OLYMPUS SCAN User Manual [ru]

®

INSTRUCTIONS

AUTOMATED IMAGE ACQUISITION

HARDWARE

This instruction manual describes the hardware components controlled by the
Olympus
optimum performance and familiarize yourself fully with the use of these products, we
recommend that you study this manual thoroughly before operation. Together with
this manual, please also read the
manual as well as the manuals of all other devices controlled by this software in order
to understand general operation methods. Retain this manual in an easily accessible
place near a system for future reference.

Automated Image Acquisition Software. To ensure safety, obtain

Automated Image Acquisition Software

OLYMPUS SOFT IMAGING SOLUTIONS GMBH

Rupert-Mayer-Strasse 44
D-81379 München
Tel: +49 89 - 89 55 805 660
Fax +49 89 - 89 55 805 6606
Email: [e-mail]

www.olympus-sis.com

Imaging Excellence

We at Olympus Soft Imaging Solutions GmbH have tried to make the information in this manual as
accurate and reliable as possible. Nevertheless, Olympus Soft Imaging Solutions GmbH disclaims any
warranty of any kind, whether expressed or implied, as to any matter whatsoever relating to this man­ual, including without limitation the merchantability or fitness for any particular purpose. Olympus Soft
Imaging Solutions GmbH will from time to time revise the product described in this manual and re­serves the right to make such changes without obligation to notify the purchaser. In no event shall
Olympus Soft Imaging Solutions GmbH be liable for any indirect, special, incidental, or consequential
damages arising out of purchase or use of this manual or the information contained therein.

No part of this document may be reproduced or transmitted in any form or by any means, electronic or
mechanical, for any purpose, without the prior permission of Olympus Soft Imaging Solutions GmbH.

© 2006 – 2008 by Olympus Soft Imaging Solutions GmbH. All rights reserved.

Manual version: 2.1, December 2008

XDeclaration of Conformity

Robert-Koch-Strasse 9
D-82152 Planegg

declares under its own responsibility that the following products

Imaging Station for Life Science Applications *

to which this declaration refers are in conformity with the

Low Voltage Directive 73/23/EEC

and the

EMC Directives 89/336/EEC and 93/68/EEC

and the following standards:

EN 61010-1: 2001 (Low Voltage)

EN 61326:1997 + A1: 1998 + A2: 2001 (EMC).

Michael Czempiel and Dr. Matthias Seel, Managing Directors

October 08, 2005

* The hardware of the basic scan^R system is identical to that of the basic cell^R system.

Hardware Manual Contents

Contents

1 Introduction and Overview ................................................................................1

1.1 Abstract ........................................................................................................2

1.2 Technical Support......................................................................................... 3

1.3 Symbols ........................................................................................................3

1.4 Checklist: Standard scan^R Components ...................................................4

2 Illumination System MT20..................................................................................5

2.1 Safety Precautions and Handling Instructions..............................................6

2.1.1 MT20 Safety Precautions .............................................................................6

2.1.2 Special Safety Precautions Regarding the Arc Burners ............................... 8

2.1.3 Handling Instructions and Recommendations .............................................9

2.1.4 Safety Labels ..............................................................................................10

2.2 Illumination System MT20 Overview ..........................................................11

2.2.1 The Front Panel...........................................................................................11

2.2.2 Top: the Burner Flap ...................................................................................12

2.2.3 Right Side: the Filter Wheel Flap ................................................................12

2.2.4 The Back Panel...........................................................................................13

2.2.5 Operation .................................................................................................... 13

2.2.6 Specifications and Technical Data of the MT20 .........................................14

2.2.7 Fuse Exchange ...........................................................................................15

2.2.8 Specifications / Technical Data of the Real-Time Controller Connectors .. 15

3 Epi-Fluorescence Illuminator ..........................................................................17

3.1 Features ......................................................................................................18

3.1.1 Light Guide Port.......................................................................................... 18

3.1.2 Adjustment Screws for Centering the Field of Illumination.........................19

3.1.3 Filter Slider for Dispersion Filter and Exciter Balancers .............................19

3.1.4 Light Sensor................................................................................................ 19

3.1.5 Flange for the IX81 Frame...........................................................................19

4 System Assembly and Adjustment .................................................................21

4.1 Overview: Connectivity of System Components ........................................ 22

4.2 Connecting the Modules of the scan^R Screening Station .......................23

4.2.1 Connecting the MT20 .................................................................................23

4.2.2 Mounting and Connecting the Epi-Fluorescence Illuminator .....................24

4.2.3 Connecting the Camera.............................................................................. 25

4.2.4 Connecting the Imaging PC to the Modules ..............................................25

4.2.5 Configuring the Imaging PC Network Connections.................................... 26

4.3 Preparing the Illumination System MT20.................................................... 27

4.3.1 Burner Installation and Exchange...............................................................27

4.3.2 Burner Alignment ........................................................................................ 28

4.3.3 Filter Exchange ........................................................................................... 31

4.4 Centering the Illumination ...........................................................................33

4.5 Optimizing the Illuminator Tube Position....................................................34

4.6 Connecting the Controller of the Motorized Stage.....................................36

4.7 PC-to-Controller Network Connection .......................................................37

Contents Hardware Manual Chapter 1 – Introduction and Overview 1

1 Introduction and Overview

Thank you very much for purchasing Olympus' state of art Screening Station for Life Science
and for your confidence in our products and services. It is Olympus main objective to provide you with
solutions able to meet your experimental demands and thus pave the way to your scientific success.

The hardware described here constitutes essential parts of the Olympus Screening Station
hardware components are intended to be controlled via the
image acquisition of biomedical samples. In combination with the
hardware is intended for the use in biomedical research.

Acquisition Software for automated

Acquisition Software the

. The

2 Chapter 1 – Introduction and Overview

The

Analysis Software, the Acquisition Software as well as the hardware

components of the Olympus Screening Station for Life Sciences are for research
use only.

1.1 Abstract

The Screening Station for Life Science scan^R is based on the hardware of the cell^R Imaging Station
and consists of the following hardware components:

— Illumination System MT20

— Illumination Coupling to the microscope (epi-fluorescence illuminator with light fiber)

— Real-time Controller board inside PC

— CCD camera

You will find a detailed description of these components, handling and alignment instructions as well as
advice how to maintain the performance of your system on the following pages. Keep in mind that all
the components are designed to work together as an integrated system. Taking away or replacing sin­gle components without prior consultation of Olympus Soft Imaging Solutions is strongly discouraged
and will most probably impair the performance of the system dramatically. Any damages to the system
due to such mishandling will not be covered by the guarantee.

All necessary tools for handling and alignment are shipped with the system.

