Olympus BX46 User manual

INSTRUCTIONS

BX46

CLINICAL MICROSCOPE

This instruction manual is for the Olympus Clinical Microscope Model BX46.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use
of this microscope, we recommend that you study this manual thoroughly before operating the
microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.

A X 7 8 5 3

In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equipment,
this symbol indicates that the product must not be disposed of as unsorted municipal waste, but
should be collected separately.
Refer to your local Olympus distributor in EU for return and/or collection systems available in your
country.

NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,

pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.

Operation of this equipment in a residential area is likely to cause harmful interference in which case

the user will be required to correct the interference at his own expense.

FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance

could void the user's authority to operate the equipment.

BX46

CONTENTS

Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble
the microscope yourself, please read section 10, “ASSEMBLY" (pages 31 to 34) carefully.

IMPORTANT -- Be sure to read this section for safe use of the equipment. --

1 MODULE NOMENCLATURE

2 CONTROLS

3 FLOW OF OBSERVATION

4 SIMPLIFIED OBSERVATION PROCEDURE

4-1 Basic Operation (Until Observation of Specimen)

4-2 Microscope Adjustments (How to Improve the Observed Image)

1 Adjusting the Interpupillary
Distance

3 Adjusting the Centering

.................................................................................

..................................

2 Adjusting the Diopter

11

4 Adjusting the Contrast

12

........................................................................

........................

..........................................

......................................

5 USING THE CONTROLS

5-1 Base

.................................................................................................................................................................................................................

1-3

4

5-7

8,9

10-12

10,11

11,12

11

12

13-23

13,14

1 Adjusting the Brightness

3 Using the Filters

5-2 Focusing Block

1 Focusing Controls

3 Replacing the Fine Adjustment
Knob

5 Pre-focusing Lever

5-3 Stage

1 Placing the Specimen

3 Rotating the Stage

............................................................................................

................................................................................................................................................................................................................

5-4 Observation Tube

1 Adjusting the Diopter

3

Mounting the Eyepiece Micrometer

Disk
5 Adjusting the Tilt

............................................................................................

..........................................................

................................................................................................................................................................................

..................................................

..................................................

.................................................

....................................................................................................................................................................

.....................................................

...............................

.......................................

..........................................

2 Setting the LIM Brightness

13
14

2 Adjusting the Focus

15

4 Adjusting the Coarse Adjustment
Knob Tension

16

.............................................

..................................................................

16

2 Adjusting the X- and Y-Axis Knob

17

Tension

...................................................................................

18

2 Using the Eye Shades

19

4 Selecting the Light Path of the
Trinocular Tube

20

6 Using Eyepieces Incorporating a

21

Micrometer

.........................................................

......................................................................

.......................................

..........................

15,16

17,1 8

19-22

13

15

16

18

19

20

22

5-5 Condenser

...................................................................................................................................................................................................

5-6 Immersion Objectives

.................................................................................................................................................................

22

23

6 CAMERA RECORDING

24

7 TROUBLESHOOTING GUIDE

8 SPECIFICATIONS

9 OPTICAL CHARACTERISTICS

(UIS2 Series)

10 ASSEMBLY -- See this section for the replacement of the light bulb. --

11 HALOGEN LAMP SOCKET INSPECTION SHEET

n

PROPER SELECTION OF THE POWER SUPPLY CORD

..........................................................

25-27

28,29

30

31-34

35

36,37

IMPORTANT

BX46

SAFETY PRECAUTIONS

(Fig. 1)

1. If potentially infectious samples may be observed, use protective gloves or
other protective means to prevent the skin from contacting with samples
directly.

After observation, be sure to clean the portion contacted with samples.

· Moving this product is accompanied with the risk of dropping the

samples. Be sure to remove the samples before moving this product.

· In case the samples is damaged by erroneous operation, promptly

take the infection prevention measures.

· Follow the procedures described in Chapter “Getting Ready” (see on

page 2) prior to using the accessories of this product. Otherwise, the
stability of the device will be lost and the dropped samples will cause
the possibility of infection.

· When you maintain the device which may have contacted with

potentially infectious reagents, be sure to wear the protectors such as
gloves, or clean the device prior to operation.

· Before disposing of device contacted with potentially infectious samples,

be sure to follow the regulations and rules of your local government.

2. Install the microscope on a sturdy, level table or bench.

3. Be careful not to tilt the microscope too much. Otherwise, the mobile
parts such as the light path selector knob may move in unintended
directions.

4. Always use the power cord provided by Olympus. If no power cord is
provided, please select the proper power cord by referring to the section
“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of
this instruction manual.

If the proper power cord is not used, product safety performance cannot

be warranted.

5. Always ensure that the grounding terminal of the microscope and that of
the wall outlet are properly connected. If the equipment is not grounded,
Olympus can no longer warrant the electrical safety performance of the
equipment.

1

Fig. 1

Operation Using the LED Lamp

Safety Note on LED (Light Emitting Diode)

The LED incorporated in the LED lamp housing is a class 1 LED
product. The light emitted by LED is basically safe, but do not look at
the illumination light directly for an extended period to prevent your eye
from being injured

CLASS 1 LED PRODUCT

Operation Using the Halogen Bulb

1. To avoid potential shock hazards and burns when replacing the halogen
bulb, set the main switch of the TL4 power supply unit to “
allow the lamp socket @ and bulb to cool before touching them.

Designated bulb 6V30WHAL (PHILIPS 5761)

CAUTION

Always use the designated lamp bulb. Using other bulb may

cause malfunction.

2. If the lamp cable or power cord comes in contact with the lamp socket
or its surroundings, the cable or cord may be melted by the heat, causing
an electric shock or fire hazard. To prevent this, distribute the lamp cable
and power cord at enough distances from the lamp socket.

3. The standard service life of the lamp socket is eight (8) years of use or
20,000 hours of total power ON period, whichever is the shorter period.

For details, see Inspection Sheet on page 35.

” (OFF) and

1

Restrictions in Use

When all combinations (@ to ³) below are satisfied at the same time, the microscope may fall if tilted larger than a certain

degree, comparing to other combinations. Please mount the camera port of the intermediate observation tube toward
diagonally backward 45° to prevent from falling.

@ The observation tube is either the trinocular tube, U-TTBI, U-ETBI or U-TTLBI.
² The intermediate observation tube is either U-TRUS or U-DP.
³ The camera heavier than 0.4kg is mounted on the intermediate observation tube described in ².

Safety Symbols

The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment
in the safest possible manner.

Symbol Explanation

Indicates that the surface becomes hot, and should not be touched with bare hands.

Indicates a non-specific general hazard. Follow the description given after this symbol or in
instruction manual.

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

Caution engraving/label

Caution engraving and label are placed at parts where special precaution is required when handling and using the

microscope. Always heed the cautions.

Caution engraving
position

Caution label
position

If a caution engraving or label is dirty or peeled off, contact Olympus for the replacement or other inquiry.

Halogen lamp socket
[Caution against high temperature]

Rear panel
[Caution against high temperature]

1 Getting Ready

1. A microscope is a precision instrument. Handle it with care and avoid
subjecting it to sudden or severe impact. Also note that the microscope
does not have a waterproof construction.

2. Do not use the microscope where it is subjected to direct sunlight, high
temperature and humidity, dust or vibrations. (For the operating conditions,
refer to Chapter 8, “SPECIFICATIONS”.)

3. When moving the microscope, remove the observation and specimen
and carefully carry the microscope by holding the grasping part on the
rear of the arm and the front part of the base as shown in Fig. 2.

