LEICA DMI6000 B User Manual

Leica DMI6000 B

Operating Manual

1

Published October 2004 by:

Leica Microsystems Wetzlar GmbH
Ernst-Leitz-Straße
D-35578 Wetzlar (Germany)

Responsible for contents:
Bernard Kleine
(Marketing CM, Compound Microscopy, Product Manage­ment)
Holger Grasse
(Safety Officer according to MPG §30) In case of questions, please contact the hotline:

Phone [phone]
Fax [phone]
E-mail: [e-mail]

2

Leica DMI6000 B

Operating Manual

3

Copyrights

Copyrights

All rights to this documentation are held by
Leica Microsystems Wetzlar GmbH. Reproduc­tion of text or illustrations (in whole or in part) by
print, photocopy, microfilm or other method (in­cluding electronic systems) is not allowed with­out express written permission from Leica Mi­crosystems Wetzlar GmbH.

The term "Windows" may appear in the following
text without further identification. It is, however,
a registered trademark of Microsoft Corpora­tion. The names of companies and products
used herein may be trademarks of their respec­tive owners.

The instructions contained in the following doc­umentation reflect state-of-the-art technology
and knowledge standards. We have compiled
the texts and illustrations as accurately as pos­sible. Nevertheless, no liability of any kind may
be assumed for the accuracy of this manual’s
contents. Still, we are always grateful for com­ments and suggestions regarding potential mis­takes within this documentation.

The information in this manual is subject to modifi­cation at any time and without notification.

4

Contents

Contents

1. Important Notes about this Manual ...... 7

2. Intended Purpose of the Microscope ... 8

3. Safety Notes ............................................... 9

3.1 General Safety Notes ............................... 9

3.2 Electrical Safety ........................................ 10

4. Overview of the Leica DMI

4.1 Specifications ............................................ 12

4.2 Glossary....................................................... 16

5. Unpacking the Microscope .................... 22

6. Assembling the Microscope .................. 25

6.1 Assembly Tools .......................................... 25

6.2 Installation of the Transmitted Light

Illumination Carrier (DL) ........................... 26

6.3 Installation of the DIC Module

and DIC Objective Prisms ........................ 27

6.4 Installation of Specimen Stages............. 28

6.5 Installation of Condensers ....................... 34

6.6 Installation of Eyepieces .......................... 39

6.7 Installation of Objectives ......................... 39

6.8 Installation of Filters

in the Illumination Arm ............................. 40

6.9 Installation of the

Transmitted Light Lamp Housing ............ 40

6.10 Installation and Replacement of the

Transmitted Light Lamps: .........................