The Imaging Station scan^R is compatible with the Olympus microscope IX81.

An additional installation of a standard Olympus epi-fluorescence Xe, Xe/Hg or Hg lamp housing e.g.,
U-LH100HGAPO, U-LH75XEAPO) is not possible because the microscope's fluorescence excitation
port is occupied by the OBS epi-fluorescence illuminator.

This instructions manual is for the hardware components of the Olympus Soft Imaging Solutions
Screening Station scan^R. To ensure safety and optimum performance as well as to familiarize yourself
with the use of the system we recommend you to study this manual thoroughly before operating the
system. Keep this manual in an easily accessible place near the Imaging Station for future reference.

The scan^R system meets the CE, UL and FCC requirements.

nd

IEC/EN 61010-1(2

ed.), UL61010-1,

EN61326 Class A, FCC Part 15 Class A (Illumination System MT20: class A and B).

Hardware Manual Chapter 1 – Introduction and Overview 3

1.2 Technical Support

If you find any information missing in this manual or you need additional support, please contact your
local Olympus representative.

1.3 Symbols

The following symbols are placed on the Illumination System MT20. Study the meaning of the symbols
and always use the equipment in the safest possible manner.

General symbols

Symbol Explanation

Carefully read the instructions on the label and the manual before use. Improper use
could result in personal injury to the user and/or damage the equipment.

The surface becomes hot and should not be touched with bare hands

Parts may be under high voltage and should not be touched.

The main switch is turned OFF.

The main switch is turned ON.

This symbol indicates separate collection of waste electrical and electronic equipment in

the EU countries.

Do not throw the equipment into the domestic refuse

Please use the return and collection systems available in your country for the disposal of

this product

4 Chapter 1 – Introduction and Overview

1.4 Checklist: Standard scan^R Components

Before assembly, please check whether you have the complete set of standard scan^R system com­ponents as listed below.

a Microscope IX81 with control unit IX2-UCB

b Illumination System MT20 consisting of:

— MT20 housing with integrated lamp house, filter wheel, shutter and attenuator

— short arc burner

— power cord

— Illumination System connector cord (blue RJ45 cable)

— Light sensor connector cord (see photo in Chapter 7, System Assembly and Adjustment)

— optical fiber (quartz light guide; see photo in Chapter 7, System Assembly and Adjustment)

— set of hex-wrenches

— cotton gloves

c Fluorescence filter set consisting of:

— Dichroic mirror and emission filter mounted in Olympus filter cube

— Excitation filter(s) to be mounted in the MT20 filter wheel

d MT20 epi-fluorescence illuminator to couple the illumination system to the microscope via the opti-

cal fiber

e CCD camera

— FireWire

— trigger cable

TM

(IEEE 1394) cable

f Motorized stage with controller

g cell^R imaging computer including

— cell^R Real-time Controller board

— FireWire

— Front panel with three digital I/O ports

— scan^R acquisition and analysis software installed

— computer monitor with cable

— 2 power cords

— software protection key (dongle)

TM

(IEEE 1394) card

h scan^R acquisition and analysis software on CD-ROM

i Manuals

j Z-drift control nit ZDC mounted on the IX81 frame (optional)

k Climate chamber (optional)

Hardware Manual Chapter 2 – Illumination System MT20 5

2 Illumination System MT20

This chapter refers only to the fluorescence Illumination System MT20. Before using this unit together
with the other system components, the microscope and associated devices, make sure that you have
carefully read and understood their respective manuals, and understand how the entire system should
be operated.

2.1 Safety Precautions and Handling Instructions..............................................6

2.1.1 MT20 Safety Precautions .............................................................................6

2.1.2 Special Safety Precautions Regarding the Arc Burners ............................... 8

2.1.3 Handling Instructions and Recommendations .............................................9

2.1.4 Safety Labels ..............................................................................................10

2.2 Illumination System MT20 Overview ..........................................................11

2.2.1 The Front Panel...........................................................................................11

2.2.2 Top: the Burner Flap ...................................................................................12

2.2.3 Right Side: the Filter Wheel Flap ................................................................12

2.2.4 The Back Panel...........................................................................................13

2.2.5 Operation .................................................................................................... 13

2.2.6 Specifications and Technical Data of the MT20 .........................................14

2.2.7 Fuse Exchange ...........................................................................................15

2.2.8 Specifications / Technical Data of the Real-Time Controller Connectors .. 15

6 Chapter 2 – Illumination System MT20

2.1 Safety Precautions and Handling Instructions

For Laboratory Use Only.

Danger: Ultraviolet radiation is emitted from this product. Avoid exposure. ALWAYS WARE

PROTECTIVE CLOTHING. EXPOSURE MAY CAUSE PREMATURE AGING OF THE SKIN
AND CANCER. ALWAYS WARE PROTECTIVE EYEWEAR; FAILURE TO DO SO MAY
RESULT IN SEVERE BURNS OR LONG TERM INJURE TO THE EYE. Never look directly
into the lamp. Exposure can cause eye and skin allergy and allergic reactions. Medications
or cosmetics may increase your sensitivity to ultraviolet radiation. Consult a physician be­fore operating this product if you are using medications or have a history of skin problems
or believe yourself particularly sensitive to sunlight.

Always have the microscope's UV cut plate mounted as protection against UV radiation.

Color fading of specimens: This system features high excitation light intensity to ensure
bright observation of dim fluorescent specimens. Consequently, after longer periods of ob­servations using high-power objectives, the colors of specimens will fade quicker than
usual, causing the intensity and contrast of fluorescent images to deteriorate. In such cases
it is advisable to reduce excitation light intensity to slow down color fading. To reduce the
light intensity use the built-in attenuator as explained in this manual.

Engage the shutter of MT20 as explained in this manual (or use the shutter of the micro­scope) even if you interrupt observation only for a short time.

2.1.1 MT20 Safety Precautions

a Always use a power cord provided or approved by Olympus Soft Imaging Solutions.

b Provide unimpaired access to the main power switch at the rear panel.

c No electrical cord should be connected or disconnected while the power switch is set on (“I”). Make

sure that the unit's main power switch is set off (“O”) before plugging or unplugging the power cord
to the power outlet.

d Always make sure that the grounding terminal of the Illumination System MT20 and the wall outlet

are connected properly. If the System is not grounded, Olympus Soft Imaging Solutions cannot war­rant the electrical safety and performance of the system.

e When installing the Illumination System MT20 leave at least 100 mm free air space at the rear panel

and at least 50 mm at the sides and on top to allow air circulation. The Illumination System MT20
has an internal fan to cool the arc burner. The air outlet is located at the rear of the housing while
the inlet grid is at the bottom.