CAUTION

Fig. 2

To prevent damage to the microscope, never hold it by the stage

or coarse/fine adjustment knob.

· Even when moving the microscope along the desktop surface, be sure

to remove the specimen in advance.

· When the microscope needs to be packaged for forwarding to a remote

location, contact Olympus.

4. When the accessories of this product are used, make sure that the system
height does not exceed 1 meter. Otherwise, the stability of the device will
be lost.

5. When an arm, illuminator, intermediate attachment, observation tube and
camera are to be attached to the upper part of the microscope frame,
make sure that their total weight does not exceed 7 kg.

(Fig. 2)

2

3

BX46

2 Maintenance and Storage

1. To clean the lenses and other glass components, simply blow dirt away using a commercially available blower and wipe
gently using a piece of cleaning paper (or clean gauze).

If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute

alcohol.

CAUTION

· Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks --- for example, electrical

equipment that is being switched on or off, which could cause ignition of a fire.

Also remember to always use it only in a well-ventilated room.

· Set the main switch to “

to maintenance.

2. Do not use organic solvents to clean the microscope components other than the glass components. To clean them, use
a lint-free, soft cloth slightly moistened with a diluted neutral detergent.

3. Do not disassemble any part of the microscope except for the parts that are specified to be disassembled in this manual,
as this could result in malfunction or reduced performance.

4. After using the microscope, set the main switch to “
halogen bulb has been used,) and keep it covered with a dust cover during storage.

5. Before disposing of this product, be sure to follow the regulations and rules of your local government.

” (OFF) and wait for the lamp housing to cool down sufficiently before proceeding

” (OFF), (wait for the lamp socket to cool down sufficiently if the

3 Caution

If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,

the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.

CAUTION

: Indicates a potentially hazardous situation which, if not avoided, may result in minor

or moderate injury or damage to the equipment or other property. It may also be used
to alert against unsafe practices.

} : Indicates commentary (for ease of operation and maintenance).

4 Intended use

This product has been designed to be used to observe magnified images of specimens in various routine work and

research applications.

Do not use this product for any purpose other than its intended use.
This product is considered a medical device in the following country: US.

This product complies with the requirements of directive 98/79/EC concerning in vitro diagnostic
medical devices. CE marking means the conformity to the directive.

Safety and EMC Precautions

This product complies with the emission and immunity requirements described in IEC61326 series.

·

· The electromagnetic environment should be evaluated prior to operation of this product. Do not use this product in
close proximity to the sources of strong electromagnetic radiation to prevent interference with the proper operation.

· Use only power cord which OLYMPUS specifies. Otherwise the safety and EMC performance of the product can not
be assured.

· Be sure to ground the product. Otherwise our intended electric safety and EMC performance of the product can not
be assured.

4

1

} The modules mentioned below show only the typical product names. As there are some products that are not mentioned but

also applicable to this microscope, check the latest catalogues or consult Olympus.

For the products marked “ * ”, also read their instruction manuals.

MODULE NOMENCLATURE

Eyepieces

· WHN10X

· WHN10X-H

Slide holder

· U-HLST-4

· U-HLS-4

Observation tube

· U-TTLBI

· U-TBI-3

· U-BI30-2

Intermediate attachment

· U-TRU

· U-CA

· U-DO3

Lamp housing

· U-LH100-3

· U-LS30ADP +
U-LS30-5 + TL4*

Stage

· U-SVRC

· U-SVRC-CY

BX46

2

2

· If you have not yet assembled the microscope, read section 10, “ASSEMBLY” (pages 31 to 34).

Diopter adjustment ring
(Page 19)

CONTROLS

Interpupillary distance
adjustment scale (Page 11)

Slide holder (Page 17)

Aperture iris diaphragm ring
(Page 12)

Filter slider insertion slot

Field iris diaphragm ring
(Page 12)

Allen screwdriver holder

Coarse adjustment knob
(Page 15)

Y-axis knob (Page 18)

X-axis knob (Page 18)

Coarse adjustment tension
adjustment ring (Page 16)

5

6

Main switch (Page 10)
I : ON

: OFF

Fine adjustment knob
(Page 15)

Detachable.

LIM setting switch (Page 13)

Pre-focusing lever
(Page 16)

Top lens swing-out
lever (Page 12)

Condenser height
adjustment knob
(Page 12)

LED brightness adjustment
knob (Page 13)

LIM ON-OFF switch (Page 13)

LIM indicator

ON : Lit (Green)
OFF : Extinguished

7

BX46

<< Modules for halogen lamp operation >>

LS30 Adapter

U-LS30ADP

Guide pin holes

Halogen Lamp Socket

U-LS30-5

Guide pins

Lamp cable

Brightness control
knob

Main switch

I : ON.

Power Supply Unit
TL4

Lamp socket connector

: OFF.

3

3

} When the LED lamp is used and the LIM is set, the LED brightness adjustment knob is defeated.
} When the halogen bulb is used, set the 32LBD filter in the filter slider and insert it into the insertion slot @.

FLOW OF OBSERVATION

(Controls Used) (Page)

Set the main switch to “ I ” (ON) and adjust
the brightness.

Select the light path (trinocular tube).

Place the specimen on the stage.

Engage the 10X objective in the light path.

Bring the specimen in focus.

Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.

LED lamp

@ Main switch
2 LED brightness
adjustment knob*

5 Light path selector knob (P. 20)

6 Slide holder (P. 17)
7 X-/Y-axis knobs (P. 18)

8 Revolving nosepiece
9 Top lens swing-out lever (P. 12)

a Coarse/fine adjustment knobs (P. 15)

b Binocular tube (P. 11)
c Diopter adjustment ring (P. 19)
d Condenser height adjustment knob (P. 12)
e Condenser centering screws (P. 12)

Halogen bulb

3 Main switch
4 Brightness

adjustment knob

(P. 13)

Adjust the aperture iris and field iris diaphragms.

Engage the objective to be used in the light
path and bring the specimen in focus.

Engage the required filters.

Adjust the brightness.

Start observation.

* The LED brightness adjustment knob is defeated when the LIM is set (see page 13).

f Aperture iris diaphragm ring (P. 12)
g Field iris diaphragm ring (P. 12)

8 Revolving nosepiece
9 Top lens swing-out lever (P. 12)

h Filter mount (P. 14)

2 LED brightness

adjustment knob*

4 Brightness
adjustment knob

(P. 13)
(P. 13)

8

9

BX46

b

c

8

(trinocular tube only)

5

1

6

f

e

2

h

g

7

4

3

9

a

d

} Copy the observation procedure pages on separate sheets and post it near your microscope.

10

4

SIMPLIFIED OBSERVATION PROCEDURE

4-1 Basic Operation (Until Observation of Specimen)

This section describes the basic operation of the microscope until the start of observation of a specimen. For the detailed

operating procedure of each control, please read the description page specified below.

Press the main switch of the microscope frame to “ I ” (ON).

1

1

Fig. 3

Rotate the knob to adjust the lamp brightness. (Details: Page 13)

2

2

Fig. 4

Rotate the knob to lower the stage. (Details: Page 16)

4

3

Fig. 5

3

Open the clamping lever of the slide holder and place the specimen

4

slide. (Details: Page 17)

11

BX46

Rotate the revolving nosepiece to engage the 10X objective in the

5

7

6

Fig. 6

5

light path.

Rotate the coarse and fine adjustment knobs to bring the specimen

6

in focus. (Details: Page 19)

Rotate the stage knob to adjust the observation position.

