PerkinElmer Lambda XLS User Manual

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UV-VIS SPECTROSCOPY

LAMBDA XLS/XLS+

User’s Guide

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Release History

Part Number Release Publication Date

L6050017 D January 2014

Any comments about the documentation for this product should be addressed to: User Assistance

PerkinElmer Ltd Chalfont Road Seer Green Beaconsfield BUCKS HP9 2FX United Kingdom

Or emailed to: info@perkinelmer.com

Notices

The information contained in this document is subject to change without noti ce .

Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no warranty of any kind with regard to this document, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose.

PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing, performance or use of this material.

This document contains proprietary information that is protected by copyright. All rights are reserved. No part of this publication may be reproduced in any form whatsoever or translated into any language without the prior, written permission of PerkinElmer, Inc.

Trademarks

Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are protected by law.

PerkinElmer is a registered trademark of PerkinElmer, Inc.

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Table of Contents

Introduction ...................................................................................... 5

About this Manual .................................................................................... 6

Conventions Used in this Manual ............................................................... 7

Notes, cautions and warnings ............................................................. 7

Safety Information ........................................................................... 9

General Safety ....................................................................................... 10

Decontamination Certificates ............................................................ 11

Chemicals ....................................................................................... 11

Toxic Fumes .................................................................................... 11

Waste Disposal ................................................................................ 12

Personal Protective Equipment ......................................................... 12

Intended User ................................................................................. 12

Unpacking and Installation ...................................................................... 13

Installing your Instrument ............................................................. 15

Unpacking ............................................................................................. 16

Connecting to the Line Power Supply....................................................... 17

Using your Instrument .................................................................... 19

Overview of the Instrument .................................................................... 20

Switching on the Instrument ................................................................... 22

Using the Software ................................................................................. 23

Methods and Favorites ........................................................................... 27

Folder Options ................................................................................. 27

Method Options ............................................................................... 30

Lambda XLS/XLS+ Instrument Methods ................................................... 32

Utilities .................................................................................................. 33

Date and Time ................................................................................ 34

Regional.......................................................................................... 34

Printer ............................................................................................ 35

Preferences ..................................................................................... 36

Contrast .......................................................................................... 37

About ............................................................................................. 37

Sample Handling .................................................................................... 38

Instrument Methods ....................................................................... 39

Single Wavelength ................................................................................. 40

Multi Wavelength ................................................................................... 43

Spectrum ............................................................................................... 46

Concentration ........................................................................................ 51

Standard Curve ...................................................................................... 56

Kinetics ................................................................................................. 62

Absorbance Ratio ................................................................................... 67

Accessories...................................................................................... 71

Accessories and Consumables ................................................................. 72

Printer Installation and Configuration ...................................................... 73

Printer installation ............................................................................ 73

Printer configuration ........................................................................ 74

Loading or changing the printer paper .............................................. 75

Bluetooth Accessory Installation .............................................................. 76

Lambda Bio/XLS Print Utility.................................................................... 79

Maintenance.................................................................................... 81

General Maintenance .............................................................................. 82

Cleaning the instr ument ................................................................... 82

Storage and Shipping ............................................................................. 84

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Appendices ...................................................................................... 85

Appendix 1: Specifications ....................................................................... 86

Appendix 2: Customer Care Contacts ....................................................... 87

Index ..................................................................................................... 89

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Introduction

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6 . Lambda XLS/XLS+ User's Guide

About this Manual

This user’s guide describes the installation and operating procedures for your Lambda XLS/XLS+ UV/Vis spectrometer.

The manual contains the following sections:

• Introduction;

• Safety Information;

• Installing your Instrument;

• Using your Instrument;

• Instrument Methods ;

• Accessories;

• Maintenance;

• Appendices.

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Introduction . 7

personal injury serious damage to the instrument

Conventions Used in this Manual

Normal text is used to provide information and instructions. Bold text refers to text that is displayed on the screen. UPPERCASE text, for example ENTER or ALT, refers to keys on the PC keyboard. The

symbol “+” is used to show that you have to press two keys at the same time, for example, ALT+F.

All eight digit numbers are PerkinElmer part numbers unless stated otherwise. Unless stated otherwise, in this manual “instrument” refers to the Lambda XLS or

Lambda XLS+ instrument.

Notes, cautions and warnings

Three terms, in the following standard formats, are also used to highlight special circumstances and warnings.

NOTE: A note indicates additional, significant information that is provided with so me

procedures.

We use the term WARNING to inf orm you about situations that could

to yourself or other persons. Details about

WARNING

result in these circumstances are in a box like this one.

We use the term CAUTION to inform you about situations that co uld

CAUTION

result in

or other equipment.

Details about these circumstances are in a box like this one.

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Safety Information

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10 . Lambda XLS/XLS+ User's Guide

the sample chamber during sample measurements. Under normal use,

removed for cleaning only when

General Safety

The Lambda Bio/XLS instruments conform to the requirements of the Low Voltage Directive 2006/95/EC, EMC Directive 2004/108/EC and the IVD Directive 98/79/EC.

Standards to which conformity is declared:

• EN 61010-1 Safety requirements for electrical equipment for measurement, control and laboratory use.

• EN 61010-2-101 Particular requirements for IVD medical equipment.

• BS EN 591 Instruction for use for in vitro diagnostic instruments for professional use.

• BS EN 13612 Performance evalua tion of in vitro diagnostic medical devices.

• EN 61326-2.3 Electromagnetic compatibili ty – generic emission standard Electr ical

equipment for measurement, control and laboratory use.

• EN 61326 Electromagnetic compat ibility – generic emission standard Electrical equipment for measurement, control and laboratory use. Classified as Class B for conducted and radiated emissions.

The Lambda Bio/XLS complies with the following environmental legislation regarding the restriction of the use of certain hazardous substances:

• Europe: EU directive 2002/ 95/EC (RoHS Directive).

• China: Administration on the Control of Pollution Caused by Electronic Informatio n

Products No 39.

This appliance is marked according to the European directive 2002/96/EC on Waste Electrical and Electronic Equipment (WEEE). See the more information.

There are a number of warning label s and symbols on your instrument. These are there to inform you where potential dange r exists or particular caution is required. Before commencing installation, please take tim e to familiarize yourself with these symbols and their meaning.

This instrument is subject to the following hazards :

Lambda Bio/XLS Safety Manual

(L6050014) for

The UV source contained within the unit generates a light beam that passes through this beam is contained within the unit and no UV radiation is emitted from the system. However, care should be taken that no reflective items are placed in the beam during sample measurements so that the beam is reflected out of the system. Also, the sample holder should be the power is disconnected from the instrument. Prolonged exposure to the beam may cause permanent eye damage.

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Safety Information . 11

• High voltages exis t inside the unit. Repair and maintenance should only be carried out by individuals trained specifically to work on these instruments.

There are no bio-hazardous materials within the unit; however, this unit could be used with bio-hazardous samples. Given the general-purpose nature of the instruments it is not possible to define exact decontamination procedures for the many types of samples being tested. These should be established by the user, or their Health and Safety representative, before use of the instrument. Guidance can be found in document

from Occupationally Acquired Infections: Approved Guidelines

the US Clinical and Laboratory Standards Institute. The sample compartment may be removed and cleaned by soaking in a disinfectant appropriate for the bio-hazard for at least 30 minutes. The compartment should then be rinsed in distilled water and allowed to dry. Similarly, the outer cover can be wiped over with an appropriate disinfectant with a wipe or soft cloth. Any internal spills should be wiped up using absorbent material through the sample compartment aperture, and then similarly decontaminated using disinfectant soaked wipes.

M29-A3 Protection of Laboratory Workers

published by

Decontamination Certificates

If the instrument needs to be returned, a decontamination certificate is required. Decontamination certificates can be found at the following web address or be provide d by Customer Care.

http://www.perkinelmer.com/LambdaBioXLSSupport It is the responsibility of the user to ensure a safe working environment is provided to the

engineer or staff by appropriate risk assessments and, if appropriate, decontamination procedures before work commences.

