Leica DM IRB User Manual

Leica DM IRB

Instructions

Issued in 1998 by:

Leica Microsystems Wetzlar GmbH

Ernst-Leitz-Strasse

D-35578 Wetzlar (Germany)

Responsible for contents:

Marketing MQM, product management, Tel. +49 (0) 6441-292519

Fax +49 (0) 64 41-29 2255

Leica DM IRB

Instructions

Copyrights

All rights to this documentation and the software it describes are owned by Leica Microsystems Wetzlar GmbH. Copying of text and illustrations – in full or in part – by printing, photostat, microfilm or other techniques, including electronic systems, is only permitted subject to the express written consent of Leica Microsystems Wetzlar GmbH.

The information contained in the following documentation represents the latest stage of technology and knowledge. We have composed the texts and illustrations with great care. However, as it is impossible to eliminate the risk of error completely, we cannot accept any kind of liability for the correctness of the contents of this manual. Nevertheless, we are always grateful to be notified of any errors.

The information in this manual may be altered without prior notice.

Contents

Important notes on this manual ....................... 7

General safety information ............................... 8

Intended application .......................................... 10

The microscope and its components ............. 11

Key subassemblies ............................................. 11

Stands ................................................................... 12

Tube mount........................................................... 12

Tube ..................................................................... 12

Brightness adjustment ....................................... 13

Coarse and fine control ..................................... 13

Mains switch ....................................................... 13

Incident light fluorescence device .................. 13

Aperture diaphragm ........................................... 13

Condenser ............................................................ 13

Condenser height adjustment .......................... 13

Specimen stages and accessories ................. 14

Objective nosepiece and objectives ............... 14

IR/R tube adapter ................................................ 14

Eyepieces ............................................................. 14

Transmitted light illumination unit ................... 14

Field diaphragm ................................................... 14

Lamphousings ...................................................... 14

Filters .................................................................... 14

Installation site ................................................... 15

Unpacking ............................................................ 16

Installation ........................................................... 16

Assembly .............................................................. 17

Assembly tools .................................................... 17

Assembly of the

transmitted light illumination column.............. 17

Assembly of condensers ................................... 18

Assembly of IC condenser prisms ................... 21

Condenser top ..................................................... 22

Condenser 0.30 S70............................................. 22

Condenser 0.53 S23 and 0.90 S1 ....................... 23

Assembly of field diaphragm ............................ 24

Assembly of filters and filter holder ................ 24

Assembly of ICT objective prisms,

IC module and IC objective prisms .................. 25

Differences between prisms D and D1 ........... 26

Inserting the analyser/polariser ...................... 26

Inserting the fluorescence module ................. 27

Assembly of the lamp mount, mirror housing, lamphousing,

illumination telescope ........................................ 27

Assembling and exchanging

incident light lamps ............................................ 29

Lamphousing 107 L.............................................. 29

Lamphousing 106 zL ........................................... 30

Assembling and exchanging

Hg and Xe lamps ................................................. 31

Assembly of the tubes and

tube adapter IR/R ................................................ 33

Adaption of the slide overlay device

and the macro dual system ............................... 36

Inserting the eyepieces/graticules ................. 37

Inserting the photoeyepieces ........................... 38

Screwing objectives in and out........................ 38

Assembling the stages, the plane stage

and object guide.................................................. 38

The E version DM IRB/E ..................................... 41

Contents

Learn mode .......................................................... 43

Installing the objective prisms ......................... 43

Learning the IC objective prisms ..................... 43

Installing the objectives .................................... 44

Parfocality ............................................................ 47

Dry objectives ...................................................... 47

Oil immersion objectives ................................... 47

Exiting the Learn mode ...................................... 47

Individual user adjustments .............................. 48

Installing the fluorescence filter cube ........... 49

Concluding the installation ............................... 49

Motorized objective nosepiece........................ 50

Operating modes................................................. 51

DRY and IMMERSION ........................................ 51

Changing the operating mode .......................... 51

Automatic lowering of the

objective nosepiece ........................................... 52

