Olympus IX2-DSU, BX-DSU Instruction Manual

DISK SCANNING BIOLOGICAL MICROSCOPE

Petition

Thank you for your adoption of OLYMPUS microscope, this time.

Please read this manual carefully in order to get optimum performance of this microscope

and for safety considerations.

When using microscope, please have this manual by you and keep it with care.

A

X6608

CONTENTS

Correct assembly and adjustments are critical for this system to manifest its full performance. If you are going to

assemble the microscope yourself, please read section 8, “ASSEMBLY” carefully.

INTRODUCTION - Be sure to read this section for safe

use of the equipment. -......................................................1

Caution ............................................................................................................... 1

Suggestions to unpacking ....................................................................... 1

Safety Caution ..................................................................................... 2

Symbol for safety considerations..............................................................................3

Warning labels .........................................................................................................4

When using the system ............................................................................ 5

Cleanup and storage................................................................................. 5

Caution....................................................................................................... 5

Manual conventions.................................................................................. 6

I. System Outline

1 SYSTEM OUTLINE....................................................... 1-1

1-1 Principle............................................................................................1-1

1-2 Features ............................................................................................ 1-2

1-3 System Configuration...................................................................... 1-3

1-3-1 In case of BX series.....................................................................................1-3

1-3-2 BX-DSU.......................................................................................................1-4

1-3-3 In case of IX series ......................................................................................1-5

1-3-4 IX2-DSU.......................................................................................................1-6

CONTENTS

II. DSU

2 SUMMARY OF OBSERVATION PROCEDURES ........ 2-1

3 DETAILS OF EACH OPERATION................................ 3-1

3-1 General Precaution for observation............................................... 3-1

3-2 Selection of fluorescent mirror unit............................................... 3-2

3-3 Table of Combination – Objective lens to each observation and

applicable disk ................................................................................. 3-5

3-4 Assignment of operating buttons on body (BX61, BX62 or BX61WI

only)................................................................................................... 3-7

3-5 Powering up ..................................................................................... 3-8

3-6 Focusing........................................................................................... 3-8

3-7 Changeover of visual mirror unit (front side)................................ 3-9

3-7-1 In case of BX ...............................................................................................3-9

3-7-2 In case of IX.................................................................................................3-9

3-8 Disk IN/OUT .................................................................................... 3-10

3-9 Shutter OPEN/CLOSE.................................................................... 3-10

3-9-1 In case of BX .............................................................................................3-10

3-9-2 In case of IX...............................................................................................3-10

3-10 Changeover of camera mirror unit (rear side)........................... 3-11

3-11 Filter change................................................................................. 3-11

3-12 Adjustment of field iris diaphragm.............................................3-12

3-13 Lamp centering ............................................................................ 3-13

3-14 Camera focus alignment ............................................................. 3-15

3-15 Power shutdown .......................................................................... 3-15

4 VARIETIES OF OBSERVATION METHODS ............... 4-1

4-1 Visual observation (BX)................................................................... 4-1

4-1-1 Simultaneous observation of reflected-light fluorescent and phase contrast 4-1

CONTENTS

4-1-2 Simultaneous observation of reflected-light fluorescent and transmitting

Nomarski differential interference.................................................................4-2

4-2 Visual observation (IX) ....................................................................4-4

4-2-1 Simultaneous observation of reflected-light fluorescence and phase contrast4-4

4-2-2 Simultaneous observation of reflected-light fluorescence and differential

interference (transmitting) ............................................................................4-5

4-3 Camera observation (BX-IX) ...........................................................4-6

4-3-1 Transmitting bright-field and transmitted light phase contrast observation ...4-6

4-3-2 Transmitting differential interference observation.........................................4-6

5 TROUBLESHOOTING GUIDE ..................................... 5-1

6 SPECTRAL CHARACTERISTICS OF FILTERS ......... 6-1

7 SPECIFICATIONS ........................................................ 7-1

7-1 BX Series ..........................................................................................7-1

7-2 IX Series............................................................................................ 7-2

8 ASSEMBLY................................................................... 8-1

8-1 System block diagram..................................................................... 8-1

8-1-1 In case of BX ...............................................................................................8-1

8-1-2 In case of IX.................................................................................................8-2

8-2 Installation of light illuminator........................................................ 8-3

8-2-1 In case of BX ...............................................................................................8-3

8-2-2 In case of IX.................................................................................................8-4

8-3 Installation of disk box .................................................................... 8-4

8-4 Removal of transportation lock ......................................................8-5

8-5 Installation of illuminator ................................................................8-6

8-6 Installation of lamp house............................................................... 8-6

8-7 Installation of mercury lamp ...........................................................8-7

8-8 Lamp power supply setting ............................................................8-9

CONTENTS

8-9 Installation of front side turret...................................................... 8-10

8-9-1 In case of BX .............................................................................................8-10

8-9-2 In case of IX...............................................................................................8-10

8-10 Installation of visual mirror unit (front side) ............................. 8-11

8-10-1 In case of BX ...........................................................................................8-11

8-10-2 In case of IX.............................................................................................8-12

8-10-3 Method to make optionnal fluorescent mirror unit.....................................8-13

8-11 IN/OUT of rear side turret ............................................................ 8-14

8-12 Installation of camera mirror unit (rear side) ............................ 8-14

8-13 Disk installation and change ...................................................... 8-15

8-14 Installation of light shielding tube (BX61, BX62, BX51 or BX52)8-16

8-15 Installation of UV-cut plate.......................................................... 8-17

8-15-1 In case of BX ...........................................................................................8-17

8-15-2 In case of IX.............................................................................................8-17

8-16 Installation of filter or shutter (In case of U-FWR or IX2-SHA) 8-17

8-17 Filter IN/OUT inside motorized filter wheel ............................... 8-18

8-18 Field iris diaphragm IN/OUT ....................................................... 8-19

8-19 Installation of camera adapter and filter.................................... 8-20

8-19-1 In case of DSU C-mount adapter.............................................................8-20

8-19-2 C-mount adapter for DSULAMBDA10 / C-mount adapter for Ludl............8-20

8-20 Installation of camera.................................................................. 8-22

8-21 DIP switch settings on control box (BX61, BX62 and BX61W1

only)................................................................................................. 8-23

8-22 Installation of motor drive board................................................ 8-23

8-23 Z board setting and installation.................................................. 8-24

8-23-1 In case of BX61 and BX62 .......................................................................8-24

8-23-2 In case of BX61W1 ..................................................................................8-25

8-23-3 In case of IX81.........................................................................................8-26

INTRODUCTION

1

Page

INTRODUCTION

-

Be sure to read this section for safe use of the equipment.

-

Caution

This manual – part or whole, shall not be used or reproduced without permission.

Suggestions to unpacking

Release of transportation lock for disk unit

When powering up the control box without removing

transportation lock knob, the disk unit may be

damaged.

1. Loosen two fixing screws (2) of access panel (1) on top of disk box,

using dedicated the Allen screwdriver that comes with microscope

frame as accessory and detach the access panel (1).

2. Loosen fixing screw (3) from hole located at left side of disk box,

using the Allen screwdriver and pull up the disk unit (4).

Pull up the disk unit carefully so as not to hit it inside

wall of disk box.

Note

Note

(1)

(2)

(3)

(4)

(9)

INTRODUCTION

2

Page

3. Loosen knob (5) of disk unit (at 2 places) and open disk protective

covers (6).

4. Loosen transportation lock knob (7) and remove it from the disk

unit (4).

Install the transportation lock knob you removed to the threaded

hole (8) of disk unit.

The transportation lock knob may be used for re-

transportation. Keep it with care.

When you continue to install disk, see Section 8-13 Disk

installation and change.

5. Return disk protective covers (6) to original position and tighten

knob (5) of disk unit (at 2 places) and fix the covers.

6. Align mount dovetail of disk unit (4) and mount dovetail (9) inside

the disk box and push the disk unit from top until it hits to dead

end.

7. Tighten fixing screw (3) with use of the Allen screwdriver and fix it.

8. Attach access panel (1) on top of disk box and tighten fixing

screws (2) (at 2 places) and fix it.

Safety Caution

l When you change disk, turn power of control box to OFF position and wait for one minute or more until the

turn of disk is completely stopped. The disk turns very rapidly so that they may be a danger to squeeze your

finger.

l When you change mirror unit for camera observation, change it after you turn the power of control box to

OFF position. During the change, it may be a danger to squeeze your finger if you accidentally touch the

changeover switch.

l Do not touch surface of disk with your hand. If the disk is blurred, observation image may be deteriorated.

l Do not touch lamp house mount adapter with your hand when the system is in use. As the adapter is

installed near the lamp house, the surface will get high temperature.

l Do not put power cord in the vicinity of lamp house. Lamp house will be of very high temperature. If power

cord touched the lamp house, the cord may be molten which would cause an electric shock.

Note

(5)

(7)

(6)

(7)

(5)

(8)

INTRODUCTION

3

Page

l Do not turn the lamp ON without installing lamp house to microscope. Ultraviolet ray of lamp is hazardous to

your eyes. Do not look at the light from lamp house directly.

l Do not look at excited light directly. When you use microscope, attach ultraviolet ray cutting plate and see

specimen and others through ultraviolet ray cutting plate for sure.

l When you change mirror unit at front side turret and rear side turret, observe the placement to the light path

in order of 1. fluorescent mirror unit and 2. reference mirror (No.1 position at each turret). If reference mirror

were set in front side turret and rear side turret, strong light would be applied to the specimen and it is

dangerous. (Regarding method of changeover for front and rear side turret, refer to Section 3-7

Changeover of mirror unit for visual observation (front side) and 3-10 Changeover of mirror unit for camera

observation (rear side).

Symbol for safety considerations

This system comes with the following symbols.

Understand the meaning of symbol and handle it safely.

Symbol Meaning

Do not touch with your hand as the surface is very hot.

It may cause a burn.

Be careful so as not to entangle your finger with disk or mirror unit for camera

observation.

Please read instruction manual definitely before you use the system.

In case of erroneous handling, it may cause injury of user or damage to

product.

Main switch is ON.

Main switch is OFF.

INTRODUCTION

4

Page

Warning labels

Warning label is attached to the place where caution is particularly required for operation. Observe the

instructions.

Lamp house mount adapter (Hot Surface caution)

Disk box (Entanglement caution)

When warning label is blurred or peeled off, contact our local sales office for change.

INTRODUCTION

5

Page

When using the system

l Handle this system with care so as not to give a shock as it is a precision instrument.

l This system requires independent fluorescent mirror unit for visual and camera observation respectively.

Install fluorescent mirror unit for visual observation to front side turret and install fluorescent mirror unit for

camera observation to rear side turret.

l Use high performance fluorescent mirror unit (HQ type) for camera observation in order to get optimum

optical performance.

l The fluorescent mirror unit – DSU-MRFPHQ – that comes with this system as accessory is for camera

observation.

Do not install it to front side turret for visual observation.

l Environment should be of room temperature at 10~35°C with relative humidity at 30~80%.

l Do not disassemble each section in vain as it may cause failure.

l Lamp house will get very hot. When installing, acquire adequate space in vicinity of lamp house (10cm or

more), particularly on top.

Cleanup and storage

l Never put dirt or fingerprint on surface of lens and filter, etc. In case of contamination, use gauze and wipe

out lightly. In case that the dirt or fingerprint does not come off with gauze, then, use mixed liquid (alcohol 3:

ether 7) to put in gauze and wipe out gently.

