UVP 81-0345-01 User Manual

TS2

GelDoc-It

®

& ChemiDoc-It

Imagers

Installation and User Instructions

TS2

®

UVP, LLC Ultra-Violet Products Ltd.

2066 W. 11th Street Unit 1, Trinity Hall Farm Estate
Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK
Phone: [phone] Phone: [phone]
Fax: [phone] Fax: [phone]

Web Site: www.uvp.com

81-0345-01 Rev F

GelDoc-It

TS2

and ChemiDoc-It

TS2

Imagers 2

Table of Contents

Introduction .................................................................................................................................................................. 3

System Components ................................................................................................................................................... 4

Specifications ............................................................................................................................................................ 5

Built-In Touch Screen Computer ............................................................................................................................... 5

Cameras and Lenses ................................................................................................................................................ 5

Ethidium Bromide (EtBr) Emission Filter ................................................................................................................... 5

Darkroom................................................................................................................................................................... 6

Transilluminator ......................................................................................................................................................... 6

LCD Touch Screen .................................................................................................................................................... 6

TS2 Software ............................................................................................................................................................. 6

Fluorescent Focus Target ......................................................................................................................................... 6

Optional Equipment ................................................................................................................................................... 7

Setup Instructions ........................................................................................................................................................ 9

Components .............................................................................................................................................................. 9

Connecting the Power Cables ................................................................................................................................... 9

Installing Emission Filters ........................................................................................................................................ 10

Using the System ....................................................................................................................................................... 11

Powering Up the System ......................................................................................................................................... 11

Preparing the Transilluminator for Use .................................................................................................................... 11

Operating the TS2 Software Interface ..................................................................................................................... 12

Using the UV Gel Viewer Window ........................................................................................................................... 12

Image Focusing ....................................................................................................................................................... 12

Image Zooming ....................................................................................................................................................... 13

Touch Screen Interface ............................................................................................................................................. 14

Setting User Preferences ........................................................................................................................................ 14

Changing Lighting and Filter Settings ...................................................................................................................... 16

Identifying the Touch Screen Buttons and Functions .............................................................................................. 17

Using Templates ..................................................................................................................................................... 25

Connecting to a Network ......................................................................................................................................... 26

Installing Drivers or Additional Software .................................................................................................................. 26

Service Procedures .................................................................................................................................................... 27

Return Procedure .................................................................................................................................................... 27

Replacement Parts and Accessories ....................................................................................................................... 27

Troubleshooting ....................................................................................................................................................... 27

Technical Support ................................................................................................................................................... 28

GelDoc-It

TS2

and ChemiDoc-It

Introduction

The GelDoc-It
fluorescent gel images.

In addition to gel documentation capabilities, the ChemiDoc-It
scientific-grade CCD camera allowing users to capture and document chemiluminesc ent bl ot image s,
including Western blots.

The TS2 Imagers are self-contained, light-tight imaging systems with a built-in 15.6” touch screen and
computer and TS2 Software for image acquisition.

The darkroom has a UV-blocking gel viewer window, built-in overhead epi white lighting, a UV
transilluminator and a five-position slide-out emission filter wheel with an ethidium bromide (EtBr) emission
filter included as standard. Save images to the internal hard drive, to an external USB storage device or to a
network drive for later quantitative analysis or enhancement for publication.

UVP’s VisionWorks
colony plates. VisionWorksLS requires an external computer and cannot be operated directly on the TS2’s
integrated computer.

*System/software configurations may vary by country. Contact UVP or authorized distributor for details.

TS2

Imagers 3

TS2

Imager is a high resolution imaging system capable of capturing and documenting

TS2

Imager is equipped with a cooled,

®

LS Analysis software is included* with all TS2 Imagers for analysis of gels, blots and

GelDoc-It

Camera and Lens

Hood

15.6” Touch Screen
Computer

Computer Power

Wide Access Door

USB Access Port

Built-In Overhead

White Lights

Five Position Slide-

Wheel

UV Gel Viewer

Window

Transilluminator on

Roll-Out Tray

UV Safety Interlock

Switch & Override

Black Chemi Tray

versions only)

BioLite™ Access Port

Power Indicator

Lights

Stylus with Adhesive

Holder (Not Shown)

TS2

and ChemiDoc-It

TS2

Imagers 4

System Components

Refer to the packing slip and pictured components for specific parts and components included with the
system.

