Leica TP1020 V2.1 English – 12/2000
Always keep this manual near the instrument!
Read carefully prior to operating the instrument!
1.Important information
Serial No. .....................................................................
Year of manufacture: .................................................
Country of origin: .... Federal Republic of Germany
The information, numerical data, notes and value
judgments contained in this manual represent the
current state of scientific knowledge and state-of-theart technology as we understand it following thorough
investigation in this field. We are under no obligation
to update the present manual periodically and on an
ongoing basis according to the latest technical
developments, nor to provide our customers with
additional copies, updates etc. of this manual.
For erroneous statements, drawings, technical
illustrations etc. contained in this manual we exclude
liability as far as permissible according to the national
legal system applicable in each individual case. In
particular, no liability whatsoever is accepted for any
financial loss or consequential damage caused by or
related to compliance with statements or other
information in this manual.
Statements, drawings, illustrations and other
information as regards contents or technical details of
the present manual are not to be considered as
warranted characteristics of our products. These are
determined only by the contract provisions agreed
between ourselves and our customers.
Leica Microsystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Leica reserves the right to change technical
specifications as well as manufacturing processes
without prior notice. Only in this way is it possible to
continuously improve the technology and
manufacturing techniques used in our products.
This document is protected under copyright laws. Any
copyrights of this document are retained by Leica
Microsystems Nussloch GmbH.
Any reproduction of text and illustrations (or of any
parts thereof) by means of print, photocopy, microfiche,
web cam or other methods – including any electronic
systems and media – requires express prior permission
in writing by Leica Microsystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at the back
of the instrument.
8.1.1 Checking the voltage selector setting............................................................................................. 22
8.1.2 Adjust the voltage selector ............................................................................................................... 23
8.1.3 Connecting the mains cable ............................................................................................................. 23
8.2Switching on the instrument ............................................................................................................. 24
8.3Setting the time ................................................................................................................................... 24
8.4Installing the accessories ................................................................................................................. 25
8.4.1 Installing the paraffin stations.......................................................................................................... 25
8.4.2 Connecting the paraffin stations...................................................................................................... 25
8.4.3 Fixing the paraffin station connection cables (instruments with fume control ...........................
system only) ......................................................................................................................................... 26
8.4.4 Installing the reagent stations.......................................................................................................... 26
9.Programming the instrument ............................................................................................................ 27
9.2.2 Selecting the program........................................................................................................................ 28
9.2.3 Selecting the number of baskets ..................................................................................................... 28
9.2.4 Selecting the starting station............................................................................................................ 28
9.2.7 Displaying total program duration ................................................................................................... 30
9.2.8 Leaving the programming mode....................................................................................................... 30
10.Preparations for tissue processing ............................................................................................... 31
10.1 Filling the reagent stations ................................................................................................................ 31
10.2 Filling the paraffin stations ................................................................................................................ 32
10.3 Inserting the tissue basket ................................................................................................................ 33
11.Operating the instrument in the manual processing mode ...................................................... 34
11.1 Lifting and lowering the tissue basket ............................................................................................ 34
11.2 Moving the tissue basket to the next station ................................................................................. 35
11.3 Activating the vacuum function ...........................................................................................................
(instruments with vacuum function only) ....................................................................................... 36
4
Instruction Manual V 2.1 – 12/2000
2.Table of contents
12.Operating the instrument in the automatic processing mode ................................................. 37
12.1 Starting a program.............................................................................................................................. 37
12.2 Editing and altering programs while a processing cycle is in progress ................................... 41
12.3 Display end of processing time ........................................................................................................ 41
12.4 Pausing a process .............................................................................................................................. 42
12.5 Resuming a paused process............................................................................................................. 42
12.6 Stopping or aborting a process ........................................................................................................ 43
12.7 End of an automatic process ............................................................................................................ 43
12.8 Remove the specimens...................................................................................................................... 44
12.9 Finishing your daily work .................................................................................................................. 44
13.Warning and error codes - troubleshooting ............................................................................. 45
15.3 Replacing the fuses............................................................................................................................ 55
• When transporting the instrument, do not
lift it up by the carousel cover!
• This instrument may not be operated in
hazardous locations!