Hardware Manual Chapter 2 – Illumination System MT20 7

f The Illumination System MT20 must be placed on an even, stable and flat surface and standing on

its supports. Make sure that no cloth or paper can clog the air inlet slits at the bottom of the hous­ing.

g The arc burner has to be exclusively one of the two types specified by Olympus Soft Imaging Solu-

tions. No other arc burner may be used with the Illumination System MT20.

h Do not open the burner flap of the Illumination System MT20 while the arc burner is turned on. First

turn off the arc burner by software and wait for at least 30 minutes while the cooling fan is operat­ing. Set the main power switch on “O” (OFF) before opening the burner flap. The arc burner socket
and all parts in the vicinity of the burner will be extremely hot during operation and will cause ther­mal injury if touched.

i Never attempt to disassemble the Illumination System MT20 or open its housing. The internal power

supply units and electrical circuits contain high voltage components and can cause severe electrical
shock and thus are not serviceable by the user. Warranty will be lost if the housing is opened.

j Do not operate the Illumination System MT20 under environmental conditions other than specified

in this manual (see Chapter 2.2.6., Specifications and Technical Data).

k Do not look directly into the light emitted by the arc burner with unprotected eyes under any cir-

cumstances. The emitted light is very intense and has a very high UV-content which may cause irre­versible damage to your eyes.

l Avoid direct exposure of unprotected skin to the light emitted by the arc burner. It can cause severe

burns.

m Direct exposure to the arc burner light may occur at the following positions:

— Illumination System’s housing at the fiber port if the fiber is removed

— Exit of the optical fiber

— Exit of the illumination coupling (epi-fluorescence illuminator)

— Beam path of the microscope (which upon proper usage is not accessible by the user)

— Exit of the objective (or at the nose piece, if no objective is mounted)

n If the equipment is used not as specified by this manual, the safety and performance of the equip-

ment may be impaired. In addition, the equipment may also be damaged and warranty may be lost.
Always use the equipment as outlined in this instruction manual.

8 Chapter 2 – Illumination System MT20

2.1.2 Special Safety Precautions Regarding the Arc Burners

a Handling the arc burner bulb is potentially dangerous. Wear a protective mask, leather gloves and a

thick long-sleeved shirt or coat during handling and disposal (see below).

b The arc burner bulb is under high internal pressure. Do not hit the arc burner bulb against anything

nor scratch it or apply excessive stress. Avoid vibrations. Otherwise the arc burner bulb could ex­plode and cause injuries.

c For transport and storage always place the arc burner in the provided protective case.

d Take care to avoid leaving fingerprints or dirt on any part of the high pressure arc burners. If con-

taminated, clean by wiping gently with gauze. To remove fingerprints or oil stains, wipe with gauze
slightly moistened with lens cleansing fluid.
Since these fluids are highly inflammable, be careful to keep them away from open fire, heat or hot
surfaces and potential sources of electrical sparks, such as main switches.

e The arc burners have an average life time as specified in this manual (see Chapter 2.2.6, Specifica-

tions and Technical Data). When the arc burner hour counter (see Chapter 4.3.2, Burner Alignment)

gives a reading higher than the specified life time, replace the burner with a new one following the
instructions for replacement (see Chapter 4.3.1, Burner Installation and Exchange) and disposal of
used burners (see below). Arc burners that are used longer than the rated service life time will have
a reduced intensity and stability, are likely to fail and might explode because of the deteriorated
glass condition.

f Arc burners not in service must always be kept in the provided protective case.

g Hg/Xe burner bulbs contain poisonous mercury. They have to be treated with special care. If they

explode during service mercury gas might be dispersed in the environment.

h Hg/Xe burners must be treated as hazardous waste. Always follow national and local laws and

guidelines when disposing of Hg/Xe burners. It is recommended to commission a professional
waste disposal company with the disposal. Hand over the burner in its original packaging.

i Xe burners: It is recommended to commission a professional waste disposal company with the

disposal. Hand over the burner in its original packaging. Disposal without breaking the glass part
may result in burner bulb explosion. Follow the instructions below to prevent harmful repercussions.

— Ware protective clothing.

— Wrap the used lamp in a thick cloth.

— Break the glass part of the lamp into pieces using a hammer.

— Dispose of as industrial waste (except Hg/Xe burners, see above).

Hardware Manual Chapter 2 – Illumination System MT20 9

2.1.3 Handling Instructions and Recommendations

a The Illumination System MT20 is a precision instrument. Handle it with great care and avoid subject-

ing it to sudden or severe impact. Also connect each cable gently.

b The built-in ventilating fan and the built-in filter wheels may generate vibrations and sound. There-

fore it is recommended not to place the Illumination System MT20 on the same table as the micro­scope.

c With exception of the burner flap and the filter flap the Illumination System MT20 may only be

opened and disassembled by authorized service personal. Otherwise warranty is lost.

d Before using the Illumination System MT20 for the first time check that the burner is installed cor-

rectly and that all cords are connected correctly.

e Before opening the burner flap for replacement of the burner, turn it off by software (OBS System

Configuration) and wait for at least 30 minutes while the cooling fan is operating. Then set the main
power switch on “O” (OFF) and open the burner flap. (Do not open the burner flap of the Illumination
System MT20 while the arc burner is turned on.)

f Do not place the unit inside a climate chamber.

g Do not bend the optical fiber to a diameter of less than 45 cm. The optical fiber is fragile and may

brake if bent too strongly or if torsional stress is applied.

h Cover the ends of the optical fiber with the protection cover if they are not attached to the Illumina-

tion System MT20 or to the microscope.

i Always operate the Illumination System MT20 in an upright position. Never lay it on the side during

operation. This could cause the lamp to burst, damage the equipment and cause injury.

j Before transport of the system remove the lamp and store it safely in the provided protective case.

k Mind that turning the arc burner on and off repeatedly will shorten the life span.

l To clean the surface of the Illumination System MT20, use a lint-free, dry and soft cloth. Never at-

tempt to use organic solvents or strong detergents.

m Take care to avoid leaving fingerprints or dirt on the optical filters, the lenses at the ends of the light

guide or the optics of the illumination coupling. If contaminated, clean by wiping gently with special
optical cleansing tissue. To remove fingerprints or oil stains, wipe with special optical cleansing tis­sue slightly moistened with lens cleansing fluid. Use the cotton gloves when handling light filters.
* Since these fluids are highly inflammable, be careful to keep them away from open fire, heat or hot
surfaces and potential sources of electrical sparks, such as main switches.

10 Chapter 2 – Illumination System MT20

2.1.4 Safety Labels

The following symbols are found on the Illumination System MT20. Study the meaning of the symbols
and always use the equipment in the safest possible manner.

