Metrohm TitrIC pro I User Manual

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1.

Scope of

- deliver

- Option

2.

Installati

5.

815 Rob

Titrando

Conduct

Bottle ho

5.

881 Com

Dosino h

3

Cable co

Tubing c

5.

External

Installati

Overvie

5

Miscella

5.

Membra

5.

Stirrer ....

Length o

ts

delivery: .......

d with TitrIC

l accessories

n ..................

tic USB Sam

2.905.0010) .

meter (2.856

der for Dosin

pact IC pro –

lder (6.2057.

nections ......

nnections .....

ell ................

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of all the tub

eous .............

e Pumps of t

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the aspiratio

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: ....................

.....................

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.0010) ...........

s (6.2061.01

Anion – MCS

210) .............

.....................

L Dosing Uni

ing connectio

.....................

e 815 ..........

.....................

n tips in the e

.....................

....................

.....................

XL (1T/2P) ..

.....................

0) .................

(2.881.0030)

.....................

....................

s .................

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ternal cell ....

.... 2

.... 3

.... 4

... 4

.... 4

.... 5

.... 6

.... 7

Fig

.: TitrIC pro I S

stem

Electrod

6

Software

6.

Installati

6.

Configur

.1 Devices .

.2 Solution .

.3 Swing H

.4 Lift positi

.5 Needle ri

.6 Methods

Configur

Devices .

Solution .

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Page 1

.... 7

.... 8

.... 9

f 14

Application Bulletin 353

Installation Instructions of TitrIC pro I

6.7 Methods .................................................................... 9

6.8 Setup of RS232 communication between MagIC Net

and tiamo

TM

.............................................................. 9

7 Complete Analysis with TitrIC ................................. 10

8 TitrIC pro – Combo Kit Dilution ............................... 11

8.1 Delivery package .................................................... 11

8.2 Installation .............................................................. 11

8.3 Dosino .................................................................... 11

8.4 Transfer Tubing ...................................................... 11

8.5 External Dilution Vessel .......................................... 11

8.6 Analysis .................................................................. 11

9 TitrIC pro – Combo Kit Partial Loop ........................ 13

9.1 Delivery package .................................................... 13

9.2 Installation .............................................................. 13

9.3 Rinsing station ........................................................ 13

9.4 MiPT Dosino ........................................................... 13

9.5 Analysis .................................................................. 13

1.

1. Scope of delivery:

- delivered with TitrIC pro I package:

No. Article no. Article designation

IC

1 2.881.0030 881 Compact IC pro - Anion

1 2.850.9010 IC Conductivity Detector

Sample processor

1 2.815.0020 815 Robotic USB Sample

Processor XL (1T/2P)

1 2.786.0040 Swing Head

1 6.1462.040 Robotic Arm with transfer head for

786 Swing Head, right swinging

1 6.2833.020 Needle holder 1/8 in. With tubing

connection M6

1 6.1835.050 PEEK sample aspiration tube (1.58

mm ID)

1 6.2629.000 Screw nut to needle holder IC

Conductometry

1 2.856.0010 856 Conductivity Module

1 6.0915.100 Five-ring conductivity measuring

cell, c = 0.7 cm-1, with Pt 1000

1 6.2151.000 Cable USB A - mini-DIN 8P (R)

1 6.2763.000 Flow through cell for 60915100

p and m value titration (pH measurment)

1 2.905.0010 905 Titrando

1 6.0277.300 iAquatrode plus with Pt 1000

1 6.2151.000 Cable USB A – mini-DIN 8P

1 6.2307.230 Buffer solutions pH 4, 7 and 9

1 6.2325.000 Phit kit

Ca and Mg titration

1 6.0510.100 Combined polymermembrane

electrode Ca

1 6.2104.020 Electrode cable / 1m / F

1 6.2327.000 Electrolyte solution c(NH4NO3) =1

mol/L

Liquid Handling

4 2.800.0010 800 Dosino

1 6.3032.210 807 Dosing Unit, glass 10 mL

2 6.3032.220 807 Dosing Unit, glass, 20 mL

1 6.3032.250 807 Dosing Unit, glass, 50 mL

1 6.2057.210 Holder Dosino for IC Instruments

1 6.2061.010 Bottle holder for Dosinos

3 6.1608.030 Glass bottle GL45 1L (round)

1 6.1808.280 Adapter Dosino Port 4 / M6

Page 2 of 14

Application Bulletin 353

Installation Instructions of TitrIC pro I

1 6.1618.020 Thread adapter S 40 to GL45

2 6.2744.080 Coupling M6-UNF 10/32 (Dosing

Unit – IC screw)

