Taylor-Wharton LABS Series User Manual
LABS
(Laboratory Archival Biological Storage)
OPERATION & MAINTENANCE INSTRUCTIONS
CRYOSTORAGE SYSTEM
CAUTION - SAFETY FIRST!
• REVIEW AND UNDERSTAND ALL SAFETY PROCEDURES IN THE “HANDLE WITH
CARE” BOOKLET FORM # TW-10 P/N 7950-8052 BEFORE ATTEMPTING TO
INSTALL, OPERATE OR PERFORM MAINTENANCE ON THIS CRYOSTORAGE SYSTEM.
• DO NOT ATTEMPT TO USE OR MAINTAIN THIS UNIT UNTIL YOU READ AND
UNDERSTAND THESE INSTRUCTIONS.
• DO NOT PERMIT UNTRAINED PERSONS TO USE OR MAINTAIN THIS UNIT.
• IF YOU DO NOT FULLY UNDERSTAND THESE INSTRUCTIONS, CONTACT YOUR
SUPPLIER FOR FURTHER INFORMATION.
• BEFORE ATTEMPTING TO OPERATE THIS EQUIPMENT WITH ANY
TAYLOR-WHARTON-APPROVED ELECTRONIC CONTROLLER, YOU MUST READ THE
SEPARATE OPERATING AND SAFETY MANUAL PROVIDED WITH THAT CONTROLLER.
*Units featured may not show final controller provided.
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LABS
TABLE OF CONTENTS
SAFETY PRECAUTIONS FOR LIQUID NITROGEN ........................................... 3
Extreme Cold - Cover Eyes and Exposed Skin ........................................................ 3
Keep Equipment Area Well Ventilated ...................................................................3
Liquid Nitrogen System .......................................................................................... 3
Dispose of Waste Liquid Nitrogen Safely ...............................................................3
GENERAL INFORMATION ............................................................................ 4
LABS Cryostorage Specifications ............................................................................ 5
INSTALLATION ............................................................................................. 6
Unpacking and Inspection ......................................................................................6
Freight Damage Procedures ..................................................................................6
Repackaging for Shipment ..................................................................................... 6
Liquid Nitrogen Supply Connection .......................................................................7
Electrical ................................................................................................................7
Power Supply Connection .....................................................................................7
OPERATION ................................................................................................. 7
Initial Fill ................................................................................................................ 8
Operating Parameters ...........................................................................................8
Liquid Phase Storage ..............................................................................................8
Thermocouple Positioning ...................................................................................... 8
Adding an Inventory Control System .....................................................................8
Inventory Control System Layouts (LABS Cryostorage System) ...............................9
MAINTENANCE ......................................................................................... 13
Strainer Cleaning Instructions .............................................................................13
Defrosting Your LABS Cryostorage System ........................................................... 13
Cleaning Your Taylor-Wharton Cryostorage System ............................................13
Normal Evaporation Rate (NER) Test ....................................................................14
TROUBLESHOOTING ................................................................................. 14
Symptoms .............................................................................................................14
REPLACEMENT PARTS ................................................................................ 14
FREEZER SERVICE AND MAINTENANCE HISTORY LOG .............................. 15
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LABS
Figure 1.0 A master computer program works with the KRYOS software
system to control and monitor this bank of LABS cryostorage units.
Figure 2.0 & 2.1 Retractable steps make retrieval of stored samples
easy and efficient.
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LABS
SAFETY PRECAUTIONS FOR LIQUID NITROGEN
Nitrogen is an inert, colorless, odorless, and tasteless gas making up four-fifths of
the air you breathe – and can be hazardous. Air is roughly one-fifth oxygen. Liquid
nitrogen is at a temperature of -196°C (-320°F) under normal atmospheric pressure.
Extreme Cold - Cover Eyes and Exposed Skin
Accidental contact of liquid nitrogen or cold issuing gas with the skin or eyes may
cause a freezing injury similar to frostbite. Handle the liquid so that it won’t splash
or spill. Protect your eyes and cover the skin where the possibility of contact with the
liquid, cold pipes and cold equipment, or cold gas exists. Safety goggles or a face
shield should be worn when operating this equipment. Insulated gloves that can be
easily removed and long sleeves are recommended for arm protection. Trousers
without cuffs should be worn outside boots or over the shoes to shed spilled liquid.
