UVP GelDoc-It2, ChemiDoc-It2 Installation And User Instructions Manual

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GelDoc-It

®

2

& ChemiDoc-It

®

2

Imagers

Installation and User Instructions

UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]

Web Site: www.uvp.com

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Table of Contents

1

Basic information ............................................................................................... 4

1.1

User manual notes .................................................................................... 4

1.2

Intended use .............................................................................................. 4

2

Technical data .................................................................................................... 5

2.1

Specifications ............................................................................................ 5

2.2

Minimum computer requirements .............................................................. 5

2.3

Cameras and lenses .................................................................................. 5

3

Safety instructions ............................................................................................. 7

3.1

General notes ............................................................................................ 7

3.2

Safety Instructions – Operation and Maintenance .................................... 8

4

System design .................................................................................................... 9

4.1

Components .............................................................................................. 9

4.2

Camera Models ....................................................................................... 10

4.3

Ethidium Bromide (EtBr) emission filter ................................................... 10

4.4

Transilluminator ....................................................................................... 10

4.5

Fluorescent Focus Target ........................................................................ 10

4.6

Optional Equipment ................................................................................. 11

4.7

Thermal Printer ........................................................................................ 11

4.8

UV Handheld Lamps for Epi (Overhead) Lighting ................................... 11

4.9

LED White Light Plate ............................................................................. 11

4.10

Converter Plates ...................................................................................... 11

4.11

VisionWorks

®

Acquisition and Analysis Software ................................... 12

5

Set-Up ............................................................................................................... 13

5.1

Scope of supply ....................................................................................... 13

5.2

Connecting the power cables .................................................................. 13

5.3

Installing emission filters .......................................................................... 14

5.4

Camera Setup.......................................................................................... 14

6

Software Installation ........................................................................................ 20

6.1

Installing VisionWorks Software............................................................... 20

6.2

Registering the Software ......................................................................... 20

6.3

Installing Camera Drivers ........................................................................ 21

7

Using the imaging system .............................................................................. 22

7.1

Powering up the system .......................................................................... 22

7.2

Using the Filters ....................................................................................... 23

7.3

Using the Transilluminator ....................................................................... 23

7.4

Using the Overhead (Epi) Lighting .......................................................... 23

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7.5

Using the UV Gel Viewer Window ........................................................... 24

8

Capturing Images and Using Templates ....................................................... 25

8.1

Image Focusing ....................................................................................... 25

8.2

Capturing Images .................................................................................... 26

8.3

Using Templates ...................................................................................... 27

9

Maintenance, replacement parts/accessories .............................................. 29

9.1

Care and cleaning ................................................................................... 29

9.2

Replacement parts and accessories ....................................................... 29

9.3

Troubleshooting ....................................................................................... 32

10

Disposal ............................................................................................................ 32

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Basic information

1.1

User manual notes

The Imagers are intended for operation by qualified specialist personnel observing this user
manual.

The user manual informs about the design and function of the Imagers and provides the
necessary know-how for the safe handling of the device and its components to personnel
familiar with analysis. The user manual further includes notes on the maintenance and
service of the equipment.

User manual conventions
Instructions for action which occur in chronological order are numbered and combined into
action units and furnished with the corresponding results.

Lists which are not in chronological order are shown as itemized lists, sub-listings as bullet
points.

Safety notes are indicated by pictographs and signal words. The type and source of the
danger are stated together with notes on preventing the danger. The meaning of the
pictographs and signal words used is explained in the chapter "Safety instructions".

1.2

Intended use

The GelDoc-It2 Imager is a high resolution imager, capable of capturing, documenting and
analyzing fluorescent gel images.

The ChemiDoc-It2 Imager is designed as a high resolution imager capable of capturing,
documenting and analyzing fluorescent gel images, as well chemiluminescent blot images,
including Western blots. The system is equipped with a cooled, scientific-grade CCD
camera.

Both imagers incorporate a light-tight darkroom with VisionWorks® software for image
acquisition and analysis functions. The darkroom has a UV-blocking gel viewer window,
built-in overhead epi white lighting, a UV transilluminator and a four-position emission filter
wheel with an ethidium bromide (EtBr) emission filter included as standard.

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Technical data

2.1

Specifications

Power Requirements

100/115V, 50/60Hz; 2.1 Amps at 120 Volts
230V, 50/60Hz; 1.1 Amps at 230 Volts
Mains supply voltage fluctuations are not to exceed 10 percent of

the nominal supply voltage

Pollution Degree

2

Installation Category

II

Altitude

Up to 2000 m

Ambient Temperature

5 °C to 40 °C

Humidity

Maximum relative humidity of 80 % for temperatures up to 31 °C,
decreasing linearly to 50 % maximum relative humidity at 40 °C

2.2

Minimum computer requirements

 Microsoft Windows 10, 8, 7 or XP (SP2), 32-bit or 64-bit
 Microsoft Internet Explorer 6.0 or later
 Pentium class processor or equivalent, 1.6 GHz or higher
 2 GB of RAM (4 GB preferred)
 200 MB of available hard disk space for the program, more for data
 CD-ROM drive
 USB ports: The computer must come equipped with a minimum of three USB2.0

ports. Additional peripherals (mouse, keyboard, printer, etc.) may require additional
USB ports.

 Color monitor, supporting at least 1024 x 768 resolution and 16-bit or better; 24-bit or

32-bit color is strongly recommended

IMPORTANT

For 21 CFR Part 11 support functionality, the computer partition must be formatted
with NTFS. Refer to the VisionWorks software manual for instructions.