Lamp Housing 107 or 107/2 ...................... 41

6000 B......... 12

6.11 Installation of Lamp Housing Mount

and Mirror Housing ................................... 42

6.12 Installation and Replacement

of Incident Light Lamps ............................ 44

6.13 Equipping the Incident Light

Tu rret Disk ................................................... 48

6.14 Installation of the Polarizer and

Analyzer....................................................... 50

6.15 Optional Accessories ............................... 52

6.16 Connection to the

Electronics Box CTR6000 ......................... 53

6.17 Connection to the Computer ................... 54

6.18 Connection to the Power Supply ............ 54

7. Start-up........................................................ 55

7.1 Functional Principle .................................. 55

7.2 Switching on the Microscope ................. 59

7.3 The LeicaScreen........................................ 60

7.4 The Function Buttons on the Stand ....... 61

7.5 The SmartMove Remote Control

Module......................................................... 64

7.6 Illumination ................................................. 65

7.6.1 Transmitted Light ............................ 65

7.6.2 Incident Light - Fluorescence ...... 68

7.7 Checking Phase Contrast Rings ............. 69

7.8 Setting the Motorized Polarizer ............. 70

7.9 Adjusting the Light Sources .................... 71

5

Contents

8. Operation .................................................... 74

8.1 Switching On .............................................. 74

8.2. Contrast Methods ...................................... 76

8.2.1 Brightfield (TL)................................. 76

8.2.2 Phase Contrast (TL) ........................ 77

8.2.3 Darkfield (TL) ................................... 77

8.2.4 Polarization (TL) .............................. 78

8.2.5 Differential

Interference Contrast (TL) ............ 79

8.3 Fluorescence .............................................. 80

8.4 Combination Methods .............................. 81

8.5 Focusing ...................................................... 82

8.6 Tubes............................................................ 84

8.7 Eyepieces .................................................... 85

8.8 Objectives ................................................... 85

8.9 Stages and Object Displacement ........... 88

8.10 Magnification Changer............................. 89

8.11 Light Sources ............................................. 89

8.12 Aperture and

Field Diaphragm ......................................... 90

9. Troubleshooting......................................... 91

10. Care of the Microscope ........................... 95

10.1 Dust Cover .................................................. 95

10.2 Cleaning....................................................... 95

10.3 Handling Acids and Bases ...................... 96

11. Major Consumable and Replacement

Parts ............................................................ 97

12. Dimensions ................................................. 98

13. Abbreviations and Pictograms ............... 99

14. Index ............................................................ 101

15. EU Declaration of Conformity ................. 104

6

1. Important Notes about this Manual

1. Important Notes about this Manual

Caution!

This operating manual is an essential com­ponent of the microscope, and must be read
carefully before the microscope is assem­bled, put into operation or used.

Text symbols, pictograms and their meanings:

(1.2)

p.20

→

This operating manual contains important in­structions and information for the operational
safety and maintenance of the microscope and
accessories. It must therefore be kept safely for
future reference.
A separate manual is available on CD-ROM cov­ering the operation of the Leica Application
Suite (LAS).

Numbers in parentheses, such as "(1.2)", corre­spond to illustrations (in the example, Figure 1,
Item 2).

Numbers with pointer arrows (for example
→ p.20), point to a certain page of this manual.

Caution!
Special safety instructions within this manu­al are indicated with the triangle symbol
shown here, and have a gray background.

Caution! The microscope and accessories can

!

*

be damaged when operated incorrectly.

Explanatory note.

Item not contained in all configurations.

7

2. Intended Purpose of the Microscope

2. Intended Purpose of the Microscope

The Leica DMI 6000 B microscope described in
these instructions is intended for biological rou­tine and research applications. This includes the
examination of samples taken from the human
body to provide information on physiological or
pathological states or congenital abnormalities,
or determing the safety and compatibility with po­tential recipients, or monitoring therapeutic
measures.

The Leica DMI
of Leica’s proven inverted research micro­scopes. It is designed for cellular and tissue ex­amination, micromanipulation and microinjec­tion techniques, microdissection, and confocal
microscopy. The Leica DMI
universal deployment, all contrast methods such
as darkfield, brightfield, phase contrast, DIC, flu­orescence, and modulation contrast are integral
to the microscope and can be adapted or
changed quickly and easily. Variable illumination
and imaging beam paths, as well as HCS optics,
modular accessories and a comprehensive
range of peripherals, complement the Leica DMI
6000 B inverted research stand.

6000 B is a further development

6000 B is suitable for

The above-named microscope complies with
the Council Directive 98/79/EEC concerning in
vitro diagnostics. It also conforms to the Council
Directives 73/23/EEC concerning electrical ap­paratus and 89/336/EEC concerning electromag­netic compatibility for use in an industrial envi­ronment.

Caution!

The manufacturer assumes no liability for
damage caused by, or any risks arising from
using the microscope for other purposes
than those for which it is intended or not us­ing it within the specifications of Leica Mi­crosystems Wetzlar GmbH.
In such cases the declaration of conformity
shall cease to be valid.

Caution!

This (IVD) device is not intended for use in
the patient environment defined by DIN VDE
0100-710. Neither is it intended for combin­ing with medical instruments according to
EN 60601-1. If a microscope is electrically
connected to a medical instrument accord­ing to EN 60601-1, the requirements de­fined in EN 60601-1-1 shall apply.

