PerkinElmer Lambda 365 Installation Instructions Manual

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Lambda 365 6-Position Peltier Controlled Cell Changer,

Controller Installation Instructions

This instruction sheet describes the installation of this accessory which is used with the Lambda 365 Spectrophotometer.

NOTE:

Read these instructions before you install this accessory.

Contacting PerkinElmer

Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using the part numbers.

See our website: http://perkinelmer.com PerkinElmer's catalog service offers a full selection of high-quality supplies. To place an order for supplies and many replacement parts, request a free catalog, or ask for

information: If you are located within the U.S., call toll free 1-800-762-4000, 8 a.m. to 8 p.m. EST. Your order will be

shipped promptly, usually within 24 hours. If you are located outside of the U.S., call your local PerkinElmer sales or service office.

Features

• Full software control

• Liquid cooling system gas purging available

• N

Figure 1 6-Position Peltier Controlled Cell Changer, Controller (with heated

reference) [P/N: N4101029] or Peltier Temp Unit (Multi) w/o Reference Heating [P/N: N4101008]

PerkinElmer, 710 Bridgeport Avenue,

Shelton, CT 06484-4794, U.S.A

Produced in the USA.

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Dimensions and Specifications

Peltier Controller

Physical Characteristic Specification

Power 100-240 VAC, 50/60 Hz, 300W, Free Voltage Temperature Range -5 to 100°C (Maximum Internal Temperature) Maximum Ambient Operating Temperature 40°C

Dimensions

Weight 20.0 kg (44 lb) Coolant Volume 2 L N2 Gas Available Liquid Cooling System Magnetic Stirrer Control Available Emergency Power Off Switch Available

310 (W) x 410 (D) x 275 (H) mm (12.2 (W) x 16.1 (D) x 10.8 (H) in)

100 psi (6.9 bar)

6-Position Peltier Controlled Cell Changer (with and without heated reference)

Physical Characteristic Specification

The number of Sample Cells 6 The number of Reference Cell 1 (with or without heated reference)

Dimensions

135 (W) x 384 (D) x 143 (H) mm (5.3 (W) x 15.1 (D) x 5.6 (H) in)

Weight

Temperature Accuracy ±0.15°C Temperature Precision ±0.1°C Temperature Stability ±0.1°C Ramping Time (from 0 to 100°C) 5 minutes

With heated reference: 3.4 Kg (7.5 lb) Without heated reference: 3.1 Kg (6.8 lb)

Safety Warnings

When this label is attached to an instrument it means refer to the manual.

WARNING

See the following figure for the location of warning labels on the back of the instrument:

Lorsque cette étiquette est attachée à un instrument, il est nécessaire de voir le manuel.

There is risk of receiving a fatal electric shock if the fuses are replaced with the power cord connected.

Il y a un risque d’électrocution si les fusibles sont remplacés tandis que le cordon d'alimentation est encore branché.

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Warnin

Warning FUSE

Avertissement FUSE

For continued protection against electrical shock, replace with same type and ratings of fuse.

Avertissement Pour assurer la protection contre le risque de choc électrique, remplacez le fusible seulement avec le même type et la même valeur nominale.

Warnin To prevent electrical shock,

do not open cover. Refer all servicing to qualified personnel.

Avertissement Afin d’éviter tout risque d’électrocution ne pas enlever ce couvercle. Faire appel au service Après-Vente.

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Configuration of Peltier Temp. Control Unit (Multi)

6-Position Peltier Controlled Cell Changer (with heated reference or without heated reference) Peltier Controller Power Cord, 3ea Interface Cable (RS-232 and USB to RS-232), 1each Spare Fuse (AC 250V T5AL), 2ea Temperature Probe, 1ea Magnetic Stirrer, 6ea Coolant Hose, 1ea Coolant, 2ea Waste Basket for Coolant, 1ea Waste Hose for Coolant, 1ea Phillips round head screw with washer, 2ea

Description

6-Position Peltier Controlled Cell Changer (with heated reference or without heated reference)

Figure 2-1 Lambda 365 6-Position Peltier Controlled Cell Changer (with heated

reference)

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Figure 2-2 Lambda 365 6-Position Peltier Controlled Cell Changer (without heated

reference)

a. Interface connector between 6-Position Peltier Controlled Cell Changer and Peltier Controller b. Coolant Inlet/Outlet Quick Coupler c. N

Gas fitting

d. Interface slot for Temperature Probe e. Cell Lifting knob for sample cells f. Cell Lifting knob for reference cell g. Phillips round headscrew with washer

Used to fix a cell holder, a base plate or a front plate for Lambda 365. Spare screws (2ea) are enveloped with the accessory.