• Attention: the voltage selector is factory
preset.
Before connecting the instrument to the
mains make sure the correct setting matching your laboratory’s power supply has
been selected.
The socket for connecting the mains cable,
located at the rear of the instrument is
sealed with an adhesive tape which indicates the factory preset voltage for your
particular instrument.
• Connecting the instrument to the mains
with the voltage selector set at a wrong
value can cause severe damage to the instrument!
• When adjusting the voltage selector setting, the instrument must be disconnected
from the mains.
• The instrument may only be connected to
the mains with the cable supplied together
with the instrument and it may only be
connected to grounded sockets.
Operating the instrument
• The carousel may not be rotated manually!
Severe damage will result from doing so!
• Caution when lowering the carousel! Keep
your fingers out of the space between the
container lid and the upper rim of the container!
• Caution! In case of a power failure, the
carousel automatically descends into a
station.
• Instruments equipped with vacuum function may only be operated with the aluminium containers supplied together with the
instrument.
• While operating the instrument, no liquid
may enter in contact with any of the electrical connections or the interior of the instrument.
• Make sure to observe the level indicators
on the reagent and paraffin stations.
• Warning! Use caution when handling solvents! Make sure the premises are adequately ventilated! Explosion hazard!
• Spilled reagents have to be wiped away
immediately. In case of long-term exposure, the instrument surfaces are only conditionally resistant to solvents.
• Always observe worker’s protection rules
and use adequate protective gear (gloves,
laboratory coats).
• The heated wax baths may only be used
with paraffin. Under no circumstances may
they be filled with solvents. When solvents
heat, a highly explosive mixture builds up!
• Caution! The interior containers of the par
affin stations become very hot when the
heating function is activated! Do not touch
the gray upper rim of the containers with
your hands! Risk of injury!
• Caution when handling hot paraffin! Risk
of injury!
6
Instruction Manual V 2.1 – 12/2000
3.Safety instructions
Cleaning
• Before cleaning the instrument,
disconnect the mains switch.
• Caution! The interior containers of the paraffin stations become very hot when the
heating function is activated!
Do not touch the gray upper rim of the
containers with your hands! Risk of injury!
• Caution when handling hot paraffin! Risk
of injury!
• When cleaning the instrument, no liquid
may enter in contact with any of the electrical connections or the interior of the instrument.
• Spilled reagents have to be wiped away
immediately. In case of long-term exposure, the instrument surfaces are only conditionally resistant to solvents.
• To clean the painted surfaces, the container platform and the control panel, do
not use solvents containing acetone or xylene; neither use abrasive cleaning powders!
Only mild household detergents may be
used!
The lacquered surfaces and the control
panel are not resistant to xylene or
acetone!
Maintenance
• For purposes of maintenance or repair, the
instrument may only be opened by service
technicians authorized by Leica.
• Before exchanging the fuses, switch off the
mains switch and unplug the instrument!
• Burnt-out fuses may only be replaced by
fuses of the same type and specification.
For appropriate brands and specifications,
see Chapter 4 ‘Technical Data’.
Paraffin stations
• If the excess temperature repeatedly please call Leica Technical Service. DO
NOT continue to use the paraffin station.
• Check if the paraffin station works troublefree.
Use of defective paraffin stations has to
be discontinued for safety reasons!
Activated carbon filter (optional accessory)
• Warning! Fire hazard! It is important that
the activated carbon filters are changed
at factory recommended intervals. If a filter
becomes saturated with solvent, there is
a potential fire risk!
