UVP MultiDoc-It Imaging System User Manual

MultiDoc-It™ Imaging System

Installation and User Instructions

UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]

Web Site: www.uvp.com

81-0214-01 Rev K

MultiDoc-It Imaging System 2

Overhead white light

Benchtop UV

Introduction

The MultiDoc-It Imaging System is a high resolution imager capable of capturing and documenting
fluorescent gel images. The system combines:

1. MultiDoc-It Darkroom

2. DigiCam digital color camera

3. Ethidium Bromide (EtBr) Filter

4. Doc-It

5. UV transilluminator (transilluminator instructions are provided in a separate manual)

NOTE: MultiDoc-It TLC Systems (for Thin Layer Chromatography) do not include a transilluminator.

®

LS Acquisition Software

Small footprint

darkroom

UV-blocking Gel

Viewer Window

Wide access door

DigiCam camera

with diopter

Three position filter tray;

EtBr filter included

Side access doors

(optional)

Transilluminator

Darkroom

The MultiDoc-It darkroom provides a dark environment for gel UV illumination and protects the DigiCam
camera. Some MultiDoc-It models include side access doors for moving or cutting gels without
opening the front door.

UV Filter

The included emission filter, an orange-colored UV blocking bandpass filter, is used to absorb UV and
IR radiation from the transilluminator and to enhance the orange/pink bands generated by Ethidium
Bromide stained gels. The filter can be removed when imaging non-fluorescent media (protein gels,
colony plates, etc.) in order to produce brighter images.

Camera and Diopter

The DigiCam digital color camera includes a built-in optical zoom lens. A close-up diopter is connected
to the lens at the UVP factory.

MultiDoc-It Imaging System 3

Doc-ItLS Acquisition Software

Doc-ItLS Software

The Doc-ItLS software loads onto the user’s existing computer. The software controls the camera
acquisition functions as well as enhancement and reporting features. For information on operation of the
software, refer to the Doc-ItLS software help files.

UV Transilluminator

A UV transilluminator may be shipped with the system. Refer to the Transilluminator manual for
operating instructions.

Operating System Requirements

• Windows 7, Vista or XP SP2 or later

• Internet Explorer 6.0 or higher [To locate the version of Internet Explorer, open Internet

Explorer and click on He lp > About Internet Explorer]

• Intel Pentium Processor or equivalent, 1.6 GHz or higher

• 1 GB of RAM or greater

• 200 MB of available hard disk space for the program, more for data

• CD-ROM drive

• One Universal Serial Bus (USB) for connecting the camera

• Color monitor, supporting at least 1024 x 768 resolution and 16-bit or better colors; 24-bit or

32-bit color is strongly recommended

NOTE: For 21 CFR Part 11 support functionality, the computer partition must be formatted with NTFS.

Refer to the Doc-ItLS software manual for instructions.