8

3. Safety Notes

3. Safety Notes

3.1 General Safety Notes

This safety class 1 device is constructed and
tested in accordance with
EN 61010-2-101:2002,
EN 61010-1:2001,
IEC 1010-1:2001,
Safety regulations for electrical measuring, con­trol, and laboratory devices.

In order to maintain this condition and to en­sure safe operation, the user must follow the
instructions and warnings contained in this
operating manual.

Caution!

Caution!

The devices and accessories described in
this operating manual have been tested for
safety and potential hazards.
The responsible Leica affiliate or the main
plant in Wetzlar must be consulted whenev­er the device is altered, modified, or used in
conjunction with non-Leica components that
are outside of the scope of this manual.

Unauthorized alterations to the device or
noncompliant use shall void all rights to any
warranty claims!

9

3. Safety Notes

3.2 Electrical Safety
General specifications

Caution!

Leica CTR6000 Electronics Box

For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:

Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:

Microscope

For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:

ebq 100 Supply Unit*

90-250V~
50-60 Hz
max. 290VA
T6.3 A
(IEC 60127-2/3)
15-35°C
max. 80% to 30°C
II
2

90-250V~
50-60 Hz
See CTR6000
See CTR6000
15-35°C
max. 80% to 30°C
II
2

Power plugs may only be plugged into an
outlet equipped with a grounding contact.

Do not interfere with the grounding function
by using an extension cord without a ground
wire. Any interruption of the ground wire in­side or outside of the device, or release of
the ground wire connection, can cause the
device to become hazardous. Intentional
ground interruption is not permitted!

Caution!

Peripheral devices with their own or sepa­rate power supplies that are connected to
the microscope can have the same protec­tive conductor potential by connecting them
to the ground screw on the back of the Leica
CTR6000 electronics box. For connections
without a ground connector, Leica Service
must be consulted.

For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
(See enclosed manual)

10

90-250V~
50-60 Hz
max. 155VA
2xT2A (IEC 127)
10-36°C
max. 80% to 30°C
II
2

Caution!

Never use any fuses as replacements other
than those of the types and the current rat­ings listed here. Using patched fuses or
bridging the fuse holder is not permitted. The
use of incorrect fuses may result in a fire
hazard.

Caution!

The microscope’s electrical accessory com­ponents are not protected against water.
Water can cause electric shock.

Caution!

Protect the microscope from excessive tem­perature fluctuations. Such fluctuations can
lead to the accumulation of condensation,
which can damage the electrical and optical
components.
Ambient temperature: 15-35°C.

Caution!

3. Safety Notes

Before exchanging the fuses or lamps, be
absolutely certain to switch off the main
power switch and remove the power cable.

11

4. Overview of the Instrument

4. Overview of the Leica DMI 6000 B

4.1 Specifications

Contrast Methods

Transmitted Light Axis

Incident Light Axis

•Transmitted light (DL): BF, DF, PH, DIC, Pol

• Incident light (IL): Fluo

• Combination (DL/IL): Fluo/DIC, Fluo/PH

•Intermediate pupil: IMC (integrated modulation contrast)
IPH (integrated pos./neg. phase contrast)

• Automatic Illumination Manager

(aperture, field diaphragm, intensity, process switching)

• Automatic, color-neutral intensity control

• Manual or motorized shutter

• Lamp housing mount for interchangeable lamp housings.

• Automatic, electronic condenser identification

• Aautomatic Illumination Manager

(aperture, field diaphragm, intensity, process switching)

• Automatic, color-neutral intensity control

• Motorized shutter (switching speed < 50ms)

• Lamp housing mount for up to 3 interchangeable light sources

• Motorized 6-place filter turret

• Fluorescence Intensity Manager (FIM)
(reduction of incident illumination intensity)

• Mechanical booster lens for central boosting of
fluorescence or uniform distribution

• Motorized Excitation Manager to monitor fluorescence
emission when using double and triple filter cubes

• Ultrafast filter wheel for 3 excitation wavelengths
(switching speed < 50 ms)