Brightness adjustment ...................................... 53

Electronic focus .................................................. 54

Operation .............................................................. 64

Operation

of transmitted light interference contrast ..... 78

Operation of incident light fluorescence ...... 81

Operation of filters ............................................. 88

Operation of the slide overlay device ............ 89

Operation of the macro device ........................ 90

Operation of LMC ................................................ 97

Principle of LMC ................................................. 98

Components ......................................................... 99

Assembly/adjustment ........................................ 100

Areas of application .......................................... 102

Care and maintenance ...................................... 103

Troubleshooting .................................................. 105

Basic setting for transmitted light .................. 64

Operation of objectives ..................................... 70

Operation of transmitted light.......................... 71

Operation of phase contrast ............................. 74

Operation of transmitted light darkfield ........ 76

Operation of transmitted light polarisation .. 77

Storage.................................................................. 112

Packaging and transport................................... 112

Technical description ........................................ 113

General technical data...................................... 125

Main wearing and spare parts, tools ............. 127

EU Conformity declaration ............................... 128

Important notes on this manual

This manual is an integral part of the Leica DM IRB microscope and must be read carefully before you start using the microscope.

This manual contains important instructions and information on the operating safety and maintenance of the system. It must therefore be kept in a safe place.

Text symbols and their meaning:

(1.2)

→ p. 20

The manual is multi-lingual. Due to the spiral binding you can turn the language version you want to the front.

The DM IR is available both as a life sciences microscope and as a metallographic/industrial microscope. In cases where the function and operation are identical, the same text and illustrations are used in both the separate instruction manuals.

Numbers in brackets, e. g. (1.2) refer to illustrations, in this example Fig. 1, item 2.

Numbers with an arrow, e.g. → p. 20 refer to a

particular page in this manual.

Special safety information is indicated by the triangular symbol on the left and is given a grey background.

Caution! Operation errors can damage the

microscope and/or its accessories.

Warning of hot surface.

Explanatory note.

Not part of all configurations.

General safety information

This instrument of safety class 1 has been built and tested according to EN VDE 0411-1, safety standards for electrical measurement, control and laboratory equipment.

To keep the microscope in this safe condition, it is essential to note the advice and warnings given in this manual.

The mains plug must only be inserted into a grounded outlet.

If an extension cord is used, it must be grounded as well. Any interruption of the ground connector inside or outside the microscope or disconnecting the ground connector can make the microscope potentially dangerous. Intentional interruption is forbidden!

n. b.:

61 010-1/IEC

1010-1/

Make sure that only fuses of the specified type and rating are used as replacements. It is forbidden to use mended fuses or to shortcircuit the fuse holder.

The instruments and accessories described in this manual have been safety-tested and checked for possible hazards. Before modifying the instrument in any way or combining it with non-Leica products not dealt with in this manual, it is essential to consult the Leica agency for your area or the main factory in Wetzlar!

Any unauthorized alteration to the microscope or use for which it was not intended will automatically terminate any warranty claim.

n. b.:

Using the ground connection, any accessories connected to the microscope which have their own and/or a different power supply can be given the same ground conductor potential. Please consult our servicing personnel if you intend to connect units without a ground conductor.

n. b.:

The electric accessories of the microscope are not waterproof. If water gets inside them, it may cause electrical shock. Do not put the microscope and its accessories too near a water supply or anywhere else where water may get inside them.

Before changing fuses or lamps, always turn the mains switch off and disconnect the mains cable.

n. b.:

Protect the microscope from major temperature fluctuations. These may lead to condensation which can damage the electric and optical components.

Avoid skin contact when using immersion oil! Ask the supplier for a safety information sheet!

n. b.:

Intended application

The new DM IRB is the logical further development of the successful inverted research microscope from Leica. It is used for examinations of cells and tissue, for micromanipulation and microinjection techniques all the way through to microdissection or confocal microscopy. The DM IRB has universal application potential, incorporating all the contrasting techniques of brightfield, darkfield, phase contrast, DIC, fluorescence and Hoffmann modulation contrast, which are all easy to use and switch between. Variable illumination and imaging light paths, HCS optics, modular accessories and a wide range of peripherals make the large DM IRB research microscope from Leica a versatile and powerful product.

The microscope and its components

Key subassemblies

The following views of the whole microscope show and name important subassemblies of the microscope and its accessories.

