Olympus BX51WI User Manual [ru]
INSTRUCTIONS
BX51WI
FIXED-STAGE UPRIGHT
MICROSCOPE
This instruction manual is for the Olympus Fixed-Stage Upright Microscope Model BX51WI. To
ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of
this microscope, we recommend that you study this manual thoroughly before operating the
microscope. Retain this instruction manual in an easily accessible place near the work desk for
future reference.
A X 7 6 5 7
BX51WI
CONTENTS
Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to
assemble the microscope yourself, please read Chapter 9, “ASSEMBLY” (pages 36 to 42) carefully. For the modules
provided with instruction manuals, also read the assembly procedures in their instruction manuals.
IMPORTANT — Be sure to read this section for safe use of the equipment. —
1 MODULE NOMENCLATURE
2 CONTROLS
TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE
3
4 USING THE CONTROLS
4-1 Microscope Base, Power Supply Unit (TH4)................................................................................................ 10
1 Controlling the Light Intensity (TH4) 2 Using the Filter Turret
4-2 Focusing Block........................................................................................................................................................................................ 11
1 Using the Pre-focusing Lever 2 Using the Fine Adjustment Fast-Feed Knob
3 Using the Frost Switching Lever
4 Adjusting the Coarse Adjustment Knob Rotation Tension
4-3 Stage (IX-SVL2) ............................................................................................................................................................................. 12, 13
1 Placing the Specimen 2 Moving the Specimen
3 Setting the Grounding 4
5 Using the Light Shield Sheet 6 Lowering the Stage Height
4-4 Revolving Nosepiece .................................................................................................................................................................... 13
1 Switching the Objectives (U-SLRE, WI-SRE3)
4-5 Observation Tube ..................................................................................................................................................................... 14, 15
1 Adjusting the Interpupillary Distance 2 Adjusting the Diopter
3 Using the Eye Shades 4 Using Eyepiece Micrometer Disks
5 Selecting the Light Path of Trinocular Tube
4-6 Condenser .......................................................................................................................................................................................... 16, 17
1 Centering the Condenser (Field Iris Diaphragm, Aperture Iris Diaphragm)
2 Oblique Illumination (WI-OBCD)
4-7 Immersion Objectives .................................................................................................................................................................. 18
1 Using Water Immersion Objectives (Water Immersion Cap for XL Objectives XL-CAP)
Adjusting the X-Axis/Y-Axis Knob Rotation Tension
1-2
3
4-7
8, 9
10-18
5 OTHER OBSERVATION METHODS
5-1 Differential Interference Contrast Observation............................................................................... 19-23
1 Attaching the Analyzer 2 Attaching the Polarizer
3 Attaching the DIC Prisms (for Revolving Nosepiece)
4 Attaching the DIC Prisms (for Condenser)
5 Adjusting the Polarizer Position (except the U-UCD8)
6 Observation Method
5-2 Reflected Light Fluorescence Observation................................................................................................... 24
5-3
Infrared Light (IR)/Differential Interference Contrast (DIC) Observation
1 Introduction 2 Attaching the IR Modules
3 DIC Observation Using IR
5-4 Macro Reflected Light Fluorescence Observation ................................................................. 28-30
1 Introduction 2 Attaching the Modules
3 Filter Characteristics of Fluorescence Mirror Units
4 Fabricating Optional Mirror Unit
19-30
............... 24-27
6 TROUBLESHOOTING GUIDE
7 SPECIFICATIONS
8 OPTICAL CHARACTERISTICS
9
ASSEMBLY
PROPER SELECTION OF THE POWER SUPPLY CORD ...................................................................
—
See this section for the replacement of the light bulb. —
10 LAMP HOUSING INSPECTION SHEET
This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic medical devices. CE marking means the conformity to the directive.
NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case
the user will be required to correct the interference at his own expense.
31-33
34
35
36-42
43, 44
45
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user’s authority to operate the equipment.