12

Tube

• Ergonomic with or without camera port at left

•2 switching positions: 100%VIS and 50%VIS / 50%CAM

• Optional Bertrand lens

• Eye spacing adjustment

• Height and angle adjustment (10° - 45°)

4. Overview of the Instrument

Magnification Changer

Objective Turret

Stages

• Motorized

•3 switching positions
(choice of magnifications: 1x, 1.5x, 1.6x or 2.0x)

• Effective for all camera ports

or

• Manual

•2 switching positions
(choice of magnifications: 1x, 1.5x, 1.6x or 2.0x)

• Effective on tube port

• Motorized and coded

• 6x for objectives with M25 thread and 45mm parfocal distance

• For DIC: motorized/coded Wollaston prism carousel

• Anti-vibration locking

Fixed regular stages

• Ceramic-coated stage plate (248mm x 204mm)

•Heated stage plate (from 3°C above room temperature to 60°C)
(248 x 212mm)

•Temperature-controlled stage plate (0°C to 60°C) (248mm x
212mm)

Fixed micromanipulation stages

• Ceramic-coated stage plate (248mm x 204/122mm)

•Heated stage plate (from 3°C above room temperature to 60°C)
(248mm x 204/122mm)

•Temperature-controlled stage plate (0°C to 60°C)
(248mm x 204/122mm)

Regular manual 3-plate cross-stage

• Positioning range: 83mm x 127mm

• 20 optional inserts (standard, heating, cooling) for a variety
of applications, size of inserts:160mm x 110mm (compatible
with scanning stages)

Manual micromanipulation 3-plate cross-stage

• Positioning range: 40mm x 40mm

•3 optional inserts for a variety of applications

Motorized micromanipulation 3-plate cross-stage

• Positioning range: 40mm x 40mm

Scanning stage IM 120 x 100 (motors on top)

• 1mm, 2mm, 4mm spindle pitch
(higher resolution v. higher speed)

• 20 optional inserts (standard, heating, cooling) for a variety
of applications, size of inserts:160mm x 110mm

13

4. Overview of the Instrument

Scanning stage IM 120 x 100 (motors on bottom)

• 1mm, 2mm, 4mm spindle pitch
(higher resolution vs. higher speed)

• 20 optional inserts (standard, heating, cooling) for a variety
of applications, size of inserts:160mm x 110mm

Condensers

Z focus

Observation Ports

• Motorized and coded

• Motorized or manual aperture diaphragm

• Contrast methods: BF, DF, PH, DIC, Pol, IMC

• Automatic method switching

• Condenser turret with 7 positions for contrast methods

•2 condenser housings (S1-S28 and S70)

• Condenser heads: S1/1.4 oil, S1/0.9 dry, S23/0.53, S28/0.55

• Condenser heads can be swung out

• Condenser S70 with additional lens for low magnifications

• All condensers suitable for magnifications from 1.25x to 100x

• With or without motorized or manual polarizer

•With or without motorized or coded Wollaston prism disk

• Motorized and coded

• 9mm travel (1mm below, 8mm above the stage)

• Maximum travel speed: 5mm/s

•5 focus steps: 0.05 µm, 0.1 µm, 0.7 µm, 1.5 µm, 5.0 µm

• Electronic focus repositioning

• Automatic lowering prior to objective change

• Electronic parfocality

• Motorized and coded

• Left side ports (100%, 80%, or 50% transmission)

• Left side port dichroic splitting at 680 nm

• Right side ports (100%, 80%, or 50% transmission)

• Bottom port

Optional

• Top port with 2 switching positions

• 100% to eyepieces

• 50% to eyepieces/ 50% to port

14

4. Overview of the Instrument

Controls

Electronics Box
Leica CTR6000

Interfaces

•7 fixed control buttons for illumination and apertures

•7 variable function buttons behind the focus controls

•3 fixed control buttons for focus steps

•2 focus hand wheels

•7 buttons for fluorescence cubes and shutters

•4 buttons for magnification changers and ports

• SmartMove: ergonomic controller for x, y, z and 4 additional vari­able function buttons

• Separate control unit for all motorized and electronic elements of
the microscope such as:

