UVP 81-0345-01 User Manual

TS2
GelDoc-It
®
& ChemiDoc-It
Imagers
Installation and User Instructions
TS2
®
UVP, LLC Ultra-Violet Products Ltd.
2066 W. 11th Street Unit 1, Trinity Hall Farm Estate Upland, CA 91786 Nuffield Road, Cambridge CB4 1TG UK Phone: (800) 452-6788 Phone: +44(0)1223-420022 Fax: (909) 946-3597 Fax: +44(0)1223-420561

Web Site: www.uvp.com

81-0345-01 Rev F
GelDoc-It
TS2
and ChemiDoc-It
TS2
Imagers 2

Table of Contents

Introduction .................................................................................................................................................................. 3
System Components ................................................................................................................................................... 4
Specifications ............................................................................................................................................................ 5
Built-In Touch Screen Computer ............................................................................................................................... 5
Cameras and Lenses ................................................................................................................................................ 5
Ethidium Bromide (EtBr) Emission Filter ................................................................................................................... 5
Darkroom................................................................................................................................................................... 6
Transilluminator ......................................................................................................................................................... 6
LCD Touch Screen .................................................................................................................................................... 6
TS2 Software ............................................................................................................................................................. 6
Fluorescent Focus Target ......................................................................................................................................... 6
Optional Equipment ................................................................................................................................................... 7
Setup Instructions ........................................................................................................................................................ 9
Components .............................................................................................................................................................. 9
Connecting the Power Cables ................................................................................................................................... 9
Installing Emission Filters ........................................................................................................................................ 10
Using the System ....................................................................................................................................................... 11
Powering Up the System ......................................................................................................................................... 11
Preparing the Transilluminator for Use .................................................................................................................... 11
Operating the TS2 Software Interface ..................................................................................................................... 12
Using the UV Gel Viewer Window ........................................................................................................................... 12
Image Focusing ....................................................................................................................................................... 12
Image Zooming ....................................................................................................................................................... 13
Touch Screen Interface ............................................................................................................................................. 14
Setting User Preferences ........................................................................................................................................ 14
Changing Lighting and Filter Settings ...................................................................................................................... 16
Identifying the Touch Screen Buttons and Functions .............................................................................................. 17
Using Templates ..................................................................................................................................................... 25
Connecting to a Network ......................................................................................................................................... 26
Installing Drivers or Additional Software .................................................................................................................. 26
Service Procedures .................................................................................................................................................... 27
Return Procedure .................................................................................................................................................... 27
Replacement Parts and Accessories ....................................................................................................................... 27
Troubleshooting ....................................................................................................................................................... 27
Technical Support ................................................................................................................................................... 28
GelDoc-It
TS2
and ChemiDoc-It

Introduction

The GelDoc-It fluorescent gel images.
In addition to gel documentation capabilities, the ChemiDoc-It scientific-grade CCD camera allowing users to capture and document chemiluminesc ent bl ot image s, including Western blots.
The TS2 Imagers are self-contained, light-tight imaging systems with a built-in 15.6” touch screen and computer and TS2 Software for image acquisition.
The darkroom has a UV-blocking gel viewer window, built-in overhead epi white lighting, a UV transilluminator and a five-position slide-out emission filter wheel with an ethidium bromide (EtBr) emission filter included as standard. Save images to the internal hard drive, to an external USB storage device or to a network drive for later quantitative analysis or enhancement for publication.
UVP’s VisionWorks colony plates. VisionWorksLS requires an external computer and cannot be operated directly on the TS2’s integrated computer.
*System/software configurations may vary by country. Contact UVP or authorized distributor for details.
TS2
Imagers 3
TS2
Imager is a high resolution imaging system capable of capturing and documenting
TS2
Imager is equipped with a cooled,
®
LS Analysis software is included* with all TS2 Imagers for analysis of gels, blots and
GelDoc-It
Camera and Lens
Hood
15.6” Touch Screen Computer
Computer Power
Wide Access Door
USB Access Port
Built-In Overhead
White Lights
Five Position Slide-
Wheel
UV Gel Viewer
Window
Transilluminator on
Roll-Out Tray
UV Safety Interlock
Switch & Override
Black Chemi Tray
versions only)
BioLite™ Access Port
Power Indicator
Lights
Stylus with Adhesive
Holder (Not Shown)
TS2
and ChemiDoc-It
TS2
Imagers 4

System Components

Refer to the packing slip and pictured components for specific parts and components included with the system.
Built Into Darkroom
Out Emission Filter
(ChemiDoc-It
TS2
GelDoc-It
TS2
and ChemiDoc-It
TS2
Imagers 5

Specifications

Power Requirements: 100/115V, 50/60Hz; 3.1 Amps at 120 Volts 230V, 50/60Hz; 1.55 Amps at 230 Volts Mains supply voltage fluctuations are not to exceed 10 percent of the nominal
supply voltage
Pollution Degree: 2 Installation Category: II Altitude: Up to 2000m Ambient Temperature: 5°C to 40°C Humidity: Maximum relative humidity of 80% for temperatures up to 31°C, decreasing
linearly to 50% maximum relative humidity at 40°C

Built-In Touch Screen Computer

Operating System: Windows® 7, 64-Bit Connectivity Ports: 2 USB (side of system) 4 USB, VGA and Ethernet (rear of system) Wireless Networking Capability: 802.11 b/g/n USB Flash Drive Capacity: 2 GB (minimum) Internal Hard Drive: 250 GB (minimum) Software: TS2 (Touch Screen) Software