Figure 3 Phillips round headscrew with washer (M4 *12L)

h. Temperature Probe

Figure 4 Temperature probe

Peltier Controller

Front View of Peltier Controller

Figure 5 Front view a. Manual touch pad with Display: Temperature Control Unit (Heating/Cooling Block).

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NOTE:

b. LED Indicators: Display the status of the operation of Coolant Circulation, Fan, Pump, etc.

(If there is any problem in the components, Red LED will flash with beep alarm.).

c. Manual ON/OFF button: As the Manual ON/OFF button is ON, the temperature can be

controlled manually by a) Manual touch pad with Display.

Manual operation is used only for the manufacturer so it should be turned off during the measurement.

d. N2 gas ON/OFF button: It is used for using N2 gas to remove the frost from the heating

and cooling. e. Stirrer ON/OFF button: It is used for controlling of the stirring. f. Stirrer Speed Controlling Knob: Control the stirring speed (rpm).

g. Emergency switch: Stop all operations in the emergency situation.

During the operation, Emergency switch should be turned off.

h. Coolant Inlet

Rear View of Peltier Controller

Figure 6 Rear view

a. RS-232 port b. AC Power: Main Power ON/OFF

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NOTE:

c. Fuse: AC socket + Fuse Holder d. Interface: Interface cable for connecting with 6-Position Peltier Controlled Cell Changer e. Air Vent Manual button: It is used for removing the air in the coolant hose. f. Buzzer: It makes an alarm sound. If there is any problem before the measurement, Buzzer

beeps 6 times at every 10 sec. If the malfunction occurs during the operation, Buzzer beeps

twice at every 10 sec. g. Quick Coupler of Coolant lnlet/Outlet h. Quick Coupler of Coolant Drain

Gas Inlet/Outlet ports

i. N

N2 gas is not always required [Tube for N2 gas is not supplied with the accessory].

j. Flow Gauge: Indicator of the flow rate of N

gas.

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Installation

CAUTION

ATTENTION

1. Prepare the Lambda 365 and Peltier Controller in a location that is compatible with the required environmental conditions for the operation.

2. Open the lid of the coolant inlet on top of the Peltier Controller.

Make sure the instrument is turned off while installing this accessory. Assurez-vous que l’instrument est éteint lors de l’installation de cet accessoire.

Figure 7 Lid of coolant inlet

3. Fill up the liquid coolant.

CAUTION

Liquid coolant should be used as refrigerant. Do not use any water (tab water,

DI, etc.). If water is used as refrigerant, the Peltier Controller’s components might be corroded and its performance may be deteriorated.

Use any kind of liquid coolant of normal grade that is available. (e.g. liquid coolant

including Distilled Water 70 wt%, Propylene Glycol 27 wt%, Additives 3 wt%).

ATTENTION

Le liquide de refroidissement doit être utilisé comme réfrigérant. N'utilisez jamais d'eau (eau du robinet, eau déminéralisée, etc.) L'eau risque en effet de corroder les composants du contrôleur Peltier et de réduire les performances de ce dernier.

Utilisez un liquide de refroidissement du commerce de qualité normale (par exemple, un liquide de refroidissement à base d'eau distillée à 70 % M/V, de propylène glycol à 27 % M/V et d'additifs à 3 % M/V).

NOTE:

Keep the amount of liquid coolant to the level where the indicator is located between the ‘L’(low) and

‘H’(high) mark in the scale on the left side of the Peltier Controller. When the coolant is running short for the operation, LED of Low Water Level is changed red with alarm sounding.

