UVP DigiDoc-It Set Up And Operating Instructions Manual

DigiDoc-It™ Imaging System

Set-Up and Operating Instructions

UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]

81-0218-01 Rev. I

Web Site: www.uvp.com

DigiDoc-It Imaging System 2

Introduction

The DigiDoc-It Imaging System is a basic gel imaging system designed to fit over any of UVP’s optional High
Performance UV Transilluminators or optional Benchtop UV T r ansilluminator and drawer combination. The
system combines:

 Hood enclosure
 DigiCam 110 digital color camera, Ethidium Bromide (EtBr) Filter
 Doc-It
 UV transilluminator or drawer assembly (optional). Transilluminator instructions are provided in a

Camera cap
enclosure for
access to the
camera

Handles for carrying
the hood

®

LS Acquisition Software (refer to software manual for installation)

separate manual.

DigiCam 110 camera

Ethidium Bromide Filter
is located inside the
hood under the camera

Gel Viewport
Window

Hood access door

Hood Enclosure

The DigiDoc-It hood enclosure includes a cap to reduce ambient and
reflective light when taking pictures. The soft rubber viewport window
allows researchers to view samples prior to capturing images. The slide
closure under the viewport minimizes ambient light when taking
pictures. The lightweight door provides access to the enclosure interior
for placement of samples on the transilluminator surface.

The DigiDoc-It drawer fits under the hood enclosure and accommodates
a compact Benchtop UV Transilluminator.

Viewport cover

High Performance UV
Transilluminator

DigiDoc-It with drawer

DigiDoc-It Imaging System 2

Camera & UV Filter

The digital color camera (DigiCam photo below – actual camera may be different) includes a bui lt-in optical zoom
lens.

The EtBr filter, orange-colored UV blocking band pass filter, is installed inside the hood at the UVP factory. The
filter is used to absorb UV and IR radiation from the transilluminator and to enhance the orang e/pink bands
generated by Ethidium Bromide stained gels. The filter can be removed when imaging no n-fluorescent media
(protein gels, colony plates, etc.) in order to produce brighter images.

Doc-It®LS Acquisition Software

The Doc-ItLS software loads onto the user’s existing computer.
The software controls the camera acquisition functions as well as
enhancement and reporting features. For information on
installation and use of the software, refer to the Doc-ItLS
Software Manual (pdf file on the software CD).

Capture software

Transilluminator

An UV transilluminator may be shipped with the system. The transilluminator includes an ultraviolet blocking
cover and the cover should be removed when the transilluminator is used with the system.

Operating System requirements

Windows 2000 with Service Pack-4 or higher


 Windows XP Professional with Service Pack-2 or higher
 Internet Explorer 6.0 or higher [To locate the version of Internet Explorer, open Internet Explorer and

click on Help->About Internet Explorer]

 Intel Pentium Processor or equivalent, 1.6 GHz or higher
 1 GB of RAM or greater
 200 MB of available hard disk space for the program, more for data
 CD-ROM drive
 One Universal Serial Bus (USB) for connecting the camera
 Color monitor, supporting at least 1024 x 768 resolution and 16-bit or better colors; 24-bi t or 32-bit color

is strongly recommended

NOTE: For 21 CFR Part 11 support functionality, the computer partition must be formatted with NTFS. Refer
to the Doc-ItLS software manual for instructions.