Olympus BX43 User manual

INSTRUCTIONS

BX43

SYSTEM MICROSCOPE

This instruction manual is for the Olympus System Microscope Model BX43.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use
of this microscope, we recommend that you study this manual thoroughly before operating the
microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.

A X 7 8 5 1

In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equipment,
this symbol indicates that the product must not be disposed of as unsorted municipal waste, but
should be collected separately.
Refer to your local Olympus distributor in EU for return and/or collection systems available in your
country.

NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,

pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.

Operation of this equipment in a residential area is likely to cause harmful interference in which case

the user will be required to correct the interference at his own expense.

FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance

could void the user's authority to operate the equipment.

BX43

CONTENTS

Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble
the microscope yourself, please read section 10, “ASSEMBLY” (pages 33 to 37) carefully.

IMPORTANT -- Be sure to read this section for safe use of the equipment. --

1 MODULE NOMENCLATURE

2 CONTROLS

3 FLOW OF OBSERVATION

4 SIMPLIFIED OBSERVATION PROCEDURE

4-1 Basic Operation (Until Observation of Specimen) ..................................................................... 10,11

4-2 Microscope Adjustments (How to improve the Observed image) .....................11,12

1 Adjusting the Interpupillary

Distance ................................................................................

3 Adjusting the Centering .................................

2 Adjusting the Left and Right

Focusing ...............................................................................

11

4 Adjusting the Contrast .....................................

12

5 USING THE CONTROLS

5-1 Base ..............................................................................................................................................................................................................13-15

1-3

4

5-7

8,9

10-12

11
12

13-25

1 Adjusting the Brightness ..............................13 2 Setting the LIM Brightness .........................

3 Using the Filters (Halogen Bulb

Operation Only) ..........................................................

14

13

5-2 Focusing Block ...............................................................................................................................................................................16,17

1 Focusing Controls ..................................................

3 Replacing the Fine Adjustment

Knob ............................................................................................

5 Pre-focusing Lever ..................................................

2 Adjusting the Focus .............................................

16

4 Adjusting the Coarse Adjustment

Knob Tension .................................................................

17
17

16

17

5-3 Stage............................................................................................................................................................................................................18-24

1 Placing the Specimen ......................................

3 Adjusting the X- and Y-Axis

Knob Tension ................................................................

5 Adjusting the Stage Height .....................

2 Using the Auxiliary Slide Holder.........

18

4 Rotating the Stage ...............................................

19
20

18

20

5-4 Observation Tube................................................................................................................................................................... 21-24

1 Adjusting the Diopter ....................................... 21 2 Using the Eye Shades ....................................

3 Using the Eyepiece Micrometer

Disk ............................................................................................

5 Adjusting the Tilt .....................................................

4 Selecting the Light Path of the

Trinocular Tube ........................................................

22

6 Using Eyepieces Incorporating a

23

Micrometer ......................................................................

21

22

24

5-5 Condenser ................................................................................................................................................................................................... 24

1 Compatibility of Objectives and

Condensers ...................................................................

24

5-6 Immersion Objectives ................................................................................................................................................................. 25

1 Using Immersion Objectives ................. 25

5-7 Objectives with Correction Collar .............................................................................................................................. 25

6 CAMERA RECORDING

1 System Chart

......................................................... 26

2 Selecting the Camera Adapter
Magnification

......................................................... 26

7 TROUBLESHOOTING GUIDE

8 SPECIFICATIONS

9 OPTICAL CHARACTERISTICS

(UIS2 Series)

10 ASSEMBLY -- See this section for the replacement of the light bulb. --

11 HALOGEN LAMP SOCKET INSPECTION SHEET

PROPER SELECTION OF THE POWER SUPPLY CORD .......................................................... 39,40

26

27-29

30,31

32

33-37

38

IMPORTANT

BX43

SAFETY PRECAUTIONS

(Fig. 1)

1. After the equipment has been used in an observation of a specimen that
is accompanied with a potential of infection, clean the parts coming in
contact with the specimen to prevent infection.

· Moving this product is accompanied with the risk of dropping the

specimen. Be sure to remove the specimen before moving this
product.

· In case the specimen is damaged by erroneous operation, promptly

take the infection prevention measures.

· Follow the procedures described in Chapter “Getting Ready” (see on

page 2) prior to using the accessories of this product. Otherwise, the
stability of the device will be lost and the dropped specimen will cause
the possibility of infection.

2. Install the microscope on a sturdy, level table or bench.

3. Be careful not to tilt the microscope too much. Otherwise, the mobile
parts such as the light path selector knob may move in unintended
directions.

4. Always use the power cord provided by Olympus. If no power cord is
provided, please select the proper power cord by referring to the section
“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of
this instruction manual.

If the proper power cord is not used, product safety performance cannot

be warranted.

5.

Always ensure that the grounding terminal of the microscope and that of the
wall outlet are properly connected. If the equipment is not grounded, Olympus
can no longer warrant the electrical safety performance of the equipment.

1

Fig. 1

Operation Using the LED Lamp

Safety Note on LED (Light Emitting Diode)

The LED incorporated in the LED lamp housing is a class 1 LED
product as defined in the applicable standard. The light emitted by LED
is basically safe, but do not look at the illumination light directly for an
extended period to prevent your eye from being injured

CLASS 1 LED PRODUCT

Operation Using the Halogen Bulb

1. To avoid potential shock hazards and burns when replacing the halogen
bulb, set the main switch of the TL4 power supply unit to “
allow the lamp socket @ and bulb to cool before touching them.

Designated bulb 6V30WHAL (PHILIPS 5761)

CAUTION

Always use the designated lamp bulb. Using other bulb may

cause malfunction.

2. If the lamp cable or power cord comes in contact with the lamp socket
or its surroundings, the cable or cord may be melt by the heat, causing
an electric shock or fire hazard. To prevent this, distribute the lamp cable
and power cord at enough distances from the lamp socket.

3. The standard service life of the lamp housing is eight (8) years of use or
20,000 hours of total power ON period, whichever is the shorter period.

For details, see Inspection Sheet on page 38.

” (OFF) and

1

2

Safety Symbols

The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment
in the safest possible manner.

Symbol Explanation

Indicates that the surface becomes hot, and should not be touched with bare hands.

Indicates a non-specific general hazard. Follow the description given after this symbol or in
instruction manual.

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

Caution engraving/label

Caution engraving and label are placed at parts where special precaution is required when handling and using the

microscope. Always heed the cautions.

Caution engraving
position

Caution label
position

If a caution engraving or label is dirty or peeled off, contact Olympus for the replacement or other inquiry.

Halogen lamp socket
[Caution against high temperature]

Rear panel
[Caution against high temperature]

1 Getting Ready

1. A microscope is a precision instrument. Handle it with care and avoid
subjecting it to sudden or severe impact. Also note that the microscope
does not have a waterproof construction.

2. Do not use the microscope where it is subjected to direct sunlight, high
temperature and humidity, dust or vibrations. (For the operating conditions,
refer to section 8, “SPECIFICATIONS”.)

3. When moving the microscope, remove the observation and specimen
and carefully carry the microscope by holding the grasping part on the
rear of the arm and the front part of the base as shown in Fig. 2.

CAUTION

Fig. 2

To prevent damage to the microscope, never hold it by the stage

or coarse/fine adjustment knob.

· Even when moving the microscope along the desktop surface, be sure
to remove the specimen in advance.

· When the microscope needs to be packaged for forwarding to a remote
location, contact Olympus.

4. When the accessories of this product are used, make sure that the system
height does not exceed 1 meter. Otherwise, the stability of the device will
be lost.

3

BX43

2 Maintenance and Storage

1. To clean the lenses and other glass components, simply blow dirt away using a commercially available blower and wipe
gently using a piece of cleaning paper (or clean gauze).

If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute

alcohol.

CAUTION

· Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks --- for example, electrical

equipment that is being switched on or off, which could cause ignition of a fire.

Also remember to always use it only in a well-ventilated room.

· Set the main switch to “
to maintenance.

2. Do not use organic solvents to clean the microscope components other than the glass components. To clean them,
use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.

3. Do not disassemble any part of the microscope except for the parts that are specified to be disassembled in this manual,
as this could result in malfunction or reduced performance.

4. After using the microscope, set the main switch to “
halogen bulb has been used,) and keep it covered with a dust cover during storage.

5. Before disposing of this product, be sure to follow the regulations and rules of your local government.

” (OFF) and wait for the lamp housing to cool down sufficiently before proceeding

” (OFF), (wait for the lamp socket to cool down sufficiently if the

3 Caution

If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,

the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.