• Objective turret

• Ffocus

• Ports

• Magnification changer

• Fluorescence

• Condenser

• Motorized stages
With

• Power supply for 100W halogen lamp

• Power supply for SmartMove

•2 x RS232C

•2 x USB

•4 x external/internal peripherals

Software Tools

• Leica Application Suite (LAS) for Windows
For:

• Microscope and camera configuration

• Microscope and camera control

• Image acquisition

TM

2000, XP with plug-ins

15

4. Overview of the Instrument

4.2 Glossary
The Stand

Four basic versions of the Leica DMI stand (DMI
3000 B, DMI 4000 B, DMI 5000 B, DMI 6000 B) are
available which can be combined into a wide
range of microscope variants.

The basic building blocks of the Leica
DMI6000 B stand are:

• DMI6000 B electronic stand

• Integrated fluorescence axis with motorized
filter cube changer (6 positions)

• With or without bottom port

• Lateral camera ports, 100%, 80%, or 50%

• Optional top port at the left side of the tube

• Integrated motorized tube lens changer

• Optional Bertrand lens

The individual variants and their components,
differences, and applications are described in
this manual. The function and performance of all
microscopy techniques and required accesso­ries of the Leica DMI6000 B will be described in
detail in the section of this manual that covers
the operation of the microscope.

Tube

The tube and tube lens create the primary image
together with the objective.
The tubes are an integral part of the stand and
consist of a basic body and a binocular section.
The trinocular tube also features a photo/video
port. A switchable mirror diverts either 100% of
the light to the eyepieces or camera port, or
splits it, with 50% each going to the eyepieces
and camera port.
A Bertrand lens is also available as an option.

Eyepieces

The eyepieces create an enlarged, virtual image
of the actual intermediate image created by the
objective. The eyepiece serves as a magnifier in
this respect.

Intensity Controller

The stand contains a 12V 100 W transformer for
continuous regulation of the intensity via the in­tensity controller.
The intensity can be adjusted using the controls
(1.6).

Focus Wheel

The focus wheel allows quick, precise focusing
of the microscopic image. Focusing is realized
by the vertical travel of the objective turret, with
a total range of 9mm.

Incident Light-Fluorescence Unit

The stand features an integrated fluorescence
axis and a motorized filter cube changer with
6 positions.

Aperture Ddiaphragm

The aperture diaphragm determines the resolu­tion, depth of field, and contrast of the micro­scopic image. The best resolution is obtained
when the apertures of the objective and the
condenser are roughly the same.

The aperture diaphragm in the illumination
light path is not for setting the image bright­ness. This should be done only with the inten­sity controls or neutral filters.

Caution:

16

4. Overview of the Instrument

Condenser

The condenser is a lens system that gathers
light and projects it onto the specimen from
above. The condenser is designed for the utiliza­tion of the numerical aperture in the objective.

Condenser Height Adjustment

The markings of the transmitted-light column indi­cate the height to be set for the used condenser.

Stages and Accessories

The stage is designed to accommodate the
specimens to be observed. Mechanical and mo­torized 3-plate cross-stages are available for the
Leica DMI 6000 B.

Motorized Objective Turret and Objectives

The motorized objective turret is designed to ac­commodate the objectives. The L-objectives
with their long working distances especially
take into consideration the correction of varying
container bottom thicknesses.
All microscope objectives are usable, from

1.25:1 to 100 : 1 magnification. All objectives in

the Leica product range with a 25mm thread and
coverslip correction are compatible. For perfor­mance data on Leica objectives, please refer to
the most current valid objective lists available
from your Leica representative.

Transmitted Light Illumination Unit

The transmitted light illumination unit consists of
an illumination carrier and the transmitted light
illumination column. The transmitted light illumi­nation carrier does not contain a lamp housing,
but a filter module for two swing-in filters and,
depending on the condenser used, a field dia­phragm.

Filter

The filters are generally used to enhance the
contrast of the specimen and are installed in the
illumination carrier. A selection of various filters
can be interchanged as required.

Field Diaphragm

The field diaphragm is used to realize Koehler il­lumination.

