Taylor-Wharton LABS Series User Manual

LABS

(Laboratory Archival Biological Storage)

OPERATION & MAINTENANCE INSTRUCTIONS

CRYOSTORAGE SYSTEM

CAUTION - SAFETY FIRST!

• REVIEW AND UNDERSTAND ALL SAFETY PROCEDURES IN THE “HANDLE WITH CARE” BOOKLET FORM # TW-10 P/N 7950-8052 BEFORE ATTEMPTING TO INSTALL, OPERATE OR PERFORM MAINTENANCE ON THIS CRYOSTORAGE SYSTEM.

• DO NOT ATTEMPT TO USE OR MAINTAIN THIS UNIT UNTIL YOU READ AND UNDERSTAND THESE INSTRUCTIONS.

• DO NOT PERMIT UNTRAINED PERSONS TO USE OR MAINTAIN THIS UNIT.

• IF YOU DO NOT FULLY UNDERSTAND THESE INSTRUCTIONS, CONTACT YOUR SUPPLIER FOR FURTHER INFORMATION.

• BEFORE ATTEMPTING TO OPERATE THIS EQUIPMENT WITH ANY TAYLOR-WHARTON-APPROVED ELECTRONIC CONTROLLER, YOU MUST READ THE

SEPARATE OPERATING AND SAFETY MANUAL PROVIDED WITH THAT CONTROLLER.

*Units featured may not show final controller provided.

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LABS

TABLE OF CONTENTS

SAFETY PRECAUTIONS FOR LIQUID NITROGEN ........................................... 3

Extreme Cold - Cover Eyes and Exposed Skin ........................................................ 3

Keep Equipment Area Well Ventilated ...................................................................3

Liquid Nitrogen System .......................................................................................... 3

Dispose of Waste Liquid Nitrogen Safely ...............................................................3

GENERAL INFORMATION ............................................................................ 4

LABS Cryostorage Speciﬁcations ............................................................................ 5

INSTALLATION ............................................................................................. 6

Unpacking and Inspection ......................................................................................6

Freight Damage Procedures ..................................................................................6

Repackaging for Shipment ..................................................................................... 6

Liquid Nitrogen Supply Connection .......................................................................7

Electrical ................................................................................................................7

Power Supply Connection .....................................................................................7

OPERATION ................................................................................................. 7

Initial Fill ................................................................................................................ 8

Operating Parameters ...........................................................................................8

Liquid Phase Storage ..............................................................................................8

Thermocouple Positioning ...................................................................................... 8

Adding an Inventory Control System .....................................................................8

Inventory Control System Layouts (LABS Cryostorage System) ...............................9

MAINTENANCE ......................................................................................... 13

Strainer Cleaning Instructions .............................................................................13

Defrosting Your LABS Cryostorage System ........................................................... 13

Cleaning Your Taylor-Wharton Cryostorage System ............................................13

Normal Evaporation Rate (NER) Test ....................................................................14

TROUBLESHOOTING ................................................................................. 14

Symptoms .............................................................................................................14

REPLACEMENT PARTS ................................................................................ 14

FREEZER SERVICE AND MAINTENANCE HISTORY LOG .............................. 15

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LABS

Figure 1.0 A master computer program works with the KRYOS software

system to control and monitor this bank of LABS cryostorage units.

Figure 2.0 & 2.1 Retractable steps make retrieval of stored samples

easy and efﬁcient.

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LABS

SAFETY PRECAUTIONS FOR LIQUID NITROGEN

Nitrogen is an inert, colorless, odorless, and tasteless gas making up four-ﬁfths of the air you breathe – and can be hazardous. Air is roughly one-ﬁfth oxygen. Liquid nitrogen is at a temperature of -196°C (-320°F) under normal atmospheric pressure.