Fig. 1 – 2 1 Binocular phototube, 2 Eyepiece adapter tube, 3 Eyepieces, 4 Tube mount (tube change interface), 5 Tube port for photo/TV

connection, 6 Beamsplitter switch, 7 Mains switch, 8 Brightness adjustment, 9 Lateral TV port, 10 Coaxial coarse and fine drive, 11 Fluorescence module, 12 ICT prism adjustment, 13 Sextuple objective nosepiece, 14 Centring buttons for incident light field diaphragm, 15 Field diaphragm adjustment, 16 Filters, 17 Aperture diaphragm adjustment, 18 Lamphousing mount (or mirrorhousing for two lamphousings), 19 Lamphousing, 20 Stage plate, 21 Analyser, 22 Tube lens module (Bertrand lens and magnification changer), 23 Switch rod for lateral TV port, 24 Transmitted light illumination column, 25 Condenser, 26 Transmitted light lamphousing, 27 Transmitted light field diaphragm, 28 SLR port, 29 Second lamphousing

Fig. 1 View from the right Fig. 2 View from the left

2 1 20 4

24 25

22 23

17 18

29 14

15 9

6 12

8 7

The stand

There are 5 basic versions of the DM IRB stand, which allow over 50 microscope variants to be configured.

These 5 basic versions are: – Manual or electronic stand – With or without integrated fluorescence axis – With or without SLR front port or bottom port – Lateral photo port 100 % or 80 % – With or without integrated magnification

changer

The variants and their components, differences and uses will be explained individually in this manual. The function and operation of all microscopy techniques and the necessary accessories for the Leica DM IRB will be described and explained in detail in the Opera-

tion section of this manual.

First of all, here is a general overview:

The stand with bottom port cannot be equipped with an SLR front port. This variant is only produced at a customer’s specific request.

The electronic stand additionally offers a motorised objective nosepiece, electronic focusing, IC objective prism coding, LCD display of microscope functions and (optional) motorised filter cube changer (RF4-mot module) with electric dark flap, control panel, etc. All the above-mentioned stands are also available as fluorescence stands with integrated fluorescence axis. All the fluorescence stands (including the manual versions) can be fitted with the RF4-mot module.

Tube mount

The interface between the stand and the tube is called tube mount or tube change interface. The tube mount is compatible with DM IR tubes and the IR/R tube adapter which allows the use of DM R tubes.

Stands

The basic stand has a photo port on the left for the adaption of: TV camera, SLR camera or photomicro system. The variants offered send either 100 % or 80 % of the light to this photo port.

Besides the lateral photo port, the SLR stand also has another port facing the front (front port*) which can be equipped with either an SLR camera or a TV camera with c-mount connection.

Tube

The tube, or its tube lens, produces the primary image together with the objective. DM IR tubes consist of a basic part, the binocular part and the tube change ring. The trinocular tube also has a photo/TV port. A switchable mirror either directs the light 100 % to the eyepieces or 100 % to the photo port, or splits it 50 % /50 %.

Brightness adjustment

Aperture diaphragm

A 12 V 100 W transformer is built into the stand for stepless regulation of brightness with the brightness control.

Coarse and fine control

The coarse and fine focus control allows fast and precise focusing of the microscope image. Focusing is done by a vertical movement of the objective nosepiece. The vertical movement range is 9 mm.

Mains switch

The mains switch is used for switching the microscope power supply on and off.

Incident light fluorescence device

The variant with incident light fluorescence device contains the integrated fluorescence axis and the lamp mount or a mirrorhousing for adaption of a second lamphousing. The fluorescence stand also comprises the fluorescence module which takes 4 filter cubes. This module is also available as a motorised variant under the name RF4-mot module (see Technical Description).

The aperture diaphragm determines the resolution, field depth and contrast of the microscope image. The best resolution is achieved when the apertures of the objectives and the condenser are roughly the same.

The aperture diaphragm in the illumination light path is not intended for adjusting the brightness of the image. This should only be done by turning the brightness adjustment knob or using the neutral light damping filter.

Condenser

The condenser is a lens system through which the light is collected and focused on the specimen underneath. The condenser is designed to utilise the numerical aperture of the objective.