Cameras and Lenses

GelDoc-It
CCD camera with a resolution of 2.0MP (1600x1200). The GelCam 310 is equipped with a 12.5-75mm f/1.2 optical zoom lens.
ChemiDoc-It
scientific-grade monochrome CCD camera. Both cameras are Peltier cooled and offer full 16-bit file bit depth:
All camera settings are fac tory pre-set for optimum performance when viewing gels, films or membranes under low light level condi tions. Contact UVP Technical Support prior to making any adjustments to internal camera settings.
TS2
: The GelDoc-It
TS2
: The ChemiDoc-It
The BioChemi 510 camera is a 2.1MP unit with Peltier cooling to 35°C below ambient. The BioChemi 510 has a peak quantum efficiency of 50% and is capable of binning from 1x1 to 8x8. This camera is equipped with a 12.5-75mm f/1.2 optical zoom lens.
The MegaCam 810 camera is an 8.1MP camera (3296x2472) with Peltier cooling to 35°C below ambient. The MegaCam 810 has a peak quantum efficiency of 50% and is also capable of binning from 1x1 to 8x8. This camera is equipped with either a 50mm f/1.2 lens or a 30mm f/1.4 lens, both of which have a fixed focal length (non-optical zoom capable).
TS2
is equipped with the GelCam 310 camera, a scientific-grade monochrome
TS2
is equipped with either the MegaCam 810 or BioChemi™ 510

Ethidium Bromide (EtBr) Emission Filter

The ethidium bromide (50mm2) UV-blocking bandpass interference filter blocks UV and IR radiation emitted from the transilluminator. The filter is placed in the slide-out filter wheel assembly on the side of the Imager. The filter allows visuali zation of fluorophores from 580-630nm, targeting the ethidium bromide emission peak of 605nm.
Additional filters are available for other specific fluorophores, including custom filters. Filters can also be removed when imaging non-fluorescent media (inc luding chemiluminescent blots, protein gels, colony plates, etc.) in order to produce brighter images. Contact UVP for ordering information.
GelDoc-It

Fluorescent Focus Target

TS2
and ChemiDoc-It

Darkroom

The darkroom is light tight to provide optimal imaging conditions. Darkroom features include:
Epi (overhead) LED white light
Roll-out transil luminato r tray
UV-safe gel viewer window built into the darkroom door
Five position emission filter wheel
Brackets and built-in power ports for installing optional epi UV modules
Brackets for installing optional high-intensity halogen or xenon BioLite™ epi light guides
Built-in power jump er cable for transilluminator
Power cord for optional LED White Light Plate
UV safety interlock switch with manual override to disable UV transillumination and optional UV epi
illumination when darkroom door is opened

Transilluminator

The GelDoc-It transilluminator models. UVP offers a variety of transilluminator configurations, including Benchtop models with multiple wavelengths and variable intensities, as well as the highly uniform, pate nted Fi rstLi ght transilluminator.
Note: For UV protection and to extend the life of the UV transilluminator, the system incorporates a customizable transillum ina tor shut off timer built into the software. For additional information, refer to the Lighting section of this manual.
TS2
and ChemiDoc-It

LCD Touch Screen

The GelDoc-It computer. The touch screen allows the user to perform a variety of tasks, including previewing, capturing, saving and printing images, as well as selecting preference options, without the use of an external mouse or keyboard. The screen can be pressed by finger or with the included s tylus.
For users who prefer not to use the touch screen interface, an external keyboard and mouse are included as standard. These components can be plugg ed into any avail a ble system USB ports.
TS2

TS2 Software

Image acquisition and hardware functions for the GelDoc-It and ChemiDoc-It Screen) Software interface. In addition to image preview, capture and save functions, the TS2 Software controls camera and lens functions (exposure, aperture, focus and zoom, where applicable), image printing, lighting and filter selection, and other user settings.
TS2
Imagers 6
TS2
Imagers can accommodate UVP’s Benchtop and FirstLight®
and ChemiDoc-It
TS2
Imagers are controlled by the TS2 (Touch
TS2
Imagers contain a fully integrated 15.6-inch color touch screen
TS2
TS2 Software Screen
Fluorescent Focus Target
The UVP Fluorescent Focus Target fluoresces when placed on a UV transilluminator or when exposed to overhead UV. The Target provides sharp fluorescent images to aid in adjusting the lens and camera settings for ideal imaging results.
GelDoc-It
LED White Light Plate
BioLite MultiSpectral Light Source
TS2
and ChemiDoc-It

Optional Equipment

UVP offers a variety of optional equipment to support the needs of varying laboratory environments. Refer to Replacement Parts and Accessories at the end of this manual for optional equipment part numbers.
BioLite MultiSpectral Light Source
The BioLite™ MultiSpectral Light Sour ce uti liz es high intensity xenon or halogen lighting in conj unct io n wit h fiber optic guides to a supply powerful, directed illumination to a variety of fluorescent stained samples. A wide range of excitation and emission filters enable a wide spectral range of wavelengths, including red, green, blue and NIR.
Thermal Printer
The thermal printer provides archive quality, 256 grayscale prints and five optional cost-effective print size s.
TS2
Imagers 7
Thermal Printer
UV Modules for Epi (Overhead) Lighting
A set of two 4-watt ultraviolet modules can be connected inside the darkroom to provide epi UV illumination. These modules can be switched on or off in t he Lighting menu within the TS2 Software a nd c an also be remov ed fr om the darkroom and used as standalone handheld lamps (as seen in the image to the right).
LED White Light Plate
The LED White Light Plate emits high uniformity with less than 5% coefficient of variance (CV). Plug the LED White Light Plate directly into the power supply within the darkroom and select “White Light Plate” under the Lighting menu within the TS2 Software.
Epi UV Module
GelDoc-It
TS2
and ChemiDoc-It
TS2
Imagers 8
Converter Plates
An alternate to the LED White Light Plate, the UV/White Converter Plate allows imaging of non-fluorescent sta ined
media with an ultraviolet transilluminator. The converter plate is specially coated to convert 302nm UV to white light rather than using a separate white light box.
The Visi-Blue™ Converter Plate (not shown) converts UV to a safe 460-470nm wavelength desi gned for use with blue excitation samples and SYBR Green, SYPRO Orange and GFP stains.
UV/White Converter Plate
VisionWorks®LS Analysis Software
All TS2 systems include* a copy of VisionWorksLS Software for analysis of gels, plates and membranes. The software features image enhancement, complete anal ysis tools and reporting capabilities, and is ideal for users who require image analysis functions in addition to the standard image acquisition capabilities of the TS2 software.
NOTE: Software must be installed and operated on an external computer.
*System/software configurations may vary by country. Contact UVP or authorized distributor for details.
GelDoc-It
TS2
and ChemiDoc-It
TS2
Imagers 9

