UVP DigiDoc-It Set Up And Operating Instructions Manual

DigiDoc-It™ Imaging System

Set-Up and Operating Instructions

UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]

81-0218-01 Rev. I

Web Site: www.uvp.com

DigiDoc-It Imaging System 2

Introduction

The DigiDoc-It Imaging System is a basic gel imaging system designed to fit over any of UVP’s optional High
Performance UV Transilluminators or optional Benchtop UV T r ansilluminator and drawer combination. The
system combines:

 Hood enclosure
 DigiCam 110 digital color camera, Ethidium Bromide (EtBr) Filter
 Doc-It
 UV transilluminator or drawer assembly (optional). Transilluminator instructions are provided in a

Camera cap
enclosure for
access to the
camera

Handles for carrying
the hood

®

LS Acquisition Software (refer to software manual for installation)

separate manual.

DigiCam 110 camera

Ethidium Bromide Filter
is located inside the
hood under the camera

Gel Viewport
Window

Hood access door

Hood Enclosure

The DigiDoc-It hood enclosure includes a cap to reduce ambient and
reflective light when taking pictures. The soft rubber viewport window
allows researchers to view samples prior to capturing images. The slide
closure under the viewport minimizes ambient light when taking
pictures. The lightweight door provides access to the enclosure interior
for placement of samples on the transilluminator surface.

The DigiDoc-It drawer fits under the hood enclosure and accommodates
a compact Benchtop UV Transilluminator.

Viewport cover

High Performance UV
Transilluminator

DigiDoc-It with drawer

DigiDoc-It Imaging System 2

Camera & UV Filter

The digital color camera (DigiCam photo below – actual camera may be different) includes a bui lt-in optical zoom
lens.

The EtBr filter, orange-colored UV blocking band pass filter, is installed inside the hood at the UVP factory. The
filter is used to absorb UV and IR radiation from the transilluminator and to enhance the orang e/pink bands
generated by Ethidium Bromide stained gels. The filter can be removed when imaging no n-fluorescent media
(protein gels, colony plates, etc.) in order to produce brighter images.

Doc-It®LS Acquisition Software

The Doc-ItLS software loads onto the user’s existing computer.
The software controls the camera acquisition functions as well as
enhancement and reporting features. For information on
installation and use of the software, refer to the Doc-ItLS
Software Manual (pdf file on the software CD).

Capture software

Transilluminator

An UV transilluminator may be shipped with the system. The transilluminator includes an ultraviolet blocking
cover and the cover should be removed when the transilluminator is used with the system.

Operating System requirements

Windows 2000 with Service Pack-4 or higher


 Windows XP Professional with Service Pack-2 or higher
 Internet Explorer 6.0 or higher [To locate the version of Internet Explorer, open Internet Explorer and

click on Help->About Internet Explorer]

 Intel Pentium Processor or equivalent, 1.6 GHz or higher
 1 GB of RAM or greater
 200 MB of available hard disk space for the program, more for data
 CD-ROM drive
 One Universal Serial Bus (USB) for connecting the camera
 Color monitor, supporting at least 1024 x 768 resolution and 16-bit or better colors; 24-bi t or 32-bit color

is strongly recommended

NOTE: For 21 CFR Part 11 support functionality, the computer partition must be formatted with NTFS. Refer
to the Doc-ItLS software manual for instructions.

DigiDoc-It Imaging System 3

System Set-Up

DO NOT ATTEMPT TO CONNECT ANY WIRING WHILE THE EQUIPMENT IS CONNECTED TO ANY POWER
SUPPLY.

CAUTION: Do not install the system in places with high moisture, dust or high temperature. Do not use any oil or
petroleum based cleaner for the cabinet. Use only mild soap or detergent solution for cleaning. Ensure that the
system is turned OFF during cleaning. Keep the equipment away from motors or other large magnetic equipment
apparatus.

Place the Hood on top of the Transilluminator

The Hood Enclosure can be used with a High Performance UV Transilluminator. When using a Benchtop
Transilluminator, the optional drawer is required.

CAUTION: All UVP Transilluminators are powerful sources of UV radiation that will cause damage to
unprotected eyes and skin. Before operating any unit, be sure all personnel in the area are properly protected.
UV Blocking Eyewear is highly recommended and should be worn. Refer to the Accessories section of this
manual for UV Blocking Eyewear.