Lamp housing safety label

Label Note Location

Carefully read
the instructions
on the label and
the manual
before use.
Improper use
could result in
personal injury
to the user
and/or damage
the equipment.

Top side of
the Illumina­tion System
MT20 / MT10

Fiber port safety label

Label Note Location

Carefully read the instructions
on the label and the manual
before use. Improper use
could result in personal injury
to the user and/or damage the
equipment.

Front side of the
Illumination System
MT20 / MT10

Hardware Manual Chapter 2 – Illumination System MT20 11

K

r

T

2.2 Illumination System MT20 Overview

2.2.1 The Front Panel

BURNER

ADJUSTMEN

BURNER ON

CONTROLLER

connecto

LOC

LED:

FIBER lock

Fiber
port

Light sensor
connector

LED:

Status

Filter wheel
connector

LOCK: 2 mm hex-wrench opening to lock or release the arc burner for installation and replacement.

BURNER ADJUSTMENT: three handles to align the burner.

BURNER ON LED: On / off Æ arc burner on / off

CONTROLLER connector: RJ45 plug to connect the Illumination System MT20 to the cell^R Real-
Time Controller.

FIBER LOCK: 1.5 mm hex-wrench opening to lock or release the optical fiber in the fiber port.

Fiber port: Opening for the optical fiber.

LIGHT SENSOR connector: 3-pin connector for light sensor cable.

Filter wheel connector: 15-pin sub-D port, currently without use.

STATUS LED: Off Æ no connection to cell^R Real-Time Controller.

On Æ active connection to cell^R Real-Time Controller, ready for operation.
Regular flashing Æ Illumination System MT20 in stand-by mode.
Irregular activity Æ Illumination System MT20 in action.

12 Chapter 2 – Illumination System MT20

A

2.2.2 Top: the Burner Flap

Opening for a 3 mm
hex-wrench

ccess to the arc

burner for replacement

Before opening: Wait until system has cooled down, then switch main power off!

2.2.3 Right Side: the Filter Wheel Flap

The flap has to be closed and the subsequent automatic initialization has to be completed
for operation.

Hardware Manual Chapter 2 – Illumination System MT20 13

A

2.2.4 The Back Panel

rc burner cooling

air outlet

Main power switch, fuse-holder
and AC power input connector.

Do not block ventilation slits! Keep 10 cm distance from wall.

2.2.5 Operation

Only operate the system when completely installed and connected to a microscope.

For operating set main power switch on “I” (ON). By doing so the System will execute a routine to ini­tialize and test the electro-mechanical components and motors.

For further operation run the scan^R software or the OBS System Configuration on your computer
according to the software manual.

14 Chapter 2 – Illumination System MT20

2.2.6 Specifications and Technical Data of the MT20

Dimensions and Weight: H 354 x W 236 x D 315 mm, 13 kg

Power supply rating: 100 to 120 V and 200 to 240 V AC, 50 – 60 Hz (47 – 63 Hz), 3.9 / 1.6A

Fuse: 250VAC, T4AH (5 x 20mm)

Enclosure rating: IP20

Short arc burner:

— Type 1: 150 W Xe, high pressure arc burner, Olympus _MT_ARC/XE, article No. E0435001, ex-

pected minimal lifetime under standard laboratory conditions: 1000 h

— Type 2: 150 W Hg/Xe, mixed gas arc burner, Olympus _MT_ARC/HG, article No. E0435002, ex-

pected minimal lifetime under standard laboratory conditions: 1000 h

Light output range, MT20: between 320 and 720 nm; MT20_NIR: between 380 and 800 nm

Light output power: up to 50% higher for MT20E as compared to MT20

Mechanical shutter: opening/closing time 1.0 +/- 0.2 ms

Filter wheel: 8 positions

Optical filter dimensions: ∅ 24.8 mm +/- 0.1, thickness 2 – 6 mm, maximum weight 7 g each

Filter and attenuator switching time: This is dependent on the filter load and the number of positions

to be moved.

Switching times [ms]

Load [g] < 10.5 < 21 > 21 > 28 > 35 attenuator

Next position

2nd position 86 92 102 114 129 40

3rd position 113 119 130 142 158 50

4th position 141 147 157 169 186 60 etc.

58 64 72 80 91 35

Attenuator transmission [%], 14 positions: 1, 3.1, 6.3, 10, 12.5, 25, 33.3, 37.5, 50, 62.5, 66.6, 75,

87.5, 100

Operating environmental conditions: indoor use only.

— Altitude: up to 2000 m

— Ambient temperature: 5 to 40°C

— Maximum relative humidity: 80% up to 31°C, 70% at 34°C, 60% at 37°C, 50% at 40°C

— Supply voltage fluctuation: +/- 10%

— Pollution degree 2 (in accordance with IEC664)

— Over-voltage category II (in accordance with IEC664)

LED indicators, MT20 front: BURNER ON (lower left), STATUS (lower center)

Connectors, MT20 front:

— CONTROLLER: RJ45 connection to Real-Time Controller board (PC back panel)

Hardware Manual Chapter 2 – Illumination System MT20 15

FILTER WHEEL: sub-D, 15-pin connection

—

— LIGHT SENSOR: 3 pin connection to light sensor (epi-fluorescence illuminator)

Connectors, MT20 rear:

— 3-pin AC input plug with ground

— Main switch (I: on, O: off)

Optical fiber: minimum bend diameter 45 cm short term, avoid torsional stress

— Type 1: 2 m solid quartz fiber, Olympus _MT_FIB/2m, article No. E0435007

— Type 2: 3 m solid quartz fiber, Olympus _MT_FIB/3m, article No. E0435008

2.2.7 Fuse Exchange

During standard operation the fuses will not blow. In case the fuse blows switch off the unit and con­tact your local Olympus service specialist or the Olympus Soft Imaging Solutions support team to avoid
potential hazard because the unit might be severely damaged.

The system is equipped with 2 fuses (high breaking, delayed action fuse, 250 V AC, 4 A, size: 5 x 20
mm) located below the main switch of the Illumination System MT20 at the rear of the housing.

2.2.8 Specifications and Technical Data of the Real-Time Controller Connec­tors

Connectors, cell^R Imaging Computer, front, DIGITAL I/O:

— Input: R(in) > 10 kΩ, low V < 0.8 V, high V > 2.0 V,

— Output, low level: R(out) < 90 Ω, V < 0.8 V @ I < 5.8 mA

Output, high level: R(out) < 870 Ω, V > 2.2 V @ I < 3.2 mA

If a high level voltage of 5 V is required, please contact your local support specialist.

