Ocean Optics pH Sensor Patches, Probes and Cuvettes User Manual

pH Sensor Patches, Probes and Cuvettes

Installation and Operation Manual

Document Number pHSENSOR-TRANS-REFLECT-02-201111

Offices: Ocean Optics, Inc. World Headquarters

830 Douglas Ave., Dunedin, FL, USA 34698

Phone [phone]
Fax [phone]

8 a.m.– 8 p.m. (Mon-Thu), 8 a.m.– 6 p.m. (Fri) EST

E-mail: [e-mail] (General sales inquiries)

[e-mail] (Questions about orders)
[e-mail] (Technical support)

Additional
Offices:

Ocean Optics Asia

137 Xianxia Road, Suite 1802, Changning District, Shanghai, PRC. 200051

Phone [phone]

Fax [phone]
E-Mail [e-mail]

Ocean Optics EMEA

Geograaf 24, 6921 EW DUIVEN, The Netherlands

Phone [phone]

Fax [phone]
E-Mail

[e-mail]

Regional Headquarters

Maybachstrasse 11
73760 Ostfildern

Phone [phone]
Fax [phone]
E-Mail [e-mail]

Copyright © 2011 Ocean Optics, Inc.

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic,
mechanical, photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
This manual is sold as part of an order and subject to the condition that it shall not, by way of trade or otherwise, be lent, re-sold, hired out or
otherwise circulated without the prior consent of Ocean Optics, Inc. in any form of binding or cover other than that in which it is published.

Trademarks

All products and services herein are the trademarks, service marks, registered trademarks or registered service marks of their respective owners.

Limit of Liability

Ocean Optics has made every effort to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The
information provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to
any loss or damages arising from the information contained in this manual.

Table of Contents

About This Manual......................................................................................................... iii

Document Purpose and Intended Audience..............................................................................iii

Document Summary..................................................................................................................iii

Product-Related Documentation ............................................................................................... iii

Upgrades....................................................................................................................... iv

Chapter 1: Products...........................................................................1

Overview ....................................................................................................................... 1

Transmissive pH Patches/Probe.................................................................................... 1

System Components .................................................................................................................1

Hardware Setup and Installation ............................................................................................... 2

Probe Assembly Description ..................................................................................................... 3

Reflective pH Patches/Probe (nonintrusive)................................................................... 4

Sensor System Components.....................................................................................................4

Hardware Setup and Installation ............................................................................................... 5

Smart pH Cuvettes ........................................................................................................ 8

Sensor System Components.....................................................................................................8

Hardware Setup and Installation ............................................................................................... 9

Chapter 2: Desktop Software Installation and Operation ...............11

Calibrating the pH Sensor System................................................................................. 11

Using SpectraSuite........................................................................................................ 11

Performing a Factory Calibration with SpectraSuite .................................................................11

Performing a User Calibration with SpectraSuite...................................................................... 14

Taking pH Measurements with SpectraSuite ............................................................................ 16

Jaz Software Installation and Operation ........................................................................ 17

Performing a Factory Calibration with Jaz.................................................................................17

Performing a User Calibration with Jaz ..................................................................................... 20

Taking pH Measurements with Jaz ........................................................................................... 22

Appendix A: Algorithms Used ..........................................................25

PHSENSOR-TRANS-REFLECT-201111 i

Table of Contents

Appendix B: Troubleshooting...........................................................27

Overview ....................................................................................................................... 27

Transmissive pH Probes ........................................................................................................... 27

Reflective pH Patches ...............................................................................................................27

Smart pH Cuvettes .................................................................................................................... 28

All Form Factors ........................................................................................................................28

Sterilization.................................................................................................................... 28

Index ...................................................................................................29

ii PHSENSOR-TRANS-REFLECT-201111

About This Manual

Document Purpose and Intended Audience

This document provides you with installation and operation instructions for your pH Sensor components.
This manual includes information for the following Ocean Optics products:

 Transmissive pH Patch/Probe
 Reflective pH Patches/Probes (non-intrusive)
 Smart pH Cuvettes

Document Summary

Chapter Description

Chapter 1: Products Contains descriptions and specifications for Transmissive

and Reflective patches/probes and Smart Cuvettes.

Chapter 2: Desktop Software Installation and
Operation

Appendix A: Algorithms Used Lists the algorithms used for pH sensors.

Appendix B: Troubleshooting Contains troubleshooting suggestions.

Provides installation and operation instructions for
SpectraSuite and Jaz software.

Product-Related Documentation

 Jaz Installation and Operation Manual
 Smart pH Cuvettes Instructions
 Transmissive pH Probe Instructions
 Various USB spectrometer documents such as the USB4000 Spectrometer Installation and

Operation Manual.

 SpectraSuite Spectrometer Operating Software Installation and Operation Manual
 Various UV-Vis-NIR light source documents such as the Halogen Light Source (HL-

2000/HL-2000-LL/HL-2000-HP) Installation and Operation Manual

PHSENSOR-TRANS-REFLECT-201111 iii

About This Manual

You can access documentation for Ocean Optics products by visiting our website at

http://www.oceanoptics.com. Select Technical → Operating Instructions, then choose the appropriate

document from the available drop-down lists. Or, use the
of the web page.

Search by Model Number field at the bottom

You can also access operating instructions for Ocean Optics products on the

Resources

Engineering-level documentation is located on our website at

CD included with the system.

Technical → Engineering Docs.