Lamp Housing for Transmitted Light

The lamp housings 107/2 and 107 (both for 12V
100W halogen) are available for the Leica DMI
6000 B. For their descriptions and applications,
please refer to the section of this manual de­scribing the use of the microscope.
The letter L indicates a lamp housing designed
for left-handed operation.

Lamp Housing for Incident Light

The 106 z L lamp housing (for halogen or xenon)
is available for the Leica DMI 6000 B. For their
descriptions and applications, please refer to
the section of this manual describing the use of
the microscope.
The letter L indicates a lamp housing designed
for left-handed operation.

Leica CTR6000 Electronics Box

The Leica CTR6000 electronics box contains the
power supply for the lamp and the circuit boards
required to control the motorized functions of
the stand.

17

4. Overview of the Instrument

18

17

1

14

Fig. 1 Leica DMI 6000 B left view
1 Eyepiece
2 Eyepiece tube
3 Top port
4 Intermediate pupil interface
5 LeicaScreen
6 Light intensity
7 Field diaphragm
8 TL/IL switching
9 Aperture diaphragm
10 Focus wheel

16

2

15

3

4

5

678910111213

11 Variable function buttons
12 Right side port
13 Booster lens
14 Lamp mount
15 Condenser head
16 Condenser base
17 Field diaphragm
18 Transmitted light lamp housing
19 DIC objective prism disk

18

4. Overview of the Instrument

11

4

5

Fig. 2 Leica DMI 6000 B right view
1 E-focus control buttons
2 Focus wheel
3 Variable function buttons
4 Opener for drawer
5 Drawer
6 Right side port

12 3

67812910

7 Analyzer slot
8 Centering window
9 Field diaphragm centering
10 Incident light lamp housing
11 Objective turret
12 Stage with attachable mechanical stage

19

4. Overview of the Instrument

4

3

5

6

2

Fig. 3 Leica DMI 6000 B front view
1 LeicaScreen
2 Front control panel
3 Port switching
4 Top port
5 Manual transmitted light filters
6 Field diaphragm centering

20

1

Fig. 3b SmartMove remote control module
1 Travel in x
2 Travel in y
3 Focus

Fig. 3a Front control panel

Fig. 4 Overall view of Leica DMI 6000 B with SmartMove remote control module

4 Variable function buttons

(preassigned at factory)

4. Overview of the Instrument

3

1

2

4

21

5. Unpacking the Microscope

5. Unpacking the Microscope

The microscope is delivered in several packages.

The stand package contains the following com­ponents:

• Stand with integrated incident light axis,
objective turret and tube

• Illumination arm

• Specimen stage

• CD with Leica Application Suite (LAS)
software package

• Instructions and list of microscope presets
(identification sheet)

The system package contains the microscope's
accessories:

• Eyepieces

• Objectives

• Condenser

• Lamp housings with accessories

• Assembly tools

• Additional accessories such as filter cubes,
etc. depending on feature set

The Leica CTR6000 electronics box, the Smart­Move remote control module and the ebq 100
supply unit are supplied in separate packages.

22

Please carefully compare the contents of the
delivery to the packing slip, delivery note, or in­voice. We urgently recommend storing a copy of
these documents with the manual to ensure that
you have information on the time and scope of
delivery handy for subsequent orders or service
work.
Please make sure that no small parts remain in the
packing material. Parts of our packing material are
marked by symbols to simplify recycling.

First, carefully remove all components from
the transportation and packaging materials.

Do not put the instrument into operation in
the event of visible damage to the compo­nents or packing material.

Caution!

5. Unpacking the Microscope

Caution!

Do not connect the microscope or periph­erals to an AC power source at this time
under any circumstances!

Installation Location

Work with the microscope should be performed
in a dust-free room, which is free of oil vapors
and other chemical vapors, as well as extreme
humidity. At the workplace, large temperature
fluctuations, direct sunlight, and vibrations
should be avoided. These may adversely affect
measurements and long-term observations.

