Uvp GDP-8000 User Manual

GDS-8000

Image Acquisition and Analysis System

Set-Up and User Manual

BIOIMAGING SYSTEMS

GDS-8000

Image Acquisition and Analysis System

Set-Up and User Manual

UVP, Inc. Ultra-Violet Products Ltd
2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road
[phone] or [phone] Cambridge CB4 1TG UK
Fax: [phone] [phone]
E-Mail: [e-mail] Fax: [phone]
E-Mail: [e-mail]

http:\\www.uvp.com

81-0145-02

WWW.UVP.COM US Technical Support: 1.800.452.6788
UK Technical Support: +44.0.1223.420022

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GDS-8000 BIOIMAGING SYSTEM

Table of Contents

1. Introduction

2. Safety Information

3. GDS-8000 System Set Up
A. Camera Stand

1. Assembly/Adjustment

B. Darkrooms

1. Mini Darkroom

2. UV/White Darkroom

3. EpiChemi II Darkroom
C. Software
D. Accessories

4. 8-bit monochrome camera
A. Camera
B. Assembly instructions
C. Zoom Lens

5. System Check Out
A. Camera configuration
B. Focusing

6. Image Capture Methodologies
A. Non-Fluorescent Gels
B. Fluorescent Gels

Appendix A – Troubleshooting

Appendix B – Maintenance

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SECTION 1

Introduction

Congratulations! You have selected the most comprehensive BioImaging
Systems (GDS) available on the market today. UVP strives to keep up with the
advancements in technology, and wishes to assist you in any way possible.

How to use this manual:

The following compilation is intended to help the user install and upgrade their
darkroom systems in a step by step procedure. The first section will discuss
setting up the computer hardware (computer boards, monitor, printer). Once you
have completed that step, we will guide you through your darkroom and camera
system. From there, the manual will outline software installation, including video
board settings and acquisition software installation. The final step is setting up
the capture

As there are several products available, please make sure you are following the
instructions for your particular system.

Important Features of your system:

1. CCD Camera

• GDS 8000 : 8-bit monochrome offers great image quality
with varying focus and multiple darkroom options
(UV/White, Mini Darkroom, EpiChemi II).

2. Computer

• Minimum specifications:

• Standard 8-bit camera operation.

- Pentium II processor (or equivelant)

- 1 GB Hard drive

- 16 MB RAM

- 3 ½-in floppy drive

- CD ROM drive

- 8 MB Video

3. LabWorks Software

• Combines powerful image capture capability with
sophisticated analysis options. Acquire, calibrate, and
quantitate molecular weights, intensities, mass, and object
counts on:

• DNA, RNA, and Protein gels

• Slides and specimen selections

• Colony, culture, and microtiter plates

• Dot and Slot blots

• Films and Membranes

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For EpiChemi II Darkroom customers:

4. EpiChemi II Darkroom

• Overhead 254 nm UV, 365 nm UV, and fluorescent white light

• Enables the user to view the setup of the experiment in the

darkroom while the darkroom door is open.

• Concealed viewer with UV blocking safety glass.

• Enables the user to view the experiment while in progress,

while protecting from UV exposure.

• Completely light tight darkroom.

• Allows for best analysis of experiments under low levels of

phosphorescence.

• Retractable, sliding transilluminator tray.

• Allows for easier facilitation of experiments.

• Five-Position Filter Wheel.

• The filter wheel allows the user to select the specific

bandpass wavelengths for the experiments the researcher
would like to view. This enables the user to increase the
contrast and optimize the view of their experiments under
different excitation wavelengths.

• 1 stained glass bandpass filter.

• 3 dichroic interference filters for greater contrast.

• 1 Visible/White light pass filter

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SECTION 2

Safety Information

Warning:

All UVP High Performance Transilluminators are powerful sources of
Ultraviolet (UV) radiation that can cause damage to unprotected eyes and
skin. Be sure all personnel in the area are properly trained and protected
before operating any unit that uses UV radiation.