CAUTION

: Indicates a potentially hazardous situation which, if not avoided, may result in minor

or moderate injury or damage to the equipment or other property. It may also be used
to alert against unsafe practices.

} : Indicates commentary (for ease of operation and maintenance).

4 Intended use

This instrument has been designed to be used to observe magnified images of specimens in various routine work and

research applications.

Do not use this instrument for any purpose other than its intended use.
This instrument is considered a medical device in the following countries: US, and China.

This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic
medical devices. CE marking means the conformity to the directive.

4

1

} The modules mentioned below show only the typical product names. As there are some products that are not mentioned

For the products marked “ * ”, also read their instruction manuals.

MODULE NOMENCLATURE

but also applicable to this microscope, check the latest catalogues or consult Olympus.

Observation tube

· U-BI30-2

· U-TBI-3

· U-TR30-2

· U-TTLBI

Eyepieces

· WHN10X

· WHN10X-H

Revolving nosepiece

· BX43-5RES

· U-5RE-2

· U-D6RE

Slide holder

· U-HLST-4

· U-HD-4

· U-HLDT-4

Intermediate attachment

· U-TRA

· U-CA

· U-DO3

Arm (or vertical illuminator)

· BX3-ARM

· BX3-URA*

· BX3-RFAS*

Lamp housing

· U-LHLEDC

· U-LS30-5

Stage

· U-SVRB-4

· U-SVRO

Condenser

· U-LC

· U-SC3

· U-AC2

5

BX43

2

} If you have not yet assembled the microscope, read section 10, “ASSEMBLY” (pages 33 to 37).

Interpupillary distance adjustment
scale (Page 22)

Diopter adjustment ring
(Page 22)

CONTROLS

Slide holder clamping lever
(Page 19)

Condenser height adjustment
knob (Page 12)

Aperture iris diaphragm ring
(Page 12)

Condenser centering screws
(Page 12)

Filter mount (Page 15)

Field iris diaphragm ring
(Page 12)

Coarse adjustment tension
adjustment ring (Page 18)

Allen screwdriver
holder

Coarse adjustment
knob (Page 18)

Y-axis knob (Page 20)

X-axis knob (Page 20)

6

Main switch (Page 10)
I : ON

: OFF

Pre-focusing lever
(Page 16)

Fine adjustment knob
(Page 16)

LIM indicator
ON: Lit (Green)

OFF: Extinguished

LIM setting switch

(Page 13)

LED brightness adjustment
knob (Page 13)

LIM ON-OFF switch

(Page 13)

7

BX43

Low Magnification Condenser
U-LC

Auxiliary lens mounting
tool (for BX53)

Auxiliary lens

* The auxiliary lens is mounted on the lamp adapter when the U-LC is used. (Page 34)

<<Modules for halogen lamp operation>>

LS30 Adapter
U-LS30ADP

Swing-out Condenser
U-SC3

Top lens

Top lens swing-out
knob

Aperture iris diaphragm
ring (Page 12)

Condenser mount
dovetail

Halogen Lamp Socket
U-LS30-5

Guide pin holes

Lamp cable

Brightness control
knob

Main switch
I : ON

: OFF

Guide pins

Power Supply Unit
TL4

Lamp socket connector

8

3

} When the LED lamp is used and the LIM is set, the LED brightness adjustment knob is defeated.
} When the halogen bulb is used, set the LBD filter in the filter mount on the base (or in the U-FC filter cassette).

Set the main switch to “ I ” (ON) and adjust
the brightness.

FLOW OF OBSERVATION

LED lamp Halogen bulb

@Main switch
2LED brightness

adjustment knob

(Controls Used) (Page)

3Main switch
|Brightness

*

adjustment knob

(P. 13)

Select the light path (trinocular tube).

Place the specimen on the stage.

Engage the 10X objective in the light path.

Bring the specimen in focus.

Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.

Adjust the aperture iris and field iris diaphragms.

Engage the desired objective in the light path
and bring the specimen in focus.

5Light path selector knob (P. 22)

6Slide holder (P. 19)
7X-/Y-axis knobs (P. 20)

8Revolving nosepiece

9Coarse/fine adjustment knobs (P. 17)

aBinocular tube (P. 21)
bDiopter adjustment ring (P. 21)
cCondenser height adjustment knob (P. 24)
dCondenser centering knob (P. 12)

eAperture iris diaphragm ring (P. 12)
fField iris diaphragm ring (P. 12)

8Revolving nosepiece

Insert the required filters.

Adjust the brightness.

Start observation.

* The LED brightness adjustment knob is defeated when the LIM is set (see page 34).

gFilter mount (P. 14)

2LED brightness

adjustment knob

|Brightness

*

adjustment knob

(P. 13)

9

BX43

b

e

g

8

6

d

a

(trinocular tube only)

5

c

1

7

f

2

} Copy the observation procedure pages on separate sheets and post it near your microscope.

9

4

3

10

4

SIMPLIFIED OBSERVATION PROCEDURE

4-1 Basic Operation (Until Observation of Specimen)

This section describes the basic operation of the microscope until the start of observation of a specimen. For the detailed

operating procedure of each control, please read the description page specified below.

Press the main switch of the microscope frame to “ I ” (ON).

1

1

Fig. 3

Rotate the knob to adjust the lamp brightness. (Details: Page 13)

2

2

Fig. 4

4

3

Fig. 5

Rotate the knob to lower the stage. (Details: Page 18)

3

Open the clamping lever of the slide holder and place the specimen

4

slide. (Details: Page 18)

11

BX43

Rotate the revolving nosepiece to engage the 10X objective in the

5

light path.

Rotate the coarse and fine adjustment knobs to bring the specimen

5

6

7

Fig. 6

6

into focus. (Details: Page 17)

Rotate the stage knob to adjust the observation position.

7

Now you can observe the magnified image of the specimen. To
improve the observation further, read section 4-2, “Microscope
Adjustments” below.

4-2 Microscope Adjustments (How to Improve the Observed Image)

Adjust the microscope as described below to improve the observed image.

1 Adjusting the Interpupillary Distance

While looking through the eyepieces, adjust for binocular vision until

1

the left and right fields of view coincide completely.
} Note your interpupillary distance so that it can be quickly duplicated.

1

Fig. 7

2

Fig. 8

2 Adjusting the Left and Right Focusing
(diopter adjustment)

Looking through the eyepiece without the diopter adjustment ring,

1

rotate the coarse and fine adjustment knobs to bring the specimen
into focus.

Looking through the eyepiece with the diopter adjustment ring, turn

2

only the diopter adjustment ring to focus on the specimen.
} If the diopter adjustment rings are provided on both the left and

right sides, see “Adjusting the Diopter” on page 21.

The diopter adjustment rings may be provided on either the eye-

pieces or the observation tube.

12

3 Adjusting the Centering

2

4 6

Place the specimen.

1

Rotate the revolving nosepiece to select the 10X objective.

2

Rotate the knobs to bring the specimen into focus.

3

Rotate the knob to raise the condenser to its upper limit.

4

Rotate the field iris diaphragm ring in the direction of the arrow so that the diaphragm image comes inside the field

5

of view.

Rotate the knobs to focus on the field iris diaphragm image (A in Fig. 9).

6

1

7

5

3

8 9

Turn the two condenser centering screws to move the iris diaphragm image to the center of the field of view (B in Fig. 9).

7

Gradually open the field iris diaphragm until its image is inscribed in the field of view (C in Fig. 9).

8

Open the field diaphragm slightly until its image circumscribes the field of view slightly (D in Fig. 9).

9

} Be careful not to rotate the condenser clamping screw, located behind the centering knobs, by mistake.
} When using a condenser with top lens such as the U-SC3, engage the top lens in the light path before proceeding to

the centering adjustment.

A B C D

Fig. 9 Movement of Field Iris Diaphragm Image

4 Adjusting the Contrast

Aperture iris
diaphragm image

Objective pupil

N. A. scale

Set the condenser aperture iris diaphragm ring so that the condenser NA scale position indicates 70% of the NA of the

1

objective in use. After this, observe the image and fine adjust the aperture iris diaphragm to the desired condition.

1

70-80%

30-20%

2

The image of the aperture iris diaphragm can be viewed by removing the eyepieces and looking into the observation

2

tube. Adjust so that the image looks approximately like that shown in the figure above.

13

BX43

5

USING THE CONTROLS

5-1 Base

1

Fig. 10

1 Adjusting the Brightness

1. When the LED lamp is used for observation, turn the LED brightness
adjustment knob @ clockwise to make illumination brighter.

