UVP Life Science User Manual
Life Science Software
Installation and User Guide
• Doc-It®LS Image Acquisition Software
• Doc-It®LS Image Acquisition and Analysis Software
• VisionWorks®LS Image Acquisition Software
• VisionWorks®LS Image Acquisition and Analysis Software
UVP, LLC Ultra-Violet Products Ltd.
2066 W. 11th Street Unit 1, Trinity Hall Farm Est., Nuffield Rd.
Upland, CA 91786 USA Cambridge CB4 1TG UK
(800) 452-6788 | (909) 946-3197 +44(0)1223-420022
Fax: (909) 946-3597 Fax: +44(0)1223-420561
Email: info@uvp.com Email: uvp@uvp.co.uk
Web site: UVP.com
81-0254-01 Rev L
Table of Contents
Welcome ....................................................................................................................................................... 1
Welcome to LS Software Guide .................................................................................................................... 1
What's New in Version 8 ........................................................................................................................... 1
Getting Started .......................................................................................................................................... 1
Capturing Images ................................................................................................................................. 2
Performing Analysis Functions on Images ....................................................................................... 2
Minimum System Requirements ................................................................................................................... 3
Operating System Requirements .............................................................................................................. 3
Registering the Software ............................................................................................................................... 4
Overview .................................................................................................................................................... 4
Types of Registration ................................................................................................................................. 4
On the Fly Activation .............................................................................................................................. 4
Already Have an Activation ID ............................................................................................................... 5
Offline Activation .................................................................................................................................... 5
Single-User License ...................................................................................................................................... 7
Network-User License ................................................................................................................................... 8
Additional Network Users ........................................................................................................................ 12
User Administration ..................................................................................................................................... 14
About Secure User Accounts .................................................................................................................. 14
Enable Secure User Accounts .......................................................................................................... 14
Definitions of the User Administration Columns ........................................................................... 15
Users Rights ............................................................................................................................................ 16
Configure User Accounts ............................................................................................................................ 17
User Names and Passwords ................................................................................................................... 17
Add a New User ...................................................................................................................................... 18
Edit a User ............................................................................................................................................... 18
Change a Password or Other Settings .................................................................................................... 19
Deactivate/Reactivate a User .................................................................................................................. 19
View the Login History of a User ............................................................................................................. 20
T
echnical Support ....................................................................................................................................... 21
License Agreement ..................................................................................................................................... 22
End User License Agreement ................................................................................................................. 22
VisionWorks®LS and Doc-It®LS Software ............................................................................................. 22
License ................................................................................................................................................ 22
Restrictions ........................................................................................................................................ 22
Termination ........................................................................................................................................ 22
Limited Warranty and Disclaimer ..................................................................................................... 23
Limitation of Liability ......................................................................................................................... 23
Governing Law and Severability ...................................................................................................... 23
Navigate the Software ................................................................................................................................. 24
Navigating the Software .............................................................................................................................. 24
Main Window ........................................................................................................................................... 24
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Table of Contents
Action Tabs .............................................................................................................................................. 24
Image Windows ........................................................................................................................................... 26
Organize Image Windows ....................................................................................................................... 26
Information Provided by the Image Window ............................................................................................ 26
Show the Image in Actual Size ................................................................................................................ 27
Fit the Image to the Window .................................................................................................................... 27
Context Menu Commands ....................................................................................................................... 27
Status Bar ................................................................................................................................................ 27
Obtain Image Information ............................................................................................................................ 28
Overview .................................................................................................................................................. 28
Display Image Information ....................................................................................................................... 28
Enter Notes .............................................................................................................................................. 28
Calibrate Image Scale ............................................................................................................................. 28
Menu Buttons .......................................................................................................................................... 30
Menus and Action Tabs Overview .............................................................................................................. 30
Main Menu Buttons .................................................................................................................................. 30
Action Menu Tabs .................................................................................................................................... 30
Acquisition Action Tab and Menu Buttons ..................................................................................... 30
Image Action Tab and Menu Buttons .................................................................................................. 30
1D Analysis Action Tab and Menu Buttons ......................................................................................... 30
Area Density Action Tab and Menu Buttons ........................................................................................ 31
Colony Counting Action Tab and Menu Buttons .................................................................................. 31
File Menu Button .................................................................................................................................. 32
File Menu Overview .................................................................................................................................... 32
Open and Save Images .............................................................................................................................. 33
Open Images ........................................................................................................................................... 33
Open a Previously Saved Image ...................................................................................................... 33
Open a Demo Image .......................................................................................................................... 33
Save Images ............................................................................................................................................ 33
Save Using a Different File Folder, Name or Type ......................................................................... 34
I
mage File Types ..................................................................................................................................... 34
Print ............................................................................................................................................................. 35
FTP Transfer ............................................................................................................................................... 36
Edit Menu Button ................................................................................................................................. 38
Edit Menu Button Overview......................................................................................................................... 38
Copy ............................................................................................................................................................ 39
Copy an Entire Image .............................................................................................................................. 39
Copy a Selected Region Within an Image ............................................................................................... 39
Paste ........................................................................................................................................................... 40
Paste an Image ....................................................................................................................................... 40
