UVP EC3 Imaging System User Manual

EC3 Imaging System
Set-Up & Operating Instructions
UVP, LLC Ultra-Violet Products Ltd.
2066 W 11th Street, Upland, CA Unit 1, Trinity Hall Farm Estate, Nuffield Rd. Tel: (800) 452-6788 / (909) 946-3197 Cambridge CB4 1TG UK Fax: (909) 946-3597 Tel: +44(0)1223-420022 / Fax: +44(0)1223-420561
Web Site: www.uvp.com
EC3 Imaging System
Table of Contents
Introduction ......................................................................................1
Components ................................................................................... 2
Set-Up and Operating Instructions................................................... 3
Troubleshooting .............................................................................. 6
Repairs and Replacement Parts ..................................................... 7
Technical Assistance....................................................................... 8
EC3 Imaging System
Introduction
The EC3 Imaging System is designed specifically for low light and fluorescence imaging and because of its light-tightness is particularly suitable for chemiluminescent applications. The special design accommodates a Benchtop Transilluminator on the convenient sliding tray for easy access. For chemiluminescent applications, the chemi sample tray can be lowered for sample positioning. Additionally, the darkroom provides users added flexibility due to the filter wheel and overhead UV.
UVP offers a large selection of cameras with specially designed mounts for securely con­necting the camera to the darkroom.
The EC3 Imaging System features:
CCD Camera and Mounting Bracket
The EpiChemi Darkroom may be include with various camera/bracket options than shown in the photo. Refer to separate camera documentation.
Overhead Light Selector
White Light 365nm UV 254nm UV
Gel Viewer
Emission Filter Wheel Selector
Transillumination Controls
UV Transilluminator and Timer White Light Plate
Master On/Off Switch
Fold-down White Light Plate;
Place the Chemi Tray over the Plate for viewing blots
UV Transilluminator
Sliding Transilluminator Tray
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EC3 Imaging System
Components
Darkroom Cabinet
The EC3 Imaging System is manufactured of stainless steel con­struction and fabricated to provide a light-tight chamber. The darkroom is ready to go with connections to a wall outlet, the camera and cam­era mount and transilluminator and connection to a computer. As shown on page 1, components of the darkroom include:
Emission Filter Wheel
The five-position emission filter wheel is designed for viewing many fluorescent and visible color stains. Rotate the dial to desired filter as required for your application. The filter wheel is securely mounted into the darkroom for a clean, protected environment.
Filter Performance
Emission Filter Wheel Control
Overhead Light
UV and white light epi-illumination is built into the darkroom. Select from the white light, 365nm or 254nm UV.
Transilluminator Tray
The tray slides forward and back for easy access to the transillumina­tor surface. See transilluminator documentation for specific transillu­minator operating instructions.
NOTE: When using the transilluminator with the darkroom door open, UV exposure safety precautions, including protective eyewear and cloth­ing, must be observed to prevent harmful ultraviolet radiation expo­sure.
Transillumination Controls
The transilluminator and white light plate are controlled by the dark­room control panel. A timer is included for automatic transilluminator shutoff.
Overhead Light Control
Sample Platform, White Light Plate and Transilluminator Tray
Transillumination Controls
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EC3 Imaging System
Gel Viewer
The viewer opens for safe viewing of samples. The safety glass al­lows viewing without exposure to ultraviolet radiation.
Sample Platform and White Light Plate
The Sample Platform folds backwards against the back of the dark­room cabinet. A White Light Plate is built into the platform and is covered by a cover plate when the white light is not required.
Set-Up and Operating Instructions
Gel Viewer
Sample Platform, White Light Plate and Transilluminator Tray
NOTE: DO NOT ATTEMPT TO CONNECT ANY WIRING WHILE THE EQUIPMENT IS CONNECTED TO ANY POWER SUPPLY.
CAUTION!
Do not install the EC3 Imaging System in places with high moisture, dust or high temperature.
Do not use any oil or petroleum based cleaner for the cabinet. Use only mild soap or detergent solution for cleaning. Ensure that the system is turned OFF during cleaning.
Set-Up
1. Connect the camera and mount to the darkroom as specified in the camera documentation manual.
2. Connect the power cord to the main power port which is located on the back-top of the darkroom cabinet.
3. For shipping purposes, the chemi tray/white light platform is secured to the back wall of the darkroom to prevent movement in shipping.
To release the platform, use a Philips head screwdriver to remove the screws on the two sides of the platform.
Screws
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EC3 Imaging System
Operation
1. Turn on the master power switch on the front of the darkroom cabinet. Once the power is switched on, power is applied to all internal components and to the power port for the jumper power cable that supplies power to the transilluminator.
2. The Transilluminator Control knob has five options:
White Light On - Operates the white light plate located on the platform UV Safety - Transilluminator safety control switch automatically turns
the transilluminator off when door opens and prevents the user from exposure to UV
UV Always Off - This button setting puts the transilluminator in Off mode. Use this switch when the transilluminator is not required such as when capturing chemi blots.
UV Always On - This setting overrides the safety switch with the transilluminator always in the on mode. Users should wear
protective clothing and eyewear when the darkroom is in the Always On mode to prevent exposure to the UV.
Timer Switch - The timer operates the transilluminator. It is automatically set to shut off at 10 minutes to reduce overexposure to samples. To override this function, push the timer button to off or press reset to start the timer again.
3. The Overhead Light control knob has four options for providing overhead diffused 254 and 365nm UV and fluorescent white light for uniform epi-illumination.
Off - Place the overhead light switch in the Off mode when not using the darkroom for applications requiring epi-illumination.