2. When the halogen bulb is used for observation, adjust the brightness
adjustment knob 2 of the TL4 power supply unit.

(Fig. 10)

1

2

2

3

Fig. 11

4

Fig. 12

2 Setting the LIM Brightness

} LIM stands for Light Intensity Manager. It is effective when the BX43-5RES

revolving nosepiece with sensor and the U-LHLEDC LED lamp housing
are used.

} The desired brightness can be set per objective. Once the brightness for

the objectives is set, selecting an objective adjusts the LED brightness
automatically provided that the LIM ON-OFF switch @ is set to “ON” (which
is indicated by the lighting of the LIM indicator 2).

1. Press the LIM ON-OFF switch @ to “OFF” (so that the LIM indicator 2 is

extinguished).

2. Engage a low-power objective in the light path and bring the specimen

in focus.

3. Rotate the brightness adjustment knob 3 for optimum brightness for

observation.

4. Press the LIM setting switch | to enter the current brightness.

5. Set the optimum brightness for the mounted objectives by repeating the

above for each of them.

(Fig. 12)

To modify the LIM setting:

Engage the objective you want to set the new LIM setting in the light path,

set the LIM ON-OFF switch to “OFF,” and perform steps 3 and 4 above.

14

5

3

Fig. 13

Fig. 14

Fig. 15

2

4

3 Using the Filters (Halogen Bulb Operation Only)

} You can place a filter in the light path with either method.

· Place a filter on the filter mount on the base and engage in the light path.
(Page 14)

· Insert a filter in the U-FC filter cassette, attach it on the filter mount and
engage it in the light path by sliding the filter level. (Page 14)

Mounting a Single Filter (Fig. 13)

A filter with a diameter of 45 mm can be placed on the filter mount on

the base. If you need to place multiple filters, please purchase the U-FC
filter cassette.

CAUTION

1

CAUTION

Even when a filter cassette is used, a filter with thickness of no

more than 3 mm can still be placed on the filter mount.

Using the Filter Cassette (Figs. 14 to 17)

Loading Filters into Filter Cassette

} The filter cassette accommodates filters with a diameter of 45 mm and

thickness of 2.7 mm or less.

} The filter cassette has two filter levels on the right side and one on the

left side.

} When the filter cassette is used, the ECO mode cannot be used because

the human sensor is hidden by the filter cassette.

1. Move all filter levers to the OUT position except for the one belonging to
the slot into which the filter is to be inserted.

2. Slide lever @ to the IN position. Make sure that it clicks securely into
place. (Fig. 14)

3. Holding the lever in the position shown in Fig. 14, put the filter into the
cassette by inserting it in the direction indicated by the arrow.

4. Insert the other two filters in the same manner.

Mounting the Filter Cassette

1. Fully loosen the filter cassette clamping screw 2. (Fig. 15)

2. Align the key 3 on the bottom surface of the filter cassette with the
positioning slot | on the filter mount, then snap the filter cassette into
place from above.

3. Rotate the filter cassette to align its sides with the base. (Fig. 16)

4. Align the clamping screw 2 with the positioning hole 5 on the filter
mount, then tighten the screw to fasten the filter cassette.

When the filter cassette is installed, the condenser may hit it

when the stage is lowered. Therefore, exercise caution when
lowering the stage with the filter cassette installed.

15

BX43

Using the Filter Cassette (Fig. 17)

Usable Filters Applications

45LBD-IF* Color balancing filter

45-ND6, 45-ND25 Neutral density filter

Fig. 16

Fig. 17

6

45G-530, 43IF550-W45* Green

B&W contrast filters

45O-560 Orange

Up to three of the above filters can be loaded in the filter cassette. Moving

the levers 6 on the left and right sides of the cassette to the IN position
brings the corresponding filter into the light path.

* When an IF (interference) type filter is used, flare and ghost may be

observed. These phenomena may be reduced by adding ND filter(s) to
the IF filter.

} The LBD filter is not required when the LED lamp is used.

What is LBD filter?

The color of the sun at the noon of a clear day is called the daylight
color, which looks white to human eyes. On the other hand, the
halogen bulb used as the light source of this microscope does not
emit the daylight-color light. so the LBD filter is engaged in the light
path to convert the color of halogen bulb light into daylight. In general,
engaging the LBD filter makes it possible to observe specimens in
natural color tones.

16

5-2 Focusing Block

1

1 Focusing Controls

The focusing block consists of the controls listed in the following table,

which also describes the functions of the controls.

4

3

5

Fig. 18

1

Fig. 19

2

No. Name Function

Coarse adjustment

1

knob

Fine adjustment dial Fine adjusts the focus position.

2

Fine adjustment

3

knob

Pre-focusing lever Sets the upper limit of the stage position

4

Tension adjustment

5

ring

Moves the focus position widely.

Fine adjusts the focus position.
This knob can be attached to the fine
adjustment dial on either the left or right
side. (When the microscope is shipped,
it is attached to the right-side dial.)

(to prevent contact between the speci­men and objective).

Adjusts the force required to rotate the
coarse adjustment knob.

2 Adjusting the Focus

With both the coarse adjustment knob @ and fine adjustment knob 2,

rotating the knob in the direction of the arrow raises the stage (lets the
specimen approach the objective).

2

1

Fig. 20

17

BX43

3

1

4

Fig. 21

Fig. 22

2

3 Replacing the Fine Adjustment Knob

CAUTION

1

4

CAUTION

The fine adjustment knob has been attached on the right side

at the factory.

} The fine adjustment knob is designed detachable to prevent interference

with hand during manipulation of the X-and Y-axis knobs.

Usually attach the knob on the opposite side to the X- and Y-axis

knobs.

1. Using the Allen screwdriver, loosen the clamping screw @ and remove
the fine adjustment knob 2.

2. Remove the seal from the fine adjustment knob screw hole on the other
side and attach the knob by reversing the removal procedure.

3. Attach a provided seal on the screw hole | of the removed fine adjustment
knob 3.

} The fine adjustment dial 3 can be operated with your fingertip or finger

surface at the same time as manipulating the X- and Y-axis knobs.

Adjusting the Coarse Adjustment Knob Tension

Adjust the coarse adjustment knob tension using the tension

adjustment ring @.

The coarse adjustment knob tension is preadjusted for easy use. However,

if desired, you can change the tension using the tension adjustment ring
@. Turning the ring in the direction of the arrow increases tension, and
vice versa.

The tension is too low if the stage drops by itself of focus is quickly lost

after adjustment with the fine adjustment knob. In this case, turn the ring
Fig. 22 in the direction of the arrow to increase tension.

(Fig. 21)

(Fig. 22)

1

Fig. 23

5 Pre-focusing Lever

} The pre-focusing lever ensures that the objective does not come in contact

with the specimen and simplifies focusing.

After focusing on the specimen with the coarse adjustment knob, turn this

lever @ in the direction of the arrow and lock; the upper limit on coarse
adjustment movement is set at the locked position.

After changing a specimen, approximate refocusing is easily accomplis-

hed by rotating the coarse adjustment knob to reach the pre-focused
position. After this, all you have to do is make fine adjustments with the
fine adjustment knob.

} Stage movement with the fine adjustment knob is not locked.

CAUTION

When the pre-focusing lever is locked, a coarse movement stro-

ke is reduced due to the microscope’s mechanism, causing the
stage not to be able to be lowered to the lower limit. To lower
the stage to the lower limit, release the pre-focusing lever.

(Fig. 23)

18

5-3 Stage

2

Fig. 24

Fig. 25

1

1 Placing the Specimen

} The dimensions of the slide glass should be 26 x 76 mm with thickness of

0.9 to 1.2 mm, and the cover glass should have thickness of 0.17 mm.

} When observing very large specimens, remove the slide holder and place

the specimen directly on the stage.

Microscopy with Double-Slide Holder (Fig. 24)

1. Turn the coarse adjustment knob @ to lower the stage.

2. Open the spring-loaded curved finger 2 on the slide holder and place
one or two specimen slides on the stage from the front.

3. After placing the sides as far as they will go, gently release the curved
finger.

Microscopy with Single-Slide Holder (Fig. 25)

The specimen side can easily be placed by sliding it into the slide holder

from the front.

Examining a whole specimen

Use one of the thin slide holders listed below that cause less interference

with the objectives*.

· U-HRD-4 · U-HLD-4 · U-HLS-4

* The applicable objectives are ones with 40X or less magnification (except

Apo series).