High Performance Transilluminator

Remove the UV blocking clear cover from the
Transilluminator.

Position the transilluminator so the switch is facing the
user.

Connect the power cord to the back of the transilluminator
and connect the plug to a power source.

Place the Hood Enclosure on top of the Transilluminator
surface so that the filter glass is covered.

Benchtop Transillumina

Remove the UV blocking clear cover from the Transilluminator.
Place the Benchtop Transilluminator into the drawer.
Connect the power cord to the back of the transilluminator and

push the plug through the access hole in the back of the drawer.
Connect the plug to the power source.
Access to the transilluminator switch is via the access port on the

front of the drawer.
Place the DigiDoc-it hood over the drawer.

tor and Drawer

High Performance UV Transilluminator

(shown with UV blocking cover)

DigiDoc-It Drawer

(may be different color)

DigiDoc-It Imaging System 4

Camera Assembly

To assemble the camera, plug the power cord into the
power supply.

Connect the cable from the power supply to the
battery.

Ensure that the camera is off before inserting the
battery. (The battery may need to be charged before
use)

To insert the battery, slide the battery cover open and
insert the battery until the battery lock clicks.

Close the battery door.
A memory card is required for capturing images. To

insert the memory card, slide the card slot cover open
and insert the memory card into the slot until the card
eject button extends.

Close the card slot cover.
Connect the USB cable to the camera but leave the

other end unplugged until ready to load the software.

Power supply cable
plugged into battery

USB cable

DigiCam 110
camera

Hex Nut

Power supply
cable

Power supply

Allen Wrench

Camera Installation

To install the camera, open the camera cap on the top of the hood.

Power cord

DigiDoc-It Imaging System 5

Position the camera on the top of the hood so that the lens fits through the gasket seal. Insert the hex screw

through the camera mounting bracket and turn the hex screw clockwise to tighten it into the camera’s tripod
mounting threads.

Pass the camera power cable through the round notched hole from the back the camera cap and plug into the
camera.

Plug the camera’s power supply into a wall outlet or surge-protector receptacle.

Note: The Ethidium Bromide filter is installed inside the hood enclosure at the UVP factory. If the filter needs to
be replaced, turn the hood upside down. Loosen screws and slide the filter into the filter tray. Retighten screws
and place the hood upright.

Install the Doc-ItLS acquisition software

Note: The following provides a brief instruction for installing the software. For additional instruction on using the

software, refer to the Doc-ItLS Software manual.
Insert the CD into the CD-ROM drive. The installation program should automatically start. If the installation

program fails to start, navigate to the CD-ROM drive and double-click setup.exe to launch DocIt-LS.
Follow instructions from the Wizard screens as they appear, clicking Next, Accept or Finish as appropriate.
If prompted to restart the computer, click No.
Restart the computer by choosing Restart from the Windows Start menu.
Double click onto the UVP icon on the desktop.
Activate the software by choosing On the fly activation if connected to the internet. Complete all the required

information on the form and fill in the Serial Number (located on the CD). Click onto Get Activation No. and
then click onto Activate when the Activation Numb er appears in the box.

Close the software.

Note: If not connected to the internet, call technical services at [phone] / [phone], to complete
the activation process.

Connect the camera to the computer

Note: it is recommended to only connect the

camera to the computer while the camera is off.
Connect the USB cable from the camera to the

computer.
Turn the camera on by selecting Auto on both

camera dials.
Open the DocIt-LS software. The software

should automatically identify the camera. The
camera control window should be active and
ready to capture images.

Camera set to Auto

DigiDoc-It Imaging System 6

System Operation

Camera Operation

It is recommended that the camera be turned off when not in use.

 To use the camera, open the camera cap on top of the hood.
 Set both camera mode dial buttons to Auto to turn on the camera.
 Close and secure the camera cap on the hood.
 If the camera is off when starting Doc-It software, a message stating that the application cannot find a

camera will appear. To remedy this, turn the camera on and click Retry.

 When exiting the Doc-It software, a reminder to turn the camera off to save battery life will appear.

Loading Gels into the Darkroom Hood

Turn Off the UV Transilluminator.
Place the gels onto the UV Transilluminator.
Place the BioDoc-It hood over the UV Transilluminator.
Ensure that the digital camera is on by turning the camera mode dials to Auto.
Turn On the transilluminator.
Use the Gel Viewport Window to view the positioning of the gels. (Slide away the Viewport cover to view the gel

and then replace the cover when ready to capture the image.)
Capture the image using appropriate software exposure settings.
Turn Off the transilluminator.
Note: It is important to minimize the light source exposure to the sample. Turn off the light source as soon as the

image is captured.