Allowable ambient conditions
Temperature 15-35°C
Relative humidity maximum 80% up to 30°C

Note:

If at all possible, avoid touching the lens surfac­es of the objectives. If fingerprints do appear on
the glass surfaces, remove them with a soft
leather or linen cloth. Even small traces of finger
perspiration can damage the surfaces in a short
time. See the chapter "Care of the Microscope"

p. 95, for additional instructions.

→

Microscopes in warm and warm-damp climatic
zones require special care in order to prevent
the build up of fungus.
See the chapter "Care of the Microscope"
→ p. 95, for additional instructions.

Electrical components must be placed at
least 10 cm away from the wall and away
from flammable substances.

Caution!

23

5. Unpacking the Microscope

Transport

For shipping or transporting the microscope and
its accessory components, the original packag­ing should be used.

As a precaution to prevent damage from vibra­tions, the following components should be dis­assembled and packaged separately:

• Unscrew the objectives.

• Remove the eyepieces.

• Remove the condenser.

• Remove the specimen stage.

• Remove the transmitted light arm.

• Remove the lamp housings.

• Remove the lamp housing mount.

• Disassemble the burner of 106 z lamp housing.

• Remove the filter cube.

• Remove all moving or loose parts.

24

6. Assembling the Microscope

6. Assembly

The microscope components are logically as­sembled in this order:

•Transmitted light illumination carrier

• DIC module and DIC objective prisms*

• Specimen stage

• Condenser with condenser head

• Eyepieces

• Objectives

•Transmitted light lamps

• Lamp housing mount (mirror housings)

• Incident light lamps

• Assembly of incident light turret disk*

• Polarizer and analyzer*

When using intermediate systems and optical
accessories, the sequence may vary.
In this case, read Chapter
"6.15 Optional Accessories" → p. 52.

6.1 Assembly Tools

If possible, the microscope should be assem­bled and set up with the assistance of Leica
sales or service personnel.
A small number of universal screwdrivers which
are included in the scope of delivery are re­quired for assembly (Fig. 7).

Fig. 7 Assembly tools
1 Phillips screwdriver*

2

3mm Allen key

3 1.5mm centering key*
4 2mm centering key*
5 3mm hex key*
6 2.5mm hex key* (short type)
7 2.5mm hex key*

* depending on scope of delivery

2

5

1

3

4

6

2

7

25

6. Assembly

6.2 Installation of the
Transmitted Light Illumination Carrier (DL)

Wipe the installation surface (8.3) with a dry
cloth. Tip the illumination carrier (8.1) back
slightly and install it so that the pin (8.2) engages
the groove in the support surface (8.4).

Set the DL illumination carrier upright and fasten
it with the 4 screws.

When fastening the transmitted light illumina­tion carrier, do not hold it so as to ensure its op­timal alignment with the optical axis.
The tilt angle of the illumination carrier can be
varied with the knurled screw (9.1) or fixed verti­cally. Connect the electronics cable to one of
the sockets, EXT1 - EXT4.

The transmitted light lamp housing for 12V 100W
halogen lamps is a separate component. For in­structions on replacing the halogen lamp
→ Ch. 6.10, p. 41.

26

6. Assembly

6.3 Installation of the DIC Module

and DIC Objective Prisms

If your microscope is not equipped with DIC,
please continue with Chapter 6.4.

In the Leica DMI 6000 B microscope, the DIC
prisms are already installed in the DIC disk be­low the objective turret (Fig. 10b).

Proceed as follows when making changes to the IC
prism disk:

• Remove the front cover (Fig. 11) below the

objective revolver after releasing the socket
screws (Fig. 10a).

Fig. 10a Removing the front cover

Fig. 11 Front cover, DIC prism disk

• Insert the DIC prism disk (Fig. 10b) in its
receptacle and tighten the two socket
screws.
Note: insert the prism disk with the
electronics board facing down.

Replacing Individual IC Prisms:

• Release the two socket screws and remove
the prism disk.

• Place the prism against the stop pin (10b.3),
place the washer between the screw and the
prism, and tighten gently to prevent undue
tension. Insert the prism so that its identifying
letter, e.g. ID, is facing upward and is legible.