Extreme Cold - Cover Eyes and Exposed Skin

Accidental contact of liquid nitrogen or cold issuing gas with the skin or eyes may cause a freezing injury similar to frostbite. Handle the liquid so that it won’t splash or spill. Protect your eyes and cover the skin where the possibility of contact with the liquid, cold pipes and cold equipment, or cold gas exists. Safety goggles or a face shield should be worn when operating this equipment. Insulated gloves that can be easily removed and long sleeves are recommended for arm protection. Trousers without cuffs should be worn outside boots or over the shoes to shed spilled liquid.

Keep Equipment Area Well Ventilated

Although nitrogen is non-toxic and non-ﬂammable, it can cause asphyxiation in a conﬁned area without adequate ventilation. Any atmosphere not containing enough oxygen for breathing can cause dizziness, unconsciousness, or even death. Nitrogen is a colorless, odorless, and tasteless gas that cannot be detected by the human senses and will be inhaled normally as if it were air. Without adequate ventilation, the expanding nitrogen will displace the normal air resulting in death or asphyxiation.

Liquid Nitrogen System

The liquid nitrogen supply pressure at the inlet to the refrigerator should be in the range of 10 psig (0.7 bar/69 kPa) to 22 psig (1.4 bar/152 kPa) for optimum performance. Higher operating pressures will increase transfer losses and create excessive turbulence of the liquid in the refrigerator which can generate false signals to the liquid level controller causing the refrigerator to under-ﬁll. In “liquid phase” storage applications, excessive turbulence can cause splashing which could result in personal injury and/or damage to the refrigerator. When installing piping or ﬁll hose assemblies, make certain a suitable safety relief valve is installed in each section of plumbing between shut-off and solenoid valves. Trapped liqueﬁed gas will expand rapidly as it warms and may burst hoses or piping causing damage or personal injury. A relief valve is installed in the refrigerator plumbing to protect the line between the customer supplied shut-off valve and the refrigerator solenoid valve.

For more detailed information concerning safety precautions and safe practices to be observed when handling cryogenic liquids consult CGA pamphlet P-12 “Handling Cryogenic Liquids” available from the Compressed Gas Association, 1235 Jefferson Davis Highway, Arlington, VA 22202.

WARNING: The following safety precautions are for your protection. Before installing, operating, or maintaining this unit read and follow all safety precautions in this section and in reference publications. Failure to observe all safety precautions can result in property damage, personal injury, or possibly death.

Caution: When installing ﬁeld fabricated piping, make certain a suitable safety valve is installed in each section of piping between shut-off and solenoid valves.

WARNING: Inlet pressure should not exceed 22 psig (1.5 bar/152 kPa). Higher pressures could result in damage to equipment.

Dispose of Waste Liquid Nitrogen Safely

Dispose of waste liquid nitrogen out-of-doors where its cold temperature cannot damage ﬂoors or driveways and where it will evaporate rapidly. An outdoor pit ﬁlled with clean sand or gravel will evaporate liquid nitrogen safely and quickly.

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LABS

GENERAL INFORMATION

This state-of-the-art Cryogenic System can be used in either the liquid or vapor phase. Units are supplied with Taylor-Wharton approved controllers. If other liquid level controllers are used, please contact Taylor-Wharton before putting the refrigerator into service.

Taylor-Wharton LABS Cryostorage Systems are designed for applications where extremely low temperature storage of biological products is required. They are also appropriate for industrial or other applications where liquid nitrogen temperatures and high capacity are needed.

The LABS style refrigerator covered by this publication is designed for, but not limited to, the laboratory environment. Each model is supplied with locking casters to enable limited mobility for cleaning and positioning purposes.

The standard model is also equipped with a Taylor-Wharton approved electronic liquid level controller that will help monitor and control the supply of liquid nitrogen to the unit. Make sure to read the operating and saftey instructions provided with the controller you have selected to use with your LABS unit. The addition of a liquid nitrogen supply and inventory control racks for systematic retrieval of stored product completes the total Cryostorage System.