Camera Trigger: open collector with 330 Ω pull-up to 5 V

— TTL high level: R(out) = 330 Ω, V > 2.0 V @ I < 9 mA
— TTL low level: R(out) < 90 Ω, V < 0.8 V @ I < 9 mA

PIFOC controller (analog out): SMB, 14 Bit DAC, V = 0 – 10 V (±2 mV) @ 1 MΩ R(load),
I < 1 mA, R(out) = 47 Ω @ DC, suggested R(load) > 50 KΩ

16 Chapter 2 – Illumination System MT20

Hardware Manual Chapter 3 – Epi-Fluorescence Illuminator 17

3 Epi-Fluorescence Illuminator

This chapter explains the details of the illuminator that couples the illumination system MT20 to the
IX81 microscope.

3.1 Features ......................................................................................................18

3.1.1 Light Guide Port.......................................................................................... 18

3.1.2 Adjustment Screws for Centering the Field of Illumination.........................19

3.1.3 Filter Slider for Dispersion Filter and Exciter Balancers .............................19

3.1.4 Light Sensor................................................................................................ 19

3.1.5 Flange for the IX81 Frame...........................................................................19

18 Chapter 3 – Epi-Fluorescence Illuminator

3.1 Features

The MT20 epi-fluorescence illuminator is a tube which houses the different lenses and features the fiber
port, the filter slider, the adjustments screws to center the field of illumination and the light sensor to
measure the light output during the optimization of the lamp position in the MT20. These items are
referred to in detail below.

6

9

3

2

8

1

6

4

7

5

1: illuminator tube containing the optical elements

2: light guide with fiber connector and ring to adjust depth

of penetration for focus optimization

3: light guide port

4: set screw to fix the light guide

5: light sensor with cable

6: adjustment screws for centering the field of illumination

7: filter slider with dispersion filter

8: flange for IX2 microscope frame

9: screw to fix the illuminator tube inside the flange

Optionally the illuminator may have a space-saving L-shape form. Furthermore, both a straight and a L­shape illuminator containing a field stop slider is available for the IX81 microscope.

3.1.1 Light Guide Port

The light guide features a distance ring which assures the optimum position of the fiber exit when the
fiber is moved all the way into the port. The position of the distance ring is set by Olympus Soft Imaging
Solutions and fixed by a small screw. The fiber exit is fixed inside the port by a form-fit screw. Thus, the
fiber cannot be pulled out if the fixing screw is just slightly loose.

Hardware Manual Chapter 3 – Epi-Fluorescence Illuminator 19

3.1.2 Adjustment Screws for Centering the Field of Illumination

The illumination has to be centered in order to illuminate the entire chip of the CCD camera. No tool is
needed to turn the screws. See Chapter 7.4, Centering the Illumination.

3.1.3 Filter Slider for Dispersion Filter and Exciter Balancers

The slider has four openings to hold filters of 12 mm diameter. The first position contains a dispersion
filter that allows to illuminate the entire ocular's field of view. One position should always remain free for
unaltered light throughput and illumination of the CCD chip with maximum brightness. The two other
positions are empty by default. They can be equipped with filters which are to be fixed by easily re­movable plastic rings.

3.1.4 Light Sensor

A light sensor (photodiode) is mounted inside the illuminator tube (but outside the light path) which
measures the intensity of back-scattered light. The more intense the scattered light, the higher the
overall light intensity leaving the fiber and the better correspondingly the position of the short arc
burner in the MT20 lamp house. The light sensor cable is connected to a plug in the front plate of the
MT20.

3.1.5 Flange for the IX81 Frame

The illuminators are inserted into a special flange mounted on the epi-fluorescence port of the IX81
microscope; see Chapter 4.2.2, Mounting and Connecting the Epi-Fluorescence Illuminator.

20 Chapter 3 – Epi-Fluorescence Illuminator

Hardware Manual Chapter 4 – System Assembly and Adjustment 21

4 System Assembly and Adjustment

The following chapters explain how all the different hardware modules of the scan^R Screening Station
have to be connected for correct operation.

Overview: Connectivity of System Components ........................................ 22

4.1

4.2 Connecting the Modules of the scan^R Screening Station .......................23

4.2.1 Connecting the MT20 .................................................................................23

4.2.2 Mounting and Connecting the Epi-Fluorescence Illuminator .....................24

4.2.3 Connecting the Camera.............................................................................. 25

4.2.4 Connecting the Imaging PC to the Modules ..............................................25

4.2.5 Configuring the Imaging PC Network Connections.................................... 26

4.3 Preparing the Illumination System MT20.................................................... 27

4.3.1 Burner Installation and Exchange...............................................................27

4.3.2 Burner Alignment ........................................................................................ 28

4.3.3 Filter Exchange ........................................................................................... 31

4.4 Centering the Illumination ...........................................................................33

4.5 Optimizing the Illuminator Tube Position....................................................34

4.6 Connecting the Controller of the Motorized Stage.....................................36

4.7 PC-to-Controller Network Connection .......................................................37

22 Chapter 4 – System Assembly and Adjustment

It is the user’s responsibility to provide a sufficient and stable electrical power supply

network for the scan^R system. Olympus rejects any warranty claims for any power
break down follow up damage caused by an insufficient power supply network.

4.1 Overview: Connectivity of System Components

The schematic diagram below shows the connection of standard components of the cell^R station.
Before you switch on any of the cell^R components, make sure that they are correctly connected.

1: Power cords

2: FireWire™ (IEEE 1394) cable to connect the CCD camera to the FireWire™ board of the PC for image data transfer.

3: Camera trigger line to connect the CCD camera to the computer-hosted cell^R Real-time Controller for synchronized

image acquisition.

4: Illumination System MT20 connection cable: Blue RJ45 cable to connect the MT20 to the computer-hosted cell^R Real­time Controller / cell^M System Coordinator for (synchronized) control of the sample illumination.

5: Light sensor cable to connect the light sensor in the epi-fluorescence illuminator (to monitor the optimization of the
illumination intensity) to the LIGHT SENSOR connector at the front of the MT20.

6: Optic quartz light: Blue fiber to guide the light from the MT20 to the epi-fluorescence illuminator.

Do not bend the light guide to a radius of less than 45 cm; otherwise, the quartz fiber may
become damaged.

Hardware Manual Chapter 4 – System Assembly and Adjustment 23

4.2 Connecting the Modules of the scan^R Screening
Station

4.2.1 Connecting the MT20

1. Connect the power cord to the main power connector at the MT20 rear panel and to a power

outlet. Caution: Make sure that the MT20's main power switch is set on OFF before connec­tion.

2. Connect the CONTROLLER connector at the front of the MT20 with the controller board at
the back of the PC using the blue RJ45 cable.