It is further recommended that the transilluminator be installed and operated in a
darkroom where access and exposure is limited while the unit is in operation. If a
darkroom is unavailable, UVP offers a wide selection of darkrooms or a shroud,
which provide protection from accidental exposure. For your safety and for the
safety of those around you, the transilluminator in a UVP darkroom is controlled
through the darkroom, and each darkroom is equipped with a safety switch that
controls the power to the transilluminator to prevent accidental exposure.

Each transilluminator is shipped with an ultraviolet blocking cover. Even though
the cover blocks out the UV radiation emitted by the unit, we recommend the
usage of UV blocking eyewear for additional protection.

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UK Technical Support: +44.0.1223.420022

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Section 3

GDS-8000 System Set Up

IF YOU RECEIVED YOUR BIOIMAGING SYSTEM WITH A COMPUTER
DIRECTLY FROM UVP, PLEASE SKIP TO SECTION 4: DARKROOM AND
CAMERA SYSTEMS

A) CAMERA STAND

A. Crank for vertical adjustment
B. Setscrew for vertical adjustment
C. Crank for adjustment of the camera bracket
D. Setscrew for camera bracket
E. Camera platform with rubber lining and line pattern for correct positioning of

the camera
F. ¼” camera screw
G. adjustment screws
H. Sliding carrier
I. Camera bracket
J. Adjustable camera arm

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ASSEMBLING INSTRUCTIONS

1. Mount the column on the baseboard, using the screws, washers and wing

nuts, (see drawings A and B).

2. Mount the two cranks on the hexagonal studs in such a way that the pin of the

studs engages in the recess of the cranks, (see drawing C).

1

2

ADJUSTMENT

1. Vertical adjustment is by means of crank “A”; the sliding carrier “H” is fixed in

position with Setscrew “B”.

2. Horizontal adjustment of the camera bracket “J” is done by crank “C”; it is

fixed in position with Setscrew “D”.

3. The recessed adjustment screws “G” are used to control smooth movement

of the horizontal and vertical adjustment. An Allen type wrench is required to

turn screws.

MOUNTING THE CAMERA

1. Unpack the camera and fixed lens

from their protective boxes and

remove the protective plugs.

2. Screw the fixed lens onto the end of

the C-mount of the camera. Make

sure that the UV filter is on the end

of the lens if doing Ethidium

Bromide work.

QUICK SNAP-ON MOUNT (#1 in picture C above)

1. Place the triangle onto the Adjustable Camera Arm “J” and tighten the bolt.

2. Detach the recessed square with the mounting screw by disengaging the

locking lever.

3. Screw the camera onto the side that has one mounting hole. Secure the

connection well. Do not worry about the spring-loaded tab.

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4. With the camera facing the baseboard insert the bottom side of the square
into the bottom of the recessed square on the camera arm. Push in. It
should snap and secure into place.

ADJUSTABLE CAMERA TRIANGLE (#2 in the picture C above)

1. Place the triangle onto the Adjustable Camera Arm “J” and tighten the
bolt.

Position the camera lens down and serial numbers facing out. Reach around,
locate, and tighten the securing bolt of the camera to the mount. Adjust as
necessary.

B) Darkrooms

The GDS-8000 system is a flexible system allowing users to choose from 3
darkroom options to use alongside an 8 bit non-cooled CCD camera:

The Mini Darkroom is designed to digitally document test results within the

laboratory. By placing a “benchtop” style transilluminator beneath the
darkroom, you are able to capture low-light fluorescent and visibly stained
media of various types with brightness and clarity.

The UV/White Darkroom is designed to digitally document results within the

laboratory. By placing either a standard format, UV/White, or a high
performance transilluminator beneath the darkroom, you are able to
capture low-light level media of various types with brightness and clarity.
The UV/White darkroom allows for use with the UV/White combination,
and larger transilluminators.

The EPICHEMI II Darkroom is designed specifically for low light and

fluorescence imaging. The special design incorporates a convenient
accessible sliding tray for a “benchtop” transilluminator. Its many dynamic
features make this the darkroom suitable for most every imaging
application.

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UK Technical Support: +44.0.1223.420022

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