4. Connect the power cord and communication cable of the Lambda 365. DO NOT turn on the power of the instrument!

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5. Remove the two Phillips round head screws with washer (M4*12L) to disassemble the existing cell holder and base plate.

Figure 8 Location of Phillips round head screws with washer

6. Pull out the cell holder and base plate by hand.

Figure 9 Pulling out the cell holder and base plate

7. Insert the 6-Position Peltier Controlled Cell Changer in the sample compartment.

Figure 10 Inserting the 6-Position Peltier Controlled Cell Changer

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8. Gently press the 6-Position Peltier Controlled Cell Changer to connect the communication port (male) under the bottom of the 6-Position Peltier Controlled Cell Changer to the port (female) in the sample compartment.

Figure 11. Connecting the communication ports

9. Fix and tighten the 6-Position Peltier Controlled Cell Changer with the Phillips round head screws.

Figure 12 Location of screws (with heated reference or without heated reference)

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10. Connect the accessory interface cable of the Peltier Controller to the interface connector of the 6- Position Peltier Controlled Cell Changer.

Figure 13 Connecting the accessory interface cable of the Peltier Controller

11. Connect the coolant inlet/outlet and N2 gas tube between the Peltier Controller and the 6-Position Peltier Controlled Cell Changer.

NOTE:

Figure 14 Connecting the coolant inlet/outlet and N

gas fitting

N2 gas is not always required. If no gas is applied, user does not have to connect N2 gas tube.

12. Open the lid of Interface slot for Temperature Probe by hand.

Figure 15 Opening the lid of Interface slot

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13. Connect the Temperature Probe to the Interface slot for Temperature Probe.

Figure 16 Connecting the temperature probe

14. Stuff the probe line into the cell compartment and close the lid of the Interface slot for Temperature Probe.

Figure 17 Stuffing the probe line into the cell compartment

15. Connect the Peltier Controller with the PC via the RS-232 or USB to RS-232 cable.

Figure 18 Connecting the Peltier Controller with the PC

16. Connect the power cord to the Peltier Controller.

Figure 19 Connecting the power cord

17. Turn on the AC power switch of the Lambda 365 and the Peltier Controller.

CAUTION

The air vent manual button should be off before turning on the main power of the

Peltier Controller and also during the operation since it could make a trouble for the communication between Lambda 365 and Peltier Controller.

ATTENTION

Le bouton d'actionnement manuel de la ventilation doit être désactivé avant que le contrôleur Peltier ne soit mis sous tension et pendant le fonctionnement de ce dernier afin d'éviter qu'une erreur de communication entre le Lambda 365 et le contrôleur Peltier ne se produise

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Figure 20 Turn on the AC power switch

18. Check that the Power LED is on as a blue light.

Figure 21 Location of Power LED

CAUTION

ATTENTION

If you hear the alarm sound during the operation, push the emergency switch to stop all functions immediately and check the status of Peltier Controller carefully. Then, turn the switch to the right again when everything is solved properly and the functions will be on again.

Si l'alarme se déclenche en cours de fonctionnement, appuyez sur l'interrupteur d'urgence pour arrêter immédiatement toutes les fonctions et contrôlez soigneusement l'état du contrôleur Peltier. Ensuite, tournez à nouveau l'interrupteur vers la droite une fois la situation rétablie afin de restaurer les fonctions

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NOTE:

Figure 22 Location of Emergency Switch

Peltier controller can be compatible with various peltier cell holders (single or Multi). Wwhenever you exchange the existing peltier cell holder to another one, you should perform the “Auto Tuning” setup referring to chapter Peltier Controlled Auto Tuning Setup (p33).

19. Turn off the power after the experiment.

How to Drain the Coolant From the Peltier Controller

a. Push the Air Vent Manual button to be switched on the rear panel of Peltier Controller before

operation.

b. Connect the waste hose to the Coolant Drain port on the rear panel and prepare a waste

bucket.

c. Turn on the power of Peltier Controller, and then coolant will be drained automatically.

d. Once draining is complete, turn off the power of Peltier Controller.