3. Connect the LIGHT SENSOR connector with the light sensor in the epi-fluorescence illumina­tor using the black light sensor cable (with small three-pin plugs).

4. Connect the light guide: Gently insert the thin connector of the optical fiber entirely into the
fiber port at the front panel. Keep the protective plastic caps for transport purposes.

The fiber is fragile and might break if handled without care.

— Do not bend the fiber more than to a diameter of 45 cm (short term: 35 cm).

— Do not expose the fiber to shear force or torque.

5. Lock the fiber by turning the fiber lock screw with a 1.5 mm hex wrench clockwise. Tighten
the screw gently.

24 Chapter 4 – System Assembly and Adjustment

6. For the connection of the other connector of the fiber see the following chapter.

UV radiation output; do NOT disconnect fiber while burner is switched ON.

4.2.2 Mounting and Connecting the Epi-Fluorescence Illuminator

See also the photo in Chapter 3, Epi-Fluorescence Illuminator.

2

1

1. Insert the flange with the dovetail 1 first all the way into the frame opening.

2. Fix it with the two screws in the two holes that point downward by 45° on the left and right

side of the frame using the 4 mm hex-wrench.

3. Insert the epi-fluorescence illuminator tube into the flange so that the distance between
flange and back end of the illuminator (light guide port) is as listed for the different objec­tives.

4. Fix the illuminator with the fixing screw 2 of the flange using the 2.5 mm hex-wrench.

5. Connect the female end of the light sensor cable.

6. Insert the thick end of the light guide as far as possible (until the ring to adjust the depth of

penetration makes contact with the illuminator) and fix it with the fixing screw using the 2
mm hex-wrench. The fiber cannot be pulled out if the form-fit fixing screw is just slightly
loose.

UV radiation output; do NOT disconnect fiber while burner is switched ON.

Hardware Manual Chapter 4 – System Assembly and Adjustment 25

4.2.3 Connecting the Camera

The camera has to be mounted on the microscope camera port using a c-mount adapter as described
in the microscope manual.

4.2.3.1 Camera Hamamatsu Orca AG

1. Make sure that the camera controller unit is switched off and disconnected from the power

outlet.

2. Use the 3m FireWire™ cable to connect the FireWire™ port at the back of the camera con-
troller unit to the FireWire™ card at the back of the computer.

3. Connect the trigger-out BNC port of the controller at the back of the computer to the trigger­in BNC port of the camera controller unit.

4. Connect camera and camera controller to the special multi-pin cable.

4.2.4 Connecting the Imaging PC to the Modules

1. Insert the blue RJ45 cable coming from the CONTROLLER plug on the MT20 front panel

into the central RJ45 port of the cell^R controller board.

2. Camera: Connect the BNC plug of the trigger cable to the BNC port of the cell^R controller
board.

3. Camera: Plug the FireWire™ cable coming from the camera or the camera controller (in case
of the Hamamatsu Orca AGR) into any one of the two ports of the FireWire™ card.

In addition to the interfaces at the back, the cell^R imaging computer features a DIGITAL I/O interface
on the front panel. It contains three BNC ports (1, 2 and 3) that enable to send TTL trigger pulses to
peripheral devices during an experiment.

26 Chapter 4 – System Assembly and Adjustment

USB port for

cell^R hard­ware key

2 BNC port for

camera trig­ger cable

Lemo plug to

connect with
ODB device
(U_FFWO or
IX2_FRFACA)

3 FireWire ports

Controller serial

port for motorized
stage connection

Binder plug for

ODB device
power supply

SMB port for

PIFOC control­ler cable

1 Connection

to MT20

4.2.5 Configuring the Imaging PC Network Connections

When the factory settings of the network connections to the cell^R Real-time Controller are
changed, the communication will be lost and the Imaging Station cannot be operated any­more.

It may happen that the settings of the network connections are changed, especially when the operating
system is upgraded or the imaging PC is hooked to an intranet. The following instructions allow to reset
connection settings.

1. Go to Control PanelNetwork Connections.

2. Make sure that the connection to the cell^R Real-time Controller is called CTR_R.

Hardware Manual Chapter 4 – System Assembly and Adjustment 27

3. Go to PropertiesGeneral.

4. Make sure that Internet Protocol (TCP/IP) is activated and go to Properties.

5. Use the IP address 42.42.42.17 and the Subnet mask 255.255.255.0.

6. Confirm with OK. And go to the Advanced tab.

7. Make sure that the Window FirewallSettings are off.

8. Confirm with OK.

4.3 Preparing the Illumination System MT20

4.3.1 Burner Installation and Exchange

Wear protective glasses, clothes and gloves. Avoid any kind of mechanical stress to the
burner. Allow the illumination system to cool down for at least 30 minutes with the main
power on to keep the cooling fan in the back operating. Then set the main power switch on

O (OFF). Do not touch glass parts of the burner.

1. Loosen the burner flap screw (3mm hex wrench). The flap will lift slightly. Open the flap.

2. If a burner is installed and has to be exchanged gently hold the heat sink and unscrew the

nut. Remove nut, anode cable and heat sink from the burner socket.

3. Loosen the lock screw at the front panel and take out the burner.
(Storage and/or disposal see Chapter 2.1.2, Special Safety Pre-
cautions Regarding the Arc Burners).

4. Insert the new burner with the flat end (cathode) downwards and
with the tip-off pointing in the direction indicated in the scheme
engraved on the inside of the flap (see scheme below). Be sure
not to scratch the surface of the reflector when inserting the
burner into the funnel of a MT20E.

top (anode)

tip-off

28 Chapter 4 – System Assembly and Adjustment

reflector

burner socket

anode cable

tip-off

nut

heat sink

5. Tighten the lock screw gently but firmly until the screw is blocked; force is not necessary.
The lock screw is spring loaded; you will feel the resistance of the spring once the screw is
tight.

6. Attach the heat sink, the anode cable and the nut to the screw (anode) at the top of the
burner. Hold the heat sink with the orientation as shown in the photo below while tightening
the nut.

7. Close the burner flap and gently tighten the burner flap screw. The lamp will not ignite for
safety reasons if the flap is open or just loosely closed due to an interlock mechanism.

4.3.2 Burner Alignment

The burner has to be aligned after installation for maximum performance. To do so, the light output is
constantly monitored by the light sensor in the epi-fluorescence illuminator.

ObsConfig.exe

1. Start the OBS System Configuration software by clicking the OBSConfig button and go to

the Burner page.

Hardware Manual Chapter 4 – System Assembly and Adjustment 29

2. Check if the burner type listed is correct. If not, click the Exchange burner button to open
the Select burner type… dialog box that enables you to choose the type of arc burner. Con­firm by clicking Ok.

