Leica TILE SCAN, MARK AND FIND User Manual
Mark & Find
and Tile Scan
Motorized Stage Applications
LAS AF
Mark & Find and Tile Scan are part of the basic Leica Application Suite
for Advanced Fluorescence. When using a motorized stage, both modules are activated to give full advantage of a high precision stage over a
manual stage. Mark & Find enables the user to define multi position
experiments to observe a large number of cells within one experiment,
saving precious time. The Tile Scan feature allows the acquisition of
overview images of the specimen with multiple channels and in three
dimensions.
Mark & Find and Tile Scan are available on both the Leica AF6000
system series for widefield imaging and analysis and the Leica TCS SP5
confocal systems.
High precision positioning is even more important when working with
live cells. Preparing a live cell experiment not only takes time but also
occupies the resources of the laboratory such as incubators, clean
benches, etc. The experiment itself might run over hours or even several
days. During the course of the experiment the acquisition system is out
of use for other experiments. In the worst case scenario, the cells under
observation do not show the desired behavior or do not react to the
reagents added during the course of the experiment and the experiment
has to be repeated. Precious time and resources are lost.
Such situations can be avoided with the Leica Mark & Find feature
in combination with a high precision stage. These stages allow very
precise repositioning of previously defined locations. Multiple positions, for example multiple cells in the same Petri dish, can be stored
in advance of performing the experiment.
Motorized Stage Applications –
Mark & Find and Tile Scan
Mark & Find
Fig. 1: Mark & Find interface: Perform multi positioning
experiments and define location specific stack sizes.
Leica_Mark_&_Find 23.09.2005 10:00 Uhr Seite 1
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