7

Now you can observe the magnified image of the specimen. To
improve the observation further, read section 4-2, “Microscope
Adjustments” below.

4-2 Microscope Adjustments (How to Improve the Observed Image)

Adjust the microscope as described below to improve the observed image.

1 Adjusting the Interpupillary Distance

While looking through the eyepieces, adjust for binocular vision until

1

the left and right fields of view coincide completely.
} Note your interpupillary distance so that it can be quickly duplica-

1

Fig. 7

2 Adjusting the Diopter

ted.

The diopter adjustment ensures parfocality from low to high magni­fications and makes it possible to avoid defocusing even when the
objective is switched.
For details, see page 19.

Fig. 8

3 Adjusting the Centering

4

3

567

Place the specimen.

1

Rotate the revolving nosepiece to select the 10X objective.

2

Raise the lever to engage the top lens in the light path.

3

Rotate the knobs to bring the specimen in focus.

4

Rotate the knob to raise the condenser to its upper limit.

5

Rotate the field iris diaphragm ring in the direction of the arrow so that the diaphragm image comes inside the field

6

of view.

Rotate the knobs to focus on the field iris diaphragm image (A in Fig. 9).

7

Pull out the two condenser centering screws.

8

Insert the two condenser centering screws into the condenser centering thread holes and turn the two condenser

9

centering screws to move the iris diaphragm image to the center of the field of view (B in Fig. 9).

Gradually open the field iris diaphragm until its image is inscribed in the field of view (C in Fig. 9).

10

8

2

1

10 11

9

Open the field diaphragm slightly until its image circumscribes the field of view slightly (D in Fig. 9).

11

} With the 100X objective, the field iris diaphragm image cannot be observed unless the iris diaphragm is minimized. With

the 4X objective, maximize the iris diaphragm to observe it.

A B C D

Fig. 9 Movement of Field Iris Diaphragm Image

4 Adjusting the Contrast

Aperture iris
diaphragm image

Objective pupil

1

Set the condenser aperture iris diaphragm ring so that the condenser NA scale position indicates 70% of the NA of the

1

objective in use. After this, observe the image and fine adjust the aperture iris diaphragm to the desired condition.

NA scale

70-80%

30-20%

2

12

The image of the aperture iris diaphragm can be viewed by removing the eyepieces and looking into the observation

2

tube. Adjust so that the image looks approximately like that shown in the figure above.

13

BX46

5

USING THE CONTROLS

5-1 Base

1

Fig. 10

1 Adjusting the Brightness

1. When the LED lamp is used for observation, turn the LED brightness
adjustment knob @ clockwise to make illumination brighter.

2. When the halogen bulb is used for observation, adjust the brightness
adjustment knob 2 of the TL4 power supply unit.

(Figs. 10 & 11)

2

1

2

3

4

Fig. 11

Fig. 12

2 Setting the LIM Brightness

} LIM stands for Light Intensity Manager. It is effective when the U-LHLEDC

LED lamp housing is used.

} The desired brightness can be set per objective. Once the brightness for

the objectives is set, selecting an objective adjusts the LED brightness
automatically provided that the LIM ON-OFF switch @ is set to “ON” (which
is indicated by the lighting of the LIM indicator 2).

1. Press the LIM ON-OFF switch @ to “OFF” (so that the LIM indicator 2 is
extinguished).

2. Engage a low-power objective in the light path and bring the specimen
in focus.

3. Rotate the brightness adjustment knob 3 for optimum brightness for
observation.

4. Press the LIM setting switch 4 to enter the current brightness.

5. Set the optimum brightness for the mounted objectives by repeating the
above for each of them.

To modify the LIM setting:

Engage the objective you want to set the new LIM setting in the light path,

set the LIM ON-OFF switch to “OFF”, and perform steps 3 and 4 above.

(Fig. 12)

14

1

3 Using the Filters

} One of the filters listed below can be engaged in the light path by inserting

the filter @ in the filter slider 2 and engaging the filter slider in the light
path.

(Fig. 13)

Fig. 13

2

Usable Filters Applications

32LND1.5/3/6/12/25/50

32LBD For color temperature conversion

32IF550 For B&W contrast (Green)

} The LBD filter is not required when the LED lamp is used.

What is LBD filter?

The color of the sun at the noon of a clear day is called the daylight
color, which looks white to human eyes. On the other hand, the
halogen bulb used as the light source of this microscope does not
emit the daylight-color light. so the LBD filter is engaged in the light
path to convert the color of halogen bulb light into daylight. In general,
engaging the LBD filter makes it possible to observe specimens in
natural color tones.

For light brightness control, transmittance

1.5/3/6/12/25/50%

BX46

5-2 Focusing Block

} The stage of this microscope is fixed at a low height to facilitate replacement of specimens. Take care not to let your

hand and fingers interfere with the stage when operating the coarse adjustment knob.

3

1

5

Fig. 14

4

Fig. 15

1

2

1 Focusing Controls

The focusing block consists of the controls listed in the following table,

which also describes the functions of the controls.

No. Name Function

Coarse adjustment

1

knob

Fine adjustment dial Fine adjusts the focus position.

2

Fine adjustment

3

knob

Pre-focusing lever Sets the lower limit of the objective

4

Tension adjustment

5

ring

Moves the focus position widely.

Fine adjusts the focus position. This knob
can be attached to the fine adjustment
dial on either the left or right side. (When
the microscope is shipped, it is attached
to the right-side dial.)

position (to prevent contact between the
specimen and objective).

Adjusts the force required to rotate the
coarse adjustment knob.

(Figs. 14 & 15)

2

1

Fig. 16

2 Adjusting the Focus

With both the coarse adjustment knob @ and fine adjustment knob 2,

rotating the knob in the direction of the arrow raises the objective (lets
the specimen approach the objective).

(Fig. 16)

15

3 Replacing the Fine Adjustment Knob

(Fig. 17)

1

4

2

1

3

Fig. 17

Fig. 18

5

CAUTION

4

CAUTION

The fine adjustment knob has been attached on the left side at

the factory.

} The fine adjustment knob is designed detachable to prevent interference

with hand during manipulation of the X-and Y-axis knobs.

Usually attach the fine adjustment knob on the opposite side to the X- and

Y-axis knobs.

1. Pull and remove the rubber cap @ from the fine adjustment knob.

2. Using the Allen screwdriver, loosen the clamping screw 2 and remove
the fine adjustment knob 3.

3. Remove the seal from the fine adjustment knob screw hole on the other
side and attach the knob by reversing the removal procedure.

4. Attach a provided seal on the screw hole 5 of the fine adjustment dial
|, from which the fine adjustment knob has been removed.

} The fine adjustment dial | can be operated with your fingertip or finger

surface at the same time as manipulating the X- and Y-axis knobs.

Adjusting the Coarse Adjustment Knob Tension

Adjust the coarse adjustment knob tension using the tension

adjustment ring @.

The coarse adjustment knob tension is preadjusted for easy use. However,

if desired, you can change the tension using the tension adjustment ring
@. Turning the ring in the direction of the arrow increases tension, and
vice versa.

The tension is too low if the stage drops by itself of focus is quickly lost

after adjustment with the fine adjustment knob. In this case, turn the ring
in the direction of the arrow to increase tension.

(Fig. 18)

16

1

Fig. 19

5 Pre-focusing Lever

} The pre-focusing lever ensures that the objective does not come in contact

with the specimen and simplifies focusing.