If you install a different type of burner, that is, if you switch from a Xe to a Hg/Xe burner,
you will be asked if the one installed is used or not. If it is new, the hour counter will start
with 0 h, otherwise it will start with the last reading of this particular type of burner. Thus, if
you frequently exchange the type, you will still have control of the expired lifetime of each.

3. Start the burner by clicking on the Burner ON/OFF button and wait for about 10 min until the
light intensity stabilizes. The light sensor in the epi-fluorescence illuminator has to be con­nected to the MT20 (see Chapter 4.2.1, Connecting the MT20).

4. Move the dispersion filter mounted in the filter slider in the back of the epi-fluorescence illu­minator into the light path to increase scattered light intensity.

30 Chapter 4 – System Assembly and Adjustment

5. Open the shutter by clicking on the Shutter OPEN/CLOSE button.

6. The light output is indicated by the two blue bars. They do not represent an absolute value

but are rescaled frequently. The maximum that was reached so far is indicated by the red
lines.

The upper bar gives a coarse reading. When the maximum reaches the right end the display
is reset so that the maximum is repositioned at 80% of the length. This may happen several
times during the adjustment.
The lower bar shows an area of ±10% around the maximum of the upper bar and therefore
gives a finer reading. However, it is updated independently once its maximum reaches the
right end and thus does not necessarily show a fivefold magnification of the current display
of the upper bar. The reset may happen frequently.
In cases, upon reset no lower blue bar is visible. This may occur if immediately afterwards
the maximum is lost due to a wrong turn of one of the adjustment screws (see next step). A
click on the reset button on the right moves the lower blue bar to the center.

coarse reading

fine reading

reset button

7. Use the adjustment screws in the order left/right, up/down and back/forth. Use the first
screw and check in which direction to turn in order to increase the length of the blue bars.
Turn as long as the reading increases and then switch to the second screw and repeat the
procedure. Then switch to the third one and do the same. Usually it is sufficient to go
through this routine two or three times. Mind that the automatic resetting of the bars does
NOT indicate a worsening of the adjustment (see step above)!

left/right

back/forth

up/down

8. Display warning message. You can activate a display warning message to remind you that
the burner is still turned on if you did not switch it off before exiting the scan^R Acquisition
Software (see bottom of the OBS System Configuration screenshot at the beginning of this
chapter).

Hardware Manual Chapter 4 – System Assembly and Adjustment 31

A

4.3.3 Filter Exchange

1. Start the OBS System Configuration software as described in the chapter above and go to

the Excitation Filter page. Select the filter position that you want to exchange with the slider
bar.

2. Open the flap at the right side of the MT20. The selected filter position is now accessible and
allows the easy removal or insertion of a 25 mm filter. The filter wheel position is indicated by
labels.

The filters are held in place by metal clips. Insertion is easiest if the filter is moved into posi­tion tilted from the left side and then turned as shown in the series of drawings below. It is
thus moved under the clips and sits tightly. Likewise, the easiest way to remove a filter is to
turn it left and then pull it out. The left drawing below shows a filter moved halfway into po­sition, still tilted to the left.

Label ∇ 5 indicating
filter position Nr. 5

rrows have to point

Metal clips hold the
filters in position.

into the same direction.

Many filters are coated on one side and their performance is direction dependent. Make
sure to insert the filters so that the little arrow on its frame points into the same direction as
the arrow engraved on the right side of the filter wheel compartment.

32 Chapter 4 – System Assembly and Adjustment

3. Close the flap. The system will perform a mechanical initialization that takes about 18 sec­onds. If you listen closely you can hear the filter wheel and attenuator disk turn. After initiali­zation the STATUS LED is turned on automatically to indicate that the system is ready.

All filters provided by Olympus Soft Imaging Solutions have the standard size and fit into
the filter wheel. In case you use other filters you should check their thickness and diameter
using the templates on the inside of the filter flap.

Filters should not exceed the size given by the templates in the top row (Filters must fit in
here.) nor should they be so small that they would fit into the templates in the lower row
(Filters must NOT fit in here.). The slits on the side are for testing the thickness of the fil­ters. The same rules apply here as for the diameter.

Note that the filter wheel cannot be used via software for safety reasons while the flap is
open. It can be moved by hand but a certain mechanical resistance has to be overcome.

Note that all changes to the Illumination System MT20 (besides filter and burner exchange)
have to be done by Olympus Soft Imaging Solutions. Use optional equipment provided or
approved by Olympus Soft Imaging Solutions only. Usage of other equipment may impair
the performance or cause damage to the system. Safety of the system cannot be guaran­teed in that case. Any damage to the system resulting from such action will void the war­ranty.

Hardware Manual Chapter 4 – System Assembly and Adjustment 33

4.4 Centering the Illumination

1. Put a fluorescent sample onto the microscope stage. Most suitably the fluorescence covers

the entire field-of-view.

2. Start the scan^R acquisition software and go to Edit ScanAcquisition.

3. Move a suitable filter cube in the microscope into position.

4. Select the corresponding excitation filter of the Illumination System MT20.

5. Make sure that the dispersion filter mounted in the filter slider (see 7 in photo in next chapter)

of the illuminator is NOT in the light path.

6. Click the Live settings button.

7. Click the Ocular view button in the Live view window that opens.

8. Look through the eyepiece. You might find that the bright illumination circle is off center as

shown in the left image below.

9. If you chose to observe the adjustment via the live view of scan^R (see step 6), you will get
an image similar to the one inside the highlighted rectangle in the sketch above, which cor­responds to the field-of-view of the 2/3" CCD chip of the camera.

10. Iteratively center the bright disk by turning the two adjustment screws at the epi­fluorescence illuminator (see 8 in the photo in the next chapter).

34 Chapter 4 – System Assembly and Adjustment

5

7

8

4.5 Optimizing the Illuminator Tube Position

For an optimally homogeneous illumination of the specimen, it is necessary for the light cone leaving
the illuminator exit lens to match the position of the objective back aperture. Radial intensity gradients
will result in case the distance between exit lens and back aperture is severely off; the images are
brighter in the center than at the periphery.

Furthermore, in order to achieve maximum brightness, the exit plane of the light fiber has to be focused
onto the specimen plane. This depends on the distance between the fiber exit and the illuminator op­tics, in other words, the depth of insertion of the light fiber into the illuminator. It is roughly optimized
before delivery of the system by positioning the stopper ring 3 that sets the depth of penetration of the
fiber port adapter. Usually this will be sufficient for a good illumination. However, if it is obviously defo­cused the light fiber position has to be re-adjusted by slightly turning the stopper ring. To be able to do
that, the set screw 4 of the illuminator tube has to be slightly loosened by about half a turn.