Setting USB to RS-232 Cable

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NOTE:

When using the RS-232 cable, the COM Port is set automatically.

When using the USB to RS-232 cable, USB to RS-232 Driver has already been installed when installing the Lambda 365 and UV Express software, user does not need to install it again.

When the communication by the USB to RS-232 converter is not established, change the port setting as follows;

1. Select My Computer →Properties.

2. Select Device Manager.

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3. Select Ports (COM & LPT) to expand the listing. These are the devices currently connected to the COM ports. USB Serial Port (COMx) is listed when the driver installation is completed successfully.

4. Double click on the USB Serial Port (COMx) of the Ports (COM & LPT).

5. Select the Port Settings tab and select the Advanced… button.

6. Change the parameter values as shown below.

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7. Select OK after checking the changed parameter values.

8. If the Peltier controller fails to communicate with the PC, change the COM Port Number by the following steps.

9. Open Advanced Setting for COMx window again by repeating the steps 1 to 6.

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10. Select the COM Port number list to expand it and change the COM port number to another one which is not in use from COM 1 to COM 10.

11. Make sure that the changed COM Port Number is applied, and select OK.

12. After the port setting is changed, restart the computer.

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Measurement

Thermal Denaturation Mode

1. Install the 6-Position Peltier Controlled Cell Changer referring to the chapter Installation.

2. Close the sample compartment cover and turn on the instrument.

NOTE:

Before System Self Test is performed, the 6-Position Peltier Controlled Cell Changer should be installed. Otherwise, the instrument can be damaged electrically and do not operate properly.

Start the System Self Test after warming up the system for at least 20 minutes.

3. Double-click on the UV Express folder and select Thermal Denaturation mode.

4. Select OK after finishing the System Self Test.

5. Select New to open a new window and select OK.

When the 6-Position Peltier Controlled Cell Changer is connected for the first time, you need to correct the cell position. Calibrate the beam position of the 6-Position Peltier Controlled Cell Changer referring to the chapter Calibration of Multi-Cell Position (p30).

6. Open the method in main menu or mode icon on the left side of the main window. Set parameters for Experiment.

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a. Y Unit: Select one of the display unit: Absorbance, Transmittance, Reflectance or Energy. b. SBW (nm): Select bandwidth. There are five bandwidths selectable: 0.5, 1, 2, 5 or 20 nm. c. Tm Method: Select a method for determining melting temperature. Options include: 1st

derivative and Average.

d. Equation Name:

Enter the name of the formula. %G-C is entered as default, of which equation is formulated in Equation Expression.

e. Equation Expression

The default equation for the calculation of %G-C base pairs is:

%G-C=2.44 * (Tm - 81.5 - 16.66 * log(M) + 500/K)

Where M is the molarities of salt in mol/L, K is the DNA base pair length. %G-C will be calculated with estimated T

and input values, Molarities (M) and length of DNA (K).

f. DNA Pair Length (K)

Enter the DNA base pair length. This value will be used calculating %G-C.

g. Calculation Start Temperature (°C)

Enter the start temperature for calculating the T

value.

h. Calculation End Temperature (°C)

Enter the end temperature for calculating the T

value.

i. Molarity (mol/L)

Enter the salt molarities of the solution [mol/L]. This value will be used in calculating %G-C.

7. After setting parameters for Experiment and Lamp, click the Accessory tab. And select Multi-

Cell Peltier.

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8. Select Connection Check, then Method Setup will be activated. Select Method Setup.

NOTE:

Check the communication between Computer and Peltier Controller referring to the chapter Setting USB to RS-232 Cable (p15) if the Method Setup is not activated.

9. The Peltier window will be shown. Select the Multi Cell Peltier tab and set parameters according

to the experiment conditions.

▶Multi-Cell holder

a. Available Cells

Indicate cells that are available for measurement. In the case of 6-Position Peltier Controlled Cell Changer, six cells are available.

