Metrohm TitrIC pro II User Manual

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A

plicatio

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stallati

Th

TitrIC pro
lysis of w

titr

tion, and ion

determine

ductivity, p

centrations
trohm instru
tem at any
ameters.

alytical seq

A

1.

The sample
anion and c

2.

The sample
flow-throug
Afterwards
values) are

Th

whole pro

tware: The u
ntification int

is

one by Mag

rting titration

tia

oTM is done

data is concl

All
M

gIC Net.

Bulleti

on ins

II system is
ter samples

chromatogra

in a ve

, acid ca

f the individu

ents can b

time and

ence

is transferre

tion analysis

is transferre

cell to deter

emperature,

determined i

cedure is c

er enters th

MagIC Net

C Net. MagI

analysis, res

via RS232.

ded in a joint

354

ruction

used for th

using dire
phy. The foll
y short ti

acity and,

al anions an

incorporate

sed to det

to the two C

.

into an exter

ine the cond

H and acid c

the external

ntrolled by
sample po

nly. All sam

Net Comm

pectively tran

report contai

of Titr

fully autom

t measurem

wing parame
e: temperat
in parallel,

cations. Fur

in the exis

rmine additi

mpact ICs fo

al cell via th

uctivity.

pacity (p an

ell.

the MagIC
ition and sa
le liquid hand

nication suc

sfer of result

ing all result

C pro

atic

ent,

ers

re,
the
her

ting

nal

r

m

Net

ple

ling

as

to

by

I

Ta

ble of conte

1.

Delivery

-delivere

-Optional

Installati

2.

. 815 Rob

. Titrando

. Conduct

. 881 Com

. 881 Com

. Dosino h

3.

Cable co

Tubing c

4.

. External

. Installati

. Overvie

5.

Miscella

5.

. Membra

. Stirrer ....

. Electrod

6.

Software

6.

. Installati

ts:

ackage: .......

with TitrIC p

accessories:

n ..................

tic USB Sam

2.905.0010) .

meter (2.856

pact IC pro –

pact IC pro –

lder (6.2057.

nections ......

nnections .....

ell ................

n of the 50 m

of all the tub

eous .............

e Pumps of t

.....................

s ...................

.....................

n of the soft

.....................

ro II package

.....................

.....................

ple Processo

.....................

.0010) ...........

Anion – MCS

Cation (2.881

210) .............

.....................

.....................

.....................

L Dosing Uni

ing connectio

.....................

e 815 ..........

.....................

.....................

.....................

are ...............

.....................

....................

.....................

.....................

XL (1T/2P) ..

.....................

.....................

(2.881.0030)

.0010) ..........

.....................

.....................

.....................

.....................

....................

s .................

.....................

.....................

.....................

.....................

.....................

.....................

.... 3

.... 3

.... 3

.... 4

.... 4

.... 4

.... 4

... 5

.... 5

.... 5

.... 5

.... 5

.... 5

.... 6

.... 6

.... 7

.... 7

.... 7

.... 7

.... 8

.... 8

. Configur

.1. Devices .

.2. Solution .

.3. Swing H

.4. Lift positi

Fig

.: TitrIC pro II s

stem setup

.5. Needle ri

.6. Methods

. Configur

.1. Devices .

.2. Solution .

.3. Electrod

tion of MagI

.....................

.....................

ad configura

ns ................

nse position ..

.....................

tion of tiamo

.....................

.....................

s ...................

Net .............

.....................

.....................

ion ................

.....................

.....................

.....................

..................

.....................

.....................

.....................

.....................

.....................

.....................

.....................

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Page 1

.... 8

.... 8

.... 8

.... 8

.... 8

.... 8

.... 9

.... 9

.... 9

.... 9

.... 9

f 14

Application Bulletin 354

Installation Instruction of TitrIC pro II

6.3.4. Methods .................................................................... 9

6.4. Setup of RS232 communication between MagIC Net

and tiamo

TM

............................................................ 10

7. Complete Analysis with TitrIC ................................. 10

9 TitrIC pro – Combo Kit Dilution ............................... 11

9.1 Delivery package .................................................... 11

9.2 Installation .............................................................. 11

9.3 Dosino .................................................................... 11

9.4 Transfer Tubing ...................................................... 11

9.5 External Dilution Vessel .......................................... 11

9.6 Analysis .................................................................. 11

9 TitrIC pro – Combo Kit Partial Loop ........................ 13

9.1 Delivery package .................................................... 13

9.2 Installation .............................................................. 13

9.3 Rinsing station ........................................................ 13

9.4 MiPT Dosino ........................................................... 13

9.5 Analysis .................................................................. 13

Page 2 of 14

Application Bulletin 354

Installation Instruction of TitrIC pro II

1. Delivery package:

-delivered with TitrIC pro II package:

No. Article no. Article designation

IC

1 2.881.0010 881 Compact IC pro - Cation

1 2.881.0030 881 Compact IC pro - Anion - MCS

2 2.850.9010 IC Conductivity Detector

Sample processor

1 2.815.0020 815 Robotic USB Sample Processor

XL (1T/2P)

1 2.786.0040 Swing Head

1 6.1462.040 Robotic Arm with transfer head for

786 Swing Head, right swinging

1 6.2833.020 Needle holder 1/8 in. With tubing

connection M6

1 6.1835.050 PEEK sample aspiration tube (1.58

mm ID)

1 6.2629.000 Screw nut to needle holder IC

Conductometry

2.856.0010 856 Conductivity Module

1 6.0915.100 Five-ring conductivity measuring cell,

c = 0.7 cm-1, with Pt 1000

1 6.2151.000 Cable USB A - mini-DIN 8P (R)

1 6.2763.000 Flow through cell for 60915100

p and m value titration (pH measurment)

1 2.905.0010 905 Titrando

1 6.0277.300 iAquatrode plus with Pt 1000

1 6.2151.000 Cable USB A – mini-DIN 8P

1 6.2307.230 Buffer solutions pH 4, 7 and 9

1 6.2325.000 Phit kit

Liquid Handling

2 2.800.0010 800 Dosino

1 6.3032.220 807 Dosing Unit, glass, 20 mL

1 6.3032.250 807 Dosing Unit, glass, 50 mL

1 6.2057.210 Holder Dosino for IC Instruments

1 6.1608.030 Glass bottle GL45 1L (round)

1 6.1808.280 Adapter Dosino Port 4 / M6

1 6.1618.020 Thread adapter S 40 to GL45

2 6.2744.080 Coupling M6-UNF 10/32 (Dosing Unit

– IC screw)

1 6.2744.070 Pressure screw made of PEEK,

short, 5x

1 6.1808.010 Coupling piece 2 x M6 outer threads

1 6.1808.060 T Connector M6

Connection tubings

1 6.1805.060 Connection tubing FEP, L = 60 cm, 2

x M6 thread

2 6.1805.110 Connection tubing FEP, L = 80 cm, 2

x M6 thread

1 6.1805.030 Connection tubing FEP, L = 150 cm,

2 x M6 thread

External cell

1 2.802.0020 802 Rod Stirrer

1 6.2001.070 Stand support for 804

1 6.1414.060 Titration vessel lid micro ECO

1 6.1415.250 Titration vessel / 50-150 mL

5 6.2730.030 Stopper with nipple and O-ring

1 6.2730.080 Screw nipple M16/NS14

1 6.2730.020 Septum stopper

3 6.2730.060 Screw nipple for electrodes

1 6.1543.170 Aspiration tip M8

1 6.2709.090 Stopper (12 mm)

1 6.1543.210 3-way stopper with antidiffusion valve

Software

1 6.6056.231 Tiamo 2.3 light CD: 1 Licence

1 6.6059.232 MagIC Net 2.3 Professional CD: 1

Licence

RS232 Communcation MagIC - Tiamo

1 6.2134.040 Connecting cable RS-232 to IMP-PC

(DBp)

2 6.2148.050 USB/RS-232 Converter for 900

Touch Control / Ti-Touch

-Optional accessories:

1 6.2041.xxx Sample rack made of PVC

1 6.1432.xxx Sample beaker

1 6.2324.000 Conductivity standard (100 µS/cm)

1 6.2301.060 Conductivity standard (12.88 mS)