1 6.2744.070 Pressure screw made of PEEK,

short, 5x

1 6.1808.010 Coupling piece 2 x M6 outer threads

1 6.1808.060 T Connector M6

Connection tubings

3 6.1805.060 Connection tubing FEP, L = 60 cm,

2 x M6 thread

2 6.1805.110 Connection tubing FEP, L = 80 cm,

2 x M6 thread

1 6.1805.030 Connection tubing FEP, L = 150 cm,

2 x M6 thread

External cell

1 2.802.0020 802 Rod Stirrer

1 6.2001.070 Stand support for 804

1 6.1414.060 Titration vessel lid micro ECO

1 6.1415.250 Titration vessel / 50-150 mL

5 6.2730.030 Stopper with nipple and O-ring

1 6.2730.080 Screw nipple M16/NS14

1 6.2730.020 Septum stopper

3 6.2730.060 Screw nipple for electrodes

1 6.1543.170 Aspiration tip M8

1 6.2709.090 Stopper (12 mm)

1 6.1543.210 3-way stopper with antidiffusion

valve

Software

1 6.6056.231 Tiamo 2.3 light CD: 1 Licence

1 6.6059.232 MagIC Net 2.3 Professional CD: 1

Licence

RS232 Communcation MagIC - Tiamo

1 6.2134.040 Connecting cable RS-232 to IMP-

PC (DBp)

2 6.2148.050 USB/RS-232 Converter for 900

Touch Control / Ti-Touch

1 6.2301.060 Conductivity standard (12.88 mS)

1 6.2307.100

Buffer solutions 500 mL, pH 4 / 7 / 9

/110/120

1 6.2307.230 Buffer solutions 3 x 10 x 30mL of pH

4 / 7 / 9

1 6.2065.000 Stacking frame for 846 Dosing

Interface, 856 Conductivity Module,867 pH Module

1 6.2041.800 Sample rack 100* 75 ml

1 6.2041.810 Sample rack 34* 150 ml

1 6.2041.820 Sample rack 28* 250 ml

1 6.2041.840 Sample rack 59* 120 ml

1 6.1432.210 Sample beaker 75 mL made of

glass (for 6.2041.800)

1 6.1432.320 Sample beaker 250 mL made of

glass (for 6.2041.820)

1 6.1453.250 Sample beaker 250 mL made of

polypropylene (for 6.2041.820)

1 6.1459.300 Sample beaker 120 mL made of

polypropylene (250 pieces) (for

6.2041.840)

- Optional accessories:

1 6.2041.xxx Sample rack made of PVC

1 6.1432.xxx Sample beaker

1 6.1030.xxx Anion column Metrosep A Supp 15

1 6.1030.xxx Guard column Metrosep A Supp 15

1 6.1050.xxx Cation column Metrosep C 4

1 6.1050.xxx Guard column Metrosep C 4

1 6.2324.000 Conductivity standard (100 µS/cm)

Page 3 of 14

Application Bulletin 353

Installation Instructions of TitrIC pro I

2. Installation

The following is a detailed description of the TitrIC pro I installation.

We strongly recommend that the individual steps are carried out in the order given below!

5.1 815 Robotic USB Sample Processor XL (1T/2P)

The sample processor is placed at the side at which it is most easily accessible. Please note that the following procedure refers exclusively to the version in which the sample processor is located at the left-hand side of the IC system.

 On the left-hand side of the 815 Robotic USB Sample

Processor XL, attach the stand support (6.2001.070) for the external titration vessel.

 Mount the Swing Head (2.786.0040) onto the 815 (see

installation instructions).

 Do not screw the robotic arm (6.1462.040) onto the

Swing Head!

 The Sample Rack supplied is screwed onto the 815.

 In the vicinity of the 815 the two canisters (standard

equipment of 815) are placed on the floor: One 10 L canister is used as a waste container, the second one as a reservoir for deionized (DI) water used for rinsing the external cell.

 From the smaller opening of the canisters containing DI

water, an M8 PTFE tubing connection of suitable length is led to the connection box made of white plastic of membrane pump 1 / tower 1 and connected. For further information consult the Installation Instructions of the 815 Robotic USB Sample Processor XL.

 From the front opening of the waste canister an M8

PTFE tubing connection of suitable length is led directly to membrane pump 2 / tower 1 (black plastic) and connected. For further information consult the Installation Instructions of the 815 Robotic USB Sample Processor XL.

A more detailed description is given in the instructions for use of the 815 Robotic USB Sample Processor XL.

5.2 Titrando (2.905.0010)

Directly to the right of the 815 Robotic USB Sample Processor, place the Titrando with a bottle of titration solution (0.1 mol/L HCl, will be connected to the 20 mL Dosing Unit and 800 Dosino).

5.3 Conductometer (2.856.0010)

The 856 Conductometer is placed directly at the left of the Titrando. For detailed information about the usage check the Instructions of Use of the 856.