IMPORTANT
This microscope employs a UIS2 (UIS) (Universal Infinity System) optical design, and should be used only
with UIS2 (UIS) eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed
for the BX series are also usable. For details, please consult Olympus or the latest catalogues.)
To obtain comprehensive understanding on the operating procedures, please also read the separately
provided instruction manuals.
Instruction manual
BX51WI
TH4
BX-URA2/BX-RFA
WI-DPMC
WI-XYM/XYS
WI-SSNP
Explanation of transmitted brightfield observation, differential
interference contrast observation and infrared observation
Explanation of the external halogen bulb power supply unit
Explanation of reflected light fluorescence observation
Explanation of the variable-magnification dual-port observation tube
Explanation of the XY mover/bridge stage
Explanation of the swinging-sliding revolving nosepiece
SAFETY PRECAUTIONS
Contents
BX51WI
1. After the equipment has been used in an observation of a specimen
²
@
Fig. 1
that is accompanied with a potential of infection, clean the parts
coming in contact with the specimen to prevent infection.
· Moving this product is accompanied with the risk of dropping the
specimen. Be sure to remove the specimen before moving this
product.
· In case the specimen is damaged by erroneous operation, promptly
take the infection prevention measures.
2. Culture liquid or water spilt on the stage, condenser or microscope may
damage the equipment. Immediately wipe the liquid or water off if it is
spilt on them.
3. When moving the microscope, disconnect the reflected light illuminator,
observation tube and transmitted light lamp housing and carefully carry
the microscope by the base (front edge) @ and the grasping part on the
rear of the arm ² as shown in Fig. 1. (Weight: approx. 15 kg.)
Also be careful against slipping of hands during carrying.
# Damage to the microscope will occur if you grasp it by other parts
including the stage, coarse/fine adjustment knob, etc.
Safety Symbols
The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment
in the safest possible manner.
Symbol Explanation
Indicates that the surface becomes hot, and should not be touched with bare hands.
Before use, carefully read the instruction manual. Improper use could result in personal injury to
the user and/or damage to the equipment.
1 Getting Ready
1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.
2. The U-SWTR-3 super-widefield observation tube (FN 26.5) cannot be used with the BX51WI microscope.
3. The BX51WI microscope can be used with an intermediate attachment (such as a BX-URA2 or BX-RFA reflected light
illuminator, U-ECA or U-CA magnification changer, etc.).
Two intermediate attachments can be used only in the following conditions:
· The U-CA or U-ECA magnification changer or U-FWO filter wheel can be mounted as the second attachment.
· When a TV adapter with 1X or higher power is used, 2/3-inch CCD TV observation is possible.
· The peripheral areas of the field of view may be obscured or cut off in binocular observation using the U-TR30-2, U-ETR
or U-TR30IR (FN 22) super-widefield observation tube.
1
4. In IR (infrared) observation, the U-CA or U-ECA magnification changer can be used only when the U-ETR3 or U-TR30IR
observation is used.
5. In photomicrography with visible light, correct exposure may be impossible if the microscope is set for IR observation.
Be sure to engage the provided IR cut filter (light blue) before photomicrography.
6. When the XLUMPlanFl20XW objective is used, only the U-TV1X-2, U-TVCAC, U-PMTVC2XIR or U-PMTVC4XIR TV adapter
can be used.
7. Do not attempt to remove or loosen the click springs and screws. Otherwise, Olympus can no longer warrant the
performance of the microscope.
The clicking force of the revolving nosepiece has been set weak in order to reduce vibrations during objective switching.
To reproduce the correct click position, switch the objectives gently by operating the lever.
8. Caution for use of the U-ETR3 upright trinocular tube:
When the aperture stop of the condenser is reduced using a reflected light fluorescence illuminator and the LUMPlanFl60XW
objective, part of the observed field of view may be obscured slightly. This is due to the reduction of the light intensity in
the field of view due to the narrow aperture and is not due to a defective optical adjustment of the microscope.
This phenomenon does not affect the photomicrography or TV camera light path.