Using an Oil Immersion Condenser

Use the optional U-SVRO (right-hand knob) or USVLO (left-hand knob)

slotted stage which prevents close contact between the specimen and
stage.

1

Fig. 26

2 Using the Auxiliary Slide Holder

Applicable revolving nosepieces: U-D7RE, U-D7RES, U-P6RE,
U-D6BDRE, U-P5BDRE

} When a biological slide glass specimen is placed on the U-HLDT4/U-

HRDT4 two-slide holder (thick type), the slide holder may interfere with
the objective when the following conditions are combined;

· The objective in use has a short WD (Working Distance);

· The sspecimen being observed is the specimen placed on the rear
side of the side holder, and;

· The stage is in a rotated position.

If the slide holder contacts the objective, set the provided auxiliary slide

holder @ as shown in the figure and observe only the specimen on the
front side of the slide holder.

19

BX43

2

4
3

1

Fig. 27

3 Adjusting the X- and Y-Axis Knob Tension

1. Hold the X-axis knob @ and slide up the Y-axis knob 2 up to expose
the adjustment knobs.

2. Turning the X-axis adjustment knob 3 or Y-axis adjustment knob |
clockwise (in the direction of the arrow) increases the tension and
counterclockwise decreases it.

CAUTION

If the tension is adjusted to tight, a creaking sound may be heard

during stage travel, and the stage stopping accuracy may be
imperiled.

} After long hours of use, the stage guide may be deviated and the stage

travel range may be decreased.

However, this is not malfunction and can be corrected easily as described

below.

[Treatment]

Horizontal direction: Hold the specimen holder and move the stage guide

to the left and right so that it hits the stoppers.

Vertical direction: Hold the upper stage and move it to the front and rear

so that it hits the stoppers.

Stage Feed Knob Rubber Caps (Optional)

} When the X- and Y-axis knobs are fitted with the rubber caps, the knobs

can be adjusted without slipping and fine adjustment is possible by
holding the knobs with a very light force. The knob rubber caps also
reduce fatigue after long hours of operation.

The U-SHGT thick type (thickness 5 mm) and U-SHG thin type (thickness

2 mm) knob rubbers are available.

(Fig. 27)

To attach the knob rubbers:

First fit the larger knob rubber to the Y-axis (upper) knob from below it,

then fit the smaller knob rubber to the X-axis (lower) knob from below it.

20

1

Fig. 28

4 Rotating the Stage

1. Slightly loosen the stage clamping screw @.

2. The stage can be rotated both clockwise and counterclockwise by the
stage clamping screw.

CAUTION

A click may be heard and felt during rotation. However, this is

due to the construction of the substage and does not indicate
a malfunction.

} The angle of rotation varies depending on the positions of the X- and

Y-axis knobs.

Angle of Rotation

Clockwise Counterclockwise

(Fig. 28)

Fig. 29

2

1

Right hand knobs

Left hand knobs 20° 230°

5 Adjusting the Stage Height

} By lowering the position of the substage, the microscope will accommo-

date specimens with maximum height of 35 mm. This is useful when
observing metallurgical specimens and other thick objects.

1. Lower the stage to the lower limit, then remove the stage from the
microscope.

2. Using the Allen screwdriver, loosen the substage bracket clamping screw
@ and remove the substage.

3. Turn the coarse adjustment knob and raise the focusing block 3 to where
the stopper screw 2 on the arm becomes visible.

4. Using the Allen screwdriver, loosen and remove the upper stopper screw

2.

3

5. Reattach substage bracket and stage.

} Store the removed stopper screw 2 in a safe place so that it will not be

lost, if needed again.

230° 20°

(Figs. 29 & 30)

Fig. 30

21

BX43

5-4 Observation Tube

1

Fig. 31

2

1 Adjusting the Diopter

When diopter adjustment rings are provided on both the left and right, it
is possible to adjust the optimum vision for both the left and right eyes as
described below. The diopter adjustment rings may be provided either on
the eyepieces or the observation tube, and there is no problem even when
the rings are not provided on both of them.
The diopter adjustment ensures parfocality from low to high magnifications
and makes it possible to avoid defocusing even when the objective is
switched.

1. Set the diopter adjustment rings on both sides to scale “0”.

2. Engage a high-power objective (40X or so) in the light path, look into
the right eyepiece with your right eye, and rotate the coarse and fine
adjustment knobs to bring the specimen into focus.

} Do not use an oil-immersion objective.

3. Engage a low-power objective (10X or so) in the light path, rotate the right
diopter adjustment ring @ to bring the specimen into focus. Do not touch
the coarse and fine adjustment knobs during the focusing.

4. Looking into the left eyepiece with your left eye, rotate the left diopter
adjustment ring 2 to bring the specimen in focus.

} The above procedure adjusts the diopter with reference to the right eye,

but it is also possible to adjust with reference to the left eye. In this case,
read the above procedure by inverting “right” and “left.”

} If an eyepiece equipped with a diopter adjustment ring is inserted into

the side equipped with another diopter adjustment ring of the observation
tube, perform the above procedure without moving the diopter adjustment
ring of the observation tube from the “0” position.

Fig. 32

2 Using the Eye Shades

When Wearing Eyeglasses

Use with the eye shades in the normal, folded-down position. This will

prevent the eyeglasses from being scratched.

When Not Wearing Eyeglasses

Extend the folded eye shades in the direction of the arrow to prevent

extraneous light from entering between the eyepieces and eyes.

(Fig. 32)

22

2

Fig. 33

1

3 Using the Eyepiece Micrometer Disk

When the WHN10X-H (WHN10X) eyepieces are used, an eyepiece

micrometer disk can be inserted in one of them. When the eyepiece
does not have a diopter adjustment mechanism, however, it is hard to
focus on the micrometer disk if the operator has poor eyesight. Should
that be the case, adjust the focus with eyeglasses on.

Use 24 mm dia. x 1.5 mm thick micrometer disks.
Following Fig. 33, turn the built-in micrometer-mounting frame 2 coun-

terclockwise to remove it from the eyepiece and place a micrometer
disk @.

Screw the micrometer mounting frame back into the eyepiece sleeve.
} Be careful not to touch the lens surface with your finger.
} The micrometer-mounting frame may be tight for certain micrometer disks.

In this case, turn the frame slowly but with a stronger force than usual.

(Fig. 33)

Fig. 34

1

4 Selecting the Light Path of the Trinocular Tube

Slide the light path selector knob @ to select the desired light path.

Trinocular tube

U-TR30-2

U-SWTR-3

U-TR30NIR*

U-TTR2

* With the infrared trinocular tube, infrared observation up to 1000 nm is

possible. For details, consult your Olympus representative.

} The light path selector knob is located on the right side of the observation

tube, but it can be removed and attached on the left side when the U-TTR
is used.

After changing the knob position, be sure to tighten the knob firmly with

a coin.

100% for bino­cular eyepieces

50% for binocu­lar eyepieces,
50% for camera

Light path selector knob position

Pushed in Middle position Pulled out

20% for binocu­lar eyepieces,
80% for camera

50% for binocu­lar eyepieces,
50% for camera

100% for binocu­lar eyepieces

100% for camera

23

BX43

5° to 35°

Fig. 35

Fig. 36

1

5 Adjusting the Tilt

With the U-TBI-3 (Fig. 35)

} Adjust the height and tilt of the observation tube to obtain the most

comfortable viewing position.

U-TBI-3 5° to 35°

U-TBI-3-CLT 5° to 35°

U-ETBI 0° to 25°

U-TTBI 0° to 25°

U-TTR2 5° to 35°

U-TTLBI 0° to 27°

Holding the binocular section @ with both hands, raise or lower it to the

desired position.

CAUTION

Never attempt to force the binocular section past the upper or

lower stop position. Applying excessive force could destroy the
limiting mechanism.

} The U-TBI-3/U-TBI-3-CLT can be used in combination with only one

intermediate attachment.

} If you need photography using the U-TBI-3/U-TBI-3-CLT, use the U-TRU

trinocular tube.

(Figs. 35 to 38)

2

Fig. 37

Fig. 38

With the U-ETBI/U-TTBI (Fig. 36)

The U-ETBI and U-TTBI are ergonomic observation tubes with normal

field, capable of the adjustments of the positioning angle (0° to 25°) as
well as front-rear positioning (by 45 mm) of the eyepieces. The U-ETBI is
the erect image model and the U-TTBI is the inverted image model, and
both models are of the same size.