After focusing on the specimen with the coarse adjustment knob, turn this

lever @ in the direction of the arrow and lock; the lower limit on coarse
adjustment movement is set at the locked position.

After changing a specimen, approximate refocusing is easily accomplis-

hed by rotating the coarse adjustment knob to reach the pre-focused
position. After this, all you have to do is make fine adjustments with the
fine adjustment knob.

} The objective’s vertical movement activated by the fine adjustment knob

is not locked.

} When the pre-focusing lever is locked, a coarse movement stroke is

reduced due to the microscope’s mechanism, causing the revolving
nosepiece not to be able to be lowered to the lower limit. To lower the
revolving nosepiece to the lower limit, release the pre-focusing lever.

(Fig. 19)

BX46

5-3 Stage

} When the U-SP plain stage is used, place the slide specimen directly on the stage because this model does not have

the slide holder.

2

Fig. 20

Fig. 21

1

1 Placing the Specimen

} The dimensions of the slide glass should be 26 x 76 mm with thickness of

0.9 to 1.2 mm, and the cover glass should have thickness of 0.17 mm.

} When observing very large specimens, remove the slide holder and place

the specimen directly on the stage.

Microscopy with Double-Slide Holder (Fig. 20)

1. Turn the coarse adjustment knob @ to upper the objective.

2. Open the spring-loaded curved finger 2 on the slide holder and place
one or two specimen slides on the stage from the front.

3. After placing the sides as far as they will go, gently release the curved
finger.

Microscopy with Single-Slide Holder (Fig. 21)

The specimen side can easily be placed by sliding it into the slide holder

from the front.

(Figs. 20 & 21)

17

18

2 Adjusting the X- and Y-Axis Knob Tension

(Fig. 22)

4

Fig. 22

2

3

1

1. Hold the X-axis knob @ and slide up the Y-axis knob 2 up to expose
the adjustment knobs.

2. Turning the X-axis adjustment knob 3 or Y-axis adjustment knob |
clockwise (in the direction of the arrow) increases the tension and
counterclockwise decreases it.

CAUTION

If the tension is adjusted to tight, a creaking sound may be heard

during stage travel, and the stage stopping accuracy may be
imperiled.

} After long hours of use, the stage guide may be deviated and the stage

travel range may be decreased. However, this is not malfunction and can
be corrected easily as described below.

[Treatment]

Horizontal direction: Hold the specimen holder and move the stage guide

to the left and right so that it hits the stoppers.

Vertical direction: Hold the upper stage and move it to the front and rear

so that it hits the stoppers.

Stage Feed Knob Rubber Caps (Optional)

}When the X- and Y-axis knobs are fitted with the rubber caps, the knobs

can be adjusted without slipping and fine adjustment is possible by
holding the knobs with a very light force. The knob rubber caps also
reduce fatigue after long hours of operation.

The U-SHGT thick type (thickness 5 mm) and U-SHG thin type (thickness

2 mm) knob rubbers are available.

To attach the knob rubbers:

First fit the larger knob rubber to the Y-axis (upper) knob from below it,

then fit the smaller knob rubber to the X-axis (lower) knob from below it.

1

Fig. 23

3 Rotating the Stage

1. Using the Allen screwdriver, slightly loosen the stage clamping screw @.

2. The stage can be rotated both clockwise and counterclockwise by the
stage clamping screw.

CAUTION

A click may be heard and felt during rotation. However, this is

due to the construction of the substage and does not indicate
a malfunction.

} The angle of rotation varies depending on the positions of the X- and

Y-axis knobs.

Angle of Rotation

Clockwise Counterclockwise

Right hand knobs

Left hand knobs 20° 230°

230° 20°

(Fig. 23)

19

BX46

5-4 Observation Tube

1

Fig. 24

2

1 Adjusting the Diopter

1. Set the diopter adjustment rings on both sides to scale “0”.

2. Engage a high-power objective (40X or so) in the light path, look into
the right eyepiece with your right eye, and rotate the coarse and fine
adjustment knobs to bring the specimen into focus.

} Do not use an immersion objective.

3. Engage a low-power objective (10X or so) in the light path, rotate the right
diopter adjustment ring @ to bring the specimen into focus. Do not touch
the coarse and fine adjustment knobs during the focusing.

4. Looking into the left eyepiece with your left eye, rotate the left diopter
adjustment ring 2 to bring the specimen in focus.

} The above procedure adjusts the diopter with reference to the right eye,

but it is also possible to adjust with reference to the left eye. In this case,
read the above procedure by inverting “right” and “left.”

} If an eyepiece equipped with a diopter adjustment ring is inserted into

the side equipped with another diopter adjustment ring of the observation
tube, perform the above procedure without moving the diopter adjustment
ring of the observation tube from the “0” position.

2 Using the Eye Shades

When Wearing Eyeglasses

Use with the eye shades in the normal, folded-down position. This will

prevent the eyeglasses from being scratched.

(Fig. 24)

(Fig. 25)

Fig. 25

When Not Wearing Eyeglasses

Extend the folded eye shades in the direction of the arrow to prevent

extraneous light from entering between the eyepieces and eyes.

20

2

Fig. 26

1

3 Mounting the Eyepiece Micrometer Disk

When the WHN10X-H eyepieces are used, an eyepiece micrometer disk

can be mounted.

Use 24 mm dia. x 1.5 mm thick micrometer disks.
Following Fig. 26, turn the built-in micrometer-mounting frame 2 to the

arrow direction to remove it from the eyepiece and place a micrometer disk
@ into the mounting frame so that the surface with the model indication
faces downward.

} The micrometer-mounting frame may be tight for certain micrometer

disks.

In this case, turn the frame by holding the circumference with a light,

niform force or by applying the frame against a rubber sheet. Do not grasp
the frame with a strong force, as this may deform the frame and make it
harder to remove it.

Re-attach the micrometer mounting frame in the original position.
} Be careful not to touch the lens surface with your finger.

(Fig. 26)

Fig. 27

1

4

Selecting the Light Path of the Trinocular Tube

Slide the light path selector knob @ to select the desired light path.

Trinocular tube

U-TR30-2

100% for binocu­lar eyepieces

50% for binocu-

U-TTR-2**

* With the infrared trinocular tube, infrared observation up to 1000 nm is

possible. For details, consult your Olympus representative.

** The light path selector knob is removable and can be attached to the

other side.

1. Put the edge of a coin in the groove on the tip of the light path selector
knob. Turn the coin clockwise to remove the knob.

2. Remove the cap from the other side.

3. Insert the end of the light selector knob into the hole from which the cap
has been removed. Put the edge of a con in the groove on the other end
of the knob and turn the coin counterclockwise to attach the knob.

4. Close the open hole with the cap.

lar eyepieces,
50% for camera

Light path selector knob position

Pushed in Middle position Pulled out

20% for binocu­lar eyepieces,
80% for camera

50% for binocu­lar eyepieces,
50% for camera

100% for binocu­lar eyepieces

100% for cameraU-TR30NIR*

(Fig. 27)

BX46

5° to 35°

Fig. 28

Fig. 29

1

5 Adjusting the Tilt

Adjust the height and tilt of the observation tube to obtain the most

comfortable viewing position.

U-TBI-3 5° to 35°

U-TBI-3-CLI 5° to 35°

U-ETBI 0° to 25°

U-TTBI 0° to 25°

U-TTR-2 5° to 35°

U-TTLBI 0° to 27°

With the U-TBI-3 (Fig. 28)

Holding the binocular section @ with both hands, raise or lower it to the

desired position.