Chemicals

Use, store, and dispose of chemicals that y ou require for your analyses in accordance wi th the manufacturer’s recommendations and local safety regulations.

Toxic Fumes

If you are working with volatile solvents or toxic substances, then you must provide an efficient laboratory ventilation system to remove vapors that may be produced when you are performing analyses.

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Waste Disposal

Waste containers may contain corrosive or organic solutions and small amounts of the substances that were analyzed. If these materials are toxic, you may have to treat the collected effluent as hazardous waste. Refer to your local safety regulations for proper disposal procedures.

Personal Protective Equipment

No Personal Protective Equipment (PPE) is required for operation of the equipment, although the samples under test may require the use of such PPE and should be cove red by a risk assessment made by the user or the appropriative Health and Safety representative.

Intended User

This instrument is intended for use by individuals trained in and familiar with the use of spectrophotometers and their associated hazards as detailed above. In the event of a malfunction or hazard occurring, the user responsible shall disconnect the unit from power, and isolate the instrument for decontamination by an appropriate means if biohazardous material is spilled on, in or around the instrument.

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Safety Information . 13

e protection

Unpacking and Installation

• This equipment is designed to be installed in accordance with the Canadian Electrical Code (CEC) Part 1, CSA C22.1 and CSA C22.2 No. 0.

• Inspect the instrument for any signs of damage caused in transit. If any damage is discovered, access the Troubleshooting Guide on the PerkinElmer website http://www.perkinelmer.com/LambdaBioXLSSupport and follow the process there. If you do not have access to the webs ite, contact Customer Care (see

Customer Care Contacts

• Ensure your proposed installation site conform s to the environmental conditions for safe operation:

Indoor use only. Temperature range 5 °C to 35 °C. Maximum relative humidity of 80% up to 31 °C, decreasing linearly to 50% at 40 °C.

NOTE: If you use the instrument in a room subjected to extremes of temperature change

during the day, it may be necessary to recalibrate (by switching off and then on again) once thermal equilibrium has been established (2–3 hours).

on page 87).

Appendix 2:

• If the instrument has just been unpacked or has been stored in a cold environment, it should be allowed to come to thermal equilibrium for 2–3 hours in the laborator y before turning the instrument on. This will prevent ca lib r ation failure as a result of internal condensation.

• The instrument must be placed on a stable, level bench or table that can take its weight (approximately 4.5 kg) with enough space around the instrument so that air can circulate freely.

• This equipment must be connected to the supply outlet with the power cord and power supply provided. It can be used on 100–240 V, 50–60 Hz supplies. The power cord or power supply outlet should be is fused at 3 A, 250 V.

• The instrument should be positione d so that the power cable can be readily disconnected from the unit in the event of a hazard or malfunction occurring.

• Site the instrument in an atmosphere free from dust and corrosive fumes.

• Please read through the instrument User’s Guide suppl ied on the Lambda Bio/XLS

Documentation CD (L6050018) prior to use.

• Switch on the instrument via the ON/OFF button on the keypad after it has been plugged in. The instrument will perform a serie s of self-diagnostic checks.

If the equipment i s used in a manner not specified herein, then t h provided by the equipment may be impaired and instrument warranty withdrawn.

WARNING

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Page 15

Installing yo ur Instrument

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Unpacking

Take great care when installing your instrument, and follow the

CAUTION

1. Check that you have the items listed in the following table.

Part Number Description

L7110189/L7110192 Lambda XLS/XLS+ (if applicable)

L7110190/L7110193 Lambda XLS/XLS+ with printer (if applicable)

L7110191/L7110194 Lambda XLS/XLS+ with SD (if applicable)

L6050014 Lambda Bio/XLS Safety Manual

procedures described in this manual.

L6050018 Lambda Bio/XLS Documentation CD

L7111025 Cuvette dust co ver

L7110231 Lambda Bio/XLS Print Utility and Cable (if applicable)

L7110270 UV macro cuvettes (8 pack)

N/A Power cable

2. Examine the components for any sig ns of damage in shipment:

• Check the enclosure of the instrument for damage, and make sure that the terminals are not damaged.

• Make sure that the compartment is free of dust or other foreign matter.

NOTE: In the event of damage, or missi ng parts, acce ss the Troubleshooting Guide on the

PerkinElmer website at http://www.perkinelmer.com/LambdaBioXLSSupport and follow the process there. If you do not have access to the website, contact Customer Care (see

Appendix 2: Customer Care Contacts

on page 87).

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Installing your Instrument . 17

Electrical Hazard

USB cable connection

Line power in

Connecting to the Line Power Supply

To prevent potential injury to yourself and damage to the instrument,

WARNING

 Connect to the supply outlet with power supply provided and the power cord that is

appropriate for your region. You must use the power cord and power supply supplied with the instrument.

NOTE: If the instrument has just been unpacked or has been stored in a cold environment,

it should be allowed to come to thermal equilibrium in the laboratory for 2–3 hour s before turning the instrument on. This will prevent calibration failure as a result of internal condensation.

make any electrical connections in the system before connecting to the line power supply.

The instrument automatically adjusts to the correct operating voltage.

Figure 1 Rear connections on the Lambda Bio/XLS instrument

The ON/OFF switch is located at the top left-hand of the keypad panel.

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Using your Instrument

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Arrow keys

Alphanumeric keys

Cancel/Back/Stop

Run/OK/Next/Scan

0A/100%T (scan reference)

Display panel

ON/OFF

View Options

Overview of the Instrument

The Lambda XLS/XLS+ instrument is a simple-to-use UV/Visible spectrometer with a CCD array detector (1024 pixels).

To access the folders and functions on your Lambda XLS/XLS+ instrument, navigate around the display using the alphanumeric keys and the navigation arrows on the keypad.

Figure 2 Keypad and display

Key Action

Display panel Displays folders and menu options that guide you through taking

measurements and viewing your results.

Turns the instrument on or off.

Use the four arrows to navigate around the display and select the required setting from the active (highlighted) option.

The Options ke y displays the options for that method or folder. See on page 25 for more information. Options unique to a specific method are described in the relevant section.

The 0A/100%T key sets a reference solution measurement to 0.000 A or 100%T at the current wavelength. When in scan mode, it performs a reference scan.

Options

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Using your Instrument . 21

Alphanumeric keys

The Cancel/Ba c k/Stop key Cancels a selection and returns to the previous folder, goes Back to previous screen in a method or Stops a measurement.

The OK/Next/Run/Scan key, enters or confirms a selection, moves to the Next screen or takes a measurement.

Use these to enter Method Settings, make Options menu selections and to write text descriptions, where appropriate. Use repeated presses to cycle through lower case, number and upper case. Leave for 1 second before entering the next character.

Use the

key to backspace. Press 1 to enter a space.

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Switching on the Instrument

NOTE: If the instrument has just been unpacked or has been stored in a cold environment, it

should be allowed to come to thermal equilibrium for 2–3 hours in the laboratory before being switched on. This will prevent ca lib r ation failure as a result of internal condensation.

Do not turn power to the instrument on and off quickly as this may

CAUTION

 Switch on the instrument via the ON/OFF on the keypad, as shown in Figure 2.

The instrument will perform a series of diagn ost ic che c ks.

NOTE: If during the calibration the instrument disp lays an error message it will prompt you

to press OK to restart the calibration procedure. However, after three attempts you will still be able to view the default home page. You should NOT use the instrument under these circumstances. Instead, make a note of the error message and refer to the Troubleshooting Guide on the PerkinElmer website to address the problem.

After switch on and calibration, the default home page is displayed. To open a method or folder, press the appropriate number on the keypad.

damage the power supply. Wait at least 30 seconds before restarting the instrument.

Figure 3 Lambda Bio home page

NOTE: If you have an SD Memory Card installed in your instrument that contains methods,

the SD Memory Card folder will be added to the home page. You can view and open the first 9 methods saved to this folder. Further methods can be saved to the SD Memory Card folder; see these methods on your PC. If the SD Memory Card is empty it will not be displayed on the home page.

