Taylor-Wharton CX Series User Manual

Operating Instructions

SAFETY

Before using any cryogenic refrigerator, read
the Handle with Care booklet provided with the
unit. It details safety precautions that must be
understood before using the equipment. If a
replacement booklet is needed, order publica­tion TW-10 Handle with Care from your sup­plier.

Following are a few of the safety precautions
described in the Handle with Care booklet.
Please be sure to read the entire booklet.

Store and use these containers only in well
ventilated areas. In a confined area, nitrogen

gas from these units may cause suffocation by
displacing air needed for breathing. Install a
suitable oxygen monitor .

Do not touch liquid or cold metal surfaces
with your bare skin. The liquid nitrogen
refrigerant in these containers is extremely
cold: -196°C (-320°F). Exposure of skin or eyes

to liquid, cold gas or frosted parts could result in
a severe frostbite-like injury . Because of the
extremely low temperature, a face shield and
gloves must be worn when transferring liquid
nitrogen and material into or out of these
containers.

Use only the necktube core supplied with
this unit or a listed replacement part. A tight

fitting plug of stopper will cause a pressure
increase in the container that may damage the
container and/or cause personal injury .

Dispose of liquid nitrogen only in areas
specifically designed for that purpose.
Disposal of liquid nitrogen should be done
outdoors in a safe place. Pour the liquid
slowly on gravel or bare earth where it can
evaporate without causing damage.

OPERATION

Important note: Before filling the CX for the
first time, record the tare weight and serial
number of each container . Recording the
“as received” tare weight on the container
itself is recommended.

Filling: Adding liquid nitrogen to a warm

container may cause splashing and will gener­ate a significant volume of nitrogen gas as cold
liquid contacts warm refrigerator surfaces. Add
liquid slowly to minimize these effects. Be sure
there is adequate ventilation. Keep your head
clear of the heavy volume of vapor that may be
produced. It is extremely cold and could cause
personal injury.

moisture will displace nitrogen in the absor­bent materials on the subsequent filling and
effect the overall holding time.

CAUTION: When filling the Cryogenic Refrigerator,
avoid liquid nitrogen coming in contact with the
vacuum plug. In order to accomplish this, the Cryo­Exchange must be removed from its shipping carton
during the “fill” operation.

Remove the unit from its shipping enclo­sure. Set the unit on a scale and fill the

refrigerator with liquid to the bottom of the
necktube. Then allow the unit to stand undis­turbed while the refrigerant is being absorbed.
About every five minutes, add liquid to main­tain the refrigerant level as the liquid is
absorbed by the filler. This procedure can t ake
ten to fifteen cycles. When the liquid levels
remains at the bottom of the necktube, the
shipper is full. At this point, the quantity of
liquid standing in the central cavity of the
refrigerator must be poured out to prevent
spillage during shipment. To assure a com­plete fill, the dry CX100 weight should in­crease by approximately 7.9 lb. (3.7 kg) and
the dry CX500 weight should increase by
approximately 1 1.5 lb. (5.2 kg).

The material to be stored in the Cryo-Ex­change Refrigerator, as well as the canister or
rack, should be pre-cooled before being
placed in the shipper. When the refrigerator is
fully charged, place the pre-cooled material to
be stored in the pre-cooled canister and lower
it into the necktube. Install the necktube core
and cap to hold the canister in place.

CAUTION: When the Cryo-Exchange is in use, it must
be kept upright. When properly filled, there is no
concern for liquid refrigerant spills. However, holding
time will be drastically reduced when the refrigerant
is on its side.

Securing contents: The contents of all
models may be secured with a seal or lock
through tabs on the edge of the lid.

ROUTINE CARE AND MAINTENANCE

Do not attempt to fasten any device to the
container . Welding, brazing, or piercing of the
container in any manner will cause permanent
damage.

TW-347

Cryo-Exchange

Vapor Shippers

WARNING
DO NOT OVERFILL. Over-filling may result in personal
injury due to liquid spillage.