CAUTION

Never attempt to force the binocular section past the upper or

lower stop position. Applying excessive force could destroy the
limiting mechanism.

} Vignetting or ambient light insufficiency occurs in combination of U-TBI-

3/U-TBI-3-CLI and an additional intermediate attachment.

} If you need photography using with tilting function, please use U-TTR-2

or the combination of U-TTLBI/U-ETBI/U-TTBI and U-TRUS trinocular
intermediate attachment.

(Figs. 28 to 31)

2

Fig. 30

Fig. 31

With the U-ETBI/U-TTBI (Fig. 29)

The U-ETBI and U-TTBI are ergonomic observation tubes with normal

field, capable of the adjustments of the positioning angle (0° to 25°) as
well as front-rear positioning (by 45 mm) of the eyepieces. The U-ETBI is
the erect image model and the U-TTBI is the inverted image model, and
both models are of the same size.

With the U-TTLBI (Fig. 30)

The U-TTLBI is a Tilting, Telescopic, Lifting binocular tube with the following

adjustment capabilities.

· Eyepiece angle adjustment (0° to 27°)

· Eyepiece up-down position adjustment (45 mm)

· Observation tube front-rear position adjustment (55 mm)

} When adjusting front-rear position of the observation tube, hold the base

of the observation tube as shown in Fig. 31.

} When storing the microscope, increase the angle of the eyepieces so as

to prevent the eyepieces from dropping.

} To adjust the up-down position of the eyepiece, turn the dial 2.

CAUTION

The intermediate attachments that can be combined with the

U-TTBI and U-TTLBI are limited. For details, please contact
Olympus.

21

22

1

Fig. 32

2

6

Using Eyepieces Incorporating a Micrometer

} When the eyepieces in use incorporate a micrometer, the accuracy of

the left-right focusing adjustment (diopter adjustment) can be improved
further.

1. Looking into the right eyepiece with your right eye, turn the top of the
eyepiece @ so that the micrometer in the field of view looks sharpest
(Fig. 32).

2. Looking into the right eyepiece, rotate the coarse and fine adjustment
knobs to bring the specimen and micrometer (cross lines, etc.) into
focus.

3. Looking into the left eyepiece with your left eye, rotate the diopter
adjustment ring 2 to bring the specimen into focus.

5-5 Condenser

For how to adjust the centering of the condenser, see page 12.

1 Compatibility of Objectives and Condensers

Objective Magnification BX45 Condenser Field iris diaphragm Aperture iris diaphragm

2X/4X Applicable by swing the top lens out. Used as the aperture

10X/20X/40X Applicable by engaging the top lens

in the light path.

(Fig. 32)

Opened

iris diaphragm

Field iris diaphragm Aperture iris diaphragm

} The 1.25X to 2X objectives can also be used but the surroundings of the field of view may become dark.

23

BX46

5-6 Immersion Objectives

CAUTION

Be sure to use the provided Olympus Immersion oil.

1 Using Immersion Objectives

1. Focus on the specimen with objectives in the order of lower-power to
higher-power ones.

2. Before engaging the immersion objective, place a drop of provided
immersion oil onto the specimen at the area to be observed.

3. Turn the revolving nosepiece to engage the immersion objective, then
focus using the fine adjustment knob.

CAUTION

Fig. 34

Since air bubbles in the oil will affect the image quality, make

sure that the oil is free of bubbles.

a. To check for bubbles, remove the eyepiece and fully open the field and

aperture iris diaphragms, then look at the exit pupil of the objective inside
the observation tube. (The pupil should appear round and bright.)

b. To remove bubbles, turn the revolving nosepiece to repeatedly defocus

and refocus the immersion objective.

} If the condenser engraving shows a numerical aperture (NA) of 1.0 or

higher, the number applies only when oil is applied between the slide
glass and the top surface of the condenser. When oil is not present, the
NA is about 0.9.

4. After use, remove immersion oil from the objective front lens by wiping
with gauze slightly moistened with absolute alcohol.

(Fig. 34)

CAUTION

Caution in use of immersion oil

If immersion oil enters your eyes or contacts with your skin, imme­diately take the following treatment.
Eyes: Rinse with fresh water (for 15 minutes or more).
Skin: Rinse with water and soap.
If the appearance of the eyes or skin is altered or pain persists,
immediately see your doctor.

6

6

} Use a trinocular observation tube such as the U-TTR-2 when recording video images or digital camera images of

The trinocular tube accepts a camera adapter (certain camera adapters necessitate a camera mount adapter).
} Be sure to adjust the parfocality before using a camera adapter. Otherwise, the focusing of the camera image will not

For details, refer to the instruction manuals for these accessories.

1 System Chart

CAMERA RECORDING

microscope images.

match that of the image observed through eyepieces. For the parfocality adjustment method, refer to the instruction
manual for the camera adapter in use.

TV camera/digital camera

Camera mount adapter

C-mount camera adapter

Camera adapter

Trinocular observation tube
U-TTR-2

2 Selecting the Camera Adapter Magnification

The image pickup area is determined by the size of the image sensor used in the TV camera or digital camera and the

magnification of the camera adapter.

The following illustrations show the image pickup areas of camera adapters when the SWH10X-H eyepieces with a FN

of 26.5 are used (the FN of the WHN10X eyepieces is 22).

1-inch image sensor

2/3-inch image sensor

1/2-inch image sensor

WHN10X: FN22

24

When a 0.5X camera adapter is used When a 1X camera adapter is used

25

BX46

7

Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems

If you cannot solve the problem after checking the entire list, please contact your local Olympus representative for

Bulb does not light.

Bulb is blown immediately. Halogen The bulb is not a designated

Field of view is dark.

Brightness cannot be varied.

Field of view is obscured or not
evenly illuminated.

TROUBLESHOOTING GUIDE

occur, please review the following list and take remedial action as needed.

assistance.

Problem Lamp Cause Remedy Page

LED Power is not ON.

Common Power cord is unplugged. Plug power cord into the power

Common Connectors are connected im-

properly.

Halogen Bulb is not attached. Attach a halogen bulb.

Halogen Bulb is burned out. Replace bulb.

Halogen The TL4 is not ON. Set the main switch of the TL4

one.

LED LIM setting is inappropriate. Set LIM again.

Common Aperture and field iris diaphragms

are not opened wide enough.

Common

Common Condenser is not properly cen-

Common Condenser height is too low. Adjust correctly.

Common ND filter is engaged. Disengage the ND filters.

LED LIM is not ON. Switch the LIM function OFF.

Halogen LED brightness adjustment knob

Common Condenser is no t properly

Common Condenser height is too low. Adjust correctly.

Common Light path selector knob is set in

Common Objective is not correctly enga-

Common Condenser top lens is not swit-

Common An objective that falls outside

Common Condenser is no t properly

Common Field iris diaphragm is stopped

Halogen Bulb is not mounted correctly. Push the pins of halogen bulb all

Light path selector knob is set to

position.

the

tered.

is rotated.

centered.

an intermediate position.

ged.

ched properly.

of the condenser’s illumination
range is used.

centered.

down too far.

Set the main switch to “ I ” (ON).

outlet.
Connect connectors securely.

to “ I ” (ON).
Replace bulb with a designated

one.

Adjust them to proper sizes.

Set the knob to the
position.

Center the condenser.

Use the brightness adjustment
knob of the TL4.

Center the condenser.

Set to the desired click position.

Make sure that the revolving
nosepiece clicks properly into
place.