Liquid like ether or alcohol is very inflammable. Care must be taken when you handle

such liquid against fire and power switch ON-OFF procedure. Particularly, when using

ether or alcohol, pay utmost attention to ventilation of the room.

l When you clean other parts of the system, use a soft cloth dipped with diluted mild detergent to wipe each

section. Do not use organic solvent as it would deteriorate plastic parts or paint.

Caution

l If this system is used in other methods than those described in this manual, the safety is not guaranteed

and it may cause a failure or damage to the system. Use this system in accordance with instruction manual.

INTRODUCTION

6

Page

Manual conventions

This manual describes with use of the following manual conventions.

² Conventions of note, reference and point

Conventions Description

Indicates note to prevent injury of user and damage of product

(including home furniture, etc.).

Note

Typical note is indicated with

Note

.

Tip

Advice or hint for reference is indicated with

Tip

.

II..

SYSTEM OUTLINE / Principle

I.

System Outline

I.

1-1

Page

1 SYSTEM OUTLINE

Attaching this system to a microscope, it comprises a confocal microscope system without laser, but with use of

a white light source. It acquires a high contrast image through resolution enhanced in light axis direction very

rapidly.

In addition, Olympus motorized microscopes IX81, BX61WI, BX61 or BX62 and their related motorized units

may be controlled through a 3rd party software, which is designed for observation by CCD camera.

And the combination of such the software and the microscope may enable 3 dimensional observation, time-

lapse observation and so on.

The software controls changeover of confocal/non-confocal mode, ND filter synchronized to confocal or non-

confocal mode and filter cube inside the disk box in conjunction with motorized focusing control of motorized

microscope and thus, by changing wavelength through Z-drive of focusing control, it acquires images for myriad

observation.

1-1 Principle

Light emitted from light source of mercury or xenon

reflects at fluorescent mirror and enters rotary disk

in light path. The rotary disk has a pattern of linear

lines that provides the effect equivalent to pin hole

by high speed rotation. Light that passes through

the pattern further passes through objective lens

and goes to the specimen. Fluorescence emitted

from the specimen passes through the objective

lens again and forms an image on the rotary disk.

Among image formed, a portion of light that is in

focus will pass through the disk and, CCD camera

captures the image and the specimen image will be

displayed on the monitor.

Light
source

ND Filter

Objective lens

Imaging lens

Imaging lens

Rotary disk

CCD Camera

Monitor

Specimen

Light
illuminator

Fluorescent
Mirror unit

Disk box

Camera adapter

Illumination
tube

SYSTEM OUTLINE / Features

I.

System Outline

I.

1-2

Page

1-2 Features

● Confocal disk employed enables to acquire image of confocal effect.

● As while light source such as mercury or xenon is used as a light source instead of

laser, excited light of various wavelengths can be used by combination of excitation

filter and cube.

● In case of non-confocal observation by displacing the disk from light path, an image

of deep focal point without confocal effect can be acquired. In case of confocal

observation by placing the disk in light path, a sharp image of shallow focal point

can be acquired. Changeover of confocal to non-confocal can be done very easily.

SYSTEM OUTLINE / System Configuration

I.

System Outline

I.

1-3

Page

1-3 System Configuration

1-3-1 In case of BX series

Figure of BX series System Configuration

When you use this system in conjunction with microscope, you cannot integrate the

following units:

Motorized condenser (U-UCD8A) and motorized reflected light fluorescence (BX-RFAA

and BX-RLAA).

Motorized filter wheel (U-FWT and U-FWO)

When you integrate motorized revolving nosepiece, connect control box (IX2-UCB) with

use of connecting cable (U-REMMT).

Monitor

Computer

Cooled CCD Camera

Control box
(IX2-UCB)
DSU motor drive board
(DU-MTDRV) attached

Hand switch
(U-HSTR2)

Lamp
House

Microscope
(BX51, BX52, BX61, BX62,
BX51WI or BX61WI)

Light illuminator
for DSU BX
(DU-RFABX)

DSU Disk box
(DU-DBIX)

Motorized filter wheel
(U-FWR)

Stand for DSU BX
(DU-STBX)

DSU Hand switch
(DU-HSW)

Shutter and
Filter wheel

SYSTEM OUTLINE / System Configuration

I.

System Outline

I.

1-4

Page

Table of Instruction Manuals for Modules to be integrated

Instruction Manual Module name

SYSTEM MICROSCOPE BX51/BX52

MOTORIZED SYSTEM
MICROSCOPE

BX61/BX62

FIXED-STAGE UPRIGHT
MICROSCOPE

BX51WI

FIXED-STAGE MOTORIZED
UPRIGHT MICROSCOPE

BX61WI

CONTROL BOX FOR IX2 HAND
SWITCH

IX2-UCB
U-HSTR2

Cooled CCD Camera Manual for Cooled CCD Camera you may use

Computer/Monitor Manual for computer you may use

1-3-2 BX-DSU

BX-DSU includes the units, which have a mark "*" in its description on the above picture.

* DSU Hand switch

(DU-HSW)

* Disk control cable

* DSU motor drive board

(DU-MTDRV)

* DSU Disk box

(DU-DBIX)

DSU C-mount adapter

(DSU-CAD)

* Stand for DSU BX

(DU-STBX)

* Motorized filter wheel

(U-FWR)

* Light illuminator for DSU BX

(DU-RFABX)

SYSTEM OUTLINE / System Configuration

I.

System Outline

I.

1-5

Page

1-3-3 In case of IX series

Figure of IX Series System Configuration

When you use this system in conjunction with microscope (IX71 or IX81), you cannot

integrate with the following units:

Motorized fluorescent mirror unit cassette (IX2-RFACA) and motorized filter wheel (U-

FWT and U-FWO).

Monitor

Computer

Cooled CCD Camera

Control box
(IX2-UCB)
DSU motor drive board
(DU-MTDRV) attached

Hand switch
(U-HSTR2)

Shutter and
Filter wheel

Lamp
House

Microscope
(IX71, IX81)

Light illuminator
for DSU IX
(DU-RFAIX)

DSU Disk box
(DU-DBIX)

Motorized filter wheel
(U-FWR)

Stand for DSU IX
(DU-STIX)

DSU Hand switch
(DU-HSW)

SYSTEM OUTLINE / System Configuration

I.

System Outline

I.

1-6

Page

Table of Instruction Manuals for Modules to be integrated

Instruction Manual Module name

INVERTED RESEARCH
MICROSCOPE

IX71

MOTORIZED INVERTED
RESEARCH MICROSCOPE

IX81

CONTROL BOX FOR IX2 HAND
SWITCH

IX2-UCB
U-HSTR2

Cooled CCD Camera Manual for Cooled CCD Camera you may use

Computer/Monitor Manual for computer you may use

1-3-4 IX2-DSU

IX2-DSU includes the units, which have a mark "*" in its description on the above picture.

* DSU motor drive board

(DU-MTDRV)

* DSU Hand switch

(DU-HSW)

* Disk control cable

* DSU Disk box

(DU-DBIX)

DSU C-mount adapter

(DSU-CAD)

* Stand for DSU IX

(DU-STIX)

* Motorized filter wheel

(U-FWR)

* Light illuminator for DSU IX

(DU-RFAIX)

* Mirror unit cassette

IIII..

SUMMARY OF OBSERVATION PROCEDURES

II .

DSU

II .

2-1

Page

2 SUMMARY OF OBSERVATION

PROCEDURES

This section describes procedures for fluorescent observation by visual method followed by camera observation

by confocal mode.

Preparation

•Install fluorescent mirror unit and objective lens applicable to observation method.

•Do centering of mercury lamp or xenon lamp.

•Bring camera in focus.

Procedures

Where Ref.

Turn power switch of each system to ON position

and do initialization.

.......................... Power switch of each

system

3-5

Turn power switch of lamp to ON position and wait

until arc is stabilized. (5 to 10 minutes).

.......................... Main switch of lamp power 3-5

Set specimen on stage.

.......................... Lateral/Longitudinal feed

handle

-

Turn turret of front side and insert visual mirror unit

that fits to specimen into light path.

.......................... Visual mirror unit (front

side)

3-7

Turn turret of rear side and insert No.1 reference

mirror into light path

.......................... Camera mirror unit (rear

side) change button

3-10

Displace disk from light path.

.......................... Disk IN/OUT button 3-8

Put objective lens in light path and open shutter to

bring the specimen in focus.

.......................... Objective lens change

button, revolving
nosepiece, shutter
open/close knob or Electric
shutter or focusing handle
or coarse/fine handle

3-6
3-9

Adjust light intensity with use of

ND filter to put in or remove

from light path.

Filter change button 3-11

Adjust entire field of view to be uniformly bright.

.......................... Collector lens focusing

knob

3-12

Adjust field iris diaphragm.

Field iris diaphragm lever 3-12

Visual observation

(To next page)

Summary of observation procedures

II .

DSU

II .

2-2

Page

(From previous page)

Turn turret of rear side and put camera mirror unit

that suits to the specimen.

.......................... Camera mirror unit (rear

side) change button

3-10

Turn turret of front side and put No.1 reference

mirror into light path

.......................... Visual mirror unit (front

side)

3-7

Set camera setting for observation.

.......................... Control of camera -

Adjust light intensity with use of

ND filter to put in or remove

from light path.

Filter change button

Camera

observation

Put disk into light path.

.......................... Disk IN/OUT button 3-8

Adjust light intensity with use of

ND filter to put in or remove

from light path.

Filter change button

If necessary, set camera setting

to meet with observation.

Control of camera

Confocal

observation

During observation, use shutter for short interruption of observation.

Note

DETAILS OF EACH OPERATION / General Precaution for observation

II .

DSU

II .

3-1

Page

3 DETAILS OF EACH OPERATION

3-1 General Precaution for observation

1. Verify that input voltage/frequency to be used is within the range of that described

in identification plate of the system.

2. Verify that power cord as well as connecting cord is securely connected.

3. In case that transmitted light phase contrast observation or transmitted light DIC

(Differential Interference Contrast) observation only is required, make one place

where fluorescent mirror unit is not attached and do observation.

4. Use field iris diaphragm, squeezing it until it circumscribes to the filed of view and,

in case that it is out of center, adjust it with use of the Allen screwdriver.

5. Use immersion oil for objection lens of immersion type.

6. When you use objective lens of LCPlanFI series, attach a correction cap that

compensates thickness of container used for objective lens that is used for

observation. See instruction manual for IX2 series for kinds of correction caps

available.

7. Objective lens, UPlanApo40x (cover glass thickness offset 0.11~0.23), PlanApo40x

(0.11~0.23), UApo40x3/340 (0.11~0.23), UPlanApo60xW3 (0.15~0.20),

LCPlanFI40x (0~2.5) and 60x (0~2.5), all come with a correction ring; can

compensate deterioration of resolution due to variance of cover glass thickness.

Correction method

While turning correction ring, seek the position by adjusting

focus with use of fine-tuning handle and get best possible

resolution.

8. When a pause is required frequently during observation, use shutter (manual or

electric shutter).

(If mercury lamp is turned ON-OFF so frequently, it may deteriorate life of lamp).