SD Memory Card

on page 29 for information on how to view

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Using your Instrument . 23

Press OK to save Press Cancel to

Using the Software

The home page contains a list of folders and functions or methods. Each item in the list is numbered and is ope ned by using the associated number on the keypad. Opening a folder takes you to another screen of further options. Opening a method takes you to a Method Settings screen where you can set the required parameters before using the method to take measurements.

the selected Method Settings and go on to the next screen

Figure 4 Features of a typical Method Settings screen

Navigation

 Move between settings using the up and do wn arrows.

Entering Method Settings

 Enter Method Settings by using the keypad numbers.

If the box contains the symbol choose an option from the next screen.

If the box contains arrow symbols, use the le ft required setting.

, either type in a value or press options , and

erase selection s and return to the previous screen

and right arrows to select the

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Taking measurements

After the method settings have been entered, press OK to start measurements. The results screen for the method is displayed.

Figure 5 A typical results screen

1. Insert the Reference sample in the sample holder, and press OA/100%T the reference measurement.

2. Insert your first sample and press

3. Repeat Step 2 for each sample.

to take

Results

The results are displayed on screen.

NOTE: Results shown as ---- indicate that the concentration is out of range, for example, if a

negative absorbance value is obtained.

Figure 6 The Single Wavelength me th od results screen

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Using your Instrument . 25

Options

Within each method you are able to select various options that define the way results are treated. These options may be specific to the method, allow you to print the results or view method settings.

 Press Options

The options are:

Option Text Function

1 Method Settings Returns to the Method Settings screen for the method.

2 Print Prints the results.

3–6 N/A Specific to the method. 7 Sample Number Defines the sample number you wis h to start taking

to view the Options menu.

measurements from.

8 Save Method Allows you to save the Method Settings as a new method to

the Methods or Favorites folders or, if installed, to an S D Memory Card. You can save up to 9 methods in each folder.

NOTE: Methods from more than one type of instrument can

be saved to the same SD Memory Card. The Methods are saved to the c ard in a default directory that depends on the instrument type: \<Instrument type>\Methods. For example, \Lambda XLS\Methods

9 Auto-Print Toggles Auto-Print on and off. The default is off.

 Press or , or wait 20 seconds to exit the method options.

You can use the numeric keys as a shortcut to the option required without needing to enter the Options menu.

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26 . Lambda XLS/XLS+ User's Guide

If you are not using a previously stored method, it is advisable to check that these options have been appropriately set for your experiment before running your samples.

NOTE: Setting the History method setting to On (see

the instrument to store its last settings. If the History method setting is Off, all method settings and options will return to their default s when you leave t hat method.

Preferences

on page 36) will cause

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Using your Instrument . 27

Methods and Favorites

The Methods folder allows you to store user-modified methods, based on the default instrument methods, which can be saved using the Options menu. You can save up to 9 methods in each folder. To retrieve previously stored methods, press the appropriate keypad number to enter the Methods folder. You can also export (backup) methods to an SD card or import (restore) methods from an SD Memory Card.

Figure 7 Methods folder

Folder Options

 Press to display the available Options in the Methods folder, which you can select

using the keypad numbers:

Option Text Function

1 Folder Names Allows you to rename the Methods folders.

2 Lock Folder Allows you to lock the folder.

3 Unlock Folder Allows you to unlock the folder.

4 SD Memory

Card

Allows you to access options for the SD card. (This option is only available if you have an SD installed in the instrument.)

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Folder Names

1. Open the Methods folder.

2. Press

3. Press 1 to select Folder Names.

4. Select the Folder you wish to rename using the left and right arrows, and then press the down arrow.

5. Enter a New Name for the folder using the alphanumeric keypad.

6. Press OK

to display the available Options.

to rename the folder.

Lock Folder

1. Open the Methods folder.

2. Press

3. Press 2 to select Lock Folder.

4. Select the Folder you wish to lock using the left and right arrows, and then press the down arrow.

5. Enter a Pass Code for the folder using the keypad numbers or left and right arrows.

to display the available Options.

6. Press Lock

to lock the folder.

Unlock Folder

1. Open the Methods folder.

2. Press

3. Press 3 to select Unlock Folder.

4. Select the Folder you wish to unlock using the left and right arrows, and then press the down arrow.

5. Enter the Pass Code for the folder using the keypad numbers or left and right arrows.

6. Press Unlock

to display the available Options.

to unlock the folder.

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Using your Instrument . 29

SD Memory Card

If an SD Memory Card is installed in your instrument, then you can access the SD Memory Card folder option. The options Backup Folder or Backup All Folders allow you to copy a methods folder or all of the methods folders on your instrument, respectively, to the SD Memory Card. Methods from more than one instrument can be saved to the same card. The methods are saved to a default directory \<Serial Num ber >\BACKUP

For example, \1000\BACKUP\METHODS\M et hods 1 The SD Memory Card can be plugged into a card reader attached to your PC. Methods can be

renamed (up to a maximum of 24 characters) and moved between folders. The options Restore Folder or Restore All Folders restore the contents of the folder or

folders, respectively, to the content of the SD Memory Card BACKUP directory. All methods in the folder(s) will be overwritten if you select Restore Folder or Restore All Folders. If you have renamed an instrument folder, the folder name will be overwritten by those in the BACKUP directory of the SD Memory Card.

NOTE: If you rename a folder on the SD Memory Card and then try to restore the contents,

your instrument will not recognize the folder and it will not be restored.

The Restore All Folders function can also be used if you wish to set multiple instruments of the same type to have the same methods and folder structure. Navigate in Windows Explorer to the \<Serial Number>\BACKUP folder that contains the methods you would like to use and then rename the folder \<Instrument Type>\BACKUP. You can then restore e ach ins trum ent in the normal way.

To Backup or Restore your folders:

1. Open the Methods folder.

2. Press

3. Press 4 to select SD Memory Card. The SD Memory Card screen is displayed.

to display the available Options.

4. Select the mode of Operation using the left and right arrows, and then press the down arrow.

The options are Backup Folder, Backup All Folders, Restore Folder or Restore All Folders.

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5. Select the Folder using the left and rig ht arrows.

6. Press OK

NOTE: The LED next to the card will be lit while methods are saved to the card. The card

should not be removed from the instrument while the LED is lit as this could corrupt your data.

Method Options

 Press to display the available Options in the Methods folder, which you can select

using the keypad numbers:

Option Text Function

1 Delete Method Allows you to delete the Method. 2 Lock Method Allows you to lock the method.

3 Unlock Method Allows you to unlock the method.

Delete Method

1. Open the appropriate methods folder.

2. Press

3. Press 1 to select Delete Method.

4. Select the method to be deleted using the left and right arrows.

5. Press Delete

to display the available Options.

to delete the method.

Lock Method

1. Open the appropriate methods folder.

2. Press

3. Press 2 to select Lock Method.

4. Select the method to be locked using the left and right arrows, and then press the down arrow.

5. Select a Pass Code using the keypad numbers or left and right arrows.

to display the available Options.

6. Press Lock

to lock the method.

Page 31

Unlock Method

1. Open the appropriate methods folder.

Using your Instrument . 31

2. Press

3. Press 3 to select Unlock Method.

4. Select the method to be unlocked using the left and right arrows, and then press the down arrow.

5. Enter a Pass Code using the keypad numbers or le ft and right arrows.

6. Press Unlock

to display the available Options.

to unlock the method.

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32 . Lambda XLS/XLS+ User's Guide

Lambda XLS/XLS+ Instrument Methods

The pre-defined methods available on your Lambda XLS/XLS+ instrument are:

• Single Wavelength

• Multi Wavelength

• Spectrum

• Concentration

• Standard Curve

• Kinetics

• Absorbance Ratio.

Figure 8 Lambda XLS home page

NOTE: If you have an SD Memory Card installed in your instrument that contains methods,

the SD Memory Card folder will be added to the home page.