CAUTION

The intermediate attachments that can be combined with the

U-TTBI are limited. For details, please contact Olympus.

With the U-TTLBI (Fig. 37)

The U-TTLBI is a Tilting, Telescopic, Lifting binocular tube with the following

adjustment capabilities.

· Eyepiece angle adjustment (0° to 27°)

· Eyepiece up-down position adjustment (45 mm)

· Observation tube front-rear position adjustment (55 mm)

} When adjusting the front-rear position of the observation tube, hold the

base of the observation tube as shown in Fig. 38.

} When storing the microscope, increase the angle of the eyepieces so as

to prevent the eyepieces from dropping.

} To adjust the up-down position of the eyepiece, turn the dial 2.

24

2

1

Fig. 39

6 Using Eyepieces Incorporating a Micrometer

} When the eyepieces in use incorporate a micrometer, the accuracy of

the left-right focusing adjustment (diopter adjustment) can be improved
further.

1. Looking into the right eyepiece with your right eye, turn the top of the
eyepiece @ so that the micrometer in the field of view looks sharpest
(Fig. 39).

2. Looking into the right eyepiece, rotate the coarse and fine adjustment
knobs to bring the specimen and micrometer (cross lines, etc.) in focus.

3. Looking into the left eyepiece with your left eye, rotate the diopter
adjustment ring 2 to bring the specimen into focus.

5-5 Condenser

For how to adjust the centering of the condenser, see page 12.

1 Compatibility of Objectives and Condensers

Objective
Magnification

1.25X

2X

4X

10-60X

100X

Low

magnification

U-LC

Applicable

(FN 22)**

Abbe

U-AC2

Applicable

(FN 22)

Applicable

(FN 26.5)

Achromat/aplanat

Condenser

U-AAC

Applicable

(FN 26.5)

Swing-out

U-SC3

Applicable

(FN 22)

Applicable (FN 26.5)
by moving top lens
out of the light path*
(FN 26.5)

Engage top lens in
light path (FN 26.5)

Ultralow

magnification

U-ULC-2

Applicable

(FN 26.5)

* When using the U-SC3 swing-out condenser together with a 1.25X to 4X objective, fully open the condenser aperture

iris diaphragm and use the field iris diaphragm in the base as aperture diaphragm. With the 1.25X to 2X objectives, the

surroundings of the field of view may become dark.
} To obtain better illumination in camera recording using a 1.25X to 4X objective, use of the U-ULC-2 is recommended.
**When the U-LC is used with an objective with an NA of 1.0 or more, the NA of the lighting becomes insufficient.
} When the U-LC is used, it is required to attach the auxiliary lens (page 34).

25

BX43

5-6 Immersion Objectives

CAUTION

Be sure to use the provided Olympus Immersion oil.

1 Using Immersion Objectives

1. Focus on the specimen with objectives in the order of lower-power to
higher-power ones.

2. Before engaging the immersion objective, place a drop of provided
immersion oil onto the specimen at the area to be observed.

3. Turn the revolving nosepiece to engage the immersion objective, then
focus using the fine adjustment knob.

CAUTION

Fig. 40

CAUTION

Since air bubbles in the oil will affect the image quality, make

sure that the oil is free of bubbles.

a. To check for bubbles, remove the eyepiece and fully open the field and

aperture iris diaphragms, then look at the exit pupil of the objective inside
the observation tube. (The pupil should appear round and bright.)

b. To remove bubbles, turn the revolving nosepiece to repeatedly defocus

and refocus the oil immersion objective.

} If the condenser engraving shows a numerical aperture (NA) of 1.0 or

higher, the number applies only when oil is applied between the slide
glass and the top surface of the condenser. When oil is not present, the
NA is about 0.9.

4. After use, remove immersion oil from the objective front lens by wiping
with gauze slightly moistened with absolute alcohol.

Caution in use of immersion oil

If immersion oil enters your eyes or contacts with your skin, imme-

diately take the following treatment.

Eyes: Rinse with fresh water (for 15 minutes or more).
Skin: Rinse with water and soap.

If the appearance of the eyes or skin is altered or pain persists,

immediately see your doctor.

(Fig. 40)

5-7 Objectives with Correction Collar

} If the thickness scale setting of the objective with collection collar does

not match the cover glass thickness, the objective cannot manifest the full
performance. To prevent this, be sure to perform the following adjustment
when using an objective with collection collar.

Adjustment Procedure

1

Fig. 41

· If the cover glass thickness is known, set the correction collar @ to that
scale value. (Fig. 41)

· If the cover glass thickness is unknown, adjust the correction collar @
and fine adjustment knob alternately until the positioning with the highest
contrast is obtained.

} Be careful not to touch the correction collar @ when turning the revolving

nosepiece.

26

6

} Use a trinocular observation tube such as the U-TR30-2 or U-SWTR-2 when shooting the video or digital camera picture

The trinocular tube accepts a camera adapter (certain TV adapters necessitate a camera mount adapter).
} Be sure to adjust the parfocality before using a camera adapter. Otherwise, the focusing of the camera image will not

For details, refer to the instruction manuals for these accessories.

CAMERA RECORDING

of the microscope images.

match that of the image observed through eyepieces. For the parfocality adjustment method, refer to the instruction
manual for the camera adapter in use.

1 System Chart

TV camera/digital camera

Camera mount adapter

C-mount camera adapter

Camera adapter

Trinocular observation tube
U-TR30-2
U-SWTR-3
U-TTR2, etc.

2 Selecting the Camera Adapter Magnification

The image pickup area is determined by the size of the image sensor used in the TV camera or digital camera and the

magnification of the camera adapter.

The following illustrations show the image pickup areas of camera adapters when the SWH10X-H eyepieces with a FN

of 26.5 are used (the FN of the WHN10X eyepieces is 22).

1-inch image sensor

2/3-inch image sensor

1/2-inch image sensor

WHN10X: FN22

SWH10X-H: FN 26.5

When a 0.5X camera adapter is used When a 1X camera adapter is used

27

BX43

7

Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur,

please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire
list, please contact your local Olympus representative for assistance.

Bulb does not light.

Bulb is blown immediately. Halogen The bulb is not a designated

Field of view is dark.

TROUBLESHOOTING GUIDE

Problem Lamp Cause Remedy Page

LED Power is not ON. Set the main switch to “ I ” (ON). 10

Common Power cord is unplugged. Plug power cord into the power

outlet.

Common Connectors are connected

improperly.

Halogen Bulb is not attached. Attach a halogen bulb. 34

Halogen Bulb is burned out. Replace bulb. 34

Halogen The TL4 is not ON. Set the main switch of the TL4 to

one.

LED The LIM setting is inappropriate. Set LIM again. 13

Common Aperture and field iris diaphragms

are not opened wide enough.

Common Light path selector knob is set to

the

position.

Common Con d e nser i s n o t prope r l y

centered.

Common Condenser height is too low. Adjust correctly. 24

Connect connectors securely.

“ I ” (ON).
Replace bulb with a designated

one.

Adjust them to proper sizes.

Set the knob to the
position.

Center the condenser.

or

37

37

37

1

12

22

12

Brightness cannot be varied.

Field of view is obscured or not
evenly illuminated.

Halogen ND filter is engaged. Disengage the ND filters. 15

LED LIM is not ON. Switch the LIM function OFF. 13

Halogen LED brightness adjustment knob

is rotated.

Common

Common Condenser height is too low. Adjust correctly. 24

Common Light path selector knob is set in

Common Ob je c ti v e is not co r re c tl y

Common Revolv i n g no sepiece is not

Common

Common An objective that falls outside

Common

Common Field iris diaphragm is stopped

Halogen Bulb is not mounted correctly. Push the pins of halogen bulb all

Cond e n s er is no t p r o perl y
centered.

an intermediate position.

engaged/

attached properly.
Condenser is not at t a c h e d

properly.

of the condenser’s illumination
range is used.

Cond e n s er is no t p r o perl y
centered.

down too far.

Use the brightness adjustment
knob of the TL4.

Center the condenser.

Set to the desired click position.

Make sure that the revolving
nosepiece clicks properly into
place.

Push the slide dovetail all the way
until it is stopped.

Re-attach it .

Use a condenser to match the
purpose. 24

Center the condenser.

Open the field iris diaphragm until
it circumscribes the field.

the way until the stop position.

37

12

22

12

35

36

12

12

34

28

Problem Lamp Cause Remedy Page

Dirt or dust is visible in the field
of view.

Visibility is poor

· Image is not poor.

· Contrast is poor.

· Details are indistinct.

· Image glares.