Before measurement it is recommended to carry out the calibration of the cell constant in the flow through cell of this setup. For further information consult the Installation Instructions of the 856 Conductometer.

5.4 Bottle holder for Dosinos (6.2061.010)

The bottle holder for Dosinos is placed onto the 856 Conductometer. This bottle holder is used for the two solutions for the Calcium and Magnesium Titration. Solution 1 is 0.2 mol/L TRIS with 0.1 mol/L Acetylacetone. Solution 2 is 0.05 mol/L Na

EDTA

2

5.5 881 Compact IC pro – Anion – MCS (2.881.0030)

Place the 881 Compact IC pro – Anion – MCS (2.881.0030) to the right of the Titrando. The IC instrument can already be installed. Please consult the Instructions for Use for the Compact IC pro instruments.

5.6 Dosino holder (6.2057.210)

The Dosino holder (6.2057.210) is placed on the left top side of the 881 Compact IC pro – Anion – MCS; the 50 mL (6.3032.250) Dosing Unit is screwed onto this holder at a later stage.

3 Cable connections

First all the power cables are connected to the instruments. Both the instruments and the PC remain switched off.

Controller cable USB (6.2151.000) from 856 Conductometer to USB 1 of the 815 Sample Processor.

USB cable (6.2151.020) from the 881 Compact IC pro to PC, do not connect!

Controller cable USB (6.2151.000) from PC to 815 Controller, do not connect!

Connect the MSB cable from Dosino 800, 50 mL (transfer to external titration cell and to the IC injection valves) to Anion 881 MSB 1.

Connect the MSB cable from Dosino 800, 20 mL (0.1 mol/L HCl) to 905 MSB 1.

Controller cable USB (6.2151.000) from 905 to USB 2 of the 815 Sample Processor.

Connect the MSB cable from Dosino 800, 10 mL (0.05 mol/L Na2EDTA) to 905 MSB 2.

Connect the MSB cable from Dosino 800, 20 mL (0.2 mol/L TRIS, 0.1mol/L acetylacetone) to 905 MSB 3.

Page 4 of 14

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The PT inserted distribut tower 1 connecti

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After th screwed (2.802.0 the tow USB Sa

in 353

ctions of TitrIC

ng nozzle (6.

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Page 5

f 14

Application Bulletin 353

Installation Instructions of TitrIC pro I

 Port 1 is used for transferring the sample to the

external titration vessel using FEP-tubing 6.1805.110 (80 cm, supplied by the 815 Sample Processor)

 Port 2 is used for aspirating the sample via conductivity

flow through cell which is connected to the titration arm. The FEP-tubing 6.1805.110 is used here (80 cm, again supplied by the 815 Sample Processor).

 Port 3 is used by for sample transfer to the IC system.

The transition from M6 to the IC screw size (1/16 in.) is achieved with the 6.2744.080 adapter. A PTFE-capillary (0.75 mm ID) is used to aspirate the sample from the T piece at the swing arm through the anion injection valve into the 50 mL Dosino. At the T piece the adapter M6 thread to UNF 10/32 (6.2744.080) is used.

 Port 4 requires a coupling between Luer and M6

(6.1808.280): This port is used for emptying the Dosing Unit into the waste canister. Herefore the FEP-tubing (6.1805.030) with 150 cm length is used.

Once the tubing connections have been made, the Dosing Unit is attached to the Dosino holder (6.2057.210) with the thread adapter 6.1618.020.

4.3 Overview of all the tubing connections

The three 60 cm FEP-tubing connections (6.1850.060, supplied with the 815) are used to connect the membrane pump 1 / tower 1 of the 815 and the three rinsing nozzles (6.2740.020, supplied with 815) to the openings 1,3, and 5 of the external cell (see chapter 4.1).

The 60 cm M8-PTFE-tubing connection (6.1805.510) is used to connect the membrane pump 2 / tower 1 of the 815 and the M8-PTFEaspiration tip (6.1543.170) to the opening 7 of the external cell (see chapter 4.1).

The Robotic Arm (6.1462.040) is equipped with a PEEK aspiration tube (6.1835.050). The aspiration tube can be mounted using the screw nut (6.2629.000) and the needle holder (1/8 in with tubing connection M6, 6.2833.020). The coupling piece with two outer M6 connections (6.1808.010) is used to connect the needle to the T Connector M6 (6.1808.060).

Two 80-cm-FEP-tubing connections (6.1850.110) are used to connect the T piece (6.1808.060) at the robotic arm with the flow through cell (6.2763.000) for the conductivity measurement and the flow through cell with the 50 mL Dosino at Port

2. The third 80-cm-FEP-tubing (6.1850.110, supplied with the 815) is used to connect the 50 mL Dosino Port 1 to the external titration vessel at

the left of the 815.