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Using your Instrument . 33

Utilities

You can use the Utilities folder to modify the instrument setup.  From the home page, press the appropriate keypad number to enter the Utilities folder.

The Utilities screen is displayed.

Figure 9 The Utilities screen

The Utilities are:

1 Date and Time Set correct time and date.

2 Regional Select preferred language and number format.

3 Printer Printer/output options.

4 Preferences Select screen layout (themes) and history. 5 Contrast Adjust screen contrast and brightness.

6 About Serial number and software version.

7 Games Spectro Blocks/Sudoku.

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34 . Lambda XLS/XLS+ User's Guide

Date and Time

1. Press 1 to select Date and Time. The Date and Time screen is displayed.

2. Using the keypad numbers or left and right arrows, enter the Day, Month, Year, Hour and Minute.

3. Press OK folder.

Seconds are zeroed when you press OK.

to store the settings to store the settings and return to the Utilities

Regional

1. Press 2 to select Regional. The Regional screen is displayed.

2. Using the left and right arrows, select a Language and then press the down arrow. The options are Chinese, English, French, German, Italian, Japanese or Spanish.

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Using your Instrument . 35

3. Select the Number Format (the type of decimal separator) using the left and right arrows. The options are “,” o r “.”.

4. Press OK

to store the settings and return to the Utilities folder.

Printer

1. Press 3 to select Printer. The Printer screen is displayed.

2. Select whether Auto-Print is On or Off using the left and right arrows, and then press the down arrow.

When Auto-Print is On, the results are automatically printed after a measurement is taken or when you exit the application, depending on the method. The appro pri at e icon for yo ur Printer option will be displayed in the top right corner of the method results screen.

When Auto-Print is Off, printing has to be initiated manually.

3. Select how the data are sent. The options are Built-in (the internal printer), Computer (USB) (to a computer via USB

port), SD Memory Card or Computer (Bluetooth) (to a computer via Bluetooth). The options available depend on what is installed in your instrument.

NOTE: If SD Memory Card is selected, data is saved as .pvc files in a folder that depends

upon the instrument serial number: \<Serial number >\PVC. The method type is saved in the file name. For information on viewing your results and savi ng your data into other formats, see “Lambda Bio XLS Report Utility Viewer” in the

Lambda Bio/XLS Print Utility User’s Guide

NOTE: If an SD Memory Card is installed in your instrument, the LED next to the card will be

lit while data is being printed to the card. The card should not be removed from the instrument while the LED is lit as this could corrupt your data. For some methods you have to exit the method to end printing. See the “Receiving Pri nt Data” in the

Lambda Bio/XLS Print Utility User’s Guide

4. Press OK to store the settings and return to the Utilities folder.

(L6050015).

(L6050015) for more information.

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36 . Lambda XLS/XLS+ User's Guide

Preferences

1. Press 4 to select Preferences. The Preferences screen is displayed.

2. Select whether Games are turned available using the left and right arrow keys. The options are Yes or No.

3. Define the Theme (the on-screen layout of folders). The options are either a Grid format or a List.

4. Select the History mode using the left and right arrow keys. If Off is selected the instrument will not remember your method settings unless you

restore them as a new method. The default is Off.

5. Select whether to use an Auto Standby mode after defined periods. The options are 1 hour, 2 hours, At Night or Off.

6. Press OK

to store the settings and return to the Utilities folder.

Page 37

Using your Instrument . 37

Contrast

Ambient temperature can affect the display. The contrast function can optimize the display for local conditions.

The procedure is as follows:

1. Press 5 to select Contrast. The Contrast screen is displayed.

2. Adjust the Brightness using the left and right arrows, and then press the down arrow.

3. Adjust the Contrast using the left and right arrows.

4. Press OK

to store the settings and return to the Utilities folder.

About

The About screen displays the instrument serial number and the software version.

1. Press 6 to select About. The About screen for your instrument is displayed.

2. Press OK

to close the window and return to the Utilities folder.

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38 . Lambda XLS/XLS+ User's Guide

Sample Handling

• The light beam is directed from RIGHT to LEFT through the cell chamber, as indicated by the white arrows on the instrument enclosure. Make sure that the cell is inserted in the correct alignment.

• The cell holder supplied with the instrument accepts standard 10–40mm (Lambda XLS) or 10 mm (Lambda XLS+) pathlength quartz, glass or plastic cells.

• The optical height is 15 mm, and the minimum volume that can be used is approximately 70 µl in a micro cell.

NOTE: When using calibration standard filters, insert such that the flat surface is facing

away from the spring end of the cell holder.

NOTE: A cover is provided to keep the sample compartment free from dust and debris.

When the instrument is in use, the d ust cover should be kept in a safe place, nearby.

Page 39

Instrumen t Methods

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40 . Lambda XLS/XLS+ User's Guide

Single Wavelength

The Single Wavelength method makes absorbance (A) and percentage transmission (%T) measurements on samples by measuring the amount of light that has p assed through a sample relative to a reference (this can be air).

The procedure is as follows:

1. From the home page, press 1 to open the Standard Methods folder. The Standard Methods screen is displayed.

2. Press 1 to open the Single Wavelength method. The Single Wavelength – Method Settings screen is displayed.

3. Set the Wavelength by using the keypad numbers or the left and right arrows, and then press the down arrow.

4. Select the Mode, using the left and right arrows. The options are Absorbance or %Transmission.

Page 41

5. Press OK to start measurements. The Single Wavelength results screen is displayed.

Instrument Methods . 41

6. Insert the reference sample and press This reference measurement will be used for all subsequent samples until repeated.

7. Insert your first sample and press The result at the selected wavelength is displayed on screen. Use the left and right

arrows to move the cursor and display the va lue at the cursor position (±20 nm from the set wavelength).

8. Repeat Step 7 for each sample.

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42 . Lambda XLS/XLS+ User's Guide

 Press to display the available Options, which you can select using the keypad

numbers:

1 Method Settings Returns to the Method Settings screen.

2 Print Prints the result using the printer selected in the Utilities folder.

3 A/%T Toggles between Absorbance and %Transmission mode. 4 Print Graph Prints the graph (this option is grayed out if no data are available).

7 Sample Number Adds a prefix to the sample number and resets the incrementing

number to the desired value.

8 Save Method Use the left and right arrows to select the folder you want to store the

method in (Favorites/Methods 1–9/SD Mem ory Card), then press the down arrow and enter a name for the method. Press Save

the method.

9 Auto-Print Toggles Auto-Print on and off.

 Press or , or wait 20 seconds to exit the method options.

 Press

measured.

to return to the Standard Methods folder after all your samples have been

to save

Page 43

Instrument Methods . 43

Multi Wavelength

The Multi Wavelength method can provide absorbance readings at up to 5 wavelengths. The procedure is as follows:

1. From the home page, press 1 to open the Standard Methods folder. The Standard Methods screen is displayed.

2. Press 2 to open the Multi Wavelength method. The Multi Wavele ngth – Method Settings screen is displayed.

3. Select the number of Wavelengths and then press the down arrow. The options are between 2 and 5.

4. Enter the first wavelength (λ1) using either the number keys or the left and right arrows, and then press the down arrow.

5. Repeat Step 4 for each of the wavelengths.

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44 . Lambda XLS/XLS+ User's Guide

6. Press OK to start measurements. The Multi Wavele ngth results screen is displayed.

7. Insert the Reference sample and press This reference measurement will be used for all subsequent samples until repeated.

8. Insert your first sample and press A spectrum covering the range of wavelengths is di splayed, with cursors marking the

chosen wavelengths. A table of the absorbance values at those wavelengths is also displayed.

9. Repeat Step 8 for each sample.

Page 45

Instrument Methods . 45

 Press to display the available Options, which you can select using the keypad

numbers:

1 Method Settings Returns to the Method Settings screen.

2 Print Prints the data on the results screen (the absorbance table) us ing the

printer selected in the Utilities folder.