9. Note to color deterioration on specimen

In case that observation is done with use of objective lens of high power, color

deterioration on specimen may occur earlier and contrast of fluorescent image may

be deteriorated. In such case, the color deterioration may defer if intensity of

excited light is lowered to a certain extent and a good fluorescent image may be

acquired. Use of ND filter is recommended to lower intensity of the excited light if it

does not bother the observation. Alternatively, use shutter so as not to apply light

source to specimen so often than it is necessary. Moreover, it can defer the color

deterioration if anti-color deterioration agent commercially available (DABCO) is

used. Particularly, it is recommended that anti-color deterioration agent is used with

high power observation.

DETAILS OF EACH OPERATION / Selection of fluorescent mirror unit

II .

DSU

II .

3-2

Page

Be careful that specimen of certain kind cannot use anti-color

deterioration agent.

3-2 Selection of fluorescent mirror unit

Select fluorescent mirror unit that has excitation to meet with coloring matter.

Do not use fluorescent mirror unit in combination with brightfield

of view mirror unit (U-MBF3). U-MBF3 is very bright and it may

hurt your eyes. If for any reason the mix use of mirror units is

required, use U-MBFL3 that ND filter is built in or insert ND filter of

3%.

Note to excitation bandwidth

A few sets of excitation filters combined for different bandwidth are

available. Typically, a set of wide bandwith (W) is used but, in case of

the followings, the use of a separate set may be better.

(1) In case that brightness of fluorescence is dark

(B,G excitation only) Change to super wide bandwidth (SW)

(In case of SWB2, fluorescence that comes from
substance other than fluorescent coloring matter may
become stronger.)

(2) In case fluorescence that comes from substance other than

fluorescent coloring matter is strong Change to narrow bandwidth (N)

(Brightness of fluorescence becomes slightly darker.)

Note

TIP

Note

DETAILS OF EACH OPERATION / Selection of fluorescent mirror unit

II .

DSU

II .

3-3

Page

Table of combination for mirror unit and dichroic mirror filter

Excitation

Method

Mirror Unit Dichroic Mirror

Excitation

Filter

Barrier Filter Fluorochrome

U-MWU2 BP330-385

U

U-MNU2

DM400

BP360-370

BA420

• Auto fluorescence observation

• DAPI Dyeing: DNA

• Hoechest 33258, 33342 Dyeing:
Chromosome

V U-MNV2 DM455 BP400-410 BA455

• Catecholamine observation

• serotonin observation

• tetracycline Dyeing: Bone, Teeth

U-MWBV2 BP400-440

U-MNBV2

DM455

BP420-440

BA475

BV

U-MCFPHQ DM450 BP425-445 BA460-510

• quinacrine, quinacrine mustard
Dyeing: Chromosome

• thioflavine S Dyeing: lymph cell

• acriflavine Dyeing: Nucleic acid

• CFP use

U-MGFPHQ DM485 BP460-480 BA495-540

U-MWB2 BP460-490

U-MNB2 BP470-490

B

U-MSWB2

DM500

BP420-480

BA520IF

U-MWIB2 BP460-490

U-MNIB2

DM505

BP470-490

BA510IF

IB

U-MYFPHQ DM505 BP490-500 BA515-560

• FITC Dyeing: Fluorescent
antibody test

• acridine orange Dyeing:DNA, RNA

• auramine Dyeing: TB bacteria

• EGFP, S65T, RSGFP use

• YFP use

U-MWG2 BP510-550

U-MNG2 BP530-550G

U-MSWG2

DM570

BP480-550

BA590

DSU-

MRFPHQ

DM560 BP535-555 BA570-620

IG

U-MWIG2 DM565 BP520-550 BA580IF

• rhodamine, TRITC Dyeing:
Fluorescent antibody test

• propidium iodide Dyeing: DNA

• RFP use

IY U-MWIY2 DM600 BP545-580 BA610IF

texas red Dyeing: Fluorescent
antibody test

DETAILS OF EACH OPERATION / Selection of fluorescent mirror unit

II .

DSU

II .

3-4

Page

Color Separation Filter Combinations

Excitation

Method

Mirror Unit Dichroic Mirror

Excitation

Filter

Barrier Filter Fluorochrome

U U-MNUA2 DM400 BP360-370 BA420-460

Observation of U-excited coloring
matter only when Dyeing U-excited
coloring matter and multi-Dyeing of
FITC.

U-MWIBA2 BP460-490

IB

U-MNIBA2

DM505

BP470-490

BA510-550

Observation of B-excited coloring
matter only when Dyeing B-excited
coloring matter or multi-Dyeing
TRITC or texas red.

Meaning of mirror unit name (Except HQ type)

Type

A

: For Color Separation

Excitation (U, V, BV, B, IB, G, IG, IY)

Bandwidth SW: Ultra wide band W: Wide band N:

Mirror unit

Universal

U - M N IB A 2

U-MGFPHQ and DSU-MRFPHQ come with this system as standard accessories.

As fluorescent mirror unit for camera observation, use HQ type.

DSU-MRFPHQ – accessory of this system – is dedicated for camera observation.

Note

DETAILS OF EACH OPERATION / Table of Combination – Objective lens to each observation and applicable

disk

II .

DSU

II .

3-5

Page

3-3 Table of Combination – Objective lens to each observation and

applicable disk

Reflected light fluorescence Differential interference

Objection lens

340 U, V, BV B, IB, G, IY

Phase

contrast

BX2 BX2WI* IX2

Applicable disk

UPlanApo 4x ––––

DSU-D1
DSU-D2

10x

*

–

DSU-D1
DSU-D2

20x

*

–

DSU-D1
DSU-D2

20xO – – DSU-D1

40x – – DSU-D3

40xOI3

*

–

DSU-D1
DSU-D2

60xW3 – DSU-D3

60xW/IR3 – DSU-D3

100xOI3

*

DSU-D4

UPlanFl 4x

*

–––

DSU-D1
DSU-D2

10x

*

–

DSU-D1
DSU-D2

20x

*

––

DSU-D1
DSU-D2

40x

*

– – DSU-D3

40xO – –

DSU-D1
DSU-D2

60xOI3 – DSU-D3

100xO3

*

DSU-D4

100xOI3 – DSU-D4

CPlanFl 10xPh

*

––

DSU-D1

UApo 20x3/340 –––

DSU-D1
DSU-D2

40x3/340 – – DSU-D3

40xOI3/340 – –

DSU-D1
DSU-D2

40xW3/340 – –

DSU-D1
DSU-D2

PlanApo 60xO3 – – – DSU-D3

DETAILS OF EACH OPERATION / Table of Combination – Objective lens to each observation and applicable

disk

II .

DSU

II .

3-6

Page

Reflected light fluorescence Differential interference

Objection lens

340 U, V, BV B, IB, G, IY

Phase

contrast

BX2 BX2WI* IX2

Applicable disk

LCPlanFl 20x – –

*

––

DSU-D3

40x –

*

– DSU-D4

60x – –

*

––

DSU-D4

LCAch 20x –

*

––– DSU-D3

40x – –

*

––– DSU-D4

LUMPLFL 40xW – –DSU-D3

40xW/IR – –DSU-D3

60xW – –DSU-D4

60xW/IR – –DSU-D4

XLUMPLFL 20xW –– –DSU-D5

* BX2WI can be used for differential interference contrast observation - the same combination as BX2 - provided

that it uses U-UCD8.

: Useable combination.

: Useable combination but it gets dark.

* : For phase contrast observation, objective lens for phase contrast is required.

– : Not useable or no applicable objective lens available.

DSU-D1 : Standard, for thick specimen

DSU-D2 : Standard, for thin specimen (comes with this system as accessory)

DSU-D3 : For WI 40x

DSU-D4 : For WI Standard 60x

DSU-D5 : For XLUMPLFL20x only

(DSU-D1/DSU-D2 should be used according to thickness of specimen.)

Plastic container (petri dish or flask) is not useful for differential

inteference observation.

Note

DETAILS OF EACH OPERATION / Assignment of operating buttons on body (BX61, BX62 or BX61WI only)

II .

DSU

II .

3-7

Page

3-4 Assignment of operating buttons on body (BX61, BX62 or BX61WI

only)

When IX2-BSW software boots up, microscope IX and its operation

buttons will be displayed on tab called [Frame SW]. When you use

BX61, BX62 or BX61WI, however, microscope does not appear.

Assignment of operating buttons for microscope – BX61, BX62 or

BX61WI as well as operating buttons on [Frame SW] tab are as

follows.

Not used

Focus UP button

Focus DOWN button

Brightness adjusting button (getting bright)

Brightness adjusting button (getting dark)

DETAILS OF EACH OPERATION / Powering up

II .

DSU

II .

3-8

Page

*1In case that control box (IX2-UCB) is of type 1, the identifying plate indicates IX2-UCB.

*

2

In case that control box (IX2-UCB) is of type 2, the identifying plate indicates IX2-UCB-2.

3-5 Powering up

1. Switch on the control box.

2. Switch on the lamp.

3. Switch on CCD camera.

3-6 Focusing

Use coarse/fine handle to bring the specimen in focus. In case of motorized microscope,

use focus handle unit or focus operating button on body to bring the specimen in focus.

Lamp ON/OFF button

Lamp set*

1

Not used*

2

F/C Change button

Focus retreat button

DETAILS OF EACH OPERATION / Changeover of visual mirror unit (front side)

II .

DSU

II .

3-9

Page

3-7 Changeover of visual mirror unit (front side)

3-7-1 In case of BX

Turn turret (1) located in front of light illuminator and insert mirror unit

desired or reference mirror into light path. Reference mirror to insert into

light path is normally put in No. 1 for use of camera observation.

In case that mirror unit name is put in a pocket of identifying

plate (2) of microscope frame, you can verify the name of mirror

unit.

When you insert reference mirror put in No.1 into light

path, verify that rear side turret is set to fluorescent mirror

unit.

3-7-2 In case of IX

1. Look at mirror unit indication (1) on surface of mirror unit cassette or

look at indication (2) at right side of turret and verify mirror unit

number that is currently inserted into light path.

In case that mirror unit name is put in a pocket of

identifying plate (3) of microscope frame, you can verify

the name of mirror unit.

2. Turn turret (4) so as to bring number of mirror unit that is to be used

in indicator of mirror unit cassette (1) or (2) and fix it at click position.

Reference mirror to insert into light path is put in No. 1 for use of

camera observation.

Turning turret in [+] direction, the number changes in

order of "1→2→3→4→5→6→1", and in [−] direction, it

changes in order of "1→6→5→4→3→2→1".

When you insert reference mirror put in No.1 into light

path, verify that rear side turret is set to fluorescent

mirror unit.

(1)

TIP

TIP

(1)

(2)

(3)

(4)

TIP

(2)

Note

Note

DETAILS OF EACH OPERATION / Disk IN/OUT

II .

DSU

II .

3-10

Page

3-8 Disk IN/OUT

When you wish to insert disk into light path, press left IN button of DSU hand

switch. Likewise, press right OUT button so that the disk would be displaced from

light path.

When disk comes into light path, LED (1) located at left side of

disk box would light in green and, when it is displaced from

light path, the LED would go off.

3-9 Shutter OPEN/CLOSE

3-9-1 In case of BX

When shutter knob (1) located at right lower side of turret in front of light

illuminator is moved left to mark

side, the shutter would open, and right to

mark

side, it would close.

3-9-2 In case of IX

When shutter knob (1) located at right side of visual mirror unit is moved

left to

side, the shutter would open, and right to side, it would

close.

(1)

(1)

Note

(1)

DETAILS OF EACH OPERATION / Changeover of camera mirror unit (rear side)

II .