Hinged and Lockable Lid

Ergonomic

Lid Handle

High Density

Foam Plug with

Sealed Coating

Turntable

Pivot Point

with Easy Grip

Non-Bearing

Carousel

Battery Back Up Flat Table Top

Pivoting Controller

Controller

Electronics

Solenoid

Valve

Vacuum

and Super

Insulation

Low

Temperature

Storage Area

Ergonomic

Folding and

Locking Step

Locking Wheel

Casters

Figure 3.0 LABS 80K

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LABS

LABS Cryostorage Speciﬁcations

Liquid nitrogen at atmospheric pressure weighs 1.78 lb./liter (0.8 kg/liter). All Taylor-Wharton Gas Equipment CryoScience systems are designed to support the full weight of liquid nitrogen and a complete stainless steel or aluminum inventory control system with boxes and specimens.

Dimensions 20K 38K 40K 80K 94K

External Operating Height in. 42 44 42 42 40 (Top of Step to Lid opening) mm 1067 1118 1067 1067 1016

Step Height mm 279 292 279 279/432 279/559

Height in. 53 56 53 53 63 (Floor to Opening) mm 1346 1422 1346 1346 1600

Usable Height, Internal in. 30 30 30 30 35.75 mm 762 762 762 762 908

Outside Diameter in. 34 42 45 59.5 59.5 mm 864 1067 1143 1511 1511

Internal Working Diameter in. 29.5 37.8 40.5 55 55 mm 750 959 1029 1397 1397

in. 11 11.5 11 11/17 11/22

Neck Opening in. 13 18 18 24.5 24.5 mm 330 457 457 622 622

Capacity

Total LN

Capacity L 407 626 606 1350 1630

LN2 Under Capacity L 46 72 76 150 300

Power Supply

Weight, Empty

VAC 110/220 110/220 110/220 110/220 110/220

lb. 650 930 920 1550 1700

kg 295 422 417 703 771

Maximum Gross Weight

lb. 1375 2050 2000 3956 4600

kg 624 930 907 1794 2086

Inventory Control System Speciﬁcations

No. 100/81 Cell Racks 14 26 30 58 60 No. Shelves/Rack 13 13 13 13 15

No. 25 Cell Racks

4 12 8 12 8

No. Shelves/Rack 13 13 13 13 15

Vial Capacity, 2 ml

Bag 25 ml (7 Level Rack)

Bag 50 ml (7 Level Rack)

Bag 250 ml (5 Level Rack)

Bag 500 ml (5 Level Rack)

1624 2688 3108 5999 6856

924 1582 1876 3381 3864

500 900 990 1920 2304

19500 37700 41600 79300 93000

420 690 830 1610 1932

Footnotes:

Model LABS 94K has an independent two stage stainless step. Also available as an option on the LABS 80K.

Controller reduces power to 24VAC or less. Power outlet conﬁguration should be indicated at time of order.

Includes the empty weight and total LN2 capacity weight.

2 ml vial size; 12.5 mm O.D. internal thread. 6-2 half-rack capacity of 94,200.

Model LABS 94K, 8 Level Rack

Model LABS 94K, 6 Level Rack

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LABS

INSTALLATION

Unpacking and Inspection

Inspect shipping containers for external damage. All claims for damage (apparent or concealed) or partial loss of shipment must be made in writing within ﬁve (5) days from receipt of goods. If damage or loss is apparent, please notify the appropriate parties as indicated below:

Domestic LTL Shipments – The customer should notify and ﬁle the appropriate damage claims with the carrier. All products are shipped FOB Origin.

Domestic UPS Shipments – Any damage should be noted and reported to shipper upon delivery, and Taylor-Wharton must also be notiﬁed. Conﬁrm with Taylor-Wharton Customer Service the ﬁling procedures for any UPS damage claims.

Figure 4.0 Crated LABS 38K

International Shipments – Any damage

and/or claims are to be ﬁled with the carrier. Insurance agent(s) and customs’ brokers should also be notiﬁed.

