UVP Benchtop UV Transilluminator User Manual

Benchtop UV Transilluminator

Operating Instructions

IMPORTANT: READ THESE INSTRUCTIONS BEFORE OPERATING YOUR UVP

TRANSILLUMINATOR TO FAMILIARIZE YOURSELF WITH ITS OPERATION.

UVP, LLC 2066 W 11th Street, Upland, CA Ultra-Violet Products Ltd., Trinity Hall Farm Estate,
Tel: [phone] Unit 1, Nuffield Road, Cambridge CB4 1TG UK
Fax: [phone] Tel: [phone] / Fax: [phone]

Web Site: www.uvp.com

81-0118-01 Rev D

CONTENTS

Introduction.......................................................................................................................... 1

Important Safety Information ................................................................................................. 1

Description/Specifications ..................................................................................................... 1

Single UV Benchtop Transilluminator
2UV Benchtop Transilluminators
3UV Benchtop Transilluminators

Ultraviolet Spectral Output Charts ......................................................................................... 3

Transilluminator Operation ..................................................................................................... 3

Single UV Benchtop Transilluminators
2UV Benchtop Transilluminators
3UV Benchtop Transilluminators

Maintenance/Repair/Technical Assistance ............................................................................ 4

Single UV Transilluminator Schematic
2UV Transilluminator Schematic
3UV Transilluminator Schematic

Replacing the UV Tubes........................................................................................................ 7

In the Single UV Benchtop Transilluminator
In the 2UV Benchtop Transilluminators
In the 3UV Benchtop Transilluminators

Cleaning the Transilluminator................................................................................................. 7

Replacement Parts and Accessories ..................................................................................... 8

Warranty .............................................................................................................................. 9

UVP ... Providing quality products for the researcher since 1932 ......................................... 9

Benchtop UV Transilluminators Page 1

Introduction

The UVP Benchtop Transilluminator Series offers the researcher uniform and intense sources of ultraviolet
light (radiation) in a compact package. Their special design emits either one, two or three high intensity
excitation UV wavelengths for back-illumination of transparent fluorescent materials.

The 302nm UV back-illumination included in all Benchtop Transilluminators provides a highly sensitive
method to detect double-stranded nucleic acids that have been labeled with fluorescent dyes such as
ethidium bromide or acridine orange. Single stranded nucleic acids may be detected, but with a lower
excitation wavelength more sensitive for nucleic acid visualization than the 365nm model.

NOTE: Though UVP refers to the mid-range UV waveband as 302nm, others refer to this region as 300nm
or 312nm. The spectral output of all these regions is the same.

Important Safety Information

A Word of Caution: All UVP Benchtop Transilluminators are powerful sources of UV radiation that will cause

damage to unprotected eyes and skin. Before operating any unit, be sure all personnel in the area are properly
protected. It is preferable that the transilluminator be installed and operated in a darkroom where access and
exposure is limited while the unit is in operation. If a darkroom is unavailable, UVP offers a Darkroom which
provides protection from accidental exposure. For part numbers of the Darkroom, contact UVP. Each transillu­minator is shipped a UV Blocking Cover. Even though this cover blocks the ultraviolet radiation emitted by the
unit, UV Blocking Eyewear should be worn as well. UVP has a complete line of UV Blocking Eyewear:
Spectacles, Goggles, and Faceshield designed for this purpose. For part numbers, see Section 9.

Description/Specifications

Single UV Benchtop Transilluminator

The dimensions of the Single UV Benchtop Transilluminator models are:
Width: 13.25” (33.7cm) / Depth: 9.50” (24.1cm) / Height: 4.75” (12.1cm)

Model Part Number Volt/Hz/Amp Wavelength Filter Size # of Tubes Intensity Style

M-15E 95-0218-01 115/60/0.7 302nm 15 x 15cm 5 x 8W Single

95-0218-02 230/50/0.6
95-0218-03 100/50-60/0.8

M-15 95-0219-01 115/60/0.7 302nm 15 x 15cm 5 x 8W Hi/Lo

95-0219-02 230/50/0.6
95-0219-03 100/50-60/0.8

M-20E 95-0210-01 115/60/0.7 302nm 20 x 20cm 6 x 8W Single

95-0210-02 230/50/0.6
95-0210-03 100/50-60/0.8

M-20 95-0216-01 115/60/0.7 302nm 20 x 20cm 6 x 8W Hi/Lo

95-0216-02 230/50/0.6
95-0216-03 100/50-60/0.8

Nominal

M-26E 95-0221-01 115/60/0.7 302nm 21 x 26cm 6 x 8W Single

95-0221-02 230/50/0.6
95-0221-03 100/50-60/0.8

M-26 95-0222-01 115/60/0.7 302nm 21 x 26cm 6 x 8W Hi/Lo

95-0222-02
95-0222-03

Benchtop UV Transilluminators Page 2

2UV Benchtop Transilluminator

The physical dimensions of all models are:
Width: 13.25” (33.7cm)

Depth: 9.50” (24.1cm)
Height: 4.75” (12.1cm)

Nominal

Model Part Number Volt/Hz/Amp Wave-Length Filter Size #of Tubes/ Wavelength

LM-20E 95-0212-01 115/60/0.7 302nm/365nm 20 x 20cm 6 x 8W/302nm 5 x 8W/365nm

95-0212-02 230/50/0.6
95-0212-03 100/50-60/0.8

LM-26E 95-0223-01 115/60/0.7 302nm/365nm 21 x 26cm 6 x 8W/302nm 5 x 8W/365nm

95-0223-02 230/50/0.6
95-0223-03 100/50-60/0.8

3UV Benchtop Transilluminator

The physical dimensions of all models are:
Width: 14.0” (35.6cm)

Depth: 11.0” (27.9cm)
Height: 5.4” (13.7cm)

Nominal

Model Part Number Volt/Hz/Amp Wavelength Filter Size #of Tubes/ Wavelength

LMS-20E 95-0220-01 115/60/0.85 302/365/254nm 20 x 20cm 6 x 8W/302nm
LMS-20E 95-0220-02 230/50/0.75 5 x 8W/365nm
LMS-20E 95-0220-03 100/50-60/0.90 5 x 8W/254nm

LMS-26E 95-224-01 115/60/0.85 302/365/254nm 21 x 26cm 6 x 8W/302nm
LMS-26E 95-224-02 230/50/0.75 5 x 8W/365nm
LMS-26E 95-224-03 100/50-60/0.90 5 x 8W/254nm