DSU

II .

3-11

Page

3-10 Changeover of camera mirror unit (rear side)

Press button on hand switch to turn the turret and insert mirror unit desired or reference

mirror into light path. Reference mirror is put in No. 1.

Buttons to designate mirror unit are assigned at ex-factory as illustrated in left figure.

(Two buttons, one is for clockwise turn and the other for counter-clockwise turn.)

When you insert reference mirror put in No.1, verify that front side turret

is set to fluorescent mirror unit.

3-11 Filter change

Press button on hand switch to turn filter wheel and insert ND filter desired into light

path.

Buttons to turn filter wheel are assigned at ex-factory as illustrated in left figure. (Two

buttons, one is for clockwise turn and the other for counter-clockwise turn.)

Note

DETAILS OF EACH OPERATION / Adjustment of field iris diaphragm

II .

DSU

II .

3-12

Page

3-12 Adjustment of field iris diaphragm

1. Turn front side turret (1) and insert fluorescent mirror unit desired

into light path.

2. Turn rear side turret and insert reference mirror of No. 1 into light

path.

3. Set objective lens of 10x and set the specimen.

4. Move shutter knob (2) located at lower right side of turret to left

(

mark side) to open the shutter.

5. Use coarse/fine handle or, in case of motorized microscope, use

focus handle unit or focusing button on microscope frame to

bring the specimen in focus.

6. Pull out field iris diaphragm lever (3) located at side of

illumination tube and squeeze it to the smallest diameter.

7. Insert the Allen screwdriver that comes as accessory into

centering hole (4) of microscope frame located at side of

illumination tube (2 places) and adjust the axis of field iris

diaphragm image in a way that it comes in the same axis with the

filed of view.

8. Push in field iris diaphragm lever (3) and open field iris

diaphragm until it inscribes the field of view. In case that the axis

is out of center, do centering procedure again.

9. Push in field iris diaphragm lever (3) further and make the

aperture to circumscribe with field of view.

(In case of BX)

(In case of IX)

(1)

(1)

(2)

(3)

(4)

(2)

DETAILS OF EACH OPERATION / Lamp centering

II .

DSU

II .

3-13

Page

3-13 Lamp centering

Lamp centering should be done after arc image of lamp is

stablized after main switch was turned ON (5 to 10

minitues later after lamp ON).

In case that frost filter 32FR80 is used, displace it from

light path.

1. Move shutter knob (1) to right ( mark side) to close the

shutter.

2. Turn front side turret and insert fluorescent mirror unit of B or

IB excitation for visual observation into light path.

(In case that these mirror units are not installed, use other

fluorescent mirror unit. However, in case of fluorescent

mirror unit of U excitation, it is essentially required to observe

through ultraviolet light cutting plate.)

3. Turn rear side turret and insert reference mirror of No.1 into

light path.

4. Move shutter knob (1) to left (

mark side) to open the

shutter.

5. Put objective lens 10x into light path. Put centering target U-

CST on the stage and align center of view with cross line of

U-CST.

6. Turn revolving nosepiece and bring a place where the

objective lens does not exist into light path (objective cap

should also be displaced).

7. Pull out field iris diaphragm lever (2) (to make the view

squeezed).

8. Turn collector lens focusing knob (3) to project arc image on

U-CST. (A)

In case that the image is not projected, turn lamp centering

knob (4).

9. Turn lamp centering knob (4) and move arc image to center of

right (or left) half view. (B)

10. Insert the Allen screwdriver into mirror focusing screw (5)

located at back panel of lamp house and turns it to bring

mirror arc image in focus. (C)

(In case of BX)

(In case of IX)

TIP

(1)

(1)

(2)

(3)

(4)

(5)

DETAILS OF EACH OPERATION / Lamp centering

II .

DSU

II .

3-14

Page

11. Turn lamp centering knob (4) and overlap arc image and

mirror arc image. (D)

When you feel that the collector lens focusing knob (3) is

hard to turn as it is located far away, insert extention

handle U-CLA (available at option) into the knob.

Lamp centering is not required until next lamp change.

Stringent mirror centering

Position of mirror is fixed after adjustment at ex-factory. When you wish

to make stringent adjustment further, excersize the following procedures

besides the procedures described in previous section. Meanwhile, note

that, after adjustment, the state at ex-factory cannot be revived.

1. Peel off blind seals (6) attached on back panel of lamp house,

using tweezers (2 places).

2. Loosen screws under the seals with use of the Allen

screwdriver. By loosening these two screws, the lock of

mirror would be released.

3. Next, peel off blind seals (7) (2 places) so that mirror

centering holes would appear.

4. Using the Allen screwdriver, adjust centering of mirror arc

image through these holes.

TIP

TIP

TIP

(6)

(7)

(5)

DETAILS OF EACH OPERATION / Camera focus alignment

II .

DSU

II .

3-15

Page

3-14 Camera focus alignment

1. Bring a live image on monitor. Put objective lens 10x into light path

and displace the specimen from light path.

2. Press left IN button (1) of DSU hand switch and put the disk into light

path.

3. Stop the disk turning.

1) Switch off power of control box.

2) Pull out disk control cable (2) from disk box.

3) Switch on power of control box.

*

Upon completion of camera focus alignment, reverse the

procedures to go back to the original state.

4. Turn rear side turret and bring arbitrarily selected fluorescent mirror

unit or empty place into light path.

5. Turn front side turret and bring reference mirror of No.1 into light path.

6. Adjust transmitting light for enabling to see the strips of disk on

monitor.

7. Loosen fixing screw (3) located, using the Allen screwdriver.

8. Seeing live image on monitor, turn adjusting ring of mount adapter (4)

and bring strips of disk in focus.

9. Tighten the fixing screw (3) (at least one place) located at port, using

the Allen screwdriver.

3-15 Power shutdown

1. Switch off the lamp.

2. Switch off the control box.

3. Switch off the CCD camera.

(1)

(3)

(2)

(4)

VARIETIES OF OBSERVATION METHODS / Visual observation (BX)

II .

DSU

II .

4-1

Page

4 VARIETIES OF OBSERVATION

METHODS

4-1 Visual observation (BX)

This system can be used for not only reflected-light fluorescent observation but also it combines

transmitting bright-field observation, transmitted light phase contrast observation and transmitting

differential interference observation. Particularly, for specimen that color deterioration occurs earlier,

the color deterioration can be minimized by initial positioning through phase contrast observation or

differential interference observation. In addition, simultaneous observation of reflected-light

fluorescent combined with phase contrast observation or reflected-light fluorescent combined with

differential interference observation is possible, and it enables to observe which part of cell is emitting

fluorescence plainly.

4-1-1 Simultaneous observation of reflected-light fluorescent and phase

contrast

For phase contrast observation, condenser (U-PCD2) or universal condenser (U-UCD8)

dedicated for phase contrast and objective lens dedicated for phase contrast are

required.

1. Put dummy mirror unit (the place where fluorescent mirror unit is not installed) into

light path.

2. Set Ph indication equivalent to Ph indication of objective lens on phase contrast

turret.

3. Do centering between ring slit and phase plate.

4. Put mirror unit to observe excitation desired into light path and open shutter.

5. Adjust transmitting light intensity to get optimum brightness balance for fluorescent

and phase contrast and then, do the observation.

To adjust transmitting light intensity, use ND filter or brightness adjusting

button on microscope frame.

Regarding details of phase contrast observation, see instruction manual

of condenser for phase contrast or universal condenser.

TIP

TIP

TIP

VARIETIES OF OBSERVATION METHODS / Visual observation (BX)

II .

DSU

II .

4-2

Page

4-1-2 Simultaneous observation of reflected-light fluorescent and transmitting

Nomarski differential interference

Transmitting Nomarski differential interference observation requires the following units.

(1) Universal condenser (U-UCD8)

(2) Transmitting DIC slider (U-DICT, U-DICTS, U-DICTHR or U-DICTHC)

(3) Analyzer (U-AN or U-AN360-3)

(4) Revolving nosepiece or motorized revolving nosepiece for DIC.

The analyzer (U-AN or U-AN360-3) for simultaneous observation is put in

upper insertion port above the dichroic mirror in order to use reflected-

light fluorescene effectively.

If analyzer (U-ANT) is put in transmitting DIC slider, the fluorescent image

may get dark and, also the analyzer may be burnt.

1. Put dummy mirror unit (the place where fluorescent mirror unit is not installed) into

light path.

2. Adjust polarizer of universal condenser and make it in Cross-Nicol state.

3. Insert revolving nosepiece into transmitting DIC slider.

4. Turn turret of universal condenser and select turret of Nomarski prism that meets

with objective lens to be used.

5. Put the objective lens to use into light path.

6. Set specimen on stage and bring it in focus.

7. Adjust field iris diaphragm of transmitting illumination system (built-in the

microscope) and aperture of universal condenser.

8. Turn prism knob of transmitting DIC slider and adjust contrast of differential

interference image.

9. Put mirror unit to observe excitation desired into light path and open shutter.

10. Adjust transmitting light intensity so as to get optimum brightness for fluorescent

and differential interference image.

Regarding details of transmitting differential interference observation, see

manual of Universal condenser (U-UCD8).

TIP

TIP

VARIETIES OF OBSERVATION METHODS / Visual observation (BX)

II .

DSU

II .

4-3

Page

When change of reflected-light fluorescence and transmitting Nomarski

differential interference is frequently done and simultaneous observation

is also required, it is recommended that high abrasion-resistant analyzer

slider (U-ANH) is used instead of analyzer (U-AN).

When change of reflected-light fluorescence and transmitting Nomarski

differential interference is frequently done but simultaneous observation

is not required, it would be convenient if mirror unit (U-MDICT3) for

differential interference is attached at front side turret, instead of using

analyzer (U-AN or U-ANH).

If the fluorescent mirror unit is changed to differential interference mirror

unit, the analyzer comes into light path together so that the changeover is

easy.

TIP

VARIETIES OF OBSERVATION METHODS / Visual observation (IX)

II .

DSU

II .

4-4

Page

4-2 Visual observation (IX)

This system can be used for not only reflected-light fluorescent observation but also it combines

transmitting bright-field observation, transmitted light phase contrast observation and transmitting

differential interference observation. Particularly, for specimen that color deterioration occurs earlier,

the color deterioration can be minimized by initial positioning through phase contrast observation or

differential interference observation. In addition, simultaneous observation of reflected-light

fluorescent combined with phase contrast observation or reflected-light fluorescent combined with

differential interference observation is possible, and it enables to observe which part of cell is emitting

fluorescence plainly.

IX81 or IX71 provides lamp ON-OFF button. Use these buttons when you change to fluorescent

observation only.

4-2-1 Simultaneous observation of reflected-light fluorescence and phase

contrast

For phase contrast observation, any one of condenser IX2-LWUCDA, IX2-

LWUCD, IX-ULWCD or U-UCD8 and objective lens for phase contrast are

required.

1. Put dummy mirror unit (the place where fluorescent mirror unit is not installed) into

light path.

2. Set Ph indication equivalent to Ph indication of objective lens on phase contrast

turret.

3. Do centering between ring slit and phase plate.

4. Put mirror unit to observe excitation desired into light path and open shutter.

5. Adjust transmitting light intensity to get optimum brightness balance for fluorescent

and phase contrast and then, do the observation.

To adjust transmitting light intensity, use ND filter or green filter.

