UVP BioDoc-It System User Manual

BioDoc-It

® Imaging System

Installation and User Instructions

UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]

Web Site: www.uvp.com

81-0301-01 Rev H

BioDoc-It Imaging System 2

System Introduction

BioDoc-It® Imaging System

The BioDoc-It Imaging System enables simple documentation of fluorescent and non-fluorescent gels,
membranes, blots, film plates and assays with the ability to save images to a USB storage device for later
quantitative analysis or enhancement for publication.

Images are saved in 8-bit TIFF or JPEG formats, allowing saved images to be read by most PC or MAC
programs. The BioDoc-It Imaging System is a cost effective solution for capturing quality images in a
compact standalone pack age, as no external computer is required.

The BioDoc-It comes in two configurations:

 BioDoc-It 220 Imaging System includes a high resolution, 1.3 megapixel camera, 8-48mm zoom

lens and side access doors.

 BioDoc-It 210 Imaging System includes a 0.3 megapixel camera and an 8-48mm zoom lens.

BioDoc-It Imaging System 3

8” LCD Touch Screen

White Light and Darkroom
Power Switches

Wide Access
Door

USB Drive

Filter Tray (located under the
Main System Power Button

UV Gel Viewer
Window

Transilluminator

FluorCam 210 or 220

UV to White Light Converter

System Components

BioDoc-It Components

Refer to the packing slip and pictured components for parts included with the system.

with Tilt Capability

Camera and Zoom Lens

camera) with EtBr Filter

Plate (order separately)

Side Access Doors (on
BioDoc-It 220 models)

BioDoc-It Imaging System 4

Camera

BioDoc-It 220 Models:

settings.

Lens

The BioDoc-It is equipped with an 8-48mm zoom

length of the BioDoc-It Imaging System’s darkroom.

Built-In Computer



Ethidium Bromide Filter (EtBr)

The Ethidium Bromide (EtBr) filter (50mm sq) UV

System Specifications

The BioDoc-It 220 is equipped with a FluorCam 220
Camera (resolution of 1.3MP).

BioDoc-It 210 Models:

The BioDoc-It 210 is equipped with a Fluor C am 210
Camera (resolution of 0.3MP).

Both the FluorCam 220 and FluorCam 210 use a
USB 2.0 PC connection. All camera settings are
factory pre-set for optimum performance when
viewing gels, films, or membranes under low light
level conditions. Contact UV P T echnic al Suppor t
before making any adjustments to the camera

lens fitted with a close-up diopter and step-up ring,
allowing the camera to be held in a fixed position.
The diopter is for focusing on objects at the focus

Internal Storage: 60GB or more

 USB 2.0 Ports: 1 front, 4 in the back
 USB Storage Device Capacity: 1GB (minimum)
 Software: TS software

blocking band pass interference filter blocks UV and
IR radiation emitted from the transilluminator. The
filter is placed in the filter tray below the camera/lens
assembly.

The filter allows visualization of fluorophores from
580-630nm, targeting the EtBr emission peak of
605nm.

The EtBr filter can be substituted for other specific
fluorophore filters or removed when imaging non­fluorescent media (protein gels, colony plates, etc.)
in order to produce brighter images.

BioDoc-It Imaging System 5

Darkroom

Darkroom features include:

 UV gel viewer window
 Overhead white light
 Wide access door with UV safety interlock switch

Transilluminator

A transilluminator is included w ith the BioDoc-It system. UVP offers a variety of transilluminators, from benchtop
models with 8-watt, single wav eleng th and sin gle inte nsi ty to models with multiple wavelengths and high/low
intensities. UVP also offers the high-end FirstLight®, a highly uniform, 302nm UV transilluminator.

LCD Touch Screen

The display is an 8-inch VGA color touch screen with tilt adjustment connected to the darkroom cabinet. The
touch screen allows the user to preview, snap, save and print images, as well as select certain preference
options, without the need to use an external mouse or keyboard. However, should the user desire, a mouse
and/or keyboard (not included) can be used with the BioDoc-It.

A stylus pen is included for increased pointer control for selecting software options on the touch screen.

UVP Focus Target

The UVP Focus Target fluoresces when placed on a transilluminator
or when exposed to overhead UV. The target provides sharp,
fluorescent images to aid in adjusting the focus of the camera.

Removable USB Stick

The removable USB stick, included with the system, has 8GB
memory (minimum) and connects to the system to allow for saving
and transferring of images.

UVP Focus Target

BioDoc-It Imaging System 6

UV/White Converter Plate

Optional Equipment

Refer to the Replacement Parts and Accessories section of this manual for optional equipment part numbers.

Thermal Printer

The Thermal Printer provides archive quality, 256 grayscale prints
and five optional cost-effective print sizes.

Converter Plates

The UV/White Converter Plate allows imaging of non-fluorescent
stained media with an ultraviolet transilluminator. The converter plate
is specially coated to convert 302nm UV output to white light.

The Visi-Blue™ Converter Plate (not shown) converts UV to 460­470nm and is designed for use with blue excitation samples such as
SYBR Green, SYPRO Orange and GFP stains.

Thermal Printer