UVP Automated BioLite User Manual

Automated BioLite MultiSpectral Light Source

Installation and User Instructions

™

UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK Phone: (800) 452-6788 Phone: +44(0)1223-420022 Fax: (909) 946-3597 Fax: +44(0)1223-420561

Web Site: www.uvp.com

81-0276-01 Rev F

Automated BioLite MultiSpectral Light Source 2

Introduction .................................................................................................................................................................. 3

System Components ................................................................................................................................................... 4

Specifications ............................................................................................................................................................ 4

Output Spectrum ....................................................................................................................................................... 5

Wavelength (nm) ....................................................................................................................................................... 5

Setup Instructions ........................................................................................................................................................ 6

Using the Light Source .............................................................................................................................................. 10

Service Procedures .................................................................................................................................................... 12

Bulb Replacement ................................................................................................................................................... 12

Return Procedure .................................................................................................................................................... 13

Replacement Parts and Accessories ....................................................................................................................... 13

Troubleshooting ....................................................................................................................................................... 13

Care and Cleaning .................................................................................................................................................. 14

Technical Support ................................................................................................................................................... 14

Automated BioLite MultiSpectral Light Source 3

Introduction

The Automated BioLite MultiSpectral Light Source is a 150 watt quartz halogen visible light source designed for use with select UVP Advanced Imaging Systems. The unit features a closed optical path to tightly control the output spectrum, allowing consistent and repeatable measurements with superior signal to noise. The Automated BioLite features a six position dimmer permitting variable intensity, and the integrated filter wheel accommodates up to eight excitation filters. System control is via either the Visi onWorks software interface or switches located on the front of the unit.

The unit’s fiber optic bundle, designed uniquely for the BioLite, direc ts lighting to the darkroom. Refer to the components list included with the BioLite and imaging system to determine the appropriate assembly instructions.

Automated BioLite MultiSpectral Light Source 4

Fiber Optic Cable

Filter Access Door

(Side)

Press Button to

Transillumination

Illumination Ports: Left for

Master Power

Switch

Halogen Bulb Power

Light Intensity Selector

Filter Selector Switch and

Position Indicator

System Components

Refer to the packing slip and schematic shown below for specific parts and components included with the BioLite MultiSpectral Light Source.

Button: Press to turn bulb

on (red light) or

off/standby (green)

Switch Between Epi

Illumination and

Switch and Indicator

epi illumination and right

for transillumination

(cannot be used

simultaneously)

Specifications

Color Temperature: 3250°K Bulb Type: 150 Watt Halogen Type EKE Ferrule Dimension: 0.718 inch (18.2 mm) Physical Dimensions:

Height 9.6 Inches (22 cm) Width 9.5 Inches (18 cm) Depth 10 Inches (25 cm)

Power Requirements:

115V 1.8 Amps max, 50/60 Hz 230V 0.9 Amps max. 50/60 Hz 100V 2.1 Amps max, 50/60 Hz

Weight 13.75 pounds (4 kg)

Automated BioLite MultiSpectral Light Source 5

Intensity

Output Spectrum

The graph below displays the spectral emission curve for the halogen light source within the BioLite (shown without filtering):

Safety Information

WARNING: The device will overheat if the fan outlet on the back of the BioLite is blocked. WARNING: The quartz halogen bulb becomes very hot with use. Do not open the bottom access panel

while the BioLite is running or without allowing it to fully cool. CAUTION: Never touch the inner portion of the quartz halogen bulb. If it accidentally comes in contact with

skin, carefully clean the glass with rubbing alcohol and a clean, lint fr ee clot h. CAUTION: Never look into the output port or fiber optic bundle of the BioLite when it is on, as the intense

light may hurt your eyes.

Wavelength (nm)

Automated BioLite MultiSpectral Light Source 6

USB Cable

Power Cord

Ridge

Side of

Setup Instructions

BioLite MultiSpectral Light Sourc e Setup

1. Plug the power cord into the receptacle on the back of the unit and t he other end to a power outlet. Connect the USB cable to the BioLite and the other end to an available port on the computer.

2. Position the BioLite to the left of the darkroom.

Excitation Filter Installation

Filters are installed in a metal black casing from the UVP fac tory . To ins tall the filters into the BioLite filter wheel:

1. Hold the filter assembly so that it is positioned vertically with the filter hol der’s ridge facing toward the front of the BioLite as shown below.

BioLite

2. Slide the filter into the filter wheel as shown below. The small metal tab on the filter holder will magnetically hold the filter in place within the BioLite. Note: Do not force the filter into the BioLite wheel slot. If the filter is not fitting smoothly, ensure that the orientation of the fi lter assembly is correct.