The 40-cm-FEP-tubing connection (6.1805.100, supplied with the Dosing Unit) is used to connect port 1 of the 20 mL Dosing Unit (0.1 mol/L HCl) with the 3-way stopper in the external cell (opening 6 in chapter 4.1)

The 150-cm-FEP-tubing connection (6.1805.030) is used to connect port 4 of the 50 mL Dosing Unit to the 10 L PE waste canister (6.1621.000, supplied with the 815).

The 60-cm-FEP-tubing connection (6.1805.060) is used to connect port 1 of the 10 mL Dosing Unit (0.05 mol/L Na2EDTA) the external cell (opening 6 in chapter 4.1).

The 60-cm-FEP-tubing connection (6.1805.060) is used to connect port 1 of the 20 mL Dosing Unit (0.2 mol/L TRIS / 0.1 mol/L acetylacetone) with the external cell (opening 2 in chapter 4.1).

Suitable lengths of the 0.97 mm PTFE capillary (6.1803.020, supplied with the 881) are used to connect port 3 of the 50 mL Dosing Unit (using the screws 6.2744.080 and 6.2744.070) and the injection valve of the 881 Compact IC pro instrument that is again connected to the T-piece (6.1808.060) at the robotic arm (using the screws

6.2744.080 and 6.2744.070). In this setup and methods the following sequential arrangement was implemented: T-piece – anion injection valve – 50 mL Dosino.

5 Miscellaneous

5.1 Membrane Pumps of the 815

The membrane pump 1 at tower 1 is used to supply the DI water for rinsing of the external vessel; the membrane pump 2 is used for emptying the external vessel.

Connect the membrane pump 2/tower 1 to the left connector of the Distributor leading to the M8 aspiration tip for emptying the external vessel. The upper part of the membrane pump 2 is connected to the waste canister.

The upper part of membrane pump 1 is connected to the right connector of the Distributor leading to the three M6 rinsing nozzles to rinse the external vessel. The lower part of the membrane pump 1 is connected to the DI-water canister.

5.2 Stirrer

The 802 Rod Stirrer is inserted through opening 9 of the external cell and connected to the tower 1 of the 815. The propeller (6.1909.050) is pushed on from below.

Page 6 of 14

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1.

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

Conductivit cable is con the 856 Co into the flo through cell support (6.

6

Software

6.1

Installation



tiamo

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

MagIC Net

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

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he aspiratio

tration vessel

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h the middle

trode (iAquat is inserted in

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cell (6.0915.

nected to the

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of the softw

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2.

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ith the 854 i

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6.2763.000).

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time then the

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suring cell

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in 353

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Fi

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N

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osing Port 1

osing Port 2

ill Port

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Sa

ple Process

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‘

C

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Port 3

Port 2

Port 4

table of the 50

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und

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160.0

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Page 7

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6.

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Th

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

TitrIC pro I:

Depending carried out:

Value 1 = 1 ion chromat Value 1 = 2 a calibratio Value 1 = 3



This metho method in t



TitrIC pro I_

for calibrati used in co

I_ conductiv

‘I

tower 1

Manu

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k is used f position is nu o the last p ter is placed

s

ethods (.imet

his method i n the value

files) are pr

the standar

f

Value 1

complete a

alysis includi

ography.

only an ion

hromatograp

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is used in c

n analysis

mbination wit

amoTM.

onductivity c

n of the cond

bination with

ity calibration

libration:

uctivity sens the tiamoTM

.

815

Rack p

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seconds.

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ample Chan

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ethod

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pplication Bulle

stallation Instr



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used to det used in tia

tiamoTM m



TitrIC pro I_

used to det solution us with the tia

of EDTA

Im

port: Click o

M

thod manage

rel

ted to TitrIC

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N

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Alt

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

Shut down M

6.

Configurat

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Devices

6.

C

nnected US

tia

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6.

Solution

Th

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10

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M m

l/L TRIS / 0.

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.

in 353

ctions of TitrIC

iter determina

rmine the tit o. It has to b thod

iter determina

rmine the tit d in tiamo. It

oTM method

.

