UVP ChromaDoc-It Imaging System User Manual

ChromaDoc-It™ Imaging System

Installation and User Instructions

UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]

Web Site: www.uvp.com

81-0295-01 Rev E

ChromaDoc-It Imaging System 2

ambient light

DigiCam 125 camera

with diopter

TLC Filter is located

Introduction

The ChromaDoc-It Imaging System is designed for capturing brilliant Thin Layer Chromatography (TLC) images.
The system combines:

 Viewing Cabinet with built-in epi white light and UV: 254nm (shortwave) and 365nm (longwave) UV
 DigiCam 125 digital color cam era
 Ethidium Bromide (EtBr) Filter
 Doc-It

Cap enclosure (not shown)

reduces external light

when taking pictures

®

LS Acquisition Software (refer to software manual for installation)

inside the camera

mount

Access curtain blo ck s

Overhead UV and white

light

Viewing Cabinet

The ChromaDoc-It Imaging System components include a Chromato-Vue viewing cabinet with epi 254nm and
365nm UV to fluoresce various stained gels. The cabinet provides white light for illuminating the cabinet interior.
For additional information about the cabinet, refer to the separate Chromato-Vue Cabinet manual.

UV Filter

The TLC, orange-colored UV blocking band pass filter, is installed inside the cabinet at the UVP factory. The filter
is used to absorb UV and IR radiation from the transilluminator and to enhance the orange/pink bands generated
by EtBr stained gels. The filter can be removed when imaging non-fluoresc ent med ia in order to produce brighter
images.

Camera and Diopter

The digital color camera includes a zoom lens. A close-up diopter is connected to the lens at UVP’s factory.

ChromaDoc-It Imaging System 3

Doc-ItLS Acquisition Software

Doc-ItLS Software

The Doc-ItLS software loads onto the user’s existing computer. The software controls the camera acquisition
functions as well as enhancement and reporting features. For information on installation and use of the software,
refer to the Doc-ItLS Software Manual (pdf file on the software CD).

Operating System Requirements

• Windows 7, Vista or XP SP2 or later

• Internet Explorer 6.0 or higher [To locate the version of Internet Explorer, open Internet Explorer and

click on Help->About Internet Explorer]

• Intel Pentium Processor or equivalent, 1.6 GHz or higher

• 1 GB of RAM or greater

• 200 MB of available hard disk space for the program, more for data

• CD-ROM drive

• One Universal Serial Bus (USB) for connecting the camera

• Color monitor, supporting at least 1024 x 768 resolution and 16-bit or better colors; 24-bit or 32-bit color

is strongly recommended

NOTE: For 21 CFR Part 11 support functionality, the computer partition must be formatted with NTFS. Refer to
the Doc-ItLS software manual for instru ctio ns.

ChromaDoc-It Imaging System 4

1. The camera will be installed in the ChromaDoc-It hood at the UVP factory.

Camera bracket

and mount

System Setup

DO NOT ATTEMPT TO CONNECT ANY WIRING W HILE THE EQUIPMENT IS CONNECTED TO ANY POWER
SUPPLY.

CAUTION: Do not install the system in places with high moisture, dust or high temperature. Do not use any oil or
petroleum based cleaner for the cabinet. Use only mild soap or detergent solution for cleaning. Ensure that the
system is turned OFF during cleaning. Keep the equipment away from motors or other large magnetic equipment
apparatus.

Position the Cabinet

Place the cabinet on a level surface with the curtain facing
forward. Plug the cabinet into a power outlet.

Camera Assembly

2. Connect the power cable to the camera.

3. Connect the USB cable to the camera. Leave the other end unplugged until ready to load the
software.

4. Plug the camera’s power supply into a wall outlet or surge-protector receptacle.

NOTE: The filter is installed at the UVP factory.

Install the Doc-ItLS Software

NOTE: The following provides a brief instruction for installing the software. For additional instruction on

using the software, refer to the Doc-ItLS help files.

1. Insert the CD into the CD-ROM drive. The installation program should automatically start. If the

installation program fails to start, navigate to the CD-ROM drive and double-click setup.exe to
launch Doc-It.

2. Follow instructions from the Wizard screens as they appear, clicking Next, Accept or Finish as

appropriate.

3. If prompted to restart the computer, click No.

4. Restart the computer by choosing Restart from the Windows Start menu.

5. Double click onto the UVP icon on the desktop.

6. Activate the software by choosing On the fly activation if connected to the internet. Complete all

the required information on the form and fill in the Serial Number (located on the CD). Click onto
Get Activation No. and then click onto Activate when the Activation Number appears in the box.

7. Close the software.

NOTE: If not connected to the Internet, call technical services at [phone] or [phone] to
complete the activation process.

ChromaDoc-It Imaging System 5

Connect the Camera to the Computer

NOTE: It is recommended to only connect the camera to the computer while the camera is off.

1. Connect the USB cable from the camera to the computer.

2. Turn the camera on and select A-DEP on the camera dial.

NOTE: Do not use the Doc-ItLS software while the camera dial is set to movie mode as doing
so may result in an error.

Camera set to A-DEP

Connect to Doc-ItLS Software

1. Open the Doc-ItLS software. The software should automatically identify the camera. The camera
control window should be active and ready to capture images.

NOTE: Additional instruction on using the software, refer to the Doc-ItLS Software help files.

ChromaDoc-It Imaging System 6

Using the System

Camera Operation

NOTE: Do not use the Doc-It soft ware while the camera dial is set to movie mode as doing so may result
in an error.

1. To use the camera, remove the camera cap from the cabinet.

2. Ensure that the camera is turned on.

3. Set the camera mode dial button to A-DEP. (Automatic mode is recommended for a quick image
capture)

4. Close and secure the camera cap on the cabinet.

5. If the camera is off when starting Doc-It software, a message stating that the application cannot find a
camera will appear. To remedy this, turn the camera on and click Retry.