4 Print Graph Prints the graph (this option is grayed out if no data are available).

7 Sample Number Adds a prefix to the sample number and resets the incrementing

number to the desired value.

8 Save Method Use the left and right arrows to select the folder you want to store

the method in (Favorites/Methods 1–9/SD Memory Card), then press the down arrow and enter a name for the method. Press Save

to save the method.

9 Auto-Print Toggles Auto-Print on and off.

 Press or , or wait 20 seconds to exit the method options.

 Press

measured.

to return to the Standard Methods folder after all your samples have been

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46 . Lambda XLS/XLS+ User's Guide

Spectrum

The Spectrum method enables you to collect an absorption spectrum, and determine peak heights and positions.

The procedure is as follows:

1. From the home page, press 1 to open the Standard Methods folder. The Standard Methods screen is displayed.

2. Press 3 to open the Spectrum method. The Spectrum – Method Settings screen is displayed.

3. Set the Start Wavelength by using the keypad numbers or the left and right arrows, and then press the down arrow.

4. Set the End Wavelength by using the keypad numbers or the left and right arrows, and then press the down arrow.

Page 47

Instrument Methods . 47

5. Select the Mode using the left and right arrows, and then press the down arrow. The options are Absorbance or %Transmission.

6. Press OK The Spectrum results screen is displayed.

7. Insert the Reference sample and press This reference measurement will be used for all subsequent samples until repeated.

8. Insert your first sample and press A spectrum and a table of the Absorbance/%Transmission values at each peak are

displayed. Use the left and rig ht arrows to move the cursor along the graph. When it reaches a

peak, the peak height and width are displayed at the top of the screen. To zoom in on the wavelength scale, use the up arrow key. (This automatically scales the Absorbance/%Transmission axis, depending upon the Graph Scale option, and this is retained for subsequent measurements.) To zoom out again, use the down arrow key.

to start measurements.

9. Repeat Step 8 for each sample.

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48 . Lambda XLS/XLS+ User's Guide

 Press to cancel the selections; pre ss to accept them.

 Press to display the available Options, which you can se lect using the keypad

numbers:

1 Method Settings Returns to the Method Settings screen.

2 Print Prints the result using the printer selected in the Utilities folder.

3 A/%T Toggles between Absorbance and %Transmission mode 4 Peak Detection Displays the Peak Detection settings screen.

Auto-detect Peaks : Tur ns on and off the automatic peak detection.

The following options determine how peaks are detected:

• Min Peak Height: Minimum height the peak has to be above the higher of the two adjacent minima for the peak to be detected.

• Min Peak Width: Minimum width of the peak as determined by the difference in wavelength between the higher of the two adjacent minima and the opposing intersection of that higher minimum level and the peak profile.

• Peak Detect on Zoom: Determines if peaks are reassessed and tabulated when you zoom into a region of the spectrum. If No is selected, the peak detection remains as determined before zooming.

• Sort Peaks By: Determines the sequence that peaks are reported by. The options are Peak Width, Wavelength and Peak Height.

• Indicate Peaks: Switches display of peak cursors on and off. The peak cursors show vertical dashed lines displaying the measured peak height and horizontal dashed lines showing the peak width.

Page 49

Instrument Methods . 49

5 Add Peak/Delete

Peak

Add Peak manually adds a peak position to the peak table in the results screen at the position set by the cursor. The entry in the peak table is then displayed in inverse shading to discriminate between User Defined Peaks and Auto-Detect peaks. If the cursor is positioned over a user-defined peak, the legend User Defined Peak is displayed at the top of the scan. The option then changes to Delete Peak to enable you to remove the peak.

NOTE: Saving a method at t his stage will save these user-defined

wavelengths, so that each time the method is run the Absorbance values at these wavelengths are reported.

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50 . Lambda XLS/XLS+ User's Guide

and display the required graph.

6 Graph Scale Displays the Graph Scale settings screen. This enables you to set

up a defined graph by defining the limi ts in either or both of the

axes.

and

Zoom Mode: This sets up the operation of the Zoom keys (up and down arrows). The mode x axis zooms the display around the cursor point x axis, the mod e y axis zooms the display around the cursor point y axis, and x & y axes expands both axes about the

cursor point. With be permitted within the set limits.

or y axis limits set to On, zooming out will only

x axis limits/y axis limits: Setting to On activates the start and finish points of the desired graph to user-defined, specific wavelengths and/or absorbance values.

 Press

7 Sample Number Adds a prefix to the sample number and resets the incrementing

number to the desired value.

8 Save Method Use the left and right arrows to select the folder you want to store

the method in (Favorites/Methods 1–9/SD Memory Card), then press the down arrow and enter a name for the method. Press

Save

9 Auto-print Toggles Auto-Print on and off.

 Press or , or wait 20 seco nds to exit the method options.

 Press

measured.

to return to the Standard Methods folder after all your samples have been

to cancel the selections; press to accept them

to save the method.

Page 51

Instrument Methods . 51

Concentration

The Concentrati on method makes simple concentration measurements on samples by measuring the amount of light that has passed through a sample relative to a reference (this can be air). The concentration is determined by measuring the absorbance at a specific wavelength and then multiplying by a factor. If the factor is known in advance it can be entered into the method. If the facto r is not known, a standard of known concentration can be measured and the factor then calculated by the instrument.

The procedure is as follows:

1. From the home page, press 1 to open the Standard Methods folder. The Standard Methods screen is displayed.

2. Press 4 to open the Co ncentration method. The Concentration – Method Settings screen is displayed.

3. Enter the Wavelength by using the keypad numbers or the left and right arrows, and then press the down arrow.

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52 . Lambda XLS/XLS+ User's Guide

4. Select the Mode using the left and right arrows, and then press the down arrow. The options are Enter Factor (where a known factor i s entered), Measure Standard

(where the factor is calculated from a calibration sample) or Enter Neg. Factor (where a known negative factor is entered).

5. If you selected Enter Factor or Enter Neg. Factor, enter the Factor (or Negative Factor) using the keypad numbers, and then press the down arrow.

The available range is from 0.01 to 9999. OR If you selected Measure Standard, enter the Concentration using the keypad

numbers and then press the down arrow. The available range is from 0.001 to 9999.

6. Enter the Units and then press the down arrow. You can enter text up to 8 characters long using the keypad, or press Options

display the Units screen and access a list of pre-defined units .

In the Units scree n, use the left and right arrows to select from the available options. The options are µg/ml, µg/µl, pmol/µl, mg/dl, mmol/l, µmol/l, g/l, mg/l, µg/l, U/l, %, ppm, ppb, conc or none.

The Units screen also enables you to select the number of displayed decimal points (DP). The options are Auto, 0, 1 and 2.

NOTE: The result will always be fixed to 5 significant figures, regardless of how ma ny

decimal points are selected (so 98768.2 is displayed as 98768, even with 1 decimal point selected).

Press OK to save your options and return to the Method Settings screen. You can still edit the units.

Page 53

7. Press OK to start measurements. The Concentration results screen is displayed.

Instrument Methods . 53

8. Insert the Reference sample and press This reference measurement will be used for all subsequent samples until repeated.

9. If you selected Measure Standar d, insert your standard and press OK The Run Standard screen is displayed.

OR If you selected Factor or Negative Factor, go to Step 11.

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54 . Lambda XLS/XLS+ User's Guide

10. Press Run to run the standard. The Factor is calculated and displayed.

11. Insert your first sample and press The Concentration of the sam p le is displayed.

12. Repeat Step 11 for each sample.

Page 55

Instrument Methods . 55

 Press to display the available Options, which you can select using the keypad

numbers:

1 Method Settings Returns to the Method Settings screen.

2 Print Prints the result using the pri nter selected in the Utilities

folder.

3 Show Graph Toggles the graph on and off. The wave length range of the

graph displayed is ±20 nm from the selected wavelength, which is marked by the cursor.

4 Run Standard Returns to the Run Sta ndard screen.

7 Sample Number Adds a prefix to the sample number and resets the

incrementing number to the desired value.

