UVP Benchtop UV Transilluminators User Manual

Benchtop UV Transilluminators

Instruction Manual

UVP, LLC Ultra-Violet Products Ltd.
2066 W 11th Street, Upland, CA 91786 Unit 1,Trinity Hall Farm Estate,
Tel: [phone] Nuffield Road Cambridge CB4 1TG UK
Fax: [phone] Tel: [phone] / Fax: [phone]

Web site: www.uvp.com

81-0249-01 Rev F

Benchtop UV Transilluminators Page 2

UV Blocking Cover may be
Select models include a

Power switch

UVP’s long life filters are

Introduction

The UVP Transilluminator offers the researcher uniform and intense sources of ultraviolet light
(radiation) in a compact package. The special design emits high intensity excitation UV wavelength
for back-illumination of transparent fluorescent materials. The 302nm UV back-illumination provides
a highly sensitive method to detect double-stranded nucleic acids that have been labeled with
fluorescent dyes such as ethidium bromide or acridine orange. Single stranded nucleic acids may
be detected, but with a lower excitation wavelength more sensitive for nucleic acid visualization
than the 365nm model.

The transilluminator is uniquely designed with increased UV intensity and uniformity, instant on
capabilities, no lamp flicker and reduced electrical consumption.

NOTE: Though the manual refers to the midrange UV waveband as 302nm, others refer to this
region as 300nm or 312nm. The spectral output of all these regions is the same.

A Word of Caution: UV Transilluminators are powerful sources of UV radiation that will cause
damage to unprotected eyes and skin. Before operating any unit, be sure all personnel in the area
are properly protected. If not using the transilluminator with an imaging system darkroom, a UV
Blocking Cover should be attached to the transilluminator. Even though this cover blocks the
ultraviolet radiation emitted by the unit, UV Blocking Eyewear should be worn as well.

UVP UV Transilluminator features:

included with the
transilluminator. The cover
is hinged and adjustable to
varying heights

control knob for selection of
wavelengths or intensity

available in a variety of
filter sizes from 15 x
15cm to 25 x 26cm

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Specifications

Single UV Benchtop Transilluminators

Single UV Benchtop Transilluminator models are designed with either single intensity or variable
intensity. Units are equipped with an electronic ballast .
Dimension: Width: 14” x Depth: 11” x Height: 4.8” (356 x 279 x 122mm)

Model Part Number Volt/Hz Wavelength Filter Size No. of Intensity Tubes Style

M-15 95-0455-01 100-115/60 302nm 15 x 15cm 4 x 8W Single
95-0455-02 230/50

M-15V 95-0456-01 100-115/60 302nm 15 x 15cm 4 x 8W Variable
95-0456-02 230/50

M-20 95-0447-01 100-115/60 302nm 20 x 20cm 4 x 8W Single
95-0447-02 230/50

M-20V 95-0452-01 100-115/60 302nm 20 x 20cm 4 x 8W Variable
95-0452-02 230/50

M-26 95-0457-01 100-115/60 302nm 21 x 26cm 4 x 8W Single
95-0457-02 230/50

M-26V 95-0458-01 100-115/60 302nm 21 x 26cm 4 x 8W Variable
95-0458-02 230/50

M-26XV 95-0413-01 100-115/60 302nm 25 x 26cm 4 x 8W Variable
95-0413-02 230/50

The mini single UV Benchtop Transilluminator us es a magnetic cor e/ co il design ballast. The
transilluminator dimensions are: 7.25”D x 10.25”W x 4.5”H. This model has a single intensity.

Model Part Number Volt/Hz/Amp Wavelength Filter Size No. of Tubes

M-10E 95-0180-01 115/60/0.7 302nm 10 x 10cm 5 x 6W
95-0180-02 230/50/0.6

95-0180-03 100/50-60/0.8

2UV Benchtop Transilluminators

Units are equipped with an electronic ballast. This model has a single intensity. The physical
dimensions of all models are:

Width: 14” x Depth: 11” x Height: 4.8” (356 x 279 x 122mm)

Model Part Number Volt/Hz Wavelength Filter Size #of Tubes/UV

LM-20 95-0449-01 100-115/60 302/365nm 20 x 20cm 4 x 8W/302nm
95-0449-02 230/50 4 x 8W/365nm