2 Maintenance and Storage
1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and
wipe gently using a piece of cleaning paper (or clean gauze).
If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute
alcohol.
Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks –– for example, electrical
equipment that is being switched on or off.
Also remember to always use it only in a well-ventilated room.
2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean
them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
3. Never attempt to disassemble any part of the microscope.
4. When not using the microscope, make sure to set the main switch to “ ” (OFF), confirm that the lamp housing is cool
enough and cover the microscope with the provided dust cover.
5. When disposing of this unit, check the regulations and rules of your local government and be sure to observe them.
3 Warning Indication
A warning sticker is attached to a part where special precaution is required when handling and using the system. Always
heed the warning.
Warning indication
position
Lamp housing (U-LH100-3/U-LH100IR)
(Warning against high temperature)
4 Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,
the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
The following symbols are used to set off text in this instruction manual.
: Indicates that failure to follow the instructions in the warning could result in bodily harm to the
user and/or damage to equipment (including objects in the vicinity of the equipment).
# : Indicates that failure to follow the instructions could result in damage to equipment.
} : Indicates commentary (for ease of operation and maintenance).
Intended use
5
This instrument has been designed to be used to observe magnified images of specimens in routine and research
applications.
Do not use this instrument for any purpose other than its intended use.
2
BX51WI
MODULE NOMENCLATURE
}The modules shown below are only the representative modules. As there are other modules which can be combined with
the microscope but are not shown below, please also refer to the latest Olympus catalogues or your dealer.
Transmitted Arm BX-ARM
or Reflected Light Fluorescence Illuminator
· BX-URA2
· BX-RFA
Trinocular Observation Tube
· U-TR30-2 (FN 22)
· U-TR30IR (FN 22)
· U-ETR3 (FN 22)
Revolving Nosepiece
· WI-SRE3
· WI-SNPXLU2
· U-SLRE
Revolving Arm
WI-NPA
Cross Stage
IX-SVL2
or commercially
available bridge
stage
Fixed-Stage Adapter
WI-FSH
Reflected Light Mercury Lamp Housing
· U-LH100HG
· U-LH100HGAPO
Microscope Frame BX51WIF
Power Supply Unit
U-RFL-T
Transmitted Light Lamp Housing
· U-LH100-3
· U-LH100IR
Condenser
· WI-UCD
· WI-DICD
· WI-OBCD
· U-UCD8
· U-SC3
· U-AAC
· U-AC2
Power Supply Unit (for Halogen Bulb)
TH4
Hand Switch TH4-HS
Objective
· MPLN5X
· UMPlanFLN10XW/20XW
· LUMPlanFLN40XW/60XW
· LUMFLN60XW
· LUMPlanFl100XW
· XLUMPlanFI20XW
(exclusively for use with the WI-SNPXLU2)
· XLFluor2X/340
(exclusively for use with the U-SLRE)
· XLFluor4X/340
(exclusively for use with the U-SLRE)
3
CONTROLS
}If you have not yet assembled the microscope, read Chapter 9, “ASSEMBLY” (pages 36 to 42).
Light path selector knob (Page 15)
Interpupillary distance
scale (Page 14)
Diopter adjustment
ring (Page 14)
Reflected light
fluorescence illuminator
(See the separately
provided instruction
manual.)
Stage center plate
Reflected light mercury lamp housing
(See the separately provided instruction
manual.)
Power supply unit
(See the separately
provided instruction
manual.)
Y-axis knob (Page 12)
X-axis knob (Page 12)
Rubber cap for fine
adjustment knob
(Page 11)
Centering knob
(Accommodation position)
Pre-focusing lever (Page 11)
Coarse adjustment knob (Page 11)
Power supply unit
(See the separately provided
instruction manual.)
Coarse adjustment knob (Page 11)
Coarse adjustment knob rotation tension adjustment ring (Page 11)
Fine adjustment knob (Page 11)
4
}The descriptions on the filter turret, revolving nosepiece, condenser, etc. will be given in the subsequent pages.