View a Sample Prior to Capture

Open the viewport on the hood by sliding the viewport cover to the right.
After visual checking that the sample is loaded correctly, close the viewport cover. Otherwise, light from the

viewing window will interfere with the capturing process.

Service Procedures

Return Procedure

A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service before
returning any product.

Replacement Parts and Accessories

Accessories are available for use with the DigiDoc-It Imaging Systems. Contact UVP’s offices at the telephone
numbers below for replacement parts for DigiDoc-It equipment. For transilluminator replacement parts, refer to
the separate manual for the transilluminators included with your shipment.

Description Part Numbers

Drawer (for Benchtop Transilluminators) 98-0068-01
Doc-ItLS Analysis Software 97-0185-02
Thermal Printer, Digital (115/230V) 89-0069-06
Thermal Printer, Digital (230V) 89-0069-07
Thermal Printer, Digital (230V for Europe) 98-0069-15

DigiDoc-It Imaging System 7

Thermal Paper, 4 rolls (800 images) 89-0038-01
Thermal paper, 16 rolls (3200 images) 89-0038-04
Thermal Paper, 40 rolls (8000 images) 89-0038-05

UV to White Light Converter Plate (21x26cm) 38-0191-01

Visi-Blue Converter Plate (21x26cm) 38-0200-01
Visi-Blue Converter Plate (20x40cm) 38-0200-02
Filter, 50mm, sq., SYBR Green and EGFP 38-0219-01
Filter, 50mm, sq., Ethidium Bromide 38-0220-01

UV to White Light Converter Plate (20x40cm) 38-0191-02

Technical Assistance

UVP offers technical support on all of its products. If there are questions about the product’s use, operation
or repair, please contact our offices at the locations below, or go to UVP’s web site and click the Tech
Support > BioImaging Systems.

If residing in North America, South

America, East Asia or Australia:

If residing in Europe, Africa, the Middle

East of Western Asia:

Warranty

UVP's products are guaranteed to be free of defects in materials, workmanship and manufacture for one (1)
year from date of purchase. Consumable and disposable parts including, but not limited to tubes and filters, are
guaranteed to be free from defects in manu facture and materia ls for nine ty (90) days fro m date o f purchase.
Transilluminators carry a two (2) year warranty from da te of pu rcha se. If equip ment fail ure or mal function
occurs during the warranty period, UVP shall examine the inoperative equipment and have the option of
repairing or replacing any part(s) which, in the judg men t of U VP, were originally defective or became so under
conditions of normal usage and service.

No warranty shall apply to this instrumen t, or part the reo f, that ha s been sub jec t to ac ciden t, negli gence ,
alteration, abuse or misuse by the end-user. Moreover, UVP makes no warranties whatsoever with respect to
parts not supplied by UVP or that have been installed, used and/o r serviced o ther than in strict compliance with
instructions appearing in this manual.

In no event shall UVP be responsible to the end-user fo r any incidental or consequential damages, whether
foreseeable or not, including but not limited to property damage, inability to use equipment, lost business, lost
profits, or inconvenience arising out of or connected with the use of instruments produced by UVP. Nor is UVP
liable or responsible for any personal injuries occurring as a resul t of the use, installation and /o r servicing of
equipment.

This warranty does not supe rsed e any statutory rights that may be avail able in certain coun trie s.

Call [phone] or [phone],
and ask for Customer Service during
regular business days, between 7:00 am
and 5:00 pm, PST.

E-mail your message to:
[e-mail]

Fax Customer Service, and send it to (909)
946-3597

Write to: UVP, LLC 2066 W. 11th Street,

Upland, CA 91786 USA

Doc-It is a registered trademark of UVP, LLC. DigiDoc-It is a trademark of UVP, LLC

Call [phone], and ask for

Customer Service during regular business

days between 9:00 am and 5:30 pm.

E-mail your message to: [e-mail]

Fax Customer Service, and send it to: +44(0)

1223-420561

Write to: Ultra-Violet Products Ltd

Unit 1, Trinity Hall Farm Estate, Nuffield Road,

Cambridge CB4 1TG UK