Alternatively, adjust brightness at microscope side.

Moreover, be careful that, if halogen lamp were used at low voltage

applied at lamp off time, the lamp is susceptible to shorten its life.

TIP

TIP

TIP

TIPTIP

VARIETIES OF OBSERVATION METHODS / Visual observation (IX)

II .

DSU

II .

4-5

Page

4-2-2 Simultaneous observation of reflected-light fluorescence and differential

interference (transmitting)

Differential interference observation requires the following units.

(1) One of condenser, IX2-LWUCDA, IX2-LWUCD or U-UCD8.

(2) One of differential interference slider, U-DICT, U-DICTS, U-DICTHC or U-DICTHR

(3) Analyzer, U-ANT or IX2-AN

The analyzer IX2-AN for simultaneous observation is put in lower

insertion port below the dichroic mirror in order to use reflected-light

fluorescene effectively.

If analyzer (U-ANT) is put in diffrential interference slider, the fluorescent

image may get dark and, also the analyzer may be burnt.

1. Put dummy mirror unit (the place where fluorescent mirror unit is not installed) into

light path.

2. Adjust polarizer of universal condenser and make it in Cross-Nicol state.

3. Insert revolving nosepiece into differential interference slider.

4. Turn turret of condenser and select turret of Nomarski prism that meets with

objective lens to be used.

5. Put the objective lens to use into light path.

6. Set specimen on stage and bring it in focus.

7. Adjust field iris diaphragm and aperture of condenser.

8. Turn prism knob of differential interference slider and adjust contrast of differential

interference image.

9. Put mirror unit to observe excitation desired into light path and open shutter.

10. Adjust transmitting light intensity so as to get optimum brightness for fluorescent

and differential interference image.

When change of reflected-light fluorescence and transmitting Nomarski

differential interference is frequently done but simultaneous observation

is not required, it would be convenient if mirror unit (IX2-MDICT) for

differential interference is attached at front side turret, instead of using

analyzer (IX2-AN). If the fluorescent mirror unit is changed to differential

interference mirror unit, the analyzer comes into light path together so that

the changeover is easy.

TIP

TIPTIP

VARIETIES OF OBSERVATION METHODS / Camera observation (BX-IX)

II .

DSU

II .

4-6

Page

4-3 Camera observation (BX-IX)

This system provides reflected-light fluorescent observation and also, by changeover of rear side

turret, it enables camera observation for transmitting bright-field observation, transmitted light phase

contrast observation and transmitting differential inteference observation.

4-3-1 Transmitting bright-field and transmitted light phase contrast observation

To execute camera observation for each transmitting, the system should be arranged for

visual observation as described in previous section, 4-1 and 4-2.

1. Upon setting for visual observation, put dummy mirror unit of rear side turret (the

place where fluorescent mirror unit or reference mirror is not installed) into light

path.

2. Turn front side turret and put reference mirror of No.1 into light path.

3. If necessary, adjust transmitting light intensity and then, enter camera setting.

4-3-2 Transmitting differential interference observation

To execute differential interference camera observation, attach IX2-MDICT to rear side

turret in case of BX or U-MDICT3 in case of IX.

Arrange the system for visual observation as described in previous section, 4-1 and 4-2.

1. Upon setting for visual observation, put IX2-MDICT of rear side turret into light path

in case of BX or U-MDICT3 in case of IX.

2. Turn front side turret and put reference mirror of No.1 into light path.

3. If necessary, adjust transmitting light intensity and the, enter camera setting.

TIP

TROUBLESHOOTING GUIDE

II .

DSU

II .

5-1

Page

5 TROUBLESHOOTING GUIDE

Depending upon how the system is used, it may not exhibit adequate performance of the system, though it may

not be a failure. When trouble occurs, exercise adequate countermeasure with reference to the following table.

In case that trouble has not been improved, please contact local Olympus representative for assistance.

Trouble Cause Countermeasure Ref.

Cable is not connected
correctly.

Connect cable correctly. 8-1

Disk unit is not correctly
inserted into disk box.

Insert disk unit into correct position firmly. 8-13

Interlock function is
activated as access panel
is not closed correctly.

Attach access panel firmly. 8-13

Disk does not turn.

• Stripes of disk appear on
the monitor.

• Confocal effect cannot
be acquired even if disk
were put into light path.

Transport lock is left as
attached.

Remove transport lock. 8-4

Mirror unit is about to
come off the cassette.

Remove turret from mirror unit cassette and
fix it to correct position firmly.

8-12Camera mirror unit does

not turn.

Electric filter wheel does
not turn.

Cable is not connected
correctly.

Connect cable correctly. 8-1

Power of camera is not
turned ON.

Switch on power of camera. 3-5

Shutter is entering light
path.

Displace shutter from light path. 3-9

Filter like that is entering
light path.

Displace unnecessary substance from light
path.

3-11

Specimen image does not
appear on the monitor.

Combination of visual
mirror unit and camera
mirror unit is incorrect.

Set visual mirror unit (front side) to
reference mirror and set camera mirror unit
(rear side) to mirror unit.

3-10

3-7

TROUBLESHOOTING GUIDE

II .

DSU

II .

5-2

Page

Trouble Cause Countermeasure Ref

Filter like that is entering
light path.

Displace unnecessary substance from light
path.

3-11

Exposure time of camera
is not correct.

Adjust to adequate time. Instruction

manual of

camera

used

Range of camera
brightness level is not
correct.

Adjust to adequate level. Instruction

manual of

camera

used

Confocal image is
extremely dark.

Combination of visual
mirror unit and camera
mirror unit is incorrect.

Put mirror unit that meets with purpose into
light path.

3-7

3-10

Centering of field iris
diaphragm is not done.

Do centering of field iris diaphragm
correctly.

3-12

Field iris diaphragm is
squeezed excessively.

Open aperture adequately. 3-12

Centering of lamp house is
not done.

Do centering of lamp house correctly. 3-13

Visual mirror unit is
positioned half way at
changeover.

Put mirror unit in click position firmly in
accordance with your purpose.

3-7

Field of view is obscured
or not evenly illuminated.

Shutter is positioned half
way.

Open shutter firmly. 3-9

Mirror unit is blurred. Clean adequately.

Cleanup and

Storage of

Introduction

Dust or dirt is visible in
filed of view.

Disk is dirty. Clean adequately.

Cleanup and

Storage of

Introduction

Exposure time of camera
is set to less than 200ms.

Set exposure time of camera to more than
200ms.

Instruction

manual of

camera

used

Confocal image is blinking.

Vibration at installation is
great.

Consult with our sales office. –

TROUBLESHOOTING GUIDE

II .

DSU

II .

5-3

Page

Trouble Cause Countermeasures Ref.

Ambient light (such as
fluorescent lamp) is
entering light path.

Turn off fluorescent lamp or cut ambient
light with use of specimen cover.

–

Exposure time of camera
is not correct.

Adjust to adequate time. Instruction

manual of

camera

used

Range of camera
brightness level is not
correct.

Adjust to adequate level. Instruction

manual of

camera

used

Non-fluorescent glass is
not used.

Use non-fluorescent glass. –

Flare is remarkable.

Thickness of cover glass is
not suitable.

Use cover glass of 0.17mm thick. –

Camera focus is not
correct.

Adjust camera focus. 3-14Image is not clear.

Correct disk is not used for
objective lens.

Use suitable disk. 8-13

3-3

SPECTRAL CHARACTERISTICS OF FILTERS

II .

DSU

II .

6-1

Page

6 SPECTRAL CHARACTERISTICS OF

FILTERS

RFPHQ DSU-MRFPHQ

0%

100%

300 400 500 600 700 800

Wavelength (nm)

Transmittance (%)

DM560

BA570-620HQ

BP535-555

SPECIFICATIONS / BX Series

II .

DSU

II .

7-1

Page

7 SPECIFICATIONS

7-1 BX Series

Item Specifications

Lens barrel magnification 1x

Number of views Visual observation side: FN22mm (In reflected illumination, peripheral

light intensity may be insufficient if objective lens power is of 20x or less)
Camera obervation side: FN11mm

Shutter Knob at front side turret cover.

Turret coupling system

Field iris diaphragm Field iris diaphragm built-in type (with centering function)

Aperture opening: Knob push-pull method
Centering: With use of the Allen screwdriver

Slider slot AN slot: 32X7.5mm through hole, with click (dummy when not used)

PO slot: 32X7.5mm through hole, with click (dummy when not used)

Disk Belt-drive system supported by spindle

DC motor
Electrically driven IN/OUT system (with hand switch)

Filter Electric filter wheel

Electric 6-hole turret system 1: Empty hole

2: ND25
3: ND12
4: ND6
5: ND3
6: ND1.5

Front turret Manual 6-hole turret system

1-hole fixed mirror accessory (DM680)

Mirror unit turret

Rear turret Electric 6-hole turret system

1-hole fixed mirror accessory (Reference mirror)

Stand Height adjustable

2 units provided.

Observation methods Camera observation

1) Incident-light fluorescent disk confocal

2) Incident-light fluorescent

3) Transmitting

Visual observation

1) Incident-light fluorescent

2) Incident-light fluorescent + Transmitting differential interference

3) Incident-light fluorescent + phase contrast

4) Transmitting

Dimensions of main unit

567mm(W)×386mm(D) ×221mm(H)

Weight 12.5kg

This device complies with the requirements of both directive 89/336/EEC concerning electromagnetic

compatibility and directive 73/23/EEC concerning low voltage. The CE marking indicates compliance with

the above directives.

SPECIFICATIONS / IX Series

II .

DSU

II .

7-2

Page

7-2 IX Series

Item Specifications

Lens-barrel magnification 1x

Number of views Visual observation side: FN22mm (In reflected illumination, peripheral

light intensity may be insufficient if objection lens is of 20x or less)
Camera observation side: FN11mm

Shutter Knob at front side turret cover.

Field iris diaphragm Field iris diaphragm built-in type (with centering function)

Aperture opening: Knob push-pull method
Centering: With use of the Allen screwdriver

Disk Belt-drive system supported by spindle

DC motor
Electrically driven IN/OUT system (with hand switch)

Filter Electric filter wheel

Electric 6-hole turret system 1: Empty hole

2: ND25
3: ND12
4: ND6
5: ND3
6: ND1.5

Front turret Manual 6-hole turret system

1-hole fixed mirror accessory (Reference mirror)

Mirror unit turret

Rear turret Electric 6-hole turret system

1-hole fixed mirror accessory (Reference mirror)

Stand Height adjustable

2 units provided.

Observation methods Camera observation

1) Incident-light fluorescent disk confocal

2) Incident-light fluorescent

3) Transmitting

Visual observation

1) Incident-light fluorescent

2) Incident-light fluorescent + Transmitting differential interference

3) Incident-light fluorescent + phase contrast

4) Transmitting

Dimensions of main unit

408mm(W)×386mm(D) ×221mm(H)

Weight 10kg

This device complies with the requirements of both directive 89/336/EEC concerning electromagnetic

compatibility and directive 73/23/EEC concerning low voltage. The CE marking indicates compliance with

the above directives.

ASSEMBLY / System block diagram

II .

DSU

II .