In all cases, Taylor-Wharton should be notiﬁed so we can assist if needed in ﬁling damage claims.

Open the shipping containers; a packing list is included with the system to simplify checking that all components, cables, accessories, and manuals were received. Please use the packing list to check off each item as the system is unpacked. Inspect for damage. Be sure to inventory all components supplied before discarding any shipping materials. If there is damage to the system during transit, be sure to ﬁle proper claims promptly. Please advise Taylor-Wharton of such ﬁlings. In case of parts or accessory shortages, advise Taylor-Wharton immediately. Taylor-Wharton cannot be responsible for any missing parts unless notiﬁed within 60 days of shipment.

Freight Damage Procedures

Any freight damage claims are your responsibility. Cryostorage Systems are delivered to your carrier from Taylor-Wharton’s dock in new condition; when you receive our product you may expect it to be in that same condition. For your own protection, take time to visually inspect each

shipment in the presence of the carrier’s agent before you accept delivery. If any damage is observed, make an appropriate notation on the freight bill. Then, ask the driver to sign the notation before you receive the equipment. You should decline to accept containers that show damage which might affect serviceability.

Repackaging for Shipment

If it is necessary to return any part of the system for repair or replacement, a Material Return Authorization (MRA) number must be obtained from an authorized factory representative before returning the equipment to our service department. Contact your distributor for return authorization. When returning equipment for service, the following information must be provided before obtaining an MRA:

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LABS

A. System model and serial number, and controller model and unit, if available. B. User’s name, company, address, and phone number C. Malfunction symptoms

If possible, the original packing material should be retained for reshipment. If not available, consult Taylor-Wharton for shipping and packing instructions. It is the responsibility of the customer to assure that the goods are adequately packaged for return to the factory. All refrigerators returned to Taylor-Wharton must be clean and

sterile before return. See page 13 for cleaning instructions.

Liquid Nitrogen Supply Connection

The package included with the refrigerator includes a strainer. The liquid ﬁll hose from a low pressure source of liquid nitrogen must be connected to the inlet through this ﬁtting. This liquid nitrogen source must have a shut-off valve, and may be any portable liquid cylinder or a bulk supply. The liquid nitrogen supply

pressure at the inlet to the refrigerator should be in the range of 10 psig (0.7 bar/69 kPa) to 22 psig (1.4 bar/152 kPa) for optimum performance. Higher operating pressures will increase transfer losses and create excessive turbulence of the liquid in the refrigerator which can generate false signals to the liquid level controller causing the refrigerator to under-ﬁll. In “liquid phase” storage applications, excessive turbulence can cause

splashing which could result in personal injury and/or damage to the refrigerator. If the liquid nitrogen supply pressure at the inlet to the refrigerator rises above the opening pressure of the relief valve on the refrigerator, liquid nitrogen will be discharged into the surrounding area which can cause a rapid and very dangerous depletion of oxygen in the atmosphere. Once this pressure relief device has opened and cooled to liquid nitrogen temperature, it will not reseat until it has warmed to near ambient temperature. THIS COULD PERMIT THE ENTIRE CONTENTS OF THE LIQUID NITROGEN SUPPLY SYSTEM TO BE DISCHARGED INTO THE IMMEDIATE AREA OF THE REFRIGERATOR(S) (SEE WARNING). IT IS RECOMMENDED TO HAVE THE FREEZER RELIEF VALVE CONNECTED TO VENT LINE THAT EXHAUSTS TO THE OUTSIDE ATMOSPHERE.

WARNING: In order to prevent the relief device on the nitrogen refrigerator(s) from opening when the system is in operation, the liquid nitrogen supply system must be protected by a pressure relief device that will open when the pressure at the inlet to the refrigerator(s) is approximately 22 psig (1.5 bar/152 kPa). Never install the supply system pressure relief device onto a liquid service line.

Electrical

The liquid level controllers used with these refrigerators operate from 24/16.5 VAC. However, the external transformer does have a 110/220 VAC primary. Disconnect the electrical power cord from the outlet before attempting any service.

