UVP ChemiDoc-It 2 Imagers User Manual
GelDoc-It
2
®
& ChemiDoc-It
2
®
Installation and User Instructions
Imagers
UVP, LLC Ultra-Violet Products Ltd.
2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: (800) 452-6788 Phone: +44(0)1223-420022
Fax: (909) 946-3597 Fax: +44(0)1223-420561
Web Site: www.uvp.com
81-0220-01 Rev N
GelDoc-It
2
and ChemiDoc-It2 Imagers 2
Table of Contents
Table of Contents ......................................................................................................................................................... 2
Introduction .................................................................................................................................................................. 3
System Components ................................................................................................................................................... 4
Minimum Computer Requirements ............................................................................................................................ 4
Cameras .................................................................................................................................................................... 5
Optional Equipment ................................................................................................................................................... 6
Setup Instructions ........................................................................................................................................................ 8
Components .............................................................................................................................................................. 8
Connecting the Power Cables ................................................................................................................................... 8
Installing the Emission Filter ...................................................................................................................................... 8
Camera Setup ........................................................................................................................................................... 9
Software Installation ................................................................................................................................................ 12
Using the System ....................................................................................................................................................... 15
Powering Up the System ......................................................................................................................................... 15
Using the Filters ...................................................................................................................................................... 15
Using the Transilluminator ....................................................................................................................................... 16
Using the Overhead (Epi) Lighting .......................................................................................................................... 16
Using the UV Gel Viewer Window ........................................................................................................................... 16
Capturing Images and Using Templates .................................................................................................................. 17
Image Focusing ....................................................................................................................................................... 17
Capturing Images .................................................................................................................................................... 17
Using Templates ..................................................................................................................................................... 18
Service Procedures .................................................................................................................................................... 20
Return Procedure .................................................................................................................................................... 20
Replacement Parts and Accessories ....................................................................................................................... 20
Troubleshooting ....................................................................................................................................................... 20
Care and Cleaning .................................................................................................................................................. 21
Technical Support ................................................................................................................................................... 21
GelDoc-It
2
and ChemiDoc-It2 Imagers 3
Introduction
The GelDoc-It2 Imager is designed as a high resolution imager capable of capturi ng, doc u ment ing and
analyzing fluorescent gel images.
In addition to gel documentation capabilities, the ChemiDoc-It
grade CCD camera allowing users to capture, document and analyze chemiluminescent blot images,
including Western blots.
The GelDoc-It
for image acquisition and analysis function s. The darkroom has a UV-blocking gel viewer window, built-in
overhead epi white lighting, a UV transilluminator and a four-position emission filter wheel with an ethidium
bromide (EtBr) emission filter included as sta ndard .
2
Imager is equipped with a cooled, scientific-
2
and ChemiDoc-It2 Imagers incorporate a light-tight darkroom with VisionWorks®LS software
GelDoc-It
Processor:
Pentium class, 1.6 GHz or higher
RAM:
2 GB (4 GB Preferred)
Display:
1024 x 768
Operating System:
Microsoft Windows XP Service Pack 2 or Newer (32-bit or 64-bit)
Internet Browser:
Microsoft Internet Explorer 6.0 or later
Computer USB Ports:
Computer must come equipped with a minimum of three USB
require additional USB ports.
Camera and Lens Kit
Lighting and UV
Darkroom
Access Door
Epi Overhead
White Light
Four Position
UV Safety
Interlock Switch
UV-Safe Gel
Window
Roll-Out
Transilluminator
LED White Light
Plate (Optional)
Access port
2
and ChemiDoc-It2 Imagers 4
System Components
Refer to the packing slip and pictured components for parts included with the system.
Emission Filter
Selector
for optional
BioLite™
MultiSpectral
Light Source
(actual configuration may
be different than shown in
the photo)
Timer Switches
Viewer
Minimum Computer Requirements
ports; additional peripherals (mouse, keyboard, printer, etc.) may
GelDoc-It
2
and ChemiDoc-It2 Imagers 5
Cameras
Lens Kit (Motorized or Manual)
Ethidium Bromide (EtBr) Emission Filter
Darkroom
GelDoc-It2: The GelDoc-It2 is equipped with the GelCam 310 camera, a scientific-grade monochrome
CCD camera with a resolution of 2.0MP (1600x1200) with USB 2.0 PC interface.
ChemiDoc-It
MegaCam 810 scientific-grade monochrome CCD camera. All cameras are Peltier cooled and offer full
16-bit file bit depth:
The BioChemi 510 camera has 2.1MP resolution with Peltier cooling to -35°C from ambient. The
BioChemi 510 has a peak quantum efficiency of 50% and is capable of binning from 1x1 to 8x8.
