OLYMPUS SCAN User Manual [ru]
®
INSTRUCTIONS
AUTOMATED IMAGE ACQUISITION
HARDWARE
This instruction manual describes the hardware components controlled by the
Olympus
optimum performance and familiarize yourself fully with the use of these products, we
recommend that you study this manual thoroughly before operation. Together with
this manual, please also read the
manual as well as the manuals of all other devices controlled by this software in order
to understand general operation methods. Retain this manual in an easily accessible
place near a system for future reference.
Automated Image Acquisition Software. To ensure safety, obtain
Automated Image Acquisition Software
OLYMPUS SOFT IMAGING SOLUTIONS GMBH
Rupert-Mayer-Strasse 44
D-81379 München
Tel: +49 89 - 89 55 805 660
Fax +49 89 - 89 55 805 6606
Email: info@olympus-sis.com
www.olympus-sis.com
Imaging Excellence
We at Olympus Soft Imaging Solutions GmbH have tried to make the information in this manual as
accurate and reliable as possible. Nevertheless, Olympus Soft Imaging Solutions GmbH disclaims any
warranty of any kind, whether expressed or implied, as to any matter whatsoever relating to this manual, including without limitation the merchantability or fitness for any particular purpose. Olympus Soft
Imaging Solutions GmbH will from time to time revise the product described in this manual and reserves the right to make such changes without obligation to notify the purchaser. In no event shall
Olympus Soft Imaging Solutions GmbH be liable for any indirect, special, incidental, or consequential
damages arising out of purchase or use of this manual or the information contained therein.
No part of this document may be reproduced or transmitted in any form or by any means, electronic or
mechanical, for any purpose, without the prior permission of Olympus Soft Imaging Solutions GmbH.
© 2006 – 2008 by Olympus Soft Imaging Solutions GmbH. All rights reserved.
Manual version: 2.1, December 2008
XDeclaration of Conformity
Robert-Koch-Strasse 9
D-82152 Planegg
declares under its own responsibility that the following products
Imaging Station for Life Science Applications *
to which this declaration refers are in conformity with the
Low Voltage Directive 73/23/EEC
and the
EMC Directives 89/336/EEC and 93/68/EEC
and the following standards:
EN 61010-1: 2001 (Low Voltage)
EN 61326:1997 + A1: 1998 + A2: 2001 (EMC).
Michael Czempiel and Dr. Matthias Seel, Managing Directors
October 08, 2005
* The hardware of the basic scan^R system is identical to that of the basic cell^R system.
Hardware Manual Contents
Contents
1 Introduction and Overview ................................................................................1
1.1 Abstract ........................................................................................................2
1.2 Technical Support......................................................................................... 3
1.3 Symbols ........................................................................................................3
1.4 Checklist: Standard scan^R Components ...................................................4
2 Illumination System MT20..................................................................................5
2.1 Safety Precautions and Handling Instructions..............................................6
2.1.1 MT20 Safety Precautions .............................................................................6
2.1.2 Special Safety Precautions Regarding the Arc Burners ............................... 8
2.1.3 Handling Instructions and Recommendations .............................................9
2.1.4 Safety Labels ..............................................................................................10
2.2 Illumination System MT20 Overview ..........................................................11
2.2.1 The Front Panel...........................................................................................11
2.2.2 Top: the Burner Flap ...................................................................................12
2.2.3 Right Side: the Filter Wheel Flap ................................................................12
2.2.4 The Back Panel...........................................................................................13
2.2.5 Operation .................................................................................................... 13
2.2.6 Specifications and Technical Data of the MT20 .........................................14
2.2.7 Fuse Exchange ...........................................................................................15
2.2.8 Specifications / Technical Data of the Real-Time Controller Connectors .. 15
3 Epi-Fluorescence Illuminator ..........................................................................17
3.1 Features ......................................................................................................18
3.1.1 Light Guide Port.......................................................................................... 18
3.1.2 Adjustment Screws for Centering the Field of Illumination.........................19