Condenser height adjustment

The markings on the transmitted light illumination column indicate the height settings of the different condensers.

n. b.:

Specimen stages and accessories

Transmitted light illumination unit

The specimen stage supports the specimens that are to be examined through the microscope. Several options are available to accommodate the wide variety of specimens examined, such as object guides, extension plates, specimen clips, scanning stage, heating stage, etc.

Objective nosepiece and objectives

The objective nosepiece is used to hold the objectives. L objectives with long working distances, for example, are specially corrected to respect different thicknesses of vessel bases. All microscope objectives from magnification

2.5x to 100x can be used. All objectives from the DM L and DM R range with 25 mm thread are compatible. The performance data of Leica objectives can be found in the chapter “Technical Data; Performance Data” or on the relevant objective lists available from your Leica agency.

IR/R tube adapter

The tube adapter is used to adapt tubes from the DM R range.

The transmitted light illumination unit consists of the lamphousing and the transmitted light illumination column. The transmitted light lamphousing comprises a precentred 12 V 100 W halogen lamp and a filter module for three swing-in filters.

Field diaphragm

The field diaphragm is used to produce Koehler illumination.

Lamphousings

A variety of lamphousings are offered for the DM IRB (for halogen, mercury or xenon lamps). The description and area of application can be found in the operation section of this manual.

Filters

Filters are generally used to enhance the contrast of the specimen and are assembled in the illumination column. A variety of different filters are easily changed.

Eyepieces

A magnified, virtual image of the actual intermediate image produced by the objective is produced with the eyepieces. They act as a magnifier.

Installation site

The microscope should be used in a dust-free room which is free of oil and chemical fumes and extreme humidity. Also, the workplace should not be exposed to major temperature fluctuations, direct sunlight or vibrations. These may impair measurements or photographs of the microscope image.

Ambient conditions:

Temperature 10 –36 °C Relative humidity 0– 80 % to 30 °C

Microscopes in warm and humid climates need special care to prevent build-up of fungus. Further details are given in the chapters “Maintenance” and “Storage”.

n. b.:

Lamphousings* and power units* must be placed at least 10 cm away from the wall and from flammable objects.

Unpacking

Installation

Please compare the delivery carefully with the packing note, delivery note or invoice. We strongly recommend that you keep a copy of these documents with the manual, so that you have information on the time and scope of the delivery later when ordering more equipment or when the microscope is serviced. Make sure that no small parts are left in the packing material. Some of our packing material has symbols indicating environment-friendly recycling.

n. b.:

Keep the packing material for storage and transport of the microscope and its accessories.

Try to avoid touching the lens surfaces of the optics. Any fingermarks on the glass surfaces should be removed with a soft leather or linen cloth. Even small amounts of finger perspiration can attack the surfaces of optical instruments within a short time. Further information is given in the Maintenance and Cleaning chapters.

n. b.:

● First take all the components out of the transport and packing material.

● Put the basic DM IRB stand on a desk which has enough room for it.

On no account should the microscope be connected to the power socket yet!

n. b.:

Assembly

Assembly tools

Installation and assembly of the microscope should preferably be carried out together with a member of Leica sales or service staff. Only a few ordinary screwdrivers are required for assembly, and these are supplied with the microscope.

Fig. 3 Assembly tools 1 Cross-tip screwdriver*, 2 Hexagonal screwdriver, 3 mm, 3 Centring keys, 2 mm*, 4 Centring keys, 1.5 mm*, 5 Allen key, 3 mm*, 6 Allen key, 2.5 mm* (short version)

Assembly of the transmitted light illumination column

Wipe off the interface surface (4.3) with a dry cloth. Tilt the illumination column (4.1) slightly to the back and insert so that the pin (4.2) engages in the groove of the interface surface (4.4).

Erect the TL illumination column and secure with the 4 screws.

When screwing on the TL illumination column, do not hold onto it so that optimal alignment to the optical axis is guaranteed. The angle of tilt of the illumination column can be varied or clamped securely in the vertical position with the knurled screw (5.1).

