Leica MM ANGIOGENESIS User Manual

Leica MM Angiogenesis powered by MetaMorph

Analysis Software Drop-in for Leica MM AF

• Unique z-Series acquisition

• Best focus algorithm for better quality result

• Adaptive Background Correction™ for improved segmentation

• Multi-Parameter analysis

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In many serious diseases, the human body loses control over an­giogenesis. Angiogenesis dependent diseases – such as cancer,
diabetic retinopathy, psoriasis, coronary artery disease, stroke
and rheumatoid arthritis – result when new blood vessels either
grow excessively or insufciently.

One of the morphological changes that can be observed in angio­genesis is tube formation. The in vitro tube formation experiment
poses special imaging challenges.

After plating, the cells grow into the matrix, with tubes developing
roughly parallel to the surface. However, the surface is not at and
some tubules grow perpendicular to it.

The Leica MM Angiogenesis Module for the Leica MM AF facili­tates the acquisition and analysis of tube formation experiments.

To capture the three dimensional behavior, the application module
allows for a unique z-series acquisition. A best focus image is then
obtained from the stack of images, and the analysis is run on that
image.

The module includes Adaptive Background Correction, adapting
the vessel detection algorithm to the local intensity ranges outside
the vessels to provide the most robust segmentation available.
This technique enables vessel detection even with highly variable
background uorescence within a single image.

The application module has a user-friendly interface to minimize
set up efforts and provide the exibility to customize the settings
and measurements collected to better suit your needs.

Unique 3D acquisition, Angiogenesis Endothe­lial Cell Tube Formation Assay

Courtesy of Min Wu, BD Biosciences.
Raw z-series images acquired.

Simple acquisition set up

1. Load an acquisition protocol

2. Choose appropriate settings

Easy configuration for analysis

1. Select the image of interest

2. Specify a range of tube widths

3. Select the contrast level

Multi-parameter analysis

Measurement options include:

• Total tube length

• Total tube area

• Mean tube thickness

• Number of segments

• Number of branch points

• Number of nodes

• Total area of nodes

• Mean node area

Best focus algorithm

Calculate best focus image.

image analysis

Best focus image is analyzed. White: tubes,
green: nodes.

Customization through journaling

Journals are sophisticated and powerful macros
that record and perform a series of tasks with­out the need for a programming language. The
Angiogenesis module can be incorporated into a
Leica MM AF journal to increase the customiza­tion and automation of your analysis

Powerful data export capabilities

All measurements can be directly exported to a
text le or Microsoft

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Excel® for further analy-

sis.

“MetaMorph® is a Registered Trademark of MDS

Analytical Technologies”

Data export for further analysis

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