3.1.3 Filter Slider for Dispersion Filter and Exciter Balancers .............................19
3.1.4 Light Sensor................................................................................................ 19
3.1.5 Flange for the IX81 Frame...........................................................................19
4 System Assembly and Adjustment .................................................................21
4.1 Overview: Connectivity of System Components ........................................ 22
4.2 Connecting the Modules of the scan^R Screening Station .......................23
4.2.1 Connecting the MT20 .................................................................................23
4.2.2 Mounting and Connecting the Epi-Fluorescence Illuminator .....................24
4.2.3 Connecting the Camera.............................................................................. 25
4.2.4 Connecting the Imaging PC to the Modules ..............................................25
4.2.5 Configuring the Imaging PC Network Connections.................................... 26
4.3 Preparing the Illumination System MT20.................................................... 27
4.3.1 Burner Installation and Exchange...............................................................27
4.3.2 Burner Alignment ........................................................................................ 28
4.3.3 Filter Exchange ........................................................................................... 31
4.4 Centering the Illumination ...........................................................................33
4.5 Optimizing the Illuminator Tube Position....................................................34
4.6 Connecting the Controller of the Motorized Stage.....................................36
4.7 PC-to-Controller Network Connection .......................................................37
Contents Hardware Manual Chapter 1 – Introduction and Overview 1
1 Introduction and Overview
Thank you very much for purchasing Olympus' state of art Screening Station for Life Science
and for your confidence in our products and services. It is Olympus main objective to provide you with
solutions able to meet your experimental demands and thus pave the way to your scientific success.
The hardware described here constitutes essential parts of the Olympus Screening Station
hardware components are intended to be controlled via the
image acquisition of biomedical samples. In combination with the
hardware is intended for the use in biomedical research.
Acquisition Software for automated
Acquisition Software the
. The
2 Chapter 1 – Introduction and Overview
The
Analysis Software, the Acquisition Software as well as the hardware
components of the Olympus Screening Station for Life Sciences are for research
use only.
1.1 Abstract
The Screening Station for Life Science scan^R is based on the hardware of the cell^R Imaging Station
and consists of the following hardware components:
— Illumination System MT20
— Illumination Coupling to the microscope (epi-fluorescence illuminator with light fiber)
— Real-time Controller board inside PC
— CCD camera
You will find a detailed description of these components, handling and alignment instructions as well as
advice how to maintain the performance of your system on the following pages. Keep in mind that all
the components are designed to work together as an integrated system. Taking away or replacing single components without prior consultation of Olympus Soft Imaging Solutions is strongly discouraged
and will most probably impair the performance of the system dramatically. Any damages to the system
due to such mishandling will not be covered by the guarantee.
All necessary tools for handling and alignment are shipped with the system.
The Imaging Station scan^R is compatible with the Olympus microscope IX81.
An additional installation of a standard Olympus epi-fluorescence Xe, Xe/Hg or Hg lamp housing e.g.,
U-LH100HGAPO, U-LH75XEAPO) is not possible because the microscope's fluorescence excitation
port is occupied by the OBS epi-fluorescence illuminator.
This instructions manual is for the hardware components of the Olympus Soft Imaging Solutions
Screening Station scan^R. To ensure safety and optimum performance as well as to familiarize yourself
with the use of the system we recommend you to study this manual thoroughly before operating the
system. Keep this manual in an easily accessible place near the Imaging Station for future reference.
The scan^R system meets the CE, UL and FCC requirements.
nd
IEC/EN 61010-1(2
ed.), UL61010-1,
EN61326 Class A, FCC Part 15 Class A (Illumination System MT20: class A and B).
Hardware Manual Chapter 1 – Introduction and Overview 3
1.2 Technical Support
If you find any information missing in this manual or you need additional support, please contact your
local Olympus representative.
1.3 Symbols
The following symbols are placed on the Illumination System MT20. Study the meaning of the symbols
and always use the equipment in the safest possible manner.