8 Save Method Use the left and right arrows to select the folder you want to

store the method in (Favorites/Methods 1–9/SD Memory Card), then press the down arrow and enter a name for the

method. Press Save

9 Auto-Print Toggles Auto-Print on and off.

 Press or , or wait 20 seconds to exit the method options.

 Press

measured.

to return to the Standard Methods folder after all your samples have been

to save the method.

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56 . Lambda XLS/XLS+ User's Guide

Standard Curve

This method enables the construction of a multi-point calibration curve from standards of known concentration that can then be used to quantify unknown samples. This instrument has the advantage of being able to store this curve as a method, using up to nine standards.

If you wish to have a zero concentration standard, include this in the number of standards to be entered and enter 0.00 for the Concentration, then use a reagent blank when required to enter the zero standard.

The procedure is as follows:

1. From the home page, press 1 to open the Standard Methods folder. The Standard Methods screen is displayed.

2. Press 5 to open the Standard Curve method. The Standard Curve – Method Settings screen is displayed.

3. Enter the required Wavelength using the keypad numbers or left and right arrows, and then press the down arrow.

Page 57

Instrument Methods . 57

4. Enter the number of Standards to be use d in the standards curve using the keypad numbers or left and right arrows, and then press the down arrow.

The options are 1–9.

5. Enter the Units and then press the down arrow. You can enter text up to 8 characters long using the keypad, or press Options

display the Units screen and access a list of pre-defined units.

The Units screen also enables you to select the number of displayed decimal points (DP). The options are Auto, 0, 1 and 2.

NOTE: The result will always be fixed to 5 significant figures, regardless of how ma ny

decimal points are selected (so 98768.2 is displayed as 98768, even with 1 decimal point selected).

Press OK to save your options and return to the Method Settings screen. You can still edit the units.

6. Select the type of Curve Fit using the left and right arrows, and then press the down arrow.

The options are Zero Regression (thi s forces the straight line through the origin),

Regression (straight-line regression), Interpolation (linear interpolation) or Cubic Spline.

7. Select the Calibration mode using the left and right arro ws. The options are Standards (to measure prepared standards) or Manual (entering

absorbance values by keypad) or New Standards (to replace any standards that have already been collected).

8. If you selected Standards, press the down arrow and enter the number of Replicates. This determines the number of standards to be measured and averaged at each

standard concentr ation point. The options are Off (1), 2 or 3.

NOTE: If you selected Manual in Step 7, the option Replicates is not available.

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58 . Lambda XLS/XLS+ User's Guide

9. Press Next . The Standard Curve – Standards screen is displayed.

10. Enter the concentration values for each standard using the keypad numbers and the up and down arrows to move be tween the different standard boxes.

The available range is from 0.001 to 9999.

11. Press Next The Standard Curve – Calibration screen is displayed.

NOTE: If any dupli c ate or non-monotonic entries are entered on the Standards screen, when

Next is pressed the instrument will beep and highlight the incorrect entry.

Page 59

Instrument Methods . 59

12. If you selected Standards, insert the reference sample and then press . This reference measurement will be used for all subsequent samples until repeated.

OR If you selected Manual, enter the known absorbance value for each concentration using

the keypad. Go to Step 15. The available range is from 0.001 to 9999.

13. Insert your first standard and press If there are any replicates, press Replicates

measure each replicate.

14. Repeat Step 13 for all your standards. A graph will display the results and the curve fit (for example, zero regression) a s the

measurements are made.

to view them and then press to

NOTE: You can use the up and down arrows to select a standard to be repeated. Press

to clear the previous reading before measuring.

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60 . Lambda XLS/XLS+ User's Guide

If you selected Replicates, then when all replicates have been measured press Next

to return to the Standards screen.

15. Press OK The Standard Curve results screen is displayed.

16. If you selected Manual, insert the reference sample and then press This reference measurement will be used for all subsequent samples until repeated.

to accept the calibration.

If you selected Standards, go to Step 17.

Page 61

Instrument Methods . 61

17. Insert your first sample and then press . The absorbance of the sample is measured, and its concentra tion is calculated and

displayed.

18. Repeat Step 17 for each sample.

 Press

keypad numbers:

1 Method Settings Returns to the Method Settings screen. 2 Print Prints the data on the results scre en using the printer selected in

3 Show Graph Toggles the calibration graph on and off. The cursors display the

7 Sample Number Adds a prefix to the sample number and resets the incrementing

8 Save Method Use the left and right arrows to select the folder you want to store

9 Auto-print Toggles Auto-Print on and off.

to display the available method options, which you can select us ing the

the Utilities folder.

absorbance and corresponding concentration values of the last measured sample.

number to the desired value.

the method in (Favorites/Methods 1–9/SD Memory Card), then press the down arrow and enter a name for the method. Press

Save

to save the method.

 Press or , or wait 20 seconds to exit the method options.

 Press

measured.

to return to the Standard Methods folder after all your samples have been

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62 . Lambda XLS/XLS+ User's Guide

Kinetics

Kinetics studies, where the change in absorbance needs to be followed as a function of time at a fixed wavelength, can readily be performed using the Kinetics method.

Reagent test kits are routinely used for the enzymatic determination of compounds in food, beverage and clinical laboratories by measuring NAD/NADH conversion at 340 n m. The change in absorbance over a specified time period can be used to provide useful information when an appropriate factor, defined in the reagent -kit protocol, is applied. Reaction rate and enzyme activity can be calculated if the fa c tor used takes account of the absorbance difference per unit time, as opposed to only the absorbance difference.

For this reason, the change in absorbance per minute (∆A/min), concentration (∆A/min x

factor) and correlation coefficient (calculated from a best fit of the data points) are displayed. They may not be relevant for simple kinetics experiments.

The procedure to define a new method is as follows:

1. From the home page, press 1 to open the Standard Methods folder. The Standard Methods screen is displayed.

Page 63

Instrument Methods . 63

2. Press 6 to open the Kinetics method. The Kinetics – Metho d Set ti ngs 1 screen is displayed.

Enter all numerical values using the keypad numbers or the left and right arrows.

3. Set the Wavelength by using the keypad numbers or left and right arrows, and then press the down arrow.

The default value is 340 nm.

4. Enter the Delay Time in seconds before measurements are taken and then press the down arrow.

This can be a maximum of 600 seconds (10 minutes).

5. Enter the time in minutes over which measurements are taken (Duration) and then press the down arrow.

This can be a maximum of 60 minutes.

6. Enter the Interval time in seconds between measurements using the left and right arrows, and then press the down arrow.

The options are 1, 2, 5, 10, 15, 20, 30 or 60 seconds.

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64 . Lambda XLS/XLS+ User's Guide

7. Press Next . The Kinetics – Method Setti ngs 2 scr ee n is displayed.

8. Select the measurement Mode using the left and right arrows, and then press the down arrow.

The options are Delta A (the change in absorbance over the measurement duration, or selected period), Final A (the absorbance at the end of the measurement duration, or selected time) and Slope (the rate of change of absorbance over the measurement duration or selected period).

9. Enter the Units and then press the down arrow. You can enter a text string up to 8 characters long. To access a list of pre-defined uni ts

on the Units screen, press Options µg/µl, pmol/µl, mg/dl, mmol/l, µmol/l, g/l, mg/l, µg/l, U/l, %, ppm, ppb, conc or none). These units can also be edited once OK is pressed.

The Units screen also enables you to select the number of displayed decimal points (DP). The options are Auto, 0, 1 and 2.

NOTE: The result will always be fixed to 5 significant figures, regardless of how many

decimal points are selected (so 98768.2 is displayed as 98768, even with 1 decimal point selected).

and then use the left/right arrows (µg/ml,

Press OK You can still edit the units.

to save your options and return to the Method Setti ngs screen.

Page 65

Instrument Methods . 65

10. Set the Factor by which the absorbance is multiplied to give the concentration using the left and right arrows.

The available range is from 0.01 to 9999.

11. Press OK

to start measurements.