Swing out the top lens when
us ing a 4X or highe r-powe r
objective.

Use the objective with the specified
magnification.

Center the condenser.

Open the field iris diaphragm to
an optimum size.

the way until the stop position.

or

10

34

32

32

32

7

1

13

12

20

12

12

14

13

7

12

12

20

–

12

30

12

12

32

26

Problem Lamp Cause Remedy Page

Dirt or dust is visible in the field
of view.

Visibility is poor

· Image is not poor.

· Contrast is poor.

· Details are indistinct.

· Image glares.

One side of image is blurred. Common Objective is not correctly engaged

Image appears to waver. Common Objective is not correctly engaged

Coarse adjustment knob is hard
to turn.

Image cannot be focused:

· Objective drifts down by itself.

· Fine adjustment knob slips.
Coarse adjustment will not go all

the way down.
Objective makes contact with

specimen before focus is
obtained.

Common Dirt/dust on the eyepieces.

Common Dirt or dust on condenser top

lens.

Common Dirt/dust on the specimen.

Common A non-UIS/ UIS 2 obj ective is

used.

Common Condenser height is too low. Adjust correctly.

Common Aperture iris diaphragm is not

opened enough.

Common Front lens of objective is dirty. Clean objective.

Common Immersion oil is not being used

with an oil immersion objective.

Common Immersion oil contains bubbles. Remove the bubbles.

Common Recommended immersion oil is

not used.

Common Dirt/dust on specimen.

Common Dirt/dust on condenser top lens.

in light path.

Common Stage is not correctly mounted. Re-attach it.

Common Specimen is mounted upside

down.

in light path.

Common Condenser is no t properly

centered.

Common Ten s ion ad justment ring is

tightened excessively.

Common You are trying to raise stage

without coarse adjustment knob
while pre-focusing lever is kept
locked.

Common Tension adjustment ring is too

loose.

Common Pre-focusing lever is locked at a

high position.

Common Specimen is mounted upside

down.

Clean thoroughly.

Use only UIS2/UIS series objectives
with this microscope.

Open aperture iris diaphragm.

Use immersion oil.

Use an Olympus-designated
immersion oil.

Clean it.

Make sure that revolving nosepiece
clicks into place correctly.

Mount specimen correctly.

Make sure that revolving nosepiece
clicks into place correctly.

Center the condenser.

Loose ring.

Unlock pre-focusing lever.

Tighten ring.

Unlock it.

Mount specimen correctly.

3

30

12

12

3

23

23

23

3

–

18

–

–

12

16

16

16

16

–

27

BX46

Problem Lamp Cause Remedy Page

Field of view of one eye does not
match that of the other.

Image shifts when you touch
stage.

X- and Y-axis knobs are too tight
or too loose.

Stroke has reduced. Common Stage guide is deviated. Correct deviation as described.

Common Interpupillary distance is incorrect. Adjust interpupillary distance.

Common Diopter adjustment is incorrect. Adjust diopter.

Common Different eyepieces are used on

left and right.

Common Your view is not accustomed to

microscope observation.

Common Stage is not properly mounted. Clamp stage.

Common Tension of X- and Y-axis knobs is

too high or too low.

Change one eyepiece to match
the other so that both sides are
the same type.

Upon looking into eyepieces,
try looking at overall field before
concentrating on specimen range.
You may also find it helpful to
look up and into distance for a
moment before looking back into
microscope.

Adjust tension.

11

19

33

–

18

18

18

8

8

1. Optical system UIS2 (UIS) optical system (featuring infinity correction)

2. Illumination Built-in transmitted Koehler illumination

3. Focusing mechanism Revolving nosepiece movement by roller guide (rack & pinion)

4. Revolving nosepiece 5-position revolving nosepiece with information readout function (fixed on microscope frame).

5. Observation tube Type

6. Stage Type

SPECIFICATIONS

Item Specification

FN (Field Number): 22 (widefield compatible)
LED illuminator (continuously variable): U-LHLEDC

Optional 6V 30W halogen bulb (continuously variable): 6V30WHAL (PHILIPS 5761)
Power supply unit TL4: 100-120/220-240 V $ , 0.85/0.45 A, 50/60 Hz.
Power consumption: 45 W

Stroke per rotation: 0.1 mm (fine), 17.8 mm (coarse)
Full stroke range: 15 mm
Higher coarse adjustment limit stopper
Tension adjustment on coarse focus adjustment knob.

With U-GAN insertion slot.
LIM setting possible (when the LED lamp is used)

U-BI30-2 U-TBI-3/ U-TBI3-CLI U-TTLBI

Widefield binocular Widefield, tilting binocular

Field No. 22

Tube
inclination

Interpupillary
distance
adjustment

U-SVRC/U-SVRC-CY U-SVLC U-SP

Coaxial knobs on bottom
right.

30°

Rectangular, ceramic-coated, wire-driven stage –

(continuously variable)

50-76 mm

Coaxial knobs on bottom
left.

5-35°

Widefield, lift & telescopic
binocular

0°-27°

(continuously variable)

Plain

28

Size 156 mm (D) x 191 mm (W) 150 mm (D) x 180 mm (W)

Movement
mechanism

7. Slide holder Single-slide
holder

Double-slide
holder

X- and Y-axis knob with adjustable tension. Movement
range: 52 mm in vertical (Y) direction, 76 mm in horizontal
(X) direction.

U-HLST-4 (Thick type)

U-HLS-4 (Thin type)

U-HLDT-4 (Left-opening, thick type)

U-HLD-4 (Left-opening, thin type)

U-HRDT-4 (Right-opening, thick type)

U-HRD-4 (Right-opening, thin type)

–

29

BX46

Item Specification

8. Condenser

9. Operating

environment

10. Transport / Storage

environment

11. Rating

Type Universal condenser (fixed on frame)

N.A. 0.9

Aperture iris
diaphragm

Filter

Observation
method,
applicable
objective
powers

· Indoor use.

· Altitude: Max. 2000 meters

· Ambient temperature: 5° to 40°C (41° to 104°F)

· Maximum relative humidity: 80% for temperatures up to 31°C (88°F), decreasing linearly through
70% at 34°C (93°F), 60% at 37°C (99°F), to 50% relative humidity at 40°C (104°F).

· Supply voltage fluctuations: ±10%

· Pollution degree: 2 (in accordance with IEC60664-1)

· Installation/Overvoltage category: II (in accordance with IEC60664-1)

Temperature: Min. –25°C, Max. 65°C
Humidity: Min. 0%, Max. 90%

Input rating: 5 V, 1.5 A
AC adapter SA115B-05V: 100V-240Vac 50/60 Hz, 0.4 A

A

32 mm filter can be engaged in the light path (using the filter slider).

(Note) The top lens should be swung out when using the 2X or 4X objective.

Graduated scale provided

Transmitted light brightfield observation,

2X - 40X widefield (FN 22).

30

9

OPTICAL CHARACTERISTICS (UIS2 Series)

UIS series objectives not listed here can also be combined with this microscope.

The following table shows the optical characteristics of com-

binations of eyepieces and objectives. The figure on the right
shows the performance data engraved on the objectives.

NOTE

Refer to the latest catalogue or consult your local Olympus

representative for the updated information on the eyepieces
and objectives that can be combined with this microscope.