One side of image is blurred. Common Objective is not correctly engaged

Image appears to waver. Common Rev o l v ing nosepiece is not

Coarse adjustment knob is hard
to turn.

Image cannot be focused:

· Objective drifts down by itself.

· Fine adjustment knob slips.

Common Dirt/dust on the eyepieces. Clean thoroughly.

Common Dirt or dust on condenser top

lens.

Common Dirt/dust on the specimen.

Common A non-UI S/UIS 2 obje ctive is

used.

Common Condenser height is too low. Adjust correctly. 24

Common Aperture iris diaphragm is not

opened enough.

Common Revolving nosepiece is not at-

tached properly.

Common Correction collar on correction

collar-equipped objective is not
properly adjusted.

Common Front lens of objective is dirty. Clean objective. 3

Common Immersion oil is not being used

with an oil immersion objective.

Common Immersion oil contains bubbles. Remove the bubbles. 25

Common Recommended immersion oil is

not used.

Common Dirt/dust on specimen. Clean it.

Common Dirt/dust on condenser top lens.

in light path.

Common Stage is not correctly mounted. Re-attach it. 18

Common Revolving nosepiece is not at-

tached properly.

Common Specimen is mounted upside

down.

attached properly.

Common Objective is not correctly engaged

in light path.

Common Con d e nser i s n o t prope r l y

centered.

Common Tension ad j u s t m e n t ring is

tightened excessively.

Common You are trying to raise stage

without coarse adjustment knob
while pre-focusing lever is kept
locked.

Common Tension adjustment ring is too

loose.

Use only UIS2/UIS series objecti­ves with this microscope.

Open aperture iris diaphragm.

Push the slide dovetail all the way
until it is stopped.

While focusing, turn the correction
collar to find the best position. 25

Use immersion oil.

Use an Olympus-designated
immersion oil.

Make sure that revolving nosepiece
clicks into place correctly.

Push the slide dovetail all the way
until it is stopped.

Mount specimen correctly.

Push the slide dovetail all the way
until it is stopped.

Make sure that revolving nosepie­ce clicks into place correctly.

Center the condenser.

Loose ring.

Unlock pre-focusing lever.

Tighten ring.

32

12

35

25

25

12

35

18

35

12

12

16

17

16

3

3

29

BX43

Problem Lamp Cause Remedy Page

Coarse adjustment will not go all
the way up.

Coarse adjustment will not go all
the way down.

Objective makes contact with
sp e c i m e n b ef o re foc u s i s
obtained.

Field of view of one eye does not
match that of the other.

Image shifts when you touch
stage.

X- and Y-axis knobs are too tight
or too loose.

Stroke has reduced. Common Stage guide is deviated. Correct deviation as described. 19

Common Pre-focusing lever is locked at a

low position.

Common Condenser holder is too low. Raise condenser holder.

Common Specimen is mounted upside

down.

Common

Common Diopter adjustment is incorrect. Adjust diopter. 21

Common Different eyepieces are used on

Common Your view is not accustomed to

Common Stage is not properly mounted. Clamp stage.

Common Tension of X- and Y-axis knobs is

Interpupillary distance is incorrect.

left and right.

microscope observation.

too high or too low.

Unlock pre-focusing lever.

Mount specimen correctly.

Adjust interpupillary distance. 11

Change one eyepiece to match
the other so that both sides are
the same type.

Upon looking into eyepieces,
try looking at overall field before
concentrating on specimen ran­ge. You may also find it helpful
to look up and into distance for
a moment before looking back
into microscope.

Adjust tension.

17

36

18

32

21

18

19

30

8

1. Optical system UIS2 (UIS) optical system (featuring infinity correction)

2. Illumination Built-in transmitted Koehler illumination

3. Focusing mechanism Stage up-down movement by roller guide (rack & pinion)

4. Revolving nosepiece Type

5. Observation tube Type

6. BX stage Type

SPECIFICATIONS

Item Specification

FN (Field Number): 26.5 (super-widefield compatible)
LED illuminator (continuously variable): U-LHLEDC
Power consumption: 0.5 A

Optional 6V 30W halogen bulb (continuously variable)
Power supply unit TL4: 100-120/220-240 V $ , 0.85/0.45 A, 50/60 Hz.
Power consumption: 45 W

Stroke per rotation: 0.1 mm (fine), 17.8 mm (coarse)
Full stroke range: 25 mm
Upper coarse adjustment limit stopper
Tension adjustment on coarse focus adjustment knob.

BX43-5RES U-D6RE

5-position revolving nosepiece with

readout function

Attachable
module

U-BI30-2 U-TBI-3/U-TBI3-CLI U-TR30-2 U-SWTR-3

Widefield binocular

Field No. 22 26.5

Tube
inclination

Interpupillary
distance
adjustment

Light path
selection Not available

30°

U-SVRB-4 U-SVLB-4

Transmitted light DIC slider

Transmitted light analyzer

Widefield, tilting

binocular

5-35°

(continuously variable)

50-76 mm

Universal 6-position revolving nosepiece

Widefield,

trinocular

3 steps: @ Binocular 100%

2 Bi 100%, Cam 80%
3 Camera 100%

Super- widefield,

trinocular

30°

Coaxial knobs on bottom right. Coaxial knobs on bottom left.

Rectangular ceramic-coated, wire-driven stage

Size 156 mm (D) x 191 mm (W)

Movement
mechanism

Slide holder
(single-slide
holder)

Slide holder
(double-slide
holder)

X- and Y-axis knob with adjustable tension. Movement range: 52 mm in vertical (Y)
direction, 76 mm in horizontal (X) direction.

U-HLST-4 (Thick type)

U-HLS-4 (Thin type)

U-HLDT-4 (Left-opening, thick type)

U-HLD-4 (Left-opening, thin type)

U-HRDT-4 (Right-opening, thick type)

U-HRD-4(Right-opening, thin type)

31

BX43

Item Specification

7. Condenser Type

Max. N.A . 0.75 1.10 0.9 1.40

Applicable
objective
powers

8. Operating
environment

9. Rating

· Indoor use.

· Altitude: Max. 2000 meters

· Ambient temperature: 5° to 40°C (41° to 104°F)

· Maximum relative humidity: 80% for temperatures up to 31°C (88°F), decreasing linearly through
70% at 34°C (93°F), 60% at 37°C (99°F), to 50% relative humidity at 40°C (104°F).

· Supply voltage fluctuations: ±10%

· Pollution degree: 2 (in accordance with IEC60664-1)

· Installation/Overvoltage category: II (in accordance with IEC60664-1)

Input rating: 5 V, 2.5 A
AC adapter SA115B-05V: 100V-240Vac 50/60 Hz, 0.4 A

U-LC U-AC2 U-SC3 U-AAC

Low magnification Abbe Swing-out Achromat/ Aplanat

2X - 60X
(Widefield: FN 22)

4X
(Widefield: FN 22)
10X - 100X
(Super widefield:
FN 26.5)

1.25X
(Widefield: FN 22)
2X - 100X
(Super widefield:
FN 26.5)

10X - 100X
(Super widefield:
FN 26.5)

32

9

OPTICAL CHARACTERISTICS (UIS2 Series)

UIS series objectives not listed here can also be combined with this microscope.

The following table shows the optical characteristics of com-

binations of eyepieces and objectives. The figure on the right
shows the performance data engraved on the objectives.

NOTE

Refer to the latest catalogue or consult your local Olympus

representative for the updated information on the eyepieces
and objectives that can be combined with this microscope.

Optical character

Objective
Series Marking

PLN (PH)
Plan
Achromat
(FN 22)

PH: Phase
contrast

UPLFLN (PH)
Plan Semi
Apochromat
(FN 26.5)

PH: Phase
contrast

UPLSAPO
Plan
Apochromat
(FN 26.5)

PLAPON
Plan
Apochromat
(FN 26.5)

UApoN
Apochromat
(FN 22)

PlanN (Ph) 2X

UPlanFLN
(Ph)

UPlanSApo 4X

PlanApoN

UApoN 20XW340

Magnifi-

cation

4X
10X (Ph)
20X (Ph)
40X (Ph)
50XOI

100XO (Ph)

4X (Ph)
10X2 (Ph)
20X (Ph)
40X (Ph)
40XO
60X
60XOI

(Ph)
100XO2
100XOI2

10X2
20X
20XO
40X2
60XW
60XO

100XO

1.25X
2X
60XO

40XW340

40XO340

0.06

0.1

0.25

0.4

0.65

1.25

0.13

0.3

0.5

0.75

1.3

0.9

0.65-1.25

1.30

(Ph)

0.6-1.30

0.16

0.4

0.75

0.85

0.95

1.2

1.35

1.4

0.04

0.08

1.42

0.7

1.15

1.35

N.A.