LM-26 95-0459-01 100-115/60 302/365nm 21 x 26cm 4 x 8W/302nm
95-0459-02 230/50 4 x 8W/365nm

Benchtop UV Transilluminators Page 4

When the UV Blocking Cover is not being
used, UV light may escape through the
holes dedicated to accepting the bracket

Safety Plug

4 x 8W/302nm

4 x 8W/302nm

3UV Benchtop Transilluminators

Units are equipped with an electronic ballast. The physical dimensions of all models are:
Width: 14” x Depth: 11” x Height: 5.4” (356 x 279 x 137mm)

Model Part Number Volt/Hz Wavelength Filter Size No. of Tubes/UV

LMS-20 95-0417-01 115/60 302/365/254nm 20 x 20cm
LMS-20 95-0417-02 230/50

LMS-26 95-0414-01 115/60 302/365/254nm 21 x 26cm
LMS-26 95-0414-02 230/50

4 x 8W/365nm
4 x 8W/254nm

4 x 8W/365nm
4 x 8W/254nm

Transilluminator Operation

Safety Precautions

pins of the UV Blocking Cover.

 Remove the black safety plugs from

their package

 Insert the safety plugs through the

holes as shown.

Set-Up

 Place the transilluminator on a level work surface. Be sure that an air space exists around

the bottom of the work surface. This space allows for the proper air circulation through the
unit.

 Plug the female end of the power cord into the transilluminator. For 230 volt models, or

those requiring special power cord connectors, ensure that the proper configuration of m ale
connector or plug has been properly connected to the power cord.

 Plug the male end of the power cord into a properly grounded electrical outlet. The proper

voltage of the transilluminator is found on the product information label. If using the
transilluminator with an imaging system, a jumper cable is required for connecting to the
darkroom. Refer to the imaging system documentation for additional instructions.

 The transilluminator may be equipped with a UV Blocking Cover. This cover allows the user

to safely view and work with the gel/sample on the glass surface while the transilluminator is
on (be sure to wear protective gloves when working with the gel/sample during this process).
To install, remove the brown protective paper from the cover. Insert the bracket pins on the
cover into the holes on the front of the transilluminator.

If you are not using the transilluminator with an imaging system darkroom, do not operate the unit

Benchtop UV Transilluminators Page 5

without securing the cover. If the cover is missing, a UV Blocking Faceshield must be worn to
avoid UV exposure to the skin. UV Blocking Eyewear should be worn even with the cover in
place to avoid accidental UV exposure.

Using the Transilluminator

Place gel/sample on the filter area. It is recommended to place the gels on a Gel-Tray to protect the
filter surface from cuts and scratches. It is recommended that gloves be worn to avoid contact with
gel and staining agents.

Press the ON/OFF switch to ON. The UV tubes within the unit should be glowing beneath the filter.
If using the transilluminator with an imaging system, the system’s main power is required to be in
the ON position.

When using a transilluminator with multiple UV wavelengths, dial the knob to the appropriate
wavelength setting.

When using the Variable Intensity models, use the variable intensity settings as follows:

 High: allows for UV excitation of fluorophores on gels for routine photography and for

excitation of gels with low sample concentratio n

 Medium: Excellent for viewing and quick single-band excision
 Low: Allows for positioning and preparation of gels, excising multiple bands and focusing

for photography

The UV Blocking Cover, optionally installed during setup, is adjustable to varying angles to provide
easier access to the filter surface. To adjust the angle, rotate the Phillips head screws (located on
the left sides of the cover’s hinges) clockwise until enough tension has been placed on the hinge to
hold the cover at the desired angle.

After viewing/photographing the sample, turn the transilluminator off.

Cleaning and Care of the Transilluminator

Clean unit surface with a damp soft cloth or sponge. Never use abrasive cleaners (can damage
the UV filter surface).

To protect the filter glass and minimize moisture and liquids on the glass, it is recommended that
you use a UV transmitting Gel-Tray. Refer to the Replacement Parts for ordering information.

Replacing Tubes in the Transilluminator

Disconnect the transilluminator for the electrical supply.
Remove the filter cover: Use a Phillips head screwdriver to remove the four screws on the sides of

the unit. Lift the filter cover off the unit.
Remove the reflectors on the left and right side of the unit: Slide the reflectors up out of the unit.
Remove the tube: Carefully rotate the tube and slide out of the socket. Replace with a new tube by

sliding the tube into the socket and rotating into place.
Insert the reflectors back into place and reattach the filter cover.