BX51WI
Filter Turret
Polarizer positions (Page 19)*
Accepts the 32PO or 32POIR.
Indication label
attaching frame
Filter positions
Accepts 32 mm filters
with thickness of no
more than 6 mm.
Polarizer clamping knob (Page 19)
Polarizer push ring (Page 10)
Made of white plastic
Polarizer rotating dials
(Page 23)
Slider insertion/removal
stopper (Page 10)
IR filter insertion position
Accepts the 32BP775, 32IR900 or a 32 mm
filter with thickness of no more than 5.3 mm.
* Accepts the combination of a
polarizer push ring and a 32 mm
filter. The thickness should be no
more than 6 mm.
Filter frame reinforcing ring
Filter slider (Page 10)
Revolving Arm WI-NPA
# Note that the revolving arm can be mounted only before the reflected light illuminator is mounted or before the
transmitted light arm and IX-SVL2 stage are mounted (page 38).
}This revolving arm accepts the U-SLRE, WI-SNPXLU2 or WI-SRE3 revolving nosepiece.
(Page 38)
Light shield tube retaining screw
Revolving nosepiece mount dovetail
Revolving nosepiece clamping screw
Sliding Revolving Nosepiece U-SLRE
Switching lever
XLFluor objective mount screw
M 34 mm, pitch 1 mm
Single-Position Revolving Nosepiece XLU
WI-SNPXLU2
DIC mount hole
UIS2 (UIS) objective
mount screw
Grounding screw hole (M3)
Microscope frame mount dovetail
(Page 20)
XLU objective mount screw
M 25 mm, pitch 0.75 mm
UIS2 (UIS) objective mount screw
RMS Adapter WI-RMSAD
5
Swinging Revolving Nosepiece WI-SRE3
Swing lever
Confocality correction washers
(Kinds of thickness: 10, 30 and
50 μm; three washers per kind)
Centering screw
UIS2 (UIS) objective mount
screws
Long-WD Universal Condenser WI-UCD
Turret (4 positions)
Quarter-wave plate rotation
ring (Page 23)
DIC mount hole (Page 20)
Quarter-wave plate clamping
knob
Optical element index
mount (Page 21)
Centering knob
Aperture iris diaphragm lever (Page 17)
DIC prism replacement cover
Long-WD DIC Condenser WI-DICD
Aperture iris diaphragm
lever (Page 17)
Quarter-wave plate
clamping knob
6
DIC prism (large) mount position
DIC prism (small) adapter
Quarter-wave plate rotation
ring (Page 23)
BX51WI
Differential Interference Contrast Prisms (For Condenser)
}The WI-UCD condenser accepts two large and two small DIC prisms while the WI-DICD condenser accepts one large or
small DIC prism.
When selecting the brightfield (BF) light path using the WI-UCD, leave one DIC prism (large) mount position empty.
Positioning indices
· WI-DIC10HR
· WI-DIC20HR
Index
Long-WD Oblique Condenser WI-OBCD
Aperture iris diaphragm lever (Page 17)
High-Resolution DIC Prism A WI-DICTHRA2
DIC Prism WI-DICT2
· WI-DIC40HR
· WI-DIC60HR
· U-LDPXLU20HR
Index
Oblique iris insertion/removal knob (Page 17)
}This prism can be mounted in the DIC prism position
of the WI-SNPXLU2 or WI-SRE3.
Positioning pin
Applicable condensers
WI-DICTHRA2: WI-UCD, WI-DICD
WI-DICT2: U-UCD8
Condensers and Applicable Objective Magnifications
Condenser
WI-UCD
WI-DICD
WI-OBCD
U-UCD8
U-SC3
U-AAC 10X or more
U-AC2 5X or more
Applicable Objective Magnification
5X or more
2X or more
7
TRANSMITTED LIGHT BRIGHTFIELD
OBSERVATION PROCEDURE
}The following flow shows the operating procedure for the transmitted light brightfield observation which is the basic obser-
vation method of this microscope. The operating procedures for DIC observation, fluorescence DIC observation and IR DIC
observation will be described separately in Chapter 5, “OTHER OBSERVATION METHODS” on page 19.