Paste Special ........................................................................................................................................... 40
Undo and Redo ........................................................................................................................................... 42
Region of Interest (ROI) .............................................................................................................................. 43
About the Selection Tools........................................................................................................................ 43
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LS Software User Guide
Select a Region ....................................................................................................................................... 44
Adjust a Region ....................................................................................................................................... 44
Cancel a Region ...................................................................................................................................... 44
Image Enhancement Filters ........................................................................................................................ 45
Blur .............................................................................................................................................................. 46
Sharpen ....................................................................................................................................................... 47
Emboss ....................................................................................................................................................... 48
Remove Noise ............................................................................................................................................. 49
Adjust the Image ......................................................................................................................................... 50
Rotate Function ........................................................................................................................................... 51
Rotate an Image ...................................................................................................................................... 51
Rotate an Image by an Exact Number of Degrees ................................................................................. 51
Align the Image ........................................................................................................................................... 53
Flip Image.................................................................................................................................................... 54
Flip Horizontally ....................................................................................................................................... 54
Flip Vertically ........................................................................................................................................... 54
Resize Image .............................................................................................................................................. 56
Reduce Image to Monochrome ................................................................................................................... 57
Change Image Depth .................................................................................................................................. 58
Advanced Menu Button........................................................................................................................ 59
Advanced Menu Overview .......................................................................................................................... 59
Log Viewer .................................................................................................................................................. 60
Configure User Accounts ............................................................................................................................ 61
User Names and Passwords ................................................................................................................... 61
Add a New User ...................................................................................................................................... 62
Edit a User ............................................................................................................................................... 62
Change a Password or Other Settings .................................................................................................... 63
Deactivate/Reactivate a User .................................................................................................................. 63
View the Login History of a User ............................................................................................................. 64
D
isplay Loaded Modules ............................................................................................................................. 65
Configure Application .................................................................................................................................. 66
Rulers .......................................................................................................................................................... 67
Show or Hide the Rulers .......................................................................................................................... 67
Using Macros .............................................................................................................................................. 68
Overview .................................................................................................................................................. 68
Macros Navigation ................................................................................................................................... 68
Record Macros ........................................................................................................................................ 68
Edit Macros .............................................................................................................................................. 69
Play a User-Defined Macro ..................................................................................................................... 69
Help Menu Button ................................................................................................................................ 70
Help Menu Overview ................................................................................................................................... 70
Display Help Topics .................................................................................................................................... 71
Application Information ................................................................................................................................ 72
Acquire Images ........................................................................................................................................... 73
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Table of Contents
Acquisition Action Tab Overview ................................................................................................................ 73
Image Acquisition ........................................................................................................................................ 75
Capture an Image .................................................................................................................................... 75
Using the iBox Explorer Imaging Microscope ............................................................................................. 77
Emission Filters ....................................................................................................................................... 77
Magnification ........................................................................................................................................... 78
Fine Focus ............................................................................................................................................... 79
Bookmarks ............................................................................................................................................... 79
New ...................................................................................................................................................... 79
Update ................................................................................................................................................. 79
Delete ................................................................................................................................................... 80
Clear..................................................................................................................................................... 80
Lighting/Darkroom/Lens .......................................................................................................................... 81
BioLite MultiSpectral Light Source .............................................................................................................. 81
Using the BioLite Functions ..................................................................................................................... 81
Darkroom Control ........................................................................................................................................ 83
Hardware Settings Selection ................................................................................................................... 83
Tray Height .............................................................................................................................................. 83
Lens Control ................................................................................................................................................ 85
Lens Control ............................................................................................................................................ 85
Camera .................................................................................................................................................... 86
Camera Functions ....................................................................................................................................... 86
Integration ................................................................................................................................................ 86
Exposure Time ........................................................................................................................................ 87
Gain ......................................................................................................................................................... 87
Post Processing ....................................................................................................................................... 88
Binning ..................................................................................................................................................... 88
Saturation Preview .................................................................................................................................. 88
Preferences ............................................................................................................................................. 88
C
CD Temperature ................................................................................................................................... 89
Camera Integration ..................................................................................................................................... 90
On Chip Integration ................................................................................................................................. 90
Sequential Integration .............................................................................................................................. 90
Dynamic Integration ................................................................................................................................. 92
Binning Modes ............................................................................................................................................ 93