Setup Instructions

Components

When unpacking the GelDoc-It
1. GelDoc-It
2. Ethidium bromide (EtBr) emission filter
3. Transilluminator
4. Power cable
5. USB flash drive (8 GB minimum)
6. Keyboard and mouse
7. VisionWorksLS Software (if included; varies by country)
8. Supporting documentation
When unpacking and setting up the darkroom, two people are required to move the darkroom. Place the darkroom on a flat surface which can provide adequate support for up to 100 pounds. WARNING: Do not attempt to perform any setup procedures while the system is plugged in or powered on
unless otherwise instructed. CAUTION: Do not install the system in areas with high moisture, dust or high temperatures. Keep the
equipment away from motors or any other large magnetic equipment. This system is designed for indoor use only.
TS2
or ChemiDoc-It
TS2
and ChemiDoc-It
TS2
system
TS2
, the following items will be included:

Connecting the Power Cables

1. Plug the main power cable into the back of the darkroom and the other end into a surge-protected power outlet.
Note: It is recommended to leave the power swi tch on the back of the system in the ON position except when the system will not be used for an extended period of time (one day or longer).
Note: Do not position the system so that it is difficult to access the power cable and operate the main power switch at the back of the unit.
2. Inside the darkroom, plac e the transilluminator on the roll-out tray. Connect the transilluminator to the internal power jumper cable. Ensure that the green power switch on the front of the transilluminator unit is in the ON position and that the desired wavelength or intensity is selected.
Note: For UV protection and to extend the life of the UV transilluminator, the s ystem incorporates a customizable transilluminator shutoff timer built into the software. For additional information, refer to Touch Screen Interface in this manua l.
3. If insta lling the LED White Light Plate, place the plate on top of the UV transilluminator and connect the power cord coming from insi de the darkroom to the back of the Plate.
4. If insta lling epi UV modules, place the modules in the brackets located at the top of the darkroom. Plug the modules into the outlets provided inside the darkroom. Place the power switches located on the modules in the ON position.
GelDoc-It
TS2
and ChemiDoc-It
TS2
Imagers 10

Installing Emission Filters

To install the 50mm2 ethidium bromide (EtBr) filter and any other emission filters:
1. Carefully remove the filter from the protective plastic case,
holding the filter at the edges to prevent placing fingerprints on the glass surface.
2. The filter wheel is located on the user’s right side of the
darkroom. Press in on the slide-out filter wheel door and the door will pop out slightly. Pull the filter wheel assembly out until it stops.
3. Manually rotate the filter wheel to the desired position. Place the ethidium bromide filter in Position
#1. NOTE: Before placing the filter in the filter wheel, ensure that the text on the edge of the filter is
positioned so it is right side up when facing the installer.
4. Note the position of all installed filters for entry into the Filters menu in the TS2 Software.
5. Once all filters have been loaded, slide the filter wheel assembly back into the system until it locks
into place. NOTE: In order for the system to accurately determine the position of the emission filter wheel,
press the Reset Wheel button in the Filters menu after the wheel has been moved by hand to load, change or remove filters. This will recalibrate the emission filter wheel alignment. Refer to the Filters section in this manual for more information.
Additional and replacement emission filters are available through UVP. Refer to the Replacement Parts and Accessories section of this manual for ordering information.
GelDoc-It
Power Button
Power Indicator
Lights
TS2
and ChemiDoc-It
TS2
Imagers 11

Using the System

Powering Up the System

Set the black power switch on the back of the s ystem to the ON (I) position. Once plugged in to a wall outlet and the black power switch on the back of the system is placed in the ON (I) position, the GelDoc-It
Press the POWER button on the front of the system to power on the internal computer/touch screen. Once the comp uter completely boots, the TS2 software will load automatically.
The POWER button will illuminate blue when the internal computer and touch screen are on. (Note: The black main power switch on the back of the unit must be in the ON (I) position in order for the internal computer and touch screen to function.)
The Power Indicator Lights on the front of the unit indicate when the system’s main power and ultraviolet illumination are on. Main Power will illuminate green when the black main power switch on the back of the unit is in the ON (I) position (this does not indicate that the internal computer or touch screen are on). Ultraviolet On will illuminate red when ultraviolet illumination is active within the darkroom.
Note: It is recommended to leave the power swi tch on the back of the system in the ON position except when the system will not be used for an extended period of time (one day or longer).

Preparing the Transilluminator for Use

TS2
and ChemiDoc-It
TS2
systems are always powered on.
1. Open the darkroom door and turn on the main power switch located on the front of the transil luminator. Place this switch in the ON (I) position. (Note: It is recommended to always leave this power s witch on the ON (I) position.)
2. Use the rotary knob on the front of the transi lluminator to select from the available lighting wavelengths or intensities (transilluminator settings vary by model).
Note: The GelDoc-It UV when the darkroom door is open. This switch is located on the upper right corner of the darkroom door opening and is only accessible when the door is open. To override the UV interlock, pull out on the switch. The switch will automatically reset after the darkroom door is closed.
Caution: UV Transilluminators are powerful sources of UV radiation which will cause damage to unprotected eyes and skin. Before overriding the UV interlock, be sure all personnel in the area are properly protected, including proctective eyewear and clothing.
Refer to the transilluminator manual for additional instructions on using the transilluminator.
TS2
and ChemiDoc-It
TS2
systems integrate a UV interlock switch which will inactivate all
GelDoc-It
TS2
and ChemiDoc-It
TS2
Imagers 12

Operating the TS2 Software Interface

Upon startup, the internal computer will proceed throu gh t he boot-up process. When complete, the Windows desktop will appear. The TS2 Software screen, similar to the one below, will open automatically shortly thereafter.
To exit the TS2 Software interface, press either the close (X) or minimize (_) buttons at the top right corner of the software (see red circle above). To power down the system, press the POWER button located on the front of the TS2. Once the POWER button light is extinguish ed, set the power switch on the back of the system to the OFF (O) position if the system will not be used for an extended period of time.
Refer to the Touch Screen Interface section in this manual for further instruction s on using the softw are.