Leica TP1020 – Automatic Tissue Processor
7
4.Technical data
TypeTP1020
ApprovalsUL / cUL / VDE
Electrical data
Nominal voltage100 / 120 / 230 /240 V AC ±10%
Rate frequency50 - 60 Hz
Mains fuses (type MDA; manufacturer: Bussmann)2 x T 10 A, UL-approved
Nominal capacity1000 VA
Classifications according to IEC-1010/EN61010-1:
Protective classI
Pollution degree2
Overvoltage installation categoryII
Heat emission (max.)700 J/s
Working temperature range:5 °C - 40 °C
Relative humidity of air80%, non-precipitating
Dimensions
Carousel lid820 mm Ø
Height595 - 780 mm
Pitch diameter of rollers610 mm Ø
Weight
Net weight (including accessories)60 kg
Net weight, including packaging material116 kg
Paraffin stations
Quantity:2 (optionally 3)
Capacity:1.8 l
Nominal voltage / rated frequency:230 V AC, 50-60 Hz
Nominal capacity per station:150 VA
Temperature setting range:45 °C - 65 °C ±3 K
Excess temperature disconnection:85 °C ±5 K
Reagent stations
Quantity:10 (9 when 3rd paraffin station is installed)
Capacity:1.8 l
Quantity:9, freely selectable
Programmable infiltration time per station:up to 99 hours 59 minutes
Delayed start time of processing:up to 9 days
Dripping time:60 seconds
Vacuum function (types 2 and 4)
Pressure difference500 hPa maximum (appr. 0.5 bar)
8
Instruction Manual V 2.1 – 12/2000
5.General description
5.1Description of the instrument
The Leica TP1020 is an automatic tissue processor used
for the fixation, dehydration and infiltration of
histological tissue samples with fixatives, alcohol,
solvents and paraffin wax.
The reagent stations numbered 1 - 10 are used to
contain reagents. Station 10 may be replaced with an
optional third paraffin wax bath. Stations 11, 12, and if
used 10, are heated, temperature controlled wax baths
that can be filled with either wax pellets or molten
paraffin wax.
Embedding cassettes used to hold the tissue samples,
are placed into the tissue basket. The basic instrument
is designed for a single tissue basket. An optional
second basket can be added. The basket, or baskets,
are moved clockwise from station to station.
To ensure thorough infiltration the basket containing
the tissue samples is agitated, by moving up and down,
at each station. This function can be switched off at
any time.
During processing as the tissue basket moves from
station to station there is a delay period of sixty (60)
seconds during which time the basket is suspended
above the station. This ensures that there is minimal
reagent carryover from station to station.
All instrument functions are activated through the
control panel. Real time is displayed via LCD. The
instrument can be operated in manual and automatic
processing mode. Automatic processing is controlled
via 9 different programs which can be individually set
up, altered and edited.
5.2Standard delivery
The Leica TP1020 is available in 4 different versions:
Type 1-Basic instrument
Type 2-Basic instrument with vacuum function
Type 3-Basic instrument with fume control sy-
stem
Type 4-Basic instrument with vacuum function
and fume control system
The basic instrument comes with the following accessories:
-10 glass container with holders
-2 replacement glass containers
-2 paraffin containers
-1 standard tissue basket
-1 bag of Jet Cassettes without lids
-1 bag of lids for Jet Cassettes
-1 set of replacement fuses
-1 set of mains cables
-1 screwdriver
-1 crank handle (fixed in a clip on the housing)
-1 packing list
- 1 instruction manual in 4 languages (German,
English, French, Spanish)
Types 2 and 4 - instead of glass containers, these
instruments are supplied with
-10 aluminum containers with holders
Types 3 and 4 include in addition
-1 hose clamp
Vacuum function and fume control system are not
available as a retrofitting option.
If a power failure occurs, the specimens are protected
from drying out - even when overnight processing has
been selected, since in case of a power failure the
tissue basket will always be immersed into a station.
Once mains power is restored, processing will be
resumed where it had been interrupted. After a longterm power failure, critical excess immersion time in a
station will be visually displayed.
The Leica TP1020 has been designed to comply with
the strict UL and VDE safety regulations. It is
manufactured under a quality system in compliance
with ISO9001.
Leica TP1020 – Automatic Tissue Processor
An option available on a retrofitting basis is the station
holder for a second tissue basket (see Chapter 17,
‘Ordering Information’).
All types can also be retrofitted with a third paraffin
bath at station 10 (optional accessory).
You will find the standard accessories and - in case
you ordered them - further parts in the upper portion
of the packing box.
Compare the delivered parts with the packing list and
your actual order.
If you find any discrepancies, please contact your local
Leica sales organization immediately.