Perform 1D Analysis ................................................................................................................................... 94
1D Analysis Action Tab Overview ............................................................................................................... 94
Lanes and Bands ..................................................................................................................................... 97
Finding Lanes and Bands ........................................................................................................................... 97
Define Region of Interest (ROI) ............................................................................................................... 97
Perform Automatic Finding of Lanes and Bands ..................................................................................... 97
Adjust Lanes and Bands Search Parameters ......................................................................................... 98
Lane and Band Information ....................................................................................................................... 100
Lane and Band Properties ..................................................................................................................... 100
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LS Software User Guide
View and Use Lane Properties ....................................................................................................... 100
View and Use Band Properties ....................................................................................................... 101
Information Found in the 1D Analysis Image Window .......................................................................... 101
Add Lanes and Bands ............................................................................................................................... 103
Add Lanes Method No. 1: From the Find Lanes and Bands Window ................................................... 103
Add Bands Method No. 1: From the Find Lanes and Bands Dialog Window ....................................... 103
Add Lanes Method No. 2: From the Image Window ............................................................................. 103
Add Bands Method No. 2: From the Image Window ............................................................................. 104
Delete Lanes and Bands ........................................................................................................................... 106
Delete Lanes ......................................................................................................................................... 106
Delete Bands ......................................................................................................................................... 106
Edit Lanes and Bands ............................................................................................................................... 107
Move Lanes and Bands ......................................................................................................................... 107
Resize Band Extents ............................................................................................................................. 107
Place Bands Exactly .............................................................................................................................. 108
Lane Profile ........................................................................................................................................... 110
Lane Profile Overview ............................................................................................................................... 110
Molecular Weight ............................................................................................................................... 112
Perform Molecular Weight Calibrations .................................................................................................... 112
Overview ................................................................................................................................................ 112
Apply a Molecular Weight Standard to a Lane.......................................................................................... 113
Calibrate a Lane .................................................................................................................................... 113
Stretch Factor ........................................................................................................................................ 114
Manual Placement of Weights ............................................................................................................... 115
Exact Placement of Bands .................................................................................................................... 115
Changing Molecular Weight Settings ........................................................................................................ 116
Add Molecular Weight Standard to the Library...................................................................................... 116
Edit Molecular Weight Standard in the Library ...................................................................................... 117
Remove Molecular Weight Standard in the Library ............................................................................... 117
Copy Molecular Weight Standard in the Library .................................................................................... 117
Background Correction ............................................................................................................................. 118
B
ackground Correction Options ............................................................................................................ 118
No Background Correction ............................................................................................................. 118
Straight Line ..................................................................................................................................... 118
Joined Valleys .................................................................................................................................. 119
Rolling Disc ...................................................................................................................................... 120
Area Between Lanes ........................................................................................................................ 120
Concentration ..................................................................................................................................... 122
Lane Profile Graph .................................................................................................................................... 122
Use the Lane Profile Graph ................................................................................................................... 122
Display Lane(s) ...................................................................................................................................... 122
Axis Options .......................................................................................................................................... 123
Change Axis Units ........................................................................................................................... 123
Display Options ..................................................................................................................................... 123
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Display Options for Details ............................................................................................................. 124
Selecting Background Color .......................................................................................................... 124
Change the Color of the Graph's Axes .......................................................................................... 124
Background Correction Options .................................................................................................... 124
Move Bands Using the Lane Profile Graph ........................................................................................... 124
Resize Bands Using the Lane Profile Graph ......................................................................................... 125
Concentration Calibration .......................................................................................................................... 126
Change Unit Type .................................................................................................................................. 127
Select Unit Type .................................................................................................................................... 127
Add, Edit or Delete a Unit Type ............................................................................................................. 127
Add Unit Type ................................................................................................................................... 127
Edit Unit Type ................................................................................................................................... 128
Delete Unit Type ............................................................................................................................... 128
Select Data Points ................................................................................................................................. 128
Edit Data Points ............................................................................................................................... 129
Delete Data Points ........................................................................................................................... 129
Select Curve Type ................................................................................................................................. 129
Remove Concentration Calibration ....................................................................................................... 130
Retardation factor (Rf) Lines ..................................................................................................................... 131
Automatic Rf Line Determination ........................................................................................................... 131
Adjust an Automatic Rf Line .................................................................................................................. 131
Add Rf Lines Manually ........................................................................................................................... 132
Move Rf Lines ........................................................................................................................................ 132
Delete Rf Lines ...................................................................................................................................... 132
Delete One Rf Line ........................................................................................................................... 132
Remove All Rf Lines ........................................................................................................................ 133
Dendrogram Analysis ............................................................................................................................ 134
Performing Dendrogram Analysis ............................................................................................................. 134
Overview ................................................................................................................................................ 134
Generate Dendrogram Graphs .............................................................................................................. 134
G
enerate Multi-Image Dendrogram Graphs .................................................................................. 135
Modify Dendrogram Graphs .................................................................................................................. 135
Dendrogram Standards ......................................................................................................................... 135
Matching bands ................................................................................................................................ 135
Similarity and distance between lanes .......................................................................................... 136