Software and Technical

Upgrades

Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system.
To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise
Authorization (RMA) number. Please contact Ocean Optics for specific instructions when returning a
product.

iv PHSENSOR-TRANS-REFLECT-201111

Chapter 1

Products

Overview

Ocean Optics’ fully integrated pH systems provide full spectral analysis to help eliminate errors from dye
leaching or from changes in turbidity, temperature, and ionic strength. Inherent calibration based on the
physical properties of the immobilized indicator dye eliminates the need for frequent calibration. The
ratiometric algorithm provides accurate and reproducible measurements at a high resolution.

The pH sensor is designed to very fast response with high signal strength. These sensors are compatible
with aqueous solutions, ethanol/methanol solutions, ammonia, peroxides, and sodium hypochlorite
solutions.

The available sensor patches, probes and cuvette products include the following:

 Transmissive pH Patches/Probe
 Reflective pH Patches/Probe (nonintrusive)
 Smart pH Cuvettes

Transmissive pH Patches/Probe

System Components

The Transmissive pH Probe system consists of the following:

Transmissive pH Probe -- T-300/TP-300 sleeve combined with RE­BIF-BORO)

PHSENSOR-TRANS-REFLECT-201111 1

1: Products

Ocean Optics VIS/NIR desktop or Jaz spectrometer (slit size 25μm)

Light source (VIS/NIR: HL-2000, LS-1, LLS Series, etc.)

SpectraSuite software

Transmissive pH Patches, pack of 5 (PH-BCG-TRANS)

Calibration requires recording spectra in high and low pH samples, as
well as in at least one mid-range pH standard solution (such as a
NIST-traceable buffer).

For field measurements, an SD card with the pH software is required
for the Ocean Optics handheld Jaz spectrometer.

Hardware Setup and Installation

► Procedure

1. Connect the spectrometer to your computer using the supplied USB cable.

2. Install the light source as specified in its instructions. See Product-Related Documentation.

3. Attach the fibers between the spectrometer, probe, and light source. The transmissive probe
has two identical legs.

4. Turn on the light source and allow it to warm up for the period specified in the light source
instructions.

2 PHSENSOR-TRANS-REFLECT-201111

1: Products

5. The cap for the transmissive probe screws off. The probe uses a thin sensing film with
adhesive on one side; peel the patch into its two pieces, sometimes a razor-blade or long
finger-nails aide with this. Discard the non-transparent, non-sticky half.

transparent half that has one adhesive side (this is the sensor half).

affixed to the lens of the probe. Re-screw the cap back on the probe tip.

Keep the optically

This can be firmly

Probe Assembly Description

The picture below shows the individual components of the transmissive dip probe. Using a 0.05” Allen
key, the set screws at the base of the T-300 sleeve can be loosened and the sleeve can be removed from
the RE-BIF-BORO bundle. At the factory, the position of the fiber bundle in the sleeve is optimized for
best back reflection in liquid medium, and locked in that position.

Storage/Lifetime

Probes can be stored dry at room temperature for any amount of time. As a rule, once the maximum
absorbance at pH 11 falls below 0.1, the patch should be discarded and replaced

reference of pH 1). The patch’s lifetime depends on frequency of use, harshness of the samples it is
exposed to, the temperature of samples, and other environmental factors.

PHSENSOR-TRANS-REFLECT-201111 3

(assumes a

1: Products

Performance Specifications

Specification Ocean Optics pH Probe Value

pH range 5 – 9

Analytical Wavelength 620nm

Baseline Correction Wavelengths 512nm (isosbestic) or 750nm

Calibration Options User Calibration with 6 Buffers

Factory Calibration with 3 Buffers

Immediate Startup with No Buffers (requires undisturbed setup)

Typical Absorbance Range 0.20 – 0.40

Typical pK Range 6.1 – 6.5

Typical Slope Range 2.2 – 3.0

User Calibration Accuracy <1% of reading across range

Factory Reset Accuracy 0% at Reset Point

1% Within 1 pH unit of reset

Up to 4% at 3 pH units from reset

Resolution 0.02 pH

Response Time, t

Stability No detectable drift for up to 12 hours

Interferences Cannot be used with colored or turbid liquids

Baseline Correction Wavelengths 512nm (isosbestic) or 750nm

(90)

30 seconds

1% per day for long term monitoring

Yellow liquids can be used if the 750nm baseline is used

Reflective pH Patches/Probe (nonintrusive)

Sensor System Components

The nonintrusive Reflective pH Patch system consists of the following:

Reflective pH Probe – R1000-4-ANGLE

4 PHSENSOR-TRANS-REFLECT-201111

Ocean Optics VIS/NIR desktop or Jaz spectrometer (slit size 200μm)

Light source (VIS/NIR: HL-2000, LS-1, LLS series, etc.)

SpectraSuite software

Reflective pH Patches, pack of 5 (PH-BCG-REFLECT)

1: Products

Calibration requires recording spectra in high and low pH samples, as
well as in at least one mid-range pH standard solution (such as a
NIST-traceable buffer).

For field measurements, an SD card with the pH software is required
for the Ocean Optics handheld Jaz spectrometer.

Hardware Setup and Installation

Caution

Avoid touching the gold mesh material with bare fingers to prevent oils or disruptive
particles from interfering with sensor performance.

The reflective pH sensor patches are self-adhesive, and can be applied to the inner wall of an optically
clear container, either plastic or glass. The figure below shows the assembly of the patches.

PHSENSOR-TRANS-REFLECT-201111 5