The lamphousing for transmitted light illumination for 12 V 100 W halogen lamps with single-lens aspherical collector and heat protection filter is an integral part of the transmitted light illumination column. The halogen lamp is preassembled. The chapter on Troubleshooting includes a description of how to assemble and change halogen lamps.

The cable on the illumination column can then be connected to the 12 V 100 W socket on the back of the microscope stand.

Assembly of condensers

The technical description of the condensers can be found in the chapter “Technical description”. All condensers of the Leica DM IRB are equipped with a 6-position rotating disc (6.2 and

8.3) and can be individually fitted with the

corresponding annular diaphragms for phase contrast (PH), darkfield (DF) or IC prisms for TL interference contrast (ICT) (10). Usually the annular diaphragms are already inserted in the condenser disc in the factory, so you will not normally have to fit them yourself. The condenser disc (11.5) is removed from the condenser by slackening the screw (11.4) on the underneath of the condenser.

Fig. 4 Assembly of transmitted light illumination column 1 Transmitted light illumination column, 2 Pin of TL illu-

mination column, 3 Support surface, 4 Groove of support surface, 5 Drill holes for fixing screws

Fig. 5 Transmitted light illumination column, back view 1 Knurled screw for clamping the transmitted light illumi-

nation column

Insert the light rings for Phaco (identified by the code numbers 0, 1, 2, 3 and the intercept distance S of the corresponding condenser top, e. g. 2 S1) and the DF diaphragm (identified by D for darkfield and the intercept distance S of the corresponding condenser top, e. g. D S1, see table “Technical Description”) in the slots of the disc as follows:

– Slightly unscrew the two centring screws

(10.11) using the supplied centring key (12.1).

– Insert the diaphragms so that the mount fits

under the spring (10.3) of the slot.

– When the light rings are assembled, their

identification code must be visible i.e. pointing upwards (12.3, 12.4 and 12.5).

– Insert the light rings in the order 0, 1, 2, 3. The

DF diaphragm can only be inserted in a large hole.

– Using the centring keys, screw the centring

screws back in until they no longer protrude over the outer edge of the disc.

– Fit IC condenser prisms if appropriate (see

assembly of ICT objective prisms).

– Insert the plastic labels (10.7) in the disc

(12.2), allocating them to the corresponding diaphragms.

– Mark any empty holes with white labels.

Insert the disc into the condenser with notches (10.6) facing upwards – towards the aperture diaphragm (6.3 and 8.4) – and screw down (11.4).

Fig. 6 Condenser 0.30 S70 1

Condenser top 0.30 S70 (not for use with condenser base (8.1)), 2 Condenser disc, 3 Aperture diaphragm, 4 Filter holder, 5 Condenser clamp screw

Fig. 7

Condenser holder

Condenser slide changer, 2 Condenser centring screws,

3 Condenser height adjustment, 4 Condenser clamp screw, 5 Screw for clamping the condenser holder

Fig. 8 Condenser 0.53 S23 1 Condenser base, 2 Condenser top 0.53 S23 (inter-

changeable), 3 Condenser disc, 4 Aperture diaphragm, 5 Filter holder, 6 Dovetail guide

Fig. 9 Condenser tops for condenser base (8.1) 1 Condenser top 0.53 S23, 2 Condenser top 0.90 S1, 3 Condenser top P 1.40 OIL S1, 4 Spacer ring for assembling

9.2 and 9.3

Fig. 10 6-position condenser disc, empty 1 Condenser disc with slots for light rings and IC condenser

prisms, 2 Guide groove for IC condenser prisms (2 concealed, 3 Spring, 4 Holes for centring keys, 5 Spaces for label plates, 6 Notches, 7 Label plates, 8 Light ring for darkfield, 9 IC condenser prism with 2 guide cams, 10 Light ring for phase contrast, 11 Centring screws

groove is

Assembly of IC condenser prisms

The IC condenser prisms are assembled at the factory. The following steps are only necessary in case of a retrofit: Remove the condenser disc (11.5) by slackening the screw (11.4) on the underneath of the condenser. – Using the centring keys (12.1), slightly un-

screw the two centring screws (10.11).

– IC condenser prisms can only be inserted into

the large holes of the condenser disc which have guide grooves (10.2).