0.5-0.9

W.D.

(mm)

5.8

18.5

10.6

1.2

0.6

0.2

0.15

17.0

10.0

2.1

0.51

0.2

0.2

0.12

0.2

0.2

13.0

3.1

0.6

0.17

0.18

0.28

0.15

0.13

5.0

6.2

0.15

0.35

0.25

0.1

Mechanical tube
length

Cover

glass
thick-

ness

(mm)

–
–
–

0.17

0.17

0.17
–

–
–

0.17

0.17

0.17

0.17

0.17

0.17

0.17

–

0.17

0.17
–

0.17

0.17

0.17

0.17

–
–

0.17

0.17

0.13-0.25

0.17

Magnification

Color band

Reso-

lution

(µm)

5.59

3.36

1.34

0.84

0.52

0.37

0.27

2.58

1.12

0.67

0.45

0.26

0.37

0.27

0.26

0.26

2.10

0.84

0.45

0.39

0.35

0.28

0.25

0.24

8.39

4.19

0.24

0.48

0.29

0.25

Total mag.

20X

40X
100X
200X
400X
500X

1000X

40X
100X
200X
400X
400X
600X
600X

1000X
1000X

40X
100X
200X
200X
400X
600X
600X

1000X

12.5X
20X
600X

200X

400X

400X

Eyepiece

WHN10X (FN22)

Depth of

focus

(µm)

560.1

175.0

28.0

9.27

3.04

1.7

0.69

127.2

22.4

7.0

2.52

1.27

1.5

0.98

0.66

0.66

99.6

15.9

4.29

3.5

1.9

1.03

0.89

0.59

1326.8

398.3

0.83

4.08

1.29

1.2

Objective type

Number of aperture

Oil: Oil immersion
Iris: Iris diaphragm

Field number (FN)

Cover glass thickness

Field of

view

11.0

5.5

2.2

1.1

0.55

0.44

0.22

5.5

2.2

1.1

0.55

0.55

0.37

0.37

0.22

0.22

5.5

2.2

1.1

1.1

0.55

0.37

0.37

0.22

17.6

11.0

0.37

1.1

0.55

0.55

Remark

Oil immersion/Iris
Oil immersion

Oil immersion
Correction collar
Oil immersion/Iris
Oil immersion
Oil immersion/Iris

Oil immersion
Correction collar
Water immersion
Oil immersion
Oil immersion

Oil immersion

Water immersion
Water immersion/

Correction collar
Oil immersion

6

1

9

7

8

5

10

2

11

4

3

4

4

4

1212

33

BX43

10

ASSEMBLY

10-1 Assembly Diagram

The diagram below shows the sequence of assembly of the various modules. The numbers indicate the order of

assembly.
Assembly steps enclosed in
The module numbers shown in the following diagram are merely the typical examples. For the modules with which the

module numbers are not given, please consult your Olympus representative or the catalogues.

CAUTION

Slide holder
U-HLDT-4
U-HRDT-4, etc.

When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching

any parts or touching glass surfaces.

} Most assembly operations are possible by using the Allen screwdriver (
However, the assembly of the reflected light illuminator requires the use of the provided Allen wrench (

the internal screws (to ensure the performance, we recommend that you have your Olympus representative assemble

or remove this module).

Eyepieces
WHN10X (FN 22)
WHN10X-H (FN 22)
SWH10X-H (FN 26.5)
MICRO SWH10X (FN 26.5), etc.

Observation tube

Revolving nosepiece
BX43-5RES
U-5RE-2
U-D6RE
U-D7RES, etc.

UIS2 series
objective

clamping screw

Revolving nosepiece
clamping screw

Slide holder
clamping knobs

will be detailed on the subsequent pages.

Observation tube
U-BI30-2
U-TTLBI
U-TR30-2, etc.

Arm
BX3-ARM

Intermediate attachment
U-EPA2
U-DO3
U-TRU, etc.

) provided with the microscope.

Reflected light
mercury/xenon
lamp housing

Vertical illuminator
BX3-URA
BX3-RFAS, BX3-RFAA, etc.

Halogen bulb
6W30WHAL

Lamp housing adapter
U-LS30ADP

) for clamping

Stage clamping
knob

Stage feed knob
rubber caps
U-SHG
U-SHGT

Stage
U-SVRB-4
U-SVLB-4, etc.

Condenser
U-LC
U-AC2
U-SC3
U-UCD8 (T2), etc.

Condenser
clamping knob

AC adapter for
microscope

Microscope frame
BX43F

Power cord

LED lamp housing
U-LHLEDC

Auxiliary lens for
U-LC

Power supply unit
TL4

Lamp socket
U-LS30-5

Lamp cable

Power cord

34

10-2 Detailed Assembly Procedures

1

1

Fig. 42

Fig. 43

5

2

Attaching the Auxiliary Lens for the U-LC

2

Condenser

} It is not necessary to attach the auxiliary lens if the condenser in use is

other than the U-LC.

1. Attach the auxiliary lens @ provided with the condenser into the tip of the
LED lamp housing C (U-LHLEDC) 2 or lamp housing adapter (U-LS30ADP)
by screwing the lens in the direction of the arrow.

CAUTION

3 Attaching the LED Lamp Housing C

3
4

2

Be careful not to touch the lens with your finger.

1. Fit the LED lamp housing @ into the mount hole on the rear of the
microscope by aligning the clamping screw 2 and screw hole 3.

2. Using the Allen screwdriver, tighten the clamping screw 2.

3. Insert the connector | of the lamp housing into the connector 5 on the
microscope.

(Fig. 42)

(Fig. 43)

1

Fig. 44

4 Attaching the Halogen Lamp Socket

Installing the U-LS30ADP LS30 Adapter (Fig. 44)

1. Peel off the backing of double-side adhesive tape on the cable holder
@ and attach the holder on the specified position.

The area (enclosed with broken lines) on the outer side is for use in

attaching the cable holder for another option.

2. Attach the adapter in the same way as steps 1 and 2 in the LED lamp
housing C attaching procedure described above.

(Fig. 44)

35

BX43

Installing the Halogen Bulb (Fig. 45)

Use only the designated bulb 6V30WHAL (PHILIPS 5761).

2

3

7

4

Fig. 45

Fig. 46

5

6

1. Holding the bulb 2 a piece of gauze, insert the bulb pins 3 straight and
fully into the pin holes | on the lamp socket.

CAUTION

CAUTION

To prevent reduced bulb life or cracking, do not touch the bulb

with bare hands. If fingerprints are accidentally left on the bulb,
wipe the bulb with a soft cloth.

2. Insert the lamp cable 5 in the direction shown in the figure 46.

3. Aligning the guide pins 6 with the guide pin holes † at the rear of the
microscope frame, gently push the lamp socket all the way into place.

Caution for Bulb Replacement During Use or Right After Use

The bulb and the lamp socket are areas near these will be

extremely hot during and right after use.

Set the main switch to “

from the wall outlet, then allow the old bulb and lamp socket
to cool before replacing the bulb with a new of the designated
type.

4 Pass the lamp cable through the cable holder @ (Fig. 44).

” (OFF), disconnect the power cord

2

3

Fig. 47

Fig. 48

1

8
9

Connecting the Lamp Cable (Fig. 47)

Connect the cable connector 8 to the connector 9 of the TL4 power

supply unit.

8 Attaching the Revolving Nosepiece

1. Rotate the coarse adjustment knob to lower the stage.

2. Loosen the revolving nosepiece clamping screw @ slightly. Do not loosen
it too much because doing so makes it impossible to attach the revolving
nosepiece.

3. Hold the revolving nosepiece so that the mount dovetail 2 is horizontal
and insert it all the way into the dovetail groove of the microscope arm

3.

4. Tighten the revolving nosepiece clamping screw @.

} When attaching or detaching a revolving nosepiece with a cable, be sure

to disconnect the cable from the revolving nosepiece in advance.

(Fig. 48)

36

Fig. 49

4

Use only coded 5 position nosepiece for BX43 (BX43-5RES)

} Distribute its cable | toward the rear panel by passing it below the arm,

and connect the cable to the connector no the rear panel.

} As shown in Fig. 49, connect to the connector by letting the cable into

the cable holder (attached with BX43-5RES) attached to the rear panel.