Method

… / Edit /

pro I from th

e folder exa

se methods

ministrator le

IC Net.

gIC Net

on of tiamo

started.

devices are

ed. After co

est the d

ep the propo

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MSB 1 port

nit (0.05 mo

5. The Dosin

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5. In order t

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ick on ‘

Titra

ts/Solutions’

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Solu

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at t

Config

an

pro I

tion of HCl:

T

r of the 0.1

used in com

I_titer deter

tion of EDTA:

r of the 0.05

has to be use

TitrIC pro I_tit

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mport

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MagIC Net

ple/TitrIC of

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be defined i

Dosing Unit of the 905.

l/L Na2EDTA

of the 20 m

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ensure co

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Concentrati

is method is ol/L HCl solu bination with

ination of HC

This method i

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d in combinat

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he Dosino of

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Dosing Unit

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the the

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t

Fi

.: Configuratio

N

w the tubin sing unit und

table for titrant

lengths an

r ‘

Edit/Proper

s/solutions

ports are

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efined for

unit’

as follo

ach

s:

Page 8

f 14

Application Bulletin 353

Installation Instructions of TitrIC pro I

HCl

Port Length Diameter

Dosing Port

Dosing Port 1 - -

Prep/Empty

Dosing Port 1 Port 1 40 cm 2.0 mm

Dosing Port 2 Port 3 0 cm 0.0 mm

Fill Port Port 2 25 cm 2.0 mm

Special Port Port 4 0 cm 0.0 mm

EDTA

Port Length Diameter

Dosing Port

Dosing Port 1 - -

Prep/Empty

Dosing Port 1 Port 1 60 cm 2.0 mm

Dosing Port 2 Port 3 0 cm 0.0 mm

Fill Port Port 2 25 cm 2.0 mm

Special Port Port 4 0 cm 0.0 mm

TRIS/acetyacetone

Port Length Diameter

Dosing Port

Dosing Port 1 - -

Prep/Empty

Dosing Port 1 Port 1 60 cm 2.0 mm

Dosing Port 2 Port 3 0 cm 0.0 mm

Fill Port Port 2 25 cm 2.0 mm

Special Port Port 4 0 cm 0.0 mm

Fig.: Configuration table of the Dosing Units

6.6 Electrodes

 pH electrode: Under ‘Configuration/Sensors’ click on

‘iAquatrode Plus’, <Ok>.

check the information and click on

 Ca ISE electrode: Under ‘Configuration/Sensors’ click

on ‘Edit/New/ISE electrode and enter under ‘Sensor

name’ Ca-ISE

and click on

 Conductivity sensor: Under ‘

Edit/New/other sensor’ and enter under ‘Sensor

on ‘

name’ Conductivity sensor

, type in the desired sensor information

<Ok>.

Configuration/Sensors’ click

type in the desired sensor

information and click on <Ok>.

6.7 Methods

The following methods (.mmet files) are provided for

TM

tiamo

:

 TitrIC pro I: This method is the standard method used. It

does the complete titration analysis and sends the

results to MagIC Net. It is used in combination with

TitrIC pro I method in MagIC Net.

In tiamo it is typically used as a single determination in the autorepeat mode. In other words, it is always ready to do a titration as soon MagIC requires it. To set it into autorepeat go to the Run window, right-mouse-click and

Properties Run window’. Under Process select

select ‘

Autorepeat determination’. Close the window with <Ok>.

‘



TitrIC pro I_pH calibration manual: Here the iAquatrode

is taken out of the titration vessel and placed into the

TM

buffer solutions. Start this method only from tiamo



TitrIC pro I_titer determination of HCl: This method is

!

used for the titer determination. It has to be used in combination with the MagIC Net method

determination of HCl

TitrIC pro I_titer determination of EDTA: This method is



.

TitrIC pro I_titer

used for the titer determination. It has to be used in combination with the MagIC Net method

determination of EDTA

.

TitrIC pro I_titer

 TitrIC pro I_conductivity calibration: This method is used

for calibration of the conductivity sensor. It has to be used in combination with the MagIC Net method

pro I_calibration conductivity

.

TitrIC

Import: Click on

Method manager… / Edit / Import…

Method at the left, then select under File /

Import the methods related to TitrIC pro I also from the MagIC Net CD. You will find the methods in the folder example/TitrIC of the CD.

Note!

Alterations of these methods should only be carried out by a person at the administrator level and who is thoroughly familiar with tiamo

TM

.

 Shut down tiamo

6.8 Setup of RS232 communication between MagIC Net

and tiamo

The communication, e.g. result transfer between MagIC Net and tiamoTM is done via RS232. MagIC Net is the master in this setup. To establish the communication follow the steps below:

Make sure MagIC Net and tiamo the PC has two built-in RS232 ports you can connect the RS232 cable (6.2134.040) directly between the two ports. If the PC does not have the built-in RS232 ports please connect two USB/RS232 Converters (6.2148.050) to two USB ports. You can e.g. use the USB hubs of the IC

TM

software are closed. If

Page 9 of 14

Application Bulletin 353

Installation Instructions of TitrIC pro I

instrument, but it is recommendable to use the USB hubs of your computer, as they are more powerful. Then connect the two converters using the RS232 cable (6.2134.040). In the Device Manager of your Windows operating system check which two RS232 ports you are using.