– Insert the IC condenser prisms in ascending

order, e. g. K1, K2 and so that the mount fits under the spring (10.3) in the slot and the 2 guide cams engage in the grooves of the condenser disc (10.2).

– When the prisms are inserted, their

identification code, e. g. K10, must be visible and pointing towards the

centre of the disc

(12.6 and 12.7).

– Using the centring keys, screw the centring

screws back in until they no longer protrude over the outer edge of the disc. The prism is adjusted with the left centring screw only (see operation of ICT). The right centring screw must never restrict the adjustment range.

– Assemble the light rings and DF diaphragm if

appropriate (see previous section).

– Insert the label plates (10.7) corresponding to

the relevant IC condenser prism. – Mark any empty holes with white labels. – Remove any finger marks or dust on the

prisms carefully. – Put the condenser disc back in the condenser

with the notches (10.6) facing upwards –

towards the aperture diaphragm (6.3 and 8.4).

Screw down the disc (11.4).

Fig. 11 Condenser 0.90 S1 (bottom up) 1 Condenser base, 2 Condenser top 0.90 S1, 3 Spacer ring, 4 Fixing screw for condenser disc, 5 Condenser disc

Fig. 12 6-position condenser disc, fully equipped 1 Centring keys for centring screws (in working position), 2 Label plates, 3, 4 Light rings for phase contrast, 5 Light ring for darkfield, 6, 7 IC condenser prisms, 8 Hole for brightfield

Condenser top

The base and top of condenser 0.30 S70 form a self-contained unit (Fig. 6). The condenser top 0.30 S70 (13.4) cannot be used with the condenser base (8.1). The condenser top 0.53 S23 (8.2 and 9.1) is screwed straight on to the condenser base (8.1). A spacer ring (9.4 and 11.3) must be used for assembling the condenser tops 0.90 S1 and P 1.40 OIL S1 (9.2 and 9.3).

Assembly of the condensers to the illumination column

Condenser 0.30 S70

Tilt the TL illumination column to the back (13.1). Insert the condenser 0.30 S70 (13.4) from below into the dovetail guide of the illumination column (13.2), with the condenser top pointing towards the microscope stage. Adjust the height of the condenser until its upper edge is flush with the condenser height marking S70 on the illumination column. Secure the condenser with the supplied hexagonal screwdriver. Erect the TL illumination column.

Fig. 13 Assembly of condenser 0.30 S70 1

Transmitted light illumination column (tilted), 2 Dovetail guide, 3 Condenser height markings S1, S23 and S70, 4 Condenser 0.30 S70, 5 Condenser clamp screw, 6 Field diaphragm clamp screw, 7 Transmitted light lamphousing

Fig. 14 Assembly of condenser 0.30 S70 1 Condenser 0.30 S70 in working position (upper edge of

condenser is flush with condenser height marking S70)

Condensers 0.53 S23 and 0.90 S1

With the illumination column tilted to the back, insert the condenser holder (15.4) into the dovetail guide of the illumination column from below (15.2). The condenser height adjustment should point to the left. Adjust the height of the condenser holder until its upper edge coincides with the condenser height marking S23 or S1 on the illumination column (16.1), depending on the condenser top used. Secure the condenser holder with the hexagonal screwdriver and clamp screw (15.5). Mount the base part of the condenser with the dovetail guide (8.6) to the slide change mechanism (7.1) of the condenser holder (17). The condenser top should point downwards and the aperture diaphragm control towards the front (17.3). Slacken the clamp screw (17.5) and push the condenser back as far as the stop. Retighten the clamp screw (17.5) slightly.

Fig. 15 Assembly of condenser holder 1 Transmitted light illumination column, 2 Dovetail guide, 3 Condenser height markings S1, S23 and S70, 4 Condenser holder, 5 Clamp screw for securing the condenser holder, 6 Clamp screw for field diaphragm module, 7 Transmitted light

lamphousing

Fig. 16 Assembly of condenser holder 1 Condenser holder in working position for condenser

0.53 S23 (upper edge of condenser holder coincides with condenser height marking S23)

Fig. 17 Assembly of 0.53 S23 condenser 1 Dovetail guide of the condenser, 2 Sliding condenser

changer, 3 Aperture diaphragm adjustment, 4 Condenser top

0.53 S23, 5 Condenser clamp screw

Assembly of field diaphragm

Assembly of filters and filter holder

To enable Koehler illumination when using condensers 0.53 S23 and 0.90 S1, a field diaphragm has to be assembled. Insert the field diaphragm module (18.1) into the mount (Fig. 18) from below. The diaphragm adjustment (18.2) should point in the direction of the tube. Secure with clamp screw (18.3).