Built Into Darkroom

Out Emission Filter

(ChemiDoc-It

TS2

GelDoc-It

TS2

and ChemiDoc-It

TS2

Imagers 5

Specifications

Power Requirements: 100/115V, 50/60Hz; 3.1 Amps at 120 Volts
230V, 50/60Hz; 1.55 Amps at 230 Volts
Mains supply voltage fluctuations are not to exceed 10 percent of the nominal

supply voltage

Pollution Degree: 2
Installation Category: II
Altitude: Up to 2000m
Ambient Temperature: 5°C to 40°C
Humidity: Maximum relative humidity of 80% for temperatures up to 31°C, decreasing

linearly to 50% maximum relative humidity at 40°C

Built-In Touch Screen Computer

Operating System: Windows® 7, 64-Bit
Connectivity Ports: 2 USB (side of system)
4 USB, VGA and Ethernet (rear of system)
Wireless Networking Capability: 802.11 b/g/n
USB Flash Drive Capacity: 2 GB (minimum)
Internal Hard Drive: 250 GB (minimum)
Software: TS2 (Touch Screen) Software

Cameras and Lenses

GelDoc-It

CCD camera with a resolution of 2.0MP (1600x1200). The GelCam 310 is equipped with a 12.5-75mm f/1.2
optical zoom lens.

ChemiDoc-It

scientific-grade monochrome CCD camera. Both cameras are Peltier cooled and offer full 16-bit file bit
depth:

All camera settings are fac tory pre-set for optimum performance when viewing gels, films or membranes
under low light level condi tions. Contact UVP Technical Support prior to making any adjustments to internal
camera settings.

TS2

: The GelDoc-It

TS2

: The ChemiDoc-It

The BioChemi 510 camera is a 2.1MP unit with Peltier cooling to 35°C below ambient. The
BioChemi 510 has a peak quantum efficiency of 50% and is capable of binning from 1x1 to 8x8.
This camera is equipped with a 12.5-75mm f/1.2 optical zoom lens.

The MegaCam 810 camera is an 8.1MP camera (3296x2472) with Peltier cooling to 35°C below
ambient. The MegaCam 810 has a peak quantum efficiency of 50% and is also capable of binning
from 1x1 to 8x8. This camera is equipped with either a 50mm f/1.2 lens or a 30mm f/1.4 lens, both
of which have a fixed focal length (non-optical zoom capable).

TS2

is equipped with the GelCam 310 camera, a scientific-grade monochrome

TS2

is equipped with either the MegaCam 810 or BioChemi™ 510

Ethidium Bromide (EtBr) Emission Filter

The ethidium bromide (50mm2) UV-blocking bandpass interference filter blocks UV and IR radiation emitted
from the transilluminator. The filter is placed in the slide-out filter wheel assembly on the side of the Imager.
The filter allows visuali zation of fluorophores from 580-630nm, targeting the ethidium bromide emission peak
of 605nm.

Additional filters are available for other specific fluorophores, including custom filters. Filters can also be
removed when imaging non-fluorescent media (inc luding chemiluminescent blots, protein gels, colony
plates, etc.) in order to produce brighter images. Contact UVP for ordering information.

GelDoc-It

Fluorescent Focus Target

TS2

and ChemiDoc-It

Darkroom

The darkroom is light tight to provide optimal imaging conditions. Darkroom features include:

• Epi (overhead) LED white light

• Roll-out transil luminato r tray

• UV-safe gel viewer window built into the darkroom door

• Five position emission filter wheel

• Brackets and built-in power ports for installing optional epi UV modules

• Brackets for installing optional high-intensity halogen or xenon BioLite™ epi light guides

• Built-in power jump er cable for transilluminator

• Power cord for optional LED White Light Plate

• UV safety interlock switch with manual override to disable UV transillumination and optional UV epi

illumination when darkroom door is opened

Transilluminator

The GelDoc-It
transilluminator models. UVP offers a variety of transilluminator configurations, including Benchtop models
with multiple wavelengths and variable intensities, as well as the highly uniform, pate nted Fi rstLi ght
transilluminator.

Note: For UV protection and to extend the life of the UV transilluminator, the system incorporates a
customizable transillum ina tor shut off timer built into the software. For additional information, refer to the
Lighting section of this manual.

TS2

and ChemiDoc-It

LCD Touch Screen

The GelDoc-It
computer. The touch screen allows the user to perform a variety of tasks, including previewing, capturing,
saving and printing images, as well as selecting preference options, without the use of an external mouse or
keyboard. The screen can be pressed by finger or with the included s tylus.

For users who prefer not to use the touch screen interface, an external keyboard and mouse are included as
standard. These components can be plugg ed into any avail a ble system USB ports.

TS2

TS2 Software

Image acquisition and hardware functions for the GelDoc-It
and ChemiDoc-It
Screen) Software interface. In addition to image preview, capture
and save functions, the TS2 Software controls camera and lens
functions (exposure, aperture, focus and zoom, where
applicable), image printing, lighting and filter selection, and other
user settings.

TS2

Imagers 6

TS2

Imagers can accommodate UVP’s Benchtop and FirstLight®

and ChemiDoc-It

TS2

Imagers are controlled by the TS2 (Touch

TS2

Imagers contain a fully integrated 15.6-inch color touch screen

TS2

TS2 Software Screen

Fluorescent Focus Target

The UVP Fluorescent Focus Target fluoresces when placed on a
UV transilluminator or when exposed to overhead UV. The Target
provides sharp fluorescent images to aid in adjusting the lens and
camera settings for ideal imaging results.