Using the UV Gel Viewer Window

The UV Gel Viewer Window, built into the darkroom door, allows users to view the interior of the darkroom without opening the main door.
To open the Window, press firmly on the top of the Window cover to release the pressure-sensitive cla sp and open the viewer. The Window glass is UV blocking while providing a clear view to the transilluminator surface for sample viewing.
NOTE: Close the UV Gel Viewer Window prior to capturing a light-sensitive image such as a chemiluminescent blot.

Image Focusing

Prior to capturing an image, prepare the image focus as follows:
1. Remove the blue protective film from the Fluorescent Focus Target (see the “Fluorescent Focus
Target” section of this manual for more information).
2. Turn on the transilluminator and place the Target on the transilluminator surface.
NOTE: The darkroom has a UV safety switch that turns off the transilluminato r and optional epi UV modules when the door is open.
3. Using the TS2 Software, press Start Preview to begin viewing the sample within the system.
Adjust the exposure, focus, zoom (if applicable) and aperture controls using the Focus, Zoom and Aperture menus in the TS2 Software screen until an ideal image is visible.
Refer to Touch Screen Interface in this manual for further instructions on using the software.
GelDoc-It
TS2
and ChemiDoc-It

Image Zooming

Both the GelDoc-It are equipped with optical zoom lenses, meaning that the system uses the lens’ optics to make the sample appear closer/larger on the screen. Optical zoom is adjusted using the vertical slider in the Zoom menu on the TS2 Software screen.
The ChemiDoc-It zoom is not available. However, given the camera’s high megapixel resolution, digital zooming can be used to move in closer on the image. Digital zoom enlarges a portion of the image, simulating optical zoom. Thus, the camera crops a portion of the image and enlarges the cropped portion to fill the imaging area on the screen.
To use digital zoom functionality:
1. With a preview or captured image on the screen, use the “+” and “–” buttons located on the right
side of the image.
2. Tap and drag to move around on the zoomed-in image.
Notes regarding digital zoom operati on:
The software’s digital zoom feature utilizes WYSIWYG, or “what you see is what you get,” mean ing
that a zoomed preview image will result in a zoomed cap ture image.
A zoo med capture image will save and print as shown on the screen (WYSIWYG).
Note: Digital zoom is available on all TS2 systems, regardless of camera configuration. However, it is only recommended to use digital zoom with the MegaCam 810 due to the camera’s high megapix el resolution.
Refer to the Zoom section of Touch Screen Interface in this manual for further information.
TS2
Imagers 13
TS2
with the GelCam 310 camera and the ChiemiD oc-It
TS2
with the MegaCam 810 cam era utilizes a fixed focal length lens, meaning that optical
TS2
with the BioChemi 510 camera
GelDoc-It
TS2
and ChemiDoc-It
TS2
Imagers 14

Touch Screen Interface

Setting User Preferences

The Settings portion of the TS2 Software allows the user to select preferences which are normally set once and rarely changed. Such settings include image save format and image save location. Access the user preferences by pressing the Settings button in the upper-right corner of the main TS2 screen and select from the following tabs:
General Settings
Post-Processing
Saving
The following pop-up screen will appear:
General Settings Tab
Language: Use the drop-down arrow to select the des i red language for the TS2 software interface. Multiple language options are available including English (US), Chinese (simplified), Turkish, Japanese, Korean, Russian, Portuguese, Spanish and German.
Save Template: Select from Ask, Always or Never to choose whether the software should ask to save any changes to a template, always save the changes automatically without asking, or never save the changes automatically.
Post-Processing Tab
Auto Rotate: Set Auto Rotate to ON to automatically rotate the image to the desired degree upon image capture. Note: “Auto Rotate” must be set to ON in order to rotate images during image captur e,
as images cannot be rotated after capture using the TS2 software.
To select the degree of image rotation, press the down arrow from the drop-down menu and choose to rotate the image 90 degrees clockwise, 90 degre es counterclockwise, or 180 degrees (upside down).
Note: “Auto Rotate” mus t first be turned ON before selecting a rotation setting.
GelDoc-It
TS2
and ChemiDoc-It
Auto Invert Image: Set Auto Invert Image to ON to automatically invert the image upon image capture.
Note: “Auto Invert Image” must be set to ON in order to invert images during image capture; captured images can also be inverted in the Gallery view.
Noise Subtraction: Set Noise Subtraction to ON to reduce the amount of background and ambient (“white”) noise within the image. In most circumstan ce s, this setti ng sho uld be left ON. Note: “Noise
Subtraction” must be set to ON in order to subtract noise from images during image capture, as noise cannot be subtracted from images after capture using the TS2 software.
TS2
Imagers 15
Saving Tab
Save Format: Press the down arrow to select the desired file save format from the drop-down menu. Save images in JPEG, TIFF, BMP or PNG file formats.
From the drop-down menu to the right of the chosen file format, choos e Save Selected Format to save the image only in the chosen format. Or, select Save Selected and Original Formats to save in both the selected format as well as in an uncompressed TIFF format (note: two separate files will be saved using this method).
Note: If the TIFF file format is selected and Save Selected and Original Formats is also select ed, both compressed and uncompres sed TIFF files will be saved.
Save Images To: Select the location where images are to be saved. The black dot within the radio button indicates which selection is activated.
1. Select USB to save the file to the USB drive if one is currently inserted. If a USB device is not
present, the user will be notified that a USB drive is not present when attempting to save an image.
2. Select Prompt for Location to prompt the user to select a file save location when attempting to
save an image. This setting will also allow the user to save using a custom file name.
3. Choose Select Folder to define where the file will be saved when attempting to save an image.
Select from any local or network drive by pressing the folder icon to the right of the file path display.
Note: The Select Folder radio icon must first be selected prior to defining the save location.
Auto Save After Capture: Set Auto Save After Capture to ON to automatically save the im age after capture. The image will automatically save to the location selected in Save Images To and in the format selected in Save Format, as described above.
NOTE: If the Auto Save After Capture function is set to OFF, press the Save button in the Gallery view to manually save an image.
Auto Print After Capture: Select ON to automatically send the captured image to the default printer after an image is captured. If a default printer is not installed, the Windows “Printers and Faxes” dialog will automatically appear Faxes” dialog box and press OK on the dialog box to print the image.
after the image is captured. Select the desired printer in the “Printers and
Accept or Cancel Settings
Once all Preferences settings have been made, press the Checkmark button at the bottom of the Preferences screen to save all preferences and go back to the main TS2 screen. Or, press the “X” button to go back to the main TS2 screen without saving changes.
GelDoc-It
TS2
and ChemiDoc-It
TS2
Imagers 16