With regular usage, moisture can accumulate in
the refrigerator as a result of lowering a frosted
canister back into the shipping cavity . This

CX SERIES

Refrigeration depends on the presence of cryogenic
nitrogen refrigerant in the refrigerator. Be sure to main­tain a full charge of refrigerant to prevent loss of stored
materials. Please refer to the “Operations” section of
this document for instructions on the filling procedure. If
you are not able to fully charge the Cryo-Exchange by
weight, it is possible that more moisture has accumu­lated in the filler material over time with normal usage.
This accumulation will result in the displacement of
refrigerant. Allow the refrigerant to warm to room
temperature. The warming procedure may take as long
as four weeks. Then direct dry heat not to exceed 150°F
(65°C) from hair dryer to other source, into the refrigera­tor. The unit is accept ably dry when the tare weight is
within 1 lb. (0.45 kg) of the “as received from the
manufacturer” tare weight.

If high evaporation rates are apparent under normal
operating conditions, the dewar may be losing its
vacuum. Sweating and the formation of frost on the
outer casing are indications the refrigerator may be
losing its vacuum. All necessary step s should be taken
to protect the refrigerator’s contents. If these conditions
persist, contact your supplier or Taylor-Wharton’s
Technical Response Team (1-800-TW-TANKS or email
us at

[e-mail]) for information on

how to conduct a normal evaporation rate (NER) test in
the field.

TRANSPORT ATION

Although these refrigerators are rugged, they can be
damaged or abused or otherwise mishandled. A

specially designed hard-shell shipping container (refer to
the parts section for ordering information) should be
used anytime the refrigerator is transported. This
container will help prolong the life of the unit and should
be replaced if damaged during use. When moving or

transporting a refrigerator, t ake every precaution to prevent
sliding, tipping, bumping or dropping the unit. Keep the unit
upright. Although there is no safety hazard if the properly
filled refrigerator is on its side, the refrigerant consumption is
much greater with the unit in this position and holding time
will be significantly reduced.

RETURNS

Manufacturing defects are covered under the containers
limited warranty . Evidence of mishandling, such as dents on
the outer vessel, or misalignment of the inner vessel, are not
manufacturing defects. If you would like to return goods to
Taylor-Wharton for any reason, you must first obtain a
Material Return Authorization (MRA) number for tracking
purposes.

ACCESSORIES

The following accessories are available for CX Series
Refrigerators:

• Roller Base - will ball-bearing swivel casters to provide

convenience and portability within a working area where
frequent container movement is necessary or desirable.

P/N R018-8C00 ...............................CX500

• Inventory Control Boxes - made of cardboard or

plastic to hold 2ml vials. Can be used to store samples.

(Plastic) 100 cell box

P/N N-374187-92.............................CX500

• Rugged Shipping Enclosures - for easy transport.

P/N 3701-9277(padded carton)..........CX100

P/N CX10-8C00 (Hard shell)............CX100

P/N CP19-8C00 (Hard shell)............CX500

REPLACEMENT P ARTS

Description CX100 CX500

Handle R005-5C11 N/A
Handle Pin 8631-5000 N/A
Canister R005-9C24 Note*
Necktu be Core R005-9C21 CP70-9C 18
Hard Shell Shipping Enclosure CX10-8C 0 0 CP19-8C 00

Note*: Five shelf rack (for TW plastic box).........RS30-9C40

Blood Bag Rack……………………………….......CP70-9C44

ORDERING INFORMA TION

Order all replacement parts and accessories from your distributor . Please include the p art and model number of your
refrigerator, the p art number, quantity, and description of each part requested. for more information cont act Taylor-Wharton
at the address listed.

Taylor-Wharton

4075 Hamilton Blvd.
Theodore, AL 36582

Phone: [phone]

(800) TW TANKS (898-2657)

Email: [e-mail]

www.taylorwharton.com

Fax: [phone]

7950-8347 TW-347