Set the main switch to “ I ” (ON).
Disengage the filter from the light
path.
Select the binocular light path.
Place the specimen on the stage.
Engage a low-magnification objective in the
light path.
Bring the specimen in focus.
Adjust the brightness.
(Controls Used)
@Main switch (P. 10)
²Lamp ON-OFF switch (P. 10)
(Only when the hand switch is used)
³Filter turret (P. 10)
|Light path selector knob (P. 15)
ƒX-axis and Y-axis knobs (P. 12)
…Swing lever (P. 13)
†Coarse/fine adjustment knobs (P. 11)
‡Light intensity control knob (P. 10)
(Page)
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
Adjust the aperture iris and field iris diaphragms.
Engage the objective to be used in the light
path and bring the specimen in focus.
Engage the required filters.
Adjust the brightness.
Start observation.
Tip for microscope operation
ŠBinocular tube (P. 14)
‰Diopter adjustment ring (P. 14)
‹Condenser height adjustment knob (P. 16)
ŒCondenser centering knob (P. 16)
™Aperture iris diaphragm lever (P. 17)
šField iris diaphragm ring (P. 16)
…Swing lever (P. 13)
†Coarse/fine adjustment knobs (P. 11)
³Filter turret (P. 10)
‡Light intensity control knob (P. 10)
8
In patch-clamp testing, switch the microscope controls cautiously and gently so that the patch
electrodes do not slip off.
‰
BX51WI
|
Š
…
ƒ
‹Œ™
†
³
š
†
†
@
²
‡
‡
Used only when the hand
switch is not connected
} Make a photocopy of the observation procedure pages and post it near your microscope.
9
USING THE CONTROLS
4-1 Microscope Base, Power Supply Unit (TH4)
ƒ
³
|
²
@
Fig. 2
Fig. 3
…
1 Controlling the Light Intensity (TH4)
}See the separately provided instruction manual for details.
1. Make sure that the light intensity control knob @ is set to MIN (minimum
voltage), then set the main switch ² to “ I ” (ON). (The POWER LED ³
should light up.)
2. Turn the knob @ clockwise toward MAX (maximum voltage) to increase
the intensity and brightness.
}The marking indicates the position where the optimum daylight for
color photography is obtained when the LBD filter is engaged in the light
path.
Operation Using the Hand Switch
}When the hand switch is connected (when the REMOTE LED | is lit), the
light intensity control knob @ is defeated and only the light intensity
control knob ƒ of the hand switch can be used.
The hand switch is provided with double-side adhesive tape so that it
can be attached onto a convenient position for operation.
1. After setting the main switch ² to “ I ” (ON), press the lamp ON-OFF switch
… to ON and adjust the brightness with the intensity control knob ƒ.
2. To turn the lamp OFF, press the lap ON/OFF switch … again to OFF.
#The lighting of the REMOTE LED | indicates that the hand switch is
standing by. The hand switch consumes a power of about 2.5 W
when it stands by.
When the system is not to be used for a lone period, be sure to set
the main switch ² to “ ” (OFF).
(Figs. 2 & 3)
³
ƒ
@
Fig. 4
²
|
2 Using the Filter Turret
}Filters with a diameter of 32 mm can be inserted in positions @ to |.
1. Filter positions @ and ² are rotatable. When the 32PO polarizer or 32POIR
polarizer is placed in either position, the polarizer or filter can be fixed by
using the push ring (made of white plastic).
}When filter position @ is engaged in the light path, the rotation fixing
knob ƒ comes at the front where the operation is easy.
2. Filter position ³ accepts any type of 32 mm filter.
#When using two filters together, the thickness of the lower filter should
be no more than 2 mm. Otherwise, the upper filter may drop during
rotation.
