with IP67 criterion,
i.e. submersion in 1 meter of water for 30 minutes with no leakage into
either the battery compartment or the main case. However, if the instrument
is submersed for periods of time in excess of 30 minutes, leakage may occur
with subsequent damage to the batteries, the rechargeable battery pack
circuitry, and/or the electronics in the main case.
If leakage into the battery compartment is observed when using alkaline C
cells, remove batteries, dispose of batteries properly, and dry the battery
compartment completely, ideally using compressed air. If corrosion is
present on the battery terminals, contact YSI Customer Service for
instructions. Refer to Appendix E Customer Service.
If leakage into the battery compartment is observed when using the YSI
rechargeable battery pack, remove the battery assembly and set aside to dry.
Return the battery pack to YSI Product Service for evaluation of possible
damage. Finally dry the battery compartment completely, ideally using
compressed air. If corrosion is present on the battery terminals, contact YSI
Customer Service for instructions. Refer to Appendix E Customer Service.
CAUTION: If water has contacted the rechargeable battery pack, do
not attempt to reuse it until it has been evaluated by YSI Product Service
(refer to Appendix E Customer Service). Failure to follow this precaution
can result in serious injury to the user.
If it is suspected that leakage into the main cavity of the case has occurred,
remove the batteries immediately and return the instrument to YSI Product
Service for damage assessment. Refer to Appendix E Customer Service.
CAUTION: Under no circumstances should the user attempt to open
the main case.
YSI Incorporated YSI 556 MPS
Page 5
2. General Information
2.1 Description
The rugged and reliable YSI 556 MPS (Multi-Probe System) combines the
versatility of an easy-to-use, easy-to-read handheld unit with all the
functionality of a multi-parameter system. Featuring a waterproof, impactresistant case, the YSI 556 MPS simultaneously measures dissolved oxygen,
conductivity, temperature, and optional pH and ORP. A simple cellular
phone style keypad and large display make the instrument easy to use. The
YSI 556 MPS is compatible with YSI EcoWatch
software.
The YSI 556 MPS assists the user in conforming to Good Laboratory
Practice (GLP) standards which help ensure that quality control/quality
assurance methods are followed. Battery life is displayed with a fuel gauge,
and the user can choose standard alkaline batteries or an optional
rechargeable battery pack.
The 1.5 MB memory can store more than 49,000 data sets. Other options
include a flow cell and barometer. The internal barometer can be usercalibrated and displayed along with other data, used in dissolved oxygen
calibrations, and logged to memory for tracking changes in barometric
pressure.
TM
for WindowsTM
Features
• Waterproof -meets IP67 specifications
• Field-replaceable DO electrode module; pH and pH/ORP sensors
TM
• Compatible with EcoWatch
software
• Assists with Good Laboratory Practice Standards (GLP)
• Choice of DO membrane material for different applications
• Easy-to-use, screw-on cap DO membranes
• User-upgradeable software from YSI website
• Three-year warranty on the instrument; one-year on the probe
modules
• Available with 4,10, and 20 m cable lengths
• Stores over 49,000 data sets, time and date stamped
YSI 556 MPS YSI Incorporated
Page 6
for WindowsTM data analysis
Probe Module Section 3
• Auto temperature compensating display contrast
• Optional barometer
• Optional rechargeable battery pack or standard alkaline batteries
2.2 Unpacking the Instrument
1. Remove the instrument from the shipping box. Note that the
probe module and sensors are shipped in a separate box and
will be unpacked later in Section 3.2 Unpacking the Probe Module
NOTE: Do not discard any parts of supplies.
2. Use the packing list to ensure all items are present.
3. Visually inspect all components for damage.
NOTE: If any parts are missing or damaged, contact your
YSI Service Center immediately. Refer to Appendix E
Customer Service or www.ysi.com.
YSI Incorporated YSI 556 MPS
Page 7
Probe Module Section 3
2.3 Features of the YSI 556 Multi-Probe System
On/Off Key
Arrow Keys
Escape Key
Display
Backlight
Key
Enter Key
Alpha/Numeric
Keys – Used to
enter letters and
numbers
Cable Connector
Figure 2.1 Front View of YSI 556 MPS
YSI 556 MPS YSI Incorporated
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Probe Module Section 3
Barometer
Vent Patch
Accessory
Mounting
Holes
Lid
Battery Lid
Screws
Figure 2.2 Back View of YSI 556 MPS
2.4 Batteries
2.4.1 Battery Life
Standard Alkaline Batteries
With the standard battery configuration of 4 alkaline C cells, the YSI 556
MPS will operate continuously for approximately 180 hours. Assuming a
standard usage pattern when sampling of 3 hours of “on time” in a typical
day, the alkaline cells will last approximately 60 days.