Power Supply Connection

Connect the power supply to the LABS Cryostorage System and then plug the power supply into a surge protected 110/220 VAC outlet.

OPERATION

These instructions are for operators experienced with cryogenic equipment. Before operating the system, become familiar with the safety precautions in this LABS manual and in the operating manual for the controller model you are using with your LABS unit. Make certain all applicable provisions set forth in the Installation Section have been followed before placing a system in operation. Study this manual thoroughly. Know the location and function of all system components.

WARNING: Electrical shock can kill. Do not attempt any service on these units without ﬁrst disconnecting the electrical power cord.

WARNING: Maintain adequate ventilation to prevent asphyxiation hazard (see Safety Precautions).

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LABS

WARNING: If the ﬁll fails to stop for any reason, quickly close the liquid supply valve to prevent overﬁlling until the cause of the problem can be determined.

WARNING: When working with Liquid Nitrogen, make sure to observe all safety guidelines and wear the appropriate safety gear to avoid personal injury, asphyxiation and possibly death.

Initial Fill

The LABS Cryostorage System comes preset for vapor phase storage from the factory. The liquid nitrogen supply pressure at the inlet to the refrigerator should be in the range of 10 psig (0.7 bar/69 kPa) to 22 psig (1.4 bar/152 kPa) for optimum performance. Higher operating pressures will increase transfer losses and create excessive turbulence of the liquid in the refrigerator which can generate false signals to the liquid level controller causing the refrigerator to under ﬁll. In “liquid phase” storage applications, excessive turbulence can cause splashing which could result in personal injury.

Operating Parameters

When materials are immersed in liquid nitrogen, they will assume the temperature of the liquid (-196ºC/-320ºF). When material is stored in the vapor phase over the liquid, the liquid nitrogen is still a very cold refrigerant, but the refrigerator’s interior temperature increases somewhat as product is stored higher over the liquid. This temperature differential is not signiﬁcant in many biological storage applications, and is affected by the amount of product stored in the refrigerator, the type and size of inventory control system, and the liquid level in the unit.

Liquid Phase Storage

Liquid phase storage is normally utilized when liquid nitrogen temperatures are required to maintain stored product viability and the storage medium is adequate for storage in liquid nitrogen. To reconﬁgure the system’s setting for liquid phase, review the operating instructions for the speciﬁc controller you have selected to use with your LABS unit.

Thermocouple Positioning

The thermocouple is a separate sensor used to monitor and control the temperature within the refrigerator. The factory-supplied thermocouple in most of the LABS Cryostorage Systems is positioned to monitor the temperature 1.75 in. (44.45 mm) below the top of the carousel.

Adding an Inventory Control System

The purpose of the inventory control system is to bring order to the storage of many small samples, and to allow direct retrieval of the particular sample you need at any time. It is important to be aware that when you lift an ICS rack from the refrigerator it is being moved to a warmer environment. Learn to locate your sample quickly to avoid unnecessary warming of your stored product. Keep ICS inserts (drawers or boxes) and dividers in good repair. Replacement inserts and dividers are available from your Taylor-Wharton distributor to keep your system as efﬁcient as possible.

Always wear gloves when handling ICS racks or stored product, as they are very cold. Read all of the precautions in the Safety section of these instructions and in the Taylor-Wharton publication TW-10 “Handle With Care” for more detail on handling product stored in liquid nitrogen.

When removing ICS racks to retrieve product, protect the labels, plastic, and electronic areas of the refrigerator from liquid nitrogen that may spill from the rack inserts. These parts of the refrigerator are subject to damage from the extreme low temperature of the refrigerant.

Do not let ice or debris collect in the bottom of the freezer. Schedule periodic clean out if racks no longer stand upright.

