UVP biodoc-it 2 Installation And User Instructions Manual

BioDoc-It®2 Imaging System

Installation and User Instructions

UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]

Web Site: www.uvp.com

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Table of Contents

Table of Contents................................................................................................................................................... 2

Introduction ........................................................................................................................................................... 3

System Components .............................................................................................................................................. 4

Specifications ............................................................................................................................................................ 4

Built-In Touch Screen Computer ................................................................................................................................ 5

Cameras and Lenses .................................................................................................................................................. 5

Ethidium Bromide (EtBr) Emission Filter ................................................................................................................... 5

Darkroom .................................................................................................................................................................. 5

Transilluminator ........................................................................................................................................................ 6

LCD Touch Screen ...................................................................................................................................................... 6

VisionWorks touch Software ..................................................................................................................................... 6

Fluorescent Focus Target .......................................................................................................................................... 6

USB Flash Drive ......................................................................................................................................................... 6

Optional Equipment .................................................................................................................................................. 6

Setup Instructions .................................................................................................................................................. 7

Components .............................................................................................................................................................. 7

Connecting the Power Cables ................................................................................................................................... 7

Installing Emission Filters .......................................................................................................................................... 7

Camera Setup and Installation .................................................................................................................................. 7

The camera and zoom lens are assembled at the UVP factory. ................................................................................ 7

Note: The zoom lens shipped may appear different than pictured. .......................................................................... 7

Using the System ................................................................................................................................................... 8

Powering Up the Tablet ............................................................................................................................................ 9

Operating the VisionWorks touch Interface .............................................................................................................. 9

Using the Transilluminator ....................................................................................................................................... 9

Using the Epi (Overhead) White Light ..................................................................................................................... 10

Using the UV Gel Viewer Window ........................................................................................................................... 10

Image Focusing ....................................................................................................................................................... 10

Image Zooming ....................................................................................................................................................... 10

Touch Screen Interface ........................................................................................................................................ 11

Setting User Preferences ......................................................................................................................................... 11

Identifying the Touch Screen Buttons and Functions .............................................................................................. 13

Using Templates ..................................................................................................................................................... 18

Connecting to a Network ........................................................................................................................................ 19

Installing Drivers or Additional Software ................................................................................................................ 19

Service Procedures .............................................................................................................................................. 20

Return Procedure .................................................................................................................................................... 20

Replacement Parts and Accessories ........................................................................................................................ 20

Troubleshooting ...................................................................................................................................................... 21

Replacing Transilluminator Bulbs ........................................................................................................................... 21

Follow these steps to replace the UV tubes in the system’s transilluminator: ........................................................ 21

Technical Support .................................................................................................................................................... 22

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Introduction

The BioDoc-It2 Imaging System enables simple documentation of fluorescent and non-fluorescent gels,
film plates and assays with the ability to save images to a USB flash drive or network location for later
quantitative analysis or enhancement for publication.

Images are saved in a variety of selectable formats, allowing saved images to be read by most PC or Mac
programs. The BioDoc-It2 Imaging System is a cost effective solution for capturing quality images in a
compact standalone package, as no external computer is required.

The BioDoc-It2 comes standard with the GelCam 315 camera, which includes a 5.0 MP (2592 x 1944)
resolution, an 8-48mm f/1.2 zoom lens, and 16-bit file bit depth. Multiple transillumination options are
available, including single UV (302nm), 2UV™ (302/365nm) and 3UV™ (302/365/254nm) versions with
illumination areas ranging from 20 x 20cm to 25 x 26cm

*System/software configurations may vary by country. Contact UVP or authorized distributor for details.

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10” Integrated Touch

Screen

White Light and UV
Power Buttons

Wide Access
Door

USB Port (Not Shown)

Two-Position Filter Tray,
Including EtBr Filter

Tablet power switch

UV Gel Viewer
Window

GelCam 315 Camera and
Manual Zoom Lens

Transilluminator
Controls

Side Access Door

System Components

Refer to the packing slip and pictured components below for specific parts and components included with
the system.

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Size

10.1“ touch screen

Screen

Multi-touch, 1280 x 800 Resolution

Operating System

Windows® 10 Home (32 bit)

Hard Drive Capacity

64 GB Flash Storage

Memory

2 GB RAM

Wireless Networking Capability

802.11 b/g/n Wi-Fi and Bluetooth 4.0

Connectivity Ports

1 USB (side of system)
6 USB (rear of system)

Software

VisionWorks touch Software

Specifications

Power Requirements: 100/115V, 50/60Hz; 3.1 Amps at 120 Volts
230V, 50/60Hz; 1.55 Amps at 230 Volts
Mains supply voltage fluctuations are not to exceed 10 percent of the nominal

supply voltage

Pollution Degree: 2
Installation Category: II
Altitude: Up to 2000m
Ambient Temperature: 5°C to 40°C
Humidity: Maximum relative humidity of 80% for temperatures up to 31°C, decreasing

linearly to 50% maximum relative humidity at 40°C

Built-In Touch Screen Computer

Cameras and Lenses

The BioDoc-It2 315 is equipped with the GelCam 315 Camera, with a resolution of 5.0MP. The system is
equipped with an 8-48mm zoom lens, allowing the user to zoom in and out on the sample within the
darkroom.

