UVP BioSpectrum Imaging System User Manual

Ultra-Violet Products Ltd.

BioSpectrum® Imaging System™

Instruction Guide

______________________________________________________________________________

UVP, LLC

2066 W. 11th Street, Upland, CA 91786
Tel: [phone]
Fax: [phone]

Web Site: www.uvp.com

Tel: [phone] Fax: [phone]

Nuffield Road, Cambridge CB4 1TG UK

Unit 1, Trinity Hall Farm Estate

81-0346-01 Rev F

BioSpectrum Imaging System 2

Table of Contents

Introduction .................................................................................................................................................................. 3

Components ................................................................................................................................................................. 4

Darkroom Cabinet ..................................................................................................................................................... 5

Five Position Filter Wheel .......................................................................................................................................... 5

CCD Camera and Lens ............................................................................................................................................. 5

UV-Safe Gel Viewer Window..................................................................................................................................... 5

Lift Platform ............................................................................................................................................................... 5

Epi Illumination .......................................................................................................................................................... 6

LED White Light Illuminator ....................................................................................................................................... 6

Chemi Tray ................................................................................................................................................................ 6

UV Transilluminator ................................................................................................................................................... 6

VisionWorks

BioLite Access Ports ................................................................................................................................................. 7

Installation .................................................................................................................................................................... 8

Installing VisionWorksLS Software ............................................................................................................................ 8

Registering the Software ........................................................................................................................................... 8

Hardware Installation ................................................................................................................................................. 9

Camera Installation .................................................................................................................................................... 13

GelCam 310 Camera .............................................................................................................................................. 13

MegaCam 810, OptiChemi 610 and BioChemi 510 Cameras ................................................................................. 15

Operation .................................................................................................................................................................... 17

Using the System .................................................................................................................................................... 17

Capturing Images ....................................................................................................................................................... 20

Focusing the Lens Using Image Preview ................................................................................................................ 20

Capturing Images .................................................................................................................................................... 20

Service Procedures .................................................................................................................................................... 21

Return Procedure .................................................................................................................................................... 21

Troubleshooting ....................................................................................................................................................... 21

Replacement Parts and Accessories ....................................................................................................................... 21

Technical Support ................................................................................................................................................... 22

®

LS Software ......................................................................................................................................... 7

BioSpectrum Imaging System 3

Introduction

The BioSpectrum Imaging System is designed to automate research with one-touch preset or user-defined PC
controls for accurate, repeata b le imag ing and analy s is of che milu mines cen ce, fluorescence, bioluminescence and
colorimetric samples (dep ending upon configuration). The BioSpectrum incorporates a light-tight darkroo m with
VisionWorksLS software for automated control.

The darkroom has a UV-blocking window, built-in overhead 365nm UV, Visi-Blue™ 480nm and white light, UV
transilluminator, LED White Light Illuminator , chemiluminescence tray and five-position emission filter wheel with
three emission filters included. The Automated BioSpectrum includes a software-controlled lift platform, while the
Manual BioSpectrum includes a manually adjustable lift platform.

The BioSpectrum includes a highly sensitive CCD camera which supplies real-time, live preview images.

Automated and Manual BioSpectrum Imaging Systems

BioSpectrum Imaging System 4

CCD camera and lens are

Five position filter wheel with

and SYBR Gold filters included

Epi illumination with white light,

Access ports for optional

Gel viewer

door closed

Light tight

LED White Light Illuminator

UV transilluminator

roll-out tray

Height-adjustable lift

motorized)

Chemi tray (not shown)

VisionWorksLS software

Power indicator lights

Components

The BioSpectrum Imaging System is comprised of the following equipment:

 Darkroom Cabinet
 Automated Filter Wheel and Emission Filters (3 Included with System)
 CCD Camera and Lens:

o MegaCam 810 with either 50mm f/1.2 Fixed, 30mm f/1.4 Fixed, or f/2.8 Zoom Lens
o OptiChemi™ 610 with either 50mm f/1.2 Fixed, 30mm f/1.4 Fixed, or f/2.8 Zoom Lens
o BioChemi™ 510 with 12.5-75mm f/1.2 Zoom Lens
o GelCam 310 with 12.5-75mm f/1.2 Zoom Lens

 UV-Blocking Gel Viewer Window in Darkroom Door
 Automated or Manual Lift Platform (depending upon configuration)
 Epi Illumination
 Chemi Tray
 LED White Light Illuminator
 UV Transilluminator
 VisionWorks

Refer to the packing slip for a complete equipment list. System components may vary. The system may
additionally include a computer and monitor, printer or BioLite™ MultiSpectral Light Source.