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LABS

Inventory Control System Layouts (LABS Cryostorage System)

• 100 Vial Polycarbonate

Typical Rack with

Box Design

LABS 20K LABS 38K LABS 40K

• 100 Vial Aluminum Boxes

• 81 Vial Polycarbonate,

• Cardboard, or

• Stainless Steel Boxes

Figure 5.0

Vial Storage in

Boxes on Racks

LABS 80K

LABS 94K

NOTE: Racks are not square. Orientation is important.

2 ml vial storage – ask about other Box types

Part

Number

LABS 20K - 14 - 4 182 52 19500

LABS 38K - 26 - 12 338 156 37700

LABS 40K - 30 - 8 390 104 41600

LABS 80K - 58 - 12 754 156 79300

LABS 94K 60 - 8 - 900 120 93000

Racks

15 x 2 x 100

9900-0005

Racks

13 x 2 x 100

R23K-8C35

Racks 15 x 2 x 25 9900-0006

Racks 13 x 2 x 25 R10K-9C44

Boxes-100

N-374187-92

(Case of 24

Polycarbonate)

Boxes-25

N-374180

(Case of 36

Polycarbonate)

Vial

Capacity

Max.

Allowable

Figure 5.0 Vial Storage in Boxes on Racks

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LABS

Figure 6.0 Rack Arrangements for 50 ml Cassettes

Typical

Cassette

Design

LABS 20K LABS 38K LABS 40K

LABS 80K LABS 94K

Part Number Capacity Frames Cassettes

LABS 20K 924 132 924

LABS 38K 1582 226 1582

LABS 40K 1876 268 1876

LABS 80K 3381 483 3381

LABS 94K* 3864 483 3864

Figure 6.0 Rack Arrangements for 50 ml Cassettes

*LABS 94K use an 8-level rack. All other units utilize a 7-level rack conﬁguration.

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LABS

Typical

Cassette

Design

LABS 20K LABS 38K LABS 40K

Figure 7.0

Rack Arrangements

for 250 ml Cassettes

LABS 80K LABS 94K

Part Number Capacity Frames Cassettes

LABS 20K 500 100 500

LABS 38K 900 180 900

LABS 40K 990 198 990

LABS 80K 1920 384 1920

LABS 94K* 2304 384 2304

Figure 7.0 Rack Arrangements for 250 ml Cassettes

*LABS 94K use a 6-level rack. All other units utilize a 5-level rack conﬁguration.

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LABS

Figure 8.0 Rack Arrangement for 500 ml Cassettes

Typical

Cassette

Design

LABS 20K LABS 38K LABS 40K

LABS 80K LABS 94K

Part Number Capacity Frames Cassettes

LABS 20K 420 84 420

LABS 38K 690 138 690

LABS 40K 830 166 830

LABS 80K 1610 322 1610

LABS 94K* 1932 322 1932

Figure 8.0 Rack Arrangement for 500 ml Cassettes

*LABS 94K use a 6-level rack. All other units utilize a 5-level rack conﬁguration.

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MAINTENANCE

Strainer Cleaning Instructions

The container will not ﬁll properly if the strainer is clogged with ice or dirt. To clean the strainer, ﬁrst close the supply valve to the refrigerator. Vent the ﬁll line of all pressure. Remove and warm the strainer to ambient temperature. Purge the strainer from both directions with dry nitrogen gas or dry oil-free air. Rinse the strainer with alcohol and purge it again with dry nitrogen gas or dry oil-free air to clear contaminants. If the cleaning process doesn’t clear the blockage, replace with a new ﬁlter (P/N 7631-1080).

Defrosting Your LABS Cryostorage System

All liquid nitrogen storage systems are subject to ice and frost buildup over time. Regular preventive maintenance programs should be instituted to remove ice and frost from the sensor and ﬁll tubes and from the refrigerator lid. Ice and frost buildup in the sensor tube may result in false readings being relayed to the controller from the sensors. Ice can form a thermal barrier around a level sensor, rendering it insensitive to the temperature differences between vapor and liquid. If the ﬁll line becomes blocked, it must be warmed until the ice blockage is cleared.