Fig. 18␣ ␣ Assembly of field diaphragm 1 Field diaphragm module, 2 Field diaphragm adjustment, 3 Clamp screw for securing the field diaphragm module

1 2

The Leica DM IRB is equipped with a holder with spaces for 3 filters with 40 mm diameter. The filters are already fitted into the holder at the factory. If you are retrofitting filters yourself, assemble as follows:

● Slacken the clamp screws (Fig. 19.1) and

remove the filter holder.

● Put the filters into the holder (20).

● Mount the filter holder onto the transmitted

light illumination column and secure in position with the clamp screws.

Fig. 19 Assembly of filters 1 Clamp screw for securing the filter holder

Fig. 20 Assembly of filter holder for 3 filters

Assembling the ICT objective prisms

Assembling the IC module and IC objective prisms

The IC prism disc with the IC prisms ordered by the customer are already assembled in the microscope at the factory. In case you want to retrofit the IC prism disc, please proceed as follows:

Fig. 21 IC objective prism disc without fixing knurl 1 IC objective prism in mount, 2 Code letter (e. g. A), 3 Washer and screw, 4 Stop pin

Remove the front cover (22.2) under the objective nosepiece (23.1) after slackening the Allen screws (22.4). Insert the IC prism disc (22.1) in the mount and tighten with the two Allen screws. n. b.: insert the prism disc with the prism mount pointing downwards.

Retrofitting individual IC prisms:

Please align prisms against the stop pin (21.4) and only screw down lightly to avoid strain. Insert the prisms so that the code letter, e. g. A points upwards and is readable. Label the position of the prism on the front of the ICR prism disc with a label plate (22.5).

Examples of prisms:

Prism A – for objectives N PLAN 5x, 10x. Prisms D and D1 – both for objectives N PLAN 20x, 50x, 100x and HC PL FLUOTAR 5x – 100x.

Fig. 22 Assembly of IC objective prism module 1 IC objective prism module, 2 Cover, 3 Fixing screw, 4 Hole for fixing screws, 5 Label plates, 6 Knurled fixing knob

5 6

Abb. 23 Assembly of IC objective prism module 1 Objective nosepiece, 2 Mount for IC objective prism module, 3 Stop pins

Differences between prism D and D1

Inserting the analyser

Prism D is the standard prism with greater shearing and therefore higher detection sensitivity for minute topological and refractive index variations in the specimen. Prism D1 has smaller shearing than prism D and a lower detection sensitivity for topological and refractive index variations. However, prism D1 is better at resolving details of fine specimen structures.

Remove the blind slide and insert the analyser

(24.2) from the left as far as the 1

clickstop.

Inserting the polariser

The polariser is inserted into the filter holder of the condenser. In addition a whole-wave compensator is applied to the back of the polariser. It is activated by turning the polariser over, in order to enable colour contrasting in polarisation or interference contrast (the com-

pensator is active when the lambda symbol λ is

visible from above).

Fig. 24 Polariser/analyser 1 Polariser POL , 2 Analyser ICT ↔

↔

Fig. 25 Condenser 0.53 S23 1 Filter holder with polariser inserted (swung out)

Inserting the fluorescence module

The fluorescence module (Fig. 26) is part of the fluorescence stand, but is also available as a retrofit kit. To retrofit the fluorescence module, remove the blind cover from the stand. The fluorescence module can be fitted with up to four different filter cubes (26.3). They are inserted into the dovetail mount (26.2) of the fluorescence module with their engraving facing downwards (towards the turret plate). The fluorescence module is inserted on a dovetail guide into its slot on the stand by pushing it as far as the stop. One part of the fluorescence module is the anti-glare protection (27.1), which can be inserted between the tube and the stage. Proceed in the same way if you are inserting a motorised filter cube changer instead of the manual filter module. Also read the manual for the electronic version.