• After installing the prisms, replace the prism
disk into its receptacle.

Fig. 10b DIC objective prism turret (coded and motorized)
1 IC objective prism in frame
2 Identification letter (ID)
3 Orientation pin

Fig. 12 IC objective prism
1 Objective prism in frame
2 Screw and washer

1

2

321

27

6. Assembly

6.4 Installation of Specimen Stages

A wide range of specimen stages are available.
The most important are the following:

• Fixed stage (248mm x 204mm):
normal, heating and temperature-controlled

• Fixed micromanipulation stage (248mm x 204/
112mm): normal, heating, temperature­controlled

• Regular manual 3-plate cross-stage, position­ing range: 83mm x 127mm

• Manual micromanipulation 3-plate cross-stage
positioning range: 40mm x 40mm

• Motorized micromanipulation 3-plate cross­stage
positioning range: 40mm x 40mm

• Manual rotating stage

• Scanning stage IM 120 x 100
(motors on top)

• Scanning stage IM 120 x 100
(motors on bottom)

Fig. 14 Mechanical 3-plate stage

Fig. 15 Micromanipulation stage with attachable mechan-

ical stage

Fig. 13 Fixed stage (normal)

28

Fig. 16 3-plate micromanipulation stage

6. Assembly

The assembly of these stages is identical. The
stages are solidly attached to the microscope by
three screws. In the case of fixed stages, an at­tachable mechanical stage may be installed
(Fig. 18). These are supplied in a separate pack­age.

Multiple-plate stages are supplied separately.
Like the fixed stages, these stages are mounted
as follows:

• If the screws for the stage are already in the
stand, remove them first. In most cases, the
screws will be found in the packing material
of the stand.

Caution!

!

The screw lengths may vary. When using
screws of different lengths, use the shorter of
the three screws in the front hole and the
equally long ones in the rear holes.

• Use a clean cloth to remove dust and packing
material residue from the stand’s contact
surface with the stage.

• Align the stage so that the pair of holes faces
back toward the illumination axis and the
single hole faces forward toward the tube.

• Align the mounting holes in the stage with the
holes in the support surface. If the holes are
covered, in the case of 3-plate cross-stages
or scanning stages, please shift the upper
stage plate until the opening becomes visible.

• First, tighten the single front screw with the
included 3mm hex screwdriver. Be sure to use

shortest of the three screws in the front

the
hole, as an excessively long screw can
interfere with the focus travel. (If you have a
rotating stage, please continue reading under
"Rotating Stage and Insert Frame for Cover­slips").

Fig. 17 Fixed micromanipulation stage Fig. 18 Attachable mechanical stage for fixed microman-

ipulation stage

29

6. Assembly

• Next, firmly tighten the two rear screws.

• Finally, give the front screw a final firm
tightening.

Fixed Stage

Attachable mechanical stages that are designed
to accept a variety of culture dishes are also
available for fixed stages. These mechanical
stages may be attached to either side of the
fixed stage (Fig. 17).

Two screws are located at the underside (right
or left) of the attachable mechanical stage.
Tighten these screws in the threaded holes on
the underside of the fixed stage with the 3mm
hex screwdriver. Retighten these screws from
time to time after frequent use.

The attachable mechanical stage has been pre­adjusted in the factory. In the event that the at­tachable mechanical stage runs out of focus
when moving from right to left, this can be cor­rected by Leica’s technical service.

Next, remove one or more of the ordered insert
frames (Fig. 20) from their packaging and place
the insert frame into the precise retention sys­tem. The stage, the attachable mechanical
stage, and the insert frame are now ready for
use.

Some (not all) inserts are provided with self-ad­hesive scales to allow the coordinates to be
read.

Apply these scales to the recesses of the at­tachable mechanical stage.

Fig. 20 a, b, c

Inserts for attachable mechanical stage (micromanipulation
stage)

a

Fig. 19 a, b

Inserts for attachable mechanical stage (fixed stage)

30

a

b

b

c