The Kinetics results screen is displayed.

12. Insert the reference sample and press

This reference measurement will be used for all subsequent samples until repeated.

13. Insert your first sample and then press

to start the run.

The running Time is displayed at the bottom of the screen, and absorbance data are plotted on the graph as the measurement proceed.

The table below the graph displays A

the absorbance value at tn (finish of calculation); dA, the change in absorbance; the

Slope and the regression parameter (R

the absorbance value at t0 (start of calculation);

) of the calculated slope. The concentration is a calculated using the parameters selected in Method Settings 2 (dA, Final A or Slope) – the name of the Result table column depends on the units chosen in Step 9.

 Use the left and right arrows to move the cursor and display the time and absorbance

value at measured data points.

 Use the up and down arrows to zoom in or out.

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66 . Lambda XLS/XLS+ User's Guide

 Press to display the available method options, which you can select using the

keypad numbers:

1 Method Settings Returns to the Method Settings screen.

2 Print Prints the data on the results scre en using the printer selected in

the Utilities folder.

3 Print Data Prints all the dat a.

4 Set t0 at cursor Sets the t0 position (the starting point for the slope and dA

calculations) at the current cursor positio n. The value is retained for subsequent samples.

5 Set tn At Cursor Sets the tn position (the finishing point for t he slope and dA

calculations) at the current cursor position. The value is retained for subsequent samples.

6 Show Slope Toggles the calculated slope line on and off.

NOTE: If any data points enclosed by t0 and tn are beyond the

range of the instrument (that is, >2.5 A or <−0.3 A) then this option is grayed out.

7 Sample Number Adds a prefix to the sample number and resets the incrementing

number to the desired value.

8 Save Method Use the left and right arrows to select the folder you want to store

the method in (Favorites/Methods 1–9/SD Memory Card), then press the down arrow and enter a name for the method. Press

Save

9 Auto-print Toggles Auto-Print on and off.

 Press or , or wait 20 seconds to exit the method options.

 Press

measured.

to return to the Standard Methods folder after all your samples have been

to save the method.

Page 67

Instrument Methods . 67

Absorbance Ratio

The Absorbance Ratio method makes simple absorbance ratio measurements on samples, by measuring the amount of light that has passed through a sample relative to a blank (this can be air) at two wavelengths.

The procedure is as follows:

1. From the home page, press 1 to open the Standard Methods folder. The Standard Methods screen is displayed.

2. Press 7 to select the Absorbance Ratio method. The Absorbance Ratio – Wavelengths screen is displayed.

3. Enter Wavelength 1 by using the keypad numbers or the left and right arrows, and then press the down arrow.

4. Enter Wavelength 2 as above and then press the down arrow.

5. Select whether a Background correction is applied to both wavelengths 1 and 2 using the left and right arrows.

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68 . Lambda XLS/XLS+ User's Guide

6. If background correction is On, enter Wavelength 3, from which the background correction will be obtained.

7. Press Next The Absorbance Ratio – Method Settings screen is displayed.

8. Select the Pathlength using the left and right ar rows, and then press the down arrow. The options are 5 mm or 10 mm.

9. If the Dilution Factor is known, enter a dilution factor by using the keypad numbers, press the down arrow and then go to Step 13.

The available range is from 1.00 to 9999.

If the dilution factor is unknown, press Options screen.

10. Enter the Volume of the sample using the keypad numbers and then press the down arrow.

The available range is from 0.01 to 9999.

to display the Dilution Factor

Page 69

11. Enter the volume of Diluent by using the keypad numbers. The available range is from 0.01 to 9999.

Instrument Methods . 69

12. Press OK

13. Select the Units of measurement using the left and right arrows, and then press the down arrow.

The options are µg/ml, ng/µl, µg/µl.

14. Enter the Factor using the keypad numbers and then press the down arrow. The available range is from 0.001 to 9999 .

15. Press OK The Absorbance Ratio results screen is displayed.

to calculate the dilution factor and return to the Method Settings screen.

to start measurements.

16. Insert the reference sample and then press This reference measurement will be used for all subsequent samples until repeated.

17. Insert your first sample and press The absorbance at each wavelength is measured. If Background was selected, the

background absorbance is then subtracted from the absorbance at Wavelength 1 and Wavelength 2. The ratio between Wavelength 1 and Wavelength 2 is calculated.

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70 . Lambda XLS/XLS+ User's Guide

18. Repeat Step 17 for each sample.

 Press

keypad numbers:

1 Method Settings Returns to the Method Settings screen.

2 Print Prints using the printer selected in the Utilities folder.

3 Show Graph Toggles the graph on and off. Th e graph shows a spectrum across

7 Sample Number Adds a prefix to the sample number and resets the incrementing

8 Save Method Use the left and right arrows to select the folder you want to store

9 Auto-Print Toggles Auto-Print on and off.

 Press or , or wait 20 seconds to exit the method options.

to display the available method options, which you ca n select usi ng the

the range of the measured wavelengths, which are marked by cursors.

number to the desired value.

the method in (Favorites/Methods 1–9/SD Memory Card), then press the down arrow and enter a name for the method. Press

Save

to save the method.

 Press

measured.

to return to the Standard Methods folder after all your samples have been

Page 71

Accessories

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72 . Lambda XLS/XLS+ User's Guide

Accessories and Consumables

Supplies, accessories, and replacement parts can be ordered directly from PerkinElmer. See the PerkinElmer website at http://www.perkinelmer.com for more information.

Part Number Accessory/Consumable

L7110230 Lambda Bio/XLS Printer Module

L7110231 Lambda Bio/XLS Print Utility and Cable

L7110232 Lambda Bio/XLS Spare Printer Paper (20 Rolls)

L7110233 Lambda Bio/XLS Bluetooth Accessory

A range of cells and microcells are also availab le from PerkinElmer that are suitable for use with the Lambda XLS/XLS+ instrument.

Page 73

Accessories . 73

Cap head screws

Printer Installation and Configuration

The Printer Accessory (L7110230) is an optional accessory that allows you to print your data as you run your samples, directly from your instrument.

Printer installation

1. Turn off the line power supply and disconnect the power cable.

2. Turn the instrument over and remove the two cap head screws using the 3 mm hexagonal wrench provided .

3. Turn the instrument back over and lift the ac c essory cover vertically upwards to remove. Remove the tie-wrap from the cable.

4. Invert the instrument and replace the cap head screws.

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74 . Lambda XLS/XLS+ User's Guide

Locating bosses Accessory cable

Printer

5. Plug the accessory cable into the printer.

6. Lower the printer onto the locating b osses and push down firmly.

7. Reconnect the power cable.

Printer configuration

1. Switch the instr ument on and then open the Utilities folder.

2. Press 3 to open the Printer folder.

3. Select Built-in as the Printer option using the arrow keys.

4. Press OK

to save the settings.

Page 75

Loading or changing the printer paper

Platen lock released

Platen locked

1. Lift off the paper cover.

2. Lock the platen and turn the knob to feed the paper.

3. Feed in the paper. Sometimes it helps if the platen lock is released.

Accessories . 75

4. Replace the cover.

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76 . Lambda XLS/XLS+ User's Guide

Cap head screws

Bluetooth Accessory Installation

1. Remove the power cable from the instrument.

2. Turn the instrument over and remove the two cap head screws using the 3 mm hexagonal wrench provided .

3. Turn the instrument back over and lift the ac c essory cover vertically upwards to remove it.

4. Remove the tie-wrap from the cable.

Page 77

Accessories . 77

Bluetooth accessory slots

5. Plug the accessory cable into the Bluetooth module.

6. Plug the two lugs on the Bluetooth module into the innermost slots in the base of the case.

7. Lower the accesso ry cover vertically downwards onto the instrument, engaging the PCB in the innermost slots in the accessory cover.

8. Invert the instrument and replace the two cap head screws.

9. Connect the power cable.

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78 . Lambda XLS/XLS+ User's Guide

10. Switch the instrument on and open the Utilities folder.

11. Press 3 to open the Printer folder.

12. Select Computer (Bluetooth) as the Printer option using the arrow keys.

13. Press OK

to save the settings.