8-1

Page

8 ASSEMBLY

8-1 System block diagram

8-1-1 In case of BX

Microscope

BX51/

BX52/

BX61/

BX62/

BX51WI/

BX61WI

Moto rize d

Filter Wheel

U-FW R

DSU Disk Box

DU-DBIX

Mercury

lamp or

Xenon lamp

Halogen

Lamp

U-LH100-3

(High speed

Filter Wheel

& Shutter)

Round type

Connector

Lamp power

cable 2m

(High speed

Filter Wheel

& Shutter

Controller)

Disk Control Cable 2m

(High

speed

Filter

Wheel)

(Camer

a unit)

Round type

5-pin

Controller Power

cable 2m

Control Box

IX2-UCB

DSU Motor

Drive Board

DU-MTDRV

50-pin half

pitch

(Computer)

Computer power

cable 2m

Microscope Communication

cable 2m

(Camera

Power

Supply)

Camera power

cable 2m

D-SUB 15 pin

Camera power cable 3m

D-SUB 15 pin

Control cable 1.7m

Control cable 1.7m

20-pin Half pitch

Halogen lamp extension cable 1.7m

Microscope control cable 2m

Turret cable 2m

D-SUB 9 pin

20-pin Half pitch

Camera signal cable 4.5m

D-SUB 25-pin

Parallel port

Serial port

Round type

4-pin

Round type 4-pin

(1 side)

Round type 8-pin

(FW3)

Round type 8-pin

(RFACA/F1)

Filter wheel cable 2m

50-pin half pitch

(MAIN2)

20-pin

Half pitch

Merc ury l amp

power supply or

Xenon lamp

power supply

Lamp cable 2m

D-SUB 15-pin (A side)

D-SUB 15-pin (B side)

(Camera

signal

processin

g board)

Controller

Communica

tion Cable

1.8m

20-pin Half pitch

Halogen lamp cable 0.3m

Mous e

Display

cable

2m

Display Output

connector

Display Input

connector

DSU Hand Switch

DU-HSW

DSU Hand Switch

Cable 2m

20-pin Half pitch

Hand Switch Cable 2m

Hand Switch

U-HSTR2

Control box Power

Cable 2m

Display P ower

cable 2m

Keyboard

(Monitor)

ASSEMBLY / System block diagram

II .

DSU

II .

8-2

Page

8-1-2 In case of IX

Motorized

Filter Wheel

U-FWR

DSU Disk Box

DU-DBIX

Mercury

lamp or

Xenon lamp

Halogen

Lamp

U-LH100-3

(High speed

Filter Wheel

& Shutter)

Round type

Connector

Lamp power

cable 2m

(High speed

Filter Wheel

& Shutter

Controller)

Disk Control Cable 2m

(High

speed

Filter

Wheel)

(Camer

a unit)

Round

type

5-pin

Controller Power

cable 2m

Control Box

IX2-UCB

DSU Motor

Drive Board

DU-MTDRV

50-pin

half pitch

36-pin

half pitch

(Computer)

Computer power

cable 2m

Microscope Communication

cable 2m

(Camera

Power

Supply)

Camera power

cable 2m

D-SUB 15 pin

Camera power cable 3m

D-SUB 15 pin

Control cable 1.7m

Control cable 1.7m

20-pin Half pitch

Halogen lamp extension cable 1.7m

Microscope control cable 2.2m

Turret cable 2m

D-SUB 9 pin

20-pin Half

pitch

Camera signal cable 4.5m

D-SUB 25-pin

Parallel port

Serial port

Round type

4-pin

Round type 4-pin (1 side)

Microscope control cable 1.2m

Round type 8-pin (FW3)

Round type 8-pin

(RFACA/F1)

Filter wheel cable 2m

50-pin half pitch (MAIN2)

36-pin half pitch (MAIN3)

20-pin

Half pitch

Merc ury l amp

power supply or

Xenon lamp

power supply

Lamp cable 2m

D-SUB 15-pin (A side)

D-SUB 15-pin (B side)

(Camera

signal

processin

g board)

Controller

Communication

Cable 1.8m

20-pin Half pitch

Halogen lamp cable 0.3m

Display

cable 2m

Display Output

connector

Display Input

connector

DSU Hand Switch

DU-HSW

DSU Hand Switch

Cable 2m

20-pin Half pitch

Hand Switch Cable 2m

Hand Switch

U-HSTR2

Control box Power

Cable 2m

Display P ower

cable 2m

Mouse

Keyboard

(Monitor)

Microscope

IX71/

IX81

ASSEMBLY / Installation of light illuminator

II .

DSU

II .

8-3

Page

8-2 Installation of light illuminator

8-2-1 In case of BX

1. Remove caps of mounting screws (2) on top of light illuminator (1)

with use of tweezers.

2. When using BX61, BX62, BX51 or BX52, put arm lift kit (W I-

ARMAD) on the arm and fix mounting screws (3) (4 pieces) to the

light illuminator firmly, using dedicated

the Allen wrench that

comes with kit.

3. When mounting the light illuminator, put it over slightly forward

position without aligning mounting screws (2) with mounting holes

(4) of microscope body.

4. Next, pressing the light illuminator towards right side and press it

towards rear side so that it would be positioned in correct

mounting position.

5. While pressing the light illuminator towards rear side, fix mounting

screws (2) (4 pieces) to the light illuminator by turning the

dedicated

the Allen wrench that comes with kit in clockwise

direction.

6. Attach caps of mounting screws (2) to their original positions.

7. When using BX61, BX62, BX51 or BX52, revolving nosepiece

adapter (DSU-READ) must be attached as follows.

1)

Loosen fixing screw (5) of microscope frame with use of the

dedicated the Allen screwdriver.

2) Align revolving nosepiece adapter (6) with mount dovetail

from front side of microscope frame and push it and then,

tighten fixing screw (5).

3) Loosen fixing screw (7)

located at right side of revolving

nosepiece adapter, using the Allen screwdriver.

4) Align revolving nosepiece (8) with mount dovetail from front

side of microscope frame and push it and then, tighten fixing

screw (7).

(2)

(4)

(1)

(3)

(5)

(6)

(7)

(8)

ASSEMBLY / Installation of disk box

II .

DSU

II .

8-4

Page

8-2-2 In case of IX

1. Loosen fixing screws (1) (2 places) that fix light

illuminator to microscope frame, using the Allen

wrench.

2. Insert light illuminator (2) into mounting hole (3).

Observe that pin at lower part of light illuminator

is aligned with mounting hole slot.

3. Tighten fixing screws (1) for light illuminator

and fix the light illuminator (2 places).

8-3 Installation of disk box

1. Loosen fixing screws (1) (2 places), using the Allen

screwdriver.

2. Insert disk box (2) into light illuminator, observing pin

position, and push it until it hits the dead end.

3. Tighten fixing screws (1) (2 places), using the Allen

screwdriver.

4. Loosen fixing screw (3) of stand that comes as

accessory and put the stand underneath the

observation tube of disk box.

Stand should be attached for sure to

prevent upside down.

5. Extend the stand to apply fixing place to observation

tube and tighten fixing screw (3).

Note

(1)

(both sides)

(3)

(2)

(2)

(3)

(1)

ASSEMBLY / Removal of transportation lock

II .

DSU

II .

8-5

Page

8-4 Removal of transportation lock

This work should be done before connecting

cable of disk box.

1. Loosen screws (2) (2 places) located on top of disk box to

fasten at front side of access panel to microscope frame and

detach the access panel (1).

2. Loosen fixing screw from hole (3) located at left side of disk

box and pull the disk unit (4) upward.

Pull up the disk unit carefully so as not to hit it

inside wall of disk box.

3. Loosen knobs (5) (2 places) of disk unit and open disk cover

(6).

4. Loosen transportation lock knob (7) and remove it from disk

unit (4). Attach the transportation lock knob you just removed

to threaded hole (8) of the disk unit.

Keep transportation lock knob with care as it

may be used for transportation again.

When you continue to install disk, see 8-13 Disk installation

and change.

5. Return disk cover (6) to original position and fix it by

tightening disk unit knobs (5) (2 places).

6. Align disk unit (4) with mount dovetail (9) of disk box and

push it from top to dead end.

7. Tighten fixing screw (3), using the Allen screwdriver.

8. Attach access panel (1) on top of disk box and tighten fixing

screws (2) (2 places).

Note

Note

Note

(1)

(2)

(3)

(5)

(7)

(6)

(7)

(5)

(8)

(9)

(4)

ASSEMBLY / Installation of illuminator

II .

DSU

II .

8-6

Page

8-5 Installation of illuminator

1. Loosen fixing screws (1) (2 places) that fix disk box to microscope

frame, using the Allen screwdriver.

2. Insert illuminator (2) into disk box (3) and push it to the dead end. At

this time, attach it in a way that FS unit faces towards observer.

3. Tighten fixing screws (1) (2 places), using the Allen screwdriver.

4. Loosen fixing screw (4) of stand and put stand that comes as

accessory underneath the illuminator.

Stand should be attached for sure to prevent upside

down.

5. Extend the stand to apply fixing place to illuminator and tighten fixing

screw (4).

8-6 Installation of lamp house

1. Loosen fixing screws of lamp house mount adapter (1) (2

places), using the Allen screwdriver.

2. Insert lamp house (2) into lamp house mount adapter of light

illuminator (3) and push it to the dead end.

3. Tighten fixing screws (1) (2 places), using the Allen

screwdriver.

Note

(4)

(2)

(3)

(1)

(2)

(3)

(1)

ASSEMBLY / Installation of mercury lamp

II .

DSU

II .

8-7

Page

8-7 Installation of mercury lamp

1. Loosen socket fixing screw (1), using the Allen screwdriver.

2. Hold upper part of lamp house and pull it vertically to detach socket

section.

If you hold centering knob (2), it may cause a failure.

3. Place socket section upside down as illustrated.

At ex-factory, a support is provided for transportation. When

changing lamp, old lamp is yet installed. When installing lamp

newly or changing lamp, loosen fixing screws (3) (2 places) that

hold the support or old lamp.

4. Install mercury lamp designated to mounting socket firmly, observing +

polarity to upper base and – polarity to lower base.

Lamp to use should be USH102D (made by Ushio Electric)

or HBO103W/2 (made by OSRAM).

Care must be taken so as not to attach finger mark or dirt

on lamp. If the surface of lamp is contaminated, it may burst

due to distotion of glass. If contaminated, use a cloth like

gauze dipped in mixture of alcohol (3): ether (7) and wipe

out the surface of lamp carefully.

5. Return socket section that the lamp is attached to original position and

tighten fixing screw (1).

Socket section must be lowered vertically, aligning lamp

house outer side and socket section outer side.

(1)

(2)

(3)

(4)

Note

TIP

Note

Note

ASSEMBLY / Installation of mercury lamp

II .

DSU

II .

8-8

Page

Life meter setting

1. Reset button (1) is located at front panel. Press center part (2) of this

button and make the display to 000.0.

Display – 000.0 means time. Life of lamp – USH102D is 200

hours, while HBO103W/2 is 300 hours. When 200.0 is displayed

for USH102D, replace the lamp. Likewise, when 300.0 is

displayed for HBO103W/2, replace the lamp.

Change of mercury lamp

l

Life of mercury lamp is either 200 or 300 hours. Lamp may burst when the life

of lamp is over. When you used 200 hurs (USH102D) or 300 hours

(HBO103W/2), replace the lamp with new one for safety.

l

For safety purpose, wait about 10 minutes after power of lamp was turned off

and see if the lamp and lamp house have been duly cooled down. Then, verify

that main switch is turned OFF and, after output connector is detached from

lamp house, remove the lamp.