General symbols
Symbol Explanation
Carefully read the instructions on the label and the manual before use. Improper use
could result in personal injury to the user and/or damage the equipment.
The surface becomes hot and should not be touched with bare hands
Parts may be under high voltage and should not be touched.
The main switch is turned OFF.
The main switch is turned ON.
This symbol indicates separate collection of waste electrical and electronic equipment in
the EU countries.
Do not throw the equipment into the domestic refuse
Please use the return and collection systems available in your country for the disposal of
this product
4 Chapter 1 – Introduction and Overview
1.4 Checklist: Standard scan^R Components
Before assembly, please check whether you have the complete set of standard scan^R system components as listed below.
a Microscope IX81 with control unit IX2-UCB
b Illumination System MT20 consisting of:
— MT20 housing with integrated lamp house, filter wheel, shutter and attenuator
— short arc burner
— power cord
— Illumination System connector cord (blue RJ45 cable)
— Light sensor connector cord (see photo in Chapter 7, System Assembly and Adjustment)
— optical fiber (quartz light guide; see photo in Chapter 7, System Assembly and Adjustment)
— set of hex-wrenches
— cotton gloves
c Fluorescence filter set consisting of:
— Dichroic mirror and emission filter mounted in Olympus filter cube
— Excitation filter(s) to be mounted in the MT20 filter wheel
d MT20 epi-fluorescence illuminator to couple the illumination system to the microscope via the opti-
cal fiber
e CCD camera
— FireWire
— trigger cable
TM
(IEEE 1394) cable
f Motorized stage with controller
g cell^R imaging computer including
— cell^R Real-time Controller board
— FireWire
— Front panel with three digital I/O ports
— scan^R acquisition and analysis software installed
— computer monitor with cable
— 2 power cords
— software protection key (dongle)
TM
(IEEE 1394) card
h scan^R acquisition and analysis software on CD-ROM
i Manuals
j Z-drift control nit ZDC mounted on the IX81 frame (optional)
k Climate chamber (optional)
Hardware Manual Chapter 2 – Illumination System MT20 5
2 Illumination System MT20
This chapter refers only to the fluorescence Illumination System MT20. Before using this unit together
with the other system components, the microscope and associated devices, make sure that you have
carefully read and understood their respective manuals, and understand how the entire system should
be operated.
2.1 Safety Precautions and Handling Instructions..............................................6
2.1.1 MT20 Safety Precautions .............................................................................6
2.1.2 Special Safety Precautions Regarding the Arc Burners ............................... 8
2.1.3 Handling Instructions and Recommendations .............................................9
2.1.4 Safety Labels ..............................................................................................10
2.2 Illumination System MT20 Overview ..........................................................11
2.2.1 The Front Panel...........................................................................................11
2.2.2 Top: the Burner Flap ...................................................................................12
2.2.3 Right Side: the Filter Wheel Flap ................................................................12
2.2.4 The Back Panel...........................................................................................13
2.2.5 Operation .................................................................................................... 13
2.2.6 Specifications and Technical Data of the MT20 .........................................14
2.2.7 Fuse Exchange ...........................................................................................15
2.2.8 Specifications / Technical Data of the Real-Time Controller Connectors .. 15
6 Chapter 2 – Illumination System MT20
2.1 Safety Precautions and Handling Instructions
For Laboratory Use Only.
Danger: Ultraviolet radiation is emitted from this product. Avoid exposure. ALWAYS WARE
PROTECTIVE CLOTHING. EXPOSURE MAY CAUSE PREMATURE AGING OF THE SKIN
AND CANCER. ALWAYS WARE PROTECTIVE EYEWEAR; FAILURE TO DO SO MAY
RESULT IN SEVERE BURNS OR LONG TERM INJURE TO THE EYE. Never look directly
into the lamp. Exposure can cause eye and skin allergy and allergic reactions. Medications
or cosmetics may increase your sensitivity to ultraviolet radiation. Consult a physician before operating this product if you are using medications or have a history of skin problems
or believe yourself particularly sensitive to sunlight.