Ice and frost buildup in the ﬁll tube may block the ﬂow of liquid nitrogen into the refrigerator during ﬁll. This blockage can result in the liquid level dropping to dangerously low levels, and may result in the Low Alarm sensor being activated. In addition, a ﬁll line blockage may cause the Low LN

If it is determined that defrosting is necessary the following procedure must be followed. Cryogenic freezers of these types must be carefully warmed to ambient temperatures to avoid the ingress of water. As long as liquid nitrogen is present in the unit, the evaporation of the liquid nitrogen provides slightly positive gas pressure in the unit, preventing the entrance of moisture laden air. When all of the liquid nitrogen has evaporated but the unit temperature is still below the dew point of the surrounding atmosphere, condensation will start to collect in the unit. When frost and condensation collect in the unit, staining of the carousel or damage to the level sensors will occur. If one of these units is to be taken out of service for any reason the following steps must be performed to avoid damaging the unit.

Supply Alarm to be activated.

LABS

1. Disconnect power and liquid nitrogen source.

2. Remove all stored product and inventory control system components.

3. Remove Sensors.

4. A source of nitrogen gas must be found and used to purge the unit as the temperature rises to ambient. The purge gas can be installed on the sensor tube or ﬁll tube or into the liquid via the trap door in the bottom of the carousel after the appropriate components have been removed. The purge gas may also simply be delivered to the unit through a bent metal tube under the lid. A ﬂow rate of 5 to 10 SCFM is sufﬁcient to prevent water from collecting inside the unit. This ﬂow of purge gas will also speed the warming of the unit to ambient temperature.

Cleaning Your Taylor-Wharton Cryostorage System

The cryogenic vessel of all LABS Cryostorage Systems may need to be cleaned, and sterilized if the type of stored product is changed or the unit is taken out of service.

The vessel must be defrosted, cleaned, and sterilized regardless of the type of stored product, prior to return to Taylor-Wharton for repair or maintenance.

Prior to cleaning, the unit must be warmed and defrosted as described in the previous section.

WARNING: Never use chlorinebased disinfectants to clean a LABS Cryostorage System.

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LABS

To clean and sterilize your defrosted LABS Cryostorage System, spray the entire inner vessel surface with ample amounts of an approved & compatible disinfectant. Allow surface contact to be maintained for a minimum of ﬁve minutes. Rinse the inner vessel with water, remove all water and debris through the access opening located in the carousels bottom tray, and vacuum or towel dry the surface. Spray the inner vessel surface with a 70% alcohol to water solution and maintain surface contact for ﬁfteen minutes. Rinse the inner vessel surface with water and vacuum or towel dry excess water. Final dry the inner container, sensor line, and ﬁll line using a nitrogen gas purge.

Normal Evaporation Rate (NER) Test

WARNING: Never use hollow rods or tubes as dipsticks. When a warm tube is inserted into liquid nitrogen, liquid will spout from the top of the tube and may cause personal injury.

WARNING: The source power supply at 110/220 can cause a lethal electrical shock. Unplug the power cord before beginning any repairs.

Nitrogen consumption is an accumulation of all system components and user introduced evaporation. The storage chamber is a double walled, vacuum insulated vessel and contributes to the daily consumption of liquid nitrogen. The liquid nitrogen supply vessel and transfer hose also contribute greatly to the daily consumption rate. Choosing to control the vapor temperature, combined with the liquid level and temperature speciﬁed, will affect the overall nitrogen consumption. In addition to these variables, opening the lid to retrieve product, and adding new product into the storage chamber will pay a role in the accumulative liquid nitrogen consumption.