Open the MagIC Net software. On the left click on

Configuration’, then in the devices window select

‘

Edit/New/Miscellaneous’ and choose the RS 232 device.

‘ Keep the proposed Device name and enter a random serial number. Go to the RS232 subsection and choose one of the used RS232 ports. Click on ‘

Connect’ and confirm with <Ok>.

Close the window with <Ok>

Repeat the same steps in the tiamoTM software and choose the second RS232 port.

7 Complete Analysis with TitrIC

As an example a short description of a complete analysis with TitrIC pro I is described here.

The whole procedure is controlled by the MagIC Net software which is the master software. In tiamoTM, the method TitrIC pro I is selected as a single determination. Start the determination in an autorepeat mode. To set it into autorepeat go to the Run window, right-mouse-click and select ‘

Autorepeat determination’. Close the window with <Ok>.

‘ Start the determination. Now tiamo

Properties Run window’. Under Process select

TM

is waiting for the start

signal from MagIC Net via RS232.

In the MagIC Net software select the method

TitrIC pro I.

Enter the required sample position, sample identification etc. into MagIC Net. For value 1 choose according to the list below:

 Value 1 = 1 a complete analysis including titration and

ion chromatography

 Value 1 = 2 only an ion chromatography analysis (e.g.

a calibration)

 Value 1 = 3 only a titration analysis

Start the determination in MagIC Net. All sample liquid handling is done by MagIC Net. At the right moment MagIC

TM

Net communicates to tiamo

via RS232 to do the titration analysis. Results are transferred back to MagIC Net and the rinsing steps of the titration equipment by MagIC Net initialized.

All data goes into the MagIC Net database and therefore one report can be generated.

Page 10 of 14

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Delivery p

Fo

the Dilution

ite

s:

N

mber

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1

8.

Installation

A

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de

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forward de

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t is

pplication Bulle

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re

oved. The

needle into

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swing head

mm.

in 353

ctions of TitrIC

xact position

he external indow manu

ngle is ~117

pro I

can be adju

ilution vesse

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sted by dire

with the ma

ximate valu

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out

8.

Dosino

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wit

sing Unit 10

Do

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pu

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mple Proces

8.

Transfer T

Th

10 mL Tr

unted on th

tra

sfer tubing t

ond end of

pi

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wh

ich is used b

External Di

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on

the right side

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bl dil

tion vessel

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Co

nnect the de

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(6. do

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Th

s gives the

p channels

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external di

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spiration tub

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nnected to

or.

bing

nsfer Tubin

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port 1 of th

the transfer

ng arm. If c

nd the M6 U

TitrIC pro I s

lution Vesse

ution vessel

of the 815 R nnected to t

B 2.

m ID PTFE pump tubing via Peristalti f the anions I

the rinse

ector outlet the MSM rin

etector wast ossibility to or pumping t

ution vessel

h is easiest

0 mL Dosin

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nted directly .

SB 1 at the

with holde

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ith all acces

botic USB S

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apillary (6.1

(6.1826.020

Pump of

. To be abl

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ith a 2x UN

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instead of

se one of th

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is mounted

when the ra

Unit is equip , included in

on the 2 L

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one end of

Dosing Unit.

ected to th

ove the 0.75

pling connec

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to connect he MSM to to use the

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the

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tion

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ter.

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ple

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Fi

.: Settings for t

8.

Analysis

Th

e following (.

t:

N



TitrIC pro I_

method. De following wi Value 1 = 1 ion chroma Value 1 = 2 a calibratio Value 1 = 3



This metho method in t



TitrIC pro I_

is used for to be used i

TitrIC pro I_



TitrIC pro I_

method is u HCl solutio combinatio

determinati



TitrIC pro I_

method is u Na2EDTA s combinatio

determinati

e 815 Robotic

met files) m

ilution:

This

pending on th

l be carried o

complete a

ography.

only an ion

)

only a titrati

is used in c

amoTM.

ilution condu

alibration of t

n combinatio

alibration co

iution titer de

ed to deter used in tiam with the tia

n of HCl.

iution titer de

ed to deter

lution used i with the tia

n of EDT

.

USB Sample

thods are pr

ethod is the

e value of

ut:

alysis includi

hromatogra

n analysis

mbination wi

ctivity calibrat

he conductivi

with the tia

ductivity

termination o

.

ine the titer o

. It has to b

oTM method

termination o

ine the titer o

tiamo. It ha

oTM method

rocessor XL

vided for M

standard

ue 1

the

g titration an

hy analysis (

h

TitrIC pro I

ion:

This met

y sensor. It h

oTM method

HCl:

This

the 0.1 mol/

used in

itrIC pro I_tit

EDTA:

This

the 0.05 mol

to be used i

itrIC pro I_tit

gIC

.g.

od

s

r

/L

r

Page 11

f 14

Application Bulletin 353

Installation Instructions of TitrIC pro I

The following methods (.mmet files) are provided for

TM

tiamo

:

 TitrIC pro I: This method is the standard method used. It

does the complete titration analysis and sends the results to MagIC Net. It is used in combination with

TitrIC pro I_dilution method in MagIC Net.