Always have the microscope's UV cut plate mounted as protection against UV radiation.
Color fading of specimens: This system features high excitation light intensity to ensure
bright observation of dim fluorescent specimens. Consequently, after longer periods of observations using high-power objectives, the colors of specimens will fade quicker than
usual, causing the intensity and contrast of fluorescent images to deteriorate. In such cases
it is advisable to reduce excitation light intensity to slow down color fading. To reduce the
light intensity use the built-in attenuator as explained in this manual.
Engage the shutter of MT20 as explained in this manual (or use the shutter of the microscope) even if you interrupt observation only for a short time.
2.1.1 MT20 Safety Precautions
a Always use a power cord provided or approved by Olympus Soft Imaging Solutions.
b Provide unimpaired access to the main power switch at the rear panel.
c No electrical cord should be connected or disconnected while the power switch is set on (“I”). Make
sure that the unit's main power switch is set off (“O”) before plugging or unplugging the power cord
to the power outlet.
d Always make sure that the grounding terminal of the Illumination System MT20 and the wall outlet
are connected properly. If the System is not grounded, Olympus Soft Imaging Solutions cannot warrant the electrical safety and performance of the system.
e When installing the Illumination System MT20 leave at least 100 mm free air space at the rear panel
and at least 50 mm at the sides and on top to allow air circulation. The Illumination System MT20
has an internal fan to cool the arc burner. The air outlet is located at the rear of the housing while
the inlet grid is at the bottom.
Hardware Manual Chapter 2 – Illumination System MT20 7
f The Illumination System MT20 must be placed on an even, stable and flat surface and standing on
its supports. Make sure that no cloth or paper can clog the air inlet slits at the bottom of the housing.
g The arc burner has to be exclusively one of the two types specified by Olympus Soft Imaging Solu-
tions. No other arc burner may be used with the Illumination System MT20.
h Do not open the burner flap of the Illumination System MT20 while the arc burner is turned on. First
turn off the arc burner by software and wait for at least 30 minutes while the cooling fan is operating. Set the main power switch on “O” (OFF) before opening the burner flap. The arc burner socket
and all parts in the vicinity of the burner will be extremely hot during operation and will cause thermal injury if touched.
i Never attempt to disassemble the Illumination System MT20 or open its housing. The internal power
supply units and electrical circuits contain high voltage components and can cause severe electrical
shock and thus are not serviceable by the user. Warranty will be lost if the housing is opened.
j Do not operate the Illumination System MT20 under environmental conditions other than specified
in this manual (see Chapter 2.2.6., Specifications and Technical Data).
k Do not look directly into the light emitted by the arc burner with unprotected eyes under any cir-
cumstances. The emitted light is very intense and has a very high UV-content which may cause irreversible damage to your eyes.
l Avoid direct exposure of unprotected skin to the light emitted by the arc burner. It can cause severe
burns.
m Direct exposure to the arc burner light may occur at the following positions:
— Illumination System’s housing at the fiber port if the fiber is removed
— Exit of the optical fiber
— Exit of the illumination coupling (epi-fluorescence illuminator)
— Beam path of the microscope (which upon proper usage is not accessible by the user)
— Exit of the objective (or at the nose piece, if no objective is mounted)
n If the equipment is used not as specified by this manual, the safety and performance of the equip-
ment may be impaired. In addition, the equipment may also be damaged and warranty may be lost.
Always use the equipment as outlined in this instruction manual.
8 Chapter 2 – Illumination System MT20
2.1.2 Special Safety Precautions Regarding the Arc Burners
a Handling the arc burner bulb is potentially dangerous. Wear a protective mask, leather gloves and a
thick long-sleeved shirt or coat during handling and disposal (see below).
b The arc burner bulb is under high internal pressure. Do not hit the arc burner bulb against anything
nor scratch it or apply excessive stress. Avoid vibrations. Otherwise the arc burner bulb could explode and cause injuries.
c For transport and storage always place the arc burner in the provided protective case.
d Take care to avoid leaving fingerprints or dirt on any part of the high pressure arc burners. If con-
taminated, clean by wiping gently with gauze. To remove fingerprints or oil stains, wipe with gauze
slightly moistened with lens cleansing fluid.