3. To access the other filter positions, manually rotate the filter wheel with the BioLite powered off.

Automated BioLite MultiSpectral Light Source 7

Epi Light Guides

Stopper

Fiber Optic Cable

Plug the Fiber

Attach light guide s to these

Epi Light Guide Installation (BioSpectrum®)

1. Remove the epi light covers at the top of the darkroom.

2. Remove one stopper at the left side of the darkroom.

3. Thread the knurled (non-smooth side) of the fiber optic cable from the inside of the darkroom through the hole created by removing the stopper until the large bulb separating the smooth and rough side of the fiber optic cable can no longer be pushed through the opening.

4. The light guides should be on the inside of the darkroom. Make sure the fiber optic cables extend from the light guide toward the front of the darkroom (toward you). Match the drilled holes in the light guide holders to the protruding screws on the epi light structure. Use the brass thumb nuts to secure the light guides to the darkroom.

screws at top of darkroom using the brass thumb nuts

5. Remove the rubber cap from the input side of the fiber optic trunk and plug the cable into the left port on the BioLite unit.

Optic Cable into

the left port

Automated BioLite MultiSpectral Light Source 8

Light emitting

platform tray

Cable faces

the darkroom

Light guide fits

bracket

Epi Light Guide Installation (GelDoc-It

1. Remove one stopper at the left side of the darkroom.

2. Remove any existing plates or screws from the m ounting bracket so that the epi light guide bracket appears as follows:

4. Position the light guide in the mounting bracket as shown below. Make sure the cable protruding from the light guide faces toward the front of the darkroom (toward the darkroom door) and that the light emitting portion of the light guide is facing toward the platform tray.

®2/TS2

and ChemiDoc-It

®2/TS2

)

the front of

5. Attach the mounting bracket plate to the mounting bracket using two of the screws provided, as shown below. Make sure the tab on the plate faces up.

6. Repeat the process for the other light guide within the darkroom.

snugly in mounting

portion of the light

guide is facing

Automated BioLite MultiSpectral Light Source 9

Plug the Fiber

7. Remove the rubber cap from the input side of the fiber optic trunk and plug the cable into the left port on the BioLite unit.

Optic Cable into

the left port

Fiber Optic Light Table (Transilluminator) Installation

1. Remove one stopper at the left side of the darkroom.

2. If the imaging system already contains a transilluminator, unplug and remove the transilluminator.

3. Insert the fiber optic light table transillumi nator in the imaging system.

4. Thread the knurled (non-smooth side) of the fiber optic cable from the inside of the darkroom through the hole created by removing the stopper until the large bulb separating the smooth and rough side of the fiber optic cable can no longer be pushed through the opening.

5. Remove the rubber cap from the input side of the fiber optic trunk and plug the cable into the left port on the BioLite unit.

Automated BioLite MultiSpectral Light Source 10

Using the Light Source

1. Set the power switch on the front of the system to the ON (I) position.

2. Use the VisionWorksLS “BioLite” module (shown below) to adjust the various BioLite settings:

a. Filter: Select the desired excitation filter position from the dropdown menu. b. Light Engine: Turn the BioLite bulb on or off using the radio buttons (note that the

BioLite’s green power switch must be in the ON position to use the lighting controls). When the bulb is on, the l ower indicator light to the left of the switch on the front of the BioLite will glow red.

c. Intensity: This setting regulates the amount of illumination i n six increments. The relative

output levels are specified in the chart below:

Intensity Setting Relative Intensity

6 100% 5 50% 4 40% 3 25% 2 12% 1 0%

Light Table: Select between either Epi (overhead) or Trans (base lighting) depending on the application. Note that both the epi and transillumination cannot be used simultaneously.

NOTE: If using the darkroom to perform light-tight experiments such as capturing a chemiluminescence sample for more than two minutes, use the black rubber cap to cover the end of the fiber optic cable. Exposing an image for less than two minutes with the rubber cap removed will not impact the resultant image, so long as the fiber optic cable is installed in the BioLite. Without capping the fiber optic cable, light may enter the darkroom through the end of the fiber optic cable, thus affecting the results of an extended resolution image.

3. It is possible to adjust the BioLite’s settings using the switches located on the front of the BioLite. Note, however, that any settings modified using the switches will NOT be reflected in VisionWorksLS’ BioLite software interface.

Automated BioLite MultiSpectral Light Source 11

Fiber Optic Lighting Light Source Switch

Light Intensity Switch

Filter Position Switch

Selector

a. Fiber Optic Lighting: Press this button to select either Epi or Transillumination lighting.