9
5.General description
5.3General overview Leica TP1020 - Type 1 with additional paraffin station (optional accessory)
Carousel cover
Reagent station lid
Glass container with
holder
Container station holder with
level indicator
Container station platform
Fig. 10.1
Tissue basket holder
Standard
tissue basket
Paraffin station
Notch for paraffin
station connection
cable
Control panel
Voltage selector
Mains switch
Socket for mains cable
Fig. 10.2 - TP 1020 - rear side
10
12
11
10
(socket of station no. 10
Instruction Manual V 2.1 – 12/2000
Sockets for
paraffin station
connector plugs
closed
with a cover cap
(not shown))
Paraffin station
connector plug
5.4General overview Leica TP1020 - Type 3
Plexiglass fume
containment
shield
5.General description
Fig. 11.1
Hose clamp
Fume outlet tube
(optional
accessory)
Activated carbon filter
receptacle
Fig. 11.2
Leica TP1020 – Automatic Tissue Processor
11
5.General description
Fig. 12.1
5.5Accessories supplied together with the
instrument
5.5.1 Standard tissue basket
Capacity: 80 cassettes
Tissue cassettes are loosely arranged in the basket.
5.5.2 Glass container with holder
Capacity:1.8 l
Reagent containers consist of a glass beaker inserted
into a metal holder with handle.
Fig. 12.2
Max
Min
Max
Min
Two marks in the metal holder serve as minimum and
maximum level indicators.
When filling the container the level of liquid should not
be below the minimum or above the maximum level
indication mark.
5.5.3 Aluminum container with holder
(standard outfit in instruments with
vacuum function or available as optional
accessory for other types)
Capacity:1.8 l
Instruments equipped with vacuum function are
supplied with aluminum containers instead of glass
containers.
In aluminum containers the level indications marks are
located on the inside of the container.
Fig. 12.3
12
When filling the container make sure the filling level is
not below the minimum or above the maximum level
indication mark.
Instruction Manual V 2.1 – 12/2000
5.General description
5.5.4 Paraffin station
Capacity:1.8 l
Nominal voltage:230 V AC
Rated frequency:50 - 60 Hz
Nominal capacity (heating):150 VA
2
5
4
1
Temperature setting range:45 - 65 °C ± 3 K
Excess temperature
switch-off mechanism:85 °C ± 5 K
If the actual temperature rises above the range
of normal working temperature, an excess
temperature switch-off mechanism responds.
The paraffin station heating is disconnected.
The yellow signal lamp is extinguished.
Use of the paraffin station can be resumed only
after a cooling down period. For cooling down,
disconnect the paraffin station connector plug
from the socket at the rear of the instrument,
or switch off the main switch.
The paraffin stations are equipped with heating. While
the heating is functioning, the yellow pilot lamp lights.
Fig. 13
The handle (2) is insulated. The paraffin container (3)
is also insulate to ensure that there is no risk of injury.
Caution! The interior containers of the paraffin
stations become very hot when the heating
function is activated! Do not touch the gray
upper rim of the containers with your hands!
3
Risk of injury!
The inside of the interior container (4) is coated with
Teflon. The interior container gets very hot. It has
minimum and maximum level indicator marks (5).
If the excess temperature switch-off
mechanism reacts several times, call
Technical Service without delay. In those
cases, use of the paraffin station in question
has to be discontinued immediately!
Leica TP1020 – Automatic Tissue Processor
13
6.The user interface
Fig. 14
Control panel
The control panel is an easy-care key pad.
0 4
Tissue basket
Processing station
0 9 : 1 7
The individual keys are organized in four functional
groups. Pressing any key will be acknowledged by an
audible signal. This function can be disconnected if
required (see Chapter 18).
Display
The display is a two-line LCD indication, each line with
16 single characters. When the instrument is on, the
display is always illuminated.
The display will indicate in which station the tissue
basket is located. At the same time the display also
reads real time (24 hour clock). The colon between
hours and minutes is blinking.
The display shows all tissue processing parameters
as well as the individual programs. Tissue baskets and
processing station are displayed with symbols.
In addition to programming features other useful data
is displayed; such as program duration and end of
processing. Also displayed are warning codes (W:01 W:06) to ensure trouble-free processing, and error
codes (E:01 - E:03) which indicate instrument or data
entry errors.