GelDoc-It

LED White Light Plate

BioLite MultiSpectral Light Source

TS2

and ChemiDoc-It

Optional Equipment

UVP offers a variety of optional equipment to support the needs of varying laboratory environments. Refer to
Replacement Parts and Accessories at the end of this manual for optional equipment part numbers.

BioLite MultiSpectral Light Source

The BioLite™ MultiSpectral Light Sour ce uti liz es high
intensity xenon or halogen lighting in conj unct io n wit h fiber
optic guides to a supply powerful, directed illumination to a
variety of fluorescent stained samples. A wide range of
excitation and emission filters enable a wide spectral range
of wavelengths, including red, green, blue and NIR.

Thermal Printer

The thermal printer provides archive quality, 256 grayscale
prints and five optional cost-effective print size s.

TS2

Imagers 7

Thermal Printer

UV Modules for Epi (Overhead) Lighting

A set of two 4-watt ultraviolet modules can be connected
inside the darkroom to provide epi UV illumination. These
modules can be switched on or off in t he Lighting menu
within the TS2 Software a nd c an also be remov ed fr om the
darkroom and used as standalone handheld lamps (as seen
in the image to the right).

LED White Light Plate

The LED White Light Plate emits high uniformity with less
than 5% coefficient of variance (CV). Plug the LED White
Light Plate directly into the power supply within the darkroom
and select “White Light Plate” under the Lighting menu
within the TS2 Software.

Epi UV Module

GelDoc-It

TS2

and ChemiDoc-It

TS2

Imagers 8

Converter Plates

An alternate to the LED White Light Plate, the UV/White
Converter Plate allows imaging of non-fluorescent sta ined

media with an ultraviolet transilluminator. The converter plate
is specially coated to convert 302nm UV to white light rather
than using a separate white light box.

The Visi-Blue™ Converter Plate (not shown) converts UV
to a safe 460-470nm wavelength desi gned for use with blue
excitation samples and SYBR Green, SYPRO Orange and
GFP stains.

UV/White Converter Plate

VisionWorks®LS Analysis Software

All TS2 systems include* a copy of VisionWorksLS Software for analysis of gels, plates and
membranes. The software features image enhancement, complete anal ysis tools and reporting
capabilities, and is ideal for users who require image analysis functions in addition to the standard
image acquisition capabilities of the TS2 software.

NOTE: Software must be installed and operated on an external computer.

*System/software configurations may vary by country. Contact UVP or authorized distributor for details.

GelDoc-It

TS2

and ChemiDoc-It

TS2

Imagers 9

Setup Instructions

Components

When unpacking the GelDoc-It

1. GelDoc-It

2. Ethidium bromide (EtBr) emission filter

3. Transilluminator

4. Power cable

5. USB flash drive (8 GB minimum)

6. Keyboard and mouse

7. VisionWorksLS Software (if included; varies by country)

8. Supporting documentation

When unpacking and setting up the darkroom, two people are required to move the darkroom.
Place the darkroom on a flat surface which can provide adequate support for up to 100 pounds.
WARNING: Do not attempt to perform any setup procedures while the system is plugged in or powered on

unless otherwise instructed.
CAUTION: Do not install the system in areas with high moisture, dust or high temperatures. Keep the

equipment away from motors or any other large magnetic equipment. This system is designed for indoor use
only.

TS2

or ChemiDoc-It

TS2

and ChemiDoc-It

TS2

system

TS2

, the following items will be included:

Connecting the Power Cables

1. Plug the main power cable into the back of the darkroom and the
other end into a surge-protected power outlet.

Note: It is recommended to leave the power swi tch on the back of
the system in the ON position except when the system will not be
used for an extended period of time (one day or longer).

Note: Do not position the system so that it is difficult to access the
power cable and operate the main power switch at the back of the unit.

2. Inside the darkroom, plac e the transilluminator on the roll-out tray. Connect the transilluminator to the
internal power jumper cable. Ensure that the green power switch on the front of the transilluminator unit
is in the ON position and that the desired wavelength or intensity is selected.

Note: For UV protection and to extend the life of the UV transilluminator, the s ystem incorporates a
customizable transilluminator shutoff timer built into the software. For additional information, refer to
Touch Screen Interface in this manua l.

3. If insta lling the LED White Light Plate, place the plate on top of the UV transilluminator and connect the
power cord coming from insi de the darkroom to the back of the Plate.

4. If insta lling epi UV modules, place the modules in the brackets located at the top of the darkroom. Plug
the modules into the outlets provided inside the darkroom. Place the power switches located on the
modules in the ON position.