Create Clusters ...................................................................................................................................... 136
Linkage Rules ........................................................................................................................................... 137
1D Analysis Settings ................................................................................................................................. 139
Results ................................................................................................................................................... 141
Viewing and Printing 1D Gel Analysis ....................................................................................................... 141
Overview ................................................................................................................................................ 141
Results Data Explorer ............................................................................................................................... 142
Filtering Data ......................................................................................................................................... 142
Printing Data Explorer Tabular Reports .................................................................................................... 143
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LS Software User Guide
Print Data Explorer Reports................................................................................................................... 143
Page Setup ........................................................................................................................................ 143
Print Reports .................................................................................................................................... 143
Export Data Explorer Reports ............................................................................................................... 143
Report Types ......................................................................................................................................... 144
Fixed Image and Analysis Reports ........................................................................................................ 144
Fixed Standard Reports .................................................................................................................. 144
Clear ...................................................................................................................................................... 146
Clear Lane and Band Information ............................................................................................................. 146
Perform Area Density Analysis ................................................................................................................. 147
Area Density Action Tab Overview ........................................................................................................... 147
Perform Area Density ................................................................................................................................ 149
Overview ................................................................................................................................................ 149
Perform Area Density Analysis .............................................................................................................. 149
Define the Region ............................................................................................................................ 149
Use Magic Wand to Define ROI ............................................................................................................ 150
Select Multiple Bands and Regions in the Image .................................................................................. 151
Modify Regions in the Image ................................................................................................................. 151
Area Density Settings ................................................................................................................................ 153
Define Area Density Background .............................................................................................................. 154
Define Background ................................................................................................................................ 154
Delete Background Region.................................................................................................................... 155
Modify an Existing Background Region ................................................................................................. 155
Estimate Region Volume ........................................................................................................................... 156
Calibration Curves ..................................................................................................................................... 158
About Intensity Calibration Curves ........................................................................................................ 158
Optical Density ...................................................................................................................................... 158
Grey Levels ........................................................................................................................................... 158
Types of Calibration Curves .................................................................................................................. 159
Apply Pre-Defined Intensity Calibration Curve ...................................................................................... 159
A
dd New Intensity Calibration Curve ..................................................................................................... 159
Set Amount Calibration Curve ............................................................................................................... 160
Change Calibration Curve Graph .......................................................................................................... 161
Reporting and Printing Area Density Results ............................................................................................ 163
Area Density Results and Data ............................................................................................................. 163
Save the Results ................................................................................................................................... 164
Print the Results .................................................................................................................................... 164
Export the Results ................................................................................................................................. 164
Perform Colony Counting .......................................................................................................................... 165
Colony Count Action Tab Overview .......................................................................................................... 165
Colony Counting Settings .......................................................................................................................... 167
Label Type ............................................................................................................................................. 167
Colony Marking ...................................................................................................................................... 168
Label Color ............................................................................................................................................ 168
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Table of Contents
Circle Radius ......................................................................................................................................... 168
Count Colonies ...................................................................................................................................... 169
Counting Colonies - Options ..................................................................................................................... 169
Automatic Counting ................................................................................................................................... 170
Click and Add Counting ............................................................................................................................. 172
Set-Up Colony Counting Templates ......................................................................................................... 174
Counting Using a Predefined Template .................................................................................................... 178
Edit Colonies ...................................................................................................................................... 179
Add Colonies ............................................................................................................................................. 179
Delete Colonies ......................................................................................................................................... 180
Split Colonies ............................................................................................................................................ 181
Manual Split Colonies ............................................................................................................................ 181
Auto Split Colonies ................................................................................................................................ 182
Merging Colonies ...................................................................................................................................... 183
Identify by Color Counting ................................................................................................................. 184
Identify by Color Counting ......................................................................................................................... 184
Identify by Color Counting Step 1: Select Classes ................................................................................... 186
Define the Counting Region .................................................................................................................. 186
Select Classes ....................................................................................................................................... 186
Identify by Color Counting Step 2: Finish .................................................................................................. 189
Spiral Counting .......................................................................................................................................... 191
Perform Spiral Count ............................................................................................................................. 191
Adjust Grid Size and Volume................................................................................................................. 192
View, Save and Print Spiral Plate Results ............................................................................................. 193
User Tips ............................................................................................................................................... 194
Zone Analysis ............................................................................................................................................ 195
Colony Counting Results ....................................................................................................................... 196
Colony Counting Reports .......................................................................................................................... 196
Export and Print Colony Results ............................................................................................................... 197
E
xporting to Excel .................................................................................................................................. 197
Printing Colony Results ......................................................................................................................... 197
Reporting Colony Class Information ......................................................................................................... 198