Changing Lighting and Filter Settings

Before previewing or capturing images, choose the appropriate lighting and emission filter settings.
Lighting
Press the Lighting button on the main TS2 screen to access the lighting selection menu. The following options will appear:
Epi Illumination: Press the desired radio button to select from the following epi illumination sources: White, Accessory or Off.
1. White lighting produces a glow of white light from overhead.
2. Accessory lighting is only functional when optional epi UV modules are installed in the system
darkroom.
3. Off will disable all overhead lighting.
Transillumination: Press the desired radio button to select from the following transillumination sources: UV, White Light Plate or Off.
1. UV will activate the ultraviolet transilluminator within the system darkroom, providing ultraviolet
base lighting of variable wavelengths or intensities (depending upon system configuration).
2. White Light Plate base lighting is only functional when the optional LED white light plate is
installed in the darkroom.
3. Off will disable all transillumination. Inactivity Light Shutoff: The Inactivity Light Shutoff will automatically turn off the transilluminator within
the darkroom after a selected period of time. From the drop down menu, select from 1, 5, 10, 15, 20 or 25 minutes of inactivity time prior to shutoff. To re-enable lighting after the inactivity light shutoff period, access the Lighting button from the main screen and turn the lighting on again.
Filters
Press the Filters button on the main TS2 screen to access the filter selection menu. Filter Selection: Select the desired emission filter by pressing the appropriate radio button. Select from
one of five filter positions on the built-in filter wheel. Reset Wheel: Press the Reset Wheel button to recalibrate the emission filter wheel alignment after the
wheel has been moved by hand to load, change or remove filters. NOTE: If Reset Wheel is not pressed after the filter wheel has been manually rotated, the system will not be able to accurately determine the position of the emission filter wheel.
GelDoc-It
TS2 Button
Function
Throughout the TS2 interface, the “Off” and “On” Sliders
Access user preferences by pressing the Settings button
Press the Templates button to access the list of templates.
To view a preview of the image prior to capturing, press
To capture an image with user-defined settings, press the
NOTE: When using the Start Capture button, if the Auto
TS2
and ChemiDoc-It
Edit: To change a filter name, press the desired radio button to select the emission filter and then press the Edit button. A screen will appear allowing the user to type in a new filter name. Press Checkmark button to accept the new name or the “X” button to cancel the filter name change.
TS2
Imagers 17