®

LS Software

housed under the camera cover

BioLite MultiSpectral Light

Source

window blocks
UV and allows

sample viewing

with darkroom

darkroom with

wide access
door and UV
safety switch

Ethidium Bromide, SYBR Green

480nm and 365nm UV

platform (manual or

software-controlled

controls darkroom functions,

image capture and analysis

BioSpectrum Imaging System 5

Darkroom Cabinet

The BioSpectrum Imaging System is constructed of aluminum and is
fabricated to provide a light-tight chamber.

Darkroom Dimensions: 17.5W x 17.5D x 32H inches (445 x 445 x 813
mm)

Note: The camera cover will add additional height which will vary based
on camera application.

Five Position Filter Wheel

The filter wheel accommodates up to five emission filters. The system
comes standard with Ethidium Bromide, SYBR Green and SYBR Gold
filters. Software controls the selection of filters.

CCD Camera and Lens

The high sensitivity CCD camera and fast optics, housed in the top of
darkroom, generate high resolution images. The camera and motorized
lens are controlled by VisionWorksLS software.

Refer to the packing slip for the camera configured with your system
(Note: camera shown to the right may appear different).

Refer to the Cameras section of this manual for installation and
operation instructions for the camera shipped with your system.

UV-Safe Gel Viewer Window

The viewer in the darkroom door opens for UV-safe viewing of samples.
The acrylic window allows viewing without exposure to ultraviolet
radiation.

Lift Platform

The motorized lift platform in the Automated BioSpectrum, controlled
by the software, can be finely adjusted within a 10-inch range.

The manual lift platform in the Manual BioSpectrum can be manually
adjusted to six positions within the travel range.

BioSpectrum Imaging System 6

Epi Illumination

The LED White Light Illuminator emits high uniformity with less than 5%

The chemi tray creates a dark background for placement of

The system may be configured with a UV transilluminator to fluoresce

Standard overhead lighting includes 365nm UV, Visi-Blue 480nm and
white light. Lighting is controlled via the software interface.

NOTE: The BioSpectrum includes an interlock switch that turns off all
UV illumination, including the epi 365nm UV, when the darkroom door is
opened. Be sure the dar kroo m door is comple tely clos ed f or pr oper UV
operation.

LED White Light Illuminator

coefficient of variance (CV). Store the White Light Illuminator in the
exterior darkroom side pocket when not in use.

Chemi Tray

bioluminescent or chemilumin esc ent samples such as Western blots,
shown here placed on top of the FirstLight

UV Transilluminator

samples. The transilluminator is positioned on a roll out tray for easy
access.

NOTE: The BioSpectrum includes an interlock switch that turns off all
UV illumination, including the transilluminator, when the darkroom door
is opened. Be sure the darkroom door is completely closed for proper
UV operation.

®

UV Illuminator.

BioSpectrum Imaging System 7

VisionWorks®LS Software

BioLite Access Ports

VisionWorksLS software controls the darkroom functions and lighting as
well as the motorized lens and camera. The system may be configure d
with VisionWorksLS analysis software for analysis of gels, plates, blots
and other samples.

Additional optional equipment can be added to the BioSpectrum Imaging
System through the side access ports.

 Side access ports allow for connecting the external BioLite

MultiSpectral Light Source for directed lighting ranging from visible
to near IR (note: the Automated BioSpectrum has two access
ports while the Manual BioSpectrum has one access port).

 The access panel on the back of the darkroom provides access for

connecting additional equipment within the darkroom.

Minimum Computer Requirements

Processor: Pentium 4, 1.6 GHz or higher
RAM: 2GB (4GB Preferred)
Display: 1024 x 768
Operating System: Microsoft Windows XP Service Pack 2 or Newer (32-bit or 64-bit)
Internet Browser: Microsoft Internet Explorer 6.0 or later
Computer USB Ports: Computer must come equipped with a minimum of three USB ports; additional

peripherals (mouse, keyboard, printer, etc.) may require additional USB ports.