Loading Gels into the Cabinet

1. Gels are loaded into the cabinet via the curtain in the front of the cabinet. Place the sample to be
imaged in the center of the cabinet floor.

2. Ensure that the digital camera is on.

3. Turn the camera mode dials to A-DEP.

4. Place the camera cap over the camera to eliminate ambient light.

5. Turn on the appropriate UV light source on the top of the cabinet.

NOTE: It is important to turn off the light source as soon as the image is captured to prevent photonicking or
damage to the gel.

NOTE: Refer to the help files for additional capture and enhancement instructions.

Software Operation

NOTE: Do not use the Doc-It software while the camera dial is set to movie mode, as doing so may result
in an error.

1. Ensure that the camera mode is set to A-DEP for automat ed capt ure.

2. Open the Doc-ItLS software.

3. A window displaying the camera options should appear as pictured below. If not, click on the Acquisition
Action tab and then on the Camera menu button.

Camera Control Window

ChromaDoc-It Imaging System 7

4. To take images in color, ensure that the Color Capture box is checked.

5. Select Preview at the top of the software to preview the image.

6. While in preview mode, the Brightness slider can be adjusted to brighten dark images.

7. The user may adjust the focus manually to enhance the appearance of the previewed image. Click the
Manual focus radio button on the Preview window, then use the left and right arrows (shown in the red
box, below) to adjust the focus settings.

Preview Window with Manual Focus

8. When satisfied with the previewed image, select Capture at the top of the software.

Optional Camera Setup (Image Acquisition)

If operating the camera in automatic mode is not sufficient for the application, try utilizing the manual mode
to gain additional camera control.

1. Access the camera and turn the camera dial to M, the next position on the dial. (The dial is preset
to A-DEP and will only turn in one direction.)

ChromaDoc-It Imaging System 8

2. A new camera window appears with additional options:

Camera Control in Manual Mode

3. Select the desired options for an enhanced image. See the camera manual for detailed descriptions of
options.

4. Select Capture at the top of the software.

ChromaDoc-It Imaging System 9

Replacement Parts and Accessories

The following replacement parts and accessories are available for use with the ChromaDoc-It Imaging
System:

Description Part Numbers

Replacement Tube, 15 watt, 254nm UV 34-0008-01 – 2 required
Replacement Tube, 15 watt, 365nm UV 34-0009-02 – 2 required
Replacement Tube, 4 watt, white light 34-0066-01 – 2 required

Doc-ItLS Analysis Software 97-0185-02

Thermal Printer, Digital (115/230V) 89-0069-06
Thermal Printer, Digital (230V) 89-0069-07
Thermal Printer, Digital (230V for Europe) 98-0069-15
Thermal Paper, 4 rolls (800 images) 89-0038-01
Thermal paper, 16 rolls (3200 images) 89-0038-04
Thermal Paper, 40 rolls (8000 images) 89-0038-05

Filter, 50mm, sq., SYBR Green and EGFP 38-0219-01 Filter, 50mm, sq., Ethidium Bromide 38-0220-01

ChromaDoc-It Imaging System 10

If residing in North America, South

If residing in Europe, Africa, the Middle

Call [phone] or (909) 946-

Call [phone], and ask for
E-mail your message to:

E-mail your message to: [e-mail]

Fax Customer Service, and send it to

Fax Customer Service, and send it to: +44(0)

Write to: UVP, LLC 2066 W. 11th

Write to: Ultra-Violet Products Ltd

Service Procedures

Return Procedure

A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service before
returning any product.

Technical Assistance

UVP offers technical support on all of its products. If there are questions about the product’s use, operation
or repair, please contact our offices at the locations below, or go to UVP’s web site and click the Tech
Support > BioImaging Systems.

Warranty

UVP's products are guaranteed to be free of defect s in materi al s, workmanship and manufacture for one (1)
year from date of purchase. Consumable and disposabl e parts including, but not li mited to tub es and filters, are
guaranteed to be free from defects in manufacture a nd materi als for ninet y (90) day s fr om da te of purchase.
Transilluminators carry a two (2) year war ranty fr om date of purchase. If equipment failure or malfunction
occurs during the warranty period, UVP shall examine the inoperative equipment and have the option of
repairing or replacing any part(s) which, in the judgment of U VP, were originally defective or became so under
conditions of normal usage and service.

No warranty shall apply to this instrument, or part thereof, t h at has been su bject to accident, negligence,
alteration, abuse or misuse by the end-user. Moreover, UVP makes no warranties whatsoever w ith r espect to
parts not supplied by UVP or that have been installe d, used an d/or ser v iced other than in strict compliance with
instructions appearing in this manual.

In no event shall UVP be responsible to the end-user for any incidental or consequential damages, whether
foreseeable or not, including but not limited to property damage, inability to use equipment, lost business,
lost profits, or inconvenience arising out of or connected with the use of instruments produced by UVP. Nor
is UVP liable or responsible for any personal injuries occurring as a result of the use, installation and/or
servicing of equipment.

America, East Asia or Australia:

3197, and ask for Customer Service

during regular business days, between
7:00 am and 5:00 pm, PST.

[e-mail]

[phone]

Street, Upland, CA 91786 USA

East of Western Asia:

Customer Service during regular business

days between 9:00 am and 5:30 pm.

1223-420561

Unit 1, Trinity Hall Farm Estate, Nuffield Road,

Cambridge CB4 1TG UK

This warranty does not supersede any statutory r ights that may be av ailable in certain countries.

Doc-It is a registered trademark of UVP, LLC. ChromaDoc-It is a trademark of UVP, LLC.