Optional Rechargeable Battery Pack
When fully charged, the optional rechargeable battery pack will provide
approximately 50 hours of battery life.
YSI Incorporated YSI 556 MPS
Page 9
Probe Module Section 3
2.4.2 Inserting 4 C Batteries
Figure 2.1 Inserting C Cells
CAUTION: Install batteries properly to avoid damage to the
instrument.
1. Loosen the four screws in the battery lid on the back of the
instrument using any screwdriver.
2. Remove the battery lid.
3. Insert four C batteries between the clips following the polarity
(+ and -) labels on the bottom of the battery compartment.
4. Check gasket for proper placement on the battery lid.
5. Replace the battery lid and tighten the 4 screws securely and
CAUTION: Read all cautions and warning that come with the battery
pack before using the battery pack.
1. Loosen the four screws in the battery lid on the back of the
instrument using any screwdriver.
2. Remove the C battery lid and store for future use. Remove C
batteries, if installed.
3. Install the rechargeable battery pack and lid and tighten the 4
screws securely and evenly.
NOTE: Do not over tighten the screws.
YSI Incorporated YSI 556 MPS
Page 11
Probe Module Section 3
)
2.4.4 Charging the Optional Rechargeable Battery Pack
Charger Adapter cable
(YSI 6119)
Wall Power
Supply (YSI
Figure 2.3 Charging the Battery Pack
6114
CAUTION:Do not use or store the battery pack at extreme
temperatures such as in strong direct sunlight, in cars during hot
weather or close to heaters.
1. Install the rechargeable battery pack into the instrument as
described in Section 2.4.3 Inserting Optional Rechargeable Battery Pack.
2. Attach the charger adapter cable (YSI 6119) to the instrument.
NOTE: Wall power supplies for use in countries outside the US and
Canada can be found in Appendix B Instrument Accessories.
3. Insert the barrel connector of the wall power supply into the
barrel of the adapter cable.
CAUTION: Do not charge the battery pack continuously for more than
48 hours.
CAUTION: Do not drop or expose to water.
CAUTION: Do no charge the battery pack at temperatures below 0°C
or above 40°C.
4. Plug the wall power supply into an AC power outlet for
approximately 2 hours to obtain an 80% to 90% charge for 6
hours to get a full charge.
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Probe Module Section 3
NOTE: The battery pack can be recharged whether the instrument
is on or off.
2.4.5 Storing the Battery Pack
Remove the battery pack from the instrument when the
instrument will not be used for extended periods of time to
prevent over discharge of the battery pack.
Store the battery pack in a heavy plastic bag to prevent
accidental shorting of the terminals. Store between –20 and
30°C.
2.4.6 Optional Cigarette Lighter Charger
CAUTION: Read all warnings and cautions that come with the charger
before using the charger.
CAUTION: Only use cigarette lighter charger when rechargeable
battery pack is inserted into instrument.
CAUTION: Do not mishandle cigarette lighter charger. Do not expose
to moisture.
1. Plug the barrel connector of the cigarette lighter charger into
the mating end of the YSI 6119 Charger Adapter Cable.
2. Attach the MS-19 end of the YSI 6119 Charger Adapter
Cable to the instrument.
3. Make one of the following modifications to the other end of
the charger:
Slide the adapter ring off the plug to use the device with an
American or Japanese vehicle.
.
YSI Incorporated YSI 556 MPS
Page 13
Probe Module Section 3
Figure 2.1 Charger Plug Adapter Use
Leave the adapter ring on the plug and position it so that the slots on
the adapter ring line up with the plug’s spring clips to use the device
on a European vehicle.
Figure 2.2 European Charger Plug Adapter Use
NOTE: If the charger stops working properly, refer to Section 13
Troubleshooting.
2.5 Power On
Press and release the on/off button in the upper left corner of the instrument
keypad to turn the instrument on or off. See Figure2.1 Front View of YSI
556 MPS.
2.6 Setting Display Contrast
The display contrast automatically compensates for temperature changes.
However, under extreme temperature conditions you may wish to optimize
the display by manual adjustment as follows:
YSI 556 MPS YSI Incorporated
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Probe Module Section 3
1. Press and hold down the backlight key in the upper right
corner of the keypad and press the “up” arrow to increase
(darken) the contrast.
2. Press and hold down the backlight key in the upper right
corner of the keypad and press the “down” arrow to decrease
(lighten) the contrast.
2.7 Backlight
Press and release the backlight key in the upper right corner of the keypad
to turn the backlight on or off. See Figure 2.1 Front View of YSI 556 MPS.