Optical character

Objective
Series Marking

PLN (PH)
Plan
Achromat
(FN 22)

PH: Phase
contrast

UPLFLN (PH)
Plan Semi
Apochromat
(FN 26.5)

PH: Phase
contrast

UPLSAPO
Plan
Apochromat
(FN 26.5)

PLAPON
Plan
Apochromat
(FN 26.5)

UAPON
Apochromat
(FN 22)

PlanN (Ph) 2X

UPlanFLN
(Ph)

UPlanSApo 4X

PlanApoN

UApoN 20XW340

Magnifi-

cation

4X
10X (Ph)
20X (Ph)
40X (Ph)
50XOI

100XO (Ph)

4X (Ph)
10X2 (Ph)
20X (Ph)
40X (Ph)
40XO
60X
60XOI

100XO2
100XOI2

10X2
20X
20XO
30XS
40X2
60XW
60XO
60XS

100XO

1.25X
2X
60XO

40XW340

40XO340

(Ph)
(Ph)

N.A.

0.06

0.1

0.25

0.4

0.65

0.5-0.9

1.25

0.13

0.3

0.5

0.75

1.3

0.9

0.65-1.25

1.30

0.6-1.30

0.16

0.4

0.75

0.85

1.05

0.95

1.2

1.35

1.3

1.4

0.04

0.08

1.42

0.7

1.15

1.35

W.D.

(mm)

5.8

18.5

10.6

1.2

0.6

0.2

0.15

17.0

10.0

2.1

0.51

0.2

0.2

0.12

0.2

0.2

13.0

3.1

0.6

0.17

0.8

0.18

0.28

0.15

0.3

0.13

5.0

6.2

0.15

0.35

0.25

0.1

Mechanical tube
length

Cover

glass
thick-

ness

(mm)

–
–
–

0.17

0.17

0.17
–

–
–

0.17

0.17

0.17

0.17

0.17

0.17

0.17

–

0.17

0.17
–

0.13-0.19

0.17

0.17

0.17

0.15-0.19

0.17

–
–

0.17

0.17

0.13-0.25

0.17

Magnification

Color band

Reso-

lution

(µm)

5.59

3.36

1.34

0.84

0.52

0.37

0.27

2.58

1.12

0.67

0.45

0.26

0.37

0.27

0.26

0.26

2.10

0.84

0.45

0.39

0.32

0.35

0.28

0.25

0.26

0.24

8.39

4.19

0.24

0.48

0.29

0.25

Total mag.

20X
40X

100X
200X
400X
500X

1000X

40X

100X
200X
400X
400X
600X
600X

1000X
1000X

40X

100X
200X
200X
300X
400X
600X
600X
600X

1000X

12.5X
20X
600X

200X

400X

400X

Eyepiece

WHN10X (FN22)

Depth of

focus

(µm)

560.1

175.0

28.0

9.27

3.04

1.7

0.69

127.2

22.4

7.0

2.52

1.27

1.5

0.98

0.66

0.66

99.6

15.9

4.29

3.5

2.07

1.9

1.03

0.89

0.93

0.59

1326.8

398.3

0.83

4.08

1.29

1.2

Objective type

Numerical aperture

Oil: Oil immersion
Iris: Iris diaphragm

Field number (FN)

Cover glass thickness

Field of

view

11.0

5.5

2.2

1.1

0.55

0.44

0.22

5.5

2.2

1.1

0.55

0.55

0.37

0.37

0.22

0.22

5.5

2.2

1.1

1.1

0.73

0.55

0.37

0.37

0.37

0.22

17.6

11.0

0.37

1.1

0.55

0.55

Remark

Oil immersion/Iris
Oil immersion

Oil immersion
Correction collar
Oil immersion/Iris
Oil immersion
Oil immersion/Iris

Oil immersion
Silicone immersion
Correction collar
Water immersion
Oil immersion
Silicone immersion
Oil immersion

Oil immersion

Water immersion
Water immersion/

Correction collar
Oil immersion

BX46

10

10

ASSEMBLY

10-1 Assembly Diagram

The diagram below shows the sequence of assembly of the various modules. The numbers indicate the order of

assembly.

The module numbers shown in the following diagram are merely the typical examples. For the modules with which the

module numbers are not given, please consult your Olympus representative or the catalogues.

CAUTION

When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching

any parts or touching glass surfaces.

Assembly steps enclosed in
} Most assembly operations are possible by using the Allen screwdriver (

Eyepieces
WHN10X
WHN10X-H
CROSS-WHN10X, etc.

will be detailed on the subsequent pages.

Observation tube

6

U-BI30-2
U-TTLBI
U-TTR-2, etc.

) provided with the microscope.

Intermediate attachment
U-CA
U-APT
U-EPA2
U-ECA
U-DA, etc.

Slide holder
U-HLD-4
U-HRD-4, etc.

Stage
clamping
screw

Stage
U-SVRC-CY
U-SVRC
U-SVLC
U-SRG2, etc.

4

Gout analyzer
U-GAN

Slide holder
clamping
knobs

UIS2 series
objective

3

Stage feed knob
rubber caps
U-SHG
U-SHGT

Revolving nosepiece

5

Observation tube
clamping screw

cable

Halogen bulb
6V30WHAL

2

7

7

8

AC adapter

32 mm

filter

Filter frame

LS30 adapter
U-LS30ADP

2

1

Power supply unit
TL4

Power cord

LED lamp
housing
U-LHLEDC

2

Lamp socket
U-LS30-5

2

Lamp Cable

2

Power cord

9

9

Polarizer
BX45-PO

31

10-2 Detailed Assembly Procedures

1

2

1

5

Fig. 35

Fig. 36

3

4

2

1 Attaching the LED Lamp Housing

1. Fit the LED lamp housing @ into the mount hole on the rear of the
microscope by aligning the clamping screw 2 and screw hole 3.

2. Using the Allen screwdriver, tighten the clamping screw 2.

3. Insert the connector | of the lamp housing into the connector 5 on the
microscope.

2 Attaching the Halogen Lamp Socket

Installing the U-LS30ADP LS30 Adapter (Fig. 36)

1. Peel off the backing of double-side adhesive tape on the cable holder
@ and attach the holder on the specified position.

2. Attach the adapter in the same way as steps 1 and 2 in the LED lamp
housing C attaching procedure described above.

Installing the Halogen Bulb (Fig. 37)

Use only the designated bulb 6V30WHAL (PHILIPS 5761).

1. Holding the bulb 2 a piece of gauze, insert the bulb pins 3 straight and
fully into the pin holes | on the lamp socket.

CAUTION

To prevent reduced bulb life or cracking, do not touch the bulb

with bare hands. If fingerprints are accidentally left on the bulb,
wipe the bulb with a soft cloth.

(Figs. 36 to 38)

(Fig. 35)

32

3

4

7

Fig. 37

Fig. 38

5

8
9

6

2. Insert the lamp cable 5 in the direction shown in the figure 38.

3. Aligning the guide pins 6 with the guide pin holes 7 at the rear of the
microscope frame, gently push the lamp socket all the way into place.

CAUTION

Caution for Bulb Replacement During Use or Right After Use

The bulb and the lamp socket are areas near these will be

extremely hot during and right after use.

Set the main switch to “

from the wall outlet, then allow the old bulb and lamp socket
to cool before replacing the bulb with a new of the designated
type.

4 Pass the lamp cable through the cable holder @ (Fig. 36).

Connecting the Lamp Cable (Fig. 38)

Connect the cable connector 8 to the connector 9 of the TL4 power

supply unit.

” (OFF), disconnect the power cord

33

BX46

Fig. 39

1

2

6 Attaching the Eyepieces

Gently insert the eyepieces all the way into the eyepiece sleeves.