Transillumination Controls
Overhead Light Control
White - Overhead white light illuminates when “white” is selected. 365nm and 254nm - When either ultraviolet wavelength is selected,
the red light will illuminate to alert the user that the light is in operation. The overhead lights are designed to automatically turn off when the
darkroom door is open.
4. The Emission Filter Wheel is designed with five-positions for viewing samples with the 400 - 655nm emission filters. Use the interface center to select from:
Blue (400-600nm) Green (515-570nm) Gold (485-655nm) Red (570-640nm) Clear (empty)
Emission Filter Wheel Control
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EC3 Imaging System
5. The Transilluminator Tray accommodates a Benchtop UV Transil­luminator. The tray moves forward or back for easy access to the transilluminator surface.
NOTE: When using the transilluminator with the darkroom door open, UV exposure safety precautions, including protective eyewear and clothing, must be observed to prevent harmful ultraviolet radiation exposure.
6. The Sample Platform folds backwards against the back of the darkroom cabinet. Pull the platform down into a horizontal position as shown in the picture. The sample platform is designed for use when transillumination is not required. The platform provides a surface for placement of chemiluminescent blots with minimum background noise interference. the platform adjusts to various heights. Slide the platform up or down using two hands.
When white light is required, position the sample platform at the desired height and remove the cover plate to expose the white light.
7. The Gel Viewer has a pressure-sensitive clasp. Press firmly to open the viewer. The glass is UV blocking while providing a clear view to the transilluminator or platform surface for visibility of samples without opening the door.
Transilluminator Tray
Sample Platform and White Light Plate
Gel Viewer
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EC3 Imaging System
Troubleshooting
No power to darkroom cabinet.
1. Recheck main power cord connections to the both the dark-
room and the wall power (surge protector).
2. Check the fuse located on the main power port. If the darkroom
continues to blow fuses, call UVP Technical Support Depart­ment.
Transilluminator will not turn on.
1. Check to make sure the darkroom cabinet’s door is completely
closed. There is a UV exposure safety cut-off switch that turns the transilluminator off when the darkroom cabinet’s door is opened.
2. Check the darkroom cabinet’s main power switch is lit. If not,
refer to “No power to darkroom cabinet” above.
3. Be sure the transilluminator switch is on. When the switch on
the transilluminator is lit green, the unit is receiving power!
4. Be sure the transilluminator control switch on the darkroom’s
front panel is selected to either “safety switch” or “always on.”
5. Call UVP Technical Support Department with any technical ques-
tions.
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EC3 Imaging System
Repairs and Replacement Parts
For technical assistance, contact UVP’s offices listed in the Technical Assistance section. To order replacement parts, contact UVP’s Customer Service Department at the same locations.
Switch Replacement Procedures
The darkroom and transilluminator use the same power switch. To replace the power switch in the darkroom or transilluminator:
1. Unplug the darkroom and/or transilluminator from the power
source prior to removing the power switch.
2. To remove the power switch from the unit, use a thin flat head
screw driver to pry the switch out from housing. Be careful not to scratch the paint of the darkroom or transilluminator in the pro­cess.
3. Once the switch is removed, you will see a number of connector
wires plugged into the back of it. Normally there are two black and two white wires, with some exceptions. Pull one wire out of the old switch and put it into the same connector location on the new switch. Continue this process for each wire until all are connect to the new switch.
Switch Replacement Procedures
4. Push the new switch back into its position.
Replacement Parts
To order replacement parts for the EC3 Imaging System, contact UVP’s offices. Contact information is listed on the next page.
Part Description Part Number Qty.
Tube, 8 watt, fluorescent, cool white 34-0056-01 2 Tube, 8 watt, 365nm UV longwave 34-0006-01 2 Tube, 8 watt, 254nm UV germicidal 34-0007-01 2 Cable and connector wall plug, 115V and 100V 58-0085-01 1 Cable and connector wall plug, 230V 58-0085-03 1 Power Switch 53-0024-01 1
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EC3 Imaging System
Technical Assistance
UVP offers technical support for all of its products. If you have any questions about the product’s use, operation or repair, please call or fax UVP Customer Service or Technical Support at the following:
If you are located in North America, South If you are in Europe, Africa, the Middle East America, East Asia or Australia: or Western Asia:
Call (800) 452-6788 or (909) 946-3197 and Call +44(0) 1223-420022 and ask for BioImaging
ask for BioImaging Systems Technical Support. Systems Technical Support. Support is available Tech Support assistance is available during during regular business days, between 8:00am regular business days, between 7:00am and and 5:30 pm UK time. 5:30 pm PST.
E-Mail your message to: E-Mail your message to techsupport@uvp.com uvp@uvp.co.uk
Fax your questions to: Fax your questions to: (909) 946-3597 +44(0)1223-420561
Write to: Write to:
BioImaging Systems Technical Support BioImaging Systems Technical Support UVP, LLC Ultra-Violet Products Ltd. 2066 W. 11th Street Unit 1, Trinity Hall Farm Estate, Nuffield Road Upland, CA 91786 USA Cambridge CB4 1TG UK
NOTE! A Returned Goods Authorization (RGA) number must be obtained from UVP’s Customer Service Department prior to returning any product to UVP.
Products available from UVP
VisionWorksLS Image Acquisition and Analysis Software
Ultraviolet Transilluminators
UV/White Light Transilluminators
Visi-Blue Transilluminators and Plates
White Light Transilluminators and Plates
Gel Tools
UV Crosslinkers
UV Viewing Cabinets
UV Lamps
Ultraviolet Intensity Meters
Hybridization Ovens
PCR Workstations
UV Incubator
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