See 8-7 Installation of mercury lamp for method of change.

l

Upon completion of lamp change, set life meter to 000.00 as explained above.

(1)

(2)

TIP

ASSEMBLY / Lamp power supply setting

II .

DSU

II .

8-9

Page

8-8 Lamp power supply setting

Cord or cable is weak against bend or twist. Care must be

taken so as not to apply excessive force.

When connecting power cord, turn main siwtch to mark

side (OFF).

Use power cord that comes our accessory for sure.

1. Verify that voltage and frequency of input power are within the range as

shown in identifying plate (1).

(The 100 V system can be used with voltages in the 100 to 120 V range

and 200 V system can be used with voltages in the 220 V to 240 V range,

both with frequencies of 50 or 60 Hz.)

2. Connect connecting cord to connector (2) of power supply unit.

3. Install power cord to input receptacle (3) of power supply unit and insert

power plug (4) into power consent (5).

Power cord should be connected to 3-core consent with

earth terminal. When the earth of consent is not connected,

the electric safety performance intended by our company

may not be acquired.

(1)

(2)

(3)

(4) (5)

ASSEMBLY / Installation of front side turret

II .

DSU

II .

8-10

Page

8-9 Installation of front side turret

8-9-1 In case of BX

1. (Removal of turret). Loosen fixing screw (1) from hole (1) located at

right side of light illuminator and pull out turret (2) forward.

2. (Installation of turret). Align mount dovetail (3) of light illuminator and

mount dovetail (4) of turret and push the turret from front side gently

until it hits to the dead end.

3. Tighten fixing screw (1), using the Allen screwdriver.

8-9-2 In case of IX

When you remove cassette during operation, close option shutter for sure

or turn power of light source to OFF position.

If you remove mirror unit cassette while the shutter is open, it is dangerous

as the excited light would leak.

1. (Removal). Loosen fixing screw (1) of mount dovetail for cassette, using

dedicated the Allen screwdriver and push out the cassette (2) to arrow

direction.

2. (Installation). Align mount dovetail located at bottom of mirror unit

cassette and insert the cassette from left side and push it until it hits to

dead end.

3. Tighten fixing screw (1) of mount dovetail for cassette, using the Allen

screwdriver.

(1)

(2)

(1)

(2)

(3)

(4)

ASSEMBLY / Installation of visual mirror unit (front side)

II .

DSU

II .

8-11

Page

8-10 Installation of visual mirror unit (front side)

8-10-1 In case of BX

1. Turn turret upside down and loosen fixing screw (1), using dedicated the

Allen screwdriver and remove dummy mirror unit (2) (remove the dummy

mirror unit only where a mirror unit is to be installed from now on).

When mirror units already exist inside the cassette, be

careful so as not to touch dichroic mirror or excitation filter

with your finger.

Reference mirror of adjustment completed is attached at

No.1 position. Do not change the position of this reference

mirror.

2. Insert mirror unit (3) to be used into mount dovetail (4) of mirror unit and

push it until it hits to dead end and then, tighten fixing screw (1), using The

Allen screwdriver.

If the fixing screw (1) is loosened, it may interfere inside

turret cover and turret may not be able to turn. Tighten the

fixing screw even if mirror unit does not exist.

3. Verifying mount dovetail NO (4), insert identifying plate of mirror unit in

reverse way into the same number of display pocket (5) located at front of

turret.

In case that the identifying pocket for turret is used as

indicator for name of objective lens, it cannot be used as

indicator for mirror unit.

Note

Note

Note

Note

(1)

(3)

(4)

(5)

(2)

ASSEMBLY / Installation of visual mirror unit (front side)

II .

DSU

II .

8-12

Page

8-10-2 In case of IX

1. Remove cover (1) that is sucked with magnet from mirror unit cassette.

When mirror units already exist inside the cassette, be

careful so as not to touch dichroic mirror or excitation filter

with your finger.

2. Turn turret (2) of mirror unit cassette manually and align mirror unit

mounting position with mirror unit mounting hole.

Reference mirror of adjustment completed is attached at

No.1 position. Do not change the position of this reference

mirror.

3. Loosen fixing screw (3) of mirror unit mount dovetail for mirror unit cassette,

using dedicated the Allen screwdriver.

4. Displace dummy mirror unit (4) little by little circumferentially and pull it

towards outside of turret and detach it by pulling upward.

In case of reflected-light fluorescence observation, attach

dummy mirror unit for sure to the place where mirror unit is

not installed. In case of transmitting observation, dummy

mirror unit is not required. However, the fixing screw (3) for

mount dovetail should be tightened at the place where

dummy mirror unit is not installed. If this fixing screw is

loosened, head of screw may interfere when turret turns.

5. Insert mirror unit (5) into mirror unit mount dovetail (6) and push it until it

hits to dead end and then, tighten fixing screw (3), using dedicated the

Allen screwdriver.

6. Attach turret cover (1), aligning it with well of mirror unit cover.

7. Insert identifying plate for mirror unit, corresponding to number of turret

that comes into light path into display pocket of microscope frame.

When display pocket of microscope frame is used for

indicator of objective lens, it cannot be used as indicator of

mirror unit.

Note

Note

Note

(1)

(3)

(4)

(2)

(3)

(6)

(5)

Note

ASSEMBLY / Installation of visual mirror unit (front side)

II .

DSU

II .

8-13

Page

8-10-3 Method to make optionnal fluorescent mirror unit

You can make optional fluorescent mirror unit by attaching mirror unit frame

U-MF2 to commercially available barrier Filter, excitation filter or dichroic

mirror.

Dimensional conditions for optical parts

• Barrier Filter

• Excitation filter

mm25

0.2

0.1--

f

Max. Height 6mm

• Dichroic filter

mm3826

0.3-

-0.1

0.3

0.1

´

-

-

Thickness 1±0.05mm

When you change dichroic mirror, be careful so as not to contaminate it

with your finger print.

TIP

Barrier Filter (commercial available)

Dichroic mirror
(Commercial available)

Interference film surface

Excitation filter
(commercial available)

Note

ASSEMBLY / IN/OUT of rear side turret

II .

DSU

II .

8-14

Page

8-11 IN/OUT of rear side turret

When you install or detach the turret, you must do the works

after you turn the power of control box.

1. (Removal). Loosen screws (2) (2 places) at rear side of access panel (1) on

top of disk box, using dedicated the Allen screwdriver and remove the access

panel.

2. Loosen fixing screw from hole (3) located at left side of disk box, using The

Allen screwdriver and hold the handle (4) of turret and pull it upward.

Pull out the turret gently so as not to hit mirror unit with inside

wall of disk box.

3. (Installation). Align mount dovetail of turret and mount dovetail of disk box and

push the turret from top until it hits to dead end.

4. Tighten fixing screw (3), using the Allen screwdriver.

5. Attach access panel (1) on top of disk box and tighten fixing screws (2) (2

places), using the Allen screwdriver.

8-12 Installation of camera mirror unit (rear side)

1. Loosen fixing screw (1), using the Allen screwdriver and

remove dummy mirror unit (2).

When mirror units already exist inside the

cassette, be careful so as not to touch dichroic

mirror or excitation filter with your finger.

Reference mirror of adjustment completed is

attached at No.1 position. Do not change the

position of this reference mirror.

2. Insert mirror unit (3) to be used into mount dovetail (4) of

mirror unit and push it until it hits to dead end and the, tighten

fixing screw (1), using the Allen screwdriver.

Note

Note

Note

Note

(1)

(2)

(3)

(4)

(1)

(2)

(3)

(4)

ASSEMBLY / Disk installation and change

II .

DSU

II .

8-15

Page

For camera observation, use fluorescent mirror

unit of HQ type.

The fluorescent mirror unit type DSU-MRFPHQ

– accessory of this system – is dedicated for

camera observation.

8-13 Disk installation and change

For disk installation or change, you must do the works after you

verify that the disk entered into light path and then, you turn the

power of control box.

1. Loosen screws (2) (2 places) at front side of access panel (1) located

on top of disk box, using the Allen screwdriver and remove the

access panel.

2. Loosen fixing screw from hole (3) located at left side of disk box,

using the Allen screwdriver and pull disk unit (4) upward.

Pull out disk unit gently so as not to hit disk unit with

inside wall of disk box.

3. Loosen knob of disk unit (5) (2 places) and open disk cover (6).

4. In case that transportation lock knob is attached instead of disk,

remove the transportation lock knob first. (Regarding transportation

lock knob, see 8-4 Removal of transportation lock.) Pressing the

edge of disk, turn rotary shaft (7) in clockwise direction, using the

Allen screwdriver and remove disk attached to the disk unit (4).

5. Holding edge of disk, get out the disk that you are going to install

from the package without removing wrapper.

Note

Note

(1)

(2)

(3)

Note

(8)

(4)

ASSEMBLY / Installation of light shielding tube (BX61, BX62, BX51 or BX52)

II .

DSU

II .

8-16

Page

6. Set the disk, putting the side attached with name seal (dark blue

side) down and, holding edge of disk, turn rotary shaft (7) in counter-

clockwise direction to screw in to the end, using the Allen screwdriver.

After fixing, remover the wrapper.

If you install disk upside down, cover (6) would not

close. If you attempt to close cover (6), it would

damage surface of disk. Therefore, attach the disk in

correct direction.

7. Return disk cover (6) to original position and tighten knob (5) of disk

unit (2 places) and fix the cover.

8. The disk removed should be kept in the packet. Transport lock knob

should be attached to the disk unit.

9. Align dovetail of disk unit (4) and dovetail of disk box (8) and push it

from top to dead end.

10. Tighten fixing screw (3), using the Allen screwdriver.

11. Attach access panel (1) on top of disk box and tighten fixing screws

(2) at two places, using the Allen screwdriver.

8-14 Installation of light shielding tube (BX61, BX62, BX51 or BX52)

1. Remove front side turret. For removal of front side turret, see 8-9

Installation of front side turret.

2. Set light shielding tube inside the light illuminator.

(1)

Note

(6)

(7)

(5)
(both sides)

ASSEMBLY / Installation of UV-cut plate

II .

DSU

II .

8-17

Page

8-15 Installation of UV-cut plate

8-15-1 In case of BX

1. Put UV-cut plate underneath the front side mirror turret as illustrated.

8-15-2 In case of IX

1. Loosen fixing knob (1) for UV-cut plate.

2. Put the UV-cut plate in receptacle of lens barrel of microscope frame as

illustrated.

3. Turn fixing knob to fix firmly.

8-16 Installation of filter or shutter (In case of U-FWR or IX2-SHA)

1. Loosen fixing screws (1) that fix lamp house mount adapter at two

places and remove the lamp house mount adapter.

2. Insert filter or shutter (2) that you are going to use into mount (3) and

tighten fixing screws (1) at two places.

3. Insert lamp house mount adapter into filter wheel or shutter (2) that you

are going to use and then, tighten fixing screws (4) at two places.

Installation of LAMBDA 10 and Ludl filter is the same as

described above.

(2)(3)

(4)(1)

TIP

(1)

ASSEMBLY / Filter IN/OUT inside motorized filter wheel

II .

DSU

II .

8-18

Page

8-17 Filter IN/OUT inside motorized filter wheel

At ex-factory, filters are attached as shown in the following table. When you wish to

change the setting, follow to the following procedures.