8

6

1

4

3

2

1: illuminator tube containing the optical elements

2: light guide with port adapter

3: stopper ring to adjust depth of penetration

4: set screw to fix the light guide

5: flange for IX2 microscope frame

6: screw to fix the illuminator tube inside the flange

7: filter slider with dispersion filter

8: adjustment screws for centering the illuminated

field

The optical path length, which is (partly) determined by the penetration depth of the illuminator tube 1
into the microscope frame, is likewise crucial. The penetration can easily be changed after slightly loos­ening the fixing screw 6 in the flange 7. If the tube is either too far or not far enough inside the micro­scope, the overall brightness is reduced. However, the effects are hardly visible by eye, but they can be
monitored by carefully analyzing the images.

The following table lists the optimal distances to be adjusted for the different types of illuminators.
These distances are marked in the photo below. In the table you will find entries for the different groups
of objectives.

It is inconvenient to change the illuminator position every time if objectives of all three groups are fre­quently used: here, the distance of group B objectives can be chosen as a compromise.

Hardware Manual Chapter 4 – System Assembly and Adjustment 35

Standard and L-shape illuminators; the arrows mark the distances that have to be adjusted.

Optimal distance between flange

and illuminator tube end [mm]

Objectives Standard L-shape

A 231 41

B 221 31

C 211 21

The illumination will still be bright and homogeneous if, for example, a group C objective is
used but the illuminator is installed with the group A distance. However, for experiments
that are very critical with respect to the brightness of illumination, it might be useful to re­adjust the illuminator position.

Groups of objectives:

A LCPLFL 20x, LCPLFL 20x PH, PLAPO 1,25x, PLAPO 2x, UPLAPO 4x, UPLFL 4x, UPLFL 20x, UPLFL 4x P, UPLFL

20x P, UPLFL 4x PH, UPLFL 20x PH

B CPLFL 10x PH, LCPLFL 40x, LCPLFL 40x PH, LCPLFL 60x, LCPLFL 60x PH, PLAPO 40x, PLAPO 100x O3, PLFL

100x, SLCPLFL 40x, SLCPLFL 40x PH, UAPO 40x /340, UMPLFL 5x, UMPLFL 10x, UMPLFL 10x W, UMPLFL 20x,
UMPLFL 40x, UMPLFL 50x, UMPLFL 100x, UPLAPO 10x, UPLAPO 10x W, UPLAPO 10x PH, UPLAPO 20x,
UPLAPO 20x O, UPLAPO 20x PH, UPLAPO 40x, UPLAPO 60x, UPLFL 10x, UPLFL 10x PH, UPLFL 10x P, UPLFL
40x, UPLFL 40x P, UPLFL 40x PH

C all UIS2 objectives, APO100x OHR, LUMPLFL 40x W, LUMPLFL 40x W/IR, LUMPLFL 60x W, LUMPLFL 60x W/IR,

PLAPO 60x O3, PLAPO 60x O3PH, PLAPO 60X O/TIRFM-SP, UAPO 20x /340, UAPO 20x W/340, UAPO 40x W/340,
UAPO 40x OI/340, UMPLFL 20x W, UPLAPO 40x OI, UPLAPO 40x OI3PH, UPLAPO 60x W, UPLAPO 100x OI,
UPLAPO 100x OI3PH, UPLFL 60x OI, UPLFL 60x OI3PH, UPLFL 100x O, UPLFL 100x OI, UPLFL 100x OP, UPLFL
100x O3PH, XLUMPLFL20XW

The objectives highlighted bold are the most sensitive with respect to an optimal adjustment. If they are
frequently used, the distance should be set as corresponds to their group. If you cannot find a certain
objective in this list, please contact your local Olympus specialist.

36 Chapter 4 – System Assembly and Adjustment

4.6 Connecting the Controller of the Motorized Stage

Back panel of the Corvus stage controller

In this description it is assumed that the motorized stage is mounted on the microscope frame as de­signed by the manufacturer.

1. Make sure that the main switch is set to OFF.

2. Set the dip switch 2 in the red Configuration field at the back panel of the controller to ON

(up position). All other switches have to be in OFF position (down position). This sets the
Baud rate (data transfer rate) to the correct value.

3. Connect the joy stick with the male 9-pin Joystick plug.

4. Connect the female 13-pin Axis-2 plug with the y-axis motor (the one closer to the micro-

scope frame) of the motorized stage.

5. Connect the female 13-pin Axis-1 plug with the x-axis motor (the one closer to the edge of
the stage) of the motorized stage.

6. Connect the male 9-pin RS-232 / Host plug with a 9-pin PC serial ports (COM1 or PC­COM2).

7. Make sure that the main switch is set to OFF and connect Mains with the power outlet.

Do not use CTR-COM2 serial port of the cell^R Real-time Controller. It is the PC that acts
as host for the stage controller.

Hardware Manual Chapter 4 – System Assembly and Adjustment 37

4.7 PC-to-Controller Network Connection

Communication between the MSWindows imaging PC and the scan^R Real-time Controller is realized
through an ordinary, albeit internal, network connection via a network card.

The MSWindows status bar features an icon showing two little monitors. If the system works properly
you will see the mouse-over message shown in the screenshot below:

When double-clicking on the icon the CTR Status window will open. The Activity field counts the data
Packets being Sent and Received.

It is important that the configuration of the PC-to-controller network connection remains unchanged.
Otherwise the system may become disabled.

8. To control the settings, click on the Properties button in the CTR Status window to open
the CTR Properties window.

9. Select the Internet Protocol (TCP/IP) connection and click on the Properties button to open
the Internet Protocol (TCP/IP) Properties window.

10. The Use the following IP address has to be activated.

11. The IP Address must be 42 42 42 17.

12. The Subnet Mask must be 255 255 255 0.

38 Chapter 4 – System Assembly and Adjustment

13. Return to the CTR Properties window with OK.

14. Click on the Configure button and go to the Advanced tab of the Realtek window that

opens.

15. Select the Property Link Speed/Duplex Mode and then the Value 10Mbps/Full Duplex from
the shortlist. Confirm with OK.

Additionally, it is important the PC-to-controller network connection is NOT protected by a firewall.

16. To control this, go to the Advanced tab of the CTR Properties window and click on the Set­tings button.

17. Make sure that Off (not recommended) is activated in the Windows Firewall window.

The PC-to-controller network connection is not a computer safety risk and there is no rea­son for a firewall protection. This internal network connection is used only for the system
commands traffic and not a gateway to the internet or intranet.

Hardware Manual Chapter 4 – System Assembly and Adjustment 39

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