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b. Using Cells

Show the cell positions which are selected for measurement. Remove the cells by and

switch positions by and keys.

c. Multi Zero

If checked, zero will be measured all the selected positions. If not, zero will be only measured at

cell position among the using cells.

the 1

NOTE:

To prevent inadequate baseline (zero) measurement because of the difference in transmittance among cuvettes or any other reasons, Multi Baseline (Multi Zero) measurement is recommended.

d. Temperature (°C): Enter the returning temperature after the experiment is finished. e. Monitor

Select temperature that used to monitor during measurement. Options include: Block, Probe1 or

Probe2. f. Start (°C): Enter the start temperature for the measurement. g. End (°C): Enter the end temperature for the measurement. h. Interval (°C)

Enter the measurement interval temperature. For instance, if you enter 5°C, the sample will be

measured from start temperature to end temperature every 5°C. i. Rate (°C/min): Enter the heating (or cooling) temperature rate in each temperature range.

The rate has to be set under or equal to interval value.

j. Hold (min)

Enter the holding time. For example, if you set 1 min, measurement will start one

minute passed after temperature reaches at the set point. k. Error Range(°C/min)

It shows the temperature tolerance between the sampling and monitored temperature. The measurement will start when the temperature tolerance reaches within the set Error Range. Example: Set the starting temperature at 20°C and error range with 1°C then, the measurement will start when the temperature reaches between 19°C and 21°C.

▶Temperature Display

l. Select which temperature will be displayed on the panel:Block, Probe 1 or Probe 2.

The selected temperature is only displayed in real time. m. Offset:

This function is used to adjust temperatur e. It is only used for manufacturing process, so

do not modify the values.

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10. After completing the parameter setup, select Apply and OK. Then the LED for ON-LINE is turned on, and it will start heating up or cooling down to the Start temperature in the Multicell Peltier tab.

NOTE:

11. Insert blank solutions into the both reference and sample cell holder. Select Baseline and then baseline will be measured when it reaches the set Start Temperature.

To monitor the probe temperature, the probes should be immersed in the sample, or to use the block

temperature for monitoring, the cell lid should be closed tightly.

a. Start Temp (°C): The starting temperature of experiment. b. SP Temp (°C): The set temperature to go to the next measurement temp. c. Block Temp (°C): The temperature of Cell Block. d. Probe 1 Temp (°C): The temperature of Probe 1. e. Probe 2 Temp (°C): The temperature of Probe 2. f. Monitor: Selected temperature is being monitored.

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g. Error Range (°C): It shows the temperature tolerance between the sampling and monitored

temperature. The measurement will start when the temperature tolerance reaches within the set

Error Range. h. Hold Time: It shows the set holding time. i. Elapsed time (s): It shows the elapsed time to reach the sampling temperature. j. Status: It shows the status of experimental progress.

12. Remove blank solution from sample cell holder and insert sample solution into the sample cell holder. Select Sample, then the measurement will be started after the temperature reaches the target temperature.

NOTE:

To pull out the cell easily, use the cell lifting knob.

Figure 23 Pull out the cell

13. After the measurement is finished, the results are displayed in the result window. Save or print the results as required.

Other Measurement Modes

Peltier Temperature Control Unit is available in all methods, however, unlike Thermal Denaturation, the

measurement is only performed with isothermal state

NOTE:

1. Install the 6-Position Peliter Controlled Cell Changer referring to the chapter Installation.

2. Close the sample compartment cover and turn on the instrument.

When executing System Self Test, the 6-Position Peltier Controlled Cell Changer has to be installed. Otherwise, the instrument can be damaged electrically and does not operate properly.

3. Double-click on the UV Express folder and select one of the modes except Thermal Denaturation. In this case, open kinetics mode as example.

4. Select OK after finishing the System Self Test.

5. Select New to open a new window and select OK.

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NOTE:

6. Open the method in main menu or click the mode icon on the left side of the main window. Set parameters for Experiment.

NOTE:

If you want to know about the meaning of each parameter of method tabs and other modes more detail, please refer to X. Measurement Modes of the UV Express Software User Guide.

7. After setting parameters for Experiment, Lamp and Kinetics, click Accessory tab. Select Multi-Cell

Peltier.