Benchtop UV Transilluminators Page 6

Maintenance/Repair/Technical Assistance

Replacement Parts/Accessories

For replacement parts or components not shown here, contach UVP Customer Service or place of
purchase. Please have the transilluminator model number available when you call.

Replacement Part Description Part Number Qty. Required

Filter Assembly (M-10) 38-0150-01 1
Filter Assembly (M-15) 38-0313-03 1
Filter Assembly (M-15V) 38-0313-09 1
Filter Assembly (M-20) 38-0313-01 1
Filter Assembly (M-26) 38-0313-02 1
Filter Assembly (M-20V, LM-20, LMS-20) 38-0313-07 1
Filter Assembly (M-26V, LM-26, LMS-26) 38-0313-08 1
Filter Assembly (M-26XV) 38-0163-04 1
Cover, UV blocking (M-10E) 19-0114-01 1
Cover, UV blocking (all other models) 19-0121-01 1
UV Tube, 6 Watt, 302nm midrange (FL6E) – TM-10E 34-0044-01 5
UV Tube, 8 Watt, 302nm midrange (FL8E) 34-0042-01 4
Tube, 8 Watt, 365nm longwave UV (F8T5/BL) 34-0006-01 4
Tube, 8 Watt, 254nm shortwave UV 34-0007-01 4
Ballast, electronic (M-15, M-20, M-26, LM-20, LM-26) 42-0068-04 (115V) 1
42-0068-06 (230V) 1
Ballast, electronic, variable (M-15V, M-20V, M-26V, M-26XV) 42-0067-04 (115V) 1
42-0067-06 (230V) 1
Ballast, (M-10E) 42-0005-01 5
Fuse, 2 amp, 250V, Slo-Blo (all voltages) 56-0002-01 2

Accessories Description Part Number

Gel-Cutter 85-0002-01
Gel-Scooper 85-0006-01
Gel-Tray, UV Transmitting, Sm. 11.5”W x 9”D (29 x 23cm) 85-0007-01
Gel-Tray, UV Transmitting, Lg. 16.5”W x 10.5”D (42 x 27cm) 85-0005-01
Gel-Tray, High Performance UV Transmitting (25 x 26cm) 38-0296-03
Gel-Ruler, UV Fluorescing 85-0003-01
Spectacles, UV Blocking 98-0002-01
Goggles, UV Blocking 98-0002-02
Faceshield, UV Blocking 98-0002-04

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Technical Support

UVP offers technical support for all of its products. If you have any questions about the product’s
use or, operation, please contact UVP’s offices at the following locations.

If you are in North America, South America, If you are in Europe, Africa, the Middle East,
East Asia or Australia: Western Asia:

 Call [phone] or [phone]  Call [phone] Customer
Customer Service regular business regular business days, between 9 am
days, between 7 am and 5 pm PST and 5:30 pm

 E-Mail: [e-mail]  E-Mail: [e-mail]

 Fax Customer Service: [phone]  Fax Customer Service: [phone]

 Write to : UVP, L LC  Write to : Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Est, Nuffield Rd

Upland, CA 91786 USA Cambridge CB4 1TG UK

Note: A Returned Goods Authorization (RGA) number must be obtained from UVP Customer

Service before returning any product.

Warranty

UVP, LLC warrants its Ultraviolet Transilluminators to be free of detects in materials and workmanship
for a period of two (2) years from date of purchase. The foregoing warranty of UVP shall be of no force
and effect if buyer has modified or damaged the product. Tubes and filters are warranted for 90 days.

All warranties or merchantability and fitness for any purpose and all other warranties, expressed or
implied, except those expressly set forth herein, are deemed waived and excluded.

UVP’s duty under the warranty is limited to replacement and/or repair of the defective part at the option
of UVP, LLC. UVP shall not be liable for any expenses or damages incurred by the purchaser except
as expressly set forth herein, and in no event shall UVP be liable for any special, incidental or
consequential damages of any kind. This warranty does not supersede any statutory rights that may be
available in certain countries.

UVP … Providing Quality Products for the Researcher Since 1932