Reporting General Colony Information ..................................................................................................... 200
Reporting Colony Statistical Information ................................................................................................... 201
Reporting Colony Distribution Information ................................................................................................ 203
Modify Images ........................................................................................................................................... 204
Image Action Tab Overview ...................................................................................................................... 204
Display Control ...................................................................................................................................... 205
Image Corrections ..................................................................................................................................... 205
Corrections ............................................................................................................................................ 205
Change Brightness, Contrast, Gamma Or Invert .................................................................................. 206
Change Brightness .......................................................................................................................... 206
Change Contrast .............................................................................................................................. 206
Change Gamma ................................................................................................................................ 207
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LS Software User Guide
Invert Image ...................................................................................................................................... 207
Histogram Controls ................................................................................................................................... 208
Apply a Histogram ................................................................................................................................. 208
Pseudocolor .............................................................................................................................................. 210
Pseudocolor Spectrums ........................................................................................................................ 210
Apply or Remove a Pseudocolor ........................................................................................................... 211
3D Plots ..................................................................................................................................................... 212
Using the 3D Plot Function .................................................................................................................... 212
Viewpoint Tab ........................................................................................................................................ 213
Rotation Controls ............................................................................................................................. 213
Zoom Controls ................................................................................................................................. 213
Output Tab ............................................................................................................................................. 213
Annotations ............................................................................................................................................ 215
Annotations ............................................................................................................................................... 215
Overview ................................................................................................................................................ 215
Annotation Navigation ........................................................................................................................... 215
Types Of Annotation .............................................................................................................................. 215
Annotation Tools .................................................................................................................................... 216
Create Annotations ................................................................................................................................... 217
Create a Text Annotation....................................................................................................................... 217
Create Line Annotations ............................................................................................................................ 219
Create Shape and Highlight Annotations .................................................................................................. 220
Create a Rectangle, Ellipse, Highlighter Annotation ............................................................................. 220
View or Hide Annotations .......................................................................................................................... 221
Modify Annotations .................................................................................................................................... 222
Select Annotations ................................................................................................................................. 222
Edit Text Annotation .............................................................................................................................. 222
Move and Resize Annotations ............................................................................................................... 222
Move an Annotation ......................................................................................................................... 223
Change the
Resize an Existing Rectangle, Ellipse, Highlighter or Area Measure Annotation ..................... 223
Rotate Text Annotations ........................................................................................................................ 223
Format Annotations ............................................................................................................................... 223
Delete Annotations ................................................................................................................................ 224
Synchronize Annotations .......................................................................................................................... 225
Measurement Tools .................................................................................................................................. 226
Line Measure ......................................................................................................................................... 226
Area Measure ........................................................................................................................................ 226
Angle Measure ...................................................................................................................................... 227
Layer Actions ............................................................................................................................................ 228
Multi Image Actions ............................................................................................................................... 229
Multi-Image Actions ................................................................................................................................... 229
Background Filters ................................................................................................................................. 229
Background Correction .......................................................................................................................... 229
Points of an Existing Line, Length Measure or Angle Measure Annotation ......... 223
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Table of Contents
Background Subtraction ........................................................................................................................ 229
Image Compositing ................................................................................................................................... 230
Create a Composite Image.................................................................................................................... 230
Player (Image Sequences)........................................................................................................................ 231
Purpose of the Player ............................................................................................................................ 231
Using the Player .................................................................................................................................... 231
Merge Functions .................................................................................................................................... 232
Player Features ..................................................................................................................................... 233
Player Options ....................................................................................................................................... 233
Extract or Delete individual Image files (frames) ................................................................................... 234
Saving .AVI Files ................................................................................................................................... 235
Create Templates ...................................................................................................................................... 236
Set-Up Hardware Master Templates ........................................................................................................ 236
Create a New Template ......................................................................................................................... 236
Edit, Synchronize and Delete Templates .................................................................................................. 238
Edit a Template ..................................................................................................................................... 238
Synchronize a Template ........................................................................................................................ 238
Delete a Template ................................................................................................................................. 238
Open and Save Images ............................................................................................................................ 239
Open and Save Images ............................................................................................................................ 239
Open Images ......................................................................................................................................... 239
Open a Previously Saved Image .................................................................................................... 239
Open a Demo Image ........................................................................................................................ 239
Save Images .......................................................................................................................................... 239
Save Using a Different File Folder, Name or Type ....................................................................... 240
Image File Types ................................................................................................................................... 240
Print Reports ............................................................................................................................................. 242
Print ........................................................................................................................................................... 242
File Print Command .................................................................................................................................. 243
Print Image History ................................