1 6.2307.230 Buffer solutions 3 x 10 x 30mL of pH

4 / 7 / 9

1 6.2065.000 Stacking frame for 846 Dosing

Interface, 856 Conductivity
Module,867 pH Module

1 6.2041.800 Sample rack 100* 75 ml

1 6.2041.810 Sample rack 34* 150 ml

1 6.2041.820 Sample rack 28* 250 ml

1 6.2041.840 Sample rack 59* 120 ml

Page 3 of 14

Application Bulletin 354

Installation Instruction of TitrIC pro II

1 6.1432.210 Sample beaker 75 mL made of glass

(for 6.2041.800)

1 6.1432.320 Sample beaker 250 mL made of

glass (for 6.2041.820)

1 6.1453.250 Sample beaker 250 mL made of

polypropylene (for 6.2041.820)

1 6.1459.300 Sample beaker 120 mL made of

polypropylene (250 pieces) (for

6.2041.840)

1 6.1006.510

Metrosep A Supp 5 - 100/4.0

1 6.1006.520 Metrosep A Supp 5 - 150/4.0

1 6.1006.500 Metrosep A Supp 4/5 Guard/4.0

1

6.1030.410 Metrosep A Supp 15 – 100/4.0

6.1030.420 Metrosep A Supp 15 – 150/4.0

1 6.1030.500 Metrosep A Supp 15 Guard/4.0

1 6.1050.410 Metrosep C 4 – 100/4.0

1 6.1050.420 Metrosep C 4 – 150/4.0

1 6.1050.500 Metrosep C 4 Guard/4.0

1 2.849.1030 849 Level Control for Inline Eluent

Preparation

2. Installation

The following is a detailed description of the TitrIC pro II
installation.

We strongly recommend that the individual steps are carried
out in the order given below!

2.1. 815 Robotic USB Sample Processor XL (1T/2P)

The sample processor is placed at the side at which it is
most easily accessible. Please note that the following
procedure refers exclusively to the version in which the
sample processor is located at the left-hand side of the IC
system.

 On the left-hand side of the 815 Robotic USB Sample

Processor XL, attach the stand support (6.2001.070)
for the external titration vessel.

 Mount the Swing Head (2.786.0040) onto the 815 (see

installation instructions).

 Do not screw the robotic arm (6.1462.040) onto the

Swing Head!

 The Sample Rack supplied is screwed onto the 815.

 In the vicinity of the 815 the two canisters (standard

equipment of 815) are placed on the floor: One 10 L
canister is used as a waste container, the second one
as a reservoir for deionized (DI) water used for rinsing
the external cell.

 From the smaller opening of the canisters containing DI

water, an M8 PTFE tubing connection of suitable length
is led to the connection box made of white plastic of
membrane pump 1 / tower 1 and connected.

 From the front opening of the waste canister an M8

PTFE tubing connection of suitable length is led directly
to membrane pump 2 / tower 1 (black plastic) and
connected.

A more detailed description is given in the instructions for
use of the 815 Robotic USB Sample Processor XL.

2.2. Titrando (2.905.0010)

Directly to the right of the 815 Robotic USB Sample
Processor, place the Titrando with a bottle of titration
solution (0.1 mol/L HCl will be connected to the 20 mL
Dosing Unit and 800 Dosino).

2.3. Conductometer (2.856.0010)

The 856 Conductometer is placed directly at the left of the
Titrando. For detailed information about the usage check the
Instructions of Use of the 856.

Before measurement it is recommended to carry out the
calibration of the cell constant in the flow-through cell of this
setup. For further information consult the Installation
Instructions of the 856 Conductometer.

Page 4 of 14

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in 354

ction of TitrIC

ro II

2.4. 881 C

Pl

ce the 881 C

to

the right of t

installed. Pl

be

mpact IC pro

Co

2.5. 881 C

ce the 881

Pl
rig

t of the 881

ins

trument can

tructions for

In

2.6. Dosin

Dosino hol

sid

e of the 881

032.250) D

(6.
lat

r stage.

3.

Cable con

Fi

st all the

truments. B

itched off.

Controll

Conduct
Process

2x USB

Compac

Controll

Controll

Connect

(transfer
injection
Anion –

Connect

(0.1 mol

Controll

USB 2 o

4.