2.3

Cameras and lenses

The GelDoc-It2 Imager is equipped with the GelCam 315 camera, a monochrome
CMOS camera with a resolution of 5.0MP (2592 x 1944) with USB 3.0 PC interface.

The ChemiDoc-It2 Imager can be equipped with two different cameras.

Camera with Peltier cooling (for gel documentation and chemiluminescence
documentation):

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Available are: CCD™ 515 or 815 CCD scientific-grade monochrome CCD camera. All
cameras are Peltier cooled and offer full 16-bit file bit depth:

The 515 CCD camera has 2.1 MP resolution with Peltier cooling to - 57 °C from ambient.
The 515 CCD has a peak quantum efficiency of 50 % and is capable of binning from 1 x 1
to 8 x 8.

The 815 CCD camera has 8.1 MP (3296 x 2472) resolution with Peltier cooling to -57
°C from ambient. The 815 CCD has a peak quantum efficiency of 50 % and is also
capable of binning from 1 x 1 to 8 x 8.

All camera settings are factory pre-set for optimum performance when viewing gels, films or
membranes under low light level conditions. Contact Technical Support prior to making any
adjustments to the camera settings.

Lens Kit (Motorized or Manual)

The lens used comes in two configurations:

 The motorized lens is controlled via VisionWorks Software.
 The manual lens requires manual user adjustment.

Some lenses may be fitted with a close-up diopter. The diopter is used for focusing on
objects at the focal length of the darkroom.

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Safety instructions

3.1

General notes

For your own safety, please read this chapter carefully before operating. Observe all safety
notes listed in this user manual.

Symbols and signal words used

The user manual uses the following symbols and signal words to indicate safety hazards,
warnings and instructions.

General warning

Caution: ultraviolet radiation

Important: hints or useful information

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3.2

Safety Instructions – Operation and Maintenance

The system is designed for function, reliability, and safety. The unit may include shortwave
UV, which is a powerful source of UV radiation that will cause damage to unprotected eyes
and skin.

For your own safety observe the following notes:

 The device must only be operated if all protective equipment is present, and the system

is properly installed and fully functional.

 Free access to the power plug and main power switch on the back of the unit must be

ensured.

 Disconnect the power supply before servicing the system
 For UV protection and to extend the life of the UV transilluminator, the system

incorporates a customizable transilluminator shutoff timer built into the software. For
additional information, refer to Section 4.4.

Caution: Do not override the UV safety switch located on the darkroom without the use of
protective equipment and face shields.

Caution: Shortwave UV (254 nm) is a powerful source of UV radiation that will cause
damage to unprotected eyes and skin. Before operating any unit, ensure all personnel in
the area are properly protected and that instructions for use of this equipment are followed.

System design

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System design

4.1

Components

Refer to the packing slip and pictured components for specific parts and components
included with the system.

Note: The delivered system may look slightly different (without cover, only with different
camera bracket).

GelDoc-It®2 & ChemiDoc-It®2 Imagers

Camera and Lens Kit

(actual configuration may

be different than shown in

the photo)

Lighting and UV

Timer Switches

Epi Overhead

White Light

Four Position

Emission Filter

Selector

UV Safety

Interlock Switch

UV-Safe Gel

Viewer

Window

Roll-Out

Transilluminator

LED White Light

Plate (Optional)

Access port

for optional

BioLite™

MultiSpectral

Light Source

System design

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4.2

Camera Models

815 CCD and 515 CCD Cameras

Specifications

815 CCD

515 CCD

Type

CCD, Monochrome

CCD, Monochrome

Resolution

3296 x 2472, 8.1 MP

2.1 MP

Bit Depth

16 bit

16 bit

Quantum Efficiency

50 % peak

50 % peak

Cooling

- 57 °C from ambient

- 57 °C from ambient

Binning

Yes, 1 x 1 through 8 x 8

Yes, 1 x 1 through 8 x 8

PC Interface

USB2.0

USB2.0

4.3

Ethidium Bromide (EtBr) emission filter

The ethidium bromide (50 mm2) UV-blocking bandpass interference filter blocks UV and IR
radiation emitted from the transilluminator. The filter is placed in the slide-out filter wheel
assembly on the side of the Imager. The filter allows visualization of fluorophores from 580 ­630 nm, targeting the ethidium bromide emission peak of 605 nm.

Additional filters are available for other specific fluorophores. Filters can also be removed when
imaging non-fluorescent media (including chemiluminescent blots, protein gels, colony plates,
etc.) in order to produce brighter images.

4.4

Transilluminator

The GelDoc-It2 and ChemiDoc-It2 Imagers can accommodate UVP’s Benchtop and FirstLight
transilluminator models. UVP offers a variety of transilluminator configurations, including
Benchtop models with multiple wavelengths and variable intensities as well as the highly
uniform, patented FirstLight® transilluminator. Refer to the packing slip for the transilluminator
included with your system.

Note: For UV protection and to extend the life of the UV transilluminator, the system
incorporates a ten-minute transilluminator shutoff timer. The timer shuts off the
transilluminator after ten minutes of operation. A push button on the front of the
darkroom allows the user to reset the timer. Alternately, the timer can also be reset by
opening and closing the darkroom door.

4.5

Fluorescent Focus Target

The Fluorescent Focus Target fluoresces when placed
on a UV transilluminator or when exposed to overhead
UV. The Target provides sharp fluorescent images to
aid in adjusting the lens and camera settings for ideal
imaging results.