Assembly of the lamp mount, mirror housing, lamphousing, illumination telescope

1. Insert the lamp mount or mirror housing in the back panel and screw down with Allen screws. Engage the guide pin of the lamp mount (29.1) in the back panel of the microscope stand (28.2).

2. Hold the lamphousings 107/2, 107, 106 z against the lamphousing mount and secure with the fixing screw (Fig. 31).

3. We recommend using the illumination telescope for gas discharge lamps. This is inserted between the lamp mount and the lamphousing 106 z (30.4) and magnifies the image of the focal point of the lamp by the factor 2x in the entrance pupil of the objective. This results in a significantly higher illumination intensity for fluorescence.

Fig. 26 Fluorescence module 1 Rotatable turret, 2, 4 Dovetail mounts for filter cubes (the

numbers 1␣ –␣ 4 are markings of the assembly positions),

3 Filter cubes, 4 Display of the position in the light path, 5 Switch rod with BG 38 and light stop

Fig. 27 Anti-glare protection

4. Connect the lamp plug to the connecting socket in the stand (28.3).

5. Insert light filters, 50 mm Ø into the 2 spaces in the lamphousing mounts (29.4).

n. b.:

Connect the appliance cable to the mains socket on the microscope stand (28.4)!

Fig. 28 Back view of microscope 1 Space for assembling a lamphousing mount or mirror

housing, 2 Hole for guide pin, 3 Socket for lamp plug, 4 Mains socket, 5 Potential equalisation

Fig. 30 Mirror housing and illumination telescope 1 Lever for mirror switching, 2 Lateral lamphousing mount

with fixing screw, 3 Back lamphousing mount with Allen screw, 4 Illumination telescope for gas discharge lamps

3 2

5 4

Fig. 29 Lamp mount 1 Guide pin, 2 Lateral lamphousing mount, 3 Dovetail ring for

mounting to stand, 4 2 spaces for light filters, 5 Allen screws for fixing

Fig. 31 Lamphousing 106 z L 1 Collector adjustment, 2 Vertical lamp adjustment, 3 Horizon- tal lamp adjustment, 4 Mount ring

3 1

Assembling and exchanging incident light lamps

Exchanging the 12 V 100 W halogen lamp:

n. b.:

Disconnect the lamp and lamphousing from the power supply. Pull out the mains plug.

Fig. 32a –c Lamphousing 107 L 1 Mount with halogen lamp, 2 Collector

Lamphousing 107 L

Slacken the fixing screw on the cover and lift off the cover (Fig. 32a and 32b). Move the collector to the front and pull the defect 12 V 100 W lamp out of the base towards the front. Without removing its protective cover, put a new lamp into the base, without tilting, as far as it will go.

n. b.:

Leave the protective cover on the lamp until it is in position. Avoid making finger marks or wipe off immediately. Close the lamphousing.

Lamphousing 106 z L

Slacken the fixing screw on the lid (33.10). Pull the cut-out plug slightly out of the socket and flip up lid (33.11; 33.1). Move the collector to the front and lift the defect lamp out of the base (33.1; 33.2; 33.3). For convenience, the lamp holder can be removed from the lamphousing as well. To do this, slacken the fixing screws on the lamp holder (33.10) and pull out lamp holder (Fig. 34). Without removing its protective cover, put a new lamp into the base, without tilting, as far as it will go.

n. b.:

It is important to leave the protective cover on the lamp until it is in position. Avoid making finger marks or wipe off immediately. Close the lamphousing.

Fig. 33 Lamphousing 106 z L 1 Lid, flipped up, 2 Collector, 3 12 V 100 W halogen lamp or gas discharge lamp in holder, 4, 9 Cover fixing screws, 5 Reflector, 6, 7, 8 x-y adjustment screw for reflector, 10 Fixing screws for lamp mount, 11 Socket for contact plug

8 9

10 11

2 3

Fig. 34 12 V 100W lamp holder with halogen lamp

+ 100 hidden pages