Tubing co

4.1. Exter

numbers gi

Th

drawing bel

In

he next step

co

ponents a

(6.

1414.060):

small 6.27

Th
ar

screwed loo

in

osition when

(2.881

mpact IC p

.0030)

ompact IC pr

e Titrando.
ase consult t
instruments.

mpact IC p

ompact IC p
Compact IC

already be
se for the C

o holder (6.2

der (6.2057.

ompact IC

sing Unit is

ections

power cabl

oth the inst

r cable USB

ometer to US

r.

able (6.2151

IC pro to P

r cable USB

r, do not co

the MSB cab

to external ti

valves) to th

MCS at MSB

the MSB cab

L HCl) to 90

r cable USB

the 815 Sa

nections

al cell

en here ( ,

w.

the external

e inserted

0.030 stopp
ely into ope

the buret tips

o – Anion –

– Anion –

he IC instru

e Instruction

o – Cation (2

o –Cation (2

pro – Anion

installed. Pl

mpact IC pro

57.210)

10) is place
ro – Anion –

crewed ont

s are co

uments and

6.2151.000)

1 of the 81

.020) from th
, do not con

6.2151.000)
nect!

le from Dosin
ration cell an

881 Compa

1

le from Dosin
MSB 1

6.2151.000)
ple Process

...) and “ope

cell is prepar

in the titr

rs (with the

ings 1, 2, 3,

, etc. are ins

CS

CS (2.881.0

ent can alre

s for Use for

.881.0010)

881.0010) to
– MCS. Th

ase consult
instruments.

on the left

MCS; the 50

this holder

nected to

the PC re

rom 856
Sample

two 881
ect!

rom PC to 81

800, 50 mL

to the IC

t IC pro –

800, 20 mL

rom 905 to

r

ing 1”, ... ref

d. The follo

tion vessel

lack rubber r

and 7 and fi

rted.

30)

ady

the

the
IC
the

top
mL

t a

the

ain

5

r to

ing

lid

ing)
xed

A

eptum stop

e 6.2730.08

Th

ning 9 and

op

nings 4, 8 a

Th

e titration v

2001.070) u

(6.

ght with the

he

nd support i

st

ew.

Fi

.: Numbers an

(6.

414.060)

The rin

This op

The rin

This is

The rin

The 3-

is insert
the dist
pump 1
connect

is insert
the dist
pump 1
connect

is insert
the dist
pump 1
connect

815).

(6.1543
20 mL
with the
cm, sup
Further
connect
(6.1805

er (6.2730.0
screw nippl

the 6.2730

d 6.

ssel lid is a
ing opening

lamping ring
mounted on

connections o

ing nozzle (6.

d into openi
ibutor (6.180
/ tower 1 of t

on (6.1805.0

ning remains

ing nozzle (6.

d into openi
ibutor (6.180
/ tower 1 of t

on (6.1805.0

here the pH

ing nozzle (6.

d into openi
ibutor (6.180
/ tower 1 of t

on (6.1805.0

ay stopper wi

210) is put in

osing Unit (0
FEP-tubing c

lied with the

he port 1 of t

d with an FE
110, 80 cm) t

0) is put int
e M16/NS14
.060 screw

ttached to t

10. It is fixe
and the horiz

the 815 with

n the titration v

2740.020, su

g 1, which is
.170) to the

e 815 with th

0, supplied

empty.

2740.020, su

g 3, which is
.170) to the

e 815 with th

0, supplied

lectrode is i

2740.020, su

g 5, which is
.170) to the

e 815 with th

0, 60 cm, su

th antidiffusio

o opening 6.
1 mol/L HCl)

onnection (6.

807) to the 3
e 50 mL Do

P-tubing con

o the 3-way s

the openin
is screwed

nipple M16

e stand sup

at the requ

ntal brace of
the socket h

ssel lid

plied with 81

connected vi

embrane
FEP-tubing

ith 815).

plied with 81

connected vi

embrane
FEP-tubing

ith 815).

serted.

plied with 81

connected vi

embrane
FEP-tubing
plied with

valve
Port 1 of the
is connected

805.100, 40

way stopper.

ing Unit is

ection
opper.

6.
into
into

port

ired

the

ead

5)

5)

5)

Page 5

f 14