Reports .................................................................................................................................................. 244
Preview and Print a Report.................................................................................................................... 244
Print an Image ....................................................................................................................................... 245
Support 21 CFR Part 11 Compliance ....................................................................................................... 246
Supporting 21 CFR Part 11 Compliance ................................................................................................... 246
Purpose ................................................................................................................................................. 246
Features................................................................................................................................................. 246
Usage .................................................................................................................................................... 246
View an Image History Audit Trail .................................................................................................. 246
Print an Audit Trail (History) ........................................................................................................... 247
Image History ............................................................................................................................................ 249
View Image History ................................................................................................................................ 249
Add Notes in the Image History ............................................................................................................. 249
Glossary .................................................................................................................................................... 250
.................................................................................................... 244
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LS Software User Guide
Glossary .................................................................................................................................................... 250
Index .......................................................................................................................................................... 253
xii
Welcome
Welcome to LS Software Guide
UVP's Life Science (LS) software allows users to acquire, enhance, analyze and document images in a
simple and efficient way. Plus generate extensive reports and export them to Excel.
Life Science software is available as four unique configurations:
• VisionWorks®LS Acquisition
• VisionWorks®LS Acquisition and Analysis (includes 1D, Area Density and Colony Counting
Analysis)
• Doc-It®LS Acquisition
• Doc-It®LS Acquisition and Analysis (includes 1D and Colony Counting Analysis)
If a software function is grayed out, the function is not available with the version of software loaded on the
user’s computer.
The LS software is designed to image electrophoresis gels (DNA, RNA, Protein), blots, membranes,
plates, plants, and animals. Once an image has been captured with an application-specific camera, it can
be saved for documentation and presentations, manipulated for analysis, and annotated to point out key
features in the image.
What's New in Version 8
LS software release 8 new features include:
• New master template interface
• Updated workflow user interface
• Windows 7 and 8 compatibility
• Image interpolation for increased resolution
• Enhanced auto exposure capabilities
• Automatic histogram adjustment
• Automatic noise subtraction
• Automatic dark frame subtraction
Getting Started
• Minimum System Requirements
• Registering the Software
• User Administration
• Configure User Accounts
1
LS Software User Guide
Capturing Images
• Acquisition
Performing Analysis Functions on Images
• 1D Analysis
• Area Density
• Counting Colonies
2
Minimum System Requirements
Operating System Requirements
• Windows XP Professional with Service Pack 2 or higher, Windows 7, 8 and Vista
• Internet Explorer 8 or higher [To determine the version of Internet Explorer, open Internet
Explorer and click on Help > About]
• Intel Pentium Processor or equivalent, 1.6 GHz or higher
• 2 GB of RAM or greater (4 GB recommended)
• 200 MB of available hard disk space for the program, more for data
• To avail the functionality of 21 CFR Part 11 support, the disk partition must be formatted with
NTFS.
• CD-ROM drive
• Color monitor, supporting at least 1024 x 768 resolution and 16-bit or better colors; 24-bit or 32-
bit color is recommended
Welcome
• Computer equipped with minimum of three USB ports; additional ports required for peripheral
equipment (mouse, keyboard, etc.)