Note that both epi and transillumination cannot be used simultaneously.

b. Light Source: Press this button to turn the halogen light bulb on or off. When the bulb is

on, the lower indicator light to the left of the switch will glow red.

c. Light Intensity: Press the rocker switch toward Max to increase the relative intensity of

the lighting. Inversely, press the rocker switch toward Min to decre ase the relativ e intensity of the lighting. Lighting is provided in six increments, indicated on the LCD display to the left of the rocker switch .

d. Filter Position: Press the rocker switch toward + to move to the next exc i tation filter

position. Inversely, press the rocker switch toward – to move to the previous excitation filter position. There are a total of eight filter positions; the current position will be indicated on the LCD display to the left of the rocker switch.

Automated BioLite MultiSpectral Light Source 12

Brass Thumb Nut

Bulb Access Door

Service Procedures

Bulb Replacement

The BioLite’s Type EKE 150 watt halogen bulb has a standard life of 200 hours. It must be replaced with the same bulb type only. The UVP part number is 34-0088-01.

The BioLite bulb replace ment pr oce dure is as foll ow s:

1. After allowing the unit sufficient time to cool after last use (at least 1/2 hour), unplug the BioLite

from its power source.

2. From the bottom of the unit, remove the brass thumb nut and remove the bulb access door.

3. Remove the bulb using the mechanism built into the bulb holder. Grasp the chrome wire to the

left of the bulb and push it in the direction of the base of the bulb. The bulb will pop out of the connector.

4. Return the bulb removal mechanism to its original, downward position.

Automated BioLite MultiSpectral Light Source 13

5. Insert the replacement EKE halogen 150 watt bulb, ensuring that it is fully seated.

6. Replace the cover and secure with the brass nut before plugging unit back in or turning it on.

Return Procedure

A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service before returning any product. See “Technical Support” below for contact information.

Replacement Parts and Accessories

To order accessories or replacement parts for the BioLite MultiSpectral Light Source, contact UVP’s offices.

Part Description Part Number

Type EKE 150 Watt Halogen Bulb 34-0088-01 370 – 430 nm Filter (for BFP, CFP, Etc.) 38-0319-01

422 – 478 nm Filter (for GFP, Cy2, Etc.) 38-0319-02 500 - 680 nm Filter (for Cy 5.5) 38-0319-07

Blue Filter (to enhance red and yellow objects) 38-0319-03 Red Filter (darkens green and blue objects) 38-0319-04 Green Filter (increases for red or blue objects) 38-0319-05 Yellow Filter (safelight for photo resist materials) 38-0319-06

Other standard and custom filters are available for a variety of imaging applications. Contact UVP

or visit www.uvp.com for further information.

Troubleshooting

Bulb Does Not Illuminate

1. Recheck the main power cord connection to the BioLite unit.

2. Check the fuses located at the back of the unit next to the power port. A small flat-head screwdriver or similar tool will be required. Turn the fuseholder cap counterclockwise and the fuse holder will pop out. Inspect the thin wire within the glass fuse to see if there is a break in the wire. If so, replace the fuse(s). If fuses are blowing repeatedly, contact UVP Technical Support for additional troubleshooting.

3. The bulb may be out of position. Reseat the bulb to reestablish the electric al conn ect ion by following the “Bulb Replacement” instruction earlier in this manual.

4. The bulb may be burned out. Follow the “Bulb Replacement” instruction earlier in this manual.

Automated BioLite MultiSpectral Light Source 14

Care and Cleaning

Use only mild soap or detergent solution for cleaning. Do NOT use oil- or petroleum-based cleaners for the cabinet. Ensure that the system is turned OFF and unp lugg e d during cle anin g.

Technical Support

UVP offers free lifetime technical support on all of its products and software. Should you have any questions regarding the product’s use, operation or repair, contact UVP’s offices at the locations below, or visit www.uvp.com.

If you are in North America, South

America, East Asia or Australia:

Call (800) 452-6788 or (909) 946-

3197, and ask for Technical Support

during regular business days, between

7:00 am and 5:00 pm, PST.

E-mail your message to:

info@uvp.com

Fax Customer Service, and send it to

(909) 946-3597

Write to: UVP, LLC 2066 W. 11th

Street, Upland, CA 91786 USA

If you are in Europe, Africa, the

Middle East of Western Asia:

Call +44(0) 1223-420022, and ask for

Customer Service during regular

business days between 9:00 am and

5:30 pm.

E-mail your message to: uvp@uvp.co.uk

Fax Customer Service, and send it to:

+44(0) 1223-420561

Write to: Ultra-Violet Products Ltd

Unit 1, Trinity Hall Farm Estate, Nuffield

Road, Cambridge CB4 1TG UK

VisionWorks, BioSpectrum, GelDoc-It and ChemiDoc-It are registered trademarks of UVP, LLC. BioLite is a trademark of UVP, LLC.