UVP EpiChemi II Darkroom User Manual

EpiChemi II Darkroom

Set-Up & Operating
Instructions

IMPORTANT:

Please read these instructions before setting up and operating your

EpiChemi II Darkroom to familiarize yourself with operation procedures.

81-0202-01

EpiChemi II Darkroom

Table of Contents

Introduction ...................................................................................... 1

Components ................................................................................... 2

Set-Up and Operating Instructions................................................... 3

Troubleshooting .............................................................................. 5

Replacement Parts ......................................................................... 5

Technical Assistance ...................................................................... 6

Wiring Diagram .............................................................................. 7

EpiChemi II Darkroom

Introduction

The EpiChemi II (EC2) Darkroom is designed specifically for low light and fluorescence im­aging and because of its light-tightness is particularly suitable for chemiluminescent applica­tions. The special design accommodates a Benchtop Transilluminator on the convenient sliding
tray for easy access. For chemiluminescent applications, the chemi sample tray can be low­ered for sample positioning. Additionally, the darkroom provides users added flexibility due
to the filter wheel and overhead UV.

UVP offers a large selection of cameras with specially designed mounts for securely con­necting the camera to the darkroom. Camera specifications are not discussed in this manual.
Contact UVP or see other documentation included with your system for camera operating
and set-up instructions. Transilluminators are ordered separately and also include their own
documentation.

The EpiChemi II Darkroom features:

Overhead Light
Selector:
White Light
365nm UV
254nm UV

CCD Camera and Mounting Bracket

(Contact UVP or see other documentation
for camera set-up instructions. The
EpiChemi Darkroom may be sold with
various camera options with appropriate
mounting brackets.)

Filter Wheel
Selector

Transilluminator
Control

Master On/Off Switch

Gel Viewer

Compression
Door Lock

Benchtop UV
Transilluminator

Fold-Down
Sample Platform
for Chemi blots

Sliding Transilluminator Tray

1

EpiChemi II Darkroom

Components

The EpiChemi II Darkroom is comprised of:

Darkroom Cabinet

The EpiChemi II Darkroom is manufactured of stainless
steel construction and fabricated to provide a light-tight
chamber. The darkroom is ready to go with connections to
a wall outlet, the camera, mount and transilluminator.

Filter Wheel

The five-position filter wheel is designed for viewing many
fluorescent and visible color stains. Rotate the dial to de­sired filter as required for you application. The filter wheel is
securely mounted into the darkroom for a clean, protected
environment.

Filter Wheel

EC2 Darkroom Filter Performance

Gel Viewer

The viewer opens for safe viewing of samples. The safety
glass allows viewing without exposure to ultraviolet radia­tion.

Sample Platform

The sample platform folds down for placement of chemi
blots.

2

Sample Platform and
Transilluminator Tray

EpiChemi II Darkroom

Transilluminator Tray

The slides forward and back for easy access to the transilluminator surface. See transillu­minator documentation for specific transilluminator operating instructions.

NOTE: When using the transilluminator with the darkroom door open, UV exposure safety
precautions, including protective eyewear and clothing, must be observed to prevent harm­ful ultraviolet radiation exposure.

Set-Up and Operating Instructions

DO NOT ATTEMPT TO CONNECT ANY WIRING WHILE THE EQUIPMENT IS
CONNECTED TO ANY POWER SUPPLY.

CAUTION!

Do not install the EpiChemi II Darkroom in places with high moisture, dust or high
temperature.

Do not use any oil or petroleum based cleaner for the cabinet. Use only mild soap or
detergent solution for cleaning. Ensure that the system is turned OFF during cleaning.

Set-Up

1. Connect the camera and mount to the darkroom as
specified in the camera documentation manual.

2. Connect the power cord to the main power port which is
located on the back-top of the darkroom cabinet.

Operation

1. Turn on the master power switch on the front of the
darkroom cabinet. Once the power is switched on, power
is applied to all internal components and to the power port
for the jumper power cable that supplies power to the
transilluminator.

2. The Transilluminator Control knob has three options:

Safety Switch - Transilluminator safety control switch
automatically turns the transilluminator off when door opens
which prevents the user from exposure to UV

Always Off - This button setting puts the transilluminator in
Off mode. Use this switch when the transilluminator is not
required such as when capturing chemi blots.

3

EpiChemi II Darkroom

Always On - This setting overrides the safety switch with
the transilluminator always in the on mode. Users should

wear protective clothing and eyewear when the
darkroom is in the Always On mode to prevent exposure
to the UV.

3. The Overhead Light control knob has four options for
providing overhead diffused 254 and 365nm UV and
fluorescent white light for uniform epi-illumination.