Note: Firewalls may impede successful installation and use of LS software, specifically for networked
applications. Contact your organization’s IT or network administrator to determine if a firewall or other
protection needs to be modified prior to installation of the software.
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LS Software User Guide
Registering the Software
• Overview
• Types of Registration
• On the Fly Activation
• Already Have an Activation ID
• Register Offline
• Single-user license
• Network-user license
• User Administration
Overview
The software is activated by entering an activation code provided by UVP in order to gain full access
rights to the software.
Once the software is installed, it will operate in full-feature trial mode for 14 days. Within the 14-day trial
mode, the software must be registered with UVP. Otherwise the software will only operate in
demonstration mode after the 14-day trail period. The demonstration mode limits the software to only
open and use the demonstration images provided by UVP. There are three ways to retrieve an activation
code: phone, email or Internet. All three methods are accessed via the License Wizard window.
• On the Fly Activation: Choose this option to activate the software immediately through the
Internet.
• Already Have an Activation ID: This option is useful when reloading (or upgrading) the software
after receiving an initial activation code.
• Offline Activation: This option is used if the computer is not connected to the Internet. This
allows the user to obtain the activation code and enter it at another time
Types of Registration
On the Fly Activation
To immediately activate the software through the Internet:
• Choose On the fly activation. If the computer is not connected to the Internet, follow the
instructions for Offline activation or call UVP to register the software.
• Click Next to continue.
4
• A new form will appear. Complete all required information on the form.
Welcome
• Fill out the Serial Number located on the CD. The number should be four sets of six numbers.
• Click the Get Activation No. button and then click onto Activate when the Activation Number
appears in the box.
Already Have an Activation ID
• Select Already have an activation ID from the buttons. This activation function is useful when
reloading the software after receiving an initial activation code.
• Click Next to continue.
• Click the link provided and complete the form to obtain instructions.
• Click Finish.
Offline Activation
• Select Offline Activation if the computer is not connected to the Internet. This allows the user to
obtain the activation code and enter it at another time.
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LS Software User Guide
• Click Next to continue.
• Click the link provided and complete the form to obtain instructions.
• Click Finish.
Next: Single-User License or Network-User License
6
Welcome
Single-User License
Once the software is registered, define the single-user license which allows the software to be used on a
single computer.
A Welcome to the Licensing Wizard window will appear.
• Select the Single client access license option.
• Select Next.
Next: If interested in 21 CFR Part 11 Compliance please set up login names and passwords by clicking
here (User Administration).
Related topics:
• Registering the software
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LS Software User Guide
Network-User License
A network-user license is only available for VisionWorks software. Once the software is registered, define
the network-user license which allows five users to operate the software. All licenses must run on the
same network. For additional licenses, contact UVP.
Note: Firewalls may impede successful installation and use of LS software, specifically for
networked applications. Contact your organization’s IT or network administrator to determine if a
firewall or other protection needs to be modified prior to installation of the software.
Follow these steps to install and use the network license:
• Ensure that a computer administrator is available to support the installation of the network
license. The administrator must provide an administrative login for each local PC installing
VisionWorks.
• Identify one computer running a minimum of Windows XP (SP2) on the network to hold the
network license. This computer will be called the "Server".
• From the VisionWorksLS software Disk 1, navigate to the License Server folder.
• In the License Server folder, select the Setup.exe to begin the Network License configuration.
• After selecting the Setup.exe, a UVP license server window will enable an Installation Wizard to
assist with the installation process. Select Next to continue.
8
• Click Next to confirm the installation of the License Server.
Welcome
• The next screen will require the user to enter an Activation Number. To get an activation number,
register at http://reg.uvp.com or call technical support at (800) 452-6788 (inside USA) or (909)
946-3197 . Enter the provided Activation Number.
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LS Software User Guide
• Click Next.
• The next screen will verify the "Port" number to be used. It will be necessary to note this port
number, as this is the port that the other/client PC’s will use to access the software license.
Generally, the port number is automatically provided and will not need to be altered.
• Click Next after noting the Port number being used.
NOTE: Administrative IT support will need to ensure that the port chosen is not blocked on the
network. If the network has a restrictive network use policy (blocked websites, no IM clients) port
blocking is likely to be implemented.
• The next screen will display the configuration status as it is applied. When the configuration is
complete, the text at the bottom portion of the window will read "Configuration complete." Click
Finish when this message appears.
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Welcome
• Next it will be necessary to obtain the "Server" computer’s IP Address. This address will be used
to set-up the other "client" computers, so that they will know where to locate the VisionWorks LS
Network License. Click the Windows Start button and select the Run option.