NOTE: The backlight turns off automatically after two minutes of non-use.
2.8 General Screen Features
Figure 2.4 Main Screen Menu
Main display
Status Bar
Barometer Reading (optional) –
Updated in real time, not
corrected to sea level
Battery Charge – NiMH label
indicates use of optional
rechargeable battery pack,
pulsing indicates that battery is
charging, flashing indicates
batteries almost exhausted.
1. See Figure 2.10 Keypad Letters & Numbers and press the
appropriate key repeatedly until letter or number desired
appears in display.
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Probe Module Section 3
NOTE: Press the key repeatedly in rapid succession to get to
the desired letter or number. If you pause for more than a
second, the cursor automatically scrolls to the right to prepare
for the next input.
EXAMPLE 1: Press the 6 key once and release to display an
uppercase “M”.
EXAMPLE 2: Press the 6 key four times and release to
display the number “6”.
EXAMPLE 3: Press the 6 key five times and stop to display a
lowercase “m”.
2. Press the left arrow key to go back and reenter a number or
setter that needs to be changed.
Press the Enter key when your entry is complete.
NOTE: The instrument software permits only numeric entries
in many instances, such as when setting the clock or entering
calibration parameters.
2.10 Instrument Reset
The YSI 556 MPS is characterized by sophisticated software that should
provide trouble-free operation. However, as with all high-capability
software packages, it is always possible that the user will encounter
circumstances in which the instrument does not respond to keypad entry. If
this occurs, the instrument function can easily be restored by removing and
then reapplying battery power. Simply remove either your C-cells or
rechargeable battery pack from the battery compartment, wait 30 seconds
and then replace the batteries. See Section 2.4 Batteries for battery
removal/reinstallation instructions.
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Probe Module Section 3
2.11 Menu Flowchart
Run
Report
Sensor
Calibrate
File
Logging Setup
System Setup
Log One Sample
Start Logging
Temperature
Conductivity
Dissolved Oxygen
pH
ORP
Condutivity
DO
pH
ORP
Interval
Use Site List
Store Barometer
Store Site Number
Edit Site List
Temperature
Specific Conductance
Conductivity
Resistivity
TDS
Salinity
DO % Saturation
DO mg/L
pH
pH mV
ORP
Directory
Upload to PC
Plot file
View file
File memory
Delete all files
Version
Language
Data & time
Data filter
Shut off time
Comma Radix
ID
Circuit board SN
GLP file name
TDS Constant
Barometer Units
Calibrate barometer
YSI 556 MPS YSI Incorporated
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3. Probe Module
3.1 Introduction
The YSI 5563 Probe module is used for measuring dissolved oxygen,
temperature, conductivity, and optional pH and ORP. The probe module is
rugged, with the sensors enclosed in a heavy duty probe sensor guard with
attached sinking weight. A 4, 10 or 20 meter cable is directly connected to
the probe module body making it waterproof. An MS-19 connector at the
end of the cable makes the YSI 5563 fully compatible with the YSI 556
Multi-Probe System.
3.2 Unpacking the Probe Module
1. Remove the YSI 5563 Probe Module from the shipping
boxes.
NOTE: Do not discard any parts or supplies.
2. Use the packing list to ensure all items are present.
3. Visually inspect all components for damage.
NOTE: If any parts are missing or damaged, contact a YSI
representative immediately. Refer to: Appendix E Customer Service o visit www.ysi.com.
YSI 556 MPS YSI Incorporated
Page 19
Probe Module Section 3
3.3 Features of the YSI 5563 Probe Module
Cable
Dissolved Oxygen
(DO) Probe
Transport/
Calibration Cup
Figure 3.1 Probe Module
3.4 Preparing the Probe Module
To prepare the probe module for calibration and operation, you need to
install the sensors into the connectors on the probe module bulkhead. In
addition to sensor installation, you need to install a new DO membrane cap.
Strain Relief
Metal Probe
Connector Nut
pH/ORP Probe
Conductivity/
Temperature
Probe
Probe Sensor Guard
3.4.1 Sensor Installation
Whenever you install, remove or replace a sensor, it is extremely important
that the entire probe module and all sensors be thoroughly dried prior to the
removal of a sensor or a sensor port plug. This will prevent water from
entering the port. Once you remove a sensor or plug, examine the connector
inside the probe module sensor port. If any moisture is present, use
compressed air to completely dry the connector. If the connector is
corroded, return the probe module to your YSI Distributor or directly to YSI
Customer Service. Refer to Appendix E Customer Service.