Off - Place the overhead light switch in the Off mode when
not using the darkroom for applications requiring epi­illumination.

White - Overhead white light illuminates when white is
selected.

365nm and 254nm - When either ultraviolet wavelength is
selected, the red light will illuminate to alert the user that
the light is in operation. The overhead lights are designed
to automatically turn off when the darkroom door is open.

4. The Transilluminator Tray accommodates a Benchtop
UV Transilluminator. The tray moves forward or back for
easy access to the transilluminator surface.

NOTE: When using the transilluminator with the darkroom
door open, UV exposure safety precautions, including pro­tective eyewear and clothing,

must be observed to prevent

harmful ultraviolet radiation exposure.

5. The Sample Platform folds backwards against the back
of the darkroom cabinet. Pull the platform down into a
horizontal position as shown in the picture. The sample
platform is designed for use when transillumination is not
required. The platform provides a surface for placement of
chemiluminescent blots with minimum background noise
interference.

6. The Gel Viewer has a pressure-sensitive clasp. Press firmly
to open the viewer. The glass is UV blocking while providing
a clear view to the transilluminator or platform surface for
visibility of samples without opening the door.

4

EpiChemi II Darkroom

Troubleshooting

No power to darkroom cabinet.

1. Recheck main power cord connections to the both the EC2 Darkroom and the wall

power (surge protector).

2. Check the fuse located on the main power port. If the darkroom continues to blow

fuses, call UVP Technical Support Department.

Transilluminator will not turn on!

1. Be sure the darkroom cabinets door is completely closed. There is a UV exposure

safety cut-off switch that turns the transilluminator of when the darkroom cabinets door
is opened.

2. Be sure the darkroom cabinets main power switch is lit. If not, refer to No power to

darkroom cabinet above.

3. Be sure the transilluminator switch is on. When the switch on the transilluminator is lit

green, the unit is receiving power!

4. Be sure the transilluminator control switch on the darkrooms front panel is selected to

either safety switch or always on.

5. Call UVP Technical Support Department with any technical questions.

Replacement Parts

To order replacement parts for the EpiChemi II Darkroom, contact UVPs offices. Contact
information is listed on the next page.

Part Description Part Number Qty.

Tube, 8 watt, fluorescent, cool white 34-0056-01 2
Tube, 8 watt, 365nm UV longwave 34-0006-01 2
Tube, 8 watt, 254nm UV germicidal 34-0007-01 2
Cable and connector wall plug, 115V and 100V 58-0085-01 1
Cable and connector wall plug, 230V 58-0085-03 1

5

EpiChemi II Darkroom

Technical Assistance

UVP offers technical support for all of its products. If you have any questions about the
products use, operation or repair, please call or fax UVP Customer Service or Technical
Support at the following:

If you are located in North America, South If you are in Europe, Africa, the Middle East
America, East Asia or Australia: or Western Asia:

Call [phone] or [phone] and Call [phone] and ask for BioImaging

ask for BioImaging Systems Technical Support. Systems Technical Support. Support is available
Tech Support assistance is available during during regular business days, between 8:00am
regular business days, between 7:00am and and 5:30 pm UK time.
5:30 pm PST.

E-Mail your message to: E-Mail your message to
[e-mail] [e-mail]

Fax your questions to: Fax your questions to:
[phone] [phone]

Write to: Write to:

BioImaging Systems Technical Support BioImaging Systems Technical Support
UVP, Inc. Ultra-Violet Products Ltd.
2066 W. 11th Street Unit 1, Trinity Hall Farm Estate, Nuffield Road
Upland, CA 91786 USA Cambridge CB4 1TG UK

A Returned Goods Authorization (RGA) number must be obtained from UVPs
Customer Service Department prior to returning any product to UVP.

6

EpiChemi II Darkroom

Wiring Diagram

7

Products available from UVP

LabWorks Image Acquisition and Analysis Software

Ultraviolet Transilluminators

UV/White Light Transilluminators

Visi-Blue Transilluminators and Plates

White Light Transilluminators and Plates

Gel Tools

UV Crosslinkers

UV Viewing Cabinets

UV Lamps

Ultraviolet Intensity Meters

Hybridization Ovens

http://www.uvp.com

UVP, Inc., 2066 W. 11th Street, Upland, California 91786

[phone]  [phone] Fax: [phone]  E-Mail: [e-mail]

Ultra-Violet Products Limited, Unit 1, Trinity Hall Estate, Nuffield Rd. Cambridge, CB4 1TG UK

[phone]  Fax: [phone] E-Mail: [e-mail]