The OptiChemi 610 camera has 3.2MP (2184x1472) resolution with Peltier cooling to -50°C from
ambient. The OptiChemi 610 has a peak quantum efficiency of 86% and is capable of binning from
1x1 to 10x10.
The MegaCam 810 camera has 8.1 MP (3296x2472) resolution with Peltier cooling to -35°C from
ambient. The MegaCam 810 has a peak quantum efficiency of 50% and is also capable of binning
from 1x1 to 8x8.
All camera settings are factory pre-set for optimum performance when viewing gels, films or
membranes under low light level conditions. Contact UVP Technical Support prior to making any
adjustments to the camera settings.
2
: The ChemiDoc-It2 is equipped with the BioChemi™ 510, OptiChemi™ 610 or
The zoom lens used with the GelCam 310 and BioChemi 510 cameras comes in two configurations:
The motorized lens is controlled via VisionWorksLS Software.
The manual lens requires manual user adjustment.
Some lenses may be fitted with a close-up diopter. The diopter is used for focusing on obje cts at the
focal length of the GelDoc-It
The ethidium bromide (50mm square) UV-blocking bandpass interference filter blocks UV and IR
radiation emitted from the transilluminator. T he filter is pla ce d in the filter wheel below the camera
assembly. The filter allows visualization of fluorophores from 580-630nm, targeting the ethidium
bromide emission peak of 605nm.
Additional filters are available for other specific fluorophores. Filters can also be removed when imaging
non-fluorescent media (including chemiluminescent blots, protein gels, colony plates, etc.) in order to
produce brighter images. Contact UVP for ordering information.
The darkroom is light tight to provide optimal imaging conditions. Darkroom features include:
2
/ChemiDoc-It2 darkroom.
Overhead white light
Roll-out transilluminator tray
UV-safe gel viewer window built into the darkroom door
Side pocket for storage of materials
Four position emission filter wheel
Brackets for installing optional UV handheld lamps for epi (overhead) UV illumination
Built-in power ports for transilluminator and optional overhead UV handheld lamps
Built-in power cord for optional LED White Light Plate
UV safety interlock switch with manual override to disable UV transillumination and
optional UV epi illumination when darkroom door is opened
GelDoc-It
UV Lamp
Fluorescent Focus Target
2
and ChemiDoc-It2 Imagers 6
Transilluminator
The GelDoc-It2 and ChemiDoc-It2 Imagers can accommodate UVP’s Benchtop and FirstLight
transilluminator models. UVP offers a variety of transilluminator configurations, including Benchtop
models with multiple wavelengths and variable intensities as well as the highly uniform, patented
FirstLight
Note: For UV protection and to extend the life of the UV transilluminator, the system incorporates a tenminute transilluminator shutoff timer. The timer shuts off the transilluminator after ten minutes of
operation. A push button on the front of the darkroom allows the user to reset the timer. Alternately, the
timer can also be reset by opening and closing the darkroom door.
®
transilluminator. Refer to the packing slip for the transilluminator included with your system.
Fluorescent Focus Target
The UVP Fluorescent Focus Target fluoresces when placed
on a UV transilluminator or when exposed to overhead UV.
The Target provides sharp fluorescent images to aid in
adjusting the lens and camera settings for ideal imaging
results.
Optional Equipment
UVP offers a variety of optional equipment to support the needs of varying laboratory environments. Refer to
“Replacement Parts and Accessories” at the end of this manual for optional equipment part numbers.
Thermal Printer
The thermal printer provides archive quality, 256 grayscale
prints and five optional cost-effective print size s.
Thermal Printer
UV Handheld Lamps for Overhead Lighting
Two 4-watt ultraviolet lamps can be connected inside the
darkroom to provide epi UV illumination. These lamps can be
switched on or off using the switch on the front of the
darkroom and can also be removed from the darkroom and
used as standalone handheld lamps (as seen in the image to
the right).
GelDoc-It
LED White Light Plate
2
and ChemiDoc-It2 Imagers 7
LED White Light Plate
The LED White Light Plate emits high uniformity with less
than 5% coefficient of variance (CV). Plug the LED White
Light Plate directly into the power supply within the system
darkroom, or store it in the exterior darkroom side pocket
when not in use.
Converter Plates
An alternate to the LED White Light Plate, the UV/White
Converter Plate allows imaging of non-fluorescent sta ined
media with an ultraviolet transilluminator. The converter plate
is specially coated to convert 302nm UV output to white light
rather than using a separate white light box or plate.
The Visi-Blue™ Converter Plate (not shown) converts UV
to a safe 460-470nm blue wavelength designed for use with
blue excitation samples and SYBR Green, SYPRO Orange
and GFP stains.
UV/White Converter Plate
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