Leica MM LIVE-DEAD User Manual

Leica MM Live/Dead powered by MetaMorph

Analysis Software Drop-in for Leica MM AF

• Precise cell staining quantitation

• Generate area and intensity measurements

• Adaptive Background Correction™ for improved cell quantitation

• Field and Cell-by-Cell data logging

®

The Leica MM Live Dead Application Module for the Leica MM AF
software is designed to automate accurate identication of cells
after staining with a variety of live/dead staining kits.

The software simultaneously reports the total number and percent­age of live and dead cells, depending on stains used. Measure­ments of intensity and area are on a wavelength-by-wavelength
or cell-by-cell basis. The module can detect a second stain as a
subpopulation of an entire cell population. It can also be used with
exclusive markers to detect two different populations.

The module utilizes Adaptive Background Correction adapting the
cell detection algorithm to the local intensity ranges between and
within cells to provide the most robust segmentation available.
This technique enables probe detection even with highly variable
background uorescence within a single image.

The simple interface minimizes setup efforts and at the same time
enables users to customize the settings and measurements to ob­tain the best possible results specic to the type of cells used in
the experiments.

Image acquisition

CHO cells. Top: control, bottom: 1 μM Stauro­sporine. Cells were incubated for ~24 hours and
apoptosis was induced by incubating different
concentrations of Staurosporine for 6 hours. As­say kit: Molecular Probes, Vybrant Assay Kit #7.

Easy Configuration for analysis

Five easy steps allow you to get started in min­utes:

1. Select the image of interest

2. Choose the staining population (dead, live or all)

3. Specify the stained area (nucleus, cytoplasm
or both)

4. Set the minimum and maximum cell width

5. Determine the intensity above local back­ground

6. Optionally choose the parameters for reporting

Interactive data display

Once the analysis is run, the Cellular Results
table allows you to interactively view individual
cells’ data. Clicking one or multiple cells in the
image highlights the data for the selected cell(s)
in the table.

Multi-parameter analysis

Choose the measurements to capture, includ­ing:

• Live, dead and total cell counts

• Percentage of live and dead cells

• Per-wavelength average measurements

• Per-wavelength per-cell measurements

Powerful data export capabilities

All measurements can be directly exported to a
text le or Microsoft

®

Excel® for further analy-

sis.

“MetaMorph® is a Registered Trademark of MDS

Analytical Technologies”

Cell segmentation and analysis

The Live Dead module identifies live and dead
cells simultaneously

Data exporting

Measurements can be exported directly to a
spreadsheet program for further analysis.

Customization through journaling

Journals are sophisticated and powerful macros
that record and perform a series of tasks without
the need for a programming language. The mod­ules can be incorporated into a Leica MM AF
journal to increase the customization and auto­mation of your analysis.

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