Page 79

Accessories . 79

Lambda Bio/XLS Print Utility

There are two software applications comprising the Lambda Bio/XLS Print Utility. The Lambda Bio/XLS Report Utility is an application running separately under Windows XP

that enables you to connect via a USB cable to a PC onto which the software has been installed. You can connect to several instruments simultaneously. Once your data is on the PC it can be printed directly to any printer that is connected to that PC or saved. The Report Utility can save data as an Enhanced Metafile (.emf) graphics file, a comma delimited (.csv) data file, a tab delimited (.txt) data file or as an Excel file, or in print utility (.pvc) format for viewing using the Lambda Bio/XLS Report Viewer.

NOTE: An internal print er is also available for the instrume nt. This is either supplied

pre-installed in the instrument or as an optional accessory (L7110230).

If you have this option, please see the on the

Lambda Bio/XLS User Manuals CD

Lambda Bio/XLS Print Utility User’s Guide

(L6050018) for more information.

(L6050015)

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80 . Lambda XLS/XLS+ User's Guide

Page 81

Maintenance

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82 . Lambda XLS/XLS+ User's Guide

Unauthorized Adjustments and Servicing

General Maintenance

Do not attempt to make adjustments, replacements or repairs to this

WARNING

CAUTION

instrument. There are no user-serviceable parts in this instrument. If you have any problems, access the Troub leshooting Gui de on the PerkinElmer website and work through the process there:

http://www.perkinelmer.com/LambdaBioXLSSupport If you do not have access the Troubleshooting Guide, or have any

further problems, contact Customer Care (see Appendix 2: Customer Care on page 87

Before using any cleaning or decontamination me thods except those specified here, users should check with Customer Care that the proposed method will not damage the instrument.

for more information).

Cleaning the instrument

External Cleaning

Switch off the instrument and disconnect the power cord before cleaning your instrument. Clean the outside of the instrument using a damp cloth. A mild liquid detergent may be used,

if necessary. Always test an inconspicuous area of the instrument before you clean the entire instrument.

Clean all spilled materials from the affected area immediately and wipe it dry with lint-free paper or cloth.

Removing the cell holder for cleaning

To remove the sample cell holder for cleaning:

1. Loosen the thumbscrews on the sample-holder cover on the base of the instrument by turning them anticlockwise.

The thumbscrews remain attached to the plate.

Page 83

2. Lower the sample holder out of the instrument.

Thumbscrews

Figure 10 Position of cell holder thumbscrews

Maintenance . 83

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84 . Lambda XLS/XLS+ User's Guide

Storage and Shipping

This unit should be stored or shipped in its original packaging, which should be kept for this purpose. The unit should be kept in i ts packaging in a dry, non condensing environm ent, free from dust or dirt, in the range −10 to 50 °C.

Should you need to return your instrument to PerkinElmer, a decontamination certificate is required before the instrument is shipped. Decontamination certificates can be obtained from PerkinElmer. The Troubleshooting Guide, which will help you work thr ough the returns process, can be accessed at:

http://www.perkinelmer.com/LambdaBioXLSSupport

If you do not have access to the Troubleshooting Guide, you can also contact Customer Care

Appendix 2: Customer Care

(see It is the responsibility of the user to carry out appropriate risk assessments and, if applicable,

decontaminatio n procedures be fo re returning the instrument.

on page 87 for more information).

Page 85

Appendices

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86 . Lambda XLS/XLS+ User's Guide

Appendix 1: Specifications

Specifications are measured after the instrument has warmed up at a constant ambient temperature and are typical of a production unit.

Specification Lambda XLS Lambda XLS+

Wavelength range 190–1100 nm

Wavelength scanning range 200–950 nm

Monochromator Flat grating

Wavelength calibration Automatic when the instrument is switched on

Spectral bandwidt h 5 nm 3 nm

Wavelength accuracy ±2 nm

Wavelength reproducibility ±1 nm

Light sources Pulsed xenon lamp

Detector 1024 element CCD array

Absorbance range −0.300 to 2.500 A (0 to 199%T)

Photometric linearity ±0.005 A or 1% of the reading, whichever is the greater at 546 nm

Photometric reproducibility ±0.003 A (0–0.5 A), ±0.007 A (0.5–1.0 A)

Stray light <0.5% at 220 nm and 340 nm using NaNO2

Zero stability ±0.01 A/hour after 20 minutes warm up at 340 nm

Noise 0.005 peak-to-peak 0.002 rms

Digital output USB port standard, Bluetooth option

Dimensions 340 × 310 × 170 mm (340 × 420 × 170 mm with printer attached)

Weight Approximately 4.5 kg

Power input 100–250 V, 50/60 Hz, Max 30 VA

Page 87

Appendices . 87

Appendix 2: Customer Care Contacts

NOTE: If you experience any problems with your instrument, please refer to the

Troubleshooting Guide at http://www.perkinelmer.com/LambdaBioXLSSupport before contacting Customer Care.

Customer Care USA: 1 00 762 4000 (In the USA) (+1) 203 925 4602 (Outside USA)

Customer Care EU: 0800 40 858 (Belgium)

0805 111 333 (France)

0800 0 00 66 79 (Germany) 0800 90 66 42 (Italy)

800 099 164 (Spain)

0800 896 046 (UK)

Customer Care Brazil: 11 38 68 6200 Customer Care Canada: 800 561 4646

Customer Care China: 10 5820 8166

Customer Care India: 022 6760 1700

Customer Care Japan: 45 339 5889 Customer Care Singapore: 67799 539

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88 . Lambda XLS/XLS+ User's Guide

Page 89

Index

Appendices . 89

Absorbance Ratio ................................ 67

Accessories ......................................... 72

Add Peak ............................................ 49

Bluetooth Installation .......................... 76

Cleaning the instr ument....................... 82

Concentration ..................................... 51

Consumables ...................................... 72

Contrast ............................................. 37

Conventions

keyboard text .................................... 7

Notes, cautions and warnings ............. 7

on-screen text ................................... 7

text ................................................... 7

Customer Care .................................... 87

Display ............................................... 20

Favorites ............................................ 27

Graph Scale ........................................ 50

Maintenance ....................................... 82

Methods ............................................. 27

Absorbance Ratio ............................. 67

Concentration .................................. 51

Kinetics ........................................... 62

Method folders ................................. 27

Method Options ............................... 30

Multi Wavelength ............................. 43

Single Wavelength ........................... 40

Spectrum......................................... 46

Standard Curve ................................ 56

Multi Wavelength ................................ 43

Options ............................................... 25

Peak Detection .................................... 48

Preferences ......................................... 36

Printer

Configuration ................................... 74

Installation ...................................... 73

Loading Paper.................................. 75

Printer Setup ................................... 35

Report Utility ....................................... 79

Report Viewer ..................................... 79

Installation

Connecting to the Power Supply ....... 17

Unpacking ....................................... 16

Instrument Home Page ........................ 22

Instrument Overview ........................... 20

Keypad ............................................... 20

Kinetics .............................................. 62

Language ........................................... 34

Safety

General Safety ................................. 10

Unpacking and Installation ............... 13

Sample Handling ................................. 38

Shipping ............................................. 84

Single Wavelength ............................... 40

Slope .................................................. 65

Specifications ...................................... 86

Spectrum ............................................ 46

Standard Curve ................................... 56

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90 . Lambda XLS/XLS+ User's Guide

Standard Methods

Absorbance Ratio ............................. 67

Concentration .................................. 51

Kinetics ............................................ 62

Multi Wavelength ............................. 43

Single Wavelength ............................ 40

Spectrum ......................................... 46

Standard Curve ................................ 56

Startup and Shutdown ......................... 22

Storage ............................................... 84

Using the Software .............................. 23

Utilities................................................ 33

Regional .......................................... 34

Zoom Mode ......................................... 50