UVP ChromaDoc-It Imaging System User Manual

ChromaDoc-It™ Imaging System

Installation and User Instructions

UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]

Web Site: www.uvp.com

81-0295-01 Rev E

ChromaDoc-It Imaging System 2

ambient light

DigiCam 125 camera

with diopter

TLC Filter is located

Introduction

The ChromaDoc-It Imaging System is designed for capturing brilliant Thin Layer Chromatography (TLC) images.
The system combines:

 Viewing Cabinet with built-in epi white light and UV: 254nm (shortwave) and 365nm (longwave) UV
 DigiCam 125 digital color cam era
 Ethidium Bromide (EtBr) Filter
 Doc-It

Cap enclosure (not shown)

reduces external light

when taking pictures

®

LS Acquisition Software (refer to software manual for installation)

inside the camera

mount

Access curtain blo ck s

Overhead UV and white

light

Viewing Cabinet

The ChromaDoc-It Imaging System components include a Chromato-Vue viewing cabinet with epi 254nm and
365nm UV to fluoresce various stained gels. The cabinet provides white light for illuminating the cabinet interior.
For additional information about the cabinet, refer to the separate Chromato-Vue Cabinet manual.

UV Filter

The TLC, orange-colored UV blocking band pass filter, is installed inside the cabinet at the UVP factory. The filter
is used to absorb UV and IR radiation from the transilluminator and to enhance the orange/pink bands generated
by EtBr stained gels. The filter can be removed when imaging non-fluoresc ent med ia in order to produce brighter
images.

Camera and Diopter

The digital color camera includes a zoom lens. A close-up diopter is connected to the lens at UVP’s factory.

ChromaDoc-It Imaging System 3

Doc-ItLS Acquisition Software

Doc-ItLS Software

The Doc-ItLS software loads onto the user’s existing computer. The software controls the camera acquisition
functions as well as enhancement and reporting features. For information on installation and use of the software,
refer to the Doc-ItLS Software Manual (pdf file on the software CD).

Operating System Requirements

• Windows 7, Vista or XP SP2 or later

• Internet Explorer 6.0 or higher [To locate the version of Internet Explorer, open Internet Explorer and

click on Help->About Internet Explorer]

• Intel Pentium Processor or equivalent, 1.6 GHz or higher

• 1 GB of RAM or greater

• 200 MB of available hard disk space for the program, more for data

• CD-ROM drive

• One Universal Serial Bus (USB) for connecting the camera

• Color monitor, supporting at least 1024 x 768 resolution and 16-bit or better colors; 24-bit or 32-bit color

is strongly recommended

NOTE: For 21 CFR Part 11 support functionality, the computer partition must be formatted with NTFS. Refer to
the Doc-ItLS software manual for instru ctio ns.