CAUTION

· When using the U-BI30-2 binocular tube, eyepieces with a po-

sitioning pin cannot be attached because the U-BI30-2 does
not have the positioning notch.

· When using an eyepiece with micrometer or cross lines, attach

it to the right-hand eyepiece sleeve.

When doing so, make sure that the eyepiece positioning pin @

fits into the notch 2 on the eyepiece sleeve.

7 Attaching the Objectives

Screw in the objectives into the holes of revolving nosepiece by following

the position number, beginning with the lowest-power objective toward
higher-power objectives.

} If you want to observe images of 10X and 40X objectives alternately, attach

them side by side.

Revolving Nosepiece Cable

The revolving nosepiece cable is led out of the microscope’s rear panel.

Connect it to the connector on the rear panel.

(Fig. 39)

34

8 Attaching the AC Adapter

(Fig. 40)

Fig. 40

1

Fig. 41

2

2

1

CAUTION

CAUTION

9 Attaching the Power Cord

CAUTION

CAUTION

CAUTION

· Cables and cords are vulnerable when bent or twisted. Never

subject them to excessive force.

· Make sure that the main switch is set to “

connecting the power cord (Fig. 40).

1. Insert the connector @ of the AC adapter firmly into the connector 2 on
the microscope.

Always use the designated AC adapter. Using a non-designated

AC adapter may cause malfunction.

Always use the power cord provided by Olympus. If no power

cord is provided with the microscope, please select the proper
power cord by referring to section “ PROPER SELECTION OF THE
POWER SUPPLY CORD ” at the end of this instruction manual.

Operation Using the AC Adapter (Fig. 41)

1. Insert the connector @ of the power cord firmly into the input connector

2 of the AC adapter (Fig. 41).

The power cord should be connected to a grounded/earthed,

3-conductor power outlet. If the power outlet is not grounded/
earthed properly, Olympus can no longer warrant the electrical
safety performance of the equipment.

2. Plug the power cord plug 3 into the wall outlet 4 (Fig. 43).

Should the power cord come in contact with the hot lamp socket,

the power cord could melt and cause electric shock. Be sure
to route the power cord away from the lamp socket.

” (OFF) before

(Figs. 41 to 43)

2

Fig. 42

Fig. 43

Operation Using the TL4 Power Supply Unit (Fig. 42)

} The following operation is not required when the LED lamp is used.

1

1. Insert the connector @ of the power cord firmly into the input connector
2 of the TL4 (Fig. 42).

2. Plug the power cord plug 3 into the wall outlet 4 (Fig. 43).

43

35

BX46

11

{ For safe use of the lamp socket, we recommend performing the following inspection periodically (every time you replace

the lamp bulb and at least every 6 months).
{ The table below identified the check items to be observed. Put (X) if not applicable or (
{ If there is any (

the lamp soket.
{ If you detect an abnormality other than that listed below or with other Olympus product, also stop the use of the product and

contact Olympus for detailed inspections.
{Note that the service, replacement and detailed inspections are charged after expiration of the warranty period.

1. More than 8 years have passed since original purchase or the total power
ON time exceeds 20,000 hours.

2. Lamp does not light sometimes even though the main switch is set to on.

3. Illumination flickers when you move the lamp cable or lamp socket.

4. Scorching or burning odor is produced during use.

5. Illumination still flickers after replacement with a new lamp bulb.

6. Deformation, backlash, or looseness, etc. when you assemble the lamp socket.

HALOGEN LAMP SOCKET INSPECTION SHEET

) if applicable.

) mark noted, immediately stop use of the product, and consult Olympus for detailed inspections or replace

If you have any questions, please contact Olympus.

Check results (Date)

Check items / / / /

7. Extreme discoloration of the lamp socket connection terminal or lamp socket
lamp bulb mount.

8. Discoloration, deformation or cracking of the lamp socket.

9. Melting, crack, deformation or solidification of the lamp cable or a wiring part.

10. Increased frequency of servicing compared to similar devices put into use at
the same time as the lamp socket.

* When the Check Result columns become insufficient, copy this sheet.

36

n PROPER SELECTION OF THE POWER SUPPLY CORD

If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and
“ Certified Cord ” below:

CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the

electrical safety of the equipment.

Specifications

Voltage Rating
Current Rating
Temperature Rating
Length
Fittings Configuration

125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area)
6A minimum
60˚C minimum

3.05 m maximum
Grounding type attachment plug cap. Opposite terminates in molded-on IEC
configuration appliance coupling.

Table 1 Certified Cord

A power supply cord should be certified by one of the agencies listed in Table 1 , or comprised of cordage marked with an
agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of agencies listed in
Table 1. In case you are unable to buy locally in your country the power supply cord which is approved by one of the
agencies mentioned in Table 1, please use replacements approved by any other equivalent and authorized agencies in
your country.

Country Agency

Argentina

Australia SAA Japan

IRAM Italy IMQ

Certification

Mark

Country Agency

JET, JQA, TÜV,
UL-APEX/MITI

Certification

Mark

Austria ÖVE

Belgium CEBEC Norway NEMKO

Canada CSA Spain AEE

Denmark DEMKO Sweden SEMKO

Finland FEI Switzerland SEV

France UTE

Germany VDE U.S.A. UL

Ireland NSAI

Netherlands KEMA

United
Kingdom

ASTA
BSI

37

BX46

Table 2 HAR Flexible Cord

APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS

Printed or Embossed Harmoni-

Approval Organization

Comite Electrotechnique Belge
(CEBEC)

Verband Deutscher Elektrotechniker
(VDE) e.V. Prüstelle

Union Technique de I´Electricite´
(UTE)

Instituto Italiano del Marchio di
Qualita´ (IMQ)

British Approvals Service for Electric
Cables (BASEC)

N.V. KEMA KEMA-KEUR <HAR> 10 30 30

SEMKO AB Svenska Elektriska
Materielkontrollanstalter

Österreichischer Verband für
Elektrotechnik (ÖVE)

Danmarks Elektriske Materialkontroll
(DEMKO)

zation Marking (May be located
on jacket or insulation of internal
wiring)

CEBEC <HAR> 10 30 10

<VDE> <HAR> 30 10 10

USE <HAR> 30 10 30

IEMMEQU <HAR> 10 30 50

BASEC <HAR> 10 10 30

SEMKO <HAR> 10 10 50

<ÖVE> <HAR> 30 10 50

<DEMKO> <HAR> 30 10 30

Alternative Marking Utilizing
Black-Red-Yellow Thread (Length
of color section in mm)

Black Red Yellow

National Standards Authority of Ireland
(NSAI)

Norges Elektriske Materiellkontroll
(NEMKO)

Asociacion Electrotecnica Y
Electronica Espanola (AEE)

Hellenic Organization for
Standardization (ELOT)

Instituto Portages da Qualidade
(IPQ)

Schweizerischer Elektro
Technischer Verein (SEV)

Elektriska Inspektoratet SETI <HAR> 10 30 90

Underwriters Laboratories Inc. (UL) SV, SVT, SJ or SJT, 3 X 18AWG
Canadian Standards Association (CSA) SV, SVT, SJ or SJT, 3 X 18AWG

<NSAI> <HAR> 30 30 50

NEMKO <HAR> 10 10 70

<UNED> <HAR> 30 10 70

ELOT <HAR> 30 30 70

np <HAR> 10 10 90

SEV <HAR> 10 30 90

MEMO

Printed in Japan on May 26, 2011 M 010-07