The GelCam 315 uses a USB 2.0 connection. All camera settings are factory pre-set for optimum
performance when viewing gels and films under low light level conditions. Contact UVP Technical Support
before making any adjustments to camera settings.

Ethidium Bromide (EtBr) Emission Filter

The ethidium bromide (50mm2) UV-blocking bandpass interference filter blocks UV and IR radiation emitted
from the transilluminator. The filter is placed in the two-position emission filter assembly and allows
visualization of fluorophores from 580-630nm, targeting the ethidium bromide emission peak of 605nm.

Additional filters are available for other specific fluorophores, including custom filters. Filters can also be
removed when imaging non-fluorescent media (including protein gels, colony plates, etc.) in order to
produce brighter images. Contact UVP for ordering information.

Darkroom

Darkroom features include:

 Integrated 10” touchscreen interface
 Integrated transilluminator and epi (overhead) LED white lights
 UV-safe gel viewer window built into the darkroom door
 Side doors for easy access to the interior of the darkroom (for placement or excision of samples)
 Two-position emission filter selector
 UV safety interlock switch to disable UV transillumination when the main darkroom door is opened

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Fluorescent Focus Target

UV/White Converter Plate

Transilluminator

The BioDoc-It2 Imaging System includes an integrated UVP Benchtop transilluminator. UVP offers a variety
of transilluminator configurations, including models with multiple wavelengths and variable intensities.

LCD Touch Screen

The BioDoc-It2 Imaging System contains a fully integrated 10.1-inch color touchscreen computer. The touch
screen allows the user to perform a variety of tasks, including previewing, capturing, saving and printing
images, as well as selecting preference options, without the use of an external mouse or keyboard.

For users who prefer not to use the touch screen interface, an external keyboard and mouse can be used
via any available system USB ports.

VisionWorks touch Software

Image acquisition functions for the BioDoc-It2 are controlled using the VisionWorks touch software interface.
Software features include image preview, capture and save functions.

For more information, see the Touch Screen Interface section in this manual.

Fluorescent Focus Target

The UVP Fluorescent Focus Target fluoresces when placed on a
UV transilluminator or when exposed to overhead UV. The Target
provides sharp fluorescent images to aid in adjusting the lens and
camera settings for ideal imaging results.

USB Flash Drive

The removable USB flash drive, included with the system, has 8GB memory (minimum) and connects to the
system to allow for saving and transferring of images.

Optional Equipment

UVP offers a variety of optional equipment to support the needs of varying laboratory environments. Refer to
Replacement Parts and Accessories at the end of this manual for optional equipment part numbers.

Thermal Printer

The thermal printer provides archive quality, 256 grayscale prints and five optional cost-effective print
sizes.

Converter Plates

The UV/White Converter Plate allows imaging of non­fluorescent stained media with an ultraviolet transilluminator.
The converter plate is specially coated to convert 302nm UV
to white light.

The Visi-Blue™ Converter Plate (not shown) converts UV
to a safe 460-470nm wavelength designed for use with blue
excitation samples and SYBR Green, SYPRO Orange and
GFP stains.

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Connection for the
main darkroom
power

Connection for
jumper cord to the
transilluminator

Step-up ring

Diopter

Camera assembly bracket

Setup Instructions

Components

When unpacking the BioDoc-It2 Imaging System, the following items will be included:

1. BioDoc-It2 system with integrated transilluminator

2. Camera and zoom lens

3. Power, jumper and USB cables

4. Ethidium bromide (EtBr) emission filter

5. USB flash drive (8GB minimum)

6. Supporting documentation

7. USB flash drive containing VisionWorks touch Software and

8. USB flash drive containing VisionWorks Acquisition and Analysis Software (only for use on an external
computer)

Place the darkroom on a flat surface which can provide adequate support for up to 50 pounds.
WARNING: Do not attempt to perform any setup procedures while the system is plugged in or powered on

unless otherwise instructed.
CAUTION: Do not install the system in areas with high moisture, dust or high temperatures. Keep the

equipment away from motors or any other large magnetic equipment. This system is designed for indoor use
only.

Connecting the Power Cables

1. Plug the main power cable into the back of the
darkroom and the other end into a surge-protected
power outlet.

2. Connect the jumper cable from the darkroom to the rear
of the transilluminator.

Installing Emission Filters

To install the 50mm2 ethidium bromide (EtBr) filter and any other emission filters:

1. Carefully remove the filter from the protective plastic case, holding the filter at the edges to prevent
placing fingerprints on the glass surface.

2. The 2-position filter tray is located at the top of the system, below the camera lens hole. Place the
emission filter in the desired position by inserting the filter through the camera lens hole and into the
desired filter tray position. Note: The camera and lens must be removed to access the filter tray.

Additional and replacement emission filters are available through UVP. Refer to the Replacement

Parts and Accessories section of this manual for ordering information.

Camera Setup and Installation

The camera and zoom lens are assembled at the UVP factory.
Note: The zoom lens shipped may appear different than pictured.

1. Remove the cap from the lens.

2. If not already assembled, attach the step­up ring and diopter to the lens. The step­up ring and diopter will only fit one way.

3. Using the two brass thumb nuts provided,
secure the camera assembly bracket to
the darkroom bracket located on the top
of the BioDoc-It2. Ensure that a light-tight

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