Identifying the Touch Screen Buttons and Functions

Using the TS2 System’s built-in touch screen monitor allows for convenient selection of all system functions including image capture, save and print. Following is a list of buttons on the touch screen and their individual functions.
are used to turn settings either off or on. To toggle
“Off” and “On” Sliders
“Acquisition” Tab
between off and on, tap the Slider; the Slider will autmatically move between the “X” (off) and the checkmark (on).
To access system acqu i si t io n sett i ngs, press to select the Acquisition tab.
the
Settings
Templates
Maximize Minimize
in the upper-right corner of the main TS2 screen. The Settings screen allows the user to select settings whic h are normally chosen once and rarely changed, such as interface language and image save format.
See the Setting User Preferences section of this manual for further information.
Press again to close the list of templates. For more information on templates, see the Using
Templates section of this manual.
the Start Preview button. This function is active when the button’s text and pictogram
are shown in purple and read “Stop Preview”. When active, press the button again to deactivate live preview.
When an image preview is open, press the Maximize button to show the image in full-screen mode.
Press the Minimize button to close full-screen.
Start Capture button. For longer exposures, the amount of time remaining for the capture to complete will appear to the right of the “Start Capt ur e” but ton .
This function is active when the button reads “Stop Capture” in purple lettering.
GelDoc-It
Save After Capture function is enabled in Preferences,
Use the slider bar to adjust various settings, including
Press the Focus button to access lens focus adjustment.
Press the Zoom button to access lens optical zoom
Press the Aperture button to access lens aperture
Press the Capture Mode button to access exposure time
Marker
TS2
and ChemiDoc-It
TS2
Imagers 18
Slider Bar
Focus
the image will automatically be saved to the preset location (see “Saving Tab” under Setting User Preferences in this manual).
Exposure, Aperture, Histogram, Focus and Zoom (if applicable). To adjust the settings, do one of the following:
Press and drag the marker (small triangle) to the desired position;
Touch anywhere along the gray slider bar and the marker will automatically “snap” to that position (not applicable to Histogram);
or
Press the “+” and “–” buttons to make fine adjustments to the settings (not applicable to Histogram).
Use the vertical slider bar and/or the “+” and “–” buttons below the slider to make adjustments to this setting.
The current focus setting will be indicated above the slider bar.
Zoom
Digital Zoom Buttons
Aperture
Capture Mode
adjustment (if available). Use the vertical slider bar and/or the “+” and “–” buttons below the slider to make adjustments to this setting. Note that the higher the zoom percentage is, the larger the image will appear.
The current zoom setting will be indicated above the slider
bar. NOTE: Optical zoom functionality is not available on all
systems. To digitally zoom in on previewed or captured images, use the Digital Zoom Buttons (“+” and “–”) located to the right of the active image.
adjustment. Use the vertical slider bar and/or the “+” and “–” buttons to make adjustments to this setting. Note that the lower the aperture setting (the lower the f-number), the more light will pass through to the camera’s sensor.
The current aperture setting will be indicated above the slider bar.
adjustment for live image preview and capture, as well as to select modes for image capture.
Use the vertical slider bars and/or the “+” and “– buttons to make adjustments to the desired portion of the exposure time (min, sec, ms or microseconds).
To adjust capture modes, select the desired mode from the drop-down menu. Capture modes include:
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Manual Exposure: Captures one (or m ore) images with
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a pre-selected exposure time.
Auto Exposure: Captures an image with an ideal exposure time determined automatically by the system prior to image capture. (NOTE: When using the Auto Exposure function, any exposure time settings made by the user prior to image capture will be changed.)
After selecting Auto Exposure, select from one of the following image acquisition settings:
1. Best (Longer Exposure) expos es the image to the
maximum value of the histogram (65,000 gray levels).
2. Better exposes to fill the histogram 50% so the
brightest portion of the image is at 32,000 gray levels.
3. Good exposes to fill the histogram to 25% or 16,000
gray levels.
4. Minimum (Fast Exposure) exposes to fill the
histogram to 10% over background. Minim um and Good settings are particularly useful for chemiluminescent imaging ap plicat i ons and all ow for quicker image capture overall.
Sequential – Constant Time: Select the num ber of frames to be captured as well as the total image capture time, and the software will automatically capture the desired number of frames with evenly divided exposure times.
Sequential – Variable Time: Captures sequential images with user-selectable exposure times per image. For example, capture one image at 300ms ex posure, immediately followed by another image captured at 200ms exposure, and so forth.
Click “+” to add a new interval. Tap to edit each time block (Min, Sec, Ms and Micro) as needed, then use the on-screen keyboard to make edits. Or, use the dropdown menu to select from several pre-defined interval sets for specific applications.
Image Integration – Continuous: Captures multiple images at a desired exposure time and continual ly “stacks” the images, compensating for low-light limitations. Image Integration - Continuous will continue image acquisition and “stacking” until the user stops the image capture.
Image Integration – Automatic: Similar to Image Integration - Continuous, except the software
automatically stops image capture and “stacking” when the ideal level of saturation has been achieved.
Select the Number of Frames to be captured during Manual Exposure and Sequential – Constant Time image capture modes. Use the “+” and “–” buttons to select the desired number of frames.
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Press the Histogram button to access image histogram
Press the Binning/Interpolation button to access image
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Histogram
Binning/Interpolation
adjustment. Use the pointers on the vertical slider bar to make adjustments to this setting. Slide the top marker down to darken the image, or slide the bottom marker up to lighten the image.
Push the “Reset” button to reset the markers to the top and bottom of the slider bar, showing the image’s full histogram range.
Turn on Auto Adjust to automatically adjust the image histogram for ideal imaging results.
Note: When a color image is present, the histogram menu will show three separate slider bars, allowing histogram settings for red, green and blue channels.
NOTE: Histogram settings also apply to the Gallery view, discussed later in this manual.
binning and interpolation adjustment options. Binning is the process of combining multiple im age pixels
into one larger pixel. For example, 2x2 binning combines a two-by-two area of pixels (four pixels all together) into one large pixel, 4x4 binning combines a four-by-four area of pixels (sixteen pixel s al l together) into one large pixel, and so forth. This process shortens the image preview and capture process when increased sensitivity is needed, and also aids the camera’s sensor in capturing and display i ng more image signal in low-light situations (such as in chemiluminescence applications). The disadvantage of binning is an overall reduction of resolution, as the effective image pixel area is exponentially reduced as binning is increased.
Select a higher Preview Binning to increase the preview frame rate and make sample previewing faster. Preview binning will not affect the captured image. (Note: Preview binning is only available on chemi-capable systems.)
Select a higher Capture Binning to decrease image capture time or to acquire more image signal in the same amount of time, especially in low light situations. Note that increased capture binning will decrease the overall pixel resolution of the captured image. (Note: Capture binning is set to 1x1, thus no binning, by default.)
Depending upon system model, image Interpolation settings may also be found in the Capture Binning dropdown menu. By utilizing advanced algorithms integrated into the TS2 software, Interpolation effectively increases an image’s resolution bey ond the camera ’s native resolution. To activ ate i mage interpolation, select the desired interpolated resolution from the Capture Binning dropdown menu.
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Turn on Saturation Warning to provide a bright yellow or
Press the Template Action button to select which action
After all template settings have been made, press the
To access the photo gallery, press to select the Gallery
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Saturation Warning
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red image overlay on oversaturated areas of the image during Live Preview. Yellow indicates mild overexposure while red indicates extreme overexposure.
Action
To capture an ideally exposed image, decrease the aperture or exposure time until the yellow or red overlay disappears.
To activate saturation warning, press the Saturation Warning slider until the check mark appears.
the TS2 Imager will take when a template is selected. Available actions include:
Start Capture: When a template includes this Action,
all system settings will be adjusted according to the template and then the image will automatically be captured.
Start Preview: When a template includes this Action,
all system settings will be adjusted according to the template and an image preview will automati cally be shown.
Do Nothing: When a template includes this Action, all
system settings will be adjusted according to the template; no image preview or capture will occur.
Done and Save
“Gallery” Tab
Open Image
Done (checkmark) button. The n, to save the selected settings as a template for future use, press the Save (disk) icon.
A window will appear prompting the user to enter a template name using the on-screen keyboard. After the name has been entered, press the Checkmark button to accept the name or the “X” button to c ancel.
tab. Once active, select the desired image from the top of the
Gallery screen.
To open a previously-saved image, press the Open Image button. Pressing this button will open the Windows file/folder navigation screen. Select the desired file and press Open.
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Use the Gallery Navigation buttons to navigate through
Press the “i(Information) button to view information
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Gallery Navigation
Maximize Minimize
Close
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the image gallery. When multiple “pages” of images appear in the gallery:
Press Previous to go to the previous page.
Press Next to go to the next page.
Press Last to go to the newest picture in the Gallery.
Press Active to go to the active image shown on the
main image screen.
When an image is open in the Gallery, press the Maximize button to show the image in full-screen mode.
Press the Minimize button to close full-screen mod e.
Press the Close button to the right of the image to close the active image. If the image is unsaved, the user will be prompted to save the image before closing.
Information
Digital Zoom Buttons
pertaining to the open image. Information includes exposure time, illuminati on, fo cus and apert ur e sett ing s. Press the “i” button again to close the image information screen.
NOTE: Image information is only available for images captured using the TS2 Imager.
Use the “+” and “–” buttons located to the right of the active image to digitally zoom in or out on Gallery images.
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Press the Save Burned button to save the image with all
To add a date stamp to the c aptured image, press the
Press the Pseudocolor button to access a variety of
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Save Burned
Save
modifications (such as time stamp and histogram modifications) embedded in the image. Or, press the Save button to save the raw image without any modific ations embedded. Images will be saved to the location specified in Preferences.
The file name is automatically assigned by the software as mmmddyyyyhhmmss, with “mmmddyyyy” being the date of image capture and “hhmmss” being the time of image capture.
NOTE: To manually change the file name when saving an image, select Prompt for Location under the Saving tab in Settings prior to saving. Then, each time the a save button is pressed, the user will be prompted to select a file save location and can enter a custom file name.
NOTE: If Save Selected and Original Formats is selected in the Settings menu (described earlier in this manual) and the Save button is pressed, both the selected and original formats will be saved. However, if the Save Burned button is pressed, only the s elected format will be saved.
Print
Histogram
Time Stamp
Pseudocolor and Invert
Press the Print button to print the current image on the default printer. If a default printer is not installed, pressing the Print button will place the print request in queue.
See the Histogram section in the Acquisition portion of this manual for information on using the histogram in Gallery view.
Time Stamp button, then press the slider until the check mark appears. This will add UVP#####mmm/dd/yyyy
hh:mm:ss to the bottom right corner of the image. NOTE: The time stamp is not saved to the image unless
Save Burned is selected (as described earlier in this
manual).
Pseudocolor options for captured images. Pseudocolor options include in vivo, oversaturation (shows yellow to indicate mild overexposure and red to indicate extreme overexposure), yellow, red, green and blue. Press appropriate radio button to select the desired pseudocolor.
Press the Invert button to access image inversion selection. Under Invert, touch the slider until the che ck mark appears to activate image inversion.
NOTE: Pseudocolors and image inversion are not saved to the image unless Save Burned is selected (as described earlier in this manual).
the
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To access image compositing tools, press to select the
The Fluorescent Compositing Tool is used to merge
The Merge Compositing Tool is used to merge up to
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“Compositing” Tab
Fluorescent Compositing Tool
Compositing tab. Copositing tools include Fluorescent, Merge and
Chemiluminescent. Press to select the desired compositing tool once the Compositing tab has been selected.
various fluorescently-color ed c hanne ls into one image . To use this tool, first open two or three images in the gray
area (gallery bar) below the “Compositing” tab. Drag the base/primary image from the gallery bar to the “Drag image here” Base Image area. Then, drag one or two overlay images to the second and third “Drag image here” boxes.
The base and overlay images’ histogram, pseudocolor and inversion attributes can be adjusted to create the ideal composited image. To do so, select the desired image to be adjusted by pressing it. A black outline will surround the selected image. Then, use the Histogram, Pseudocolor and Invert tools on the left side of the screen to modify the image(s) and Preview composited image as desired.
Use the percentage tool to the right of the overlay image(s) to determine the intensity of the overlay image(s) in the final composited image. Note that the percentage shown pertains to the saturation levels in the overlay image(s). Therefore, as higher perc entages are selected, only pixels with higher saturation levels will be shown in the final composited image. For example, if 65% is selec ted, only pixels with over 65% saturation in the overlay image will be shown in the final composited image.
Once the ideal composited image has been created, press the Checkmark button below t he Preview composited image to complete fluorescent compos itin g.
Merge Compositing Tool
three different images, pulling a specific color channel (red, green or blue) from each image. This tool is useful for multiplexing applications .
The Merge Compositing Tool consists of three gray rectangles where images can be placed (each rectangle states “Drag image here”). The color channel to be pulled from the image in each box is shown in the colored bar below the gray box (red on top, green in the middle, and blue on the bottom).
To use this tool, first open two or three images in the gray area (gallery bar) below the “Compositing” tab. Depending upon the desired color to be compo sit ed, dr ag an image from the gallery bar to the “Drag image here” area.
Each image’s histogram, pseudocolor and inversion attributes can be adjusted to create the ideal composited image. To do so, select the desired image to be adjusted by pressing it. A black outline will surround the selected image. Then, use the Histogram, Pseudocolor and Invert tools on the left side of the screen to modify the image(s) and the Preview composited image as desired.
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Once the ideal composited image has been created, press
The Chemiluminescent Compositing Tool is used to
Press the Minimize (“_”) button in the upper-right corner of
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Chemiluminescent Compositing
Minimize and Close
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Tool
the Checkmark button below t he Preview composited image to complete fluorescent compos itin g.
merge various image channels for chemiluminescent imaging.
This tool functions exactly the same as the Fluorescent Compositing Tool described ear li er, except that the middle overlay image will always automatically be inverted. This is ideal for chemilum inescent white light overlays.
For more information on using this tool, see the Fluorescent Compositing Tool as described earlier in this manual.
the screen to minimize the TS2 Software. Press the Close (“X”) button in the upper-right corner of
the screen to close the TS2 Software. If any unsaved images are open prior to cl osing the TS2 software, the user will be prompted to choose one of the following:
1. Save the current image
2. Not save the current image
3. Cancel closing the software
4. Save none of the images
5. Save all images The user will also be prompted to Save Selected
Format or Save Selected and Original Formats. See the “Preferences” section of this manual for more information.