Keep Equipment Area Well Ventilated
Although nitrogen is non-toxic and non-flammable, it can cause asphyxiation in a
confined area without adequate ventilation. Any atmosphere not containing enough
oxygen for breathing can cause dizziness, unconsciousness, or even death. Nitrogen is
a colorless, odorless, and tasteless gas that cannot be detected by the human senses
and will be inhaled normally as if it were air. Without adequate ventilation, the
expanding nitrogen will displace the normal air resulting in death or asphyxiation.
Liquid Nitrogen System
The liquid nitrogen supply pressure at the inlet to the refrigerator should be in
the range of 10 psig (0.7 bar/69 kPa) to 22 psig (1.4 bar/152 kPa) for optimum
performance. Higher operating pressures will increase transfer losses and create
excessive turbulence of the liquid in the refrigerator which can generate false signals
to the liquid level controller causing the refrigerator to under-fill. In “liquid phase”
storage applications, excessive turbulence can cause splashing which could result in
personal injury and/or damage to the refrigerator. When installing piping or fill hose
assemblies, make certain a suitable safety relief valve is installed in each section of
plumbing between shut-off and solenoid valves. Trapped liquefied gas will expand
rapidly as it warms and may burst hoses or piping causing damage or personal
injury. A relief valve is installed in the refrigerator plumbing to protect the line
between the customer supplied shut-off valve and the refrigerator solenoid valve.
For more detailed information concerning safety precautions and safe practices
to be observed when handling cryogenic liquids consult CGA pamphlet P-12
“Handling Cryogenic Liquids” available from the Compressed Gas Association,
1235 Jefferson Davis Highway, Arlington, VA 22202.
WARNING:
The following safety
precautions are for
your protection.
Before installing,
operating, or
maintaining this unit
read and follow all
safety precautions
in this section
and in reference
publications. Failure
to observe all safety
precautions can
result in property
damage, personal
injury, or possibly
death.
Caution:
When installing field
fabricated piping,
make certain a
suitable safety valve
is installed in each
section of piping
between shut-off and
solenoid valves.
WARNING:
Inlet pressure should
not exceed 22 psig
(1.5 bar/152 kPa).
Higher pressures
could result
in damage to
equipment.
Dispose of Waste Liquid Nitrogen Safely
Dispose of waste liquid nitrogen out-of-doors where its cold temperature cannot
damage floors or driveways and where it will evaporate rapidly. An outdoor pit
filled with clean sand or gravel will evaporate liquid nitrogen safely and quickly.
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LABS
GENERAL INFORMATION
This state-of-the-art Cryogenic System can be used in either the liquid or vapor
phase. Units are supplied with Taylor-Wharton approved controllers. If other
liquid level controllers are used, please contact Taylor-Wharton before putting the
refrigerator into service.
Taylor-Wharton LABS Cryostorage Systems are designed for applications where
extremely low temperature storage of biological products is required. They are also
appropriate for industrial or other applications where liquid nitrogen temperatures
and high capacity are needed.
The LABS style refrigerator covered by this publication is designed for, but not limited
to, the laboratory environment. Each model is supplied with locking casters to
enable limited mobility for cleaning and positioning purposes.
The standard model is also equipped with a Taylor-Wharton approved electronic
liquid level controller that will help monitor and control the supply of liquid nitrogen
to the unit. Make sure to read the operating and saftey instructions provided with
the controller you have selected to use with your LABS unit. The addition of a liquid
nitrogen supply and inventory control racks for systematic retrieval of stored product
completes the total Cryostorage System.
Hinged and Lockable Lid
Ergonomic
Lid Handle
High Density
Foam Plug with
Sealed Coating
Turntable
Pivot Point
with Easy Grip
Non-Bearing
Carousel
Battery Back Up Flat Table Top
Pivoting Controller
Controller
Electronics
Solenoid
Valve
Vacuum
and Super
Insulation
Low
Temperature
Storage Area
Ergonomic
Folding and
Locking Step
Locking Wheel
Casters
Figure 3.0 LABS 80K
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