8. Select Connection check, the Method Setup will be activated. Select Method Setup.

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NOTE:

Check the communication between Computer and Peltier Controller referring to the chapter Setting USB to RS-232 Cable (p15) if the Method Setup is not activated.

9. The Peltier window will be shown. Select the Multi Cell Peltier tab and set parameters according to the experiment conditions.

▶Multi-Cell holder

a. Available Cells

Indicate cells that are available for measurement. In the case of 6-Position Peltier Controlled Cell Changer, six cells are available.

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b. Using Cells

Show the cell positions which are selected for measurement. Remove the cells by and switch positions by and keys.

c. Multi Zero, Multi Baseline

If checked, zero (baseline) will be measured all the selected positions. If not, zero (baseline) will

be only measured at the 1

cell position among the using cells. Multi Baseline is only available at

the Scan and Scanning Kinetics mode.

NOTE:

To prevent inadequate baseline (zero) measurement because of the difference in transmittance among cuvettes or any other reasons, Multi Baseline (Multi Zero) measurement is recommended.

d. Temperature (°C): Enter the returning temperature for the experiment. e. Monitor

Select temperature that used to monitor during measurement. Options include: Block, Probe1 or Probe2.

f. Error Range (°C/min)

It shows the temperature tolerance between the sampling and monitored temperature. The measurement will start when the temperature tolerance reaches within the set Error Range.

Example: Set the starting temperature at 20°C and error range with 1°C then, the measurement will start when the temperature is reached between 19°C and 21°C. ▶ Temperature Display

g. Select which temperature will be displayed on the panel:Block, Probe 1 or Probe 2. The only

selected temprature is displayed in real time.

h. Offset:

This function is used to adjust temperatur e. It is only used for manfacturing process, so

do not modify the values.

10. After completing the parameter setup, select Apply and OK. Then the LED of ON-LINE is turned on, it will start heating up or cooling down to the set temperature in the Setup Multi Cell Peltier tab.

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11. Insert blank solutions into the both reference and sample cell holder. Select Baseline (or Zero) and

NOTE:

then baseline (or zero) will be measured when it reaches the set Temperature.

To monitor the probe temperature, the probes should be immersed in the sample, or to use the block

temperature for monitoring, the cell lid should be closed tightly.

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It shows the temperature tolerance between the sampling and monitored temperature. The measurement will start when the temperature tolerance reaches within the set Error

Range. h. Hold Time: It shows the set holding time. i. Elapsed Time (s): It shows the elapsed time to reach the sampling temperature. j. Status: It shows the status of experimental progress.

NOTE:

To pull out the cell easily, use the cell lifting knob.

Figure 24 Pull out the cell

13. After the measurement is finished, the results are displayed in the result window. Save or print results as required.

Calibration of Multi-Cell Position

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NOTE:

Calibrate the beam position of the 6-Position Peltier Controlled Cell Changer when the Multi-Cell is installed for the first time or beam position is incorrect.

1. Execute one measurement mode of UV Express software (Scan, for example) and open the Method

Setup of the Multi-Cell Peltier.

①

②

③

2. Select the Calibration tab in the Peltier setup. The following dialog box will appear.

3. The Functions of the Multi-Cell Calibration are shown as follows.

Command Function

MultiCell Position It shows saved steps of each cell position of the Multi-Cell.

It is used for moving Multi-Cell position as clicking buttons

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NOTE:

Multicell Move

It is used for moving Multi-Cell position using buttons

by entered step. Multicell Calibration Perform the Multi-Cell calibration. Stop Stop operation during Multi-Cell calibration. Save Result It is used to save the calibrated result. Reset Multicell Move to ‘0’ step of Multi-Cell position.

Although eight positions (steps) are displayed, only the first six (6) positions are used for 6-Position Peltier Controlled Cell Changer. The last two positions remain 0 steps.

4. Click Reset Multicell to format the Multi-Cell steps. Click OK.

5. Click MultiCell Calibration. Then the following dialog box will appear. Remove all samples from the 6-Position Peltier Controlled Cell Changer. [Empty the 6-Position Peltier Controlled Cell Changer]. Click OK.

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6. Then the Multi-Cell calibration will start. The current process of calibration will be shown in the main window.