14
Instruction Manual V 2.1 – 12/2000
6.The user interface
Key functions
Programming, control panel locking, time setting,
up-and-down movement of the tissue basket
Programming button ‘PROG’
To call and quit the programming mode, in which
programs are set up, edited and altered, and to display
a program which is currently activated.
Lock button ‘KEY’.
To lock all control panel functions as protection against
accidental changes of programmed parameters.
0 4
L O C K E D
0 4
•To activate and quit the lock function, press ‘KEY’
for 5 seconds.
The display reads ‘LOCKED’.
1 0 : 2 0
After 10 seconds the display returns to standard
reading.
All key functions with the exception of the locking key
itself are now locked. Non of the key functions can be
activated. When pressing any key, the display will
always read ‘LOCKED’ for 10 seconds.
•To unlock the key functions, press ‘KEY’ again for 5
seconds.
All key functions will return to normal.
‘CLOCK’ symbol button
To set the time and to indicate the total duration, start
time and expected end of automatic tissue processing
programs.
Leica TP1020 – Automatic Tissue Processor
The display differentiates between indication of time
(real time, start time when working with delayed
starting function, end time) - which is indicated through
hyphens and colon - and indication of duration (holding
time of the tissue basket in a particular station, total
duration of process) - which is indicated through the
letters ´d´ and ´h´.
15
6.The user interface
‘THREE ARROW’ button for carousel up and
down movement
The ‘THREE ARROW’ button is used to start and stop
the carousel (tissue basket) up and down movement.
When this function is activated, the tissue basket is
lifted and lowered in three-second intervals within the
processing stations. This ensures a uniform mixing of
all liquids and an optimized tissue infiltration.
This function is automatically activated when a an
automatic processing cycle is started.
It can be switched off and back on at any time, also
when no processing cycle is running.
With the function being activated, the green diode in
the ‘THREE ARROW’ button is lighted.
•To switch off the function, press the ‘THREE ARROW’ button.
The diode is extinguished; the function is switched off.
It can, however, be turned back on at any time.
•To reactivate the function press the ‘THREE ARROW’ button once again.
If the tissue basket is located in a paraffin bath
that contains solid paraffin, the instrument
disconnects the up/down function
automatically.
16
Instruction Manual V 2.1 – 12/2000
0 1 V A C 0 h 1 2
P 4 P A U S E
6.The user interface
Start, pause, resume, stop and abort automatic
tissue processing cycles
'START' button
To immediately start processing or to insert delayed
starting parameters for automatic processing after
having selected a program.
‘PAUSE/CONT’ button
To pause and resume an automatic processing cycle.
When the function is activated, the display reads
‘PAUSE’. In addition, 5 minutes after last pressing a
button, a double sound signal will remind the user that
processing is still paused. This signal will repeat itself
every 5 minutes until the automatic processing cycle
is resumed.
When automatic processing is paused, the buttons for
manual processing become functional, so that e.g. the
tissue basket can be lifted out of the station to add tissue samples or to unload them for further/special processing.
0 1 V A C 0 h 5 5
P 8 S T O P ?
The pause period is terminated by pressing the 'PAUSE/
CONT' button. The processing cycle is resumed and
the remaining immersion time for that station is completed without interruption.
‘STOP’ button
To immediately stop the carousel up and down movement (press once) or to abort a processing cycle in
progress (press twice).
The display reads ‘STOP?’.
Leica TP1020 – Automatic Tissue Processor
17
6.The user interface
Manual processing keys
These buttons are only functional in the manual processing mode an while and, while an automatic processing mode is paused, via the ‘PAUSE/CONT’ button.
While the carousel is moving, the display
reads ‘WAIT!” and two blinking arrows
indicate the direction of the movement. Only
when the carousel has completely come to a
halt, this indication will disappear from the
display.
‘CIRCLE ARROW’ button to rotate the carousel
To move the tissue basket to the next station. Rotation
is only clockwise and while the carousel is in the upper
end position.
‘ARROW UP’ and ‘ARROW DOWN’ button for vertical
carousel movement
Lift and lower respectively the carousel to withdraw
the basket from a processing station or insert a basket
into a processing station.
To halt an up or down movement while still in progress,
push one of the two buttons again. The movement will
stop immediately when pressing the button.
