UVP DigiDoc-It Imaging System User Manual

DigiDoc-It® Imaging System

Installation and User Instructions

UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]

Web Site: www.uvp.com

81-0218-01 Rev L

DigiDoc-It Imaging System 2

Camera cap

camera

Handles for carrying

the hood

Gel Viewport

Window

Hood access door

DigiCam 125 camera

Ethidium Bromide Filter

Viewport cover

High Performance UV

Transilluminator

Introduction

The DigiDoc-It Imaging System is a basic gel imaging system designed to fit over any of UVP’s optional High
Performance UV Transilluminators or optional Benchtop UV Transilluminator and drawer combination. The
system combines:

 Hood enclosure
 DigiCam 125 digital color camera
 Ethidium Bromide (EtBr) Filter
 Doc-It
 UV transilluminator or drawer assembly (optional). Transilluminator instructions are provided in a

enclosure for
access to the

®

LS Acquisition Software (refer to software manual for installation)

separate manual.

is located inside the

hood under the camera

Hood Enclosure

The DigiDoc-It hood enclosure includes a cap to reduce ambient and
reflective light when taking pictures. The soft rubber viewport window
allows researchers to view samples prior to capturing images. The slide
closure under the viewport minimizes ambient light when taking
pictures. The lightweight door provides access to the enclosure interior
for placement of samples on the transilluminator surface.

The DigiDoc-It drawer fits under the hood enclosure and accommodates
a compact Benchtop UV Transilluminator.

DigiDoc-It with drawer

DigiDoc-It Imaging System 2

Doc-ItLS Acquisition Software

Camera & UV Filter

The digital color camera includes a zoom lens.
The EtBr filter, orange-colored UV blocking band pass filter, is installed inside the hood at the UVP factory. The

filter is used to absorb UV and IR radiation from the transilluminator and to enhance the orange/pink bands
generated by Ethidium Bromide stained gels. The filter can be removed when imaging non-fluorescent media
(protein gels, colony plates, etc.) in order to produce brighter images.

Doc-ItLS Software

The Doc-ItLS software loads onto the user’s existing computer. The software controls the camera acquisition
functions as well as enhancement and reporting features. For information on installation and use of the software,
refer to the Doc-ItLS Software help files.

UV Transilluminator

An UV transilluminator may be shipped with the system. The transilluminator includes an ultraviolet blocking
cover and the cover should be removed when the transilluminator is used with the system. Refer to the
Transilluminator manual for separate instructions.

Operating System Requirements

• Windows 7, Vista or XP SP2 or later

• Internet Explorer 6.0 or higher [To locate the version of Internet Explorer, open Internet Explorer and

click on Help->About Internet Explorer]

• Intel Pentium Processor or equivalent, 1.6 GHz or higher

• 1 GB of RAM or greater

• 200 MB of available hard disk space for the program, more for data

• CD-ROM drive

• One Universal Serial Bus (USB) for connecting the camera

• Color monitor, supporting at least 1024 x 768 resolution and 16-bit or better colors; 24-bit or 32-bit color

is strongly recommended

NOTE: For 21 CFR Part 11 support functionality, the computer partition must be formatted with NTFS. Refer to
the Doc-ItLS software manual for instructions.

DigiDoc-It Imaging System 3

High Performance 2UV Transilluminator

DigiDoc-It Drawer

(may be different color)

System Setup

DO NOT ATTEMPT TO CONNECT ANY WIRING W HILE THE EQUIPMENT IS CONNE CTED TO ANY POWER
SUPPLY.

CAUTION: Do not install the system in places with high moisture, dust or high temperature. Do not use any oil or
petroleum based cleaner for the cabinet. Use only mild soap or detergent solution for cleaning. Ensure that the
system is turned OFF during cleaning. Keep the equipment away from motors or other large magnetic equipment
apparatus.

Place the Hood on top of the Transilluminator

The Hood Enclosure can be used with a High Performance UV Transilluminator. When using a Benchtop
Transilluminator, the optional drawer is required.

CAUTION: All UVP Transilluminators are powerful sources of UV radiation that will cause damage to unprotected
eyes and skin. Before operating any unit, be sure all personnel in the area are properly protected. UV Blocking
Eyewear is highly recommended and should be worn. Refer to the Accessories section of this manual for UV
Blocking Eyewear.

High Performance Transilluminator

1. Remove the UV blocking clea r c over from the
Transilluminator.

2. Position the transilluminator so the switch is facing
the user.

3. Connect the power cord to the back of the
transilluminator and connect the plug to a power
source.

4. Place the Hood Enclosure on top of the
Transilluminator surface so that the filter glass is
covered.

Benchtop Transilluminator and Drawer

1. Remove the UV blocking clear cover from the Transilluminator.

2. Place the Benchtop Transilluminator into the drawer.

3. Connect the power cord to the back of the transilluminator and
push the plug through the access hole in the back of the
drawer.

4. Connect the plug to the power source.

5. Access to the transilluminator switch is via the access port on
the front of the drawer.

6. Place the DigiDoc-it hood over the drawer.

Camera Assembly

(shown with UV blocking cover)

1. The camera will be installed in the DigiDoc-It hood at the UVP
factory.

2. To connect the power cable to the camera, thread the
camera’s power cable through the round notched hole from the
back of the camera cap and plug it into the camera as shown.