Since these fluids are highly inflammable, be careful to keep them away from open fire, heat or hot
surfaces and potential sources of electrical sparks, such as main switches.
e The arc burners have an average life time as specified in this manual (see Chapter 2.2.6, Specifica-
tions and Technical Data). When the arc burner hour counter (see Chapter 4.3.2, Burner Alignment)
gives a reading higher than the specified life time, replace the burner with a new one following the
instructions for replacement (see Chapter 4.3.1, Burner Installation and Exchange) and disposal of
used burners (see below). Arc burners that are used longer than the rated service life time will have
a reduced intensity and stability, are likely to fail and might explode because of the deteriorated
glass condition.
f Arc burners not in service must always be kept in the provided protective case.
g Hg/Xe burner bulbs contain poisonous mercury. They have to be treated with special care. If they
explode during service mercury gas might be dispersed in the environment.
h Hg/Xe burners must be treated as hazardous waste. Always follow national and local laws and
guidelines when disposing of Hg/Xe burners. It is recommended to commission a professional
waste disposal company with the disposal. Hand over the burner in its original packaging.
i Xe burners: It is recommended to commission a professional waste disposal company with the
disposal. Hand over the burner in its original packaging. Disposal without breaking the glass part
may result in burner bulb explosion. Follow the instructions below to prevent harmful repercussions.
— Ware protective clothing.
— Wrap the used lamp in a thick cloth.
— Break the glass part of the lamp into pieces using a hammer.
— Dispose of as industrial waste (except Hg/Xe burners, see above).
Hardware Manual Chapter 2 – Illumination System MT20 9
2.1.3 Handling Instructions and Recommendations
a The Illumination System MT20 is a precision instrument. Handle it with great care and avoid subject-
ing it to sudden or severe impact. Also connect each cable gently.
b The built-in ventilating fan and the built-in filter wheels may generate vibrations and sound. There-
fore it is recommended not to place the Illumination System MT20 on the same table as the microscope.
c With exception of the burner flap and the filter flap the Illumination System MT20 may only be
opened and disassembled by authorized service personal. Otherwise warranty is lost.
d Before using the Illumination System MT20 for the first time check that the burner is installed cor-
rectly and that all cords are connected correctly.
e Before opening the burner flap for replacement of the burner, turn it off by software (OBS System
Configuration) and wait for at least 30 minutes while the cooling fan is operating. Then set the main
power switch on “O” (OFF) and open the burner flap. (Do not open the burner flap of the Illumination
System MT20 while the arc burner is turned on.)
f Do not place the unit inside a climate chamber.
g Do not bend the optical fiber to a diameter of less than 45 cm. The optical fiber is fragile and may
brake if bent too strongly or if torsional stress is applied.
h Cover the ends of the optical fiber with the protection cover if they are not attached to the Illumina-
tion System MT20 or to the microscope.
i Always operate the Illumination System MT20 in an upright position. Never lay it on the side during
operation. This could cause the lamp to burst, damage the equipment and cause injury.
j Before transport of the system remove the lamp and store it safely in the provided protective case.
k Mind that turning the arc burner on and off repeatedly will shorten the life span.
l To clean the surface of the Illumination System MT20, use a lint-free, dry and soft cloth. Never at-
tempt to use organic solvents or strong detergents.
m Take care to avoid leaving fingerprints or dirt on the optical filters, the lenses at the ends of the light
guide or the optics of the illumination coupling. If contaminated, clean by wiping gently with special
optical cleansing tissue. To remove fingerprints or oil stains, wipe with special optical cleansing tissue slightly moistened with lens cleansing fluid. Use the cotton gloves when handling light filters.
* Since these fluids are highly inflammable, be careful to keep them away from open fire, heat or hot
surfaces and potential sources of electrical sparks, such as main switches.
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