If the nitrogen consumption of your Cryostorage System seems excessive, it may be appropriate to perform an estimated Normal Evaporation Rate (NER) test on the Cryostorage chamber. To perform an NER test:

1. Fill the Cryostorage unit to the “High Level” sensor.

2. Measure the liquid nitrogen level with a plastic or wooden measuring rod.

3. Close and lock the lid of the LABS Cryostorage System for forty-eight (48 hours).

4. Open the LABS Cryostorage System and measure the liquid nitrogen level. Typically, liquid nitrogen levels will drop approximately 3/8 in. (9.53 mm) per day. If your measurement indicates a drop in excess of 3/4 in. (19 mm) per day, please contact your Taylor-Wharton distributor or Taylor-Wharton for further information. Please have your serial number, this manual and service history available.

TROUBLESHOOTING

Symptoms

The key to troubleshooting your LABS Cryostorage System is to determine which component in the system is the source of the problem. Utilizing this manual along with your controller’s manual, determine if the problem is occurring in any of the following subsystems: Supply Vessel, Transfer Line, Power Source, Temperature, Level Sensing, Security, Lid Switch, Solenoid Valve, Control Display, Alarm System, Communications. After determining which sub-system is having the problem, isolate the problem further by performing sub-system tests. Once the problem is isolated and deﬁned, it will be easier to solve.

REPLACEMENT PARTS

A complete list of Replacement Parts and Accessories for the LABS Series is available from the following Taylor-Wharton Customer Care Centers:

Americas +251-443-8680 In U.S. and Canada: 800-898-2657 Europe +421 (0)55-727-7157 Asia/Australia +61 (0)2 6040-2533

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LABS

FREEZER SERVICE AND MAINTENANCE HISTORY LOG

Fill in top section at installation. Copy this form each time service is required. Fill bottom section with service notes to keep a complete log of each freezer service and maintenance history.

End User Company Name _________________________________ LOG NO ______________________________

Service Contract/NameCompany ___________________________________________________________________

Service Contract Phone Number/Fax ________________________________________________________________

LABS-series Model/Serial Number __________________________________________________________________

Controller Serial Number__________________________________________________________________________

Control Version Number __________________________________________________________________________

In-service Date ___________________________________________________________________________________

Describe Conditions – Actual Describe Conditions – Controller Reading

Liquid Level – via Dipstick ______________________ Liquid Level – Per Controller _______________________

Level Sensor Type _____FG _____ 8T _____ 4T _____ Liquid Level Setting _____ HIGH _____LOW

Lid _____ Open _____ Closed Lid _____Open _____ Closed

Filling _____ Yes _____ No Filling ______Yes______No

Temperature _________________________________ Temperature _____________________________________

Supply ______________ ltr______________ psi Supply Alarm ______On ______ Off

Note: Ice Build-up _____ a little _____a lot Remote Alarm ______ On ______ Off

Note: Gasket condition _____ Seals _____ Leaks Audible Alarm ______ On ______ Off

Display Lights _____ On _____ Off Temp. control setpoint @ __________ degrees

Taylor-Wharton Technical Services - Phone: In USA & Canada 800-898-2657

Fax: +251-443-2209 USA • +421 (0)55-727-7157 Kosice • +49 (0)4841-985130 Husum

Service History Log (note date and log number on each service entry)

Date: ___________________________________________________________________________________________

NOTE: All Taylor-Wharton Cryostorage Systems must be cleaned and sterilized prior to return to Taylor-Wharton for repair or maintenance and must be accompanied by a written statement to this effect. Any LABS-Service Cryostorage System received without this statement will be returned to the sender, freight collect. Contact Customer Service by telephone: 800-898-2657 In USA & Canada or

+421 (0)55-727-7124 Kosice or +49 (0)4841-985139 Husum. Or email: salesandservice@taylorwharton.com.

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4075 Hamilton Boulevard, Theodore AL 36582

TWSalesandService@taylorwharton.com

www.taylorwharton.com

CryoScience Information Centers: Europe Americas Asia/Australia

 +421(0)55-727-7157 +251-443-8680•InU.S.andCanada:800-898-2657 +61(0)26040-2533

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Taylor-Wharton LABS Series User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual