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81-0357-01
User Manual
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UVP 81-0357-01 User Manual

PCR Workstations and Cabinets
Installation and User Instructions
Web Site: www.uvp.com

81-0357-01 Rev STD

PCR Workstations and Cabinets Page 2
UV1 PCR Cabinet
UV2 PCR Cabinet
UV1 PCR Workstation
UV2 PCR Workstation
95-0437-01 115V
95-0437-03 100V
95-0436-01 115V
95-0436-03 100V
95-0367-01 115V
95-0367-03 100V
95-0439-01 115V
95-0439-03 100V
Light Sources:
Light Sources:
Light Sources:
Light Sources:
Chamber
Features include:
Features include:
UV/Air Circulator
Features include:
Optional: UV/Air Circulator
Features include:
UV/Air Circulator
Dimensions:
in. (500 x 544 mm)
Dimensions:
in. (500 x 544 mm)
Dimensions:
in. (706 x 544 mm)
Dimensions:
in. (706 x 544 mm)

Introduction

The UV1 and UV2 PCR Hoods create an ideal environment for preparing PCR and other samples by reducing the chance of contamination. The built-in high intensity shortwave (254nm) UV provides a source for inactivation of DNA between experiments. Additional contamination control is provided by the specially-coated stainless steel and aluminum design that maintains antimicrobial efficacy. The surface resists growth of destructive bacteria, molds and fungi. The Makrolon assembly blocks UV below 400nm.
In addition to the UV1/UV2 PCR Hood features, HEPA/UV3 PCR Hoods provide additional protection by blowing filtered air, treated by the HEPA/UV system, into the PCR chamber.
The UV/Air Circulator, included as standard on UV2 and HEPA/UV3 PCR Hoods and optional on the UV1 PCR W orkstation (95-0367-xx only), eliminates amplicons of DNA or genomic DNA from a previously-dispensed experiment. For ordering information, refer to the “Maintenance and Replacement Parts/Accessories” section.
®
panel

Safety Information

The PCR Hoods are designed with functionality, reliability and safety in mind. The Hoods provide shortwave (254nm) UV which is a powerful source of UV radiation that will cause damage to unprotected eyes and skin. Before operating, ensure that all personnel are properly protected and that the instructi ons for the use of this equipment are followed. A safety shut-off switch automatically turns off the UV light when the door is open, protecting users from UV exposure. In addition, the Makrolon panels are specially formulated to block UV wavelengths below 400nm.
Disconnect the power supply before assembling or servicing the PCR Hoods.
®
95-0437-02 230V UK 95-0437-04 230V Euro
254nm 8-watt UV:
Chamber
White 8-watt
Chamber
Two power outlets Two small shelves UV Timer Antimicrobial coated
stainless steel (work
surface) & aluminum Makrolon block below 400nm
Exterior: 28.7H x 21.4W x 24D in. (729 x 544 x 610 mm) Interior: 19.7W x 21.4D

Specifications

STANDARD UV (UV1 and UV2) PCR MODELS
95-0436-02 230V UK 95-0436-04 230V Euro
254nm 8-watt UV:
White 8-watt:
Two power outlets Two small shelves UV Timer Antimicrobial coated
®
panels
Makrolon
Exterior: 28.7H x 21.4W x 24D in. (729 x 544 x 610 mm) Interior: 19.7W x 21.4D
Chamber UV/air circulator
Chamber
stainless steel (work surface) & aluminum
®
panels block
below 400nm
95-0367-02 230V UK 95-0367-04 230V Euro
254nm 25-watt UV:
Chamber
White 15-watt:
Chamber
Four power outlets Two shelves UV Timer Antimicrobial coated
stainless steel (work surface) & aluminum
®
Makrolon
below 400nm
Exterior: 28.7H x 29W x 24D in. (729 x 737 x 610 mm) Interior: 27.8W x 21.4D
panels block
95-0439-02 230V UK 95-0439-04 230V Euro
254nm 25-watt UV:
Chamber
254nm 8-watt UV:
UV/air circulator
White 15-watt:
Four power outlets Two shelves UV Timer Antimicrobial coated
stainless steel (work surface) & aluminum
®
Makrolon
below 400nm
Exterior: 28.7H x 29W x 24D in. (729 x 737 x 610 mm) Interior: 27.8W x 21.4D
panels block
PCR Workstations and Cabinets Page 3
Light Sources:
Chamber
Light Sources:
Chamber
Features include:
UV/Air Circulator
Features include:
UV/Air Circulator
Antimicrobial coated stainless steel (horizontal work surface) and aluminum
Exterior
Aluminum powder coated
HEPA/UV3 PCR MODELS
HEPA UV3 PCR Cabinet HEPA UV3 PCR Workstation
95-0434-01 115V 95-0434-02 230V UK 95-0434-04 230V Euro 95-0434-03 100V
254nm 8-watt UV:
Filter area Chamber UV/air circulator
White – 8-watt:
Three stage filters:
Pre-filter Carbon filter HEPA filter
Two power outlets Two small shelves UV Timer Antimicrobial coated stainless steel (work
surface) and aluminum
®
Makrolon below 400nm
Dimensions:
Exterior:
32.5H x 21.4W x 24D in. (826 x 544 x 610 mm) Interior: 19.7W x 21.4D in. (500 x 544 mm)
panels block
95-0438-01 115V 95-0438-02 230V UK 95-0438-04 230V Euro 95-0438-03 100V
254nm UV:
Filter area 8-watt Chamber 25-watt UV/air circulator 8-watt
White – 8-watt:
Three stage filters:
Pre-filter Carbon filter HEPA filter
Four power outlets Two shelves UV Timer Antimicrobial coated stainless steel (work
surface) and aluminum
®
Makrolon below 400nm
Dimensions:
Exterior:
32.5H x 29W x 24D in. (826 x 737 x 610 mm) Interior: 27.8W x 21.4D in. (706 x 544 mm)
panels block
Shelves
Construction Dimensions (Workstation) Dimensions (Cabinet)
Construction
Interior
Door and Side Panels
Electrical
115V - 60 Hz, 230V - 50 Hz, 100V - 50/60Hz
Two - Formed antimicrobial-coated aluminum 13”W x 4.2”D (330 x 107mm)
8.75” x 4”D (222 x 102mm)
Makrolon® panel assembly Blocks UV under 400nm
PCR Workstations and Cabinets Page 4
Max
95-0437-01
95-0438-05
115V
230V
9.1
8.7

Operational Ratings

• PCR Hoods are intended to be used indoors only
• Altitude must not exceed 2,000m
• Ambient temperature must not exceed 5°C to 40°C (41°F to 104°F)
• Relative humidity must not exceed 80% for temperatures up to 31°C (88°F) decreasing linearly to
50% relative humidity at 40°C (104°F)
• Mains supply voltage fluctuations must not ex ceed ±10% of the nominal voltage
• Pollution rating 2 or better for laboratory areas
Part Number Voltage
95-0437-02 95-0437-04 95-0437-03 95-0437-05 95-0436-01 95-0436-02 95-0436-04 95-0436-03 95-0436-05 95-0367-01 95-0367-02 95-0367-04 95-0367-03 95-0367-05 95-0439-01 95-0439-02 95-0439-04 95-0439-03 95-0439-05 95-0434-01 95-0434-02 95-0434-04 95-0434-03 95-0434-05 95-0438-01 95-0438-02 95-0438-04 95-0438-03
230V 230V 100V 230V 115V 230V 230V 100V 230V 115V 230V 230V 100V 230V 115V 230V 230V 100V 230V 115V 230V 230V 100V 230V 115V 230V 230V 100V
Amps
8.8
8.8
9.1
8.8
9.1
8.7
8.7
9.1
8.7
8.8
8.6
8.6
8.8
8.6
9.1
8.7
8.7
9.1
8.7
9.1
8.7
8.7
9.1
8.7
9.1
8.7
8.7
9.1
PCR Workstations and Cabinets Page 5

Assembling the PCR System

Carefully remove the PCR Hood components from the packaging. CAUTION: Use care when unboxing the PCR Hood, as staples with sharp ends may have been used in packaging.
Follow the assembly instructions provided in this manual. NOTE: Place the PCR Hood Base on a level surface large enough to accommodate the entire Base. The surface must be level to ensure proper alignment of the Makrolon
Required Tools
• Philips head screwdriver (motorized, if available)
• Flathead screwdriver (motorized, if available)
• Gloves, to reduce transfer of fingerprints to PCR Hood components
• Two people for assembly and lifting of the equipment
NOTE: Assembly drawings show the 95-0436-01 UV2 PCR Cabinet. However, these instr uctions apply for all PCR models.
Outer Back Panel
1. Separate the Inner and Outer Back Panels by removing the 4 screws, as shown: NOTE: The Outer and Inner Back Panels and Shelf Bracket are assembled at the factory for
shipping purposes.
®
panels.
2. Loosen the two screws on the Base Assembly, as shown:
PCR Workstations and Cabinets Page 6
Screw
Screw
Base
Outer Back Panel. Note the notch in
the top of the panel allows room for
NOTE: It is advised to use the remaining bubble wrap to protect the antibacterial-coated surfaces during assembly.
3. Attach the Outer Back Panel to the Base Assembly with two screws, as shown below. Leave the screws loose (do not fully tighten at this time).
the wires.
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