In tiamoTM it is typically used as a single determination in autorepeat mode. In other words, it is always ready to do a titration as soon MagIC requires it. To set it into autorepeat go to the Run window, right-mouse-click and

Properties Run window. Under Process select

select

Autorepeat determination. Close the window with <ok>.



TitrIC pro I_pH calibration manual: Here the iAquatrode

is taken out of the titration vessel and placed into the

TM

buffer solutions. Start this method only from tiamo



TitrIC pro I_titer determination of HCl: This method is

!

used for the titer determination. It has to be used in combination with the MagIC Net method

I_dilution titer determination of HCl

TitrIC pro

.

 TitrIC pro I_titer determination of EDTA: This method is

used for the titer determination. It has to be used in combination with the MagIC Net method

I_dilution titer determination of EDTA

TitrIC pro

.

 TitrIC pro I_conductivity calibration: This method is used

for calibration of the conductivity sensor. It has to be used in combination with the MagIC Net method

pro I_dilution calibration conductivity

.

TitrIC

With the TitrIC pro – Combo Kit Dilution following analysis can be carried out using the TitrIC pro I_dilution methods in MagIC respectively tiamoTM:

The whole procedure is controlled by the MagIC Net software which is the master software. In tiamoTM the method TitrIC pro I is selected as a single determination. Start the determination in autorepeat mode. To set it into autorepeat go to the Run window, right-mouse-click and select

Autorepeat determination. Close the window with <ok>. Start

Properties Run window. Under Process select

the determination. Now tiamoTM is waiting for the start signal from MagIC Net via RS232.

In the MagIC Net software select the method

I_dilution

. Enter the required sample position, sample

TitrIC pro

identification etc. into MagIC Net. For value 1 choose according to the list below:

 Value 1 = 1 complete analysis including titration and

ion chromatography.

 Value 1 = 2 only an ion chromatography analysis (e.g.

a calibration)

 Value 1 = 3 only a titration analysis

With Value 1 = 1 a complete analysis is carried out. Depending on the entered dilution factor the following will be done:

Dilution Factor = 1:

Depending on the measured conductivity an appropriate dilution factor is automatically applied by MagIC Net. The calculation factor is calculated according to Time Program No. 2. The finally used dilution factor is the integer of the conductivity divided by 250.

Dilution Factor >1:

If a dilution factor is known it can be directly entered into the sample table. It will be diluted accordingly. The conductivity measurement has no influence on the dilution in this case.

E.g. with Value 1 = 2 an ion chromatographic analysis will be carried out with the entered dilution factor. No dilution factor will be calculated

Page 12 of 14

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ctions of TitrIC

pro I

9

TitrIC pro

9.1

Delivery p

Fo

the Partial

foll

owing items:

N

umber Ar

MiPT

I

1

9.

Installation

A

hort straight

cribed here.

9.

Rinsing st

Fo

this applicat inner comp sample pro

porarily rem

dir

cting the ne trol (in the roximately r

er one app

les in the se

2.

The work p ternal positi

(e

ition 2). A

tion to drain t

Fig

.: Settings for t

9.

MiPT Dosi

Th

transfer c

do

ino on port

881. This in

pi

ce on the ne

Po

rt 4 of the D

wit

h the FEP A

Combo Kit

ckage

Loop setup

icle no. A

2.

00.0010 D

338.010 T

forward desc

tion

ion, a stoppe

rtment. The

essor, whic ved. The ex dle into the

window man

lates to a sw

oximately rel

tings for the

sitions are 1

n 1) and 60

ube is fixed

e waste wat

e 815 Robotic

o

pillary of 2

as well as ection valve

dle with a PE

sino with the spiration tub

artial Loop

ith TitrIC p

ticle designa

sino

trIC pro equi

ription of the

r is used to c

insing station

is easiest

ct position c

insing statio

ual). The in

ing head ang

ates to ~11

xternal needl

20 mm for

for the angl at the botto r.

USB Sample P

L is conne n the anion i is further co

EK capillary

2 mL Dosing (6.1829.010

o you need

ion

ment for MiP

partial loop k

lose the outl

is mounted

hen the rac

n be adjuste

with the ma

er compart

e of ~116.5°,

°. Define th e positions 1 he angle 11 e 111° (exte

of the rin

ocessor XL

ted both to

jection valv nected to th .5 mm i.d.