3. Filter position | accepts the 32BP775 or 32IR900 filter. As the filter cannot be inserted unless the filter slider is removed, remove it by releasing
the insertion/removal stopper below the slider and loosening the slider
clamping screw using the provided Allen screwdriver.
(Fig. 4)
10
4-2 Focusing Block
BX51WI
@
²
@
Fig. 5
Fig. 6
²
³
1 Using the Pre-focusing Lever
}The pre-focusing lever prevents collision between the specimen and
objective and simplifies the focusing operation. After bringing the
specimen into approximate focus with the coarse adjustment knob, turn
the pre-focusing lever @ in the direction of the arrow to lock it. Hereafter,
the lower limit of the coarse adjustment will be limited at the position
where the lever is locked. When bringing a specimen in focus, approximate
focus can be obtained by simply lowering the coarse adjustment to the
stopper position so all you have to do more is control the fine adjustment
knob.
}The up/down movement using the fine adjustment knob is not limited.
# When the pre-focusing lever is locked, the coarse adjustment stroke
is limited by the mechanism and it cannot reach the previous upper
limit. If you want to control the coarse adjustment knob to the previous
upper limit, unlock the pre-focusing lever.
2 Using the Fine Adjustment Fast-Feed Knob
}Fine focusing is usually possible while the rubber cap @ is attached.
However, when it is desirable to allow the fine adjustment knob to vary
focusing by a large amount, though this is not be as large as with the
coarse adjustment knob, the rubber cap can be removed and the provided fine adjustment fast-feed knob attached in place.
}If you remove the knob by loosening the screw clamping the fine adjust-
ment knob ³ from the opposite side, the fine adjustment can be controlled using the tip or thick of your finger.
(Fig. 5)
(Fig. 6)
@
Fig. 7
3 Using the Frost Switching Lever
}Low observation light can be brightened by turning the frost switching
lever @ which controls the built-in frost filter, in the direction of the arrow.
However, although the brightness is increased, irregularity in lighting may
also increase.
4
Adjusting the Coarse Adjustment Knob Rotation Tension
# Do not adjust the coarse adjustment knob rotation tension adjust-
ment ring (² in Fig. 5) because the belt interlocking of the ring with
the coarse adjustment knob on the front has been adjusted at the
factory. If the tension is varied, the accuracy of the pre-focusing lever
will deteriorate.
(Fig. 7)
(Fig. 5)
11
4-3 Stage (IX-SVL2)
@
²
Fig. 8
Fig. 9
1 Placing the Specimen
1. Place the specimen on the center of the stage.
}The optional stage center plate (IX-CP50) makes it possible to observe a
wide range of a big petri dish, etc. (Central hole diameter: 50 mm)
2 Moving the Specimen
1. The specimen can be moved by turning the X-axis knob @ and Y-axis
knob ².
The movement strokes are 50 mm (X-axis) x 43 mm (Y-axis).
(Fig. 8)
(Fig. 9)
Fig. 10
Fig. 11
@
@
²
3 Setting the Grounding
}In case of electrical physiological experiment, etc., the specimen can be
grounded from the stage.
Prepare a grounding wire @ and M4 screw ² and attach grounding as
shown in Fig. 10.
# The screw hole may sometimes be stuck by paint, etc. In such a case,
screw in the M4 screw a few times to expose the metallic thread
inside the screw hole and improve the contact before attaching the
grounding wire firmly.
4
Adjusting the X-Axis/Y-Axis Knob Rotation Tension
}The rotation tension of the X-axis and Y-axis knobs can be adjusted
independently.
1. Loosen the 2 set screws @ of a knob using the provided Allen wrench,
hold the stage so that it will not move, then turn the knob to adjust the
tension. Turning it in the direction of the arrow increases the tension and
turning in the opposite direction decreases the tension.
2. After adjustment, tighten the set screws firmly.
# If the tension of a knob is too heavy or too light, skipping or returning of
image may occur during the stage movement.
(Fig. 10)
(Fig. 11)
12
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