YSI 556 MPS YSI Incorporated
Page 20
Probe Module Section 3
port
port
Conductivity/Temperature and pH, pH/ORP Sensor Installation
1. Unscrew and remove the probe sensor guard.
2. Using the sensor installation tool supplied in the YSI 5511
maintenance kit, unscrew and remove the sensor port plugs.
Figure 3.2 Port Plug Removal
3. Locate the port with the connector that corresponds to the
sensor that is to be installed.
pH or pH/ORP port
Dissolved Oxygen
Figure 3.3 Sensor Port Identification
4. Apply a thin coat of o-ring lubricant (supplied in the YSI
5511 maintenance kit) to the o-rings on the connector side of
the sensor (see Figure 3.4 O-ring Lubrication).
YSI Incorporated YSI 556 MPS
Conductivity/Temperature
Page 21
Probe Module Section 3
O-Rings
Sensor nut
Figure 3.4 O-ring Lubrication
CAUTION: Make sure that there are NO contaminants between the o-ring
and the sensor. Contaminants that are present under the o-ring may cause
the o-ring to leak.
5. Be sure the probe module sensor port is free of moisture and
then insert the sensor into the correct port. Gently rotate the
sensor until the two connectors align.
6. With the connectors aligned, screw down the sensor nut using
the sensor installation tool.
Figure 3.5 Sensor Installation
CAUTION: Do not cross thread the sensor nut. Tighten the nut until it is
flush with the face of the probe module bulkhead. Do not over tighten.
Figure 3.6 Bulkhead Seating
7. Repeat steps 3-6 for any other sensors.
YSI 556 MPS YSI Incorporated
Page 22
Probe Module Section 3
8. Replace the probe sensor guard.
Dissolved Oxygen Sensor Installation
The YSI 5563 comes with the DO sensor already installed. Refer to Section
11.1.2 DO Sensor Replacement for instructions on installing the YSI 559
Replaceable DO Module Kit.
3.4.2 Membrane Cap Selection
The YSI 5563 is shipped with a YSI 5909 kit that contains membrane caps
made with 2 mil polyethylene (PE), a material which should be ideal for
most field applications of the 556. However, YSI also offers membrane caps
made with two other materials (1 mil polyethylene and 1 mil Teflon) which
some users may also prefer. All membranes available for the 556/5563
system provide comparable accuracy if used properly. The difference
between the two thicknesses of PE is found in the trade-off of flow
dependence and response time as described below. Teflon is offered because
some users may prefer to continue using the traditional membrane material
used by YSI. To avoid confusion, the membrane caps are color coded as
described below and can be ordered in kits as noted:
1 mil Teflon – Black Caps (Kit = YSI 5906)
1 mil Polyethylene (PE) – Yellow Caps (Kit = YSI 5908)
2 mil Polyethylene (PE) – Blue Caps (Kit = YSI 5909)
The 1 mil Teflon caps will offer traditional, reliable performance for most
dissolved oxygen applications. The 1 mil PE caps will provide a
significantly faster dissolved oxygen response (as long as your 556 Data
Filter is set correctly as described below in Sections 10.2 and 10.3.1) while
also giving readings which are significantly less flow dependent than the 1
mil Teflon caps. Finally, 2 mil PE caps will show a large reduction in flow
dependence over 1 mil Teflon while not significantly increasing the
response time. Generally, one of the PE caps is likely to provide better
performance for your application.
IMPORTANT: No matter which type of membrane cap you select, you
will have to confirm your selection in the 556 software from the Sensor
menu as described in Section 4 Sensors.
YSI Incorporated YSI 556 MPS
Page 23
Probe Module Section 3
3.4.3 Membrane Cap Installation
NOTE: The YSI 5563 DO sensor (already installed in the probe module)
was shipped dry. A shipping membrane was installed to protect the
electrode. A new membrane cap must be installed before the first use.
1. Unscrew and remove the probe sensor guard.
2. Unscrew, remove, and discard the old membrane cap.
3. Thoroughly rinse the sensor tip with distilled water.
4. Prepare the electrolyte according to the directions on the
electrolyte solution bottle.
5. Hold the new membrane cap and fill it at least ½ full with the
electrolyte solution.
6. Screw the membrane cap onto the sensor moderately tight. A
small amount of electrolyte should overflow.
Caution: Do not touch the membrane surface.
7. Screw the probe sensor guard on moderately tight.
3.5 Transport/Calibration Cup
The YSI 5563 Probe module has been supplied with a convenient
transport/calibration cup. This cup is an ideal container for calibration of the
different sensors, minimizing the amount of solution needed. Refer to
Section 6 Calibrate.
YSI 556 MPS YSI Incorporated
Page 24
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