No.1 No.2 No.3 No.4 No.5 No.6

Empty ND25 ND12 ND6 ND3 ND1.5

In case of installation or removal of filter, you must turn power

of control box. In addition, you must remove cable from

control box.

1. Remove lamp house and filter unit of option.

2. Loosen fixing screws (1) of mount for illuminator at two places, using

the Allen screwdriver and remove filter wheel (2).

3. Loosen fixing screws (3) of filter wheel cover at four places, using the

Allen screwdriver and remove the cover.

Filter is of 32mm diameter (25mm diameter, use conversion

adapter* ) and thickness should be up to 6mm. Direction of

presser ring is shown in the following table by the thickness.

* Conversion adapter for 25mm diamter is available at option. When

you need it, contact local sales office. (AD6921 and AB9169)

Thickness of filter Direction of presser ring Remarks

• 4mm or more, 6mm or less

• U-FWCO1.25X

• U-FW32PO

• Insert U-FWCO1.25X with 29.6mm diameter

side down

• Insert U-FW32PO into No.1 hole for sure.

(Alignment pin and slot must be matched with

each index).

• 4mm or less

• Conversion adapter
(Installation method of 25mm filter to conversion adapter is the same as described above).

Note

TIP

(2)

(3)

(1)

ASSEMBLY / Field iris diaphragm IN/OUT

II .

DSU

II .

8-19

Page

4. Insert presser ring driver (4) into slots (5) of presser ring and turn it in

clockwise direction to fix the filter (6).

When you attach filter of 25mm diameter to conversion adapter,

use small side of presser ring driver (4).

Be careful when you attach conversion adapter as the portion to

insert filter is slanted by 5°.

Arrow mark is provided on side of barrier Filter and excitation

filter.

Barrier Filter: Insertion must be matched with arrow.

Excitation filter: Insertion must be made in reverse with arrow.

5. When you finished installation of all filters required, return the filter wheel

cover to original position and fix it by tightening four fixing screws (3).

6. Insert filter wheel (2) into illuminator and tighten fixing screws (1) of mount

at two places, using the Allen screwdriver.

7. Attach lamp house and optional filter unit to original position respectively.

8-18 Field iris diaphragm IN/OUT

1. (Removal). Loosen fixing screw (1) located at the bottom of aperture

unit, using the Allen screwdriver.

2. Hold aperture unit (2) and pull it horizontally.

3. (Installation). Reverse procedure 1 and 2.

When you tighten fixing screw (1), tighten it lightly.

Do not tighten it too hard.

(4)

(5)

(6)

TIP

TIP

(1)

(2)

Note

ASSEMBLY / Installation of camera adapter and filter

II .

DSU

II .

8-20

Page

8-19 Installation of camera adapter and filter

8-19-1 In case of DSU C-mount adapter

1. Loosen fixing screw (1), using the Allen screwdriver.

2. Insert C-mount adapter (3) into disk box (2).

3. Tighten fixing screw (1), using the Allen screwdriver.

8-19-2 C-mount adapter for DSULAMBDA10 / C-mount adapter for Ludl

In case of LAMBDA10

1. Screw in the upper adapter and lower adapter by turning them into

threaded holes (1) and (2) of C-mount for filter wheel and fix them

firmly as illustrated.

2. Align filter wheel with red index (3) of port as illustrated and tighten

screws (4) at three places, using tool that comes with the system as

accessory.

3. Attach filter wheel to mirror holder in a way that red index (3) of port

and red index (5) of mirror holder are aligned each other.

4. Tighten fixing screw (6), using the Allen screwdriver.

(1)

(2)(3)

(1)

(2)

(4)

(3)

(Red index)

(5)

(Red index)

(6)

ASSEMBLY / Installation of camera adapter and filter

II .

DSU

II .

8-21

Page

5. Loosen fixing screw (7) of disk box, using the Allen screwdriver.

6. Insert C-mount adapter that is attached with filter wheel into mount

(8) of disk box.

7. Tighten fixing screw (7), using the Allen screwdriver.

In case of Ludl

1. Open lid of filter wheel and remove adapter that is attached from the

beginning, using Philips type + screwdriver.

2. Align direction with red index (9) of port as illustrated and fasten four

screws (10) that come as accessories from within, using Philips

type + screwdriver.

3. Assemble C-mount adapter as in the procedure 3 and 4 of

LAMBDA10.

4. Loosen fixing screw (11) of disk box, using the Allen screwdriver.

5. Insert C-mount adapter that is attached with filter wheel into mount

(12) of disk box.

6. Tighten fixing screw (11), using the Allen screwdriver.

(8) (7)

(9)

(10)

(11)(12)

ASSEMBLY / Installation of camera

II .

DSU

II .

8-22

Page

8-20 Installation of camera

1. Loosen fixing screw (1) of port, using the Allen screwdriver that comes

as accessory for microscope frame and remove C-mount adapter (2)

inside the port.

2. Screw in and fix the C-mount adapter(2) that was removed to C-mount

section (3) of camera firmly.

3. Insert C-mount adapter that is attached with camera into port (4)

gently.

4. Tighten fixing screw (1) at least one place, using the Allen

screwdriver.

(1)

(2)

(3)

(4)

ASSEMBLY / DIP switch settings on control box (BX61, BX62 and BX61W1 only)

II .

DSU

II .

8-23

Page

8-21 DIP switch settings on control box (BX61, BX62 and BX61W1 only)

DIP switch settings should be done after power of control box is

turned OFF.

When you use BX61, BX62 or BX61W1, you must change DIP switch settings on control

box. Set in accordance with the following procedures.

1. Peel off the seal in front of SW1.

2. Change DIP switch 7 to ON.

3. Attach front seal to SW1.

8-22 Installation of motor drive board

You must install motor drive board after you turn power of control

box to OFF.

1. Loosen four fixing knobs for two option slot covers at left side of back panel

of control box (1) and remove covers and knobs.

2. Aligning direction of connector inside control box with connector (2), insert

the board along with board rails.

Push the board until connectors of motor drive board are inserted into

connectors of mother board inside the control box.

3. Using knobs you removed, fix motor drive board (2).

Likewise, attach the other cover (3).

Slot cover section must overlap. Attach cover from left side,

seen from back side.

Keep one piece of slot cover that remained with care as it

may be required for motor drive board when it is removed.

Note

(1)

(2)

(3)

Note

Note

Note

ASSEMBLY / Z board setting and installation

II .

DSU

II .

8-24

Page

8-23 Z board setting and installation

8-23-1 In case of BX61 and BX62

For Z board, use type U-ZPCB(T2) for sure.

In case that U-ZPCB(T2) or subsequent is not used, the following

troubles may occur even if correct setting was done.

●

Rotation of focusing handle and actual objective lens movement would

be reversed.

●

Focusing control may not be able to initialize, depending upon the

position. (Microscope does not boot up).

All DIP switches on Z board, S1, S2 and S3 are turned to OFF at ex-factory.

Setting change of ONBOARD DIP SW

Use DIP SW set to OFF except the following DIP SW.

Turn DIP SW that is to be changed only to ON .

S2 NO.4

• Interchanges F/C change button and Stage retreat button located at left

side of BX61/BX62 to Stage DOWN button and Stage UP button.

S3

NO.1

• Automatic initializing function is turned to OFF and default remote state

is set.

S3

NO.4

• Stroke of focusing control is changed from 15mm to 25mm.

Z board installation

You must install Z board after you turn power of control box

to OFF.

1. Loosen fixing knobs (6 pieces) for two slot covers at back side of control

box (1) and remove covers and knobs.

2. Aligning direction of connector of control box and connector of Z board

(2), insert board along with board rails. Push in until connectors are

firmly connected.

Note

(1)

(2)

(3)

TIP

Note

Note

ASSEMBLY / Z board setting and installation

II .

DSU

II .

8-25

Page

3. Using knobs you removed, fix Z board (2). Likewise, attach the other

cover (3).

Keep one piece of slot cover that remained with care as it

may be required for Z board when it is removed.

8-23-2 In case of BX61W1

For Z board, use type U-ZPCB(T2) for sure.

In case that U-ZPCB(T2) or subsequent is not used, the following

troubles may occur even if correct setting was done.

●

Rotation of focusing handle and actual objective lens movement would

be reversed.

●

Focusing control may not be able to initialize, depending upon the

position. (Microscope does not boot up).

All DIP switches on Z board, S1, S2 and S3 are turned to OFF at ex-factory.

Setting change of ONBOARD DIP SW

Use DIP SW set to OFF except the following DIP SW.

Turn DIP SW that is to be changed only to ON .

S2 Changes NO.2 and NO.3 to ON.

S3

Changes NO.2, NO.4 and NO.5 to ON.

If DIP switches are not correctly set, objective lens comes

down and hits the specimen.

Note

Note

TIP

Note

Note

ASSEMBLY / Z board setting and installation

II .

DSU

II .

8-26

Page

Z board installation

You must install Z board after you turn power of control box

to OFF.

1. Loosen fixing knobs (6 pieces) for two slot covers at back side of control

box (1) and remove covers and knobs.

2. Aligning direction of connector of control box and connector of Z board

(2), insert board along with board rails.

Push in until connectors are firmly connected.

3. Using knobs you removed, fix Z board (2). Likewise, attach the other

cover (3).

Keep one piece of slot cover that remained with care as it

may be required for Z board when it is removed.

8-23-3 In case of IX81

For Z board, use type U-ZPCB(T2) for sure.

In case that U-ZPCB(T2) or subsequent is not used, the following

troubles may occur even if correct setting was done.

●

Rotation of focusing handle and actual objective lens movement would

be reversed.

●

Focusing control may not be able to initialize, depending upon the

position. (Microscope does not boot up).

All DIP switches on Z board, S1, S2 and S3 are turned to OFF at ex-factory.

Note

Note

Note

TIP

(1)

(2)

(3)

ASSEMBLY / Z board setting and installation

II .

DSU

II .

8-27

Page

Setting change of ONBOARD DIP SW

Use DIP SW set to OFF except the following DIP SW.

Turn DIP SW that is to be changed only to ON .

S3 Changes NO.4 and NO.5 to ON.

If DIP switches are not correctly set, objective lens comes

down and hits the specimen.

Z board installation

You must install Z board after you turn power of control box

to OFF.

1. Loosen fixing knobs (6 pieces) for two slot covers at back side of control

box (1) and remove covers and knobs.

2. Aligning direction of connector of control box and connector of Z board

(2), insert board along with board rails.

Push in until connectors are firmly connected.

3. Using knobs you removed, fix Z board (2). Likewise, attach the other

cover (3).

Keep one piece of slot cover that remained with care as it

may be required for Z board when it is removed.

(1)

(2)

(3)

Note

Note

Note

Note

OLYMPUS CORPORATION

2-43-2,Hatagaya, Shibuya-ku, Tokyo, Japan

OLYMPUS OPTICAL CO.(EUROPA) GMBH.

Postfach 10 49 08, 20034, Hamburg, Germany

OLYMPUS AMERICA INC.

2 Corporate Center Drive, Melville, NY 11747-3157, U.S.A.

OLYMPUS SINGAPORE PTE LTD.

491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373

OLYMPUS OPTICAL CO. (U.K.) LTD.

2-8 Honduras Street, London EC1Y OTX, United Kingdom.

OLYMPUS AUSTRALIA PTY. LTD.

104 Ferntree Gully Road, Oakleigh, Victoria, 3166, Australia

Printed in Japan 2003 10

1.3