‘V’ button for vacuum function
(types 2 and 4)
To connect / disconnect the vacuum function in the
manual processing mode.
The Leica TP1020 is available with and without a vacuum function. The instrument versions without vacuum functions do have the
‘V’ button in the control panel; however it is
not functional and the letter ‘V’ is not
displayed. Of course a vacuum function
cannot be activated with the ‘V’ buttons in
those cases.
18
Instruction Manual V 2.1 – 12/2000
6.The user interface
Programming mode buttons
These buttons are used to enter or alter parameters
when programming.
These buttons are equipped with a repeat
function.
If one of the ARROW is pressed for a while,
the cursor jumps to the respectively next data
entry position.
If one of the PLUS / MINUS buttons is pressed
for a while, the displayed numerical value is
continuously increased or decreased.
ARROW LEFT / ARROW RIGHT buttons (cursor)
To move the cursor in the direction of the arrow to the
respectively next data entry position.
• When pressing one of the ARROW buttons, the
cursor jumps to the next data entry position in the
direction of the arrow.
The Leica TP1020 is available with and without a vacuum function. The instrument versions without vacuum functions do have the
‘V’ button in the control panel; however it is
not functional and the letter ‘V’ is not
displayed. Of course a vacuum function
cannot be activated with the ‘V’ buttons in
those cases.
•To switch lines press ARROW LEFT.
PLUS / MINUS buttons
To modify the parameter in the position where
the cursor is located.
•To increase the numerical value, press PLUS.
•To decrease the numerical value, press MINUS.
•To change the number of baskets from 1 to 2, press
PLUS.
•To change the number of baskets from 2 to 1, press
MINUS.
•To change the station number press PLUS or MINUS
respectively.
•To switch on the vacuum function (types 2 and 4)
push PLUS.
•To deactivate the vacuum function (types 2 and 4),
press MINUS.
Leica TP1020 – Automatic Tissue Processor
19
7.Unpacking and installation
7.1Unpacking
2
1
3
Check the packaging material for visible
damage. If obviously there is damage, please
contact your freight forwarder immediately.
•Loosen the 3 screws on the upper and lower ends
of each of the four sides of the transport box (1).
4
9
•Remove the wooden lid (2).
•Remove the sponge rubber mat (3).
5
•Remove the instruction manual and the packing list.
•Remove all accessories of the individual compart-
8
7
ments of the cardboard grid (4).
•Remove the cardboard grid (4).
•Remove the intermediate bottom (5).
•Take away the wooden crate (1) moving it upwards.
Fig. 20.1
Fig. 20.2
•Remove the wooden ledge (6) from the interior box
(7).
6
•Remove the interior box (7) in an upward movement.
When transporting the instrument, do not lift
it up by the carousel cover!
11
• To lift up the instrument take it by the reagent
container platform or by the lower end of the
housing (Fig. 20.2) and lift it from the base plate(8).
•Install the instrument on a stable laboratory bench.
Four rollers (12) under the instrument housing permit
rotating the instrument on the bench.
•Remove the sponge rubber transport safety device
(9) from under the carousel cover.
For further secure transport of the instrument
we recommend you keep the original
12
packaging material.
20
Instruction Manual V 2.1 – 12/2000
780 mm
820 mm
7.Unpacking and installation
7.2Requirements at the installation site
This instrument may not be operated in hazardous locations!
Because of the quantity of solvents used for
processing and the solvent concentrations
that build up, for the instrument versions
without fume control system (containment
shields and activated carbon filter) we recommend installation in the laboratory’s fume
hood.
The installation site has to fulfill the following
conditions:
-Stable and plane installation surface
-Minimum installation surface dimensions: 850 x 850
mm
-Stable ambient temperature of +5 °C to +40 °C.
-Relative humidity: 80 % maximum.
Fig. 21
Necessary conditions for electrical connection
- Grounded wall outlet near enough so it can be
reached with the mains cable supplied together
with the instrument.
If you wish to install the basket holder for a
second tissue basket, you should do that now.
For mounting instructions, see Chapter 16
‘Optional accessories’.
Leica TP1020 – Automatic Tissue Processor
21
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