Using Templates

The TS2 Imagers are capable of utilizing templates to recall pre-saved systems settings for repeat experiments. An unlimited number of templates can be saved in the system, with up to five quick-access templates available at the top of the main system screen for easy access.
To create a template:
1. Set the various system settings (lens settings, lighting, filters, etc.) as desired following the instructions shown in the Identifying the Touch Screen Buttons and Functions section of this manual.
2. Once all desired settings have been selected, press Done at the bottom left of the screen. A summary of all settings will be shown. Then, press Save to save the template:
a. For new templates, a popup will appear requesting for the template to be assigned a
name.
b. For existing templates, the keyboard will appear wi th the template name shown. The user
can then accept the current name by pressing the Checkmark button, enter a new template name then press the Checkmark button, or press the “X” button to cancel saving.
3. To access saved templates, press the Templates button as shown:
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4. To select the quick-access templates to be shown at the top of the TS2 screen, press the Templates button until the list of saved templates is shown. Then, drag the template to the desired quick-access position.
To run a template, either:
1. Select the desired template from the Templates menu by pressing the gray check box icon to the right of the template name, or
2. Select the template from the quick-access area.
Once the template is selected, all template settings and actions will automatically be performed on the system.
A template is active when the template button shows white letters on a purple background. A template is inactive when black letters are shown on a gray background.
To edit a template name, press the gray pencil icon to the right of the template name in the list of saved templates. The template settings will be shown. Press the pencil icon in the upper-left corner of the screen to the left of the template name. An on-screen keyboard will appear. Use the keyboard to enter the desired template name. Press the Checkmark button to accept the revised name, or press the “X” button to cancel.
To delete a template, press the gray trash can icon to the right of the template name. To disregard a template and enter settings and actions manually, press the
Manual Input button located at the top right of the software:

Connecting to a Network

The TS2 Imagers have built-in network capability, both wired and wireless. While it is fairly simple to connect the system to a network, it is highly recommend to obtain assistance from a network administrator to ensure that the process is completed properly.
Follow Microsoft or local standard network protocols for network configuration. To minimize the TS2 Software interface and access Microsoft Windows for network configuration, press the Minimize (“_”) button in the upper-right corner of the software.

Installing Drivers or Additional Software

In the event that additional drivers or softwar e must be installed on the system, exit th e TS2 Software interface and access Microsoft Windows by pressing the Close (“X”) button located in the upper-right corner of the main TS2 screen.
To install drivers or additional software, copy the software to an external storage device, open Windows Explorer, navigate to the appropriate folder and run the desired program.
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Service Procedures

Return Procedure

A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service before returning any product.

Replacement Parts and Accessories

To order accessories or replacement parts for the GelDoc-It offices.
Part Description Part Number
Epi UV Modules:
UV Module, 254/365nm, 4 watt (115V) 95-0021-12 (Qty. 2 Recommended) UV Module, 254/365nm, 4 watt (230V) 95-0021-10 (Qty. 2 Recommended)
Fuses:
Fuse, 3.15A (for Darkroom) 56-0022-04 (Qty. 2 Required) Fuse, 2A (for Transilluminators) 56-0002-01 (Qty. 2 Required)
Emission Filters:
Filter, Ethidium Bromide, 50mm Square 38-0220-01 Filter, SYBR Green, 50mm Square 38-0219-01 Filter, SYBR Gold, 50mm Square 38-0221-01
Transillumination Accessories:
LED White Light Plate 95-0476-01 White Light Converter Plate, 21x26cm 38-0191-01 Visi-Blue Converter Plate, 21x26cm 38-0200-01
Gel Accessories:
Gel-Cutter 85-0002-01 Gel-Ruler 85-0003-01 Gel-Scooper 85-0006-01 Gel-Tray, small 85-0007-01 Gel-Sentry DNA Preparation Plate 97-0076-01 Fluorescent Standard Step Tablet 33-0014-02
Protective Equipment:
Spectacles, UV Blocking (UVC-303) 98-0002-01 Goggles, UV Blocking (UVC-503) 98-0002-02 Faceshield, UV Blocking (UVC-803) 98-0002-04

Troubleshooting

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and ChemiDoc-It
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Imagers, contact UVP’s
No Power to the Darkroom or Transilluminator
1. Recheck the main power cord connection to the GelDo c-
TS2
/ChemiDoc-It
It between the darkroom and transilluminator, LED White Light Plate or optional epi UV modules.
2. Check the fuses located at the back of the unit next to the power port. A small flat-head screwdriver or similar tool is required. Push the bottom t ab of the fuseho lder up until the bottom of the fuseholder pops out. Then, pus h the top tab down until the top pops out. The entire fuseholder can now be removed.
TS2
darkroom as well as the power cables
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and ChemiDoc-It
Inspect the thin wire within each glass fuse to see if there is a break in the wire. If so, replace the fuse(s). If fuses are blowing repeatedly, contact UVP Technical Support for additional troubleshooting.
Transilluminator Will Not Turn On
1. In addition to turning on “UV transillumination” in the Lighting menu within the TS2 Software, the transilluminator itself has a power switc h. Make sure that the green transilluminator power switch, located on the front of the transilluminator, is in the ON position.
2. For UV protection and to extend the life of the UV transilluminator, the system incorporates a customizable transilluminator shutoff timer built into the software. For additional information, refer to the Lighting section of this manual.
Error Messages Appear on the Screen
1. An error message that is related to the TS2 software interface or Microsoft Windows may appear on the screen. If the message is related to Mic rosoft Windows, such as a reminder to activate or update the copy of Windows, please contact your system administrator for assistance.
2. If an error message appears repeatedly and your system administrator does not recognize it as a Microsoft Windows error, contact UVP Technical Support for further assistance.
Care and Cleaning
Use only mild soap or detergent solution for cleaning the GelDoc-It petroleum-based cleaners for the cabinet. Ensure that the system is turned OFF and unplugged duri ng cleaning.
When cleaning the transilluminator surface, use a damp soft cloth or sponge. Never use abrasive cleaners which can damage the UV filter surface.

Technical Support

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TS2
/ChemiDoc-It
TS2
. Do NOT use oil- or
UVP offers free lifetime technical support on all of its products and software. Should you have any questions regarding the product’s use, operation or repair, contact UVP’s offices at the locations below, or visit www.uvp.com.
If you are in North America, South
America, East Asia or Australia:
Call (800) 452-6788 or (909) 946-
3197, and ask for Customer Service
during regular business days, between
7:00 am and 5:00 pm, PST.
E-mail your message to:
info@uvp.com
Fax Customer Service, and send it to
(909) 946-3597
Write to: UVP, LLC 2066 W. 11th
Street, Upland, CA 91786 USA
If you are in Europe, Africa, the
Middle East of Western Asia:
Call +44(0) 1223-420022, and ask for
Customer Service during regular
business days between 9:00 am and
5:30 pm.
E-mail your message to: uvp@uvp.co.uk
Fax Customer Service, and send it to:
+44(0) 1223-420561
Write to: Ultra-Violet Products Ltd
Unit 1, Trinity Hall Farm Estate, Nuffield
Road, Cambridge CB4 1TG UK
GelDoc-It, ChemiDoc-It, Vision Works and Fir st Light are r egi st ered t rademarks of U VP , LLC. BioChemi, BioLite and Visi-Blue are trademarks of UVP, LLC. All other trademarks are the property of their respectiv e owners.
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