Fig. 50

Fig. 51

3

1

1

2

9 Attaching the Condenser

2

1. Turn the coarse adjustment knob @ to raise the stage to its upper limit.

2. Turn the condenser height adjustment knob 2 to lower the condenser
holder to its lowest position.

3. Fully loosen the condenser clamping knob 3.

4. Position the condenser so that the aperture scale is located in front.

Gently slide the condenser along the dovetail all the way until it is

stopped.

} When attaching the U-SC3 swing-out condenser, align the positioning pin

at the back of the condenser with the groove in the condenser sleeve.

CAUTION

· When attaching a swing-out condenser with a positioning pin at
the back such as the U-SC3, attach by aligning the positioning
pin with the groove in the condenser sleeve.

· When attaching a condenser with swing-out top lens, swing out
the top lens out of the way before inserting the condenser.

5. Tighten the condenser clamping knob and gently raise the condenser
holder to its highest position.

10 Attaching the Eyepieces

Gently insert the eyepieces all the way into the eyepiece sleeves.

CAUTION

· When using the U-BI30-2 binocular tube, eyepieces with a po­sitioning pin cannot be attached because the U-BI30-2 does
not have the positioning notch.

· When using an eyepiece with micrometer, attach it to the right­hand eyepiece sleeve.

When doing so, make sure that the eyepiece positioning pin @

fits into the notch 2 on the eyepiece sleeve.

· The super-widefield trinocular observation tube is equipped with
a positioning notch on both the left and right eyepiece sleeves.
Make sure that the positioning pins on both eyepieces fits into
the respective notches.

(Fig. 50)

(Fig. 51)

37

BX43

11 Attaching the AC Adapter

Fig. 52

1

Fig. 53

2

CAUTION

2

1

CAUTION

· Cables and cords are vulnerable when bent or twisted. Never
subject them to excessive force.

· Make sure that the main switch is set to “
connecting the power cord (Fig. 52).

1. Insert the connector @ of the AC adapter firmly into the connector 2 on
the microscope.

Always use the designated AC adapter. Using a non-designated

AC adapter may cause malfunction.

12 Attaching the Power Cord

CAUTION

CAUTION

CAUTION

Always use the power cord provided by Olympus. If no power

cord is provided with the microscope, please select the proper
power cord by referring to section “PROPER SELECTION OF THE
POWER SUPPLY CORD” at the end of this instruction manual.

Operation Using the AC Adapter (Fig. 53)

1. Insert the connector @ of the power cord firmly into the input connector

2 of the AC adapter (Fig. 53).

The power cord should be connected to a grounded/earthed,

3-conductor power outlet. If the power outlet is not grounded/
earthed properly, Olympus can no longer warrant the electrical
safety performance of the equipment.

2. Plug the power cord plug 3 into the wall outlet | (Fig. 55).

Should the power cord come in contact with the hot lamp socket,

the power cord could melt and cause electric shock. Be sure
to route the power cord away from the lamp socket.

” (OFF) before

2

Fig. 54

Fig. 55

Operation Using the TL4 Power Supply Unit (Fig. 54)

1

} The following operation is not required when the LED lamp is used.

1. Insert the connector @ of the power cord firmly into the input connector
2 of the TL4 (Fig. 54).

2. Plug the power cord plug 3 into the wall outlet | (Fig. 55).

43

38

11

{ For safe use of the lamp socket, we recommend performing the following inspection periodically (every time you replace

the lamp bulb and at least every 6 months).
{ The table below identified the check items to be observed. Put (X) if not applicable or (
{ If there is any (

the lamp socket.
{ If you detect an abnormality other than that listed below or with other Olympus product, also stop the use of the product and

contact Olympus for detailed inspections.
{Note that the service, replacement and detailed inspections are charged after expiration of the warranty period.

1. More than 8 years have passed since original purchase or the total power
ON time exceeds 20,000 hours.

2. Lamp does not light sometimes even though the main switch is set to on.

3. Illumination flickers when you move the lamp cable or lamp socket.

4. Scorching or burning odor is produced during use.

5. Illumination still flickers after replacement with a new lamp bulb.

6. Deformation, backlash, or looseness, etc. when you assemble the lamp socket.

HALOGEN LAMP SOCKET INSPECTION SHEET

) if applicable.

) mark noted, immediately stop use of the product, and consult Olympus for detailed inspections or replace

If you have any questions, please contact Olympus.

Check results (Date)

Check items / / / /

7. Extreme discoloration of the lamp socket connection terminal or lamp socket
lamp bulb mount.

8. Discoloration, deformation or cracking of the lamp socket.

9. Melting, crack, deformation or solidification of the lamp cable or a wiring part.

10. Increased frequency of servicing compared to similar devices put into use at
the same time as the lamp socket.

* When the Check Result columns become insufficient, copy this sheet.

39

BX43

PROPER SELECTION OF THE POWER SUPPLY CORD

If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and
“ Certified Cord ” below:

CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the

electrical safety of the equipment.

Specifications

Voltage Rating
Current Rating
Temperature Rating
Length
Fittings Configuration

125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area)
6A minimum
60˚C minimum

3.05 m maximum
Grounding type attachment plug cap. Opposite terminates in molded-on IEC
configuration appliance coupling.

Table 1 Certified Cord

A power supply cord should be certified by one of the agencies listed in Table 1 , or comprised of cordage marked with an
agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of agencies listed in
Table 1. In case you are unable to buy locally in your country the power supply cord which is approved by one of the
agencies mentioned in Table 1, please use replacements approved by any other equivalent and authorized agencies in
your country.

Country Agency

Argentina

Australia SAA

IRAM Italy IMQ

Certification

Mark

Country Agency

Japan

JET, JQA, TÜV,
UL-APEX/MITI

Certification

Mark

Austria ÖVE Netherlands KEMA

Belgium CEBEC Norway NEMKO

Canada CSA Spain AEE

Denmark DEMKO Sweden SEMKO

Finland FEI Switzerland SEV

France UTE

Germany VDE U.S.A. UL

Ireland NSAI

United
Kingdom

ASTA
BSI

Table 2 HAR Flexible Cord

APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS

Printed or Embossed Harmoni-

Approval Organization

Comite Electrotechnique Belge
(CEBEC)

Verband Deutscher Elektrotechniker
(VDE) e.V. Prüstelle

Union Technique de I´Electricite´
(UTE)

Instituto Italiano del Marchio di
Qualita´ (IMQ)

British Approvals Service for Electric
Cables (BASEC)

N.V. KEMA KEMA-KEUR <HAR> 10 30 30

SEMKO AB Svenska Elektriska
Materielkontrollanstalter

Österreichischer Verband für
Elektrotechnik (ÖVE)

Danmarks Elektriske Materialkontroll
(DEMKO)

zation Marking (May be located
on jacket or insulation of internal
wiring)

CEBEC <HAR> 10 30 10

<VDE> <HAR> 30 10 10

USE <HAR> 30 10 30

IEMMEQU <HAR> 10 30 50

BASEC <HAR> 10 10 30

SEMKO <HAR> 10 10 50

<ÖVE> <HAR> 30 10 50

<DEMKO> <HAR> 30 10 30

Alternative Marking Utilizing
Black-Red-Yellow Thread (Length
of color section in mm)

Black Red Yellow

National Standards Authority of Ireland
(NSAI)

Norges Elektriske Materiellkontroll
(NEMKO)

Asociacion Electrotecnica Y
Electronica Espanola (AEE)

Hellenic Organization for
Standardization (ELOT)

Instituto Portages da Qualidade
(IPQ)

Schweizerischer Elektro
Technischer Verein (SEV)

Elektriska Inspektoratet SETI <HAR> 10 30 90

Underwriters Laboratories Inc. (UL) SV, SVT, SJ or SJT, 3 X 18AWG
Canadian Standards Association (CSA) SV, SVT, SJ or SJT, 3 X 18AWG

<NSAI> <HAR> 30 30 50

NEMKO <HAR> 10 10 70

<UNED> <HAR> 30 10 70

ELOT <HAR> 30 30 70

np <HAR> 10 10 90

SEV <HAR> 10 30 90

40

MEMO

MEMO

Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan

Wendenstrasse 14-18, 20097 Hamburg, Germany

3500 Corporate Parkway, Center Valley, Pennsylvania 18034-0610, U.S.A.

491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373

31 Gilby Road, Mount Waverley, VIC., 3149, Melbourne, Australia

5301

Blue Lagoon Drive, Suite 290 Miami, FL 33126, U.S.A.

Printed in Japan on April 00, 2010 M 010-01