7. When calibration is finished, the following box appears. Click OK.

8. Click Save Result to save the result. If the following message box appears, click OK.

Peltier Controller Auto Tuning Setup

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NOTE:

Peltier controller can be compatible with various peltier cell holders (Single or Multi). Whenever you exchange the existing peltier cell holder to another one, you should perform the “AUTO TUNING” set up to minimize the temperature fluctuation at the target temperature.

1. Push the Manual button on the control panel of the peltier controller, and then the button light is on.

2. Set any SP temperature between 30~37°C using the button.

3. Press and hold the SET/ENT button until “G.AT” is shown.

4. Push the SET/ENT button again.

5. Push the button (OFF ON).

6. Press the SET/ENT button, and then the MAN LED will blink.

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7. The MAN LED will be off after a few minutes.

8. Push the Manual Button (Stop) and then the light is off.

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Troubleshooting

Power LED is not lit on

1. Check the connection of the power cord or the fuse. The fuse is located at the rear of the instrument.

There is risk of receiving a fatal electric shock if the fuses are replaced with the power cord connected.

WARNING

2. Turn off and unplug the instrument.

3. Locate the fuse cover on the left rear panel of the instrument.

4. Carefully open the compartment latch where the fuse is located.

Il y a un risque d’électrocution si les fusibles sont remplacés tandis que le cordon d'alimentation est encore branché.

Location of latch on the fuse compartment door

5. Disconnect the fuse.

6. Replace with a new T5AL fuse (AC 250V). One spare is contained in the power module.

7. Close the compartment door.

8. Plug in the instrument and turn on.

On-Line LED is not lit on

1. Check whether the communication cable is connected tightly.

2. Check whether the Air Vent Manual button is switched off.

3. Check whether the emergency switch is off.

4. When you use the USB to RS-232 cable, change the port setting referring to the chapter

USB to RS-232 Cable (p15).

Setting

FAN LED is lit on with an alarm sound

1. Fan needs to be replaced.

Low Water Level’ LED blinks with an alarm sound

1. Check the coolant level and if it is lower than L (low) mark, add more coolant.

2. Restart the Peltier Controller.

Flow Sensor LED is lit on with an alarm sound

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1. Check whether the tubing is bent or whether it is connected correctly, and then restart the Peltier Controller.

2. Push the Air Vent Manual button on and check if coolant flows properly for about one minute after the error occurs. If the Flow Sensor LED is continuously on with the alarm sounding, contact your PerkinElmer service representative.

Pump LED is lit on with an alarm sound

1. Check whether the tubing is bent or whether it is connected correctly, and then restart the Peltier Controller.

2. Push the Air Vent Manual button to be switched on and check if coolant flows properly for about one minute after the error occurs. If the Pump LED is continuously on with the alarm sounding, contact your PerkinElmer Service representative.

Connection is failed

1. Check the Interface connector is lined properly.

2. Check whether the MANUAL RUN/STOP button is pushed on. If yes, turn off the Manual button.

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6-Position Peltier Controlled Cell Changer does not move

1. Check if the 6-Position Peltier Controlled Cell Changerconnector is connected to the Lambda 365.

2. Turn on and off again the Lambda 365 with installed 6-Position Peltier Contr olled Cell Changer.

Intensity is too low

1. Recalibrate if the light beam does not reach the center of the cell holder’s hole.

2. If the intensity value is still low after the recalibration, the lamps of the light source may be deteriorated and need replacing. Contact Perkin Elmer or the nearest representative.

When 6-Position Peltier Controlled Cell Changer does not reset

1. If reset is failed, the photo interrupt switch inside the 6-Position Peltier Controlled Cell Changerneeds replacing. Contact Perkin Elmer or the nearest representative.

When 6-Position Peltier Controlled Cell Changer is not aligned precisely

1. Recalibrate the 6-Position Peliter Controlled Cell Changer if the light beam does not reach the center of the cell holder’s hole.

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PerkinElmer Lambda 365 Installation Instructions Manual

Specifications and Main Features

Frequently Asked Questions

User Manual