NOTE: "Client" computers are computers within the network that will run VisionWorksLS;
therefore a "Server" computer may also be a "Client" computer.
• In the Run dialog box, type cmd (as pictured). Then click OK.
• Type the command to display the IP Address of this PC. Type "ipconfig" (as pictured), and click
Enter.
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LS Software User Guide
• The IP Address is listed and should be noted, as it will be used with every "client" computer’s
License Manager setup. After noting the IP Address, close the Command Dialog.
NOTE: In some cases users will notice a list of multiple IP addresses. If the shipped system
includes an OptiChemi 600 camera, for example, users will see more than one IP address. To
ensure that the correct IP address is recorded, check with the IT Administrator before proceeding.
The IT Administrator must verify that the selected IP address corresponds to the network adapter
with access to the domain.
Additional Network Users
Repeat the following steps for each client computer.
• Open VisionWorksLS on the client computer. A Welcome to the Licensing Wizard window will
appear. Select the Server based licensing option, and select Next.
NOTE: VisionWorksLS will not authenticate across network segments so the IT Administrator
must
• Ensure that all client PC’s are on the same subnet/segment before installing VisionWorks on
each PC.
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Welcome
• Enter the previously noted IP Address and Port Number form the ”r;Server” computer. Click
Finish once the two values have been entered.
• UVP window will appear if the License Server installation/ configuration was successful. Click OK,
and close any other dialogs still open.
NOTE: During the installation process, a Systems Settings Change window may appear that asks
the user to restart the computer. This is not an error. Click No to continue with installation.
NOTE: The Hardware Installation window suggests that the camera drivers have not passed the
Windows Logo Testing suggested by Microsoft, which is not a problem. Click Continue Anyway.
• DO NOT SELECT STOP Installation. Doing so may render the cameras inoperable.
Next: If interested in 21 CFR Part 11 Compliance please set up login names and passwords by clicking
here (User Administration).
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LS Software User Guide
User Administration
• About Secure User Accounts
• User Rights
• Configure User Accounts
About Secure User Accounts
The concept of User Accounts for individual users is central to the software by providing security of user’s
data from being tampered with by other users, accidentally or otherwise.
• LS system of usernames and passwords is not to be confused with the login required to open the
computer. LS users must provide a separate login and password to enter the LS software.
• Setting up user accounts is mandatory if support for CFR 21 Part 11 is required from the
software.
• If individual accounts are not required, create just one account for all users and give full
permissions to that account.
Enable Secure User Accounts
• Request a System Administrator to log into the Windows computer.
• Navigate to C:\Documents and Settings\All Users
• Locate the Application Data folder.
• If the Application Data folder is not present in the All Users window, go to the Tools menu and
select Folder Options. (If the Application Data is present, skip the next step.)
• A Folder Options window appears. On the View tab, select Show hidden files and folders.
Select Apply then OK.
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Welcome
Column Heading
Description
Date Created
Date the user name was created.
Last Login
Date of last login.
Login Count
Number of times the user has logged in.
Idle Time Lock
Indicates the maximum idle time. To change the idle time, click Tasks
View
Enables user to view images. To change this setting, click Tasks >
• Go to the UVPSettings folder inside the Application Data folder.
• Have the System Administrator select Allow for all permissions listed for each user or group for
either the UVPSettings folder, or for each file within the folder (depending on the Network
Security Policy where the LS software will reside).
Definitions of the User Administration Columns
User Name Unique Identification name for a particular user. This could be a name
or a word that makes it easier to identify the user.
> Set Idle Time. Zero means no idle time.
Password
Expiration Date
Date the password expires.
Edit Rights. Click or unclick the View option.
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LS Software User Guide
Change
Enables user to change images. To change this setting, click Tasks >
Has Admin.
Gives user administration permissions. To change this setting, click
Login Privileges
Rights
Install
Un-
Open
and Run
User
Use the
Standard/Power
Edit Rights. Click or unclick the Change option.
Rights
Tasks > Edit Rights. Click or unclick the Has Administration Rights
option.
Users Rights
Depending on the privileges for the user that has logged onto windows, the following rights are available
to that user for the software:
Install
Restricted X* X
X* X
Admin X X X X X
X = Supported rights.
Note that even though a user may be able to do things with the
software which are outside of this matrix, UVP neither recommends
it, nor supports it. For example, the user may try (successfully or
otherwise) to uninstall the software as a Power User, but UVP does
not provide support for problems arising during or due to that
action.
Related Topics:
Configure User Accounts
Administration
Camera
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