Unit is equip

, included in

the

it is

t of

nto

is by ual ent the

ese

and

.5°

rnal

ing

the

on

T-

ped

the

sing Unit 2

D pu

e water bottl

Th

e Dosino is

pr

Anion instru

9.

Analysis

Th

e following (.

N

t:

L) and mou

(6.1608.070

onnected to

ent.

met files) m

nted directly

).

SB 2 at the

thods are pr

on the 2 L

881 Compa

vided for M

ltra

t IC

gIC



TitrIC pro I_

Depending

iPT:

This me

n the value

thod is the st

f

Value 1

the

ndard metho

ollowing will

d.

e

carried out:

Value 1 = 1 ion chroma Value 1 = 2 a calibratio Value 1 = 3



This metho method in t



TitrIC pro I_

used for cal be used in

pro I_calibr



TitrIC pro I_

is used to d solution us with the tia

of HCl



TitrIC pro I_

method is u Na2EDTA s combinatio

determinati

complete a

ography.

only an ion

)

only a titrati

is used in c

amoTM.

iPT conducti

ibration of th

ombination

tion conducti

iPT titer dete

termine the

d in tiamo. It

oTM method

.

iPT titer dete

ed to deter lution used i

with the tia

n of EDTA.

alysis includi

hromatogra

n analysis

mbination wi

ity calibratio

conductivity ith the tiamo

it

.

rmination of H

iter of the 0.1

has to be use

TitrIC pro I_tit

rmination of E

ine the titer o

tiamo. It ha

oTM method

g titration an

hy analysis (

h

TitrIC pro I

:

This metho

ensor. It has

method

Cl:

This meth

Tit

mol/L HCl

d in combinat

r determinati

DTA:

This

the 0.05 mol

to be used i

itrIC pro I_tit

.g.

is

to

IC

d

ion

n

/L

r

Th

e following

TM

tia



:

mo

TitrIC pro I: It does the results to M TitrIC pro I

methods (.

This method

omplete titra

gIC Net. It i

MiPT method

met files)

is the standar

ion analysis used in com in MagIC Ne

re provided

method us

nd sends the

ination with

.

for

d.

In tiamoTM in autorepe to do a titra autorepeat select Prop

utorepeat

it is typically

t mode. In ot

ion as soon

o to the Run

rties Run wi

etermination

sed as a sin

her words, it i

agIC require window, righ dow. Under

.

Close the wi

le determina

always rea it. To set it i

-mouse-click rocess selec dow with

<o

ion

y

to

and

>

.

Page 13

f 14

Application Bulletin 353

Installation Instructions of TitrIC pro I

 TitrIC pro I_pH calibration manual: Here the iAquatrode

is taken out of the titration vessel and placed into the

TM

buffer solutions. Start this method only from tiamo



TitrIC pro I_titer determination of HCl: This method is

!

used for the titer determination. It has to be used in combination with the MagIC Net method

I_MiPT titer determination of HCl

TitrIC pro I_titer determination of EDTA: This method is



.

TitrIC pro

used for the titer determination. It has to be used in combination with the MagIC Net method

I_MiPT titer determination of EDTA



TitrIC pro I_conductivity calibration: This method is used

.

TitrIC pro

for calibration of the conductivity sensor. It has to be used in combination with the MagIC Net method

pro I_MiPT calibration conductivity

.

TitrIC

Import: Click on

TM

tiamo

Import

), then select under File / Method manager… / Edit /

… Import the methods related to TitrIC pro I with the

Method at the left (in MagIC Net and

MiPT kit from the MagIC Net CD. You will find the methods in the folder example/TitrIC of the CD.

Note!

Alterations of these methods should only be carried out by a person at the administrator level and who is thoroughly

TM

familiar with tiamo

and MagIC Net

With the TitrIC pro – Combo Kit Partial Loop following analysis can be carried out using the TitrIC pro I_MiPT

TM

methods in MagIC respectively tiamo

:

The whole procedure is controlled by the MagIC Net software which is the master software. In tiamoTM the method

TitrIC pro I is selected as a single determination.

Start the determination in autorepeat mode. To set it into autorepeat go to the Run window, right-mouse-click and select

Autorepeat determination. Close the window with <ok>. Start

Properties Run window. Under Process select

the determination. Now tiamoTM is waiting for the start signal from MagIC Net via RS232.

In the MagIC Net software select the method

. Enter the required sample position, sample

I_MiPT

TitrIC pro

identification etc. into MagIC Net. For value 1 choose according to the list below:

 Value 1 = 1 complete analysis including titration and

ion chromatography.

 Value 1 = 2 only an ion chromatography analysis (e.g.

a calibration)

 Value 1 = 3 only a titration analysis

Page 14 of 14

Metrohm TitrIC pro I User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual